MC11g0240.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC11g0240.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RING-type domain-containing protein
Location	MC11: 1906680 .. 1909991 (+)
Sequence length	1645
RNA-Seq Expression	MC11g0240.1
Synteny	MC11g0240.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTGCATTAGCAAGTCGTCATGTTTAGCCGTCAAAGAAATGCAAAATTCGGGCCTACAGAGCTCGAAGCCCATGGTGGTCTATGGGCCTAGCTTTCCTTCGGCCACCGCCAGGCCCATCATTTTGGGCCAGTTAAAGGAATCCAAAATTCGGGCCTTCACAAATTGGGCTGCGATAGTGTGAATACGATACATGTACAGGGTGAGAAAGCAATTTGGAAAAAGTTGGAGGGGCGTCTAACAAATTTGACGAAACTTTCCCAAAATTTGCGGCGGTCGTGTATTGGTCTTTACAAGCTATCTACTTGATCCCTACGCCCTTTCTTCTCCGTCCCTCCCTCTCTCTCACCGGTAATTCATCGCTCCATGGCTCTGCCTCAAAATCAGTACCAGCAACAGTATCAACCACAACATCAACACCAACAACAGCAATCTATTTCTTTCCGGTAATTCTCCTTCACCGCAATGTCAAAGGAGATTTTTTGTTATCGTTAAAACCCTAGCGATCGCCTCCTTCCTACAGTTTCGATTCTTTTCTGCATTCTTTTATTTTTGTATTGTCTCTCTAATTGCAGGAACTTGTACACTGCTGATGCTCATGTTTCTCAGCCGATCTCCTATTTCAACCCCTTCAATTTGCAGGATCATTCCCAGCATCCGCCCTACGTTCCTCCATGTAAATTCTCTTTTCCTTTATTTCTTTCATTCCTTTTACTTTGTGTGTTTCTTGGTTTCCCTGTCAGCTCATACTTTTGCTGAGAATTTGGGTCGAGACAATTCGAAGCAGTATATTTTTTGTTGGTTTGTTATTATGACAAAAAAAAAGCGCTATTTCTTACCAAACAGTTCATTTTTTTGTTCATGTTCTGGAATTTCCAAGGGTTACAAATTTAATAGAGACCATTTAAAGACTTTGGACTTAATTTTCGTTTTTCTACATCAACTGCCTCAGCAAACAAATGGAATCTCTCTGATTTAGCTCCTGTTTGTTCAAAATTCAATCTCAGCTTTTTAGTTGTTTGGACCAGTTTCATGTGCTTCTTATTTTAGACTAACATTTTGTTTACGGCATGTATTTTCTGCTATTCTAGTTCATGTAGTCGGGTTTGCACCAGGTACAGCCCCTGGACCGGATGCCAGTGATGGAGTAGCAGATTTTCCCTTGATGTATGGCATAGAACCCAAGAAGAAAAGATTAAAGGAGCAAGATCTTTTTGAAAACAATTCCCAGATATCATCCATTGATTTCTTGCAGCCACGTCCAGTTTCGACAGGACTGGGTTTATCACTTGACAACACCCGCATGGCTTCTACAGGAGACTCGCCTTTGGTTTCTCTTATAGGTGAAGACGTTGATCTCGAGTTACAGCGACAGGATGAAGATATTGAGAAGTTCCTCAAAGTTCAGGTCTGGATTACCATATTGTTTTGACAATTGATATATATAGTGTTTATTGAACTGGAAATATGGAAATCAGAGGGAGAGTAAAGGTTCTCAGACTATTTGAGAGGTCTGAGTCTCTCCTTCAACTAACTACCGAACGTGAAGGTCACATAGCTCCCTCTATACAAAAGATACCCTTCCTGGCACATGCTAATTGCTAACCTATCTTATTAATTCTTTATCATGAACTTGGTAGTGTTGTGGTTTATTAAATATATTTCTTGGTGTTTTGATGAAATAAAACAAGATGCAACAGGTCTATAATGAAGTTTGGTCTCATGTTCAAGCTTGCTTTTAATCCCGTTATTTTCACTTTTTGGATAGATACAGAATGTTGATTCCACTATATAATGAGCTTGGCTATCTATCATTCAATTTGCCTATGAATTAGTTGCTTGCCATTTTTTTAGACGTGAGTGTCTAAGCTGACTTACCTCAACTAATCCCACAAGATTATTTCTTTAACCCTGTACCATTTGATTTTGAAAAATTCATAGTTCAACCTATGACTTCAAAGACGTTGGAGTTTATAGCTTGTCCTTTTGACCATTGGATGTTGGCCAATCCTTCATGGTTGTCTTATCAGGGTTATCTTTGTTTCTGAATTTGGGTTTCAATTAGAGGCAAGTTAATACTGTTATGATACATGGTGCTTTCTTTTACCTTGTGGAAATAAGTTCCAGTGTGTTCCTTTTACATTGTTTTTATTGATAGATCTTTTCTTTGTAGAAAAGAAATATTGTTAGAAGTTAGAATTTTTATCTATCTATTTTCTCACAGCATTATTTTGTTTTAATAATTTGGCCGCATATCCAGTATGCACTCTCCTAACCTTTTCTTACTGTTGCTGGCTATTTATAAGTCAGTGTGGCACCTTGTTTCTTGTTTACTTAATAGTTCTCTTTGTGATCAGTTACCACAATGGCTTTAGTGAATTGTTGCATTGCATGTTTGATATTGGCCATTTTATTACATCAAGAATGTCTTCATAGCCGTTCCAATTATGGGTGGTTCTGAATTACAGTCATATACCTTCCTCCTCTAATCCCCTTTTTATCAATAGGGTGATAGGTTACGACATACCATATTAGAGAAGATCCAAGCCAATCAACTCCAGACTCTCTCATTTGTGGAGGAAAGAATTCTTCAGAAACTGCGTGAAAAAGAAGCTGAGGTGGAGTGTATTAACAAGAAAAACATTGAGCTCGAACAACGAATGGAGCAGCTGACTGTGGAAGCAGGTGCTTGGCAGCAGCGGGCTAGATATAACGAAAACATGATCACAGCCCTCAAGTTTAATCTTCAGCAAGTGTATGCCCAAAGCAGGGATAGCAAGGAAGGATGTGGGGATAGTGAGGTCGAAGACACTGCCTCGTGCTGCAATGGTCGGTCCCTTGATTTTCAACTGCTTTGCAGCAACAGCAATGACATGAAGGAGTTGATGACCTGTAAGGCTTGCAGGGTTAATGAAGTGTGTATGCTTTTGTTGCCTTGTAAGCATCTCTGCCTGTGCAAGGATTGCGAAAGTAAGCTTACCTTTTGCCCCTTGTGTCAATCCTCTAAATTCATAGGCATGGAAGTTTATATGTAGAGGGATGCTTTGTGTACGACTAAAGTATATGCTTAATTCGTATATGTTTCTCATGTGATAATGATGTCTTTGTTTCTGCTAAGTTGTTCCCTATGTTATACGCGTCTCATTTCATAGAGTTGTCATACGATTTATGACTTAACATCTTGAATTTGTATACTAACTATCTTTTATTTGTAGTTAATATCCAAACGTTTATAGAAATAATATAGACTTGTACGCTGGAGGTTTGTCTTTTGTCAATTTTTTTTTTCAAAAGTGAATGGATGTGTATTAACC

