MC11g1071.1 (mRNA) Bitter gourd (Dali-11) v1

BLAST of MC11g1071.1 vs. ExPASy Swiss-Prot
Match: Q8VYM6 (26S rRNA (cytosine-C(5))-methyltransferase NOP2B OS=Arabidopsis thaliana OX=3702 GN=NOP2B PE=1 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 1.6e-189
Identity = 379/641 (59.13%), Postives = 468/641 (73.01%), Query Frame = 0

Query: 9   KKGVKRNDPPSKTK----------KKEELFTAKRSKEEEENDSDL-----SDGLGDAEVE 68
           KK  K   PP K +          KK E+ T +  +EEE   SD      SD   D + E
Sbjct: 29  KKAAKTQKPPLKKQRKCISEKKPLKKPEVSTDEEEEEEENEQSDEGSESGSDLFSDGDEE 88

Query: 69  -NGVLDDDDGFGSGSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKS 128
            N   DDDD      +   +  +PLA+DFL GSD E  +    GS   SDS    +  KS
Sbjct: 89  GNNDSDDDDDDDDDDDDDDEDAEPLAEDFLDGSDNEEVT---MGSDLDSDSGGSKLERKS 148

Query: 129 RAIDEERARVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREIVRV 188
           RAID +R +  ++A+DE K+NI+E+ DEF+LP ++ELEEE  RPPDL +LQ RIREIVR+
Sbjct: 149 RAIDRKRKKEVQDADDEFKMNIKEKPDEFQLPTQKELEEEARRPPDLPSLQMRIREIVRI 208

Query: 189 LTNFKALRQEG--ATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKP 248
           L+NFK L+ +G    R DYV QLK D+ SYYGYNEFL G L+E+FPV+ELMELIEAFEK 
Sbjct: 209 LSNFKDLKPKGDKHERNDYVGQLKADLSSYYGYNEFLIGTLIEMFPVVELMELIEAFEKK 268

Query: 249 RPICLRTNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYM 308
           RP  +RTNTLKTRRRDLAD+L+NRGVNLDPLSKWSKVGL+VYDSQVPIGATPEY+AGFYM
Sbjct: 269 RPTSIRTNTLKTRRRDLADILLNRGVNLDPLSKWSKVGLIVYDSQVPIGATPEYLAGFYM 328

Query: 309 LQGASSFLPVMALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTA 368
           LQ ASSFLPVMALAP+EKER++DMAAAPGGKTTY+AALMKN+GII+ANEMK PRLKSL+A
Sbjct: 329 LQSASSFLPVMALAPREKERVVDMAAAPGGKTTYVAALMKNTGIIYANEMKVPRLKSLSA 388

Query: 369 NLHRMGVTNTVVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQ 428
           NLHRMGVTNT+VC+YDG+EL KV+G ++ DRVLLDAPCSG+GV+SKDESVKTSKS ++I+
Sbjct: 389 NLHRMGVTNTIVCNYDGRELTKVLGQSSVDRVLLDAPCSGTGVISKDESVKTSKSADDIK 448

Query: 429 KCSKLQKELILAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGLD 488
           K + LQK+LIL AID+ DANSK+GGY+VYSTCS+++ ENEAVIDYALK RDVKLVPCGLD
Sbjct: 449 KFAHLQKQLILGAIDLVDANSKTGGYIVYSTCSVMIPENEAVIDYALKNRDVKLVPCGLD 508

Query: 489 FGRPGFIRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSDPLET--- 548
           FGRPGF  FRE RFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSN +  + + +P  T   
Sbjct: 509 FGRPGFSSFREHRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNAMQPSGNDEPAVTMEQ 568

Query: 549 --AMEEDNESDKSE--------------------NEENDGECLHPRKEQVEGKKRKEVLQ 607
                 D++ +K+E                     +E+  +  +PR +++   KR    +
Sbjct: 569 AQVSSSDDDDEKAEAIEELEKPPVASGQPKRESNTKEDTNKRKNPRSKEIHKGKRN---K 628

BLAST of MC11g1071.1 vs. ExPASy Swiss-Prot
Match: Q9FG73 (25S rRNA (cytosine-C(5))-methyltransferase NOP2A OS=Arabidopsis thaliana OX=3702 GN=NOP2A PE=1 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 1.6e-186
Identity = 382/642 (59.50%), Postives = 470/642 (73.21%), Query Frame = 0

Query: 9   KKGVK-RNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVL---------D 68
           KKG K +N PP K +KKE +        E  +DSD  D + D   E+ +          +
Sbjct: 39  KKGAKSQNKPPLKKQKKEVVEEEPLEDYEVTDDSDEDDEVSDGSDEDDISPAVESEEIDE 98

Query: 69  DDDGFGSGSEVSSD-----GDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKSR 128
            DDG    +++ SD      ++ L DDFL+GS +E+  GS     S +DSD+DDIV KS 
Sbjct: 99  SDDGENGSNQLFSDDEEENDEETLGDDFLEGSGDEDEEGSLDAD-SDADSDDDDIVAKSD 158

Query: 129 AIDEERARVEKEAEDEMKLNIRE--------ESDEFRLPIKEELEEEVLRPPDLSNLQRR 188
           AID + A  +K+A  E++  I++        E D FRLP +EELEEE   PPDL  L+ R
Sbjct: 159 AIDRDLAMQKKDAAAELEDFIKQDDVHDEEPEHDAFRLPTEEELEEEARGPPDLPLLKTR 218

Query: 189 IREIVRVLTNFKALRQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIE 248
           I EIVR L NFKA R +  TRK  VEQLK D+GSYYGYN FL G L+E+FP  ELMELIE
Sbjct: 219 IEEIVRALKNFKAFRPKDTTRKACVEQLKADLGSYYGYNSFLIGTLVEMFPPGELMELIE 278

Query: 249 AFEKPRPICLRTNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYM 308
           AFEK RP  +RTNTLKTRRRDLADVL+NRGVNLDPLSKWSKVGLV+YDSQVPIGATPEY+
Sbjct: 279 AFEKQRPTSIRTNTLKTRRRDLADVLLNRGVNLDPLSKWSKVGLVIYDSQVPIGATPEYL 338

Query: 309 AGFYMLQGASSFLPVMALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRL 368
           AG+YMLQGASSFLPVMALAP+E ERI+D+AAAPGGKTTYIAALMKN+G+I+ANEMK PRL
Sbjct: 339 AGYYMLQGASSFLPVMALAPRENERIVDVAAAPGGKTTYIAALMKNTGLIYANEMKVPRL 398

Query: 369 KSLTANLHRMGVTNTVVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKS 428
           KSLTANLHRMGVTNT+VC+YDG+ELPKV+G NT DRVLLDAPCSG+G++SKDESVK +K+
Sbjct: 399 KSLTANLHRMGVTNTIVCNYDGRELPKVLGQNTVDRVLLDAPCSGTGIISKDESVKITKT 458

Query: 429 VEEIQKCSKLQKELILAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLV 488
           ++EI+K + LQK+L+LAAIDM DANSK+GGY+VYSTCSI+V ENEAVIDYALKKRDVKLV
Sbjct: 459 MDEIKKFAHLQKQLLLAAIDMVDANSKTGGYIVYSTCSIMVTENEAVIDYALKKRDVKLV 518

Query: 489 PCGLDFGRPGFIRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSD-- 548
            CGLDFGR GF RFRE RF PSL+KTRRFYPHVHNMDGFFVAKLKKMSN   ++E  D  
Sbjct: 519 TCGLDFGRKGFTRFREHRFQPSLDKTRRFYPHVHNMDGFFVAKLKKMSNVKQSSEEGDDD 578

Query: 549 ------PLETAMEEDNESDKSENEENDGECLHPRKEQVEGKKRKEVLQNHDVENG----- 608
                   E + ++D+E++  E  E     +   KE+ E K ++++ ++ + + G     
Sbjct: 579 AVETVEQAEVSSDDDDEAEAIEETEKPSVPVRQPKERKEKKNKEKLAKSKEDKRGKKDKK 638

BLAST of MC11g1071.1 vs. ExPASy Swiss-Prot
Match: P46087 (Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase OS=Homo sapiens OX=9606 GN=NOP2 PE=1 SV=2)

