MC11g1093.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC11g1093.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	DUF2431 domain-containing protein
Location	MC11: 9340113 .. 9341669 (-)
Sequence length	594
RNA-Seq Expression	MC11g1093.1
Synteny	MC11g1093.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGGTAATGCACTACAATAGCTCCCACAAAATACTTCTCGTTGGAGAGGGCAACTTCTCCTTCTCGGTTTGCTTAGCCACCGCCCTCGGCTCGGGTGACAACATCGTCGCCACGTCCCTCGACTCCAAAAGTATTTCAACGAAAGAATTTCACTACCTAACATAAATTTAGTATATCATAAACTTTACGATTACGCTAACATATTTACTTGACGCTATTTATGTTATAAGAGACACTTTTGAGGAAGTACGGGGACGAAATCGAGACGACTTTGGAAGAGTTGGAGGAGGAAGGTTGCATTGTGATGCATGGAGTGAATGTAAGAACGATGAGCCAACATCCTCTTTTGCGCAATGAGTTATTTGATCGCATTGTGTTCAATTTCCCACATGCTGGCTTCACATACTCAGAAATTGAACCCAAGCAAATCAAGTGAGTATACAAAAAAAATGTCTTTAAATTCTTAATTATCTGAATTTGTAATGATTTTAATTTCTTTTTTTTTTTTATCTTGTTGGATTTGGAATGAGATTTGAAATGTAGTTATTTTGTTTGTGTGTTTGGACATTTATGTTTATTGGAGAGGGAAAATAAAAATTTACTAAATATCTTCATTTATTAATTGAAAATTAAAGTATAATAGGATTGTAACTTAACCCTCGTACCCTATTAACATATTTTAGTTTGGAGATTTTTTTTTTTTCCTTTTTCAAGAGGTCATTTTAAGAGATATGTGAGAAGCATTACAAAAAAATAACACTCCAATTAAAGGAAACGGCAGATATGTTATAATTAAAAAAAAAAAAGTTTCTCTGGGCAAATTACACAAAAATATGTATTTTTAAAAACAATTTTAGTACTTTTATTTAATTTTCTTTAGAAGTGTCATAGACTTAACTTTTTTTTCTTTTTTTTTTTCCTTCTCACTCGATATTTCAGCAAAGCTTAACTTTTTAGGGAGATGGTCTGGAAGATAAACTAATTCAATACAAACAAAAAATATGAGATTGATGAAGTTTTTTTTTTTCTTGAGAAAATAAATAAACACTTAAAAATAACTCTAAATCAGATTTAACTTAAGAAATAAGCCAGATTGATGAAGTTTATAACATACCCAAAAACAATATTGGAAGACTTAAAAAAAAACACAAGGATATTCCTGCTATTGTCCCCCCACACCCACAACTAAGGACTTTTTCCTCTCGGGTAGGAGAATCATGATCGAAATAGCAAATCCAAAACTACGAAACTCGTGTTTTTAAGTCGTCTCGGTTTTGTGTGTTTGTGTTGGGCAGACTCCACCAGAATCTAGTGAGATGTTTTGTGAGGAATGCAGTGGAAATGGTGTCTGAAAATGGTGAAATTCACATCACCCACAAGACTTCACATCCATTCAGTGAATGGGAGATTGTGGAAATTGCAGAAGAAGAGGGTTTGTTTTTGAAAGAAGAAGCAGAGTTCAGCAAATGGGATTATCCAGGTTATATCAATAAGAAAGGCGATGGGCCTTATTGCAACCGCACTTTCCCTGTGGGATGTTGCTGCACTTTCAAGTTT

mRNA sequence

TGGGTAATGCACTACAATAGCTCCCACAAAATACTTCTCGTTGGAGAGGGCAACTTCTCCTTCTCGGTTTGCTTAGCCACCGCCCTCGGCTCGGGTGACAACATCGTCGCCACGTCCCTCGACTCCAAAAAGACACTTTTGAGGAAGTACGGGGACGAAATCGAGACGACTTTGGAAGAGTTGGAGGAGGAAGGTTGCATTGTGATGCATGGAGTGAATGTAAGAACGATGAGCCAACATCCTCTTTTGCGCAATGAGTTATTTGATCGCATTGTGTTCAATTTCCCACATGCTGGCTTCACATACTCAGAAATTGAACCCAAGCAAATCAAACTCCACCAGAATCTAGTGAGATGTTTTGTGAGGAATGCAGTGGAAATGGTGTCTGAAAATGGTGAAATTCACATCACCCACAAGACTTCACATCCATTCAGTGAATGGGAGATTGTGGAAATTGCAGAAGAAGAGGGTTTGTTTTTGAAAGAAGAAGCAGAGTTCAGCAAATGGGATTATCCAGGTTATATCAATAAGAAAGGCGATGGGCCTTATTGCAACCGCACTTTCCCTGTGGGATGTTGCTGCACTTTCAAGTTT

