MC11g0255.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC11g0255.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Isoaspartyl peptidase/L-asparaginase
Location	MC11: 2047084 .. 2049097 (+)
Sequence length	1523
RNA-Seq Expression	MC11g0255.1
Synteny	MC11g0255.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAGTTTAAAAGAAAAAAGCTTTAATCATCAGAATCATGACATTCAATGAGTTATATATAAAGTTGCAGATAAAATTCGGAAAAACATTGAATGCAATTAGTAACGGTAAATAAATAGGAAGGAAATAAAAATAAAAAATAAAAAATATAATTGGTATGTAAATTGAGACCCGAAAGTGGCGGAAATATGAGGCCATGTCATAAAAGAAATCGGAATCGAAATTAAAATTGATTTGTATTGGGAAAAGGAAAAGGAAAGGGTGTTGGAGAAATTAGGCTATATAAAGGAGTTGTCCCATTAGTTTGCATCTGGCACAAAGGAAACACAGACTGAGAAAAAGCCAGGGTTTGAGACAGAGAGCGATGGCAGGTTGGGCAATAGCGGTTCACGGAGGGGCCGGCGTGGACCCTAATCTCCCACTCGACCGTCAAGAGGAAGCAAAAAAACTCCTCGCTCGCTGTCTTAATCTTGGCATCGATGCTCTTCGTTCCGATCATCCTGCCATCGATGTCGTCGAACTCGTCGTACGCTCCGCCTTCATCTTTTCATTTTCTTCACTGCCTTTCTTTTATTAGTATTGTTGTCGATGCATATGCATAACCTAATACTAAAATCTCGAATCTCTCCCAATTACTGTTTTGATCCTTCTGGAAATAAGATTCCGTGCATGCACGATTGATTTGGTCCCACCGAGAGGCGATATCGATGATTAGTTACATAATTTTTGGAGGTTTGATTTCCAAATTTGACATTACGAATTTTCTCTTCGGATTTCGAAGGTTAGGGAACTTGAATCGGATCCCCTGTTCAACTCGGGGCGTGGATCTGCGCTGACAGAGGATGGAACCGTGGAGATGGAAGCCAGCATCATGGACGGCCCGAAACGGAGATGCGGGGCGGTGTCCGGATTGACCACTGTTAAAAACCCCATCTCTCTCGCCCGCCTCGTCATGGACAAATCTCCACATTCCTATCTCGCCTTCTCTGGCGCTGAGGAATTCGCCAGGCAACAGGTTTCGTTTCGTTTCGTTTCCCCAAATTTTCCCTATTCAACATTGAGACTGCCGAAATCCCGTCGATTAATAAGGCTGACTTTTTTAACACTTTCGACTGGGGATCGTGCAGGGCGTGGAGATGGTGTGCAATGACTATTTCATCACCGAAGAAAACGTGGGAATGCTTAAACTAGCCAAAGAAGCCAACAGTATACTGGTACGAATTTTATTTGAACTGCAACAGTAACCGTTCATTGAGAGAAAAAGAACCACGTGGGCAGGCGGACGCCAAATGACTTTTTTTTTTCCCCTTTTTCTAATTGTTGGGTTGGTTGGAACAGTTCGATTATAGGATCCCAAACGCAGGATTAGAGAAGTGTAGCGCAAGCGTGGAGACGCCGTTGCAAATGAACGGCCTCCCAATCAGCGTGTACGCGCCGGAGACGGTTGGGTGCGTGGTGGTGGACAGCCAAGGAAGGTGCGCGGCGGCCACGTCAACCGGCGGGCTGATGAACAAACGGAGCGGCCGCATCGGGGACTCGCCGCTGATCGGCGCAGGGACGTACGCGTGCGGGTTGTGCGGGGTATCGTGTACGGGAGAAGGGGAGGCGATCATAAGAGGAACACTGGCGAGGGAAGTGGCGGCGGTGATGGAGTACAAAGGGATGGGACTGCAGGAGGCAGTGAGCTACGTGGTAGAGGAGCGACTGGACGAAGGCAAGGCAGGGCTCATCGCAGTTTCATCCAATGGGGAAGTGGCCTGTGGATTCAACACCACCGGAATGTTCAGAGGATGCGCGACGGAGGCTGGCTTTATGGAGGTTGGAATTTGGTAATCGATAGTTAGTTTTCTTTTTTGCTTTTTTTTTTTTTTAATTATGGAACGAAGTTTGTGGGTTAATTTTTAATTAGTGCTGTGTAACTGCTGAACTAAATAATCTTCTGGATTTTTAAAATTCAGAGGGTGCTATGCGATAGATGACCAATGTGTCAGCAATAAATTGCCCTTGGCCCGAAC

