MC11g0269.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC11g0269.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	3-oxo-Delta(4,5)-steroid 5-beta-reductase
Location	MC11: 2147984 .. 2149932 (+)
Sequence length	1848
RNA-Seq Expression	MC11g0269.1
Synteny	MC11g0269.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTAAATTATGCACAATCAATAAAATATCTTTTTCAGAATTTTCTACATTTTGAATAAAAAACAAATAAATTGATTATTTTTTTTAAATTAGAAATACTTTTCTATTGTTAAAAAACAATTTCGAAAACGTTGTAAAAAGAAAATCCTGGGCGGGCAGTGTGGCGGTTCGGGTTCAATGATTGTGCTTCCACGTTTCACCGCTCGCGTCACGGTTTGCGTTTCGTTTTGCAATCGAAAAGTCTGATCTGTAAAATCCGTTCGGTTATAAGCTTTTGAACCACTGATCGAATTCCTCAATTCAGTATTGGTTTCTTATCTTCTCCCTCTTCCCAAATTTCTCAACCTACTTCCAATTCCCCGACGGAAGATGAGCTGGTGGTGGGCCGGAGCAATTGGCGCTGCTAAGGTAATACTGATGATCCCAATTTTTTGCATCCGCGGCCGTGTTTTTGTCTCCGAAACGTCTTCCTGTTTCTCTAATCGATTTCTTTAATCCCATTTTTGCAGAAGAAATTCGAAGAAGATGAACCTCCGAGGAGCTTTGAGAGCGTCGCTTTGGTGATCGGTGTTACTGGTATCGTCGGGAATAGCCTCGCCGAGATTCTTCCTCTCGCCGACACGCCCGGCGGCCCCTGGAAGGTCTACGGCGTCGCTCGGAGGCCCAGGCCAAACTGGAACGCCGACCATCCCATCGAGTACATCCAATGCGACATCTCCGATCCGCAAGACGCAGAAGCCAAACTCTCGCAGCTCGCTGATGTGACTCATGTCTTCTATGTCACCTGGACCAATCGATCCACCGAGATCGAGAACTGCGAGGCCAACGGAAAAATGCTCCGCAATGTTTTGCGATCAGTGATTCCGAACGCAACGAATCTCCGCCATATCTCCCTTCAAACCGGTGCGAAGCACTACATTGGATCGTTCGAGAGCATCTCCGGTAAATCGACGCAGCAGCACGATCCCCCTTTCACAGAGGATTTGCCTCGATTGGAATCCCCAAATTTCTATTACGCACTAGAAGATATACTGTTCGAAGAGGTAGAGGAGAGGAAGAATCTGACATGGGCGGTGATCCGACCCAACACTATCTTCGGATTTTCCCCTTTCAGCTTGATGAACATCGTCGGAACCCTATGCGTCTACGCCGCCATATGCAAGCACGAGGGACGCCCGTTGAAATTCCCCGGCAACAGAGCGGATTGGGAGGGTTTCAGGGTGGCGTCCGACGCAGATCTGATAGCGGAGCAGCATATATGGACGGCGGTGGATCCGTACGCGAAGAACGAGGCGTTCAACTGCAACAACGGCGACGTTTTCAAGTGGAAGCATTTCTGGAAGGTGCTGGCAGAGCAGTTTAACATCGAGGATTATGGGTTCGAAGAAGAGGATGAGAGATTGTCGTTGGAGAAGACGATGAAGGAGAAAGGTTCAGTGTGGGAAGAAATTGTTAAGGAGAATCAGCTGCAGCAAACCAAAGTGGAGGAGGTGGGTACTTGGTGGTTTGCAGATTTTACTTTGAGCGGCTCAGGCATGATAGATAGTATGAACAAGAGCAAGGAGCATGGGTTCTTGGGATTCAGGAACTCCAAGAATTCATTCATTTCTTGGATTGACAAGTTTAAAGCTTACAAGATCGTGCCATGAACATGATCGGCGACTATTCTTTCTCAGCGTTCAAAATGTGAAATCTTCTTCTTTGATTTAATCCTTCCATCTGTTTGCTCTTCATTGGTGCTTCCCTGTTACTCCAACCTCTTTCGTTGGTTTCTCCATTGCTTTTGCAATAAGATTGAGCATAGAAAATGTTGGTTTAATTACACTTCTACTACTAGAGTAGCTTTAATTTTGTATTTAGTAGCTTTAATTTTACCTGTTTCAGTATTGAACCCACCTCACTCGTGACTCGAGAGGAGCAACAGAAATTTAACACTAACAATTAACAAAC