Coding sequence (CDS)

TGGGTAATGCACTACAATAGCTCCCACAAAATACTTCTCGTTGGAGAGGGCAACTTCTCCTTCTCGGTTTGCTTAGCCACCGCCCTCGGCTCGGGTGACAACATCGTCGCCACGTCCCTCGACTCCAAAAAGACACTTTTGAGGAAGTACGGGGACGAAATCGAGACGACTTTGGAAGAGTTGGAGGAGGAAGGTTGCATTGTGATGCATGGAGTGAATGTAAGAACGATGAGCCAACATCCTCTTTTGCGCAATGAGTTATTTGATCGCATTGTGTTCAATTTCCCACATGCTGGCTTCACATACTCAGAAATTGAACCCAAGCAAATCAAACTCCACCAGAATCTAGTGAGATGTTTTGTGAGGAATGCAGTGGAAATGGTGTCTGAAAATGGTGAAATTCACATCACCCACAAGACTTCACATCCATTCAGTGAATGGGAGATTGTGGAAATTGCAGAAGAAGAGGGTTTGTTTTTGAAAGAAGAAGCAGAGTTCAGCAAATGGGATTATCCAGGTTATATCAATAAGAAAGGCGATGGGCCTTATTGCAACCGCACTTTCCCTGTGGGATGTTGCTGCACTTTCAAGTTT

Protein sequence

WVMHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEELEEEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCFVRNAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGDGPYCNRTFPVGCCCTFKF

Homology

[Close]

BLAST of MC11g1093.1 vs. ExPASy Swiss-Prot
Match: P0C8L4 (Uncharacterized protein At4g26485 OS=Arabidopsis thaliana OX=3702 GN=At4g26485 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 3.5e-50
Identity = 104/201 (51.74%), Postives = 138/201 (68.66%), Query Frame = 0


Query: 1   WVMHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEE 60
           W+ HY+S+HKILLVGEGNFSFS+CLA+A GS  NI ATSLDS+  L  KY D ++  +  
Sbjct: 7   WIRHYSSTHKILLVGEGNFSFSLCLASAFGSAMNITATSLDSEDELSIKYMDAVD-NINI 66

Query: 61  LEEEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAG--FTYSEIEPKQIKLHQNLVR 120
           L+  GC + H V+V TMS    L  + +DRIVFNFPHAG  F   E+  + I+ H+ LVR
Sbjct: 67  LKRYGCDIQHEVDVHTMSFDNSLSLQRYDRIVFNFPHAGSRFFGRELSSRAIESHKELVR 126

Query: 121 CFVRNAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKK 180
            F+ NA EM+ E+GEIHITHKT++PFS+W I ++ + EGL L ++++F    YPGYI K+
Sbjct: 127 GFLENAKEMLEEDGEIHITHKTTYPFSDWGIKKLGKGEGLKLLKKSKFELSHYPGYITKR 186

Query: 181 GD-GPYCNRTFPVGCCCTFKF 199
           G  G   +  FPVG C T+ F
Sbjct: 187 GSGGRRSDDYFPVGECSTYMF 206

[Close]

BLAST of MC11g1093.1 vs. ExPASy Swiss-Prot
Match: F4I1X0 (Heavy metal-associated isoprenylated plant protein 41 OS=Arabidopsis thaliana OX=3702 GN=HIPP41 PE=3 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 1.9e-48
Identity = 95/198 (47.98%), Postives = 129/198 (65.15%), Query Frame = 0


Query: 1   WVMHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEE 60
           WV HY+S+H+ILLVGEG+FSFS  LAT  GS  NI A+SLDS   ++RKY  +  + L+ 
Sbjct: 20  WVKHYSSNHQILLVGEGDFSFSHSLATLFGSASNICASSLDSYDVVVRKY-KKARSNLKT 79

Query: 61  LEEEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCF 120
           L+  G +++HGV+  T+  HP LR   FDR++FNFPHAGF   E +   I+ H+ LV  F
Sbjct: 80  LKRLGALLLHGVDATTLHFHPDLRYRRFDRVIFNFPHAGFHGRESDSSLIRKHRELVFGF 139

Query: 121 VRNAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGD 180
              A  ++  NGE+H++HK   PFSEW + E+A    L L +   F K +YPGY NK+GD
Sbjct: 140 FNGASRLLRANGEVHVSHKNKAPFSEWNLEELASRCFLVLIQRVAFEKNNYPGYENKRGD 199

Query: 181 GPYCNRTFPVGCCCTFKF 199
           G  C++ F +G C TFKF
Sbjct: 200 GRRCDQPFLLGECSTFKF 216

[Close]