mRNA sequence

AAAGTTTAAAAGAAAAAAGCTTTAATCATCAGAATCATGACATTCAATGAGTTATATATAAAGTTGCAGATAAAATTCGGAAAAACATTGAATGCAATTAGTAACGGTAAATAAATAGGAAGGAAATAAAAATAAAAAATAAAAAATATAATTGGTATGTAAATTGAGACCCGAAAGTGGCGGAAATATGAGGCCATGTCATAAAAGAAATCGGAATCGAAATTAAAATTGATTTGTATTGGGAAAAGGAAAAGGAAAGGGTGTTGGAGAAATTAGGCTATATAAAGGAGTTGTCCCATTAGTTTGCATCTGGCACAAAGGAAACACAGACTGAGAAAAAGCCAGGGTTTGAGACAGAGAGCGATGGCAGGTTGGGCAATAGCGGTTCACGGAGGGGCCGGCGTGGACCCTAATCTCCCACTCGACCGTCAAGAGGAAGCAAAAAAACTCCTCGCTCGCTGTCTTAATCTTGGCATCGATGCTCTTCGTTCCGATCATCCTGCCATCGATGTCGTCGAACTCGTCGTTAGGGAACTTGAATCGGATCCCCTGTTCAACTCGGGGCGTGGATCTGCGCTGACAGAGGATGGAACCGTGGAGATGGAAGCCAGCATCATGGACGGCCCGAAACGGAGATGCGGGGCGGTGTCCGGATTGACCACTGTTAAAAACCCCATCTCTCTCGCCCGCCTCGTCATGGACAAATCTCCACATTCCTATCTCGCCTTCTCTGGCGCTGAGGAATTCGCCAGGCAACAGGGCGTGGAGATGGTGTGCAATGACTATTTCATCACCGAAGAAAACGTGGGAATGCTTAAACTAGCCAAAGAAGCCAACAGTATACTGTTCGATTATAGGATCCCAAACGCAGGATTAGAGAAGTGTAGCGCAAGCGTGGAGACGCCGTTGCAAATGAACGGCCTCCCAATCAGCGTGTACGCGCCGGAGACGGTTGGGTGCGTGGTGGTGGACAGCCAAGGAAGGTGCGCGGCGGCCACGTCAACCGGCGGGCTGATGAACAAACGGAGCGGCCGCATCGGGGACTCGCCGCTGATCGGCGCAGGGACGTACGCGTGCGGGTTGTGCGGGGTATCGTGTACGGGAGAAGGGGAGGCGATCATAAGAGGAACACTGGCGAGGGAAGTGGCGGCGGTGATGGAGTACAAAGGGATGGGACTGCAGGAGGCAGTGAGCTACGTGGTAGAGGAGCGACTGGACGAAGGCAAGGCAGGGCTCATCGCAGTTTCATCCAATGGGGAAGTGGCCTGTGGATTCAACACCACCGGAATGTTCAGAGGATGCGCGACGGAGGCTGGCTTTATGGAGGTTGGAATTTGGTAATCGATAGTTAGTTTTCTTTTTTGCTTTTTTTTTTTTTTAATTATGGAACGAAGTTTGTGGGTTAATTTTTAATTAGTGCTGTGTAACTGCTGAACTAAATAATCTTCTGGATTTTTAAAATTCAGAGGGTGCTATGCGATAGATGACCAATGTGTCAGCAATAAATTGCCCTTGGCCCGAAC

Coding sequence (CDS)

ATGGCAGGTTGGGCAATAGCGGTTCACGGAGGGGCCGGCGTGGACCCTAATCTCCCACTCGACCGTCAAGAGGAAGCAAAAAAACTCCTCGCTCGCTGTCTTAATCTTGGCATCGATGCTCTTCGTTCCGATCATCCTGCCATCGATGTCGTCGAACTCGTCGTTAGGGAACTTGAATCGGATCCCCTGTTCAACTCGGGGCGTGGATCTGCGCTGACAGAGGATGGAACCGTGGAGATGGAAGCCAGCATCATGGACGGCCCGAAACGGAGATGCGGGGCGGTGTCCGGATTGACCACTGTTAAAAACCCCATCTCTCTCGCCCGCCTCGTCATGGACAAATCTCCACATTCCTATCTCGCCTTCTCTGGCGCTGAGGAATTCGCCAGGCAACAGGGCGTGGAGATGGTGTGCAATGACTATTTCATCACCGAAGAAAACGTGGGAATGCTTAAACTAGCCAAAGAAGCCAACAGTATACTGTTCGATTATAGGATCCCAAACGCAGGATTAGAGAAGTGTAGCGCAAGCGTGGAGACGCCGTTGCAAATGAACGGCCTCCCAATCAGCGTGTACGCGCCGGAGACGGTTGGGTGCGTGGTGGTGGACAGCCAAGGAAGGTGCGCGGCGGCCACGTCAACCGGCGGGCTGATGAACAAACGGAGCGGCCGCATCGGGGACTCGCCGCTGATCGGCGCAGGGACGTACGCGTGCGGGTTGTGCGGGGTATCGTGTACGGGAGAAGGGGAGGCGATCATAAGAGGAACACTGGCGAGGGAAGTGGCGGCGGTGATGGAGTACAAAGGGATGGGACTGCAGGAGGCAGTGAGCTACGTGGTAGAGGAGCGACTGGACGAAGGCAAGGCAGGGCTCATCGCAGTTTCATCCAATGGGGAAGTGGCCTGTGGATTCAACACCACCGGAATGTTCAGAGGATGCGCGACGGAGGCTGGCTTTATGGAGGTTGGAATTTGGTAA