HSP 1 Score: 489.6 bits (1259), Expect = 5.3e-137
Identity = 304/623 (48.80%), Postives = 398/623 (63.88%), Query Frame = 0

Query: 6   FNKKKGVKRNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVLDDDDGFGS 65
           FN  +G KR  P S  +++EE       ++   N  DL     DA+       DD G  S
Sbjct: 98  FNAPRGKKRPAPGSDEEEEEE----DSEEDGMVNHGDLWGSEDDADTV-----DDYGADS 157

Query: 66  GSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKSRAIDEERARVEKE 125
            SE   +G+  L    ++ +  +  +   +  +  S+ + +D   +     E      +E
Sbjct: 158 NSEDEEEGEALLP---IERAARKQKAREAAAGIQWSEEETEDEEEEKEVTPESGPPKVEE 217

Query: 126 AEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREIVRVLTNFKALRQEGAT 185
           A+  +++N+ EE   F LP   E+E++  + PDL  + +RI++IV +L +F A R+EG +
Sbjct: 218 ADGGLQINVDEE--PFVLPPAGEMEQDA-QAPDLQRVHKRIQDIVGILRDFGAQREEGRS 277

Query: 186 RKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKPRPICLRTNTLKTRRR 245
           R +Y+ +LKKD+  YY Y +FL G LM+LFP+ EL+E +EA E PRP+ LRTNTLKTRRR
Sbjct: 278 RSEYLNRLKKDLAIYYSYGDFLLGKLMDLFPLSELVEFLEANEVPRPVTLRTNTLKTRRR 337

Query: 246 DLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPVMALAP 305
           DLA  LINRGVNLDPL KWSK GLVVYDS VPIGATPEY+AG YMLQGASS LPVMALAP
Sbjct: 338 DLAQALINRGVNLDPLGKWSKTGLVVYDSSVPIGATPEYLAGHYMLQGASSMLPVMALAP 397

Query: 306 QEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRMGVTNTVVCSY 365
           QE ERI+DM  APGGKT+Y+A LMKN+G+I AN+  A RLKS+  NLHR+GVTNT++  Y
Sbjct: 398 QEHERILDMCCAPGGKTSYMAQLMKNTGVILANDANAERLKSVVGNLHRLGVTNTIISHY 457

Query: 366 DGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQKCSKLQKELILAAID 425
           DG++ PKV+G    DRVLLDAPCSG+GV+SKD +VKT+K  ++I +C+ LQKEL+L+AID
Sbjct: 458 DGRQFPKVVG--GFDRVLLDAPCSGTGVISKDPAVKTNKDEKDILRCAHLQKELLLSAID 517

Query: 426 MADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGLDFGRPGFIRFREKRFH 485
             +A SK+GGY+VY TCSI V+ENE V+DYALKKR+V+LVP GLDFG+ GF RFRE+RFH
Sbjct: 518 SVNATSKTGGYLVYCTCSITVEENEWVVDYALKKRNVRLVPTGLDFGQEGFTRFRERRFH 577

Query: 486 PSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIP---TAESSDPLETAMEEDNESDKSENEE 545
           PSL  TRRFYPH HNMDGFF+AK KK SN IP   T  S     T ++      KSEN  
Sbjct: 578 PSLRSTRRFYPHTHNMDGFFIAKFKKFSNSIPQSQTGNSETATPTNVDLPQVIPKSENSS 637

Query: 546 NDGECLHPRKEQVEGKKRKEVLQN------------HDVENGEESTPAT-EKRKKRKFPS 605
                  P K+     K K+ LQ             + +  G +S  +T     K +  S
Sbjct: 638 ------QPAKKAKGAAKTKQQLQKQQHPKKASFQKLNGISKGADSELSTVPSVTKTQASS 697

Query: 606 RMEISKAREEKRQALREKKAGGK 613
             + S     K + +RE K  GK
Sbjct: 698 SFQDSSQPAGKAEGIREPKVTGK 697

BLAST of MC11g1071.1 vs. ExPASy Swiss-Prot
Match: Q922K7 (Probable 28S rRNA (cytosine-C(5))-methyltransferase OS=Mus musculus OX=10090 GN=Nop2 PE=1 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 9.0e-137
Identity = 287/572 (50.17%), Postives = 382/572 (66.78%), Query Frame = 0

Query: 5   KFNKKKGVKRNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVLDDDDGFG 64
           K NK  G+K   P   +K   +    KR    + +D D  +  G    E+ V+   D +G
Sbjct: 64  KPNKSPGIK-TLPGELSKGAVQARGKKRPAPIQNSDGDEEEDSG----EDDVVTQGDLWG 123

Query: 65  SGSEVSSDGDDPLADDFLQGSDEEN---------------GSGSGSGSVSGSDSDEDDIV 124
                S D D+ + DD+   S+ E+                +   +  V  ++ D D+  
Sbjct: 124 -----SEDSDEDMVDDYGAASNSEDEEEKLLPIERAALKQKAQDATAGVLWNEEDTDEDE 183

Query: 125 NKSRAIDEERARVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREI 184
           +      E   R + +AE ++++N+ E+ + F LP   E +++  + PDL  + +RI++I
Sbjct: 184 DDDGVSPESHPRKDDKAEGDLQINV-EDEEAFVLPPAGETDQDG-QAPDLQRVHKRIQDI 243

Query: 185 VRVLTNFKALRQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEK 244
           V VL +F A R+EG +R +Y+ +L+KD+ +YY Y +FL   LMELFP+ EL+E +EA E 
Sbjct: 244 VGVLRDFGAQREEGRSRAEYLSRLQKDLATYYSYGDFLLSKLMELFPLSELIEFLEANEV 303

Query: 245 PRPICLRTNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFY 304
           PRPI LRTNTLKTRRRDLA +LINRGVNLDPL KWSK GLVVYDS VPIGATPEY+AG Y
Sbjct: 304 PRPITLRTNTLKTRRRDLAQLLINRGVNLDPLGKWSKSGLVVYDSSVPIGATPEYLAGHY 363

Query: 305 MLQGASSFLPVMALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLT 364
           MLQGASS LPVMALAPQE ERI+DM  APGGKT+YIA LMKN+G+I AN+  A RLKS+ 
Sbjct: 364 MLQGASSMLPVMALAPQEHERILDMCCAPGGKTSYIAQLMKNTGVILANDANADRLKSVV 423

Query: 365 ANLHRMGVTNTVVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEI 424
            NLHR+GVTNT++  YDG++ PKV+G    DRVLLDAPCSG+GV+SKD +VKT+K  ++I
Sbjct: 424 GNLHRLGVTNTIISHYDGRQFPKVVG--GFDRVLLDAPCSGTGVISKDPAVKTNKDEKDI 483

Query: 425 QKCSKLQKELILAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGL 484
           Q+C+ LQKEL+L+AID  +A SK+GGY+VY TCSI V+ENE V+DYALKKR+V+LVP GL
Sbjct: 484 QRCAHLQKELLLSAIDSVNAASKTGGYLVYCTCSITVEENEWVVDYALKKRNVRLVPTGL 543

Query: 485 DFGRPGFIRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAES-SDPLETA 544
           DFG+ GF RF+ +RFHP+L  TRRFYPH HNMDGFF+AK KK SN IP   + +    T 
Sbjct: 544 DFGQEGFTRFQARRFHPTLRSTRRFYPHTHNMDGFFIAKFKKFSNSIPQPHAGNSAAATP 603

Query: 545 MEEDNESDKSENEENDGECLHPRKEQVEGKKR 561
            E D +   +   EN  +     +  V+ K++
Sbjct: 604 TEPDLKDQVTPKSENGSQPTKKARGAVKAKQQ 621

BLAST of MC11g1071.1 vs. ExPASy Swiss-Prot
Match: O94268 (25S rRNA (cytosine-C(5))-methyltransferase nop2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=nop2 PE=1 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 7.1e-134
Identity = 295/573 (51.48%), Postives = 386/573 (67.36%), Query Frame = 0

Query: 7   NKKKGVKRNDPPSKTKKKEELFTAKRS---KEEEENDSDLSDG--------LGDAEVENG 66
           N KK     + P  ++K+ +L   K+S     + EN+ DL D         L D E +  
Sbjct: 28  NAKKRKHSKEKPQNSRKR-QLAEEKKSLFENSDSENEKDLIDADEFEEAETLSDLEHDEE 87