BLAST of MC11g1093.1 vs. ExPASy Swiss-Prot
Match: O94480 (25S rRNA (uridine-N(3))-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bmt5 PE=3 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.1e-18
Identity = 56/182 (30.77%), Postives = 100/182 (54.95%), Query Frame = 0


Query: 1   WVMHYNSSHKILLVGEGNFSFSVCLATALGSGDN-IVATSLDSKKTLLRKYGDEIETTLE 60
           +V+ +  +++ LL+GEGNFSF+  L     S +  ++ATS DSK+ L +KY D  E  + 
Sbjct: 50  YVLPFEKNNRFLLLGEGNFSFAFSLLLHHVSSEGFVLATSYDSKEDLKQKYPDAAE-YIS 109

Query: 61  ELEEEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRC 120
           ++E  G  VMH ++   +  H  L+ + FD I +NFPH+G    + + + I  +Q ++  
Sbjct: 110 KIEINGGKVMHEIDATKLHLHKKLKTQKFDTIFWNFPHSGKGIKD-QDRNILDNQKMLLA 169

Query: 121 FVRNAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKG 180
           F + +  ++SE G I IT   + P++ W +  +A++ G       +F    YP Y +++ 
Sbjct: 170 FFKASKFLLSEKGVIVITLAETKPYTLWNLKGLAKDAGYTSLMTEKFDSSFYPEYSHRRT 229

Query: 181 DG 182
            G
Sbjct: 230 IG 229

[Close]

BLAST of MC11g1093.1 vs. ExPASy Swiss-Prot
Match: Q3UY23 (Ferredoxin-fold anticodon-binding domain-containing protein 1 homolog OS=Mus musculus OX=10090 GN=Fdxacb1 PE=2 SV=2)

HSP 1 Score: 72.8 bits (177), Expect = 4.9e-12
Identity = 52/177 (29.38%), Postives = 82/177 (46.33%), Query Frame = 0


Query: 10  KILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIE-TTLEELEEEGCIV 69
           ++LLVGEGNFSF+  L   L    ++ AT    +  L    GD +    L+ L E G  V
Sbjct: 5   RLLLVGEGNFSFAASLIDGLDPSVSVTATGFQHRAAL---EGDPVALENLKRLRERGVEV 64

Query: 70  MHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCFVRNAVEMV 129
             GV+   +S      +  FDRI FNFPH G          +  ++ L+  F ++  +++
Sbjct: 65  RFGVDCTQLSHALPADDRDFDRIYFNFPHCG------RKAGVAKNRELLAKFFQSCADVL 124

Query: 130 SENGEIHIT----------HKTSHPF-SEWEIVEIAEEEGLFLKEEAEFSKWDYPGY 175
           ++ GE+H+T           K    + + W++V +A   G  L +   FS    PGY
Sbjct: 125 AKAGEVHVTLCRGQGGTPADKPQREWHNSWQVVAMAALGGFILSDVCPFSCEAVPGY 172

[Close]

BLAST of MC11g1093.1 vs. ExPASy Swiss-Prot
Match: Q9BRP7 (Ferredoxin-fold anticodon-binding domain-containing protein 1 OS=Homo sapiens OX=9606 GN=FDXACB1 PE=1 SV=3)

HSP 1 Score: 66.2 bits (160), Expect = 4.6e-10
Identity = 51/177 (28.81%), Postives = 80/177 (45.20%), Query Frame = 0


Query: 10  KILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIE-TTLEELEEEGCIV 69
           ++LLVGEGNFSF+  L+  L     + AT L     L R   D +    L+ L E G  V
Sbjct: 5   RLLLVGEGNFSFAAALSETLDQSTQLTATCLQRPAELAR---DPLAWENLQCLRERGIDV 64

Query: 70  MHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCFVRNAVEMV 129
             GV+   ++    L    FD+I F FPH G          +  ++ L+  F ++  +++
Sbjct: 65  RFGVDCTQLADVFELHEREFDQIYFIFPHCG------RKAGVAKNRELLAKFFQSCADVL 124

Query: 130 SENGEIHI----------THKTSHPF-SEWEIVEIAEEEGLFLKEEAEFSKWDYPGY 175
           +E GE+H+            K    + + W++V +A   GL L +   FS     GY
Sbjct: 125 AEEGEVHVALCRGQGGTPADKPQREWHNSWQVVAMAALGGLILSDVYPFSCKAVAGY 172

[Close]

BLAST of MC11g1093.1 vs. NCBI nr
Match: XP_022139694.1 (uncharacterized protein At4g26485-like [Momordica charantia])

HSP 1 Score: 414 bits (1064), Expect = 8.20e-146
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 0


Query: 3   MHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEELE 62
           MHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEELE
Sbjct: 1   MHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEELE 60

Query: 63  EEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCFVR 122
           EEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCFVR
Sbjct: 61  EEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCFVR 120

