MC11g1014.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC11g1014.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	MBOAT (membrane bound O-acyl transferase) family protein
Location	MC11: 8668082 .. 8669385 (-)
Sequence length	1115
RNA-Seq Expression	MC11g1014.1
Synteny	MC11g1014.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGGACGCCTCCACTCGCATGTCTTCATATGAACAAATTACAATCACATTGTTTGTACTAACTACAAAGTGGACCGTATTCATAGTGTCCGAGAATAAGACGTTTACCTATATTCCTATACTATATACCATTTAGACGGTTAACCTCTATATTTTTAGGCTACATTATCTTATCATTATGTTTGACATTATTATGTTTTTAAATAAAAATTCCATAAAATAAACACTCCTTGCCATTTTGAAAACTATATATATATATTAATATGATTTCAATTTGATCTACAATCTTCCTCGGTCCCTCTCCAGTCTCCACACGTCCCATTTCCACAGTTAAATTGCAGAGACGAACCACCACCTACCTCCGCCGTGGACTCCGCCGCCGGCAACCAAAATGGACGGCGAACTCATCAGCTTCACCAAGGTATGGACCATCGCCATTGCTTTCATTTCTTACTCTTACTTTGTAGCTTCCAAAATCCGTAACGGAATCCTAAGGCTTGCTGCTCTCCTCCCCGTTCTCGCCGTCTTCCTCCTCCTCCCGCTCAATCTCCGCTCCTTCCATCTCTGCGGTTCCACCGGTTTCCTCCTCGCCTGGCTCGCCAATTTCAAGCTCCTCCTCTTCGCCTTCCATCGCGGTCCTCTCGCTTCCTCCTCCGCCTCGGCGCTCCCGAGTTTCCTCCACTTCGCCGCCGCTCTCTGCCTCCCGATCAAGATCCAAAACCAAAAACCTAACAAACCTCGCCGCAATCGCAGAATCGGAGACTCGCCGCTCGTTTTCGCTTCCAAAATCTTGCTTCTAGCGACTGTGATTCGTCTCTACGAGTACCGGAATCGGATGGATCCGACGATCGTTCTCGTTCTGTACTGCCTCCATTTGTACCTCGGCGTCGAGATCGTCCTCGCTCTGTGTTATCTTCCGGCGAGGGCCATCTTCGGAGCTCCGATCGAGCCGCAGTTCAACGAGCCTTATCTCTCCACCTCGCTTCACGACTTCTGGAGCCGTCGATGGAATCTCATAGTACCAAGTATTCTACGCCCGGCCGTATACGATCCCACCCGCTCTATAACTTCCTACTTTTTTGGGCCGTTGTGGGCCCATCTTCTGGCCATCGTCGCGACCTTCTCTGTCTCCGGGTTGATGCACGAGGTCATGTGTGTATATATATATATATATATATATTACTTTTTTAGCTTTTAAAAAATATTTGATTATGTTTTTAGTTATGATTAATTATGTTTTTAGTTTTTTTTTTTCTCTTTTGAGTACAATTATGGTGTTAGCTAGTTTTAAAGTTTATAGCTTTT

mRNA sequence

CGGGACGCCTCCACTCGCATGTCTTCATATGAACAAATTACAATCACATTGTTTGTACTAACTACAAAGTGGACCGTATTCATAGTGTCCGAGAATAAGACGTTTACCTATATTCCTATACTATATACCATTTAGACGGTTAACCTCTATATTTTTAGGCTACATTATCTTATCATTATGTTTGACATTATTATGTTTTTAAATAAAAATTCCATAAAATAAACACTCCTTGCCATTTTGAAAACTATATATATATATTAATATGATTTCAATTTGATCTACAATCTTCCTCGGTCCCTCTCCAGTCTCCACACGTCCCATTTCCACAGTTAAATTGCAGAGACGAACCACCACCTACCTCCGCCGTGGACTCCGCCGCCGGCAACCAAAATGGACGGCGAACTCATCAGCTTCACCAAGCTCCTCCTCTTCGCCTTCCATCGCGGTCCTCTCGCTTCCTCCTCCGCCTCGGCGCTCCCGAGTTTCCTCCACTTCGCCGCCGCTCTCTGCCTCCCGATCAAGATCCAAAACCAAAAACCTAACAAACCTCGCCGCAATCGCAGAATCGGAGACTCGCCGCTCGTTTTCGCTTCCAAAATCTTGCTTCTAGCGACTGTGATTCGTCTCTACGAGTACCGGAATCGGATGGATCCGACGATCGTTCTCGTTCTGTACTGCCTCCATTTGTACCTCGGCGTCGAGATCGTCCTCGCTCTGTGTTATCTTCCGGCGAGGGCCATCTTCGGAGCTCCGATCGAGCCGCAGTTCAACGAGCCTTATCTCTCCACCTCGCTTCACGACTTCTGGAGCCGTCGATGGAATCTCATAGTACCAAGTATTCTACGCCCGGCCGTATACGATCCCACCCGCTCTATAACTTCCTACTTTTTTGGGCCGTTGTGGGCCCATCTTCTGGCCATCGTCGCGACCTTCTCTGTCTCCGGGTTGATGCACGAGGTCATGTGTGTATATATATATATATATATATATTACTTTTTTAGCTTTTAAAAAATATTTGATTATGTTTTTAGTTATGATTAATTATGTTTTTAGTTTTTTTTTTTCTCTTTTGAGTACAATTATGGTGTTAGCTAGTTTTAAAGTTTATAGCTTTT