Query: 67  VLDDDDGFGSGSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDED--DIVNKSR 126
                D F        +G++   +D +  SDEE+         SG + D +  ++   SR
Sbjct: 88  PQTFADEFIDDEAKECEGEE---EDSVFDSDEEHEVKPMFSDDSGDEEDLELANMEAMSR 147

Query: 127 AIDEERARVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLR--PPDLSNLQRRIREIVR 186
            +DEE    EKEAE+E+  NI  E+     PI    + + +   P DLS +Q RI+EIVR
Sbjct: 148 KLDEEAELEEKEAEEELHTNIHPEAPTVLPPIDGFTDSQPISTLPQDLSQIQLRIQEIVR 207

Query: 187 VLTNFKALRQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKPR 246
           VL +FK L + G  R +YV+QL  DI +YYGY+ FL   L ELF V E +E  EA E PR
Sbjct: 208 VLNDFKNLCEPGRNRSEYVDQLLNDICAYYGYSRFLAEKLFELFSVSEAVEFFEANEMPR 267

Query: 247 PICLRTNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYML 306
           P+ +RTNTLKT+RR+LA  LINRGVNL+P+ KWSKVGL V++SQVPIGATPEY+AG Y+L
Sbjct: 268 PVTIRTNTLKTQRRELAQALINRGVNLEPIGKWSKVGLQVFESQVPIGATPEYLAGHYIL 327

Query: 307 QGASSFLPVMALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTAN 366
           Q ASSFLPVMALAPQ  ERI+DM++APGGK TY+AAL KN+GIIFAN+    R K+L+AN
Sbjct: 328 QAASSFLPVMALAPQPNERILDMSSAPGGKVTYVAALQKNTGIIFANDSNKARTKALSAN 387

Query: 367 LHRMGVTNTVVCSYDGKELP-KVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQ 426
           +HR+GV N +VC+YDG++ P +V+G    DRVLLDAPCSG+GV+ KD+SVKT+KS  +  
Sbjct: 388 IHRLGVRNAIVCNYDGRKFPNEVIG--GFDRVLLDAPCSGTGVIYKDQSVKTNKSERDFD 447

Query: 427 KCSKLQKELILAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKR-DVKLVPCGL 486
             S LQ++L+L+AID  +A+SK+GG++VYSTCSI VDE+EAVI YALKKR +VKLV  GL
Sbjct: 448 TLSHLQRQLLLSAIDSVNADSKTGGFIVYSTCSITVDEDEAVIQYALKKRPNVKLVSTGL 507

Query: 487 DFGRPGFIRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSDPLETAM 546
           +FGR GF RFREKRFHPSL+ TRR+YPHVHN+DGFFVAKLKK+S+KIPT   +D ++   
Sbjct: 508 EFGREGFTRFREKRFHPSLKLTRRYYPHVHNIDGFFVAKLKKISDKIPTVNVADDMKDGT 567

Query: 547 EEDNESDKSENE-ENDGECLHPRKEQVEGKKRK 562
             D E +K+  E +N        KE +E  +RK
Sbjct: 568 NNDVEIEKNSTEIDNITFNDEADKEIIEQNRRK 594

BLAST of MC11g1071.1 vs. NCBI nr
Match: XP_022139523.1 (probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase [Momordica charantia])

HSP 1 Score: 1154 bits (2985), Expect = 0.0
Identity = 614/614 (100.00%), Postives = 614/614 (100.00%), Query Frame = 0

Query: 1   MAAAKFNKKKGVKRNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVLDDD 60
           MAAAKFNKKKGVKRNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVLDDD
Sbjct: 1   MAAAKFNKKKGVKRNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVLDDD 60

Query: 61  DGFGSGSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKSRAIDEERA 120
           DGFGSGSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKSRAIDEERA
Sbjct: 61  DGFGSGSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKSRAIDEERA 120

Query: 121 RVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREIVRVLTNFKALR 180
           RVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREIVRVLTNFKALR
Sbjct: 121 RVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREIVRVLTNFKALR 180

Query: 181 QEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKPRPICLRTNTL 240
           QEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKPRPICLRTNTL
Sbjct: 181 QEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKPRPICLRTNTL 240

Query: 241 KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV 300
           KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV
Sbjct: 241 KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV 300

Query: 301 MALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRMGVTNT 360
           MALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRMGVTNT
Sbjct: 301 MALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRMGVTNT 360

Query: 361 VVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQKCSKLQKELI 420
           VVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQKCSKLQKELI
Sbjct: 361 VVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQKCSKLQKELI 420

Query: 421 LAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGLDFGRPGFIRFR 480
           LAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGLDFGRPGFIRFR
Sbjct: 421 LAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGLDFGRPGFIRFR 480

Query: 481 EKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSDPLETAMEEDNESDKSEN 540
           EKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSDPLETAMEEDNESDKSEN
Sbjct: 481 EKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSDPLETAMEEDNESDKSEN 540

Query: 541 EENDGECLHPRKEQVEGKKRKEVLQNHDVENGEESTPATEKRKKRKFPSRMEISKAREEK 600
           EENDGECLHPRKEQVEGKKRKEVLQNHDVENGEESTPATEKRKKRKFPSRMEISKAREEK
Sbjct: 541 EENDGECLHPRKEQVEGKKRKEVLQNHDVENGEESTPATEKRKKRKFPSRMEISKAREEK 600

Query: 601 RQALREKKAGGKKG 614
           RQALREKKAGGKKG
Sbjct: 601 RQALREKKAGGKKG 614

BLAST of MC11g1071.1 vs. NCBI nr
Match: XP_038897895.1 (probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase [Benincasa hispida])

HSP 1 Score: 981 bits (2536), Expect = 0.0
Identity = 529/618 (85.60%), Postives = 569/618 (92.07%), Query Frame = 0

Query: 1   MAAAKFNKKKGVKRNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVLD-- 60
           MAAAKFNKKKGVK++DPPSK K+K+ELFT KRSKEE++ DSDLSD L + EV+NG++D  
Sbjct: 1   MAAAKFNKKKGVKQDDPPSKRKEKDELFTPKRSKEEDD-DSDLSDALDEDEVDNGIIDGD 60

Query: 61  --DDDGFGSGSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKSRAID 120
             D++G GSGSE SSDGDDPLA DFLQGSDEEN SGS    VS SDSDE DI +KSR ID
Sbjct: 61  EEDEEGSGSGSEDSSDGDDPLAHDFLQGSDEENDSGS----VSSSDSDETDIEDKSRIID 120

Query: 121 EERARVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREIVRVLTNF 180
           E+RAR EK+A DEM+LNI+EESDEFRLP KEELEEE LRPPDLSNLQRRIREIVRVL+NF
Sbjct: 121 EKRAREEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDLSNLQRRIREIVRVLSNF 180

Query: 181 KALRQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKPRPICLR 240
           KALRQEGATRKDYVEQLKKDIGSYYGYNEFL GVL+E+FPV+ELMELIEAFEKPRPICLR
Sbjct: 181 KALRQEGATRKDYVEQLKKDIGSYYGYNEFLIGVLVEMFPVVELMELIEAFEKPRPICLR 240

Query: 241 TNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASS 300
           TNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASS
Sbjct: 241 TNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASS 300

Query: 301 FLPVMALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRMG 360
           FLPVMALAPQEKERI+DMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRMG
Sbjct: 301 FLPVMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRMG 360

Query: 361 VTNTVVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQKCSKLQ 420
           VTNTVVC+YDGKELPKV+G NTADRVLLDAPCSGSGVVSKDESVKTSKS+EEIQKC++LQ
Sbjct: 361 VTNTVVCNYDGKELPKVLGVNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAELQ 420

Query: 421 KELILAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGLDFGRPGF 480
           K+LILAAIDM D NSKSGGYVVYSTCSI+VDENEAV+DYALKKRDVKLVPCGLDFGRPGF
Sbjct: 421 KQLILAAIDMVDCNSKSGGYVVYSTCSIMVDENEAVVDYALKKRDVKLVPCGLDFGRPGF 480

