MC11g1004.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC11g1004.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	GrpE protein homolog
Location	MC11: 8597683 .. 8600770 (+)
Sequence length	1680
RNA-Seq Expression	MC11g1004.1
Synteny	MC11g1004.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGAACCGCTTCAAACAAAAATAGCTCAAAACAACAGCCACTGTAGATCCCTGTTTCTCCGAGCAGCCGCCTCCGTTTTCCCCAAAATTTTCCAAAAAAGTTGGAAAGGGCTTGGAATTTCTGCAGCTTTGAAACAATCGATTTCGTTTTCCCAACTCAGCGTCCGCCATTTCAATCTCTTCTTCCCAATGTCCGTTTCGAGGGTTCTGTTTCGAGCTTCCACCAGGAGCCTGCGCCGGAGCTCGCCGATTCTCTCCAAGCAGTTTCGCGGTCTCGTTCATGAGACTTCCGGCAAGGTTGAGGTTCTAAAGCCGTGTTTTCAAAGGTTCTCTTCTCTGGGGTTTTGTTAGGGTTTTAGCGATTTCATTTGAAAATGCTCTCTGATTCAGATTTTTGGTTTTCTTCGTTATCATATGCCCTTATGAAGCCTCTTCTTTCATTTGGTTGCGAAATCTTTGGTCTTGAGCTACCATTACTCTTTGGGTGATCATTGCTTCCTGTTCAGTTCGGTTTCAAAATAAGAACCAAGAATTTTCATTATAGAAAAATTGAGAGGTGATTCGATCTGGGTGTGGTTGAACATTATGAACTTGTGAATTTTAGCATGAATGTTGTTGATTTAGTGCTGCCTGTTTAACATGTTTTTGCATTTTTCATCATGTTTTAGCCTGCATTACCCTGCTTAGGTACTTTCATTCAAATTTCACCCTTAGCAGTTTATTTTTATGGTTTGAAGTTGAAGCTTGATCTTTCTCTTTTTACCTAGCTGGGCAGTTTAAGAAGCACGGACACGTCAGTTTGAGTAATGTGTCCGTGTACGACATGTGTCAAATACTCCGGCCCTCTAACACGTATTAGATACTGGTTAACACAATAAACCTGTTTAGACAATAGCCAATTGAATATGAGTCCAAGACTTATTGGCACGTATTGAATGCTCGCGCATAAAGAAAATACATCCAAACCGTAAGCATAATGCATAAGCTATAGACTTTAAATTTCCTTCATGCGTAAAAATAATATATTATAAAACATGTATATTTAATCAACATTCTCTTCGGTTTCCGTATAGAAAATAATATGTCTGTATTTGTATCCGTATCGTGTTGAGTCTATGTGTCGTGTCCATCTCCATACTTCTCAGTTGACAGTTGTAGGATACACGATTTCTGTTCCTGTTACTGATTAATATGCTTCATCTTCCAGTTTGCTCCAGTTCAGGTGTCTCTATTACATCATATAGTCTTCAATCCATTCCCCTTCCAAAAATTTGGAATATCTTCTACTACTTCCCATGAATCTTCTGAAAATAAAGAGGGGAGTGCCAAACAAAGTAAGAATTATTCTACAACTAAAGATCCTACAAAAGATGAGAATTCTGAAGTTTCTGACCAAGCAGGAGAATTGAATTCTACATCGAACTCCCATTCCACCAAATCGAAATCAAGACAATGGAGAAGACGCACTATACGAACTGCCTTTTCTGATTCGGATTCCGAGGGTTGTGAGCAACCTTCCATGGATGATCTGGTAAAATCACTGGCGCGGAAGGAAGAACTTTTGAAGTCTAAGCACAAAGAGATTAAGAAAATGCAAGACAAAGTCCTCCGTTCTTATGCAGAAATGGAGAATGTTAAGGACAGGACAAGACGTGAAGCAGAGAACTCAAAGAAGTTTGCAATTCAGGTTTCATGAGTTGGCCTTTCTAGAACGCTCTAAATTTTCCATTTCCTTCCTCAAACTCTGTCCTTCCTCCTGATACCGGTCCGTTTAACATGAACTTACATGTCTTTGAATTTGCTCTACAGAATTTTGCAAAGAGTTTGCTGGATGTTGCTGACAACTTGGGAAGAGCCTCGTCTGTGGTTAAAGACAGCTTTTCAAAAATTAAGGAATCTGAGGACTCGAGTGGCGCCGTACCTCTTCTAAAAACCCTTCTAGAAGGGGTCGATATGACTGAGAAACAACTCGTGCAGGTAAATTTATATGTTGCTTGCCATTTTCTAGCATGAGCTACTCAACATGTCGAGAGGAAATCTCAAGTTAAATCTATAGTCGTGATGCTTAGAGACACTTGTTCAACAGGTATTTAAGAAGTCCGGGGTAGAGAAATTCGACCCTACGAGCGAGCCATTCGATCCACATAGGCATAACGCGGTATTCCAAATCTCAGATGGGTCTAAGCCAGATGGCACTGTTGCTATTGTCCTAAAGGTATTAGCTTAGCTCAATTTGCAACTCTGCTTATTCTCATAAAAACAGGGTGGTAGACTGGTAGTTGGAGATATTTAGTTGATATGCTAAGGTAAAATTGTCCTTTAGACTGAGCTATAAACAAATTTTGTATTTTTTTTAGAAAACAAGTATTTTATTTCAACTTTTTTCAAGAACATGAGATCATCACTGATTATTTTAGAAGTTTGGGTTTGATATAGACGCTTTAGATGTGGAGATGATTTGATTTTCATGTTTTCATGACAGTCTGGATATATGCTATACGATCGAGTTCTTCGGCCAGCCGAAGTTGGTGTGACTCGAGCAGCGGAAAGTAATCCAAGTGATAACGTGTAAGTCTACATAGAAGCTAAGCTACCATGGAAGGCTGTCAAATGTTGATGAAGAAAATGGCTATGGTTGTGATTGGATTATTCAAAACTTTCCATTTCTATGACAGGAAATGTTGCATGAGGTACGAAGCAATGATTCTCTCTTTCCAGTAGAATGAGAATAAAAGAATAGCATTGAGTTACTTAATAACAGTTTAGAAGAAATTAATTTTCCAATTCCCATGTGTTTGATAATAATAAGGAAACATACCTATGGCAGATTATAGAGCAAGGTTAATTATTTTATCCTCGAGTGAGTCAATTTTAACCCTAAACTTTGTAATTTCATACCTTGACAATCTTGGAGTTAAACAAGTGATTCAATTAATACCTCGAGCACGACACCATCTAGTCCTATACAGAATGAAGCCTAACACGACACATTTTCAATAATAATGGGTACTAGAGGTGAACATTTATTTTCGAAAAACCAACCTAGCTAACCGAACTGACTTCTACCAGCCAATTTAGACCGCGGTTCA