mRNA sequence

CTTGCATTAGCAAGTCGTCATGTTTAGCCGTCAAAGAAATGCAAAATTCGGGCCTACAGAGCTCGAAGCCCATGGTGGTCTATGGGCCTAGCTTTCCTTCGGCCACCGCCAGGCCCATCATTTTGGGCCAGTTAAAGGAATCCAAAATTCGGGCCTTCACAAATTGGGCTGCGATAGTGTGAATACGATACATGTACAGGGTGAGAAAGCAATTTGGAAAAAGTTGGAGGGGCGTCTAACAAATTTGACGAAACTTTCCCAAAATTTGCGGCGGTCGTGTATTGGTCTTTACAAGCTATCTACTTGATCCCTACGCCCTTTCTTCTCCGTCCCTCCCTCTCTCTCACCGGTAATTCATCGCTCCATGGCTCTGCCTCAAAATCAGTACCAGCAACAGTATCAACCACAACATCAACACCAACAACAGCAATCTATTTCTTTCCGGAACTTGTACACTGCTGATGCTCATGTTTCTCAGCCGATCTCCTATTTCAACCCCTTCAATTTGCAGGATCATTCCCAGCATCCGCCCTACGTTCCTCCATGTACAGCCCCTGGACCGGATGCCAGTGATGGAGTAGCAGATTTTCCCTTGATGTATGGCATAGAACCCAAGAAGAAAAGATTAAAGGAGCAAGATCTTTTTGAAAACAATTCCCAGATATCATCCATTGATTTCTTGCAGCCACGTCCAGTTTCGACAGGACTGGGTTTATCACTTGACAACACCCGCATGGCTTCTACAGGAGACTCGCCTTTGGTTTCTCTTATAGGTGAAGACGTTGATCTCGAGTTACAGCGACAGGATGAAGATATTGAGAAGTTCCTCAAAGTTCAGGGTGATAGGTTACGACATACCATATTAGAGAAGATCCAAGCCAATCAACTCCAGACTCTCTCATTTGTGGAGGAAAGAATTCTTCAGAAACTGCGTGAAAAAGAAGCTGAGGTGGAGTGTATTAACAAGAAAAACATTGAGCTCGAACAACGAATGGAGCAGCTGACTGTGGAAGCAGGTGCTTGGCAGCAGCGGGCTAGATATAACGAAAACATGATCACAGCCCTCAAGTTTAATCTTCAGCAAGTGTATGCCCAAAGCAGGGATAGCAAGGAAGGATGTGGGGATAGTGAGGTCGAAGACACTGCCTCGTGCTGCAATGGTCGGTCCCTTGATTTTCAACTGCTTTGCAGCAACAGCAATGACATGAAGGAGTTGATGACCTGTAAGGCTTGCAGGGTTAATGAAGTGTGTATGCTTTTGTTGCCTTGTAAGCATCTCTGCCTGTGCAAGGATTGCGAAAGTAAGCTTACCTTTTGCCCCTTGTGTCAATCCTCTAAATTCATAGGCATGGAAGTTTATATGTAGAGGGATGCTTTGTGTACGACTAAAGTATATGCTTAATTCGTATATGTTTCTCATGTGATAATGATGTCTTTGTTTCTGCTAAGTTGTTCCCTATGTTATACGCGTCTCATTTCATAGAGTTGTCATACGATTTATGACTTAACATCTTGAATTTGTATACTAACTATCTTTTATTTGTAGTTAATATCCAAACGTTTATAGAAATAATATAGACTTGTACGCTGGAGGTTTGTCTTTTGTCAATTTTTTTTTTCAAAAGTGAATGGATGTGTATTAACC

Coding sequence (CDS)