Query: 123 NAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGDGP 182
           NAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGDGP
Sbjct: 121 NAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGDGP 180

Query: 183 YCNRTFPVGCCCTFKF 198
           YCNRTFPVGCCCTFKF
Sbjct: 181 YCNRTFPVGCCCTFKF 196

[Close]

BLAST of MC11g1093.1 vs. NCBI nr
Match: XP_022976038.1 (uncharacterized protein At4g26485 isoform X1 [Cucurbita maxima])

HSP 1 Score: 305 bits (782), Expect = 1.08e-102
Identity = 146/199 (73.37%), Postives = 168/199 (84.42%), Query Frame = 0


Query: 1   WVMHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEE 60
           W+ HY+SSH ILLVGEGNFSFS CLA+A G+  N+VATSLDS++TLLRKYG +I+TTLEE
Sbjct: 8   WITHYSSSHDILLVGEGNFSFSACLASAFGTATNMVATSLDSEETLLRKYGSDIKTTLEE 67

Query: 61  LEEEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYS-EIEPKQIKLHQNLVRC 120
           L+E GC V+HGV+V TMSQH LL +  FDRIVFNFPHAGF YS E E  QIKLHQNLVR 
Sbjct: 68  LKELGCSVIHGVDVATMSQHLLLCHTWFDRIVFNFPHAGFQYSREHETGQIKLHQNLVRS 127

Query: 121 FVRNAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKG 180
           F+RNA E+++ENGEIHITHK S+P+SEW+I +IAEEEGLFL+EE EF KWDYP Y NKKG
Sbjct: 128 FLRNAKELLAENGEIHITHKISYPYSEWKIEKIAEEEGLFLREEVEFDKWDYPCYDNKKG 187

Query: 181 DGPYCNRTFPVGCCCTFKF 198
            GP  NRTFPVG CCTFKF
Sbjct: 188 SGPNSNRTFPVGLCCTFKF 206

[Close]

BLAST of MC11g1093.1 vs. NCBI nr
Match: KAG7024996.1 (hypothetical protein SDJN02_13816, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 305 bits (781), Expect = 1.65e-102
Identity = 144/198 (72.73%), Postives = 166/198 (83.84%), Query Frame = 0


Query: 1   WVMHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEE 60
           W+ HY SSH ILLVGEGNFSFS CLA+A G+  N+VATSLDS++TLLRKYG +I+TTLEE
Sbjct: 8   WITHYGSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSDIKTTLEE 67

Query: 61  LEEEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCF 120
           L+E GC VMHGV+V TMSQHPLL + LFDRIVFNFPHAGF YSE E +QIKLHQNLVR F
Sbjct: 68  LKELGCSVMHGVDVTTMSQHPLLCHTLFDRIVFNFPHAGFQYSEHETRQIKLHQNLVRRF 127

Query: 121 VRNAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGD 180
           +RNA E+++ENG+IHITHK S P+SEWEI E+AEEE LFL+E  EF+  DYPGY+NKKG 
Sbjct: 128 LRNAKELLAENGDIHITHKISFPYSEWEIEEVAEEEDLFLRELVEFNIGDYPGYVNKKGS 187

Query: 181 GPYCNRTFPVGCCCTFKF 198
           GP  N TFPVG C TFKF
Sbjct: 188 GPNSNLTFPVGLCSTFKF 205

[Close]

BLAST of MC11g1093.1 vs. NCBI nr
Match: XP_022936898.1 (uncharacterized protein At4g26485 isoform X1 [Cucurbita moschata])

HSP 1 Score: 299 bits (766), Expect = 3.16e-100
Identity = 143/198 (72.22%), Postives = 164/198 (82.83%), Query Frame = 0


Query: 1   WVMHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEE 60
           W+ HY+SSH ILLVGEGNFSFS CLA+A G+  N+VATSLDS++TLLRKYG +I+TTLEE
Sbjct: 8   WITHYSSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSDIKTTLEE 67

Query: 61  LEEEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCF 120
           L+E GC VMHGV+V TMSQHPLL + LFDRIVFNFPHAGF YS    K  KLHQNLVR F
Sbjct: 68  LKELGCSVMHGVDVMTMSQHPLLCHTLFDRIVFNFPHAGFQYSR--DKSSKLHQNLVRRF 127

Query: 121 VRNAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGD 180
           +RNA E+  ENGEIHITHK S+P+SEWEI E+AEEE LFL+EE EF++ DYPGY+NKKG 
Sbjct: 128 LRNAKELFVENGEIHITHKISYPYSEWEIEEVAEEEDLFLREEVEFNEGDYPGYVNKKGS 187

Query: 181 GPYCNRTFPVGCCCTFKF 198
           GP  NRTF VG C TFKF
Sbjct: 188 GPNSNRTFAVGLCSTFKF 203

[Close]