mRNA sequence

GAGAACCGCTTCAAACAAAAATAGCTCAAAACAACAGCCACTGTAGATCCCTGTTTCTCCGAGCAGCCGCCTCCGTTTTCCCCAAAATTTTCCAAAAAAGTTGGAAAGGGCTTGGAATTTCTGCAGCTTTGAAACAATCGATTTCGTTTTCCCAACTCAGCGTCCGCCATTTCAATCTCTTCTTCCCAATGTCCGTTTCGAGGGTTCTGTTTCGAGCTTCCACCAGGAGCCTGCGCCGGAGCTCGCCGATTCTCTCCAAGCAGTTTCGCGGTCTCGTTCATGAGACTTCCGGCAAGTTTGCTCCAGTTCAGGTGTCTCTATTACATCATATAGTCTTCAATCCATTCCCCTTCCAAAAATTTGGAATATCTTCTACTACTTCCCATGAATCTTCTGAAAATAAAGAGGGGAGTGCCAAACAAAGTAAGAATTATTCTACAACTAAAGATCCTACAAAAGATGAGAATTCTGAAGTTTCTGACCAAGCAGGAGAATTGAATTCTACATCGAACTCCCATTCCACCAAATCGAAATCAAGACAATGGAGAAGACGCACTATACGAACTGCCTTTTCTGATTCGGATTCCGAGGGTTGTGAGCAACCTTCCATGGATGATCTGGTAAAATCACTGGCGCGGAAGGAAGAACTTTTGAAGTCTAAGCACAAAGAGATTAAGAAAATGCAAGACAAAGTCCTCCGTTCTTATGCAGAAATGGAGAATGTTAAGGACAGGACAAGACGTGAAGCAGAGAACTCAAAGAAGTTTGCAATTCAGAATTTTGCAAAGAGTTTGCTGGATGTTGCTGACAACTTGGGAAGAGCCTCGTCTGTGGTTAAAGACAGCTTTTCAAAAATTAAGGAATCTGAGGACTCGAGTGGCGCCGTACCTCTTCTAAAAACCCTTCTAGAAGGGGTCGATATGACTGAGAAACAACTCGTGCAGGTATTTAAGAAGTCCGGGGTAGAGAAATTCGACCCTACGAGCGAGCCATTCGATCCACATAGGCATAACGCGGTATTCCAAATCTCAGATGGGTCTAAGCCAGATGGCACTGTTGCTATTGTCCTAAAGTCTGGATATATGCTATACGATCGAGTTCTTCGGCCAGCCGAAGTTGGTGTGACTCGAGCAGCGGAAAGTAATCCAAGTGATAACGTGTAAGTCTACATAGAAGCTAAGCTACCATGGAAGGCTGTCAAATGTTGATGAAGAAAATGGCTATGGTTGTGATTGGATTATTCAAAACTTTCCATTTCTATGACAGGAAATGTTGCATGAGGTACGAAGCAATGATTCTCTCTTTCCAGTAGAATGAGAATAAAAGAATAGCATTGAGTTACTTAATAACAGTTTAGAAGAAATTAATTTTCCAATTCCCATGTGTTTGATAATAATAAGGAAACATACCTATGGCAGATTATAGAGCAAGGTTAATTATTTTATCCTCGAGTGAGTCAATTTTAACCCTAAACTTTGTAATTTCATACCTTGACAATCTTGGAGTTAAACAAGTGATTCAATTAATACCTCGAGCACGACACCATCTAGTCCTATACAGAATGAAGCCTAACACGACACATTTTCAATAATAATGGGTACTAGAGGTGAACATTTATTTTCGAAAAACCAACCTAGCTAACCGAACTGACTTCTACCAGCCAATTTAGACCGCGGTTCA

Coding sequence (CDS)

GAACCGCTTCAAACAAAAATAGCTCAAAACAACAGCCACTGTAGATCCCTGTTTCTCCGAGCAGCCGCCTCCGTTTTCCCCAAAATTTTCCAAAAAAGTTGGAAAGGGCTTGGAATTTCTGCAGCTTTGAAACAATCGATTTCGTTTTCCCAACTCAGCGTCCGCCATTTCAATCTCTTCTTCCCAATGTCCGTTTCGAGGGTTCTGTTTCGAGCTTCCACCAGGAGCCTGCGCCGGAGCTCGCCGATTCTCTCCAAGCAGTTTCGCGGTCTCGTTCATGAGACTTCCGGCAAGTTTGCTCCAGTTCAGGTGTCTCTATTACATCATATAGTCTTCAATCCATTCCCCTTCCAAAAATTTGGAATATCTTCTACTACTTCCCATGAATCTTCTGAAAATAAAGAGGGGAGTGCCAAACAAAGTAAGAATTATTCTACAACTAAAGATCCTACAAAAGATGAGAATTCTGAAGTTTCTGACCAAGCAGGAGAATTGAATTCTACATCGAACTCCCATTCCACCAAATCGAAATCAAGACAATGGAGAAGACGCACTATACGAACTGCCTTTTCTGATTCGGATTCCGAGGGTTGTGAGCAACCTTCCATGGATGATCTGGTAAAATCACTGGCGCGGAAGGAAGAACTTTTGAAGTCTAAGCACAAAGAGATTAAGAAAATGCAAGACAAAGTCCTCCGTTCTTATGCAGAAATGGAGAATGTTAAGGACAGGACAAGACGTGAAGCAGAGAACTCAAAGAAGTTTGCAATTCAGAATTTTGCAAAGAGTTTGCTGGATGTTGCTGACAACTTGGGAAGAGCCTCGTCTGTGGTTAAAGACAGCTTTTCAAAAATTAAGGAATCTGAGGACTCGAGTGGCGCCGTACCTCTTCTAAAAACCCTTCTAGAAGGGGTCGATATGACTGAGAAACAACTCGTGCAGGTATTTAAGAAGTCCGGGGTAGAGAAATTCGACCCTACGAGCGAGCCATTCGATCCACATAGGCATAACGCGGTATTCCAAATCTCAGATGGGTCTAAGCCAGATGGCACTGTTGCTATTGTCCTAAAGTCTGGATATATGCTATACGATCGAGTTCTTCGGCCAGCCGAAGTTGGTGTGACTCGAGCAGCGGAAAGTAATCCAAGTGATAACGTGTAA

Protein sequence

EPLQTKIAQNNSHCRSLFLRAAASVFPKIFQKSWKGLGISAALKQSISFSQLSVRHFNLFFPMSVSRVLFRASTRSLRRSSPILSKQFRGLVHETSGKFAPVQVSLLHHIVFNPFPFQKFGISSTTSHESSENKEGSAKQSKNYSTTKDPTKDENSEVSDQAGELNSTSNSHSTKSKSRQWRRRTIRTAFSDSDSEGCEQPSMDDLVKSLARKEELLKSKHKEIKKMQDKVLRSYAEMENVKDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIKESEDSSGAVPLLKTLLEGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKPDGTVAIVLKSGYMLYDRVLRPAEVGVTRAAESNPSDNV

Homology

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BLAST of MC11g1004.1 vs. ExPASy Swiss-Prot
Match: Q8LB47 (GrpE protein homolog 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=Mge2 PE=1 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 2.4e-79
Identity = 172/310 (55.48%), Postives = 224/310 (72.26%), Query Frame = 0


Query: 72  ASTRSLRRSSPILSKQFRGLVHETSGKFAPVQVSLLHHIVFNPFPFQKFGISSTTSHESS 131
           A T   R  +P+ S +F  L H+ S K  P Q+S++     + F  Q+F  SS+TS ES 
Sbjct: 22  AVTLPARNQTPVFSSRFHSLAHDFSHKLVPAQMSMM-----DSFALQRFNFSSSTSPESD 81

Query: 132 ENKEGSAKQSKNYSTTKDPTKDENSEVSDQAGELNSTSNSHSTKSKSRQWRRRTIRTAFS 191
           E K        + ++ + PT + N     Q G  + + +S +  +K ++   +   ++ S
Sbjct: 82  EKK---THTEASKTSEEKPTAEAN-----QPGLDSESKDSVTDSAKRKRKGAKGAASSSS 141

Query: 192 DSDSEG-CEQPSMDDLVKSLARKEELLKSKHKEIKKMQDKVLRSYAEMENVKDRTRREAE 251
           +SDSE   ++ S DDLVK +A KEELL  K +EIK+++DKVLR+YAEMENV DRTRR+AE
Sbjct: 142 ESDSESDDDELSADDLVKLVAEKEELLSEKEEEIKQLKDKVLRTYAEMENVMDRTRRDAE 201

Query: 252 NSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIKESEDSSGAVPLLKTLLEGVDMTEK 311
           N+KK+A+QNFAKSLLDVADNLGRASSVVK+SFSK+  SEDS+GA PLLKTLLEGV+MTEK
Sbjct: 202 NTKKYAVQNFAKSLLDVADNLGRASSVVKESFSKLDTSEDSAGAAPLLKTLLEGVEMTEK 261

Query: 312 QLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKPDGTVAIVLKSGYMLYDRVLRPA 371
           QL +VFKK G+EK+DP +EPFDP+RHNAVFQ+ D SKP+GTVA VLKSGY LYDRV+RPA
Sbjct: 262 QLAEVFKKFGMEKYDPINEPFDPNRHNAVFQVPDASKPEGTVAHVLKSGYTLYDRVIRPA 318