Query: 481 IRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSDPLETAMEEDNESD 540
           IRFREKRFHPSLEKTRRFYPHVHNMDGFFVAK KKMSNKI TA SS+ LE A+EEDN++ 
Sbjct: 481 IRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKFKKMSNKISTARSSESLEEAVEEDNDAG 540

Query: 541 KSENEENDGECLHPRKEQV-EGKKRKEVLQNHDVENGEESTPATEKRKKRKFPSRMEISK 600
           KS NE+NDGECLHP   QV + KKRK+V QNHDV NG+ES P  EKRKKRKFPSR EI++
Sbjct: 541 KSGNEKNDGECLHP---QVNDSKKRKKVSQNHDVGNGKESKPTVEKRKKRKFPSREEIAR 600

Query: 601 AREEKRQALREKKAGGKK 613
           AREEKRQALREKKAG KK
Sbjct: 601 AREEKRQALREKKAGNKK 610

BLAST of MC11g1071.1 vs. NCBI nr
Match: XP_022981419.1 (probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase [Cucurbita maxima])

HSP 1 Score: 981 bits (2536), Expect = 0.0
Identity = 532/622 (85.53%), Postives = 568/622 (91.32%), Query Frame = 0

Query: 1   MAAAKFNKKKGVKRNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVLDDD 60
           M AAKFNKKK VKR+DP SK KKK+ELFT KR K+EEENDSDLSD LG+ EVE+G+LD D
Sbjct: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALGEEEVEDGMLDGD 60

Query: 61  D-----GFGSGSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKSRAI 120
           +     G GSGSEVSSDGDDPLADDF  GSDEEN S S    VSGSDSDE DI  KSRAI
Sbjct: 61  ELGSGSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRS----VSGSDSDESDIEEKSRAI 120

Query: 121 DEERARVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREIVRVLTN 180
           DEERAR EK+A DEM+LNI+EESDEFRLP KEELEEE LRPPD+SNLQRRIREIVRVLTN
Sbjct: 121 DEERAREEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLTN 180

Query: 181 FKALRQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKPRPICL 240
           FKALRQEGATRKDYVEQLKKDIGSYYGYNEFLTGVL+ELFPV+ELMELIEAFEKPRPICL
Sbjct: 181 FKALRQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICL 240

Query: 241 RTNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGAS 300
           RTNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGAS
Sbjct: 241 RTNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGAS 300

Query: 301 SFLPVMALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRM 360
           SFLPVMALAPQEKERI+DMAAAPGGKTTYIAALMKNSGIIFANEMK PRLKSLTANLHRM
Sbjct: 301 SFLPVMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRM 360

Query: 361 GVTNTVVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQKCSKL 420
           GVTNTVVC+YDGKELPKV+G NTADRVLLDAPCSGSGV+SKDESVKTSKS+EEIQKC++L
Sbjct: 361 GVTNTVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVISKDESVKTSKSLEEIQKCAQL 420

Query: 421 QKELILAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGLDFGRPG 480
           QK+LILAAIDM DANSKSGGY+VYSTCSI+VDENEAV+DYAL+KR+VKLVPCGLDFGRPG
Sbjct: 421 QKQLILAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALRKRNVKLVPCGLDFGRPG 480

Query: 481 FIRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSDPLE--TAMEEDN 540
           FIRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPT +SS+ LE     EED 
Sbjct: 481 FIRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTTQSSEALEEEAVEEEDI 540

Query: 541 ESDKSENEENDGECLHPRKEQV-EGKKRKEVL-QNHDVENGEESTPATEKRKKRKFPSRM 600
           E++KS NE+ DGECLHP   QV +GKKRK+V+ + H+VENGEES P  EKRKKRKFPSR 
Sbjct: 541 EAEKSANEKTDGECLHP---QVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSRE 600

Query: 601 EISKAREEKRQALREKKAGGKK 613
           EISKAREEKRQALREKKAGG K
Sbjct: 601 EISKAREEKRQALREKKAGGSK 615

BLAST of MC11g1071.1 vs. NCBI nr
Match: XP_022941253.1 (probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase [Cucurbita moschata])

HSP 1 Score: 979 bits (2530), Expect = 0.0
Identity = 529/616 (85.88%), Postives = 566/616 (91.88%), Query Frame = 0

Query: 1   MAAAKFNKKKGVKRNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVLDDD 60
           M AAKFNKKK VKR+DP SK KKK+ELFT KR K+EEENDSDLSD L + EVE+G+LD D
Sbjct: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDEEEVEDGMLDGD 60

Query: 61  DGFGSGSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKSRAIDEERA 120
           +  GSGSEVSSDGDDPLADDF  GSDEEN S S    VSGSDSDE DI  KSRAIDEERA
Sbjct: 61  E-QGSGSEVSSDGDDPLADDFFHGSDEENVSRS----VSGSDSDESDIEEKSRAIDEERA 120

Query: 121 RVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREIVRVLTNFKALR 180
           R EK+A DEM+LNI+EESDEFRLP KEELEEE LRPPD+SNLQRRIREIVRVL+NFKALR
Sbjct: 121 REEKDAADEMQLNIKEESDEFRLPSKEELEEEALRPPDISNLQRRIREIVRVLSNFKALR 180

Query: 181 QEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKPRPICLRTNTL 240
           QEGATRKDYVEQLKKDIGSYYGYNEFLTGVL+ELFPV+ELMELIEAFEKPRPICLRTNTL
Sbjct: 181 QEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNTL 240

Query: 241 KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV 300
           KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV
Sbjct: 241 KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV 300

Query: 301 MALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRMGVTNT 360
           MALAP+EKERI+DMAAAPGGKTTYIAALMKNSGIIFANEMK PRLKSLTANLHRMGVTNT
Sbjct: 301 MALAPREKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNT 360

Query: 361 VVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQKCSKLQKELI 420
           VVC+YDGKELPKV+G NTADRVLLDAPCSGSGV+SKDESVKTSKS+EEIQKC++LQK+LI
Sbjct: 361 VVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVISKDESVKTSKSLEEIQKCAQLQKQLI 420

Query: 421 LAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGLDFGRPGFIRFR 480
           LAAIDM DANSKSGGY+VYSTCSI+VDENEAV+DYALKKR+VKLVPCGLDFGRPGFIRFR
Sbjct: 421 LAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFR 480

Query: 481 EKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSDPLE--TAMEEDNESDKS 540
           EKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTA+SS+ LE     EED E++KS
Sbjct: 481 EKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEEDIETEKS 540

Query: 541 ENEENDGECLHPRKEQVEGKKRKEVL-QNHDVENGEESTPATEKRKKRKFPSRMEISKAR 600
            NE NDGECLHP+    +GKKRK+V+ + H+VENGEES P  EKRKKRKFPSR EISKAR
Sbjct: 541 SNETNDGECLHPQAN--DGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAR 600

Query: 601 EEKRQALREKKAGGKK 613
           EEKRQALREKKAGG K
Sbjct: 601 EEKRQALREKKAGGSK 609

BLAST of MC11g1071.1 vs. NCBI nr
Match: KAG6607741.1 (26S rRNA (cytosine-C(5))-methyltransferase NOP2B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 979 bits (2530), Expect = 0.0
Identity = 529/619 (85.46%), Postives = 566/619 (91.44%), Query Frame = 0

Query: 1   MAAAKFNKKKGVKRNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVLDDD 60
           M AAKFNKKK VKR+DP SK KKK+ELFT KR K+EEENDSDLSD L + EVE+G+LD D
Sbjct: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDEEEVEDGMLDGD 60

Query: 61  D---GFGSGSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKSRAIDE 120
           +   G GSGSEVSSDGDDPLADDF  GSDEEN S S    VSGSDSDE DI  KSRAIDE
Sbjct: 61  EQGSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRS----VSGSDSDESDIEEKSRAIDE 120

Query: 121 ERARVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREIVRVLTNFK 180
           ERAR EK+A DEM+LNI+EESDEFRLP KEELEEE LRPPD+SNLQRRIREIVRVL+NFK
Sbjct: 121 ERAREEKDAADEMQLNIKEESDEFRLPSKEELEEEALRPPDISNLQRRIREIVRVLSNFK 180