BLAST of MC11g1093.1 vs. NCBI nr
Match: XP_024200090.2 (uncharacterized protein At4g26485 [Rosa chinensis])

HSP 1 Score: 273 bits (697), Expect = 8.96e-90
Identity = 121/197 (61.42%), Postives = 160/197 (81.22%), Query Frame = 0


Query: 2   VMHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEEL 61
           +MHY+S+ KILLVGEGNFSF+ CLA   GS  N+VA+SLDSK++++ +Y +   + L+EL
Sbjct: 14  IMHYSSNQKILLVGEGNFSFAACLAKEFGSAKNMVASSLDSKESVMIRYSNAAPSNLKEL 73

Query: 62  EEEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCFV 121
           E+ GC+++H V+V TM QHPLL N+LFDR+VFNFPHAGF + E +  QIKLHQ+L+R F 
Sbjct: 74  EDMGCVILHEVDVHTMRQHPLLVNQLFDRVVFNFPHAGFIFMENQMSQIKLHQDLMRGFF 133

Query: 122 RNAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGDG 181
            +A EM+ E+GE+H+THKTSHPFS+WEIV++AEE GL+L EEA F++ DYPGY+NK+G G
Sbjct: 134 TSACEMLKESGEVHVTHKTSHPFSKWEIVKLAEETGLYLVEEASFTRGDYPGYLNKRGSG 193

Query: 182 PYCNRTFPVGCCCTFKF 198
             CNRTFPVG C T+KF
Sbjct: 194 KKCNRTFPVGQCSTYKF 210

[Close]

BLAST of MC11g1093.1 vs. ExPASy TrEMBL
Match: A0A6J1CDR1 (uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC111010540 PE=4 SV=1)

HSP 1 Score: 414 bits (1064), Expect = 3.97e-146
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 0


Query: 3   MHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEELE 62
           MHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEELE
Sbjct: 1   MHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEELE 60

Query: 63  EEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCFVR 122
           EEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCFVR
Sbjct: 61  EEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCFVR 120

Query: 123 NAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGDGP 182
           NAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGDGP
Sbjct: 121 NAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGDGP 180

Query: 183 YCNRTFPVGCCCTFKF 198
           YCNRTFPVGCCCTFKF
Sbjct: 181 YCNRTFPVGCCCTFKF 196

[Close]

BLAST of MC11g1093.1 vs. ExPASy TrEMBL
Match: A0A6J1IKX9 (uncharacterized protein At4g26485 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476560 PE=4 SV=1)

HSP 1 Score: 305 bits (782), Expect = 5.24e-103
Identity = 146/199 (73.37%), Postives = 168/199 (84.42%), Query Frame = 0


Query: 1   WVMHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEE 60
           W+ HY+SSH ILLVGEGNFSFS CLA+A G+  N+VATSLDS++TLLRKYG +I+TTLEE
Sbjct: 8   WITHYSSSHDILLVGEGNFSFSACLASAFGTATNMVATSLDSEETLLRKYGSDIKTTLEE 67

Query: 61  LEEEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYS-EIEPKQIKLHQNLVRC 120
           L+E GC V+HGV+V TMSQH LL +  FDRIVFNFPHAGF YS E E  QIKLHQNLVR 
Sbjct: 68  LKELGCSVIHGVDVATMSQHLLLCHTWFDRIVFNFPHAGFQYSREHETGQIKLHQNLVRS 127

Query: 121 FVRNAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKG 180
           F+RNA E+++ENGEIHITHK S+P+SEW+I +IAEEEGLFL+EE EF KWDYP Y NKKG
Sbjct: 128 FLRNAKELLAENGEIHITHKISYPYSEWKIEKIAEEEGLFLREEVEFDKWDYPCYDNKKG 187

Query: 181 DGPYCNRTFPVGCCCTFKF 198
            GP  NRTFPVG CCTFKF
Sbjct: 188 SGPNSNRTFPVGLCCTFKF 206

[Close]

BLAST of MC11g1093.1 vs. ExPASy TrEMBL
Match: A0A6J1FEI5 (uncharacterized protein At4g26485 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443349 PE=4 SV=1)

HSP 1 Score: 299 bits (766), Expect = 1.53e-100
Identity = 143/198 (72.22%), Postives = 164/198 (82.83%), Query Frame = 0


Query: 1   WVMHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEE 60
           W+ HY+SSH ILLVGEGNFSFS CLA+A G+  N+VATSLDS++TLLRKYG +I+TTLEE
Sbjct: 8   WITHYSSSHDILLVGEGNFSFSACLASAFGTATNVVATSLDSEETLLRKYGSDIKTTLEE 67

Query: 61  LEEEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCF 120
           L+E GC VMHGV+V TMSQHPLL + LFDRIVFNFPHAGF YS    K  KLHQNLVR F
Sbjct: 68  LKELGCSVMHGVDVMTMSQHPLLCHTLFDRIVFNFPHAGFQYSR--DKSSKLHQNLVRRF 127