Query: 372 EVGVTRAAES 381
           EVGVT+  E+
Sbjct: 322 EVGVTQGGEN 318

[Close]

BLAST of MC11g1004.1 vs. ExPASy Swiss-Prot
Match: Q9FLP3 (GrpE protein homolog 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=Mge1 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 5.8e-65
Identity = 159/330 (48.18%), Postives = 201/330 (60.91%), Query Frame = 0


Query: 63  MSVSRVLFRASTRSLRRSS------------PILSKQFRGLVHETSGKFAPVQVSLLHHI 122
           M VSRVL R S  +  RSS            PI++ +F  LVH T  K   V VSL +H 
Sbjct: 1   MLVSRVLSRVSRSAGLRSSFSSVVTPKRNQIPIVASRFHSLVHGTPNKLVAVPVSLRNHG 60

Query: 123 VFNPFPFQKFGISSTTSHESSENKEGSAKQSKNYSTTKDPTKDENSEVSDQAGELNSTSN 182
             +    Q+FG  S++S E   N+          S T+ P   E SE             
Sbjct: 61  TLDLNVLQRFGFFSSSSAEPKGNE----------SNTEVPKTGETSE------------- 120

Query: 183 SHSTKSKSRQWRRRTIRTAFSDSDSEGCEQPSMDDLVKSLARKEELLKSKHKEIKKMQDK 242
                          +    +       +  S DDLVK ++ KE+LLK + K+I +M+DK
Sbjct: 121 --------------NVEVGKATDAEIDFDDLSRDDLVKLVSEKEDLLKVQQKDIMEMKDK 180

Query: 243 VLRSYAEMENVKDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIKESED 302
            LR+YAE +N+ DRT R AE++KKFA+QNFA SLLDVADNL RASSVVK+SFSKI  S+D
Sbjct: 181 FLRTYAEQQNLMDRTNRNAESAKKFAVQNFATSLLDVADNLERASSVVKESFSKIDTSKD 240

Query: 303 SSGAVPLLKTLLEGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKPDG 362
            +GA PLLK LLEGV+MTEKQL +VF+K+G+ K DP +EPF+P+RHNAVFQ+ D SKP G
Sbjct: 241 LAGATPLLKNLLEGVEMTEKQLAEVFRKAGLVKEDPLNEPFNPNRHNAVFQVPDASKPKG 293

Query: 363 TVAIVLKSGYMLYDRVLRPAEVGVTRAAES 381
           T+A VLKSGY LYDRV+RPAEVGVT A E+
Sbjct: 301 TIAHVLKSGYSLYDRVIRPAEVGVTCAVEN 293

[Close]

BLAST of MC11g1004.1 vs. ExPASy Swiss-Prot
Match: A4YJR1 (Protein GrpE OS=Bradyrhizobium sp. (strain ORS 278) OX=114615 GN=grpE PE=3 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 6.6e-29
Identity = 72/170 (42.35%), Postives = 110/170 (64.71%), Query Frame = 0


Query: 222 KEIKKMQDKVLRSYAEMENVKDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDS 281
           KE  + +DK+LR+ AEMEN++ RT RE  +++ + I  FA+ +LD+ADNL RA   V   
Sbjct: 42  KEAAEARDKMLRTLAEMENLRKRTAREVADARMYGITGFARDVLDIADNLQRALDAV--- 101

Query: 282 FSKIKESEDSSGAVPLLKTLLEGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQ 341
                 +E  + A   LK+L+EGV++TE+ L+   +K+GV+KFDPT + FDP+   A+++
Sbjct: 102 -----PAETRANADAGLKSLIEGVELTERSLLNTLEKNGVKKFDPTGQKFDPNFQQAMYE 161

Query: 342 ISDGSKPDGTVAIVLKSGYMLYDRVLRPAEVGVTRA------AESNPSDN 386
           + D S P GTV  V+++G+M+ +RVLRPA VGV++       A SN   N
Sbjct: 162 VPDPSVPSGTVVQVVQAGFMIGERVLRPALVGVSKGGAKAAPAASNDQSN 203

[Close]

BLAST of MC11g1004.1 vs. ExPASy Swiss-Prot
Match: B6JCI1 (Protein GrpE OS=Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5) OX=504832 GN=grpE PE=3 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 6.6e-29
Identity = 69/156 (44.23%), Postives = 105/156 (67.31%), Query Frame = 0


Query: 222 KEIKKMQDKVLRSYAEMENVKDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDS 281
           KE  + +D++LR+ AEMEN++ RT+RE  +++ + I  FA+ +L++ADNL RA   V   
Sbjct: 47  KEAAEAKDRMLRTLAEMENLRKRTQREVADARAYGIAGFARDVLEIADNLQRALDAV--- 106

Query: 282 FSKIKESEDSSGAVPLLKTLLEGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQ 341
                 +E  + A P L  L+EGV++TE+ L +  +K+GV+K D   E FDP+ H A+F+
Sbjct: 107 -----PAEARAAADPGLTALIEGVELTERSLHRALEKNGVKKLDAAGEKFDPNIHQAMFE 166

Query: 342 ISDGSKPDGTVAIVLKSGYMLYDRVLRPAEVGVTRA 378
           + D S P GTV  V+++GYM+ DRVLRPA VGV++A
Sbjct: 167 VPDNSVPPGTVVQVIQTGYMIGDRVLRPALVGVSKA 194

[Close]

BLAST of MC11g1004.1 vs. ExPASy Swiss-Prot
Match: B3Q970 (Protein GrpE OS=Rhodopseudomonas palustris (strain TIE-1) OX=395960 GN=grpE PE=3 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 1.1e-28
Identity = 69/164 (42.07%), Postives = 110/164 (67.07%), Query Frame = 0


Query: 222 KEIKKMQDKVLRSYAEMENVKDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDS 281
           +E    +DK+LR+ AEMEN++ RT++E  +++ + + +FA+ +LD+ADNL RA   V   
Sbjct: 43  REAADARDKMLRTLAEMENLRKRTQKEVADARTYGVTSFARDVLDIADNLQRALDAV--- 102

Query: 282 FSKIKESEDSSGAVPLLKTLLEGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQ 341
                 +E  + A P LK L+EGV++TE+ L+   +K+GV+KFDP  + FDP+   A+++
Sbjct: 103 -----PAEARANAEPGLKALIEGVELTERSLLNALEKNGVKKFDPKGQKFDPNFQQAMYE 162

Query: 342 ISDGSKPDGTVAIVLKSGYMLYDRVLRPAEVGVTR-AAESNPSD 385
           + D S P GTV  V+++G+ + DRVLRPA VGV +  A++ PSD
Sbjct: 163 VPDPSVPAGTVVQVVQAGFTIGDRVLRPALVGVAKGGAKAAPSD 198

[Close]

BLAST of MC11g1004.1 vs. NCBI nr
Match: XP_022139414.1 (uncharacterized protein LOC111010351 [Momordica charantia])

HSP 1 Score: 605 bits (1559), Expect = 2.43e-216
Identity = 324/324 (100.00%), Postives = 324/324 (100.00%), Query Frame = 0


Query: 63  MSVSRVLFRASTRSLRRSSPILSKQFRGLVHETSGKFAPVQVSLLHHIVFNPFPFQKFGI 122
           MSVSRVLFRASTRSLRRSSPILSKQFRGLVHETSGKFAPVQVSLLHHIVFNPFPFQKFGI
Sbjct: 1   MSVSRVLFRASTRSLRRSSPILSKQFRGLVHETSGKFAPVQVSLLHHIVFNPFPFQKFGI 60

Query: 123 SSTTSHESSENKEGSAKQSKNYSTTKDPTKDENSEVSDQAGELNSTSNSHSTKSKSRQWR 182
           SSTTSHESSENKEGSAKQSKNYSTTKDPTKDENSEVSDQAGELNSTSNSHSTKSKSRQWR
Sbjct: 61  SSTTSHESSENKEGSAKQSKNYSTTKDPTKDENSEVSDQAGELNSTSNSHSTKSKSRQWR 120

Query: 183 RRTIRTAFSDSDSEGCEQPSMDDLVKSLARKEELLKSKHKEIKKMQDKVLRSYAEMENVK 242
           RRTIRTAFSDSDSEGCEQPSMDDLVKSLARKEELLKSKHKEIKKMQDKVLRSYAEMENVK
Sbjct: 121 RRTIRTAFSDSDSEGCEQPSMDDLVKSLARKEELLKSKHKEIKKMQDKVLRSYAEMENVK 180

Query: 243 DRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIKESEDSSGAVPLLKTLL 302
           DRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIKESEDSSGAVPLLKTLL
Sbjct: 181 DRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIKESEDSSGAVPLLKTLL 240

Query: 303 EGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKPDGTVAIVLKSGYML 362
           EGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKPDGTVAIVLKSGYML
Sbjct: 241 EGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKPDGTVAIVLKSGYML 300

Query: 363 YDRVLRPAEVGVTRAAESNPSDNV 386
           YDRVLRPAEVGVTRAAESNPSDNV
Sbjct: 301 YDRVLRPAEVGVTRAAESNPSDNV 324

[Close]

BLAST of MC11g1004.1 vs. NCBI nr
Match: XP_038898199.1 (grpE protein homolog 2, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 469 bits (1207), Expect = 3.92e-162
Identity = 270/342 (78.95%), Postives = 294/342 (85.96%), Query Frame = 0


Query: 52  LSVRHFNLFFPMSVSRVLFRASTRSLRRSSPILS---------KQFRGLVHETSGKFAPV 111
           L+VR +N FF MSVSR+LFRAS RSL RSSPIL          +QF GLVHE SGK APV
Sbjct: 29  LTVRLYN-FFSMSVSRILFRAS-RSLHRSSPILYGYQTFPILYRQFHGLVHEISGKSAPV 88

Query: 112 QVSLLHHIVFNPFPFQKFGISSTTSHESSENKEGSAKQSKNYSTTKDPTKDENSEVSDQA 171
           QVSLL+HIV+NPFPFQ+FG+SST  HESSEN EGSAK S   ST   PTK  NSEVSD+A
Sbjct: 89  QVSLLNHIVYNPFPFQRFGVSSTAIHESSEN-EGSAKHS--GSTNNGPTKHGNSEVSDRA 148

Query: 172 GELNSTSNSHSTKSKSRQWRRRTIRTAFSDSDSEGCEQPSMDDLVKSLARKEELLKSKHK 231
            E   T +SHSTKSKSR+  RR++RTAFSDSDSE  EQPSMDDLVKSLARKEELLKSKHK
Sbjct: 149 EESTFTLDSHSTKSKSRRRSRRSVRTAFSDSDSESDEQPSMDDLVKSLARKEELLKSKHK 208

Query: 232 EIKKMQDKVLRSYAEMENVKDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSF 291
           E++KMQDKVLRSYAEMENV DRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSF
Sbjct: 209 ELEKMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSF 268

Query: 292 SKIKESEDSSGAVPLLKTLLEGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQI 351
           SKI ES+DSSGAVPLLK+LLEGVDMTEKQL++ F K GVEKFDPTSEPFDPH+H AVFQI
Sbjct: 269 SKINESQDSSGAVPLLKSLLEGVDMTEKQLMEAFTKFGVEKFDPTSEPFDPHKHFAVFQI 328

Query: 352 SDGSKPDGTVAIVLKSGYMLYDRVLRPAEVGVTRAAESNPSD 384
            DGSKPDGTVAIVLKSGYML+DRVLRPAEVGVT+AAE N ++
Sbjct: 329 PDGSKPDGTVAIVLKSGYMLHDRVLRPAEVGVTQAAEDNSTN 365

[Close]

BLAST of MC11g1004.1 vs. NCBI nr
Match: XP_022936267.1 (grpE protein homolog 2, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 452 bits (1162), Expect = 6.29e-156
Identity = 260/336 (77.38%), Postives = 282/336 (83.93%), Query Frame = 0


Query: 63  MSVSRVLFRASTRSLRRSSPILSK--------------QFRGLVHETSGKFAPVQVSLLH 122
           MS  R+L RAS RSL +SSPIL +              QF GLV E SGK APV VSLL+
Sbjct: 1   MSSFRILVRAS-RSLHQSSPILIRNQSQNCRTFPILYRQFHGLVDEISGKSAPVHVSLLN 60

Query: 123 HIVFNPFPFQKFGISSTTSHESSENKEGSAKQSKNYSTTKDPTKDENSEVSDQAGELNST 182
           HIV NPFPFQ+FG+SST SHESSEN+EGSAKQ            D NSEVS+QA EL+ST
Sbjct: 61  HIVSNPFPFQRFGVSSTASHESSENEEGSAKQD-----------DVNSEVSNQAEELSST 120

Query: 183 SNSHSTKSKSRQWRRRTIRTAFSDSDSEGCEQPSMDDLVKSLARKEELLKSKHKEIKKMQ 242
           ++SHSTKSKSR+ RRRTIRTAFSDSDSE  EQPSMDDLVKSLARKEELLKSKHKEI+KMQ
Sbjct: 121 TDSHSTKSKSRRQRRRTIRTAFSDSDSECDEQPSMDDLVKSLARKEELLKSKHKEIEKMQ 180

Query: 243 DKVLRSYAEMENVKDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIKES 302
           +KVL SYAEMENV DRT+REAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKI ES
Sbjct: 181 EKVLCSYAEMENVMDRTKREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIDES 240

Query: 303 EDSSGAVPLLKTLLEGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKP 362
           ED SGAV LLKTLLEGVDMTEKQL++VFKKSGVEKFDPTSEPFDPH+H AVFQI DGSKP
Sbjct: 241 EDPSGAVALLKTLLEGVDMTEKQLMEVFKKSGVEKFDPTSEPFDPHKHYAVFQIPDGSKP 300

Query: 363 DGTVAIVLKSGYMLYDRVLRPAEVGVTRAAESNPSD 384
           DGTVA+VLKSGYML+DRVLRPAEVGVT+A E N +D
Sbjct: 301 DGTVAVVLKSGYMLHDRVLRPAEVGVTQAVEDNSTD 324

[Close]

BLAST of MC11g1004.1 vs. NCBI nr
Match: XP_023536002.1 (grpE protein homolog 2, mitochondrial-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 448 bits (1153), Expect = 1.32e-154
Identity = 258/333 (77.48%), Postives = 279/333 (83.78%), Query Frame = 0


Query: 63  MSVSRVLFRASTRSLRRSSPILS--------------KQFRGLVHETSGKFAPVQVSLLH 122
           MS S +LFRAS RSL RSSPIL               +QF GLV E SGK APV VSLL+
Sbjct: 1   MSSSSILFRAS-RSLHRSSPILIGNQSQNCRTFPILYRQFHGLVDEISGKSAPVHVSLLN 60

Query: 123 HIVFNPFPFQKFGISSTTSHESSENKEGSAKQSKNYSTTKDPTKDENSEVSDQAGELNST 182
           HIVFNPFPFQ+FG+SST SHESSEN+E SAKQ              NSEVS+QA EL+ST
Sbjct: 61  HIVFNPFPFQRFGLSSTASHESSENEERSAKQGT-----------VNSEVSNQAEELSST 120

Query: 183 SNSHSTKSKSRQWRRRTIRTAFSDSDSEGCEQPSMDDLVKSLARKEELLKSKHKEIKKMQ 242
           ++SHSTKSKSR+ RRRTIRTAFS+SDSE  EQPSMDDLVKSL RKEELLKSKHKEI+KMQ
Sbjct: 121 TDSHSTKSKSRRQRRRTIRTAFSNSDSESDEQPSMDDLVKSLTRKEELLKSKHKEIEKMQ 180

Query: 243 DKVLRSYAEMENVKDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIKES 302
           +KVL SYAEMENV DRT+REAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKI ES
Sbjct: 181 EKVLCSYAEMENVMDRTKREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIDES 240

Query: 303 EDSSGAVPLLKTLLEGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKP 362
           ED SGAV LLKTLLEGVDMTEKQL++VFKKSGVEKFDPTSEPFDPH+H AVFQI DGSKP
Sbjct: 241 EDPSGAVALLKTLLEGVDMTEKQLMEVFKKSGVEKFDPTSEPFDPHKHYAVFQIPDGSKP 300

Query: 363 DGTVAIVLKSGYMLYDRVLRPAEVGVTRAAESN 381
           DGTVA+VLKSGYML+DRVLRPAEVGVT+A E N
Sbjct: 301 DGTVAVVLKSGYMLHDRVLRPAEVGVTQAVEDN 321

[Close]

BLAST of MC11g1004.1 vs. NCBI nr
Match: KAG7024936.1 (grpE, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 444 bits (1143), Expect = 5.21e-153
Identity = 258/338 (76.33%), Postives = 281/338 (83.14%), Query Frame = 0


Query: 63  MSVSRVLFRASTRSLRRSSPILS--------------KQFRGLVHETSGKF--APVQVSL 122
           MS  R+LFRAS RSL +SSPIL               +QF GLV E SGK   APV VSL
Sbjct: 1   MSSFRILFRAS-RSLHQSSPILIGNQSQNCRTFPILYRQFHGLVDEISGKVESAPVHVSL 60

Query: 123 LHHIVFNPFPFQKFGISSTTSHESSENKEGSAKQSKNYSTTKDPTKDENSEVSDQAGELN 182
           L+HIV NPFPFQ+ G+SST SHESSEN+EGSAKQ            + NSEVS+QA EL+
Sbjct: 61  LNHIVSNPFPFQRLGVSSTASHESSENEEGSAKQD-----------NVNSEVSNQAEELS 120

Query: 183 STSNSHSTKSKSRQWRRRTIRTAFSDSDSEGCEQPSMDDLVKSLARKEELLKSKHKEIKK 242
           ST++SHSTKSKSR+ RRRTIRTAFS+SDSE  EQPSMDDLVKSLARKEELLKSKHKEI K
Sbjct: 121 STTDSHSTKSKSRRQRRRTIRTAFSESDSESDEQPSMDDLVKSLARKEELLKSKHKEIDK 180

Query: 243 MQDKVLRSYAEMENVKDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIK 302
           MQ+KVL SYAEMENV DRT+REAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKI 
Sbjct: 181 MQEKVLCSYAEMENVMDRTKREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKID 240

Query: 303 ESEDSSGAVPLLKTLLEGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGS 362
           ESED SGAV LLKTLLEGVDMTEKQL++VFKKSGVEKFDPTSEPFDPH+H AVFQI DGS
Sbjct: 241 ESEDPSGAVALLKTLLEGVDMTEKQLMEVFKKSGVEKFDPTSEPFDPHKHYAVFQIPDGS 300

Query: 363 KPDGTVAIVLKSGYMLYDRVLRPAEVGVTRAAESNPSD 384
           KPDGTVA+VLKSGYML+DRVLRPAEVGVT+A E N +D
Sbjct: 301 KPDGTVAVVLKSGYMLHDRVLRPAEVGVTQAVEDNSTD 326

[Close]

BLAST of MC11g1004.1 vs. ExPASy TrEMBL
Match: A0A6J1CCY7 (GrpE protein homolog OS=Momordica charantia OX=3673 GN=LOC111010351 PE=3 SV=1)

HSP 1 Score: 605 bits (1559), Expect = 1.18e-216
Identity = 324/324 (100.00%), Postives = 324/324 (100.00%), Query Frame = 0


Query: 63  MSVSRVLFRASTRSLRRSSPILSKQFRGLVHETSGKFAPVQVSLLHHIVFNPFPFQKFGI 122
           MSVSRVLFRASTRSLRRSSPILSKQFRGLVHETSGKFAPVQVSLLHHIVFNPFPFQKFGI
Sbjct: 1   MSVSRVLFRASTRSLRRSSPILSKQFRGLVHETSGKFAPVQVSLLHHIVFNPFPFQKFGI 60

Query: 123 SSTTSHESSENKEGSAKQSKNYSTTKDPTKDENSEVSDQAGELNSTSNSHSTKSKSRQWR 182
           SSTTSHESSENKEGSAKQSKNYSTTKDPTKDENSEVSDQAGELNSTSNSHSTKSKSRQWR
Sbjct: 61  SSTTSHESSENKEGSAKQSKNYSTTKDPTKDENSEVSDQAGELNSTSNSHSTKSKSRQWR 120

Query: 183 RRTIRTAFSDSDSEGCEQPSMDDLVKSLARKEELLKSKHKEIKKMQDKVLRSYAEMENVK 242
           RRTIRTAFSDSDSEGCEQPSMDDLVKSLARKEELLKSKHKEIKKMQDKVLRSYAEMENVK
Sbjct: 121 RRTIRTAFSDSDSEGCEQPSMDDLVKSLARKEELLKSKHKEIKKMQDKVLRSYAEMENVK 180

Query: 243 DRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIKESEDSSGAVPLLKTLL 302
           DRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIKESEDSSGAVPLLKTLL
Sbjct: 181 DRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIKESEDSSGAVPLLKTLL 240

Query: 303 EGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKPDGTVAIVLKSGYML 362
           EGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKPDGTVAIVLKSGYML
Sbjct: 241 EGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKPDGTVAIVLKSGYML 300

Query: 363 YDRVLRPAEVGVTRAAESNPSDNV 386
           YDRVLRPAEVGVTRAAESNPSDNV
Sbjct: 301 YDRVLRPAEVGVTRAAESNPSDNV 324

[Close]

BLAST of MC11g1004.1 vs. ExPASy TrEMBL
Match: A0A6J1F7Z2 (GrpE protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111442938 PE=3 SV=1)

HSP 1 Score: 452 bits (1162), Expect = 3.04e-156
Identity = 260/336 (77.38%), Postives = 282/336 (83.93%), Query Frame = 0


Query: 63  MSVSRVLFRASTRSLRRSSPILSK--------------QFRGLVHETSGKFAPVQVSLLH 122
           MS  R+L RAS RSL +SSPIL +              QF GLV E SGK APV VSLL+
Sbjct: 1   MSSFRILVRAS-RSLHQSSPILIRNQSQNCRTFPILYRQFHGLVDEISGKSAPVHVSLLN 60

Query: 123 HIVFNPFPFQKFGISSTTSHESSENKEGSAKQSKNYSTTKDPTKDENSEVSDQAGELNST 182
           HIV NPFPFQ+FG+SST SHESSEN+EGSAKQ            D NSEVS+QA EL+ST
Sbjct: 61  HIVSNPFPFQRFGVSSTASHESSENEEGSAKQD-----------DVNSEVSNQAEELSST 120

Query: 183 SNSHSTKSKSRQWRRRTIRTAFSDSDSEGCEQPSMDDLVKSLARKEELLKSKHKEIKKMQ 242
           ++SHSTKSKSR+ RRRTIRTAFSDSDSE  EQPSMDDLVKSLARKEELLKSKHKEI+KMQ
Sbjct: 121 TDSHSTKSKSRRQRRRTIRTAFSDSDSECDEQPSMDDLVKSLARKEELLKSKHKEIEKMQ 180

Query: 243 DKVLRSYAEMENVKDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIKES 302
           +KVL SYAEMENV DRT+REAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKI ES
Sbjct: 181 EKVLCSYAEMENVMDRTKREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIDES 240

Query: 303 EDSSGAVPLLKTLLEGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKP 362
           ED SGAV LLKTLLEGVDMTEKQL++VFKKSGVEKFDPTSEPFDPH+H AVFQI DGSKP
Sbjct: 241 EDPSGAVALLKTLLEGVDMTEKQLMEVFKKSGVEKFDPTSEPFDPHKHYAVFQIPDGSKP 300

Query: 363 DGTVAIVLKSGYMLYDRVLRPAEVGVTRAAESNPSD 384
           DGTVA+VLKSGYML+DRVLRPAEVGVT+A E N +D
Sbjct: 301 DGTVAVVLKSGYMLHDRVLRPAEVGVTQAVEDNSTD 324

[Close]

BLAST of MC11g1004.1 vs. ExPASy TrEMBL
Match: M5WTU3 (GrpE protein homolog OS=Prunus persica OX=3760 GN=PRUPE_4G223200 PE=3 SV=1)

HSP 1 Score: 357 bits (916), Expect = 1.09e-118
Identity = 214/336 (63.69%), Postives = 255/336 (75.89%), Query Frame = 0


Query: 63  MSVSRVLFRASTRSLRRSS-----------PILSKQFRGLVHETSGKFAPVQVSLLHHIV 122
           M VSRVL RAS R+L RS+           PIL  Q + L+H+ S KF+P QVSLLHH  
Sbjct: 1   MFVSRVLSRAS-RTLPRSTMLIAAPQNHHLPILCNQSQALIHDFSIKFSPRQVSLLHHST 60

Query: 123 FNPFPFQKFGISSTTSHESSENKEGSAKQSKNYSTTKDPTKDE-NSEVSDQAGELNSTSN 182
            +    Q FG SS+ S + SE + GSA ++   ST  +P K+  +++VSDQ  E   TS+
Sbjct: 61  PSSSISQIFGFSSSASPQPSEREHGSAAENGGASTNGEPAKESGDAKVSDQTKESGYTSD 120

Query: 183 SHSTKSKSRQWRRR-TIRTAFSDSDSEGCEQPSMDDLVKSLARKEELLKSKHKEIKKMQD 242
           S S KS S + RR  T RTAFSDSDSEG      DDLVK +A KEELLK KH+EIK+MQD
Sbjct: 121 SQSKKSPSVKRRRGGTKRTAFSDSDSEGEGDLGFDDLVKLVAEKEELLKQKHEEIKRMQD 180

Query: 243 KVLRSYAEMENVKDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIKESE 302
           KVLRSYAEMENV DRTRREAENSKKFA+QNFAKSLLDVADNLGRASSVVKDSFSK+ ES+
Sbjct: 181 KVLRSYAEMENVMDRTRREAENSKKFAVQNFAKSLLDVADNLGRASSVVKDSFSKLDESK 240

Query: 303 DSSGAVPLLKTLLEGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKPD 362
           +S GA PLLKTLLEGV+MTEKQL++VF+K GVEKFDPT+E FDP++HNAVF + D SKP 
Sbjct: 241 ESGGAAPLLKTLLEGVEMTEKQLIEVFRKYGVEKFDPTNEAFDPNKHNAVFNLEDASKPP 300

Query: 363 GTVAIVLKSGYMLYDRVLRPAEVGVTRAAESNPSDN 385
           GT+A+VLK GYMLYDRV+RPAEVGVT A E+N +++
Sbjct: 301 GTIAVVLKPGYMLYDRVVRPAEVGVTTAPENNATED 335

[Close]

BLAST of MC11g1004.1 vs. ExPASy TrEMBL
Match: A0A5E4FCQ4 (GrpE protein homolog OS=Prunus dulcis OX=3755 GN=ALMOND_2B019776 PE=3 SV=1)

HSP 1 Score: 357 bits (916), Expect = 1.09e-118
Identity = 214/336 (63.69%), Postives = 255/336 (75.89%), Query Frame = 0


Query: 63  MSVSRVLFRASTRSLRRSS-----------PILSKQFRGLVHETSGKFAPVQVSLLHHIV 122
           M VSRVL RAS R+L RS+           PIL  Q + L+H+ S KF+P QVSLLHH  
Sbjct: 1   MFVSRVLSRAS-RTLPRSTMLIAAPQNHHLPILCNQSQALIHDFSIKFSPRQVSLLHHST 60

Query: 123 FNPFPFQKFGISSTTSHESSENKEGSAKQSKNYSTTKDPTKDE-NSEVSDQAGELNSTSN 182
            +    Q FG SS+ S + SE + GSA ++   ST  +P K+  +++VSDQ  E   TS+
Sbjct: 61  PSSSISQIFGFSSSASPQPSEREHGSAAENGGASTNGEPAKESGDAKVSDQTKESGYTSD 120

Query: 183 SHSTKSKSRQWRRR-TIRTAFSDSDSEGCEQPSMDDLVKSLARKEELLKSKHKEIKKMQD 242
           S S KS S + RR  T RTAFSDSDSEG      DDLVK +A KEELLK KH+EIK+MQD
Sbjct: 121 SQSKKSPSVKRRRGGTKRTAFSDSDSEGEGDLGFDDLVKLVAEKEELLKQKHEEIKRMQD 180

Query: 243 KVLRSYAEMENVKDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIKESE 302
           KVLRSYAEMENV DRTRREAENSKKFA+QNFAKSLLDVADNLGRASSVVKDSFSK+ ES+
Sbjct: 181 KVLRSYAEMENVMDRTRREAENSKKFAVQNFAKSLLDVADNLGRASSVVKDSFSKLDESK 240

Query: 303 DSSGAVPLLKTLLEGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKPD 362
           +S GA PLLKTLLEGV+MTEKQL++VF+K GVEKFDPT+E FDP++HNAVF + D SKP 
Sbjct: 241 ESGGAAPLLKTLLEGVEMTEKQLIEVFRKYGVEKFDPTNEAFDPNKHNAVFNLEDASKPP 300

Query: 363 GTVAIVLKSGYMLYDRVLRPAEVGVTRAAESNPSDN 385
           GT+A+VLK GYMLYDRV+RPAEVGVT A E+N +++
Sbjct: 301 GTIAVVLKPGYMLYDRVVRPAEVGVTTAPENNATED 335

[Close]

BLAST of MC11g1004.1 vs. ExPASy TrEMBL
Match: A0A6J5X7K4 (GrpE protein homolog OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS28266 PE=3 SV=1)

HSP 1 Score: 355 bits (912), Expect = 4.40e-118
Identity = 214/336 (63.69%), Postives = 254/336 (75.60%), Query Frame = 0


Query: 63  MSVSRVLFRASTRSLRRSS-----------PILSKQFRGLVHETSGKFAPVQVSLLHHIV 122
           M VSRVL RAS R+L RS+           PIL  Q + L+H+ S KF+P QVSLLHH  
Sbjct: 1   MFVSRVLTRAS-RTLPRSTMLFAAPQNHHLPILCNQSQALIHDFSIKFSPRQVSLLHHST 60

Query: 123 FNPFPFQKFGISSTTSHESSENKEGSAKQSKNYSTTKDPTKDE-NSEVSDQAGELNSTSN 182
            +    Q FG SS+ S + SE + GSA ++   ST  +P K   +++VSDQ  E   TS+
Sbjct: 61  PSSSISQIFGFSSSASPQPSEREHGSAAENGGASTNGEPAKASGDAKVSDQTKESGYTSD 120

Query: 183 SHSTKSKSRQWRRR-TIRTAFSDSDSEGCEQPSMDDLVKSLARKEELLKSKHKEIKKMQD 242
           S S KS S + RR  T RTAFSDSDSEG      DDLVK +A KEELLK KH+EIK+MQD
Sbjct: 121 SQSKKSPSVKRRRGGTKRTAFSDSDSEGEGDLGFDDLVKLVAEKEELLKQKHEEIKRMQD 180

Query: 243 KVLRSYAEMENVKDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIKESE 302
           KVLRSYAEMENV DRTRREAENSKKFA+QNFAKSLLDVADNLGRASSVVKDSFSK+ ES+
Sbjct: 181 KVLRSYAEMENVMDRTRREAENSKKFAVQNFAKSLLDVADNLGRASSVVKDSFSKLDESK 240

Query: 303 DSSGAVPLLKTLLEGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKPD 362
           +S GA PLLKTLLEGV+MTEKQL++VF+K GVEKFDPT+E FDP++HNAVF + D SKP 
Sbjct: 241 ESGGAAPLLKTLLEGVEMTEKQLIEVFRKYGVEKFDPTNEAFDPNKHNAVFNLEDASKPP 300

Query: 363 GTVAIVLKSGYMLYDRVLRPAEVGVTRAAESNPSDN 385
           GT+A+VLK GYMLYDRV+RPAEVGVT A E+N +++
Sbjct: 301 GTIAVVLKPGYMLYDRVVRPAEVGVTTAPENNATED 335

[Close]

BLAST of MC11g1004.1 vs. TAIR 10
Match: AT4G26780.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 297.4 bits (760), Expect = 1.7e-80
Identity = 172/310 (55.48%), Postives = 224/310 (72.26%), Query Frame = 0


Query: 72  ASTRSLRRSSPILSKQFRGLVHETSGKFAPVQVSLLHHIVFNPFPFQKFGISSTTSHESS 131
           A T   R  +P+ S +F  L H+ S K  P Q+S++     + F  Q+F  SS+TS ES 
Sbjct: 22  AVTLPARNQTPVFSSRFHSLAHDFSHKLVPAQMSMM-----DSFALQRFNFSSSTSPESD 81

Query: 132 ENKEGSAKQSKNYSTTKDPTKDENSEVSDQAGELNSTSNSHSTKSKSRQWRRRTIRTAFS 191
           E K        + ++ + PT + N     Q G  + + +S +  +K ++   +   ++ S
Sbjct: 82  EKK---THTEASKTSEEKPTAEAN-----QPGLDSESKDSVTDSAKRKRKGAKGAASSSS 141

Query: 192 DSDSEG-CEQPSMDDLVKSLARKEELLKSKHKEIKKMQDKVLRSYAEMENVKDRTRREAE 251
           +SDSE   ++ S DDLVK +A KEELL  K +EIK+++DKVLR+YAEMENV DRTRR+AE
Sbjct: 142 ESDSESDDDELSADDLVKLVAEKEELLSEKEEEIKQLKDKVLRTYAEMENVMDRTRRDAE 201

Query: 252 NSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIKESEDSSGAVPLLKTLLEGVDMTEK 311
           N+KK+A+QNFAKSLLDVADNLGRASSVVK+SFSK+  SEDS+GA PLLKTLLEGV+MTEK
Sbjct: 202 NTKKYAVQNFAKSLLDVADNLGRASSVVKESFSKLDTSEDSAGAAPLLKTLLEGVEMTEK 261

Query: 312 QLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKPDGTVAIVLKSGYMLYDRVLRPA 371
           QL +VFKK G+EK+DP +EPFDP+RHNAVFQ+ D SKP+GTVA VLKSGY LYDRV+RPA
Sbjct: 262 QLAEVFKKFGMEKYDPINEPFDPNRHNAVFQVPDASKPEGTVAHVLKSGYTLYDRVIRPA 318

Query: 372 EVGVTRAAES 381
           EVGVT+  E+
Sbjct: 322 EVGVTQGGEN 318

[Close]

BLAST of MC11g1004.1 vs. TAIR 10
Match: AT5G55200.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 249.6 bits (636), Expect = 4.1e-66
Identity = 159/330 (48.18%), Postives = 201/330 (60.91%), Query Frame = 0


Query: 63  MSVSRVLFRASTRSLRRSS------------PILSKQFRGLVHETSGKFAPVQVSLLHHI 122
           M VSRVL R S  +  RSS            PI++ +F  LVH T  K   V VSL +H 
Sbjct: 1   MLVSRVLSRVSRSAGLRSSFSSVVTPKRNQIPIVASRFHSLVHGTPNKLVAVPVSLRNHG 60

Query: 123 VFNPFPFQKFGISSTTSHESSENKEGSAKQSKNYSTTKDPTKDENSEVSDQAGELNSTSN 182
             +    Q+FG  S++S E   N+          S T+ P   E SE             
Sbjct: 61  TLDLNVLQRFGFFSSSSAEPKGNE----------SNTEVPKTGETSE------------- 120

Query: 183 SHSTKSKSRQWRRRTIRTAFSDSDSEGCEQPSMDDLVKSLARKEELLKSKHKEIKKMQDK 242
                          +    +       +  S DDLVK ++ KE+LLK + K+I +M+DK
Sbjct: 121 --------------NVEVGKATDAEIDFDDLSRDDLVKLVSEKEDLLKVQQKDIMEMKDK 180

Query: 243 VLRSYAEMENVKDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKIKESED 302
            LR+YAE +N+ DRT R AE++KKFA+QNFA SLLDVADNL RASSVVK+SFSKI  S+D
Sbjct: 181 FLRTYAEQQNLMDRTNRNAESAKKFAVQNFATSLLDVADNLERASSVVKESFSKIDTSKD 240

Query: 303 SSGAVPLLKTLLEGVDMTEKQLVQVFKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKPDG 362
            +GA PLLK LLEGV+MTEKQL +VF+K+G+ K DP +EPF+P+RHNAVFQ+ D SKP G
Sbjct: 241 LAGATPLLKNLLEGVEMTEKQLAEVFRKAGLVKEDPLNEPFNPNRHNAVFQVPDASKPKG 293

Query: 363 TVAIVLKSGYMLYDRVLRPAEVGVTRAAES 381
           T+A VLKSGY LYDRV+RPAEVGVT A E+
Sbjct: 301 TIAHVLKSGYSLYDRVIRPAEVGVTCAVEN 293

[Close]

BLAST of MC11g1004.1 vs. TAIR 10
Match: AT5G17710.2 (Co-chaperone GrpE family protein )

HSP 1 Score: 49.3 bits (116), Expect = 8.1e-06
Identity = 57/240 (23.75%), Postives = 104/240 (43.33%), Query Frame = 0


Query: 146 TTKDPTKDENSEVSDQAGELNSTSNSHSTKSKSRQWRRRTIRTAFSDS------DSEGCE 205
           TT+   +    EV +  G ++  + + S +    +     + TA   S      D+   +
Sbjct: 71  TTETEVESNEPEVQETDGAVDVENENASAEEGEAEEEEAAVITALLKSYKEALADNNEGK 130

Query: 206 QPSMDDLVKSLARKEELLKSK----HKEIKKMQDKVLRSYAEMENVKDRTRREAENSKKF 265
              ++  +KS+  ++ LL  K      E+   +D+++R  A+ +N + RT RE  N    
Sbjct: 131 IAEIEASLKSIEDEKFLLADKVASLSNELSVERDRLIRISADFDNFRKRTERERLNLVSN 190

Query: 266 AIQNFAKSLLDVADNLGRASSVVKDSFSKIKESEDSSGAVPLLKTLLEGVDMTEKQLVQV 325
           A     ++LL V DN  RA S +K       E+E         +++        KQ V++
Sbjct: 191 AQGEVVENLLAVLDNFERAKSQIK------VETEGEEKVTNSYQSIY-------KQFVEI 250

Query: 326 FKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKPDGTVAIVLKSGYMLYDRVLRPAEVGVT 376
               GV   +   + FDP  H A+ +       +G V    + G++L +R+LRP+ V V+
Sbjct: 251 LGSLGVIHVETVGKQFDPMLHEAIMREDSAEYEEGIVLEEYRKGFLLGERLLRPSMVKVS 297

[Close]

BLAST of MC11g1004.1 vs. TAIR 10
Match: AT5G17710.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 48.5 bits (114), Expect = 1.4e-05
Identity = 56/235 (23.83%), Postives = 104/235 (44.26%), Query Frame = 0


Query: 151 TKDENSEVSDQAGELNSTSNSHSTKSKSRQWRRRTIRTAFSDS------DSEGCEQPSMD 210
           T+ E++E  +  G ++  + + S +    +     + TA   S      D+   +   ++
Sbjct: 74  TEVESNEPEETDGAVDVENENASAEEGEAEEEEAAVITALLKSYKEALADNNEGKIAEIE 133

Query: 211 DLVKSLARKEELLKSK----HKEIKKMQDKVLRSYAEMENVKDRTRREAENSKKFAIQNF 270
             +KS+  ++ LL  K      E+   +D+++R  A+ +N + RT RE  N    A    
Sbjct: 134 ASLKSIEDEKFLLADKVASLSNELSVERDRLIRISADFDNFRKRTERERLNLVSNAQGEV 193

Query: 271 AKSLLDVADNLGRASSVVKDSFSKIKESEDSSGAVPLLKTLLEGVDMTEKQLVQVFKKSG 330
            ++LL V DN  RA S +K       E+E         +++        KQ V++    G
Sbjct: 194 VENLLAVLDNFERAKSQIK------VETEGEEKVTNSYQSIY-------KQFVEILGSLG 253

Query: 331 VEKFDPTSEPFDPHRHNAVFQISDGSKPDGTVAIVLKSGYMLYDRVLRPAEVGVT 376
           V   +   + FDP  H A+ +       +G V    + G++L +R+LRP+ V V+
Sbjct: 254 VIHVETVGKQFDPMLHEAIMREDSAEYEEGIVLEEYRKGFLLGERLLRPSMVKVS 295

[Close]

BLAST of MC11g1004.1 vs. TAIR 10
Match: AT1G36390.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 44.3 bits (103), Expect = 2.6e-04
Identity = 74/300 (24.67%), Postives = 126/300 (42.00%), Query Frame = 0


Query: 82  PILSKQFRGLVHETSGKFAPVQVSLLHHIVFNPF------PFQKFGISSTTSHESSENKE 141
           P +S  F      +S K  P++ +L  H   +P       P +KF I S  +H+++ ++E
Sbjct: 9   PSISPCFSFSSPSSSSKTLPLRNNLHFHGRPSPISPLISKPSRKFPIFS--AHQTNNSEE 68

Query: 142 GSAKQSKNYSTTKDPTKDENSEVSDQAGELNSTSNSHSTKSKSRQWRRRTIRTAFSDSDS 201
            ++KQ  +  T     K            LN    S +            I T F   + 
Sbjct: 69  ANSKQQADVKTLIRSYKQ---------ALLNGDETSVT-----------EIETMFCKIEK 128

Query: 202 EGCEQPSMDDLVKSLARKEELLKSKHKEIKKMQDKVLRSYAEMENVKDRTRREAENSKKF 261
              E+  MD  V SL+ K     +  KE+K      +R  A+ +N + +  ++  +++  
Sbjct: 129 ---EKNKMDQKVLSLSMK----IASEKEMK------IRLQADFDNTRKKLDKDRLSTESN 188

Query: 262 AIQNFAKSLLDVADNLGRASSVVKDSFSKIKESEDSSGAVPLLKTLLEGVDMTEKQLVQV 321
           A     KSLL + D+  +A   V+    K K+          + T  +G+    +Q V+V
Sbjct: 189 AKVQILKSLLPIIDSFEKAKLQVRVDTDKEKK----------IDTSYQGI---YRQFVEV 248

Query: 322 FKKSGVEKFDPTSEPFDPHRHNAVFQISDGSKPDGTVAIVLKSGYMLYDRVLRPAEVGVT 376
            +   V       +PFDP  H A+ +    +   G +   L  G++L DRVLRPA+V V+
Sbjct: 249 LRYLRVSVIATVGKPFDPLLHEAISREESEAVKAGIITEELNKGFVLGDRVLRPAKVKVS 260

The following BLAST results are available for this feature:

BLAST of MC11g1004.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LB47	2.4e-79	55.48	GrpE protein homolog 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=Mge2 PE...	[more]
Q9FLP3	5.8e-65	48.18	GrpE protein homolog 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=Mge1 PE...	[more]
A4YJR1	6.6e-29	42.35	Protein GrpE OS=Bradyrhizobium sp. (strain ORS 278) OX=114615 GN=grpE PE=3 SV=1	[more]
B6JCI1	6.6e-29	44.23	Protein GrpE OS=Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC...	[more]
B3Q970	1.1e-28	42.07	Protein GrpE OS=Rhodopseudomonas palustris (strain TIE-1) OX=395960 GN=grpE PE=3...	[more]

BLAST of MC11g1004.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139414.1	2.43e-216	100.00	uncharacterized protein LOC111010351 [Momordica charantia]	[more]
XP_038898199.1	3.92e-162	78.95	grpE protein homolog 2, mitochondrial-like [Benincasa hispida]	[more]
XP_022936267.1	6.29e-156	77.38	grpE protein homolog 2, mitochondrial-like [Cucurbita moschata]	[more]
XP_023536002.1	1.32e-154	77.48	grpE protein homolog 2, mitochondrial-like isoform X2 [Cucurbita pepo subsp. pep...	[more]
KAG7024936.1	5.21e-153	76.33	grpE, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MC11g1004.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CCY7	1.18e-216	100.00	GrpE protein homolog OS=Momordica charantia OX=3673 GN=LOC111010351 PE=3 SV=1	[more]
A0A6J1F7Z2	3.04e-156	77.38	GrpE protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111442938 PE=3 SV=1	[more]
M5WTU3	1.09e-118	63.69	GrpE protein homolog OS=Prunus persica OX=3760 GN=PRUPE_4G223200 PE=3 SV=1	[more]
A0A5E4FCQ4	1.09e-118	63.69	GrpE protein homolog OS=Prunus dulcis OX=3755 GN=ALMOND_2B019776 PE=3 SV=1	[more]
A0A6J5X7K4	4.40e-118	63.69	GrpE protein homolog OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS28266 PE=3 SV=1	[more]

BLAST of MC11g1004.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G26780.1	1.7e-80	55.48	Co-chaperone GrpE family protein	[more]
AT5G55200.1	4.1e-66	48.18	Co-chaperone GrpE family protein	[more]
AT5G17710.2	8.1e-06	23.75	Co-chaperone GrpE family protein	[more]
AT5G17710.1	1.4e-05	23.83	Co-chaperone GrpE family protein	[more]
AT1G36390.1	2.6e-04	24.67	Co-chaperone GrpE family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 210..244
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 160..175
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 124..201
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 124..145
None	No IPR available	PANTHER	PTHR21237:SF37	GRPE PROTEIN HOMOLOG	coord: 96..381
IPR000740	GrpE nucleotide exchange factor	PRINTS	PR00773	GRPEPROTEIN	coord: 328..343 score: 43.36 coord: 232..248 score: 43.93 coord: 355..374 score: 51.09
IPR000740	GrpE nucleotide exchange factor	PFAM	PF01025	GrpE	coord: 210..375 e-value: 5.9E-48 score: 162.7
IPR000740	GrpE nucleotide exchange factor	PANTHER	PTHR21237	GRPE PROTEIN	coord: 96..381
IPR000740	GrpE nucleotide exchange factor	PROSITE	PS01071	GRPE	coord: 331..374
IPR000740	GrpE nucleotide exchange factor	HAMAP	MF_01151	GrpE	coord: 152..376 score: 18.259291
IPR000740	GrpE nucleotide exchange factor	CDD	cd00446	GrpE	coord: 225..374 e-value: 4.97895E-59 score: 186.173
IPR009012	GrpE nucleotide exchange factor, head	GENE3D	2.30.22.10	Head domain of nucleotide exchange factor GrpE	coord: 320..376 e-value: 4.7E-19 score: 70.1
IPR009012	GrpE nucleotide exchange factor, head	SUPERFAMILY	51064	Head domain of nucleotide exchange factor GrpE	coord: 321..376
IPR013805	GrpE nucleotide exchange factor, coiled-coil	GENE3D	3.90.20.20		coord: 206..318 e-value: 2.5E-28 score: 100.0
IPR013805	GrpE nucleotide exchange factor, coiled-coil	SUPERFAMILY	58014	Coiled-coil domain of nucleotide exchange factor GrpE	coord: 212..320

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC11g1004	MC11g1004	gene

The following utr5 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g1004.1-utr5	MC11g1004.1-utr5-MC11:8597683..8597684	utr5

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g1004.1-cds	MC11g1004.1-cds-MC11:8597685..8597978	CDS
MC11g1004.1-cds	MC11g1004.1-cds-MC11:8598889..8599368	CDS
MC11g1004.1-cds	MC11g1004.1-cds-MC11:8599491..8599658	CDS
MC11g1004.1-cds	MC11g1004.1-cds-MC11:8599768..8599896	CDS
MC11g1004.1-cds	MC11g1004.1-cds-MC11:8600164..8600253	CDS

The following utr3 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g1004.1-utr3	MC11g1004.1-utr3-MC11:8600254..8600770	utr3

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC11g1004.1	MC11g1004.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006457	protein folding
biological_process	GO:0030150	protein import into mitochondrial matrix
cellular_component	GO:0001405	PAM complex, Tim23 associated import motor
molecular_function	GO:0000774	adenyl-nucleotide exchange factor activity
molecular_function	GO:0051087	chaperone binding
molecular_function	GO:0042803	protein homodimerization activity
molecular_function	GO:0051082	unfolded protein binding