Coding sequence (CDS)

ATGGACGGCGAACTCATCAGCTTCACCAAGCTCCTCCTCTTCGCCTTCCATCGCGGTCCTCTCGCTTCCTCCTCCGCCTCGGCGCTCCCGAGTTTCCTCCACTTCGCCGCCGCTCTCTGCCTCCCGATCAAGATCCAAAACCAAAAACCTAACAAACCTCGCCGCAATCGCAGAATCGGAGACTCGCCGCTCGTTTTCGCTTCCAAAATCTTGCTTCTAGCGACTGTGATTCGTCTCTACGAGTACCGGAATCGGATGGATCCGACGATCGTTCTCGTTCTGTACTGCCTCCATTTGTACCTCGGCGTCGAGATCGTCCTCGCTCTGTGTTATCTTCCGGCGAGGGCCATCTTCGGAGCTCCGATCGAGCCGCAGTTCAACGAGCCTTATCTCTCCACCTCGCTTCACGACTTCTGGAGCCGTCGATGGAATCTCATAGTACCAAGTATTCTACGCCCGGCCGTATACGATCCCACCCGCTCTATAACTTCCTACTTTTTTGGGCCGTTGTGGGCCCATCTTCTGGCCATCGTCGCGACCTTCTCTGTCTCCGGGTTGATGCACGAGGTCATGTGTGTATATATATATATATATATATATTACTTTTTTAGCTTTTAA

Protein sequence

MDGELISFTKLLLFAFHRGPLASSSASALPSFLHFAAALCLPIKIQNQKPNKPRRNRRIGDSPLVFASKILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQFNEPYLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGLMHEVMCVYIYIYIYYFFSF

Homology

[Close]

BLAST of MC11g1014.1 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 4.2e-38
Identity = 86/188 (45.74%), Postives = 120/188 (63.83%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASS-SASALPSFLHFAAALCLPIKIQ-NQKPNKPRRNRRIGDSPLVFA 69
           KLLLFA  RGPL+S+    +LP FL   A  CLPIKIQ + KP K   +    + PL++ 
Sbjct: 69  KLLLFALGRGPLSSNHKPLSLPIFL---AVSCLPIKIQLSPKPTKTHSHEGSTEGPLIYT 128

Query: 70  SKILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQFN 129
            K + +  +I+ YEY  ++   +VL LY +H+Y  +EI+LA      RA+    +EPQFN
Sbjct: 129 IKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQFN 188

Query: 130 EPYLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGLM 189
           +PYL+TSL DFW RRWNL+V  ILRP VY+P+  + S   GP ++ +LA   TF VSG+M
Sbjct: 189 KPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFGTFVVSGIM 248

Query: 190 HEVMCVYI 196
           HE++  Y+
Sbjct: 249 HELIFFYM 252

[Close]

BLAST of MC11g1014.1 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.0e-36
Identity = 83/190 (43.68%), Postives = 113/190 (59.47%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASSSASALPSFLHFAAALCLPIKIQNQKPNKPRRNRRI----GDSPLV 69
           KLLLFAFH GPL++ S     S LHF +   LPIK ++   N   +N+R         ++
Sbjct: 76  KLLLFAFHLGPLSNPSL----SLLHFISTTLLPIKFRDDPSNDHEKNKRTLSFEWRKVVL 135

Query: 70  FASKILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQ 129
           F +K++  A ++++YE+R  +   ++ VLYC H YLG EI LA   + ARA  G  + PQ
Sbjct: 136 FVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVIARATLGLDLYPQ 195

Query: 130 FNEPYLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSG 189
           FNEPYL+TSL DFW RRWNL+V  IL    Y P R + S +    W    A++  F+VSG
Sbjct: 196 FNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEVAGAMLVAFTVSG 255

Query: 190 LMHEVMCVYI 196
           LMHEV   Y+
Sbjct: 256 LMHEVFFFYL 261

[Close]

BLAST of MC11g1014.1 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.3e-34
Identity = 81/186 (43.55%), Postives = 110/186 (59.14%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASSSASALPSFLHFAAALCLPIKIQNQKPNKPRRNRRIGDSPLVFASK 69
           KL+LF+F +GPL         +F  F    C PIK+Q      P+    +    LVFA K
Sbjct: 73  KLILFSFDKGPLIPIPT----NFPRFFCFTCFPIKVQ----QNPKSQNHL--PKLVFAIK 132

Query: 70  ILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQFNEP 129
           + + A ++ LY YR  + PTI+L LY +HLYL +EI+L    +      G  +EPQ N+P
Sbjct: 133 LAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLGCDLEPQSNKP 192

Query: 130 YLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGLMHE 189
           YL+TSL DFW RRWNL+VP+ILRPAVY P R ++       WA    I+A F VSGL+HE
Sbjct: 193 YLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAAFIVSGLVHE 248

Query: 190 VMCVYI 196
           ++  Y+
Sbjct: 253 LLFFYL 248

[Close]

BLAST of MC11g1014.1 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.4e-33
Identity = 84/189 (44.44%), Postives = 110/189 (58.20%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASSSASALPSFLHFAAALCLPIKIQNQKPNKPRRNRRIGDSPL---VF 69
           KLLLFAF  GPL        P    F    CLPIKI+ Q P+ P     +   P+   V 
Sbjct: 73  KLLLFAFDEGPLFPLP----PKLSRFICFACLPIKIR-QDPS-PNAIPNLHPKPMPKWVL 132

Query: 70  ASKILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQF 129
           A KIL+L  ++ +YEYR+ +   +VL LYCLH+YL VE+VL         + G  IEP F
Sbjct: 133 AVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLLGCNIEPVF 192

Query: 130 NEPYLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGL 189
           NEPYL+TSL DFWSRRWNL+V ++LR  V+ P +        P  A    ++A+F VSGL
Sbjct: 193 NEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVMASFFVSGL 252

Query: 190 MHEVMCVYI 196
           MHE++  Y+
Sbjct: 253 MHELLYFYM 255

[Close]

BLAST of MC11g1014.1 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.6e-32
Identity = 82/186 (44.09%), Postives = 109/186 (58.60%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASSSASALPSFL-HFAAALCLPIKIQNQKPNKPRRNRRIGDSPLVFAS 69
           KL+LF+F +GPL       +PS L  F    C PIK+Q Q P    +  + G     FA 
Sbjct: 73  KLILFSFDQGPL-----FPVPSNLSRFVCFTCFPIKLQ-QNPKPQNQMPKWG-----FAV 132

Query: 70  KILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQFNE 129
           K+     ++ +YEY+  M PT++LVLY LH+YL  EI+LA   +         +EP FNE
Sbjct: 133 KLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDLEPHFNE 192

Query: 130 PYLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGLMH 189
           PYLSTSL DFW RRWNL+VP+ILRPAVY P R +         A  L + A+F VSG++H
Sbjct: 193 PYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFLVSGVVH 247

Query: 190 EVMCVY 195
           E++  Y
Sbjct: 253 ELIFFY 247

[Close]

BLAST of MC11g1014.1 vs. NCBI nr
Match: XP_023525242.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 252 bits (643), Expect = 1.15e-79
Identity = 129/185 (69.73%), Postives = 149/185 (80.54%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASSSASALPSFLHFAAALCLPIKIQNQKPNKPRRNRRIGDSPLVFASK 69
           KLLLFAF  GPL S     LPS LHFAA LCLPIKI+ QK  +  +NRRI + P+V + K
Sbjct: 90  KLLLFAFDLGPLTSLP---LPSLLHFAAVLCLPIKIKKQKHPEESKNRRIVE-PIVLSVK 149

Query: 70  ILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQFNEP 129
           +LLLA +IRLY YRNRM+P ++ VLYCLH+Y+G+EIVLALC LPARA+ GAPIEPQFN+P
Sbjct: 150 VLLLAIIIRLYSYRNRMNPAMIFVLYCLHMYIGIEIVLALCSLPARAMIGAPIEPQFNKP 209

Query: 130 YLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGLMHE 189
           YLSTSL DFWSRRWNL+VPSILRPAVYDPTRSITS  F P WA  LA+V TF+VSG MHE
Sbjct: 210 YLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSITSRIFRPKWAQFLAMVVTFAVSGFMHE 269

Query: 190 VMCVY 194
           +M  Y
Sbjct: 270 LMYYY 270

[Close]

BLAST of MC11g1014.1 vs. NCBI nr
Match: KAG7037354.1 (putative long-chain-alcohol O-fatty-acyltransferase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 250 bits (639), Expect = 4.64e-79
Identity = 127/185 (68.65%), Postives = 149/185 (80.54%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASSSASALPSFLHFAAALCLPIKIQNQKPNKPRRNRRIGDSPLVFASK 69
           KLLLFAF  GPL S     LPS LHFAA LCLPIKI+ QK  +  +NRRI + P+V + K
Sbjct: 90  KLLLFAFDLGPLTSLP---LPSLLHFAAVLCLPIKIKKQKHPEESKNRRIVE-PIVLSVK 149

Query: 70  ILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQFNEP 129
           +LLLA +IRLY YRNR++P ++ VLYCLH+Y+G+EIVLALC LPARA+ GAPIEPQFN+P
Sbjct: 150 VLLLAIIIRLYSYRNRLNPAMIFVLYCLHMYIGIEIVLALCSLPARAMIGAPIEPQFNKP 209

Query: 130 YLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGLMHE 189
           YLSTSL DFWSRRWNL+VPSILRPAVYDPTRS+TS  F P WA  LA+V TF+VSG MHE
Sbjct: 210 YLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSVTSRIFRPKWAQFLAMVVTFTVSGFMHE 269

Query: 190 VMCVY 194
           +M  Y
Sbjct: 270 LMYYY 270

[Close]

BLAST of MC11g1014.1 vs. NCBI nr
Match: XP_022981522.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita maxima])

HSP 1 Score: 248 bits (633), Expect = 3.74e-78
Identity = 127/185 (68.65%), Postives = 149/185 (80.54%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASSSASALPSFLHFAAALCLPIKIQNQKPNKPRRNRRIGDSPLVFASK 69
           KLLLFAF  GPL   +   LPS LHFAA LCLPIKI+ QK  +  +NRRI + P+V + K
Sbjct: 90  KLLLFAFDLGPL---TYLPLPSLLHFAAVLCLPIKIKKQKHPEESKNRRIVE-PIVLSVK 149

Query: 70  ILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQFNEP 129
           ILLLA +IRLY YRNRM+P ++ VLYCLH+Y+G+EIVLALC LP+RA+ G PIEPQFN+P
Sbjct: 150 ILLLAIIIRLYSYRNRMNPAMIFVLYCLHMYIGIEIVLALCSLPSRAMIGEPIEPQFNKP 209

Query: 130 YLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGLMHE 189
           YLSTSL DFWSRRWNL+VPSILRPAVYDPTRS+TS  F P WA  LA+V TF+VSGLMHE
Sbjct: 210 YLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSLTSRIFRPKWAQFLAMVVTFAVSGLMHE 269

Query: 190 VMCVY 194
           +M  Y
Sbjct: 270 LMYYY 270

[Close]

BLAST of MC11g1014.1 vs. NCBI nr
Match: XP_038898664.1 (probable long-chain-alcohol O-fatty-acyltransferase 4 [Benincasa hispida])

HSP 1 Score: 243 bits (619), Expect = 5.01e-76
Identity = 130/185 (70.27%), Postives = 144/185 (77.84%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASSSASALPSFLHFAAALCLPIKIQNQKPNKPRRNRRIGDSPLVFASK 69
           KLLLFAF  GPL SS    LPS LHFA  LCLPIKI NQK       RR G+  +V + K
Sbjct: 95  KLLLFAFDLGPLFSSP---LPSLLHFATMLCLPIKINNQKSEI----RRTGNQ-IVLSFK 154

Query: 70  ILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQFNEP 129
           +LLLA VIRLY+YRNRMDP I  VLYCLHLY G+EIVLALC LPA+AIFGAPIEPQF+EP
Sbjct: 155 VLLLAIVIRLYDYRNRMDPAIASVLYCLHLYFGIEIVLALCSLPAKAIFGAPIEPQFDEP 214

Query: 130 YLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGLMHE 189
           YLSTSL DFW  RWNL+VPSILRPAVY+PTR ITS  FGP WA  LA+VATF+VSG MHE
Sbjct: 215 YLSTSLQDFWGHRWNLMVPSILRPAVYNPTRFITSRVFGPRWAQFLAMVATFAVSGFMHE 271