Query: 181 ALRQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKPRPICLRT 240
           ALRQEGATRKDYVEQLKKDIGSYYGYNEFLTGVL+ELFP++ELMELIEAFEKPRPICLRT
Sbjct: 181 ALRQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPIVELMELIEAFEKPRPICLRT 240

Query: 241 NTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSF 300
           NTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSF
Sbjct: 241 NTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSF 300

Query: 301 LPVMALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRMGV 360
           LPVMALAPQEKERI+DMAAAPGGKTTYIAALMKNSGIIFANEMK PRLKSLTANLHRMGV
Sbjct: 301 LPVMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGV 360

Query: 361 TNTVVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQKCSKLQK 420
           TNTVVC+YDGKELPKV+G NTADRVLLDAPCSGSGV+SKDESVKTSKS+EEIQKC++LQK
Sbjct: 361 TNTVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVISKDESVKTSKSLEEIQKCAQLQK 420

Query: 421 ELILAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGLDFGRPGFI 480
           +LILAAIDM DANSKSGGY+VYSTCSI+VDENEAV+DYALKKR+VKLVPCGLDFGRPGFI
Sbjct: 421 QLILAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFI 480

Query: 481 RFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSDPLE--TAMEEDNES 540
           RFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTA SS+ LE     EED E+
Sbjct: 481 RFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTARSSEALEEEAVEEEDIEA 540

Query: 541 DKSENEENDGECLHPRKEQVEGKKRKEVL-QNHDVENGEESTPATEKRKKRKFPSRMEIS 600
           +KS NE N+GECLHP+    +GKKRK+V+ + H+VENGEES P  EKRKKRKFPSR EIS
Sbjct: 541 EKSSNETNNGECLHPQAN--DGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEIS 600

Query: 601 KAREEKRQALREKKAGGKK 613
           KAREEKRQALREKKAGG K
Sbjct: 601 KAREEKRQALREKKAGGSK 613

BLAST of MC11g1071.1 vs. ExPASy TrEMBL
Match: A0A6J1CFT7 (probable 28S rRNA (Cytosine(4447)-C(5))-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111010417 PE=3 SV=1)

HSP 1 Score: 1154 bits (2985), Expect = 0.0
Identity = 614/614 (100.00%), Postives = 614/614 (100.00%), Query Frame = 0

Query: 1   MAAAKFNKKKGVKRNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVLDDD 60
           MAAAKFNKKKGVKRNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVLDDD
Sbjct: 1   MAAAKFNKKKGVKRNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVLDDD 60

Query: 61  DGFGSGSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKSRAIDEERA 120
           DGFGSGSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKSRAIDEERA
Sbjct: 61  DGFGSGSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKSRAIDEERA 120

Query: 121 RVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREIVRVLTNFKALR 180
           RVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREIVRVLTNFKALR
Sbjct: 121 RVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREIVRVLTNFKALR 180

Query: 181 QEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKPRPICLRTNTL 240
           QEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKPRPICLRTNTL
Sbjct: 181 QEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKPRPICLRTNTL 240

Query: 241 KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV 300
           KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV
Sbjct: 241 KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV 300

Query: 301 MALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRMGVTNT 360
           MALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRMGVTNT
Sbjct: 301 MALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRMGVTNT 360

Query: 361 VVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQKCSKLQKELI 420
           VVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQKCSKLQKELI
Sbjct: 361 VVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQKCSKLQKELI 420

Query: 421 LAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGLDFGRPGFIRFR 480
           LAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGLDFGRPGFIRFR
Sbjct: 421 LAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGLDFGRPGFIRFR 480

Query: 481 EKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSDPLETAMEEDNESDKSEN 540
           EKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSDPLETAMEEDNESDKSEN
Sbjct: 481 EKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSDPLETAMEEDNESDKSEN 540

Query: 541 EENDGECLHPRKEQVEGKKRKEVLQNHDVENGEESTPATEKRKKRKFPSRMEISKAREEK 600
           EENDGECLHPRKEQVEGKKRKEVLQNHDVENGEESTPATEKRKKRKFPSRMEISKAREEK
Sbjct: 541 EENDGECLHPRKEQVEGKKRKEVLQNHDVENGEESTPATEKRKKRKFPSRMEISKAREEK 600

Query: 601 RQALREKKAGGKKG 614
           RQALREKKAGGKKG
Sbjct: 601 RQALREKKAGGKKG 614

BLAST of MC11g1071.1 vs. ExPASy TrEMBL
Match: A0A6J1J1T4 (probable 28S rRNA (Cytosine(4447)-C(5))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480547 PE=3 SV=1)

HSP 1 Score: 981 bits (2536), Expect = 0.0
Identity = 532/622 (85.53%), Postives = 568/622 (91.32%), Query Frame = 0

Query: 1   MAAAKFNKKKGVKRNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVLDDD 60
           M AAKFNKKK VKR+DP SK KKK+ELFT KR K+EEENDSDLSD LG+ EVE+G+LD D
Sbjct: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALGEEEVEDGMLDGD 60

Query: 61  D-----GFGSGSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKSRAI 120
           +     G GSGSEVSSDGDDPLADDF  GSDEEN S S    VSGSDSDE DI  KSRAI
Sbjct: 61  ELGSGSGSGSGSEVSSDGDDPLADDFFHGSDEENVSRS----VSGSDSDESDIEEKSRAI 120

Query: 121 DEERARVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREIVRVLTN 180
           DEERAR EK+A DEM+LNI+EESDEFRLP KEELEEE LRPPD+SNLQRRIREIVRVLTN
Sbjct: 121 DEERAREEKDAADEMQLNIKEESDEFRLPTKEELEEEALRPPDISNLQRRIREIVRVLTN 180

Query: 181 FKALRQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKPRPICL 240
           FKALRQEGATRKDYVEQLKKDIGSYYGYNEFLTGVL+ELFPV+ELMELIEAFEKPRPICL
Sbjct: 181 FKALRQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICL 240

Query: 241 RTNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGAS 300
           RTNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGAS
Sbjct: 241 RTNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGAS 300

Query: 301 SFLPVMALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRM 360
           SFLPVMALAPQEKERI+DMAAAPGGKTTYIAALMKNSGIIFANEMK PRLKSLTANLHRM
Sbjct: 301 SFLPVMALAPQEKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRM 360

Query: 361 GVTNTVVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQKCSKL 420
           GVTNTVVC+YDGKELPKV+G NTADRVLLDAPCSGSGV+SKDESVKTSKS+EEIQKC++L
Sbjct: 361 GVTNTVVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVISKDESVKTSKSLEEIQKCAQL 420

Query: 421 QKELILAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGLDFGRPG 480
           QK+LILAAIDM DANSKSGGY+VYSTCSI+VDENEAV+DYAL+KR+VKLVPCGLDFGRPG
Sbjct: 421 QKQLILAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALRKRNVKLVPCGLDFGRPG 480

Query: 481 FIRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSDPLE--TAMEEDN 540
           FIRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPT +SS+ LE     EED 
Sbjct: 481 FIRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTTQSSEALEEEAVEEEDI 540

Query: 541 ESDKSENEENDGECLHPRKEQV-EGKKRKEVL-QNHDVENGEESTPATEKRKKRKFPSRM 600
           E++KS NE+ DGECLHP   QV +GKKRK+V+ + H+VENGEES P  EKRKKRKFPSR 
Sbjct: 541 EAEKSANEKTDGECLHP---QVNDGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSRE 600

Query: 601 EISKAREEKRQALREKKAGGKK 613
           EISKAREEKRQALREKKAGG K
Sbjct: 601 EISKAREEKRQALREKKAGGSK 615

BLAST of MC11g1071.1 vs. ExPASy TrEMBL
Match: A0A6J1FRL9 (probable 28S rRNA (Cytosine(4447)-C(5))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446614 PE=3 SV=1)

HSP 1 Score: 979 bits (2530), Expect = 0.0
Identity = 529/616 (85.88%), Postives = 566/616 (91.88%), Query Frame = 0

Query: 1   MAAAKFNKKKGVKRNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVLDDD 60
           M AAKFNKKK VKR+DP SK KKK+ELFT KR K+EEENDSDLSD L + EVE+G+LD D
Sbjct: 1   MPAAKFNKKKAVKRDDPQSKRKKKDELFTPKRGKKEEENDSDLSDALDEEEVEDGMLDGD 60