Query: 121 VRNAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGD 180
           +RNA E+  ENGEIHITHK S+P+SEWEI E+AEEE LFL+EE EF++ DYPGY+NKKG 
Sbjct: 128 LRNAKELFVENGEIHITHKISYPYSEWEIEEVAEEEDLFLREEVEFNEGDYPGYVNKKGS 187

Query: 181 GPYCNRTFPVGCCCTFKF 198
           GP  NRTF VG C TFKF
Sbjct: 188 GPNSNRTFAVGLCSTFKF 203

[Close]

BLAST of MC11g1093.1 vs. ExPASy TrEMBL
Match: A0A6J1CDH1 (uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC111010537 PE=4 SV=1)

HSP 1 Score: 270 bits (690), Expect = 7.52e-89
Identity = 128/198 (64.65%), Postives = 154/198 (77.78%), Query Frame = 0


Query: 1   WVMHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEE 60
           W+ HY+SSH ILLVGEG+FSFS CLAT  GS  N+VATSLDSK++LL KY   + T L+E
Sbjct: 12  WIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSKY-THVATNLKE 71

Query: 61  LEEEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCF 120
           LEE GC V H V+  +MSQHPLLR++ FDR+VFNFPHAGF + E + +QI+LH++LVR F
Sbjct: 72  LEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQIELHRHLVRGF 131

Query: 121 VRNAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGD 180
           +RNA E + E GEIHITHKT+HPFS WEIVE+A EEGL LKEE  F  W+YP Y NK+GD
Sbjct: 132 LRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEYPNYENKRGD 191

Query: 181 GPYCNRTFPVGCCCTFKF 198
           G   + TFPVG C TFKF
Sbjct: 192 GWNSDGTFPVGACRTFKF 208

[Close]

BLAST of MC11g1093.1 vs. ExPASy TrEMBL
Match: A0A2P6Q8B4 (Putative S-adenosyl-L-methionine-dependent methyltransferase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr5g0024501 PE=4 SV=1)

HSP 1 Score: 266 bits (679), Expect = 2.70e-87
Identity = 121/201 (60.20%), Postives = 159/201 (79.10%), Query Frame = 0


Query: 2   VMHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKK----TLLRKYGDEIETT 61
           +MHY+S+ KILLVGEGNFSF+ CLA   GS  N+VA+SLDSK     +++ +Y +   + 
Sbjct: 14  IMHYSSNQKILLVGEGNFSFAACLAKEFGSAKNMVASSLDSKGNKNVSVMIRYSNAAPSN 73

Query: 62  LEELEEEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLV 121
           L+ELE+ GC+++H V+V TM QHPLL N+LFDR+VFNFPHAGF + E +  QIKLHQ+L+
Sbjct: 74  LKELEDMGCVILHEVDVHTMRQHPLLVNQLFDRVVFNFPHAGFIFMENQMSQIKLHQDLM 133

Query: 122 RCFVRNAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINK 181
           R F  +A EM+ E+GE+H+THKTSHPFS+WEIV++AEE GL+L EEA F++ DYPGY+NK
Sbjct: 134 RGFFTSACEMLKESGEVHVTHKTSHPFSKWEIVKLAEETGLYLVEEASFTRGDYPGYLNK 193

Query: 182 KGDGPYCNRTFPVGCCCTFKF 198
           +G G  CNRTFPVG C T+KF
Sbjct: 194 RGSGKKCNRTFPVGQCSTYKF 214

[Close]

BLAST of MC11g1093.1 vs. TAIR 10
Match: AT1G55790.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 193.7 bits (491), Expect = 1.4e-49
Identity = 95/198 (47.98%), Postives = 129/198 (65.15%), Query Frame = 0


Query: 1   WVMHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEE 60
           WV HY+S+H+ILLVGEG+FSFS  LAT  GS  NI A+SLDS   ++RKY  +  + L+ 
Sbjct: 20  WVKHYSSNHQILLVGEGDFSFSHSLATLFGSASNICASSLDSYDVVVRKY-KKARSNLKT 79

Query: 61  LEEEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCF 120
           L+  G +++HGV+  T+  HP LR   FDR++FNFPHAGF   E +   I+ H+ LV  F
Sbjct: 80  LKRLGALLLHGVDATTLHFHPDLRYRRFDRVIFNFPHAGFHGRESDSSLIRKHRELVFGF 139

Query: 121 VRNAVEMVSENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGD 180
              A  ++  NGE+H++HK   PFSEW + E+A    L L +   F K +YPGY NK+GD
Sbjct: 140 FNGASRLLRANGEVHVSHKNKAPFSEWNLEELASRCFLVLIQRVAFEKNNYPGYENKRGD 199