Query: 190 VMCVY 194
           ++  Y
Sbjct: 275 LIFYY 271

[Close]

BLAST of MC11g1014.1 vs. NCBI nr
Match: KAA0048143.1 (putative long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo var. makuwa] >TYK08112.1 putative long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 239 bits (610), Expect = 5.61e-75
Identity = 129/185 (69.73%), Postives = 145/185 (78.38%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASSSASALPSFLHFAAALCLPIKIQNQKPNKPRRNRRIGDSPLVFASK 69
           KLLLFAF  GPL SS    LPSFLHFAA LCLPIKI + K ++ RRN     +P+V   K
Sbjct: 74  KLLLFAFDLGPLFSSP---LPSFLHFAATLCLPIKISSHK-SEIRRN----GNPIVLCLK 133

Query: 70  ILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQFNEP 129
           +LLLA V+RLY+YRNRMDP IV VLY LHLY G+EIVLA+C LP +AIFGAP EPQF+EP
Sbjct: 134 VLLLAIVVRLYDYRNRMDPAIVSVLYFLHLYFGIEIVLAVCSLPGKAIFGAPFEPQFDEP 193

Query: 130 YLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGLMHE 189
           YLSTSL DFWSRRWNL+VPSILRPAVYDPTR ITS  FG  WA  L +VATF VSGLMHE
Sbjct: 194 YLSTSLQDFWSRRWNLMVPSILRPAVYDPTRIITSRAFGRRWAQFLGMVATFVVSGLMHE 250

Query: 190 VMCVY 194
           ++  Y
Sbjct: 254 LIFYY 250

[Close]

BLAST of MC11g1014.1 vs. ExPASy TrEMBL
Match: A0A6J1IU70 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=3661 GN=LOC111480612 PE=3 SV=1)

HSP 1 Score: 248 bits (633), Expect = 1.81e-78
Identity = 127/185 (68.65%), Postives = 149/185 (80.54%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASSSASALPSFLHFAAALCLPIKIQNQKPNKPRRNRRIGDSPLVFASK 69
           KLLLFAF  GPL   +   LPS LHFAA LCLPIKI+ QK  +  +NRRI + P+V + K
Sbjct: 90  KLLLFAFDLGPL---TYLPLPSLLHFAAVLCLPIKIKKQKHPEESKNRRIVE-PIVLSVK 149

Query: 70  ILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQFNEP 129
           ILLLA +IRLY YRNRM+P ++ VLYCLH+Y+G+EIVLALC LP+RA+ G PIEPQFN+P
Sbjct: 150 ILLLAIIIRLYSYRNRMNPAMIFVLYCLHMYIGIEIVLALCSLPSRAMIGEPIEPQFNKP 209

Query: 130 YLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGLMHE 189
           YLSTSL DFWSRRWNL+VPSILRPAVYDPTRS+TS  F P WA  LA+V TF+VSGLMHE
Sbjct: 210 YLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSLTSRIFRPKWAQFLAMVVTFAVSGLMHE 269

Query: 190 VMCVY 194
           +M  Y
Sbjct: 270 LMYYY 270

[Close]

BLAST of MC11g1014.1 vs. ExPASy TrEMBL
Match: A0A5A7TX12 (Putative long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G002780 PE=3 SV=1)

HSP 1 Score: 239 bits (610), Expect = 2.72e-75
Identity = 129/185 (69.73%), Postives = 145/185 (78.38%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASSSASALPSFLHFAAALCLPIKIQNQKPNKPRRNRRIGDSPLVFASK 69
           KLLLFAF  GPL SS    LPSFLHFAA LCLPIKI + K ++ RRN     +P+V   K
Sbjct: 74  KLLLFAFDLGPLFSSP---LPSFLHFAATLCLPIKISSHK-SEIRRN----GNPIVLCLK 133

Query: 70  ILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQFNEP 129
           +LLLA V+RLY+YRNRMDP IV VLY LHLY G+EIVLA+C LP +AIFGAP EPQF+EP
Sbjct: 134 VLLLAIVVRLYDYRNRMDPAIVSVLYFLHLYFGIEIVLAVCSLPGKAIFGAPFEPQFDEP 193

Query: 130 YLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGLMHE 189
           YLSTSL DFWSRRWNL+VPSILRPAVYDPTR ITS  FG  WA  L +VATF VSGLMHE
Sbjct: 194 YLSTSLQDFWSRRWNLMVPSILRPAVYDPTRIITSRAFGRRWAQFLGMVATFVVSGLMHE 250