Query: 61  DGFGSGSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKSRAIDEERA 120
           +  GSGSEVSSDGDDPLADDF  GSDEEN S S    VSGSDSDE DI  KSRAIDEERA
Sbjct: 61  E-QGSGSEVSSDGDDPLADDFFHGSDEENVSRS----VSGSDSDESDIEEKSRAIDEERA 120

Query: 121 RVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREIVRVLTNFKALR 180
           R EK+A DEM+LNI+EESDEFRLP KEELEEE LRPPD+SNLQRRIREIVRVL+NFKALR
Sbjct: 121 REEKDAADEMQLNIKEESDEFRLPSKEELEEEALRPPDISNLQRRIREIVRVLSNFKALR 180

Query: 181 QEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKPRPICLRTNTL 240
           QEGATRKDYVEQLKKDIGSYYGYNEFLTGVL+ELFPV+ELMELIEAFEKPRPICLRTNTL
Sbjct: 181 QEGATRKDYVEQLKKDIGSYYGYNEFLTGVLVELFPVVELMELIEAFEKPRPICLRTNTL 240

Query: 241 KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV 300
           KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV
Sbjct: 241 KTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLPV 300

Query: 301 MALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRMGVTNT 360
           MALAP+EKERI+DMAAAPGGKTTYIAALMKNSGIIFANEMK PRLKSLTANLHRMGVTNT
Sbjct: 301 MALAPREKERIVDMAAAPGGKTTYIAALMKNSGIIFANEMKVPRLKSLTANLHRMGVTNT 360

Query: 361 VVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQKCSKLQKELI 420
           VVC+YDGKELPKV+G NTADRVLLDAPCSGSGV+SKDESVKTSKS+EEIQKC++LQK+LI
Sbjct: 361 VVCNYDGKELPKVLGMNTADRVLLDAPCSGSGVISKDESVKTSKSLEEIQKCAQLQKQLI 420

Query: 421 LAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGLDFGRPGFIRFR 480
           LAAIDM DANSKSGGY+VYSTCSI+VDENEAV+DYALKKR+VKLVPCGLDFGRPGFIRFR
Sbjct: 421 LAAIDMVDANSKSGGYIVYSTCSIMVDENEAVVDYALKKRNVKLVPCGLDFGRPGFIRFR 480

Query: 481 EKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSDPLE--TAMEEDNESDKS 540
           EKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTA+SS+ LE     EED E++KS
Sbjct: 481 EKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAQSSEALEEEAVEEEDIETEKS 540

Query: 541 ENEENDGECLHPRKEQVEGKKRKEVL-QNHDVENGEESTPATEKRKKRKFPSRMEISKAR 600
            NE NDGECLHP+    +GKKRK+V+ + H+VENGEES P  EKRKKRKFPSR EISKAR
Sbjct: 541 SNETNDGECLHPQAN--DGKKRKKVVSEKHEVENGEESKPGVEKRKKRKFPSREEISKAR 600

Query: 601 EEKRQALREKKAGGKK 613
           EEKRQALREKKAGG K
Sbjct: 601 EEKRQALREKKAGGSK 609

BLAST of MC11g1071.1 vs. ExPASy TrEMBL
Match: A0A6J1FCL8 (probable 28S rRNA (Cytosine(4447)-C(5))-methyltransferase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442793 PE=3 SV=1)

HSP 1 Score: 965 bits (2494), Expect = 0.0
Identity = 527/615 (85.69%), Postives = 566/615 (92.03%), Query Frame = 0

Query: 1   MAAAKFNKKKGVKRNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVLD-D 60
           MAAAKFNKKK VKR+D P K KKK+ELFT KRS+EE++ DS++SDG+   EVENGVLD D
Sbjct: 1   MAAAKFNKKKVVKRDDLPLKGKKKDELFTRKRSQEEDD-DSEISDGMDMEEVENGVLDGD 60

Query: 61  DDGFGSGSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKSRAIDEER 120
           ++G  SGSEVSSDGDDPLADDFLQGSD+E    +GSGS SGSDSDED I NKSR IDEER
Sbjct: 61  EEGSESGSEVSSDGDDPLADDFLQGSDDEE---NGSGSDSGSDSDED-IENKSRIIDEER 120

Query: 121 ARVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREIVRVLTNFKAL 180
           AR EK+A DEM+LNIREESDEFRLP KEELEEE L PPDLSNLQRRIREIVRVLTNFKAL
Sbjct: 121 AREEKDAADEMQLNIREESDEFRLPTKEELEEEALGPPDLSNLQRRIREIVRVLTNFKAL 180

Query: 181 RQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKPRPICLRTNT 240
           RQEGATRKDYVEQLKKD+ SYYGYNEFL GVL+E+FPV+ELMELIEAFEKPRPICLRTNT
Sbjct: 181 RQEGATRKDYVEQLKKDLCSYYGYNEFLIGVLVEMFPVVELMELIEAFEKPRPICLRTNT 240

Query: 241 LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP 300
           LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP
Sbjct: 241 LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP 300

Query: 301 VMALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRMGVTN 360
           VMALAPQEKERIIDMAAAPGGKTTYIAALMKNSG+IFANEMKAPRLKSLTANL RMGVTN
Sbjct: 301 VMALAPQEKERIIDMAAAPGGKTTYIAALMKNSGLIFANEMKAPRLKSLTANLQRMGVTN 360

Query: 361 TVVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQKCSKLQKEL 420
           TVVC+YDGKELPKV+G NTADRVLLDAPCSGSGVVSKDESVKTSKS+EEIQKC++LQK+L
Sbjct: 361 TVVCNYDGKELPKVLGKNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQL 420

Query: 421 ILAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGLDFGRPGFIRF 480
           ILAAIDM D+NSKSGGYVVYSTCSI+VDENEAV+DYALKKRDVKL PCGLDFGRPGFIRF
Sbjct: 421 ILAAIDMVDSNSKSGGYVVYSTCSIMVDENEAVVDYALKKRDVKLAPCGLDFGRPGFIRF 480

Query: 481 REKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSDPLETAMEEDNESDKSE 540
           REKRFHPSLEKTRRFYPHVHNMDGFFVAKL+KMSNKIPTA+SS+ LE   EED+ES+KS 
Sbjct: 481 REKRFHPSLEKTRRFYPHVHNMDGFFVAKLQKMSNKIPTAQSSELLEETAEEDSESEKSG 540

Query: 541 NEENDGECLHPRKEQVEGKKRKEVLQNHDVENGEE-STPATEKRKKRKFPSRMEISKARE 600
           NE++DGECLHP+    +GKKRK+V +NHD ENGEE S P  EKRKKRKFPSR EISKARE
Sbjct: 541 NEKDDGECLHPKV--SDGKKRKKVSKNHDDENGEEESKPTVEKRKKRKFPSREEISKARE 600

Query: 601 EKRQALREKKAGGKK 613
           EKRQALREKK G KK
Sbjct: 601 EKRQALREKKTGSKK 608

BLAST of MC11g1071.1 vs. ExPASy TrEMBL
Match: A0A6J1F7G0 (probable 28S rRNA (Cytosine(4447)-C(5))-methyltransferase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442793 PE=3 SV=1)

HSP 1 Score: 961 bits (2483), Expect = 0.0
Identity = 525/615 (85.37%), Postives = 563/615 (91.54%), Query Frame = 0

Query: 1   MAAAKFNKKKGVKRNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVLD-D 60
           MAAAKFNKKK VKR+D P K KKK+ELFT KRS+EE++ DS++SDG+   EVENGVLD D
Sbjct: 1   MAAAKFNKKKVVKRDDLPLKGKKKDELFTRKRSQEEDD-DSEISDGMDMEEVENGVLDGD 60

Query: 61  DDGFGSGSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKSRAIDEER 120
           ++G  SGSEVSSDGDDPLADDFLQGSD+E       GS SGSDSDED I NKSR IDEER
Sbjct: 61  EEGSESGSEVSSDGDDPLADDFLQGSDDE-------GSDSGSDSDED-IENKSRIIDEER 120