Query: 181 GPYCNRTFPVGCCCTFKF 199
           G  C++ F +G C TFKF
Sbjct: 200 GRRCDQPFLLGECSTFKF 216

[Close]

BLAST of MC11g1093.1 vs. TAIR 10
Match: AT5G56060.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 177.2 bits (448), Expect = 1.3e-44
Identity = 98/189 (51.85%), Postives = 126/189 (66.67%), Query Frame = 0


Query: 2   VMHYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEEL 61
           +  Y++  KILLVGEG+FSFS+ LA   GS  NI ATSLD+++ L  KY D  +  +E L
Sbjct: 9   LQQYSNKQKILLVGEGDFSFSLSLARVFGSATNITATSLDTREELGIKYTDG-KANVEGL 68

Query: 62  EEEGCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTY-SEIEPKQIKLHQNLVRCF 121
           E  GC V+HGVNV +MS     R   +DRI+FNFPH+G  + SE +   I LHQ LVR F
Sbjct: 69  ELFGCTVVHGVNVHSMSSD--YRLGRYDRIIFNFPHSGLGFGSEHDIFFIMLHQGLVRGF 128

Query: 122 VRNAVEMV-SENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKG 181
           + +A +M+  E+GEIH+THKT+ PF+ W I  +A E+GL L  E EF KW +PGY NKKG
Sbjct: 129 LESARKMLKDEDGEIHVTHKTTDPFNRWGIETLAGEKGLRLIGEIEFHKWAFPGYSNKKG 188

Query: 182 DGPYCNRTF 189
            G  CN TF
Sbjct: 189 GGSNCNSTF 194

[Close]

BLAST of MC11g1093.1 vs. TAIR 10
Match: AT5G56075.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 160.2 bits (404), Expect = 1.7e-39
Identity = 88/199 (44.22%), Postives = 122/199 (61.31%), Query Frame = 0


Query: 4   HYNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEELEE 63
           HY +  KILLVGEG+FSFS+ LA A GS  N+ ATSLD++  L +K+ +  +  +EELE 
Sbjct: 69  HYTNKQKILLVGEGDFSFSLSLARAFGSASNLTATSLDTQGELEQKFKNG-KANVEELER 128

Query: 64  EGCIVMHGVNVRTMSQHPLL-RNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCFVR 123
            GC V++GVNV +M   P +  + ++DR++FNFP                   LVR F++
Sbjct: 129 LGCSVVYGVNVHSMITKPSVGGSAIYDRVIFNFP----------------THELVRGFMK 188

Query: 124 NAVEMVSE---NGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKG 183
           +A  +V +    GEIH+ HKT +PFSEW++  + E+EGL L  E EF    YPGY NK+G
Sbjct: 189 SARVLVKDEDKGGEIHVIHKTEYPFSEWKLKTLGEKEGLDLIREVEFCLSHYPGYFNKRG 248

Query: 184 DGPYCNRTFPVGCCCTFKF 199
            G Y + +FPVG   TF F
Sbjct: 249 SGGYSDSSFPVGKSSTFMF 250

[Close]

BLAST of MC11g1093.1 vs. TAIR 10
Match: AT4G26485.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 147.5 bits (371), Expect = 1.1e-35
Identity = 80/168 (47.62%), Postives = 109/168 (64.88%), Query Frame = 0


Query: 34  NIVATSLDSKKTLLRKYGDEIETTLEELEEEGCIVMHGVNVRTMSQHPLLRNELFDRIVF 93
           NI ATSLDS+  L  KY D ++  +  L+  GC + H V+V TMS    L  + +DRIVF
Sbjct: 2   NITATSLDSEDELSIKYMDAVD-NINILKRYGCDIQHEVDVHTMSFDNSLSLQRYDRIVF 61

Query: 94  NFPHAG--FTYSEIEPKQIKLHQNLVRCFVRNAVEMVSENGEIHITHKTSHPFSEWEIVE 153
           NFPHAG  F   E+  + I+ H+ LVR F+ NA EM+ E+GEIHITHKT++PFS+W I +
Sbjct: 62  NFPHAGSRFFGRELSSRAIESHKELVRGFLENAKEMLEEDGEIHITHKTTYPFSDWGIKK 121

Query: 154 IAEEEGLFLKEEAEFSKWDYPGYINKKGD-GPYCNRTFPVGCCCTFKF 199
           + + EGL L ++++F    YPGYI K+G  G   +  FPVG C T+ F
Sbjct: 122 LGKGEGLKLLKKSKFELSHYPGYITKRGSGGRRSDDYFPVGECSTYMF 168

[Close]

BLAST of MC11g1093.1 vs. TAIR 10
Match: AT5G25030.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 146.0 bits (367), Expect = 3.3e-35
Identity = 84/195 (43.08%), Postives = 118/195 (60.51%), Query Frame = 0