mRNA sequence

CTTAAATTATGCACAATCAATAAAATATCTTTTTCAGAATTTTCTACATTTTGAATAAAAAACAAATAAATTGATTATTTTTTTTAAATTAGAAATACTTTTCTATTGTTAAAAAACAATTTCGAAAACGTTGTAAAAAGAAAATCCTGGGCGGGCAGTGTGGCGGTTCGGGTTCAATGATTGTGCTTCCACGTTTCACCGCTCGCGTCACGGTTTGCGTTTCGTTTTGCAATCGAAAAGTCTGATCTGTAAAATCCGTTCGGTTATAAGCTTTTGAACCACTGATCGAATTCCTCAATTCAGTATTGGTTTCTTATCTTCTCCCTCTTCCCAAATTTCTCAACCTACTTCCAATTCCCCGACGGAAGATGAGCTGGTGGTGGGCCGGAGCAATTGGCGCTGCTAAGAAGAAATTCGAAGAAGATGAACCTCCGAGGAGCTTTGAGAGCGTCGCTTTGGTGATCGGTGTTACTGGTATCGTCGGGAATAGCCTCGCCGAGATTCTTCCTCTCGCCGACACGCCCGGCGGCCCCTGGAAGGTCTACGGCGTCGCTCGGAGGCCCAGGCCAAACTGGAACGCCGACCATCCCATCGAGTACATCCAATGCGACATCTCCGATCCGCAAGACGCAGAAGCCAAACTCTCGCAGCTCGCTGATGTGACTCATGTCTTCTATGTCACCTGGACCAATCGATCCACCGAGATCGAGAACTGCGAGGCCAACGGAAAAATGCTCCGCAATGTTTTGCGATCAGTGATTCCGAACGCAACGAATCTCCGCCATATCTCCCTTCAAACCGGTGCGAAGCACTACATTGGATCGTTCGAGAGCATCTCCGGTAAATCGACGCAGCAGCACGATCCCCCTTTCACAGAGGATTTGCCTCGATTGGAATCCCCAAATTTCTATTACGCACTAGAAGATATACTGTTCGAAGAGGTAGAGGAGAGGAAGAATCTGACATGGGCGGTGATCCGACCCAACACTATCTTCGGATTTTCCCCTTTCAGCTTGATGAACATCGTCGGAACCCTATGCGTCTACGCCGCCATATGCAAGCACGAGGGACGCCCGTTGAAATTCCCCGGCAACAGAGCGGATTGGGAGGGTTTCAGGGTGGCGTCCGACGCAGATCTGATAGCGGAGCAGCATATATGGACGGCGGTGGATCCGTACGCGAAGAACGAGGCGTTCAACTGCAACAACGGCGACGTTTTCAAGTGGAAGCATTTCTGGAAGGTGCTGGCAGAGCAGTTTAACATCGAGGATTATGGGTTCGAAGAAGAGGATGAGAGATTGTCGTTGGAGAAGACGATGAAGGAGAAAGGTTCAGTGTGGGAAGAAATTGTTAAGGAGAATCAGCTGCAGCAAACCAAAGTGGAGGAGGTGGGTACTTGGTGGTTTGCAGATTTTACTTTGAGCGGCTCAGGCATGATAGATAGTATGAACAAGAGCAAGGAGCATGGGTTCTTGGGATTCAGGAACTCCAAGAATTCATTCATTTCTTGGATTGACAAGTTTAAAGCTTACAAGATCGTGCCATGAACATGATCGGCGACTATTCTTTCTCAGCGTTCAAAATGTGAAATCTTCTTCTTTGATTTAATCCTTCCATCTGTTTGCTCTTCATTGGTGCTTCCCTGTTACTCCAACCTCTTTCGTTGGTTTCTCCATTGCTTTTGCAATAAGATTGAGCATAGAAAATGTTGGTTTAATTACACTTCTACTACTAGAGTAGCTTTAATTTTGTATTTAGTAGCTTTAATTTTACCTGTTTCAGTATTGAACCCACCTCACTCGTGACTCGAGAGGAGCAACAGAAATTTAACACTAACAATTAACAAAC

Coding sequence (CDS)