Query: 121 ARVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREIVRVLTNFKAL 180
           AR EK+A DEM+LNIREESDEFRLP KEELEEE L PPDLSNLQRRIREIVRVLTNFKAL
Sbjct: 121 AREEKDAADEMQLNIREESDEFRLPTKEELEEEALGPPDLSNLQRRIREIVRVLTNFKAL 180

Query: 181 RQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKPRPICLRTNT 240
           RQEGATRKDYVEQLKKD+ SYYGYNEFL GVL+E+FPV+ELMELIEAFEKPRPICLRTNT
Sbjct: 181 RQEGATRKDYVEQLKKDLCSYYGYNEFLIGVLVEMFPVVELMELIEAFEKPRPICLRTNT 240

Query: 241 LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP 300
           LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP
Sbjct: 241 LKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYMLQGASSFLP 300

Query: 301 VMALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTANLHRMGVTN 360
           VMALAPQEKERIIDMAAAPGGKTTYIAALMKNSG+IFANEMKAPRLKSLTANL RMGVTN
Sbjct: 301 VMALAPQEKERIIDMAAAPGGKTTYIAALMKNSGLIFANEMKAPRLKSLTANLQRMGVTN 360

Query: 361 TVVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQKCSKLQKEL 420
           TVVC+YDGKELPKV+G NTADRVLLDAPCSGSGVVSKDESVKTSKS+EEIQKC++LQK+L
Sbjct: 361 TVVCNYDGKELPKVLGKNTADRVLLDAPCSGSGVVSKDESVKTSKSLEEIQKCAQLQKQL 420

Query: 421 ILAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGLDFGRPGFIRF 480
           ILAAIDM D+NSKSGGYVVYSTCSI+VDENEAV+DYALKKRDVKL PCGLDFGRPGFIRF
Sbjct: 421 ILAAIDMVDSNSKSGGYVVYSTCSIMVDENEAVVDYALKKRDVKLAPCGLDFGRPGFIRF 480

Query: 481 REKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSDPLETAMEEDNESDKSE 540
           REKRFHPSLEKTRRFYPHVHNMDGFFVAKL+KMSNKIPTA+SS+ LE   EED+ES+KS 
Sbjct: 481 REKRFHPSLEKTRRFYPHVHNMDGFFVAKLQKMSNKIPTAQSSELLEETAEEDSESEKSG 540

Query: 541 NEENDGECLHPRKEQVEGKKRKEVLQNHDVENGEE-STPATEKRKKRKFPSRMEISKARE 600
           NE++DGECLHP+    +GKKRK+V +NHD ENGEE S P  EKRKKRKFPSR EISKARE
Sbjct: 541 NEKDDGECLHPKV--SDGKKRKKVSKNHDDENGEEESKPTVEKRKKRKFPSREEISKARE 600

Query: 601 EKRQALREKKAGGKK 613
           EKRQALREKK G KK
Sbjct: 601 EKRQALREKKTGSKK 604

BLAST of MC11g1071.1 vs. TAIR 10
Match: AT4G26600.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 664.1 bits (1712), Expect = 1.1e-190
Identity = 379/641 (59.13%), Postives = 468/641 (73.01%), Query Frame = 0

Query: 9   KKGVKRNDPPSKTK----------KKEELFTAKRSKEEEENDSDL-----SDGLGDAEVE 68
           KK  K   PP K +          KK E+ T +  +EEE   SD      SD   D + E
Sbjct: 29  KKAAKTQKPPLKKQRKCISEKKPLKKPEVSTDEEEEEEENEQSDEGSESGSDLFSDGDEE 88

Query: 69  -NGVLDDDDGFGSGSEVSSDGDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKS 128
            N   DDDD      +   +  +PLA+DFL GSD E  +    GS   SDS    +  KS
Sbjct: 89  GNNDSDDDDDDDDDDDDDDEDAEPLAEDFLDGSDNEEVT---MGSDLDSDSGGSKLERKS 148

Query: 129 RAIDEERARVEKEAEDEMKLNIREESDEFRLPIKEELEEEVLRPPDLSNLQRRIREIVRV 188
           RAID +R +  ++A+DE K+NI+E+ DEF+LP ++ELEEE  RPPDL +LQ RIREIVR+
Sbjct: 149 RAIDRKRKKEVQDADDEFKMNIKEKPDEFQLPTQKELEEEARRPPDLPSLQMRIREIVRI 208

Query: 189 LTNFKALRQEG--ATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIEAFEKP 248
           L+NFK L+ +G    R DYV QLK D+ SYYGYNEFL G L+E+FPV+ELMELIEAFEK 
Sbjct: 209 LSNFKDLKPKGDKHERNDYVGQLKADLSSYYGYNEFLIGTLIEMFPVVELMELIEAFEKK 268

Query: 249 RPICLRTNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYMAGFYM 308
           RP  +RTNTLKTRRRDLAD+L+NRGVNLDPLSKWSKVGL+VYDSQVPIGATPEY+AGFYM
Sbjct: 269 RPTSIRTNTLKTRRRDLADILLNRGVNLDPLSKWSKVGLIVYDSQVPIGATPEYLAGFYM 328

Query: 309 LQGASSFLPVMALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLKSLTA 368
           LQ ASSFLPVMALAP+EKER++DMAAAPGGKTTY+AALMKN+GII+ANEMK PRLKSL+A
Sbjct: 329 LQSASSFLPVMALAPREKERVVDMAAAPGGKTTYVAALMKNTGIIYANEMKVPRLKSLSA 388

Query: 369 NLHRMGVTNTVVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQ 428
           NLHRMGVTNT+VC+YDG+EL KV+G ++ DRVLLDAPCSG+GV+SKDESVKTSKS ++I+
Sbjct: 389 NLHRMGVTNTIVCNYDGRELTKVLGQSSVDRVLLDAPCSGTGVISKDESVKTSKSADDIK 448

Query: 429 KCSKLQKELILAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLVPCGLD 488
           K + LQK+LIL AID+ DANSK+GGY+VYSTCS+++ ENEAVIDYALK RDVKLVPCGLD
Sbjct: 449 KFAHLQKQLILGAIDLVDANSKTGGYIVYSTCSVMIPENEAVIDYALKNRDVKLVPCGLD 508

Query: 489 FGRPGFIRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSDPLET--- 548
           FGRPGF  FRE RFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSN +  + + +P  T   
Sbjct: 509 FGRPGFSSFREHRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNAMQPSGNDEPAVTMEQ 568

Query: 549 --AMEEDNESDKSE--------------------NEENDGECLHPRKEQVEGKKRKEVLQ 607
                 D++ +K+E                     +E+  +  +PR +++   KR    +
Sbjct: 569 AQVSSSDDDDEKAEAIEELEKPPVASGQPKRESNTKEDTNKRKNPRSKEIHKGKRN---K 628

BLAST of MC11g1071.1 vs. TAIR 10
Match: AT5G55920.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 654.1 bits (1686), Expect = 1.1e-187
Identity = 382/642 (59.50%), Postives = 470/642 (73.21%), Query Frame = 0

Query: 9   KKGVK-RNDPPSKTKKKEELFTAKRSKEEEENDSDLSDGLGDAEVENGVL---------D 68
           KKG K +N PP K +KKE +        E  +DSD  D + D   E+ +          +
Sbjct: 39  KKGAKSQNKPPLKKQKKEVVEEEPLEDYEVTDDSDEDDEVSDGSDEDDISPAVESEEIDE 98

Query: 69  DDDGFGSGSEVSSD-----GDDPLADDFLQGSDEENGSGSGSGSVSGSDSDEDDIVNKSR 128
            DDG    +++ SD      ++ L DDFL+GS +E+  GS     S +DSD+DDIV KS 
Sbjct: 99  SDDGENGSNQLFSDDEEENDEETLGDDFLEGSGDEDEEGSLDAD-SDADSDDDDIVAKSD 158

Query: 129 AIDEERARVEKEAEDEMKLNIRE--------ESDEFRLPIKEELEEEVLRPPDLSNLQRR 188
           AID + A  +K+A  E++  I++        E D FRLP +EELEEE   PPDL  L+ R
Sbjct: 159 AIDRDLAMQKKDAAAELEDFIKQDDVHDEEPEHDAFRLPTEEELEEEARGPPDLPLLKTR 218