Query: 190 VMCVY 194
           ++  Y
Sbjct: 254 LIFYY 250

[Close]

BLAST of MC11g1014.1 vs. ExPASy TrEMBL
Match: A0A1S3BIX3 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo OX=3656 GN=LOC103490376 PE=3 SV=1)

HSP 1 Score: 239 bits (610), Expect = 4.37e-75
Identity = 129/185 (69.73%), Postives = 145/185 (78.38%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASSSASALPSFLHFAAALCLPIKIQNQKPNKPRRNRRIGDSPLVFASK 69
           KLLLFAF  GPL SS    LPSFLHFAA LCLPIKI + K ++ RRN     +P+V   K
Sbjct: 90  KLLLFAFDLGPLFSSP---LPSFLHFAATLCLPIKISSHK-SEIRRN----GNPIVLCLK 149

Query: 70  ILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQFNEP 129
           +LLLA V+RLY+YRNRMDP IV VLY LHLY G+EIVLA+C LP +AIFGAP EPQF+EP
Sbjct: 150 VLLLAIVVRLYDYRNRMDPAIVSVLYFLHLYFGIEIVLAVCSLPGKAIFGAPFEPQFDEP 209

Query: 130 YLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGLMHE 189
           YLSTSL DFWSRRWNL+VPSILRPAVYDPTR ITS  FG  WA  L +VATF VSGLMHE
Sbjct: 210 YLSTSLQDFWSRRWNLMVPSILRPAVYDPTRIITSRAFGRRWAQFLGMVATFVVSGLMHE 266

Query: 190 VMCVY 194
           ++  Y
Sbjct: 270 LIFYY 266

[Close]

BLAST of MC11g1014.1 vs. ExPASy TrEMBL
Match: A0A2G5C793 (MBOAT_2 domain-containing protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_08300099v1 PE=3 SV=1)

HSP 1 Score: 194 bits (492), Expect = 2.59e-57
Identity = 104/197 (52.79%), Postives = 136/197 (69.04%), Query Frame = 0


Query: 1   MDGELISFT---KLLLFAFHRGPLASSSASALPSFLHFAAALCLPIKIQNQKPNKPRRNR 60
           M G  IS+    KLLLFAF +GPL+S+S S+LP F+  A   CLPIKIQ+  P+      
Sbjct: 61  MTGFTISWIANFKLLLFAFDQGPLSSTSLSSLPLFISIA---CLPIKIQHS-PSPQITKN 120

Query: 61  RIGDSPLVFASKILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAI 120
            I  S L++  K+LLLA +IR+Y++R  +DPT++L LY LH+YL ++I+LA+    AR++
Sbjct: 121 LISKSTLIYVVKVLLLAVLIRIYDHREHIDPTLLLCLYSLHIYLSLDIMLAIAATSARSL 180

Query: 121 FGAPIEPQFNEPYLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAI 180
            G  +EPQFNEPY STSL DFW RRWNL++ +ILRP VYDPTR I S   G  WA +  I
Sbjct: 181 LGLELEPQFNEPYRSTSLQDFWGRRWNLMITTILRPTVYDPTRRIFSRLIGKNWASIWGI 240

Query: 181 VATFSVSGLMHEVMCVY 194
           V TF VSGLMHE++  Y
Sbjct: 241 VTTFVVSGLMHELLFFY 253

[Close]

BLAST of MC11g1014.1 vs. ExPASy TrEMBL
Match: A0A7J7L895 (MBOAT_2 domain-containing protein OS=Kingdonia uniflora OX=39325 GN=GIB67_039119 PE=3 SV=1)

HSP 1 Score: 190 bits (482), Expect = 2.88e-55
Identity = 104/190 (54.74%), Postives = 131/190 (68.95%), Query Frame = 0


Query: 5   LISFTKLLLFAFHRGPLASSSASALPSFLHFAAALCLPIKIQNQKPNKPRRNRRIGDSPL 64
           L SF KLLLFAF++GPL+SS    LPS LHF +  CLPIK+Q+Q  +     + +  SPL
Sbjct: 69  LCSF-KLLLFAFNQGPLSSSP---LPSLLHFISIACLPIKLQSQTSSNQITQKGL-KSPL 128

Query: 65  VFASKILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEP 124
            +A K LLLA VI +Y+Y+  +    +L LYC H+Y   EI+LA+   PARA+FG  IEP
Sbjct: 129 NYAIKALLLAIVIHIYDYKPYLHQDAILALYCCHMYFSAEILLAMSTAPARAVFGFEIEP 188