Protein sequence

MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELESDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYLAFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVETPLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGLCGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEVACGFNTTGMFRGCATEAGFMEVGIW

Homology

[Close]

BLAST of MC11g0255.1 vs. ExPASy Swiss-Prot
Match: Q8GXG1 (Probable isoaspartyl peptidase/L-asparaginase 2 OS=Arabidopsis thaliana OX=3702 GN=At3g16150 PE=2 SV=2)

HSP 1 Score: 531.2 bits (1367), Expect = 8.3e-150
Identity = 263/325 (80.92%), Postives = 291/325 (89.54%), Query Frame = 0


Query: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60
           M GWAIAVHGGAG+DPNLP +RQEEAK+LL RCLNLGI ALRS+  AIDVVELV+RELE+
Sbjct: 1   MGGWAIAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE GTVEMEASIMDG KRRCGAVSG+TTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180
           AFSGAE+FAR+QGVE+V N+YF+T++NVGMLKLAKEANSILFDYRIP  G    +A+ ++
Sbjct: 121 AFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAG-AAATDS 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240
           P+QMNGLPIS+YAPETVGCVVVD +G CAA TSTGGLMNK  GRIGDSPLIGAGTYA   
Sbjct: 181 PIQMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEF 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIR TLAR+V+AVMEYKG+ LQEAV YV++ RLDEG AGLIAVS+ GEV
Sbjct: 241 CGVSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEV 300

Query: 301 ACGFNTTGMFRGCATEAGFMEVGIW 326
            CGFN+ GMFRGCATE GFMEV IW
Sbjct: 301 VCGFNSNGMFRGCATEDGFMEVAIW 324

[Close]

BLAST of MC11g0255.1 vs. ExPASy Swiss-Prot
Match: P50287 (Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08100 PE=1 SV=2)

HSP 1 Score: 366.7 bits (940), Expect = 2.7e-100
Identity = 193/326 (59.20%), Postives = 232/326 (71.17%), Query Frame = 0


Query: 1   MAGWAIAVHGGAGVDP-NLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELE 60
           M GWAIA+HGGAG  P +LP +R+   +  L  CL+LGI AL+S  P +DV ELVVRELE
Sbjct: 1   MVGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELE 60

Query: 61  SDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
           + P FN+G+GS LT  GTVEMEASIMDG  +RCGAVSGLTTV NPISLARLVM+K+PH Y
Sbjct: 61  NHPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIY 120

Query: 121 LAFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVE 180
           LAF  AE FAR  GVE V + +FIT EN+  LK AKE N +  DY +P         S +
Sbjct: 121 LAFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVP---------SPK 180

Query: 181 TPLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACG 240
            P       I      TVGCV VDS G  A+ATSTGG +NK  GRIGD+P+IGAGTYA  
Sbjct: 181 VPDNCGDSQIG-----TVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANH 240

Query: 241 LCGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGE 300
           LC +S TG+GE IIRGT+AR+VAA+MEYKG+ L EA +YVV++ +  G  GL+AVS+NGE
Sbjct: 241 LCAISATGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGE 300

Query: 301 VACGFNTTGMFRGCATEAGFMEVGIW 326
           V   FNTTGMFR CA+E G+ E+ IW
Sbjct: 301 VTMPFNTTGMFRACASEDGYSEIAIW 312

[Close]

BLAST of MC11g0255.1 vs. ExPASy Swiss-Prot
Match: P30364 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 7.9e-100
Identity = 197/327 (60.24%), Postives = 237/327 (72.48%), Query Frame = 0


Query: 1   MAGWAIAVHGGAGVDP-NLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELE 60
           M GW+IA+HGGAG  P +LP +R++  ++ L  CL +G++AL+S  P +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRQPREEGLRHCLQIGVEALKSQKPPLDVVELVVRELE 60

Query: 61  SDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
           +   FN+G GS LT  GTVEMEASIMDG   +CGAVSGL+TV NPISLARLVMDK+PH Y
Sbjct: 61  NIQHFNAGIGSVLTNSGTVEMEASIMDGKTMKCGAVSGLSTVLNPISLARLVMDKTPHIY 120

Query: 121 LAFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPN-AGLEKCSASV 180
           LAF GA++FA+QQGVE V + +FIT ENV  LKLA EAN +  DY   N     +  A  
Sbjct: 121 LAFQGAQDFAKQQGVETVDSSHFITAENVERLKLAIEANRVQVDYSQYNYPQPAQDDAEK 180

Query: 181 ETPLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYAC 240
           E PL      I      TVGCV VDS G  A+ATSTGGL+NK  GRIGD+PLIGAGTYA 
Sbjct: 181 ELPLANGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 GLCGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNG 300
            LC VS TG+GEAII  T+AR+VAA+ME+KG+ L+EA  YVV ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEAIISATVARDVAALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAG 300

Query: 301 EVACGFNTTGMFRGCATEAGFMEVGIW 326
           E+A  FNTTGMFR  ATE G+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRASATEDGYSEIAIW 322

[Close]

BLAST of MC11g0255.1 vs. ExPASy Swiss-Prot
Match: Q9ZSD6 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.4e-99
Identity = 195/327 (59.63%), Postives = 237/327 (72.48%), Query Frame = 0