Query: 189 IREIVRVLTNFKALRQEGATRKDYVEQLKKDIGSYYGYNEFLTGVLMELFPVIELMELIE 248
           I EIVR L NFKA R +  TRK  VEQLK D+GSYYGYN FL G L+E+FP  ELMELIE
Sbjct: 219 IEEIVRALKNFKAFRPKDTTRKACVEQLKADLGSYYGYNSFLIGTLVEMFPPGELMELIE 278

Query: 249 AFEKPRPICLRTNTLKTRRRDLADVLINRGVNLDPLSKWSKVGLVVYDSQVPIGATPEYM 308
           AFEK RP  +RTNTLKTRRRDLADVL+NRGVNLDPLSKWSKVGLV+YDSQVPIGATPEY+
Sbjct: 279 AFEKQRPTSIRTNTLKTRRRDLADVLLNRGVNLDPLSKWSKVGLVIYDSQVPIGATPEYL 338

Query: 309 AGFYMLQGASSFLPVMALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRL 368
           AG+YMLQGASSFLPVMALAP+E ERI+D+AAAPGGKTTYIAALMKN+G+I+ANEMK PRL
Sbjct: 339 AGYYMLQGASSFLPVMALAPRENERIVDVAAAPGGKTTYIAALMKNTGLIYANEMKVPRL 398

Query: 369 KSLTANLHRMGVTNTVVCSYDGKELPKVMGTNTADRVLLDAPCSGSGVVSKDESVKTSKS 428
           KSLTANLHRMGVTNT+VC+YDG+ELPKV+G NT DRVLLDAPCSG+G++SKDESVK +K+
Sbjct: 399 KSLTANLHRMGVTNTIVCNYDGRELPKVLGQNTVDRVLLDAPCSGTGIISKDESVKITKT 458

Query: 429 VEEIQKCSKLQKELILAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDVKLV 488
           ++EI+K + LQK+L+LAAIDM DANSK+GGY+VYSTCSI+V ENEAVIDYALKKRDVKLV
Sbjct: 459 MDEIKKFAHLQKQLLLAAIDMVDANSKTGGYIVYSTCSIMVTENEAVIDYALKKRDVKLV 518

Query: 489 PCGLDFGRPGFIRFREKRFHPSLEKTRRFYPHVHNMDGFFVAKLKKMSNKIPTAESSD-- 548
            CGLDFGR GF RFRE RF PSL+KTRRFYPHVHNMDGFFVAKLKKMSN   ++E  D  
Sbjct: 519 TCGLDFGRKGFTRFREHRFQPSLDKTRRFYPHVHNMDGFFVAKLKKMSNVKQSSEEGDDD 578

Query: 549 ------PLETAMEEDNESDKSENEENDGECLHPRKEQVEGKKRKEVLQNHDVENG----- 608
                   E + ++D+E++  E  E     +   KE+ E K ++++ ++ + + G     
Sbjct: 579 AVETVEQAEVSSDDDDEAEAIEETEKPSVPVRQPKERKEKKNKEKLAKSKEDKRGKKDKK 638

BLAST of MC11g1071.1 vs. TAIR 10
Match: AT3G13180.1 (NOL1/NOP2/sun family protein / antitermination NusB domain-containing protein )

HSP 1 Score: 110.5 bits (275), Expect = 4.7e-24
Identity = 83/231 (35.93%), Postives = 122/231 (52.81%), Query Frame = 0

Query: 287 GFYMLQGASSFLPVMALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPRLK 346
           G   +Q  S+ L V  + PQ  ERI+D  AAPGGKT ++A+ +K  G+I+A ++   RL+
Sbjct: 310 GICSVQDESAGLIVSVVKPQPGERIMDACAAPGGKTLFMASCLKGQGMIYAMDVNEGRLR 369

Query: 347 SL--TANLHRMGVTNTVVCSYDGKELPKVMGTNTA--DRVLLDAPCSGSGVVSKDESVKT 406
            L  TA  H++    T + S    +L     TN    D+VLLDAPCSG GV+SK   ++ 
Sbjct: 370 ILGETAKSHQVDGLITTIHS----DLRVFAETNEVQYDKVLLDAPCSGLGVLSKRADLRW 429

Query: 407 SKSVEEIQKCSKLQKELILAAIDMADANSKSGGYVVYSTCSIIVDENEAVIDYALKKRDV 466
           ++ +E++ + +KLQ EL    +D A    K GG +VYSTCSI  +ENE  ++  L +   
Sbjct: 430 NRKLEDMLELTKLQDEL----LDSASKLVKHGGVLVYSTCSIDPEENEGRVEAFLLR--- 489

Query: 467 KLVPCGLDFGRPGFIRFREKRFHPSLEKTRRFY----PHVHNMDGFFVAKL 510
                      P F       F PS   T + +    P  H++DG F A+L
Sbjct: 490 ----------HPEFTIDPVTSFVPSSFVTSQGFFLSNPVKHSLDGAFAARL 519

BLAST of MC11g1071.1 vs. TAIR 10
Match: AT1G06560.1 (NOL1/NOP2/sun family protein )

HSP 1 Score: 72.8 bits (177), Expect = 1.1e-12
Identity = 82/325 (25.23%), Postives = 119/325 (36.62%), Query Frame = 0

Query: 285 MAGFYMLQGASSFLPVMALAPQEKERIIDMAAAPGGKTTYIAALMKNSGIIFANEMKAPR 344
           + G   LQ   S +   AL PQ+ ERI+DM AAPGGKTT IA LM + G I A +    +
Sbjct: 274 LEGEIFLQNLPSIIVAHALDPQKGERILDMCAAPGGKTTAIAILMNDEGEIVAADRSHNK 333

Query: 345 LKSLTANLHRMGVTNTVVCSYDGKE---LPKVMG-------------------------- 404
           +  +      MG T    C  D  +   LP  +                           
Sbjct: 334 VLVVQNLSAEMGFTCITTCKLDALKSVCLPTTLNESTILINGDNSSSMTSHSELSSNEEM 393

Query: 405 ------------------------------------------------------------ 464
                                                                       
Sbjct: 394 TSVTSRRSEADKSCEKNDSTEQPNGGDNVSQAYIRKNKGRLKNGRGRTQCQGGRAGKSQG 453

Query: 465 --TNTADRVLLDAPCSGSGVVSKDESVKTSKSVEEIQKCSKLQKELILAAIDMADANSKS 509
              N+ DRVLLDAPCS  G+  +       ++V  ++     Q++++  A+ +     + 
Sbjct: 454 FPPNSFDRVLLDAPCSALGL--RPRLFAGLETVVSLRNHGWYQRKMLDQAVQLV----RV 513

BLAST of MC11g1071.1 vs. TAIR 10
Match: AT4G17590.1 (CONTAINS InterPro DOMAIN/s: Bacterial Fmu (Sun)/eukaryotic nucleolar NOL1/Nop2p (InterPro:IPR001678); BEST Arabidopsis thaliana protein match is: S-adenosyl-L-methionine-dependent methyltransferases superfamily protein (TAIR:AT4G26600.1); Has 459 Blast hits to 368 proteins in 186 species: Archae - 2; Bacteria - 0; Metazoa - 137; Fungi - 144; Plants - 100; Viruses - 0; Other Eukaryotes - 76 (source: NCBI BLink). )

HSP 1 Score: 68.2 bits (165), Expect = 2.7e-11
Identity = 44/98 (44.90%), Postives = 64/98 (65.31%), Query Frame = 0

Query: 333 GIIFANEMKAPRLKSLTANLHRMGVTNTVVCSYD-GKELPKVMGTNTADRVLLDAPCSGS 392
           GIIFAN      L SL ANLHRMG+TNTVV +Y+   +L +V   N+ D VL++AP + +
Sbjct: 55  GIIFANASTEHLLGSLYANLHRMGITNTVVSNYNINTKLSRVFHINSKDMVLVNAPSTRT 114

Query: 393 GVVSKDESVKTSKSVE-EIQKCSKLQKELILAAIDMAD 429
           G++S+  S+K S + E +IQ+   LQK  I+  + M+D
Sbjct: 115 GLISEFGSIKMSINEEADIQRFGVLQK--IVEGVHMSD 150