Query: 125 QFNEPYLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVS 184
            FNEPYLSTSL DFW +RWNL+VPSILRP VY+P R I++   G   A LL +V TF VS
Sbjct: 189 HFNEPYLSTSLQDFWGKRWNLMVPSILRPTVYNPIRYISNGILGKQCAQLLGVVGTFVVS 248

Query: 185 GLMHEVMCVY 194
           GLMHE++  Y
Sbjct: 249 GLMHELIYFY 253

[Close]

BLAST of MC11g1014.1 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 159.5 bits (402), Expect = 3.0e-39
Identity = 86/188 (45.74%), Postives = 120/188 (63.83%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASS-SASALPSFLHFAAALCLPIKIQ-NQKPNKPRRNRRIGDSPLVFA 69
           KLLLFA  RGPL+S+    +LP FL   A  CLPIKIQ + KP K   +    + PL++ 
Sbjct: 69  KLLLFALGRGPLSSNHKPLSLPIFL---AVSCLPIKIQLSPKPTKTHSHEGSTEGPLIYT 128

Query: 70  SKILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQFN 129
            K + +  +I+ YEY  ++   +VL LY +H+Y  +EI+LA      RA+    +EPQFN
Sbjct: 129 IKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQFN 188

Query: 130 EPYLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGLM 189
           +PYL+TSL DFW RRWNL+V  ILRP VY+P+  + S   GP ++ +LA   TF VSG+M
Sbjct: 189 KPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFGTFVVSGIM 248

Query: 190 HEVMCVYI 196
           HE++  Y+
Sbjct: 249 HELIFFYM 252

[Close]

BLAST of MC11g1014.1 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 147.9 bits (372), Expect = 8.9e-36
Identity = 81/186 (43.55%), Postives = 110/186 (59.14%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASSSASALPSFLHFAAALCLPIKIQNQKPNKPRRNRRIGDSPLVFASK 69
           KL+LF+F +GPL         +F  F    C PIK+Q      P+    +    LVFA K
Sbjct: 73  KLILFSFDKGPLIPIPT----NFPRFFCFTCFPIKVQ----QNPKSQNHL--PKLVFAIK 132

Query: 70  ILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQFNEP 129
           + + A ++ LY YR  + PTI+L LY +HLYL +EI+L    +      G  +EPQ N+P
Sbjct: 133 LAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLGCDLEPQSNKP 192

Query: 130 YLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGLMHE 189
           YL+TSL DFW RRWNL+VP+ILRPAVY P R ++       WA    I+A F VSGL+HE
Sbjct: 193 YLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAAFIVSGLVHE 248

Query: 190 VMCVYI 196
           ++  Y+
Sbjct: 253 LLFFYL 248

[Close]

BLAST of MC11g1014.1 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 143.7 bits (361), Expect = 1.7e-34
Identity = 84/189 (44.44%), Postives = 110/189 (58.20%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASSSASALPSFLHFAAALCLPIKIQNQKPNKPRRNRRIGDSPL---VF 69
           KLLLFAF  GPL        P    F    CLPIKI+ Q P+ P     +   P+   V 
Sbjct: 73  KLLLFAFDEGPLFPLP----PKLSRFICFACLPIKIR-QDPS-PNAIPNLHPKPMPKWVL 132

Query: 70  ASKILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQF 129
           A KIL+L  ++ +YEYR+ +   +VL LYCLH+YL VE+VL         + G  IEP F
Sbjct: 133 AVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLLGCNIEPVF 192

Query: 130 NEPYLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGL 189
           NEPYL+TSL DFWSRRWNL+V ++LR  V+ P +        P  A    ++A+F VSGL
Sbjct: 193 NEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVMASFFVSGL 252

Query: 190 MHEVMCVYI 196
           MHE++  Y+
Sbjct: 253 MHELLYFYM 255

[Close]

BLAST of MC11g1014.1 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 140.2 bits (352), Expect = 1.9e-33
Identity = 82/186 (44.09%), Postives = 109/186 (58.60%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASSSASALPSFL-HFAAALCLPIKIQNQKPNKPRRNRRIGDSPLVFAS 69
           KL+LF+F +GPL       +PS L  F    C PIK+Q Q P    +  + G     FA 
Sbjct: 73  KLILFSFDQGPL-----FPVPSNLSRFVCFTCFPIKLQ-QNPKPQNQMPKWG-----FAV 132

Query: 70  KILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQFNE 129
           K+     ++ +YEY+  M PT++LVLY LH+YL  EI+LA   +         +EP FNE
Sbjct: 133 KLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDLEPHFNE 192