ATGAGCTGGTGGTGGGCCGGAGCAATTGGCGCTGCTAAGAAGAAATTCGAAGAAGATGAACCTCCGAGGAGCTTTGAGAGCGTCGCTTTGGTGATCGGTGTTACTGGTATCGTCGGGAATAGCCTCGCCGAGATTCTTCCTCTCGCCGACACGCCCGGCGGCCCCTGGAAGGTCTACGGCGTCGCTCGGAGGCCCAGGCCAAACTGGAACGCCGACCATCCCATCGAGTACATCCAATGCGACATCTCCGATCCGCAAGACGCAGAAGCCAAACTCTCGCAGCTCGCTGATGTGACTCATGTCTTCTATGTCACCTGGACCAATCGATCCACCGAGATCGAGAACTGCGAGGCCAACGGAAAAATGCTCCGCAATGTTTTGCGATCAGTGATTCCGAACGCAACGAATCTCCGCCATATCTCCCTTCAAACCGGTGCGAAGCACTACATTGGATCGTTCGAGAGCATCTCCGGTAAATCGACGCAGCAGCACGATCCCCCTTTCACAGAGGATTTGCCTCGATTGGAATCCCCAAATTTCTATTACGCACTAGAAGATATACTGTTCGAAGAGGTAGAGGAGAGGAAGAATCTGACATGGGCGGTGATCCGACCCAACACTATCTTCGGATTTTCCCCTTTCAGCTTGATGAACATCGTCGGAACCCTATGCGTCTACGCCGCCATATGCAAGCACGAGGGACGCCCGTTGAAATTCCCCGGCAACAGAGCGGATTGGGAGGGTTTCAGGGTGGCGTCCGACGCAGATCTGATAGCGGAGCAGCATATATGGACGGCGGTGGATCCGTACGCGAAGAACGAGGCGTTCAACTGCAACAACGGCGACGTTTTCAAGTGGAAGCATTTCTGGAAGGTGCTGGCAGAGCAGTTTAACATCGAGGATTATGGGTTCGAAGAAGAGGATGAGAGATTGTCGTTGGAGAAGACGATGAAGGAGAAAGGTTCAGTGTGGGAAGAAATTGTTAAGGAGAATCAGCTGCAGCAAACCAAAGTGGAGGAGGTGGGTACTTGGTGGTTTGCAGATTTTACTTTGAGCGGCTCAGGCATGATAGATAGTATGAACAAGAGCAAGGAGCATGGGTTCTTGGGATTCAGGAACTCCAAGAATTCATTCATTTCTTGGATTGACAAGTTTAAAGCTTACAAGATCGTGCCATGA

Protein sequence

MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYGVARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANGKMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNFYYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFPGNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIEDYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSMNKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP

Homology

[Close]

BLAST of MC11g0269.1 vs. ExPASy Swiss-Prot
Match: Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 2.6e-182
Identity = 294/392 (75.00%), Postives = 334/392 (85.20%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDEP +SFESVAL+IGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
           VARRPRP WNADHPI+YIQCD+SD +D  +KLS L DVTHVFYVTWTNR +E ENCEANG
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
            MLRNVL+++IP A NLRH+ LQTG KHY+G F ++ G    +HDPPFTED+PRL+  NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNVDG---PRHDPPFTEDMPRLQIQNF 180

Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
           YY  EDILFEE+++ + +TW++ RPN IFGFSP+SLMNIVGTLCVYAAICKHEG PL FP
Sbjct: 181 YYTQEDILFEEIKKIETVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFP 240

Query: 241 GNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
           G++  WEGF  ASDADLIAEQ IW AVDPYAKNEAFNCNN D+FKWKH WK+LAEQF IE
Sbjct: 241 GSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIE 300

Query: 301 DYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
           +YGF EE + L L + MK K  VWEE+VKENQLQ+ K+EEVG WWFAD  L   GMIDSM
Sbjct: 301 EYGF-EEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDSM 360

Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
           NKSKE+GFLGFRNS NSFISWIDK+KA+KIVP
Sbjct: 361 NKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]

BLAST of MC11g0269.1 vs. ExPASy Swiss-Prot
Match: A0A221J5W8 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 2.9e-173
Identity = 279/392 (71.17%), Postives = 325/392 (82.91%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDE PR++ESVAL++GVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKIDEDEAPRNYESVALIVGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
           VARRPRP+WN DHPI YI CD+ D  D EAKLS L DVTH+FY TWT RSTE ENCEANG
Sbjct: 61  VARRPRPSWNEDHPITYISCDVLDSVDVEAKLSPLTDVTHIFYATWTKRSTEKENCEANG 120

Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
           KML+NVL ++IPN  NL+HI LQTG KHY+G+FE +  K    HDPP TEDLPRL+S N+
Sbjct: 121 KMLKNVLNAMIPNCPNLKHICLQTGRKHYLGAFEDLKSKC---HDPPLTEDLPRLDSQNY 180

Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
           YY  EDILFEEV++++ LTW+V RP TIFGFSP+S+MN+VGTLCVYAAICKHEG  L+FP
Sbjct: 181 YYTQEDILFEEVQKKEGLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLRFP 240

Query: 241 GNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
           G +  W+G+   SDADLIAE  IW AVDPYAKNEAFN +NGDVFKWKHFWKVLAEQF +E
Sbjct: 241 GCKGAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVE 300

Query: 301 DYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
             G  EE +++ L+  MK+KG VW++IV+EN L  TK+E+VG WWF+D  L     +D+M
Sbjct: 301 -CGEYEEGQQVKLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLGNECWLDTM 360

Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
           NKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 NKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 388

[Close]

BLAST of MC11g0269.1 vs. ExPASy Swiss-Prot
Match: A0A221J5X3 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 2.7e-171
Identity = 278/392 (70.92%), Postives = 324/392 (82.65%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
           MSWWWAGAIGAAKK+ +EDE PR++ESVAL++GVTGIVGNSLAEILPL+DTP GPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKRIDEDEAPRNYESVALIVGVTGIVGNSLAEILPLSDTPCGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
           VARRPRP+WN DHPI YI CD+ D  D EAKLS L DVTH+FY TWT RSTE ENCEANG
Sbjct: 61  VARRPRPSWNEDHPITYISCDVLDSVDVEAKLSPLTDVTHIFYATWTKRSTEKENCEANG 120

Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
           KML+NVL ++IPN  NL+HI LQTG KHY+G+FE    K   +HDPP TEDLPRL+S NF
Sbjct: 121 KMLKNVLNAMIPNCPNLKHICLQTGRKHYLGAFEDWKIK---RHDPPLTEDLPRLDSQNF 180

Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
           YY  EDILFEEV+++++LTW+V RP TIFGFSP+S+MN+VGTLCVYAAICKHEG  L+FP
Sbjct: 181 YYTQEDILFEEVQKKESLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLRFP 240

Query: 241 GNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
           G +  W+G+   SDADLIAE  IW AVDPYAKNEAFN +NGDVFKWKHFWKVLAEQF +E
Sbjct: 241 GCKGAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVE 300

Query: 301 DYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
             G  EE + + L+  MK+KG VW++IV+EN L  TK+E+VG WWF+D  L     +D+M
Sbjct: 301 -CGEYEEGKEVKLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLGNECWLDTM 360

Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
           NKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 NKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 388

[Close]

BLAST of MC11g0269.1 vs. ExPASy Swiss-Prot
Match: A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 1.5e-169
Identity = 277/392 (70.66%), Postives = 316/392 (80.61%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
           MSWWWAGAIGAA+KKFEED+ PR  +SVAL+IGVTGIVGNSLAEILP++DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAARKKFEEDDAPRDCQSVALIIGVTGIVGNSLAEILPISDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
           VARRPRP WNADHP+EYIQCDISD  D   KLS L DVTH+F+VTW NR TE E CE NG
Sbjct: 61  VARRPRPAWNADHPVEYIQCDISDASDTHTKLSPLTDVTHIFWVTWANRPTESECCELNG 120

Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
            MLRNVL ++IP A NL HI LQTG KHYIG FE+      + H+PPFTED+PRL +PNF
Sbjct: 121 TMLRNVLNALIPKAANLHHICLQTGHKHYIGPFEAFG--KIKPHEPPFTEDMPRLNAPNF 180

Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
           YY LED+L E  E++  L W+V RP  IFGFSPFS+MNI+GTLCVYAAICKHE  PLKFP
Sbjct: 181 YYTLEDMLVEASEKKAGLNWSVHRPAVIFGFSPFSMMNIIGTLCVYAAICKHENTPLKFP 240

Query: 241 GNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
           G +A W  + VASDADLIAE  IW AVDPYAKNEAFNC+NGD+FKWKH WKVLAEQF +E
Sbjct: 241 GTKAAWNCYSVASDADLIAEHQIWAAVDPYAKNEAFNCSNGDLFKWKHLWKVLAEQFGVE 300

Query: 301 DYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
              F+E ++  SL + MK+KG VWEEIV+EN L  TK+E V TWWFAD  L G  ++DSM
Sbjct: 301 YAEFDESEKPTSLVERMKDKGPVWEEIVRENGLHTTKLEGVATWWFADVILGGECLLDSM 360

Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
           NKSKEHG+LGFRN+KNS IS IDK KA+KIVP
Sbjct: 361 NKSKEHGYLGFRNTKNSLISVIDKMKAHKIVP 390

[Close]

BLAST of MC11g0269.1 vs. ExPASy Swiss-Prot
Match: Q6PQJ9 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 1.4e-167
Identity = 278/392 (70.92%), Postives = 316/392 (80.61%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
           MSWWWAGAIGAAKK+ EED+      SVAL++GVTGI+GNSLAEILPLADTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKRLEEDDAQPKHSSVALIVGVTGIIGNSLAEILPLADTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
           VARR RP W+ D+PI Y+QCDISDP D++AKLS L DVTHVFYVTW NRSTE ENCEAN 
Sbjct: 61  VARRTRPAWHEDNPINYVQCDISDPDDSQAKLSPLTDVTHVFYVTWANRSTEQENCEANS 120

Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
           KM RNVL +VIPN  NL+HISLQTG KHY+G FES      + HDPP+TEDLPRL+  NF
Sbjct: 121 KMFRNVLDAVIPNCPNLKHISLQTGRKHYMGPFESYG--KIESHDPPYTEDLPRLKYMNF 180

Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
           YY LEDI+ EEVE+++ LTW+V RP  IFGFSP+S+MN+VGTLCVYAAICKHEG+ L+F 
Sbjct: 181 YYDLEDIMLEEVEKKEGLTWSVHRPGNIFGFSPYSMMNLVGTLCVYAAICKHEGKVLRFT 240

Query: 241 GNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
           G +A W+G+   SDADLIAE HIW AVDPYAKNEAFN +NGDVFKWKHFWKVLAEQF + 
Sbjct: 241 GCKAAWDGYSDCSDADLIAEHHIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGV- 300

Query: 301 DYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
             G  EE   L L+  MK K  VWEEIV+EN L  TK+++VG WWF D  L     +DSM
Sbjct: 301 GCGEYEEGVDLKLQDLMKGKEPVWEEIVRENGLTPTKLKDVGIWWFGDVILGNECFLDSM 360

Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
           NKSKEHGFLGFRNSKN+FISWIDK KAYKIVP
Sbjct: 361 NKSKEHGFLGFRNSKNAFISWIDKAKAYKIVP 389

[Close]

BLAST of MC11g0269.1 vs. NCBI nr
Match: XP_022159803.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase [Momordica charantia])

HSP 1 Score: 822 bits (2122), Expect = 6.29e-301
Identity = 392/392 (100.00%), Postives = 392/392 (100.00%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
           VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG
Sbjct: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120

Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
           KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF
Sbjct: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180

Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
           YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP
Sbjct: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240

Query: 241 GNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
           GNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE
Sbjct: 241 GNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300

Query: 301 DYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
           DYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM
Sbjct: 301 DYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360

Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 392
           NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP
Sbjct: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 392

[Close]

BLAST of MC11g0269.1 vs. NCBI nr
Match: XP_004152001.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus] >KGN58303.1 hypothetical protein Csa_017526 [Cucumis sativus])

HSP 1 Score: 721 bits (1860), Expect = 5.85e-261
Identity = 338/394 (85.79%), Postives = 363/394 (92.13%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
           VARRPRP WNADHPIEYIQCD+SDPQDAE KLSQLADVTH+FYVTWTNR+TEIENCEAN 
Sbjct: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120

Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
           KMLRNVLRSVIPNA NLRHI LQTG KHY+GSFESI  KS+Q+HDPPFTEDLPRLE PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180

Query: 181 YYALEDILFEEVEE--RKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY  ED+L+EE+E+  +K+LTWAVIRPN IFGFSPFSLMN+VGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPGN+  WE F+VASDADLIAEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMID 360
           IE+YGF+EE E L+L   MK+K  VW+EIVKENQLQQTK+EE+GTWWF D   S SG ID
Sbjct: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 392
           SMNKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394

[Close]

BLAST of MC11g0269.1 vs. NCBI nr
Match: XP_022940360.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucurbita moschata] >KAG6608438.1 3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037773.1 3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 706 bits (1823), Expect = 2.45e-255
Identity = 334/394 (84.77%), Postives = 356/394 (90.36%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
           VARRPRPNWNADHPIEYIQCD+SDP+DAEAKLS+L DVTHVFYVTWTN  TEIENCE NG
Sbjct: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
           KMLRNVLRSVIPNA NLRHI LQTG KHYIGSFE+I    TQ+HDPPFTED+PRL+SPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180

Query: 181 YYALEDILFEEVE--ERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY  ED+LFEE+E  ++K+LTWAVIRPNTIFGFSPFSLMNIVGTLC YAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG ++ WEG+ V SDADLIAEQHIWTAVDPYAKNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMID 360
           IEDYGFEE  ERL L + MK K +VWEEIVKENQLQ TK+E+V  WW  D  LS SG ID
Sbjct: 301 IEDYGFEE-GERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 392
           SMNKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]

BLAST of MC11g0269.1 vs. NCBI nr
Match: XP_008447374.1 (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo] >KAA0037954.1 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo var. makuwa])

HSP 1 Score: 704 bits (1817), Expect = 2.01e-254
Identity = 330/394 (83.76%), Postives = 358/394 (90.86%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
           VARRPRP WNADHPIEYIQCD+SDPQDAE KLSQLADVTH+FYVTWTNR+ EI+NCEAN 
Sbjct: 61  VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120

Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
           +MLRNVLRSVIPNA NLRHI LQTG KHY+GSFES+  KS+Q+H+PPFTEDLPRLE PNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180

Query: 181 YYALEDILFEEVEE--RKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY  ED+LFEE+E+  +K+LTWAVIRPN IFGFSPFSLMNIVGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG +  WE F+ ASDADL+AEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMID 360
           IE+YGFEE  ERL+L   MK+K  VW+EIVKENQLQ TK+EE+G WW+ D   S SG ID
Sbjct: 301 IEEYGFEE-GERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 392
           SMNKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]

BLAST of MC11g0269.1 vs. NCBI nr
Match: XP_038899926.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase [Benincasa hispida])

HSP 1 Score: 703 bits (1814), Expect = 5.75e-254
Identity = 332/394 (84.26%), Postives = 357/394 (90.61%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
           +ARRPRP WNADHPIEYIQCDISDP+DAEAKLSQL DVTH+FYVTWTNR TEIENCEAN 
Sbjct: 61  IARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANV 120

Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
           KMLRNVLRSVIPNA NLRHI LQTG KHY+GS +SI  KS Q HDPPFTEDLPRLE PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNF 180

Query: 181 YYALEDILFEEVEE--RKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY+ ED+LFEE+E+  +K+LTWAVIRPN IFGFSPFSL+NIVGTLCVYAAICKHEGR LK
Sbjct: 181 YYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLK 240

Query: 241 FPGNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG+++ WE F+V SDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMID 360
           IEDYGFE+  ERL+L + MK+K  VW+EIVKENQLQ TK+EE+  WWF D   S SG ID
Sbjct: 301 IEDYGFED-GERLTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 392
           SMNKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]

BLAST of MC11g0269.1 vs. ExPASy TrEMBL
Match: A0A6J1DZW3 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Momordica charantia OX=3673 GN=LOC111026111 PE=3 SV=1)

HSP 1 Score: 822 bits (2122), Expect = 3.04e-301
Identity = 392/392 (100.00%), Postives = 392/392 (100.00%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
           VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG
Sbjct: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120

Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
           KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF
Sbjct: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180

Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
           YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP
Sbjct: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240

Query: 241 GNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
           GNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE
Sbjct: 241 GNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300

Query: 301 DYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
           DYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM
Sbjct: 301 DYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360

Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 392
           NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP
Sbjct: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 392

[Close]

BLAST of MC11g0269.1 vs. ExPASy TrEMBL
Match: A0A0A0L8Y0 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G610790 PE=3 SV=1)

HSP 1 Score: 721 bits (1860), Expect = 2.83e-261
Identity = 338/394 (85.79%), Postives = 363/394 (92.13%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
           VARRPRP WNADHPIEYIQCD+SDPQDAE KLSQLADVTH+FYVTWTNR+TEIENCEAN 
Sbjct: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120

Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
           KMLRNVLRSVIPNA NLRHI LQTG KHY+GSFESI  KS+Q+HDPPFTEDLPRLE PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180

Query: 181 YYALEDILFEEVEE--RKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY  ED+L+EE+E+  +K+LTWAVIRPN IFGFSPFSLMN+VGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPGN+  WE F+VASDADLIAEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMID 360
           IE+YGF+EE E L+L   MK+K  VW+EIVKENQLQQTK+EE+GTWWF D   S SG ID
Sbjct: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 392
           SMNKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394

[Close]

BLAST of MC11g0269.1 vs. ExPASy TrEMBL
Match: A0A6J1FQC1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucurbita moschata OX=3662 GN=LOC111445997 PE=3 SV=1)

HSP 1 Score: 706 bits (1823), Expect = 1.18e-255
Identity = 334/394 (84.77%), Postives = 356/394 (90.36%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
           VARRPRPNWNADHPIEYIQCD+SDP+DAEAKLS+L DVTHVFYVTWTN  TEIENCE NG
Sbjct: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
           KMLRNVLRSVIPNA NLRHI LQTG KHYIGSFE+I    TQ+HDPPFTED+PRL+SPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180

Query: 181 YYALEDILFEEVE--ERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY  ED+LFEE+E  ++K+LTWAVIRPNTIFGFSPFSLMNIVGTLC YAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG ++ WEG+ V SDADLIAEQHIWTAVDPYAKNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMID 360
           IEDYGFEE  ERL L + MK K +VWEEIVKENQLQ TK+E+V  WW  D  LS SG ID
Sbjct: 301 IEDYGFEE-GERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 392
           SMNKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]

BLAST of MC11g0269.1 vs. ExPASy TrEMBL
Match: A0A5A7T3B3 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G001350 PE=3 SV=1)

HSP 1 Score: 704 bits (1817), Expect = 9.72e-255
Identity = 330/394 (83.76%), Postives = 358/394 (90.86%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
           VARRPRP WNADHPIEYIQCD+SDPQDAE KLSQLADVTH+FYVTWTNR+ EI+NCEAN 
Sbjct: 61  VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120

Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
           +MLRNVLRSVIPNA NLRHI LQTG KHY+GSFES+  KS+Q+H+PPFTEDLPRLE PNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180

Query: 181 YYALEDILFEEVEE--RKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY  ED+LFEE+E+  +K+LTWAVIRPN IFGFSPFSLMNIVGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG +  WE F+ ASDADL+AEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMID 360
           IE+YGFEE  ERL+L   MK+K  VW+EIVKENQLQ TK+EE+G WW+ D   S SG ID
Sbjct: 301 IEEYGFEE-GERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 392
           SMNKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]

BLAST of MC11g0269.1 vs. ExPASy TrEMBL
Match: A0A1S3BHA7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC103489839 PE=3 SV=1)

HSP 1 Score: 704 bits (1817), Expect = 9.72e-255
Identity = 330/394 (83.76%), Postives = 358/394 (90.86%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
           VARRPRP WNADHPIEYIQCD+SDPQDAE KLSQLADVTH+FYVTWTNR+ EI+NCEAN 
Sbjct: 61  VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120

Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
           +MLRNVLRSVIPNA NLRHI LQTG KHY+GSFES+  KS+Q+H+PPFTEDLPRLE PNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180

Query: 181 YYALEDILFEEVEE--RKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY  ED+LFEE+E+  +K+LTWAVIRPN IFGFSPFSLMNIVGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG +  WE F+ ASDADL+AEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMID 360
           IE+YGFEE  ERL+L   MK+K  VW+EIVKENQLQ TK+EE+G WW+ D   S SG ID
Sbjct: 301 IEEYGFEE-GERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 392
           SMNKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]

BLAST of MC11g0269.1 vs. TAIR 10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 639.4 bits (1648), Expect = 1.9e-183
Identity = 294/392 (75.00%), Postives = 334/392 (85.20%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDEP +SFESVAL+IGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
           VARRPRP WNADHPI+YIQCD+SD +D  +KLS L DVTHVFYVTWTNR +E ENCEANG
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
            MLRNVL+++IP A NLRH+ LQTG KHY+G F ++ G    +HDPPFTED+PRL+  NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNVDG---PRHDPPFTEDMPRLQIQNF 180

Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
           YY  EDILFEE+++ + +TW++ RPN IFGFSP+SLMNIVGTLCVYAAICKHEG PL FP
Sbjct: 181 YYTQEDILFEEIKKIETVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFP 240

Query: 241 GNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
           G++  WEGF  ASDADLIAEQ IW AVDPYAKNEAFNCNN D+FKWKH WK+LAEQF IE
Sbjct: 241 GSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIE 300

Query: 301 DYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
           +YGF EE + L L + MK K  VWEE+VKENQLQ+ K+EEVG WWFAD  L   GMIDSM
Sbjct: 301 EYGF-EEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDSM 360

Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
           NKSKE+GFLGFRNS NSFISWIDK+KA+KIVP
Sbjct: 361 NKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]

BLAST of MC11g0269.1 vs. TAIR 10
Match: AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 632.9 bits (1631), Expect = 1.7e-181
Identity = 293/392 (74.74%), Postives = 333/392 (84.95%), Query Frame = 0


Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
           MSWWWAGAIGAA KK +EDEP +SFESVAL+IGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAA-KKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
           VARRPRP WNADHPI+YIQCD+SD +D  +KLS L DVTHVFYVTWTNR +E ENCEANG
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
            MLRNVL+++IP A NLRH+ LQTG KHY+G F ++ G    +HDPPFTED+PRL+  NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNVDG---PRHDPPFTEDMPRLQIQNF 180

Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
           YY  EDILFEE+++ + +TW++ RPN IFGFSP+SLMNIVGTLCVYAAICKHEG PL FP
Sbjct: 181 YYTQEDILFEEIKKIETVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFP 240

Query: 241 GNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
           G++  WEGF  ASDADLIAEQ IW AVDPYAKNEAFNCNN D+FKWKH WK+LAEQF IE
Sbjct: 241 GSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIE 300

Query: 301 DYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
           +YGF EE + L L + MK K  VWEE+VKENQLQ+ K+EEVG WWFAD  L   GMIDSM
Sbjct: 301 EYGF-EEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDSM 360

Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
           NKSKE+GFLGFRNS NSFISWIDK+KA+KIVP
Sbjct: 361 NKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 387

[Close]

BLAST of MC11g0269.1 vs. TAIR 10
Match: AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 236.1 bits (601), Expect = 4.8e-62
Identity = 138/376 (36.70%), Postives = 206/376 (54.79%), Query Frame = 0


Query: 26  ESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYGVARRPRPNWNADHPIEYIQCDISDP 85
           E+VAL+ GVTG+VG  + + L L   PG  W++YGVAR P  N +      +I CD+ + 
Sbjct: 16  ENVALIFGVTGLVGREIVKTL-LMSKPG--WRIYGVARNPEIN-SMTKMYNFISCDLLNA 75

Query: 86  QDAEAKLSQLAD-VTHVFYVTWTNR---STEIENCEANGKMLRNVLRSVIPNATNLRHIS 145
            + + +LS L D V+HVF+VTW+      T+ E C  N  ML N L +++PNA  L+H S
Sbjct: 76  SETKQRLSPLQDIVSHVFWVTWSGEFPLDTD-ECCVQNKTMLMNALDAILPNAKRLKHFS 135

Query: 146 LQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLES-PNFYYALEDILFEEVEERKNLTW 205
           LQTG KHY+   E    +        ++E+ PR  S  NFYY LED+L E++  R ++ W
Sbjct: 136 LQTGMKHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKI-TRSSVVW 195

Query: 206 AVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFPGNRADW-EGFRVASDADLIA 265
           +V RP  + G S  +L N +G+LCVY A+CK+   P  F G R  W E +   SD++L+A
Sbjct: 196 SVQRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLVA 255

Query: 266 EQHIWTAVDPYA--KNEAFNCNNGDVFKWKHFWKVLAEQFNIEDYGFEEEDERLSLEKTM 325
           EQHI+ A       K EAFN  NG  F WK  W  + ++  ++       DE     + M
Sbjct: 256 EQHIFAATSGKVREKGEAFNAINGVGFTWKEIWPEIGKKLGVQVNETTMFDEGFWFGREM 315

Query: 326 KEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTL-SGSGMIDSMNKSKEHGFLGFRNSKN 385
            E+  VW+EIV + +L +T++E++  W+F D        ++    K    GF     + +
Sbjct: 316 VERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYRTLD 375

Query: 386 SFISWIDKFKAYKIVP 393
           S + WID  +  K++P
Sbjct: 376 SVLYWIDVMRDEKLIP 385

The following BLAST results are available for this feature:

BLAST of MC11g0269.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9STX2	2.6e-182	75.00	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP...	[more]
A0A221J5W8	2.9e-173	71.17	(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 ...	[more]
A0A221J5X3	2.7e-171	70.92	(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1...	[more]
A0A1C9CX66	1.5e-169	70.66	(S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC...	[more]
Q6PQJ9	1.4e-167	70.92	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1	[more]

BLAST of MC11g0269.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159803.1	6.29e-301	100.00	3-oxo-Delta(4,5)-steroid 5-beta-reductase [Momordica charantia]	[more]
XP_004152001.1	5.85e-261	85.79	3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus] >KGN58303.1 hypothet...	[more]
XP_022940360.1	2.45e-255	84.77	3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucurbita moschata] >KAG6608438.1 3-o...	[more]
XP_008447374.1	2.01e-254	83.76	PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo] >KAA0037954....	[more]
XP_038899926.1	5.75e-254	84.26	3-oxo-Delta(4,5)-steroid 5-beta-reductase [Benincasa hispida]	[more]

BLAST of MC11g0269.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DZW3	3.04e-301	100.00	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A0A0L8Y0	2.83e-261	85.79	Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G610790 P...	[more]
A0A6J1FQC1	1.18e-255	84.77	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A5A7T3B3	9.72e-255	83.76	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3BHA7	9.72e-255	83.76	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC10348983...	[more]

BLAST of MC11g0269.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24220.1	1.9e-183	75.00	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24220.2	1.7e-181	74.74	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G58750.1	4.8e-62	36.70	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.720		coord: 25..392 e-value: 3.9E-142 score: 475.5
None	No IPR available	PANTHER	PTHR32487:SF20	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE-LIKE	coord: 1..392
None	No IPR available	PANTHER	PTHR32487	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE	coord: 1..392
None	No IPR available	CDD	cd08948	5beta-POR_like_SDR_a	coord: 28..331 e-value: 2.0888E-136 score: 390.068
IPR001509	NAD-dependent epimerase/dehydratase	PFAM	PF01370	Epimerase	coord: 29..212 e-value: 3.8E-6 score: 26.5
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 28..299

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC11g0269	MC11g0269	gene

The following utr5 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g0269.1-utr5	MC11g0269.1-utr5-MC11:2147984..2148351	utr5

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g0269.1-cds	MC11g0269.1-cds-MC11:2148352..2148390	CDS
MC11g0269.1-cds	MC11g0269.1-cds-MC11:2148492..2149631	CDS

The following utr3 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g0269.1-utr3	MC11g0269.1-utr3-MC11:2149632..2149932	utr3

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC11g0269.1	MC11g0269.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009611	response to wounding
biological_process	GO:0008202	steroid metabolic process
biological_process	GO:0010051	xylem and phloem pattern formation
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0047568	3-oxo-5-beta-steroid 4-dehydrogenase activity
molecular_function	GO:0035671	enone reductase activity
molecular_function	GO:0046983	protein dimerization activity