Query: 5   YNSSHKILLVGEGNFSFSVCLATALGSGDNIVATSLDSKKTLLRKYGDEIETTLEELEEE 64
           Y++  KIL+VGEG FSFS+ LA ALGS  NI A SLD ++ L R Y +  +  +EELE  
Sbjct: 12  YSNEQKILVVGEGEFSFSLSLAKALGSATNITAISLDIREDLGRNYNNG-KGNVEELERL 71

Query: 65  GCIVMHGVNVRTMSQHPLLRNELFDRIVFNFPHAGFTYSEIEPKQIKLHQNLVRCFVRNA 124
           GC V+ GVNV +M     L +  +D I+FNFPHAG        K+ K+       F+ +A
Sbjct: 72  GCTVVRGVNVHSMKSDDRLAH--YDIIIFNFPHAG--------KRNKVFGG----FMESA 131

Query: 125 VEMV-SENGEIHITHKTSHPFSEWEIVEIAEEEGLFLKEEAEFSKWDYPGYINKKGDGPY 184
            EM+  E+GEIHIT  T +PF++W++  +AEE GL L +  +F KW +P   NK+  G  
Sbjct: 132 REMMKDEDGEIHITLNTLNPFNKWDLKALAEESGLRLIQRMQFIKWAFPSSSNKRESGSN 191

Query: 185 CNRTFPVGCCCTFKF 199
           C+  +P+G   T+ F
Sbjct: 192 CDFIYPIGSAITYMF 191

The following BLAST results are available for this feature:

BLAST of MC11g1093.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0C8L4	3.5e-50	51.74	Uncharacterized protein At4g26485 OS=Arabidopsis thaliana OX=3702 GN=At4g26485 P...	[more]
F4I1X0	1.9e-48	47.98	Heavy metal-associated isoprenylated plant protein 41 OS=Arabidopsis thaliana OX...	[more]
O94480	2.1e-18	30.77	25S rRNA (uridine-N(3))-methyltransferase OS=Schizosaccharomyces pombe (strain 9...	[more]
Q3UY23	4.9e-12	29.38	Ferredoxin-fold anticodon-binding domain-containing protein 1 homolog OS=Mus mus...	[more]
Q9BRP7	4.6e-10	28.81	Ferredoxin-fold anticodon-binding domain-containing protein 1 OS=Homo sapiens OX...	[more]

BLAST of MC11g1093.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139694.1	8.20e-146	100.00	uncharacterized protein At4g26485-like [Momordica charantia]	[more]
XP_022976038.1	1.08e-102	73.37	uncharacterized protein At4g26485 isoform X1 [Cucurbita maxima]	[more]
KAG7024996.1	1.65e-102	72.73	hypothetical protein SDJN02_13816, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022936898.1	3.16e-100	72.22	uncharacterized protein At4g26485 isoform X1 [Cucurbita moschata]	[more]
XP_024200090.2	8.96e-90	61.42	uncharacterized protein At4g26485 [Rosa chinensis]	[more]

BLAST of MC11g1093.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CDR1	3.97e-146	100.00	uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1IKX9	5.24e-103	73.37	uncharacterized protein At4g26485 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1FEI5	1.53e-100	72.22	uncharacterized protein At4g26485 isoform X1 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1CDH1	7.52e-89	64.65	uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A2P6Q8B4	2.70e-87	60.20	Putative S-adenosyl-L-methionine-dependent methyltransferase OS=Rosa chinensis O...	[more]

BLAST of MC11g1093.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55790.1	1.4e-49	47.98	Domain of unknown function (DUF2431)	[more]
AT5G56060.1	1.3e-44	51.85	Domain of unknown function (DUF2431)	[more]
AT5G56075.1	1.7e-39	44.22	Domain of unknown function (DUF2431)	[more]
AT4G26485.1	1.1e-35	47.62	Domain of unknown function (DUF2431)	[more]
AT5G25030.1	3.3e-35	43.08	Domain of unknown function (DUF2431)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019446	Domain of unknown function DUF2431	PFAM	PF10354	DUF2431	coord: 12..177 e-value: 2.7E-47 score: 161.2
None	No IPR available	PANTHER	PTHR11538:SF80	SUBFAMILY NOT NAMED	coord: 2..198
None	No IPR available	PANTHER	PTHR11538	PHENYLALANYL-TRNA SYNTHETASE	coord: 2..198
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 73..157

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC11g1093	MC11g1093	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g1093.1-cds	MC11g1093.1-cds-MC11:9340113..9340374	CDS
MC11g1093.1-cds	MC11g1093.1-cds-MC11:9341238..9341439	CDS
MC11g1093.1-cds	MC11g1093.1-cds-MC11:9341540..9341669	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC11g1093.1	MC11g1093.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0008168	methyltransferase activity