Query: 1   MAGWAIAVHGGAGVDP-NLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELE 60
           M GW+IA+HGGAG  P +LP +R++  ++ L  CL +G++AL++  P +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGVEALKAQKPPLDVVELVVRELE 60

Query: 61  SDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
           +   FN+G GS LT  GTVEMEASIMDG   +CGAVSGL+TV NPISLARLVMDK+PH Y
Sbjct: 61  NIEHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLSTVLNPISLARLVMDKTPHIY 120

Query: 121 LAFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPN-AGLEKCSASV 180
           LAF GA++FA+QQGVE V + + IT ENV  LKLA EAN +  DY   N     K  A  
Sbjct: 121 LAFQGAQDFAKQQGVETVDSSHLITAENVERLKLAIEANRVQVDYSQYNYPEPVKDDAEK 180

Query: 181 ETPLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYAC 240
           E PL      I      TVGCV VDS G  A+ATSTGGL+NK  GRIGD+PLIGAGTYA 
Sbjct: 181 ELPLTNGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 GLCGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNG 300
            LC VS TG+GE IIR T+AR+VAA+ME+KG+ L+EA  +V+ ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEEIIRATVARDVAALMEFKGLSLKEAADFVIHERTPKGTVGLIAVSAAG 300

Query: 301 EVACGFNTTGMFRGCATEAGFMEVGIW 326
           E+A  FNTTGMFR CATE G+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRACATEDGYSEIAIW 322

[Close]

BLAST of MC11g0255.1 vs. ExPASy Swiss-Prot
Match: P50288 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 8.8e-99
Identity = 195/327 (59.63%), Postives = 236/327 (72.17%), Query Frame = 0


Query: 1   MAGWAIAVHGGAGVDP-NLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELE 60
           M GW+IA+HGGAG  P +LP +R++  ++ L  CL +GI+AL++ +P +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGIEALKAQNPPLDVVELVVRELE 60

Query: 61  SDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
           +   FN+G GS LT  GTVEMEASIMDG   +CGAVSGL TV NPISLAR VMDK+PH +
Sbjct: 61  NIQHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLNTVMNPISLARQVMDKTPHIF 120

Query: 121 LAFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPN-AGLEKCSASV 180
           LAF GA++  +QQGVE V + +FITEENV  LKLA EAN +  DY   N     +  A  
Sbjct: 121 LAFQGAQDLGKQQGVETVDSSHFITEENVERLKLAIEANRVQVDYSQYNYTQPVQDDAEK 180

Query: 181 ETPLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYAC 240
           E PL      I      TVGCV VDS G  A+ATSTGGL+NK  GRIGD+PLIGAGTYA 
Sbjct: 181 ELPLANGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 GLCGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNG 300
            LC VS TG+GEAIIR T+AR+VAA+ME+KG+ L+EA   VV ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEAIIRATVARDVAALMEFKGLSLKEAADCVVHERTPKGTVGLIAVSAAG 300

Query: 301 EVACGFNTTGMFRGCATEAGFMEVGIW 326
           E+A  FNTTGMFR CATE G+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRACATEDGYSEIAIW 322

[Close]

BLAST of MC11g0255.1 vs. NCBI nr
Match: XP_022141340.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Momordica charantia])

HSP 1 Score: 646 bits (1666), Expect = 1.09e-233
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 0


Query: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60
           MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES
Sbjct: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180
           AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET
Sbjct: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240
           PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL
Sbjct: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300

Query: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325
           ACGFNTTGMFRGCATEAGFMEVGIW
Sbjct: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325

[Close]

BLAST of MC11g0255.1 vs. NCBI nr
Match: XP_022982271.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita maxima])

HSP 1 Score: 582 bits (1500), Expect = 2.03e-208
Identity = 290/325 (89.23%), Postives = 309/325 (95.08%), Query Frame = 0


Query: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60
           M GWAIAVHGGAGVDPNLPL RQE+AK LL RCL+LGI ALRS+  AIDVVELVVRELE+
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180
           AFSGAE+FAR+QGVE+ CNDYF+TEENVGML+LA+EANSI  DYR+PN   EKCSASVET
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPN---EKCSASVET 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240
           PLQMNGLPISVYAPETVGCVVVDS+GRCAAATSTGGLMNK+ GRIGDSPLIG+GTYACG+
Sbjct: 181 PLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGV 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEYKG+GLQEAV+YV+EERLDEGKAGLIAVSSNGEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV 300

Query: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325
            CGFNTTGMFRGCATE GFMEVGIW
Sbjct: 301 VCGFNTTGMFRGCATEDGFMEVGIW 322

[Close]

BLAST of MC11g0255.1 vs. NCBI nr
Match: XP_022941449.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita moschata] >KAG6608447.1 putative isoaspartyl peptidase/L-asparaginase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037783.1 putative isoaspartyl peptidase/L-asparaginase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 582 bits (1499), Expect = 2.88e-208
Identity = 290/325 (89.23%), Postives = 308/325 (94.77%), Query Frame = 0


Query: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60
           M GWAIAVHGGAGVDPNLPL RQE+AK LL RCL+LGI ALRS+  AIDVVELVVRELE+
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180
           AFSGAE+FAR+QGVE+ CNDYF+TEENVGML+LA+EANSI  DYR+PN   EKCS SVET
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPN---EKCSTSVET 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240
           PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNK+ GRIGDSPLIG+GTYACG+
Sbjct: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGV 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEYKG+GLQEAV+YV+EERLDEGKAGLIAVSSNGEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV 300

Query: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325
            CGFNTTGMFRGCATE GFMEVGIW
Sbjct: 301 VCGFNTTGMFRGCATEDGFMEVGIW 322

[Close]

BLAST of MC11g0255.1 vs. NCBI nr
Match: XP_023525179.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 581 bits (1498), Expect = 4.25e-208
Identity = 290/325 (89.23%), Postives = 308/325 (94.77%), Query Frame = 0


Query: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60
           M GWAIAVHGGAGVDPNLPL RQE+AK LL RCL+LGI ALRS+  AIDVVELVVRELE+
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNSSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180
           AFSGAE+FAR+QGVE+ CNDYF+TEENVGML+LA+EANSI  DYR+P    EKCSASVET
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPTN--EKCSASVET 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240
           PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNK+ GRIGDSPLIG+GTYACG+
Sbjct: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGV 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEYKG+GLQEAV+YV+EERLDEGKAGLIAVSSNGEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV 300

Query: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325
            CGFNTTGMFRGCATE GFMEVGIW
Sbjct: 301 VCGFNTTGMFRGCATEDGFMEVGIW 323

[Close]

BLAST of MC11g0255.1 vs. NCBI nr
Match: KAF3446610.1 (hypothetical protein FNV43_RR11790 [Rhamnella rubrinervis])

HSP 1 Score: 579 bits (1492), Expect = 3.76e-207
Identity = 289/325 (88.92%), Postives = 307/325 (94.46%), Query Frame = 0


Query: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60
           M GWAIAVHGGAGVDPNLPL+RQEEAK+LL RCLNLGI ALRS+  AIDVVELVVRELE+
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLERQEEAKQLLKRCLNLGISALRSNLSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DP+FNSGRGSALTE GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPVFNSGRGSALTEKGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180
           AFSGAE+FARQQGVE+V N+YFIT ENVGMLKLAKEAN+ILFDYR+PNAGLE CSA VE 
Sbjct: 121 AFSGAEDFARQQGVEVVENEYFITNENVGMLKLAKEANAILFDYRLPNAGLESCSAGVER 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240
           PL MNGLPISVYAPETVGCVVVD+QGRCAAATSTGGLMNK +GRIGDSPLIGAGTYAC L
Sbjct: 181 PLVMNGLPISVYAPETVGCVVVDNQGRCAAATSTGGLMNKMTGRIGDSPLIGAGTYACNL 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEY+G+GLQEAV +V++ERLDEG AGLIAVS+ GEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYRGLGLQEAVDFVIKERLDEGHAGLIAVSNKGEV 300

Query: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325
           ACGFNTTGMFRGCATE GFMEVGIW
Sbjct: 301 ACGFNTTGMFRGCATEDGFMEVGIW 325

[Close]

BLAST of MC11g0255.1 vs. ExPASy TrEMBL
Match: A0A6J1CHT1 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Momordica charantia OX=3673 GN=LOC111011731 PE=4 SV=1)

HSP 1 Score: 646 bits (1666), Expect = 5.30e-234
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 0


Query: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60
           MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES
Sbjct: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180
           AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET
Sbjct: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240
           PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL
Sbjct: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300

Query: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325
           ACGFNTTGMFRGCATEAGFMEVGIW
Sbjct: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325

[Close]

BLAST of MC11g0255.1 vs. ExPASy TrEMBL
Match: A0A6J1IYV7 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita maxima OX=3661 GN=LOC111481149 PE=4 SV=1)

HSP 1 Score: 582 bits (1500), Expect = 9.83e-209
Identity = 290/325 (89.23%), Postives = 309/325 (95.08%), Query Frame = 0


Query: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60
           M GWAIAVHGGAGVDPNLPL RQE+AK LL RCL+LGI ALRS+  AIDVVELVVRELE+
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180
           AFSGAE+FAR+QGVE+ CNDYF+TEENVGML+LA+EANSI  DYR+PN   EKCSASVET
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPN---EKCSASVET 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240
           PLQMNGLPISVYAPETVGCVVVDS+GRCAAATSTGGLMNK+ GRIGDSPLIG+GTYACG+
Sbjct: 181 PLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGV 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEYKG+GLQEAV+YV+EERLDEGKAGLIAVSSNGEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV 300

Query: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325
            CGFNTTGMFRGCATE GFMEVGIW
Sbjct: 301 VCGFNTTGMFRGCATEDGFMEVGIW 322

[Close]

BLAST of MC11g0255.1 vs. ExPASy TrEMBL
Match: A0A6J1FND2 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita moschata OX=3662 GN=LOC111446743 PE=4 SV=1)

HSP 1 Score: 582 bits (1499), Expect = 1.40e-208
Identity = 290/325 (89.23%), Postives = 308/325 (94.77%), Query Frame = 0


Query: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60
           M GWAIAVHGGAGVDPNLPL RQE+AK LL RCL+LGI ALRS+  AIDVVELVVRELE+
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180
           AFSGAE+FAR+QGVE+ CNDYF+TEENVGML+LA+EANSI  DYR+PN   EKCS SVET
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPN---EKCSTSVET 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240
           PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNK+ GRIGDSPLIG+GTYACG+
Sbjct: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGV 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEYKG+GLQEAV+YV+EERLDEGKAGLIAVSSNGEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV 300

Query: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325
            CGFNTTGMFRGCATE GFMEVGIW
Sbjct: 301 VCGFNTTGMFRGCATEDGFMEVGIW 322

[Close]

BLAST of MC11g0255.1 vs. ExPASy TrEMBL
Match: A0A2P5BJ92 (Peptidase T2, asparaginase OS=Parasponia andersonii OX=3476 GN=PanWU01x14_233900 PE=4 SV=1)

HSP 1 Score: 578 bits (1489), Expect = 5.21e-207
Identity = 287/325 (88.31%), Postives = 308/325 (94.77%), Query Frame = 0


Query: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60
           M GWAIAVHGGAGVDPNLPL+RQE+AK+LL RCLNLGI ALRS+ PAIDVVELVVRELE+
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLERQEQAKQLLTRCLNLGISALRSNLPAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DP FNSGRGSALTE GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVM+KSPHSYL
Sbjct: 61  DPFFNSGRGSALTEKGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMEKSPHSYL 120

Query: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180
           AFSGAE+FARQQGVE+V N+YFIT+ENVGMLKLAKEA +ILFDYRIP AGLE CSA V++
Sbjct: 121 AFSGAEDFARQQGVEVVDNEYFITKENVGMLKLAKEAKTILFDYRIPTAGLETCSAGVDS 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240
           P+ MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNK +GRIGDSPLIGAGTYAC L
Sbjct: 181 PIVMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKMTGRIGDSPLIGAGTYACDL 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEYKG+GLQEAV +V+++RLDEG+AGLIAVS NGEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVDFVIKQRLDEGQAGLIAVSRNGEV 300

Query: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325
           ACGFNTTGMFRGCATE GFMEVGIW
Sbjct: 301 ACGFNTTGMFRGCATEDGFMEVGIW 325

[Close]

BLAST of MC11g0255.1 vs. ExPASy TrEMBL
Match: A0A2P5EGN6 (Peptidase T2, asparaginase OS=Trema orientale OX=63057 GN=TorRG33x02_195160 PE=4 SV=1)

HSP 1 Score: 575 bits (1481), Expect = 8.63e-206
Identity = 286/325 (88.00%), Postives = 306/325 (94.15%), Query Frame = 0


Query: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60
           M GWAIAVHGGAGVDPNLPL+ QE+AK+LL RCLNLGI ALRS+ PAIDVVELVVRELE+
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLECQEQAKQLLTRCLNLGISALRSNLPAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DP FNSGRGSALTE GTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVM+KSPHSYL
Sbjct: 61  DPFFNSGRGSALTEKGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMEKSPHSYL 120

Query: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180
           AFSGAE+FARQQGVE+V N+YFIT+ENVGMLKLAKEA +ILFDYRIP  GLEKCSA V++
Sbjct: 121 AFSGAEDFARQQGVEVVDNEYFITKENVGMLKLAKEAKTILFDYRIPTVGLEKCSAGVDS 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240
           P+ MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNK +GRIGDSPLIGAGTYAC L
Sbjct: 181 PIVMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKMTGRIGDSPLIGAGTYACDL 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEYKG+GLQEAV +V++ RLDEG+AGLIAVS NGEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVDFVIKHRLDEGQAGLIAVSRNGEV 300

Query: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325
           ACGFNTTGMFRGCATE GFMEVGIW
Sbjct: 301 ACGFNTTGMFRGCATEDGFMEVGIW 325

[Close]

BLAST of MC11g0255.1 vs. TAIR 10
Match: AT3G16150.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 531.2 bits (1367), Expect = 5.9e-151
Identity = 263/325 (80.92%), Postives = 291/325 (89.54%), Query Frame = 0


Query: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60
           M GWAIAVHGGAG+DPNLP +RQEEAK+LL RCLNLGI ALRS+  AIDVVELV+RELE+
Sbjct: 1   MGGWAIAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELET 60

Query: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE GTVEMEASIMDG KRRCGAVSG+TTVKNPISLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180
           AFSGAE+FAR+QGVE+V N+YF+T++NVGMLKLAKEANSILFDYRIP  G    +A+ ++
Sbjct: 121 AFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAG-AAATDS 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240
           P+QMNGLPIS+YAPETVGCVVVD +G CAA TSTGGLMNK  GRIGDSPLIGAGTYA   
Sbjct: 181 PIQMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEF 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIR TLAR+V+AVMEYKG+ LQEAV YV++ RLDEG AGLIAVS+ GEV
Sbjct: 241 CGVSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEV 300

Query: 301 ACGFNTTGMFRGCATEAGFMEVGIW 326
            CGFN+ GMFRGCATE GFMEV IW
Sbjct: 301 VCGFNSNGMFRGCATEDGFMEVAIW 324

[Close]

BLAST of MC11g0255.1 vs. TAIR 10
Match: AT5G08100.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 366.7 bits (940), Expect = 1.9e-101
Identity = 193/326 (59.20%), Postives = 232/326 (71.17%), Query Frame = 0


Query: 1   MAGWAIAVHGGAGVDP-NLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELE 60
           M GWAIA+HGGAG  P +LP +R+   +  L  CL+LGI AL+S  P +DV ELVVRELE
Sbjct: 1   MVGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELE 60

Query: 61  SDPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSY 120
           + P FN+G+GS LT  GTVEMEASIMDG  +RCGAVSGLTTV NPISLARLVM+K+PH Y
Sbjct: 61  NHPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIY 120

Query: 121 LAFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVE 180
           LAF  AE FAR  GVE V + +FIT EN+  LK AKE N +  DY +P         S +
Sbjct: 121 LAFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVP---------SPK 180

Query: 181 TPLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACG 240
            P       I      TVGCV VDS G  A+ATSTGG +NK  GRIGD+P+IGAGTYA  
Sbjct: 181 VPDNCGDSQIG-----TVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANH 240

Query: 241 LCGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGE 300
           LC +S TG+GE IIRGT+AR+VAA+MEYKG+ L EA +YVV++ +  G  GL+AVS+NGE
Sbjct: 241 LCAISATGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGE 300

Query: 301 VACGFNTTGMFRGCATEAGFMEVGIW 326
           V   FNTTGMFR CA+E G+ E+ IW
Sbjct: 301 VTMPFNTTGMFRACASEDGYSEIAIW 312

[Close]

BLAST of MC11g0255.1 vs. TAIR 10
Match: AT5G08100.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 278.1 bits (710), Expect = 9.0e-75
Identity = 146/246 (59.35%), Postives = 174/246 (70.73%), Query Frame = 0


Query: 80  MEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYLAFSGAEEFARQQGVEMVCN 139
           MEASIMDG  +RCGAVSGLTTV NPISLARLVM+K+PH YLAF  AE FAR  GVE V +
Sbjct: 1   MEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDAAEAFARAHGVETVDS 60

Query: 140 DYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVETPLQMNGLPISVYAPETVGC 199
            +FIT EN+  LK AKE N +  DY +P         S + P       I      TVGC
Sbjct: 61  SHFITPENIARLKQAKEFNRVQLDYTVP---------SPKVPDNCGDSQIG-----TVGC 120

Query: 200 VVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGLCGVSCTGEGEAIIRGTLAR 259
           V VDS G  A+ATSTGG +NK  GRIGD+P+IGAGTYA  LC +S TG+GE IIRGT+AR
Sbjct: 121 VAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKGEDIIRGTVAR 180

Query: 260 EVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEVACGFNTTGMFRGCATEAGF 319
           +VAA+MEYKG+ L EA +YVV++ +  G  GL+AVS+NGEV   FNTTGMFR CA+E G+
Sbjct: 181 DVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGMFRACASEDGY 232

Query: 320 MEVGIW 326
            E+ IW
Sbjct: 241 SEIAIW 232

[Close]

BLAST of MC11g0255.1 vs. TAIR 10
Match: AT4G00590.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 104.4 bits (259), Expect = 1.8e-22
Identity = 93/336 (27.68%), Postives = 142/336 (42.26%), Query Frame = 0


Query: 6   IAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDH-PAIDVVELVVRELESDPLF 65
           +AVH GAG         ++  + ++ R        LR D    ID V   ++ LE DP  
Sbjct: 15  VAVHVGAGYH---AAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPST 74

Query: 66  NSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARL----------VMDK 125
           N+GRGS LTEDG VE +AS+MDG     G V  +  V+N I +A L          ++ +
Sbjct: 75  NAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKEQITGSTLLGR 134

Query: 126 SPHSYLAFSGAEEFARQQ-----GVEMVCNDYFITEENVGMLKLAKE------ANSILFD 185
            P   L   GA  + + +     G     + + +TE      +  K       A SIL  
Sbjct: 135 IPPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSA 194

Query: 186 YRIPNA--GLEKCSASVETPLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKR 245
              P      E C  +V +    +   I     +TVG + VD++G  A  +S+GG+  K 
Sbjct: 195 EEHPRGTENNETCEENVSSCAAADEDKIM----DTVGVICVDNEGHIACGSSSGGIAMKI 254

Query: 246 SGRIGDSPLIGAGTYACG--------LCGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQ 301
           SGR+G +   G+G +A          L G   +G GE ++RG  ARE    +     G  
Sbjct: 255 SGRVGLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPA 314

[Close]

BLAST of MC11g0255.1 vs. TAIR 10
Match: AT4G00590.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 93.6 bits (231), Expect = 3.2e-19
Identity = 91/328 (27.74%), Postives = 141/328 (42.99%), Query Frame = 0


Query: 6   IAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDH-PAIDVVELVVRELESDPLF 65
           +AVH GAG         ++  + ++ R        LR D    ID V   ++ LE DP  
Sbjct: 15  VAVHVGAGYH---AAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPST 74