Query: 130 PYLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGLMH 189
           PYLSTSL DFW RRWNL+VP+ILRPAVY P R +         A  L + A+F VSG++H
Sbjct: 193 PYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFLVSGVVH 247

Query: 190 EVMCVY 195
           E++  Y
Sbjct: 253 ELIFFY 247

[Close]

BLAST of MC11g1014.1 vs. TAIR 10
Match: AT1G34520.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 137.9 bits (346), Expect = 9.2e-33
Identity = 76/186 (40.86%), Postives = 106/186 (56.99%), Query Frame = 0


Query: 10  KLLLFAFHRGPLASSSASALPSFLHFAAALCLPIKIQNQKPNKPRRNRRIGDSPLVFASK 69
           KL+LFAF +GPL    A    +   F    CLPIKIQ + PN      +      VF  K
Sbjct: 73  KLILFAFDKGPLLPLPA----NLFRFICFTCLPIKIQ-KNPNSQNHLPK-----WVFFCK 132

Query: 70  ILLLATVIRLYEYRNRMDPTIVLVLYCLHLYLGVEIVLALCYLPARAIFGAPIEPQFNEP 129
             +   ++ ++ Y++ + P +++ LY LHLYL ++++L +       I G  +EP FNEP
Sbjct: 133 AAIFGVLLNVHNYKSSLPPILLICLYPLHLYLVLDVLLTIVNALLTIILGCDLEPHFNEP 192

Query: 130 YLSTSLHDFWSRRWNLIVPSILRPAVYDPTRSITSYFFGPLWAHLLAIVATFSVSGLMHE 189
           YL+TSL DFW RRWNL+VP+I RP VY P RS+        WA  +    TF VSGL+HE
Sbjct: 193 YLATSLQDFWGRRWNLMVPAIFRPGVYHPMRSVCQPQMRSDWARFMGCWTTFFVSGLIHE 248

Query: 190 VMCVYI 196
           ++  YI
Sbjct: 253 LVYFYI 248

The following BLAST results are available for this feature:

BLAST of MC11g1014.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	4.2e-38	45.74	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9XGY6	1.0e-36	43.68	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9FJ76	1.3e-34	43.55	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]
Q9FJ72	2.4e-33	44.44	Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX...	[more]
Q9FJ75	2.6e-32	44.09	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]

BLAST of MC11g1014.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023525242.1	1.15e-79	69.73	probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita pepo subsp. pep...	[more]
KAG7037354.1	4.64e-79	68.65	putative long-chain-alcohol O-fatty-acyltransferase 4, partial [Cucurbita argyro...	[more]
XP_022981522.1	3.74e-78	68.65	probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita maxima]	[more]
XP_038898664.1	5.01e-76	70.27	probable long-chain-alcohol O-fatty-acyltransferase 4 [Benincasa hispida]	[more]
KAA0048143.1	5.61e-75	69.73	putative long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo var. makuwa]...	[more]

BLAST of MC11g1014.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IU70	1.81e-78	68.65	probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=366...	[more]
A0A5A7TX12	2.72e-75	69.73	Putative long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo var. makuw...	[more]
A0A1S3BIX3	4.37e-75	69.73	probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo OX=3656 GN...	[more]
A0A2G5C793	2.59e-57	52.79	MBOAT_2 domain-containing protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_08300...	[more]
A0A7J7L895	2.88e-55	54.74	MBOAT_2 domain-containing protein OS=Kingdonia uniflora OX=39325 GN=GIB67_039119...	[more]

BLAST of MC11g1014.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	3.0e-39	45.74	acyl-CoA sterol acyl transferase 1	[more]
AT5G55340.1	8.9e-36	43.55	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	1.7e-34	44.44	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55350.1	1.9e-33	44.09	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT1G34520.1	9.2e-33	40.86	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 123..197 e-value: 3.9E-14 score: 52.7
None	No IPR available	PANTHER	PTHR31595:SF38	MBOAT (MEMBRANE BOUND O-ACYL TRANSFERASE) FAMILY PROTEIN	coord: 7..195
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 7..195

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC11g1014	MC11g1014	gene

The following utr3 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g1014.1-utr3	MC11g1014.1-utr3-MC11:8668082..8668188	utr3

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g1014.1-cds	MC11g1014.1-cds-MC11:8668189..8668776	CDS
MC11g1014.1-cds	MC11g1014.1-cds-MC11:8668966..8668995	CDS

The following utr5 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g1014.1-utr5	MC11g1014.1-utr5-MC11:8668996..8669385	utr5

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC11g1014.1	MC11g1014.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity