MC11g0521.1 (mRNA) Bitter gourd (Dali-11) v1

BLAST of MC11g0521.1 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 146.7 bits (369), Expect = 7.3e-34
Identity = 80/157 (50.96%), Postives = 103/157 (65.61%), Query Frame = 0

Query: 375 SFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSLNIHDQHRDMRLDIDNMT 434
           S R R    + E+  ALE I + E + +E       S+F   ++IHD+HRDMRLDIDNM+
Sbjct: 522 STRSREGRRLIEIRNALEMIHRGENVRFE-------SIFYGGVDIHDRHRDMRLDIDNMS 581

Query: 435 YEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTSSVEDLSDVKCCICQEEY 494
           YEELL LEER+G VST LSEE +T+ L +  F S      L +SVE   +  CCICQEEY
Sbjct: 582 YEELLALEERIGNVSTGLSEEEVTKLLKQRKFSS----WRLEASVE---EEPCCICQEEY 641

Query: 495 VSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSA 532
           V GD++G L C H +HV C++QWL +KN CPICK +A
Sbjct: 642 VDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPICKNTA 664

BLAST of MC11g0521.1 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 6.4e-30
Identity = 85/220 (38.64%), Postives = 125/220 (56.82%), Query Frame = 0

Query: 320 SGSSHTSSSLQSRSESHSRHHMT-------YPEHEDYSQSLLDIGQTTAAEGGISSTSTN 379
           +G S +S+ +   S S+SR H +         E ++    L  IG++ AA+G        
Sbjct: 499 AGPSVSSNEVTMPSSSNSRSHRSRHRRSGLLLERQNELLHLRHIGRSLAADG-------- 558

Query: 380 LNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSLNIHDQHRDMRLDIDN 439
             + R + +  I +VL A+ R    E L  E  ++ +  ++    ++HD+HR+MRLD+DN
Sbjct: 559 --NGRNQIISEIRQVLHAMRR---GENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDVDN 618

Query: 440 MTYEELLDLEERMGTVSTALSEEALTECLNR-SIFQSKPQGGELTSSVEDLSDVKCCICQ 499
           M+YEELL L ER+G VST LSEE + + + +     S P   EL  ++E      CCICQ
Sbjct: 619 MSYEELLALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIE-----PCCICQ 678

Query: 500 EEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSA 532
           EEYV GD +G L C H++H  CI+QW+ +KN CPICKT A
Sbjct: 679 EEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTEA 700

BLAST of MC11g0521.1 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 1.1e-29
Identity = 84/219 (38.36%), Postives = 122/219 (55.71%), Query Frame = 0

Query: 320 SGSSHTSSSLQSRSESHSRHHMTYP-------EHEDYSQSLLDIGQTTAAEGGISSTSTN 379
           +G S +S+   + S S SR H +         E ++    L  +G++ AA+         
Sbjct: 465 TGPSVSSNEAAAPSGSSSRSHRSRQRRSGLLLERQNDHLHLRHLGRSLAAD--------- 524

Query: 380 LNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSLNIHDQHRDMRLDIDN 439
            N  R R +  I +VL A+ R    E L +E  ++ +  ++     +HD+HRDMRLD+DN
Sbjct: 525 -NDGRNRLISEIRQVLSAMRR---GENLRFEDYMVFDPLIYQGMAEMHDRHRDMRLDVDN 584

Query: 440 MTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTSSVEDLSDVKCCICQE 499
           M+YEELL L ER+G VST LSEE + + + +    S   G     S +D+    CC+CQE
Sbjct: 585 MSYEELLALGERIGDVSTGLSEEVILKVMKQHKHTSSAAG-----SHQDME--PCCVCQE 644

Query: 500 EYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSA 532
           EY  GD++G L C H++H  C++QWL LKN CPICKT A
Sbjct: 645 EYAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPICKTVA 663

BLAST of MC11g0521.1 vs. ExPASy Swiss-Prot
Match: Q5QLR5 (Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZFP1 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.9e-26
Identity = 61/129 (47.29%), Postives = 82/129 (63.57%), Query Frame = 0

Query: 403 EQAILLETSLFLSSLNIHDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLN 462
           E A +++ S      N+ D+HRDMRLDID+MTYEELL LEE++G V+T L++  + E L 
Sbjct: 387 EHATIMDFSRLYEVSNVVDEHRDMRLDIDSMTYEELLALEEQIGDVNTGLAKSYIVEKLK 446

Query: 463 RSIFQSKPQGGELTSSVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKN 522
            S+F          SS   + +  C ICQEEY   + +G L C H+YH  CI+QWL +KN
Sbjct: 447 TSLFVPGSSCMSNKSSESSMENDACIICQEEYQVKECIGTLDCGHRYHEDCIKQWLMVKN 506

Query: 523 WCPICKTSA 532
            CPICKT+A
Sbjct: 507 LCPICKTTA 515

BLAST of MC11g0521.1 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.4e-21
Identity = 149/560 (26.61%), Postives = 227/560 (40.54%), Query Frame = 0

Query: 19  PGVVLRDNMKNRDHSNHGSRLGCTGRI-------CSSSGAEVGCSSKASSKRP----FRS 78
           PG    +N      S  G R  C  +         SS G       ++SS  P    FR 
Sbjct: 159 PGRSFEENGVGTGSSVEGRRASCKRKALEGSISQSSSGGYHDFQRGESSSWTPGSTVFRP 218

Query: 79  SSGKEIVGSSSR------SSSIRNFRKSFPD----PFGKLSSKLETDSSEDGSIQDELEE 138
            +G  I GS         S ++ NF  S P+       + SS+     S     Q+ +  
Sbjct: 219 GNGLNISGSLDNGPRGMVSGTVPNFPVSAPNFPVSAIAESSSRNICVRSNPSDHQETVNP 278

Query: 139 LEFIS--------PPGMFHTVLHVKSESSV----PADAILMERGSSSTD-SNTKSRRNSI 198
             F +        PP   +   H+ ++       P  + ++ R  +ST  S     R  +
Sbjct: 279 STFAAGTVVRRPVPPSQLNLSRHLPADQHSLDLRPGQSFVVSRNPNSTPVSIPPGSRTML 338

Query: 199 QRYGVDNQDTSTSMRSRSLCQALNGGTSRY---VSRNLECNSV-SEIIP------PDSSS 258
             +             R    +L GGTS     V RNL  +   S  IP      P  ++
Sbjct: 339 PPF-------------RWTGSSLVGGTSNSTAPVERNLHLDETRSRSIPGNTLEIPMFAA 398

Query: 259 KEQNLVRRKNVTKKMIYDGENSSSSRGKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQ 318
            E     R   ++ +     NS+SS  +  S TS          ++ +  +   +   R+
Sbjct: 399 PEVGNFARSQSSRNVTNGNLNSASSVSRTGSTTSVPPPPPPSSNLAWTSYQNSPHYQRRR 458

Query: 319 NVLSSLGTRSLASACDGASHSYQGISGDRSLQDSLMTSWMPQSNISGSSHTSSSLQSRSE 378
              S L  RSL S+   A+ +    SGD     SL            +S     LQ   +
Sbjct: 459 TERSELARRSLLSSL--AADATNQRSGDHPTLRSLAPP---------ASSDGLVLQPGGD 518

Query: 379 SHSRHHMTY----PEHEDYSQSLLDIGQTTAAEGGISSTSTNLNSFRCRNLDGIAEVLLA 438
           +   H+  Y    P  +    S++ I     A    S   + L   + +N      VL  
Sbjct: 519 NSQMHNRAYSRAGPLFDRQGDSVVGIPHPLRALAAASRGRSRLMVSQMQN------VLDV 578

Query: 439 LERIEQEEELTYEQAILLETSLFLSSLNIHDQHRDMRLDIDNMTYEELLDLEERMGTVST 498
           + R      L  E  +LL  S+       HD++RDMRLD+DNM+YEELL LEER+G V T
Sbjct: 579 MRRDANNNNLRLEDVMLLNHSVLFDGATGHDRYRDMRLDVDNMSYEELLALEERIGDVCT 638

Query: 499 ALSEEALTECLNRSIFQSKPQGGELTSSVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYH 531
            ++EE ++  L +  ++S       T S +D     CC+CQEEY  G+++G L C H++H
Sbjct: 639 GVNEETISNRLKQRKYKSN------TKSPQDAE--PCCVCQEEYTEGEDMGTLECGHEFH 680

BLAST of MC11g0521.1 vs. NCBI nr
Match: XP_022136854.1 (probable E3 ubiquitin-protein ligase RHG1A [Momordica charantia])

HSP 1 Score: 1044 bits (2699), Expect = 0.0
Identity = 538/538 (100.00%), Postives = 538/538 (100.00%), Query Frame = 0

Query: 1   MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSNHGSRLGCTGRICSSSGAEVGCSSKAS 60
           MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSNHGSRLGCTGRICSSSGAEVGCSSKAS
Sbjct: 1   MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSNHGSRLGCTGRICSSSGAEVGCSSKAS 60

Query: 61  SKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEELEF 120
           SKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEELEF
Sbjct: 61  SKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEELEF 120

Query: 121 ISPPGMFHTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSMRS 180
           ISPPGMFHTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSMRS
Sbjct: 121 ISPPGMFHTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSMRS 180

Query: 181 RSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGENSSSSR 240
           RSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGENSSSSR
Sbjct: 181 RSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGENSSSSR 240

Query: 241 GKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHSYQGIS 300
           GKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHSYQGIS
Sbjct: 241 GKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHSYQGIS 300

Query: 301 GDRSLQDSLMTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYSQSLLDIGQTT 360
           GDRSLQDSLMTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYSQSLLDIGQTT
Sbjct: 301 GDRSLQDSLMTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYSQSLLDIGQTT 360

Query: 361 AAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSLNIH 420
           AAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSLNIH
Sbjct: 361 AAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSLNIH 420

Query: 421 DQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTSSVE 480
           DQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTSSVE
Sbjct: 421 DQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTSSVE 480

Query: 481 DLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSAHQLHPFK 538
           DLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSAHQLHPFK
Sbjct: 481 DLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSAHQLHPFK 538

BLAST of MC11g0521.1 vs. NCBI nr
Match: XP_023524149.1 (uncharacterized protein LOC111788150 [Cucurbita pepo subsp. pepo] >XP_023524150.1 uncharacterized protein LOC111788150 [Cucurbita pepo subsp. pepo] >XP_023524151.1 uncharacterized protein LOC111788150 [Cucurbita pepo subsp. pepo] >XP_023524152.1 uncharacterized protein LOC111788150 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 799 bits (2064), Expect = 3.86e-287
Identity = 425/543 (78.27%), Postives = 468/543 (86.19%), Query Frame = 0

Query: 1   MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSN-HGSRLGCTGRICSSSGAEVGCSSKA 60
           MDEYSS+RVH+GIVSRKGPGVVLRDN+KNRD SN +G+RLGCTGR+C++SG +VGCS+K 
Sbjct: 1   MDEYSSKRVHMGIVSRKGPGVVLRDNVKNRDQSNRYGNRLGCTGRVCTTSGVQVGCSNKT 60

Query: 61  SSKRPFRSSSGKEIVGSSSRSSS-IRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEEL 120
           SSKRPFRSSSGKE++GSSS+SSS IRN RKSFP+PF KLSSKLETDSSE+ SIQD+LEEL
Sbjct: 61  SSKRPFRSSSGKEVLGSSSKSSSAIRNLRKSFPEPFAKLSSKLETDSSENDSIQDDLEEL 120

Query: 121 EFISPPGMFHTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSM 180
           EFISPP +FHT L VKSESS+PAD +LMERGS STD+NT+S+RNS+QR+ VDNQD+  SM
Sbjct: 121 EFISPPVLFHTGLQVKSESSLPADTLLMERGSCSTDANTESKRNSVQRFEVDNQDSHASM 180

Query: 181 RSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGENSSS 240
           + RSLCQ  NGG SRY SR+L C+SVSEIIP D S  E+NLVRRKNVTKKMIYDGENSS 
Sbjct: 181 QPRSLCQTSNGGNSRYSSRSLPCDSVSEIIPSDGSLLERNLVRRKNVTKKMIYDGENSSH 240

Query: 241 SRGKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHSYQG 300
           SRGKKV GTS GQNC  R GI ISDQR GRN+FHRQNVLSS GTRSL S C GA HSYQ 
Sbjct: 241 SRGKKVGGTSRGQNCVSRHGIFISDQRPGRNVFHRQNVLSSYGTRSLTSVCSGARHSYQS 300

Query: 301 ISGDRSLQDSL-MTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYSQSLLDIG 360
            S + S QDSL M SWMPQSNIS SS+  SSLQS SESHSRH MTYPE EDYSQS+ DIG
Sbjct: 301 NSDNLSSQDSLAMNSWMPQSNISSSSNAFSSLQSSSESHSRHRMTYPEPEDYSQSIHDIG 360

Query: 361 QTTAAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSL 420
           + T  E  ISST TNLNSFRC N  GIAEVLLALERIEQEEELTYEQAILLETS+FL+SL
Sbjct: 361 RATPTEADISSTLTNLNSFRCHN--GIAEVLLALERIEQEEELTYEQAILLETSMFLNSL 420

Query: 421 NIHDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTS 480
           NI+DQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECL+ SI+QSKP G   T 
Sbjct: 421 NINDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLDTSIYQSKPGGRAATG 480

Query: 481 SVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSA--HQLH 538
           S+E+LSD+KCCICQEEY+ GDEVGRL CEHKYHV CIQQWLRLKNWCPICK SA   QLH
Sbjct: 481 SIEELSDIKCCICQEEYLIGDEVGRLQCEHKYHVACIQQWLRLKNWCPICKASAASQQLH 540

BLAST of MC11g0521.1 vs. NCBI nr
Match: XP_022940547.1 (uncharacterized protein LOC111446115 [Cucurbita moschata] >XP_022940548.1 uncharacterized protein LOC111446115 [Cucurbita moschata] >XP_022940549.1 uncharacterized protein LOC111446115 [Cucurbita moschata])

HSP 1 Score: 797 bits (2059), Expect = 2.22e-286
Identity = 426/543 (78.45%), Postives = 465/543 (85.64%), Query Frame = 0

Query: 1   MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSN-HGSRLGCTGRICSSSGAEVGCSSKA 60
           MDEYSS+RVH+GIVSRKGPGVVLRDN+KNRD SN +G+RLGCTGRIC++SG +VGCS+K 
Sbjct: 1   MDEYSSKRVHMGIVSRKGPGVVLRDNVKNRDQSNRYGNRLGCTGRICTTSGVQVGCSNKT 60

Query: 61  SSKRPFRSSSGKEIVGSSSRSSS-IRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEEL 120
           SSKRPFRSSSGKE+VGSSS+SSS IRN RKSFP+PF KLSSKLETDSSE+ SIQD+LEEL
Sbjct: 61  SSKRPFRSSSGKEVVGSSSKSSSAIRNLRKSFPEPFAKLSSKLETDSSENDSIQDDLEEL 120

Query: 121 EFISPPGMFHTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSM 180
           EFISPP +FHT L VKSESS+PA+  LMERGS STD+NT+S+RNS+QR+ VDNQD+  SM
Sbjct: 121 EFISPPVLFHTGLQVKSESSLPANTSLMERGSCSTDANTESKRNSVQRFEVDNQDSHASM 180

Query: 181 RSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGENSSS 240
           + RSLCQ  NGG SRY SRN  C+SVSEIIP D S  E+NLVRRKNVTKKMIYDGENSS 
Sbjct: 181 QPRSLCQTSNGGNSRYSSRNFPCDSVSEIIPSDGSLLERNLVRRKNVTKKMIYDGENSSH 240

Query: 241 SRGKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHSYQG 300
           SRGKKV GTS GQNC  R GI ISDQR GRN+FHRQNVLSS GTRSL S C GA HSYQ 
Sbjct: 241 SRGKKVGGTSRGQNCVSRHGIFISDQRPGRNVFHRQNVLSSYGTRSLTSVCSGARHSYQS 300

Query: 301 ISGDRSLQDSL-MTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYSQSLLDIG 360
            S + S QDSL M SWMPQSNIS SS+  SSLQS SESHSRH M YPE EDYSQS+ DIG
Sbjct: 301 NSDNLSSQDSLAMNSWMPQSNISSSSNAFSSLQSSSESHSRHRMIYPEPEDYSQSIHDIG 360

Query: 361 QTTAAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSL 420
           + T  E  ISST TNLNSFRC N  GIAEVLLALERIEQEEELTYEQAILLETS+FL+SL
Sbjct: 361 RATPTEADISSTLTNLNSFRCHN--GIAEVLLALERIEQEEELTYEQAILLETSMFLNSL 420

Query: 421 NIHDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTS 480
           NI+DQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLN SI+QSKP G   T 
Sbjct: 421 NINDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNTSIYQSKPGGRAATG 480

Query: 481 SVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSA--HQLH 538
           S+E+LSD+KCCICQEEY+ GDEVGRL CEHKYHV CIQQWLRLKNWCPICK SA   QLH
Sbjct: 481 SIEELSDIKCCICQEEYLIGDEVGRLQCEHKYHVACIQQWLRLKNWCPICKASAASQQLH 540

BLAST of MC11g0521.1 vs. NCBI nr
Match: KAG7037563.1 (putative E3 ubiquitin-protein ligase HIP1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 788 bits (2036), Expect = 6.06e-283
Identity = 422/542 (77.86%), Postives = 461/542 (85.06%), Query Frame = 0

Query: 1   MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSN-HGSRLGCTGRICSSSGAEVGCSSKA 60
           MDEYSS+RVH+GIVSRKGPGVVLRDN+KNRD SN +G+RLGCTGRIC++SG +VGCS+K 
Sbjct: 1   MDEYSSKRVHMGIVSRKGPGVVLRDNVKNRDQSNRYGNRLGCTGRICTTSGVQVGCSNKT 60

Query: 61  SSKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEELE 120
           SSKRPFRSSSGKE+VGSSS   +I N RKSFP+PF KLSSKLETDSSE+ SIQD+LEELE
Sbjct: 61  SSKRPFRSSSGKEVVGSSS---AIINLRKSFPEPFAKLSSKLETDSSENDSIQDDLEELE 120

Query: 121 FISPPGMFHTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSMR 180
           FISPP +FHT L VKSESS+PA+  LMERGS STD+NT+S+RNS+QR+ VDNQD+  SM+
Sbjct: 121 FISPPVLFHTGLQVKSESSLPANTSLMERGSCSTDANTESKRNSVQRFEVDNQDSHASMQ 180

Query: 181 SRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGENSSSS 240
            RSLCQ  NGG SRY SRN  C+SVSEIIP   S  E+NLVRRKNVTKKMIYDGENSS S
Sbjct: 181 PRSLCQTSNGGNSRYSSRNFPCDSVSEIIPSYGSLLERNLVRRKNVTKKMIYDGENSSHS 240

Query: 241 RGKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHSYQGI 300
           RGKKV GTS GQNC  R GI ISDQR GRN+FHRQNVLSS GTRSL S C GA HSYQ  
Sbjct: 241 RGKKVGGTSRGQNCVSRHGIFISDQRPGRNVFHRQNVLSSYGTRSLTSVCSGARHSYQSN 300

Query: 301 SGDRSLQDSL-MTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYSQSLLDIGQ 360
           S + S QDSL M SWMPQSNIS SS+  SSLQS SESHSRH MTYPE EDYSQS+ DIG+
Sbjct: 301 SDNLSSQDSLAMNSWMPQSNISSSSNAFSSLQSSSESHSRHRMTYPEPEDYSQSIHDIGR 360

Query: 361 TTAAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSLN 420
            T  E  ISST TNLNSFRC N  GIAEVLLALERIEQEEELTYEQAILLETS+FL+SLN
Sbjct: 361 ATPTEADISSTLTNLNSFRCHN--GIAEVLLALERIEQEEELTYEQAILLETSMFLNSLN 420

Query: 421 IHDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTSS 480
           I+DQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLN SI+QSKP G   T S
Sbjct: 421 IYDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNTSIYQSKPGGRAATGS 480

Query: 481 VEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSA--HQLHP 538
           +E+LSD+KCCICQEEY+ GDEVGRL CEHKYHV CIQQWLRLKNWCPICK SA   QLHP
Sbjct: 481 IEELSDIKCCICQEEYLIGDEVGRLQCEHKYHVACIQQWLRLKNWCPICKASAASQQLHP 537

BLAST of MC11g0521.1 vs. NCBI nr
Match: XP_022981972.1 (uncharacterized protein LOC111480963 [Cucurbita maxima] >XP_022981973.1 uncharacterized protein LOC111480963 [Cucurbita maxima] >XP_022981974.1 uncharacterized protein LOC111480963 [Cucurbita maxima] >XP_022981975.1 uncharacterized protein LOC111480963 [Cucurbita maxima] >XP_022981976.1 uncharacterized protein LOC111480963 [Cucurbita maxima])

HSP 1 Score: 787 bits (2033), Expect = 2.01e-282
Identity = 420/543 (77.35%), Postives = 462/543 (85.08%), Query Frame = 0

Query: 1   MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSN-HGSRLGCTGRICSSSGAEVGCSSKA 60
           MDEY+S+RVH+GIVSRKGPGVVLRDN+KNRD SN +G+RLGCTGRIC++SG +VGCS+K 
Sbjct: 1   MDEYASKRVHMGIVSRKGPGVVLRDNVKNRDQSNRYGNRLGCTGRICTTSGVQVGCSNKT 60

Query: 61  SSKRPFRSSSGKEIVGSSSRSSS-IRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEEL 120
           SSKRPFRSSSGKE+VGSSS+SSS IRN RKSFP+PF KLSSKLETDSSE+ SIQD+LEEL
Sbjct: 61  SSKRPFRSSSGKEVVGSSSKSSSAIRNLRKSFPEPFAKLSSKLETDSSENDSIQDDLEEL 120

Query: 121 EFISPPGMFHTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSM 180
           EFISPP +FHT L VKSESS+PAD +LMERG  STD+NT+S+RNS+QR+ VD QD+  SM
Sbjct: 121 EFISPPVLFHTGLQVKSESSLPADTLLMERGGCSTDANTESKRNSVQRFEVDCQDSHASM 180

Query: 181 RSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGENSSS 240
           + RSLCQ  NGG SRY SRNL C+SVSEIIP D S  E+NLVRRKNVTKKMIYDGENSS 
Sbjct: 181 QPRSLCQTSNGGNSRYSSRNLPCDSVSEIIPSDGSLLERNLVRRKNVTKKMIYDGENSSH 240

Query: 241 SRGKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHSYQG 300
           SRGKKV GTS GQNC  R GI ISDQR GRN+FHRQNVLSS GTRSL S C GA HSYQ 
Sbjct: 241 SRGKKVGGTSQGQNCVSRHGIFISDQRPGRNVFHRQNVLSSYGTRSLTSVCSGARHSYQS 300

Query: 301 ISGDRSLQDSL-MTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYSQSLLDIG 360
            S + SL+DSL + SWMPQSNIS SS+  SSLQS SESHSRH MTYPE EDYSQS+ DIG
Sbjct: 301 NSDNLSLEDSLALNSWMPQSNISSSSNAFSSLQSSSESHSRHRMTYPEPEDYSQSIHDIG 360

Query: 361 QTTAAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSL 420
           + T  E  ISST TNLNSFRC N  GIAEVLLALERIEQEEELTYEQAILLETS+FL+SL
Sbjct: 361 RATPTEVDISSTLTNLNSFRCHN--GIAEVLLALERIEQEEELTYEQAILLETSMFLNSL 420

Query: 421 NIHDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTS 480
           NI+DQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEE LTECLN SI+Q KP G     
Sbjct: 421 NINDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEVLTECLNTSIYQPKPGGRAAMG 480

Query: 481 SVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSA--HQLH 538
           S+E+LSD+KCCICQEEY+ GD VGRL CEHKYHV CIQQWLRLKNWCPICK SA   QLH
Sbjct: 481 SIEELSDIKCCICQEEYLIGDNVGRLQCEHKYHVACIQQWLRLKNWCPICKASAASQQLH 540

BLAST of MC11g0521.1 vs. ExPASy TrEMBL
Match: A0A6J1C5H9 (probable E3 ubiquitin-protein ligase RHG1A OS=Momordica charantia OX=3673 GN=LOC111008445 PE=4 SV=1)

HSP 1 Score: 1044 bits (2699), Expect = 0.0
Identity = 538/538 (100.00%), Postives = 538/538 (100.00%), Query Frame = 0

Query: 1   MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSNHGSRLGCTGRICSSSGAEVGCSSKAS 60
           MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSNHGSRLGCTGRICSSSGAEVGCSSKAS
Sbjct: 1   MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSNHGSRLGCTGRICSSSGAEVGCSSKAS 60

Query: 61  SKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEELEF 120
           SKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEELEF
Sbjct: 61  SKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEELEF 120

Query: 121 ISPPGMFHTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSMRS 180
           ISPPGMFHTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSMRS
Sbjct: 121 ISPPGMFHTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSMRS 180

Query: 181 RSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGENSSSSR 240
           RSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGENSSSSR
Sbjct: 181 RSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGENSSSSR 240

Query: 241 GKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHSYQGIS 300
           GKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHSYQGIS
Sbjct: 241 GKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHSYQGIS 300

Query: 301 GDRSLQDSLMTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYSQSLLDIGQTT 360
           GDRSLQDSLMTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYSQSLLDIGQTT
Sbjct: 301 GDRSLQDSLMTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYSQSLLDIGQTT 360

Query: 361 AAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSLNIH 420
           AAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSLNIH
Sbjct: 361 AAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSLNIH 420

Query: 421 DQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTSSVE 480
           DQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTSSVE
Sbjct: 421 DQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTSSVE 480

Query: 481 DLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSAHQLHPFK 538
           DLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSAHQLHPFK
Sbjct: 481 DLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSAHQLHPFK 538

BLAST of MC11g0521.1 vs. ExPASy TrEMBL
Match: A0A6J1FKJ9 (uncharacterized protein LOC111446115 OS=Cucurbita moschata OX=3662 GN=LOC111446115 PE=4 SV=1)

HSP 1 Score: 797 bits (2059), Expect = 1.08e-286
Identity = 426/543 (78.45%), Postives = 465/543 (85.64%), Query Frame = 0

Query: 1   MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSN-HGSRLGCTGRICSSSGAEVGCSSKA 60
           MDEYSS+RVH+GIVSRKGPGVVLRDN+KNRD SN +G+RLGCTGRIC++SG +VGCS+K 
Sbjct: 1   MDEYSSKRVHMGIVSRKGPGVVLRDNVKNRDQSNRYGNRLGCTGRICTTSGVQVGCSNKT 60

Query: 61  SSKRPFRSSSGKEIVGSSSRSSS-IRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEEL 120
           SSKRPFRSSSGKE+VGSSS+SSS IRN RKSFP+PF KLSSKLETDSSE+ SIQD+LEEL
Sbjct: 61  SSKRPFRSSSGKEVVGSSSKSSSAIRNLRKSFPEPFAKLSSKLETDSSENDSIQDDLEEL 120

Query: 121 EFISPPGMFHTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSM 180
           EFISPP +FHT L VKSESS+PA+  LMERGS STD+NT+S+RNS+QR+ VDNQD+  SM
Sbjct: 121 EFISPPVLFHTGLQVKSESSLPANTSLMERGSCSTDANTESKRNSVQRFEVDNQDSHASM 180

Query: 181 RSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGENSSS 240
           + RSLCQ  NGG SRY SRN  C+SVSEIIP D S  E+NLVRRKNVTKKMIYDGENSS 
Sbjct: 181 QPRSLCQTSNGGNSRYSSRNFPCDSVSEIIPSDGSLLERNLVRRKNVTKKMIYDGENSSH 240

Query: 241 SRGKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHSYQG 300
           SRGKKV GTS GQNC  R GI ISDQR GRN+FHRQNVLSS GTRSL S C GA HSYQ 
Sbjct: 241 SRGKKVGGTSRGQNCVSRHGIFISDQRPGRNVFHRQNVLSSYGTRSLTSVCSGARHSYQS 300

Query: 301 ISGDRSLQDSL-MTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYSQSLLDIG 360
            S + S QDSL M SWMPQSNIS SS+  SSLQS SESHSRH M YPE EDYSQS+ DIG
Sbjct: 301 NSDNLSSQDSLAMNSWMPQSNISSSSNAFSSLQSSSESHSRHRMIYPEPEDYSQSIHDIG 360

Query: 361 QTTAAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSL 420
           + T  E  ISST TNLNSFRC N  GIAEVLLALERIEQEEELTYEQAILLETS+FL+SL
Sbjct: 361 RATPTEADISSTLTNLNSFRCHN--GIAEVLLALERIEQEEELTYEQAILLETSMFLNSL 420

Query: 421 NIHDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTS 480
           NI+DQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLN SI+QSKP G   T 
Sbjct: 421 NINDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNTSIYQSKPGGRAATG 480

Query: 481 SVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSA--HQLH 538
           S+E+LSD+KCCICQEEY+ GDEVGRL CEHKYHV CIQQWLRLKNWCPICK SA   QLH
Sbjct: 481 SIEELSDIKCCICQEEYLIGDEVGRLQCEHKYHVACIQQWLRLKNWCPICKASAASQQLH 540

BLAST of MC11g0521.1 vs. ExPASy TrEMBL
Match: A0A6J1J3B4 (uncharacterized protein LOC111480963 OS=Cucurbita maxima OX=3661 GN=LOC111480963 PE=4 SV=1)

HSP 1 Score: 787 bits (2033), Expect = 9.72e-283
Identity = 420/543 (77.35%), Postives = 462/543 (85.08%), Query Frame = 0

Query: 1   MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSN-HGSRLGCTGRICSSSGAEVGCSSKA 60
           MDEY+S+RVH+GIVSRKGPGVVLRDN+KNRD SN +G+RLGCTGRIC++SG +VGCS+K 
Sbjct: 1   MDEYASKRVHMGIVSRKGPGVVLRDNVKNRDQSNRYGNRLGCTGRICTTSGVQVGCSNKT 60

Query: 61  SSKRPFRSSSGKEIVGSSSRSSS-IRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEEL 120
           SSKRPFRSSSGKE+VGSSS+SSS IRN RKSFP+PF KLSSKLETDSSE+ SIQD+LEEL
Sbjct: 61  SSKRPFRSSSGKEVVGSSSKSSSAIRNLRKSFPEPFAKLSSKLETDSSENDSIQDDLEEL 120

Query: 121 EFISPPGMFHTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSM 180
           EFISPP +FHT L VKSESS+PAD +LMERG  STD+NT+S+RNS+QR+ VD QD+  SM
Sbjct: 121 EFISPPVLFHTGLQVKSESSLPADTLLMERGGCSTDANTESKRNSVQRFEVDCQDSHASM 180

Query: 181 RSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGENSSS 240
           + RSLCQ  NGG SRY SRNL C+SVSEIIP D S  E+NLVRRKNVTKKMIYDGENSS 
Sbjct: 181 QPRSLCQTSNGGNSRYSSRNLPCDSVSEIIPSDGSLLERNLVRRKNVTKKMIYDGENSSH 240

Query: 241 SRGKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHSYQG 300
           SRGKKV GTS GQNC  R GI ISDQR GRN+FHRQNVLSS GTRSL S C GA HSYQ 
Sbjct: 241 SRGKKVGGTSQGQNCVSRHGIFISDQRPGRNVFHRQNVLSSYGTRSLTSVCSGARHSYQS 300

Query: 301 ISGDRSLQDSL-MTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYSQSLLDIG 360
            S + SL+DSL + SWMPQSNIS SS+  SSLQS SESHSRH MTYPE EDYSQS+ DIG
Sbjct: 301 NSDNLSLEDSLALNSWMPQSNISSSSNAFSSLQSSSESHSRHRMTYPEPEDYSQSIHDIG 360

Query: 361 QTTAAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSL 420
           + T  E  ISST TNLNSFRC N  GIAEVLLALERIEQEEELTYEQAILLETS+FL+SL
Sbjct: 361 RATPTEVDISSTLTNLNSFRCHN--GIAEVLLALERIEQEEELTYEQAILLETSMFLNSL 420

Query: 421 NIHDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTS 480
           NI+DQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEE LTECLN SI+Q KP G     
Sbjct: 421 NINDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEVLTECLNTSIYQPKPGGRAAMG 480

Query: 481 SVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSA--HQLH 538
           S+E+LSD+KCCICQEEY+ GD VGRL CEHKYHV CIQQWLRLKNWCPICK SA   QLH
Sbjct: 481 SIEELSDIKCCICQEEYLIGDNVGRLQCEHKYHVACIQQWLRLKNWCPICKASAASQQLH 540

BLAST of MC11g0521.1 vs. ExPASy TrEMBL
Match: A0A6J1FF39 (uncharacterized RING finger protein P4H10.07-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443372 PE=4 SV=1)

HSP 1 Score: 753 bits (1944), Expect = 2.15e-269
Identity = 410/540 (75.93%), Postives = 457/540 (84.63%), Query Frame = 0

Query: 1   MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSN-HGSRLGCTGRICSSSGAEVGCSSKA 60
           MDEY S+RVHVGIVSRKGPGVVLRDNMK RD SN H  RLGCTGRICSSS A+VG S+KA
Sbjct: 1   MDEYCSKRVHVGIVSRKGPGVVLRDNMKQRDQSNRHSDRLGCTGRICSSSDAQVGRSNKA 60

Query: 61  SSKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEELE 120
           SSKR FRSSSGKE+VGSSS   +IRNFR SFP+PFGKLSSKLETDSS++ SIQDELEE+E
Sbjct: 61  SSKRLFRSSSGKEVVGSSS---AIRNFRNSFPEPFGKLSSKLETDSSDNDSIQDELEEME 120

Query: 121 FISPPGMFHTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSMR 180
           F SPPG+ HT LHVKSE SVPADA+LMERGS S +SN KS+RN+IQRY VDNQ+T  SM 
Sbjct: 121 FTSPPGLLHTGLHVKSERSVPADAMLMERGSCSMNSNNKSKRNTIQRYKVDNQETHASMW 180

Query: 181 SRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGENSSSS 240
            RSLCQ  NGG+SR+ SRNL C+SVSEI+  DSS  +QNLVRRKNVTKKMIYDGENSS+S
Sbjct: 181 PRSLCQISNGGSSRFDSRNLACDSVSEIVSSDSSLLDQNLVRRKNVTKKMIYDGENSSNS 240

Query: 241 RGKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHSYQGI 300
           R K VSGTS GQ C YR GISISDQR GRN+FH+QNVLSS GTRS  S C GA HS QGI
Sbjct: 241 REKIVSGTSRGQKCSYRHGISISDQRPGRNVFHKQNVLSSHGTRSWTSGCGGARHS-QGI 300

Query: 301 SGDRSLQDSL-MTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYSQSLLDIGQ 360
           S + S+ DSL MTSW P++++S SS+ SSSLQ  SESHSRH MTYPE E YS+SL     
Sbjct: 301 SDNLSVHDSLTMTSWTPRTSVSSSSNASSSLQLSSESHSRHRMTYPEPEGYSRSLH---- 360

Query: 361 TTAAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSLN 420
             AAE GIS+TSTN+NSFRCRN++GIAEVLLALERIE+EE  TYEQAILLETS FL+ LN
Sbjct: 361 --AAEAGISNTSTNVNSFRCRNVNGIAEVLLALERIEREER-TYEQAILLETSTFLNRLN 420

Query: 421 IHDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTSS 480
           I+DQHRDMRLDIDNMTYE+LLDLEE MGTVSTALSE+ LTECL+RSI+QSKP+GG  T S
Sbjct: 421 IYDQHRDMRLDIDNMTYEQLLDLEEGMGTVSTALSEKELTECLHRSIYQSKPEGGTATGS 480

Query: 481 VEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSAHQLHPFK 538
           VEDLSDVKCCICQEEY SGDEVGRL CEHKYHV CIQQWLR+KNWCPICK++A  L PF+
Sbjct: 481 VEDLSDVKCCICQEEYASGDEVGRLQCEHKYHVACIQQWLRMKNWCPICKSTA--LSPFQ 527

BLAST of MC11g0521.1 vs. ExPASy TrEMBL
Match: A0A6J1F8U9 (uncharacterized RING finger protein P4H10.07-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443372 PE=4 SV=1)

HSP 1 Score: 746 bits (1927), Expect = 7.99e-267
Identity = 409/540 (75.74%), Postives = 456/540 (84.44%), Query Frame = 0

Query: 1   MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSN-HGSRLGCTGRICSSSGAEVGCSSKA 60
           MDEY S+RVHVGIVSRKGPGVVLRDNMK RD SN H  RLGCTGRICSSS A+VG S+KA
Sbjct: 1   MDEYCSKRVHVGIVSRKGPGVVLRDNMKQRDQSNRHSDRLGCTGRICSSSDAQVGRSNKA 60

Query: 61  SSKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEELE 120
           SSKR FRSSSGKE+VGSSS   +IRNFR SFP+PFGKLSSKLETDSS++ SIQDELEE+E
Sbjct: 61  SSKRLFRSSSGKEVVGSSS---AIRNFRNSFPEPFGKLSSKLETDSSDNDSIQDELEEME 120

Query: 121 FISPPGMFHTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSMR 180
           F SPPG+ HT LHVKSE SVPADA+LMERGS S +SN KS+RN+IQRY VDNQ+T  SM 
Sbjct: 121 FTSPPGLLHTGLHVKSERSVPADAMLMERGSCSMNSNNKSKRNTIQRYKVDNQETHASMW 180

Query: 181 SRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGENSSSS 240
            RSLCQ  NGG+SR+ SRNL C+SVSEI+  DSS  +QNLVRRKNVTKKMIYDGENSS+S
Sbjct: 181 PRSLCQISNGGSSRFDSRNLACDSVSEIVSSDSSLLDQNLVRRKNVTKKMIYDGENSSNS 240

Query: 241 RGKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHSYQGI 300
           R K VSGTS GQ C YR GISISDQR GRN+FH+QNVLSS GTRS  S C GA HS QGI
Sbjct: 241 REKIVSGTSRGQKCSYRHGISISDQRPGRNVFHKQNVLSSHGTRSWTSGCGGARHS-QGI 300

Query: 301 SGDRSLQDSL-MTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYSQSLLDIGQ 360
           S + S+ DSL MTSW P++++S SS+ SSSLQ  SESHSRH MTYPE E YS+SL     
Sbjct: 301 SDNLSVHDSLTMTSWTPRTSVSSSSNASSSLQLSSESHSRHRMTYPEPEGYSRSLH---- 360

Query: 361 TTAAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSLN 420
             AAE GIS+TSTN+NSFRCRN++GIAEVLLALERIE+EE  TYE AILLETS FL+ LN
Sbjct: 361 --AAEAGISNTSTNVNSFRCRNVNGIAEVLLALERIEREER-TYE-AILLETSTFLNRLN 420

Query: 421 IHDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTSS 480
           I+DQHRDMRLDIDNMTYE+LLDLEE MGTVSTALSE+ LTECL+RSI+QSKP+GG  T S
Sbjct: 421 IYDQHRDMRLDIDNMTYEQLLDLEEGMGTVSTALSEKELTECLHRSIYQSKPEGGTATGS 480

Query: 481 VEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSAHQLHPFK 538
           VEDLSDVKCCICQEEY SGDEVGRL CEHKYHV CIQQWLR+KNWCPICK++A  L PF+
Sbjct: 481 VEDLSDVKCCICQEEYASGDEVGRLQCEHKYHVACIQQWLRMKNWCPICKSTA--LSPFQ 526

BLAST of MC11g0521.1 vs. TAIR 10
Match: AT5G24870.1 (RING/U-box superfamily protein )

HSP 1 Score: 304.7 bits (779), Expect = 1.5e-82
Identity = 229/549 (41.71%), Postives = 319/549 (58.11%), Query Frame = 0

Query: 1   MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSN---HGSRLGCTGRICSSSGAEVGCSS 60
           MD +  +R    I+ RK  G+VL +NMK +D  +     SR+GC+ R+ S+ G  +   +
Sbjct: 1   MDGFKGKRTSRPIMPRKASGLVLHENMKKKDDKSVVPICSRIGCSSRVSSTKGDLIDHKA 60

Query: 61  KASSKRPFRSS-SGKEIVGSSSRSSSIRNFRKSFPDPFGK--LSSKLETDSSEDGSIQDE 120
           KA+    FRS  SGKE VGSSSRS S     K      G+  LSS L+ DSSE  S+ ++
Sbjct: 61  KATVS-SFRSPLSGKETVGSSSRSMSGFGGTKKASKVIGRRQLSSLLDMDSSESSSVNED 120

Query: 121 LEELEFISPPGMF-HTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQD 180
               E   P G    + + V SESSV  + ++ E GSSS  +     R+  QR  + ++D
Sbjct: 121 SPTSERSLPRGKTKESTISVHSESSVSGE-VVTEAGSSSRGTG----RSIHQRPDLVSRD 180

Query: 181 TSTSMRSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDG 240
              S   ++   ++N    +   R+L   S S+++P +S+       R+ N+ +K   DG
Sbjct: 181 ARVSNSEQNARASVN----KNGLRDLRNKSGSDVLPSNSTP-----TRKSNIFRKKTSDG 240

Query: 241 ENSSSSRGKKVSGTSCGQ---NCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACD 300
           E+SSSSRG K  G+  G    +     GI++S+ R+ RN       L S+   S+ S+  
Sbjct: 241 ESSSSSRGNKTEGSVVGGKNISSPQGNGITMSEPRRNRN-------LPSVRDNSVVSSST 300

Query: 301 GASHSYQGISGDRSLQDSLMTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYS 360
             S  Y G +G      +L     P       S ++ + +S   S+SR   +        
Sbjct: 301 RRSTGYYGRTGRAGAVATLQAPRPPTRADLNPSRSAEASRSPLNSYSRPISS----NGRL 360

Query: 361 QSLLDIGQTTAAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLET 420
           +SL+  G  + +E G+S +  N ++FR  N++G+AEVLLALERIEQ+EELTYEQ  +LET
Sbjct: 361 RSLMMPG--SPSEAGLSRSLMNRDTFRRYNMNGVAEVLLALERIEQDEELTYEQLAVLET 420

Query: 421 SLFLSSL-NIHDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSK 480
           +LFL+ + + HDQHRDMRLDIDNM+YEELL LEE+MGTVSTALSEEAL + L  SI++  
Sbjct: 421 NLFLNGMSSFHDQHRDMRLDIDNMSYEELLALEEKMGTVSTALSEEALLKSLKSSIYRPN 480

Query: 481 PQGGELTSSVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKT 538
            +  ++  + +D  DVKC ICQEEYV GDEVG L C+HKYHV C QQWLR+KNWCPICKT
Sbjct: 481 DESDDICLNKDD--DVKCSICQEEYVDGDEVGTLPCQHKYHVSCAQQWLRMKNWCPICKT 519

BLAST of MC11g0521.1 vs. TAIR 10
Match: AT5G24870.2 (RING/U-box superfamily protein )

HSP 1 Score: 304.7 bits (779), Expect = 1.5e-82
Identity = 229/549 (41.71%), Postives = 319/549 (58.11%), Query Frame = 0

Query: 1   MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSN---HGSRLGCTGRICSSSGAEVGCSS 60
           MD +  +R    I+ RK  G+VL +NMK +D  +     SR+GC+ R+ S+ G  +   +
Sbjct: 1   MDGFKGKRTSRPIMPRKASGLVLHENMKKKDDKSVVPICSRIGCSSRVSSTKGDLIDHKA 60

Query: 61  KASSKRPFRSS-SGKEIVGSSSRSSSIRNFRKSFPDPFGK--LSSKLETDSSEDGSIQDE 120
           KA+    FRS  SGKE VGSSSRS S     K      G+  LSS L+ DSSE  S+ ++
Sbjct: 61  KATVS-SFRSPLSGKETVGSSSRSMSGFGGTKKASKVIGRRQLSSLLDMDSSESSSVNED 120

Query: 121 LEELEFISPPGMF-HTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQD 180
               E   P G    + + V SESSV  + ++ E GSSS  +     R+  QR  + ++D
Sbjct: 121 SPTSERSLPRGKTKESTISVHSESSVSGE-VVTEAGSSSRGTG----RSIHQRPDLVSRD 180

Query: 181 TSTSMRSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDG 240
              S   ++   ++N    +   R+L   S S+++P +S+       R+ N+ +K   DG
Sbjct: 181 ARVSNSEQNARASVN----KNGLRDLRNKSGSDVLPSNSTP-----TRKSNIFRKKTSDG 240

Query: 241 ENSSSSRGKKVSGTSCGQ---NCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACD 300
           E+SSSSRG K  G+  G    +     GI++S+ R+ RN       L S+   S+ S+  
Sbjct: 241 ESSSSSRGNKTEGSVVGGKNISSPQGNGITMSEPRRNRN-------LPSVRDNSVVSSST 300

Query: 301 GASHSYQGISGDRSLQDSLMTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYS 360
             S  Y G +G      +L     P       S ++ + +S   S+SR   +        
Sbjct: 301 RRSTGYYGRTGRAGAVATLQAPRPPTRADLNPSRSAEASRSPLNSYSRPISS----NGRL 360

Query: 361 QSLLDIGQTTAAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLET 420
           +SL+  G  + +E G+S +  N ++FR  N++G+AEVLLALERIEQ+EELTYEQ  +LET
Sbjct: 361 RSLMMPG--SPSEAGLSRSLMNRDTFRRYNMNGVAEVLLALERIEQDEELTYEQLAVLET 420

Query: 421 SLFLSSL-NIHDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSK 480
           +LFL+ + + HDQHRDMRLDIDNM+YEELL LEE+MGTVSTALSEEAL + L  SI++  
Sbjct: 421 NLFLNGMSSFHDQHRDMRLDIDNMSYEELLALEEKMGTVSTALSEEALLKSLKSSIYRPN 480

Query: 481 PQGGELTSSVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKT 538
            +  ++  + +D  DVKC ICQEEYV GDEVG L C+HKYHV C QQWLR+KNWCPICKT
Sbjct: 481 DESDDICLNKDD--DVKCSICQEEYVDGDEVGTLPCQHKYHVSCAQQWLRMKNWCPICKT 519

BLAST of MC11g0521.1 vs. TAIR 10
Match: AT5G10650.1 (RING/U-box superfamily protein )

HSP 1 Score: 264.2 bits (674), Expect = 2.2e-70
Identity = 212/546 (38.83%), Postives = 288/546 (52.75%), Query Frame = 0

Query: 13  IVSRKGPGVVLRDNMKNRDHSN--HGSRLGCTGRICSSSGAEVGC---SSKASSKRPFRS 72
           +V RK  G+ LR+NM   D  N    SR+GCT ++ S+  + +G    ++K        +
Sbjct: 14  VVPRKASGLTLRENMNKTDGKNVPFCSRVGCTAKVTSTKRSRIGSTDNNTKVGLPPVPST 73

Query: 73  SSGKEIVGSSSRSSSIRNFRKSFPDPFGKL------------SSKLETDSSEDGSIQDEL 132
            + KEIVGSSSR+          P  FG L            SS L+T+SSE   I D+ 
Sbjct: 74  LNRKEIVGSSSRT----------PGGFGYLRKPAKVTARRQPSSSLDTESSETSCIHDDP 133

Query: 133 EELEFISPPGMFHTV-LHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDT 192
              E   P      V ++V  +S+V  + ++ + GSSS  ++  S   S           
Sbjct: 134 AATEPTLPRQKTKRVTINVHPQSAVSREVVITKAGSSSRGTSRISHPKSELGTRDALTGP 193

Query: 193 STSMRSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGE 252
           S S  S +    + GG SR+  RNL CNSVS+++P +S+S       + +VTKK   DGE
Sbjct: 194 SVSTSSGNSEHTVRGGLSRHRLRNLSCNSVSDVLPTNSNS-----ATKISVTKKKNADGE 253

Query: 253 NSSSSRGKKVS---GTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDG 312
           +S SS+G K S        Q   +  G+++SD R+ R       V+ S+   S   +   
Sbjct: 254 SSLSSKGSKTSVLVPKVRNQISSHGNGVTVSDNRRNR-------VVPSIRDSSTVVSNGC 313

Query: 313 ASHSYQGISGDRSLQDSLMTS-WMPQSNISGSSHTSSSL--QSRSESHSRHHMTYPEHED 372
               Y G S       S  TS  MP        + S S    +   S  R H   P    
Sbjct: 314 RRAGYFGRSERLGATASSATSRQMPHPTTPTDPNPSLSFCPSNIYSSTGRVHSNMP---- 373

Query: 373 YSQSLLDIGQTTAAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILL 432
                   G  T A+   SS+  N +     N++GIAEVLLALERIE +EELTYEQ   +
Sbjct: 374 --------GSPTEADP--SSSLVNRDGLSHYNMNGIAEVLLALERIEHDEELTYEQLASI 433

Query: 433 ETSLFLSSL-NIHDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQ 492
           ET+LF S +   +DQHRDMRLDIDNM+YEELL L ++MGTVSTALSEEAL+  L +SI+Q
Sbjct: 434 ETNLFSSGMFRFYDQHRDMRLDIDNMSYEELLALGDKMGTVSTALSEEALSRSLKQSIYQ 493

Query: 493 SKPQGGELTSSVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPIC 534
              + G ++   +D  D+KC ICQEEYV GDE+G + C+H YHV C+QQWLR+KNWCPIC
Sbjct: 494 ETDETGSISLYKDD--DIKCSICQEEYVDGDELGTIPCQHMYHVSCVQQWLRMKNWCPIC 521

BLAST of MC11g0521.1 vs. TAIR 10
Match: AT5G10650.2 (RING/U-box superfamily protein )

HSP 1 Score: 264.2 bits (674), Expect = 2.2e-70
Identity = 212/546 (38.83%), Postives = 288/546 (52.75%), Query Frame = 0

Query: 13  IVSRKGPGVVLRDNMKNRDHSN--HGSRLGCTGRICSSSGAEVGC---SSKASSKRPFRS 72
           +V RK  G+ LR+NM   D  N    SR+GCT ++ S+  + +G    ++K        +
Sbjct: 14  VVPRKASGLTLRENMNKTDGKNVPFCSRVGCTAKVTSTKRSRIGSTDNNTKVGLPPVPST 73

Query: 73  SSGKEIVGSSSRSSSIRNFRKSFPDPFGKL------------SSKLETDSSEDGSIQDEL 132
            + KEIVGSSSR+          P  FG L            SS L+T+SSE   I D+ 
Sbjct: 74  LNRKEIVGSSSRT----------PGGFGYLRKPAKVTARRQPSSSLDTESSETSCIHDDP 133

Query: 133 EELEFISPPGMFHTV-LHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDT 192
              E   P      V ++V  +S+V  + ++ + GSSS  ++  S   S           
Sbjct: 134 AATEPTLPRQKTKRVTINVHPQSAVSREVVITKAGSSSRGTSRISHPKSELGTRDALTGP 193

Query: 193 STSMRSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGE 252
           S S  S +    + GG SR+  RNL CNSVS+++P +S+S       + +VTKK   DGE
Sbjct: 194 SVSTSSGNSEHTVRGGLSRHRLRNLSCNSVSDVLPTNSNS-----ATKISVTKKKNADGE 253

Query: 253 NSSSSRGKKVS---GTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDG 312
           +S SS+G K S        Q   +  G+++SD R+ R       V+ S+   S   +   
Sbjct: 254 SSLSSKGSKTSVLVPKVRNQISSHGNGVTVSDNRRNR-------VVPSIRDSSTVVSNGC 313

Query: 313 ASHSYQGISGDRSLQDSLMTS-WMPQSNISGSSHTSSSL--QSRSESHSRHHMTYPEHED 372
               Y G S       S  TS  MP        + S S    +   S  R H   P    
Sbjct: 314 RRAGYFGRSERLGATASSATSRQMPHPTTPTDPNPSLSFCPSNIYSSTGRVHSNMP---- 373

Query: 373 YSQSLLDIGQTTAAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILL 432
                   G  T A+   SS+  N +     N++GIAEVLLALERIE +EELTYEQ   +
Sbjct: 374 --------GSPTEADP--SSSLVNRDGLSHYNMNGIAEVLLALERIEHDEELTYEQLASI 433

Query: 433 ETSLFLSSL-NIHDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQ 492
           ET+LF S +   +DQHRDMRLDIDNM+YEELL L ++MGTVSTALSEEAL+  L +SI+Q
Sbjct: 434 ETNLFSSGMFRFYDQHRDMRLDIDNMSYEELLALGDKMGTVSTALSEEALSRSLKQSIYQ 493

Query: 493 SKPQGGELTSSVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPIC 534
              + G ++   +D  D+KC ICQEEYV GDE+G + C+H YHV C+QQWLR+KNWCPIC
Sbjct: 494 ETDETGSISLYKDD--DIKCSICQEEYVDGDELGTIPCQHMYHVSCVQQWLRMKNWCPIC 521

BLAST of MC11g0521.1 vs. TAIR 10
Match: AT4G31450.1 (RING/U-box superfamily protein )

HSP 1 Score: 238.4 bits (607), Expect = 1.3e-62
Identity = 207/554 (37.36%), Postives = 296/554 (53.43%), Query Frame = 0

Query: 1   MDEYSSRR-VHVGIVSRKGPGVVLRDNMKNRDHSNHGSRLGCTGRICSSSGAE------V 60
           MD +SS+R +     S+K  GVV RD    R +SN      C   ICS           V
Sbjct: 1   MDRWSSKRAMEARPDSKKKGGVVFRD----RFNSN-----SCKVPICSDEKKSMNFTRFV 60

Query: 61  GCSSK--ASSKRPFRSS-SGKEIVGSSSR-----SSSIRNFRKSFPDPFGKLSSKLETDS 120
           G S K   S    +RSS +GKE++G+SS+     SSS+++  K    PF +++  +++  
Sbjct: 61  GSSDKKEKSVLSTYRSSPNGKEVIGTSSKICISSSSSVKSGEK---QPFSQIA--IDSSE 120

Query: 121 SEDGSIQDELEELEFISPPGMFHTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQ 180
           S  GS +DE+E      P G     ++ K    +    I  E   S   S+++ +R   Q
Sbjct: 121 SSRGS-EDEVESEILQVPLGRDKRRMNNK---LIYGKVITPEAECSKLPSSSRIKRGFRQ 180

Query: 181 RYGVDNQDTSTSMRSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNV 240
           R+G+  Q+               G + +  S N  C+ +   + P     ++ L R+ + 
Sbjct: 181 RFGLSKQEFHP------------GPSGQSTSANRGCSPLLSGVIPSGFGLDKRLSRKADT 240

Query: 241 TKKMIYDGENSSSS--RGKKVS---GTSCGQNCGYRRGISISDQRQGRN-MFHRQNVLSS 300
             K    GE+SSSS  RGK V+        +     RG S+SD R+ R+ +    N ++S
Sbjct: 241 ISKTKVYGESSSSSSARGKNVTEPPPVEVRRRSFNPRG-SVSDSRRARHCILDDDNDVAS 300

Query: 301 LGTRSLASACDGASHSYQGISGDRSLQDSLMTSWMPQSNISGSSHTSSSLQSRSESHSRH 360
           +G++ LA+     ++S    SG   L  S+  + M Q+  S + ++  SL+  S      
Sbjct: 301 VGSQRLAN----RNNSRIRGSGRDGL-SSVTAAEMSQTETSNNLNSPVSLELFS------ 360

Query: 361 HMTYPEHEDYSQSLLDIGQTTAAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEE 420
              +PE                   G+S +  + +SFR  NLDGI+E+L  L+RIEQ+ E
Sbjct: 361 --GFPEF------------------GLSGSLLSHDSFRSYNLDGISEILPELDRIEQDIE 420

Query: 421 LTYEQAILLETSLFLSSLNIHDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTE 480
           L YE  +++ET L L  L+ HDQHRDMRLDIDNM+YEELL LEER+GTVSTAL+EEA+++
Sbjct: 421 LNYEDLLIMETGLLLGGLSFHDQHRDMRLDIDNMSYEELLALEERIGTVSTALTEEAISK 480

Query: 481 CLNRSIFQSKPQG-GELTSSVED-LSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQW 532
           CL  SI+Q KP   G +T S  D   D KC ICQEEY  GDEVGRLHCEH YHVKC+Q+W
Sbjct: 481 CLKTSIYQMKPLSYGSITKSPSDNKEDAKCSICQEEYTIGDEVGRLHCEHTYHVKCVQEW 492