Query: 66  NSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPIS--LARLVMDKSPHSYLAF 125
           N+GRGS LTEDG VE +AS+MDG     G   G+  V + +S  L++L++       L  
Sbjct: 75  NAGRGSNLTEDGHVECDASLMDGDS---GMFGGVGAVPDQVSEMLSKLLL----CWLLVG 134

Query: 126 SGAEEFARQQ-----GVEMVCNDYFITEENVGMLKLAKE------ANSILFDYRIPNA-- 185
            GA  + + +     G     + + +TE      +  K       A SIL     P    
Sbjct: 135 EGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSAEEHPRGTE 194

Query: 186 GLEKCSASVETPLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSP 245
             E C  +V +    +   I     +TVG + VD++G  A  +S+GG+  K SGR+G + 
Sbjct: 195 NNETCEENVSSCAAADEDKIM----DTVGVICVDNEGHIACGSSSGGIAMKISGRVGLAA 254

Query: 246 LIGAGTYACG--------LCGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVE 301
             G+G +A          L G   +G GE ++RG  ARE    +     G   A   V+ 
Sbjct: 255 TYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPASAAMKVLR 314

The following BLAST results are available for this feature:

BLAST of MC11g0255.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GXG1	8.3e-150	80.92	Probable isoaspartyl peptidase/L-asparaginase 2 OS=Arabidopsis thaliana OX=3702 ...	[more]
P50287	2.7e-100	59.20	Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08...	[more]
P30364	7.9e-100	60.24	Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1	[more]
Q9ZSD6	1.4e-99	59.63	Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1	[more]
P50288	8.8e-99	59.63	Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1	[more]

BLAST of MC11g0255.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141340.1	1.09e-233	100.00	probable isoaspartyl peptidase/L-asparaginase 2 [Momordica charantia]	[more]
XP_022982271.1	2.03e-208	89.23	probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita maxima]	[more]
XP_022941449.1	2.88e-208	89.23	probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita moschata] >KAG6608447...	[more]
XP_023525179.1	4.25e-208	89.23	probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita pepo subsp. pepo]	[more]
KAF3446610.1	3.76e-207	88.92	hypothetical protein FNV43_RR11790 [Rhamnella rubrinervis]	[more]

BLAST of MC11g0255.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CHT1	5.30e-234	100.00	probable isoaspartyl peptidase/L-asparaginase 2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1IYV7	9.83e-209	89.23	probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1FND2	1.40e-208	89.23	probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A2P5BJ92	5.21e-207	88.31	Peptidase T2, asparaginase OS=Parasponia andersonii OX=3476 GN=PanWU01x14_233900...	[more]
A0A2P5EGN6	8.63e-206	88.00	Peptidase T2, asparaginase OS=Trema orientale OX=63057 GN=TorRG33x02_195160 PE=4...	[more]

BLAST of MC11g0255.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G16150.1	5.9e-151	80.92	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT5G08100.1	1.9e-101	59.20	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT5G08100.2	9.0e-75	59.35	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT4G00590.1	1.8e-22	27.68	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT4G00590.2	3.2e-19	27.74	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000246	Peptidase T2, asparaginase 2	PFAM	PF01112	Asparaginase_2	coord: 4..325 e-value: 1.0E-101 score: 340.1
IPR000246	Peptidase T2, asparaginase 2	PANTHER	PTHR10188	L-ASPARAGINASE	coord: 1..325
None	No IPR available	GENE3D	3.60.20.30	(Glycosyl)asparaginase	coord: 196..325 e-value: 2.6E-42 score: 146.1
None	No IPR available	PIRSR	PIRSR600246-1	PIRSR600246-1	coord: 2..316 e-value: 5.4E-91 score: 302.9
None	No IPR available	PIRSR	PIRSR600246-2	PIRSR600246-2	coord: 2..316 e-value: 5.4E-91 score: 302.9
None	No IPR available	PIRSR	PIRSR600246-3	PIRSR600246-3	coord: 2..316 e-value: 5.4E-91 score: 302.9
None	No IPR available	PANTHER	PTHR10188:SF36	PEPTIDASE T2, ASPARAGINASE 2, NUCLEOPHILE AMINOHYDROLASE-RELATED	coord: 1..325
None	No IPR available	CDD	cd04701	Asparaginase_2	coord: 5..318 e-value: 1.13242E-121 score: 348.297
IPR029055	Nucleophile aminohydrolases, N-terminal	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 4..322

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC11g0255	MC11g0255	gene

The following utr5 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g0255.1-utr5	MC11g0255.1-utr5-MC11:2047084..2047446	utr5

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g0255.1-cds	MC11g0255.1-cds-MC11:2047447..2047608	CDS
MC11g0255.1-cds	MC11g0255.1-cds-MC11:2047864..2048097	CDS
MC11g0255.1-cds	MC11g0255.1-cds-MC11:2048210..2048296	CDS
MC11g0255.1-cds	MC11g0255.1-cds-MC11:2048421..2048915	CDS

The following utr3 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g0255.1-utr3	MC11g0255.1-utr3-MC11:2048916..2049097	utr3

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC11g0255.1	MC11g0255.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016540	protein autoprocessing
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0004067	asparaginase activity
molecular_function	GO:0008798	beta-aspartyl-peptidase activity