ATGGCTCTGCCTCAAAATCAGTACCAGCAACAGTATCAACCACAACATCAACACCAACAACAGCAATCTATTTCTTTCCGGAACTTGTACACTGCTGATGCTCATGTTTCTCAGCCGATCTCCTATTTCAACCCCTTCAATTTGCAGGATCATTCCCAGCATCCGCCCTACGTTCCTCCATGTACAGCCCCTGGACCGGATGCCAGTGATGGAGTAGCAGATTTTCCCTTGATGTATGGCATAGAACCCAAGAAGAAAAGATTAAAGGAGCAAGATCTTTTTGAAAACAATTCCCAGATATCATCCATTGATTTCTTGCAGCCACGTCCAGTTTCGACAGGACTGGGTTTATCACTTGACAACACCCGCATGGCTTCTACAGGAGACTCGCCTTTGGTTTCTCTTATAGGTGAAGACGTTGATCTCGAGTTACAGCGACAGGATGAAGATATTGAGAAGTTCCTCAAAGTTCAGGGTGATAGGTTACGACATACCATATTAGAGAAGATCCAAGCCAATCAACTCCAGACTCTCTCATTTGTGGAGGAAAGAATTCTTCAGAAACTGCGTGAAAAAGAAGCTGAGGTGGAGTGTATTAACAAGAAAAACATTGAGCTCGAACAACGAATGGAGCAGCTGACTGTGGAAGCAGGTGCTTGGCAGCAGCGGGCTAGATATAACGAAAACATGATCACAGCCCTCAAGTTTAATCTTCAGCAAGTGTATGCCCAAAGCAGGGATAGCAAGGAAGGATGTGGGGATAGTGAGGTCGAAGACACTGCCTCGTGCTGCAATGGTCGGTCCCTTGATTTTCAACTGCTTTGCAGCAACAGCAATGACATGAAGGAGTTGATGACCTGTAAGGCTTGCAGGGTTAATGAAGTGTGTATGCTTTTGTTGCCTTGTAAGCATCTCTGCCTGTGCAAGGATTGCGAAAGTAAGCTTACCTTTTGCCCCTTGTGTCAATCCTCTAAATTCATAGGCATGGAAGTTTATATGTAG

Protein sequence

MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPPCTAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLDNTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSFVEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMITALKFNLQQVYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLLPCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM

Homology

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BLAST of MC11g0240.1 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.8e-23
Identity = 76/244 (31.15%), Postives = 124/244 (50.82%), Query Frame = 0


Query: 117 LSLDNTRMAS-------TGDSPLVSLIGEDVDL--ELQRQDEDIEKFLKV---QGDRLRH 176
           L L N R +S       T  + L S +G+D+D+   + +Q  +I++F+ +   Q +R+++
Sbjct: 113 LLLQNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQHEIDRFVSLHLYQMERVKY 172

Query: 177 TILEKIQANQLQTLSFVEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRA 236
            I EK +      +  +E+ ++++LR KE E E I K N  LE+R++ L++E   W+  A
Sbjct: 173 EIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQIWRDLA 232

Query: 237 RYNENMITALKFNLQQVYAQSRDSKEGCG----DSEVEDTASCCNG-----------RSL 296
           + NE     L+ NL+ V AQ +D   G G     +E +D  SCC             R +
Sbjct: 233 QTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDDAESCCGSSCGGGGEETVRRRV 292

Query: 297 DFQLLCSNSNDMKELMTCKACRVNEVCMLLLPCKHLCLCKDCESKLTFCPLCQSSKFIGM 334
             +    +  + +    C+ C   E C+LLLPC+HLCLC  C S +  CP+C S K   +
Sbjct: 293 GLEREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCLCGVCGSSVHTCPICTSPKNASV 352

[Close]

BLAST of MC11g0240.1 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.2e-21
Identity = 72/246 (29.27%), Postives = 125/246 (50.81%), Query Frame = 0


Query: 101 SSIDFLQP---RPVSTGLGLSLDNTRMASTGDSPL--VSLIGEDVDLELQRQDEDIEKFL 160
           +++DFL+P   R  S    + L+ +       +P   +  +G+D+   +Q+   DI++ +
Sbjct: 86  NNVDFLRPVSSRKRSREESVVLNPSAYMQIQKNPTDPLMFLGQDLSSNVQQHHFDIDRLI 145

Query: 161 KVQGDRLRHTILEKIQANQLQTLSFVEERILQKLREKEAEVECINKKNIELEQRMEQLTV 220
               +R+R  I EK +    + +  VE+ +++ LR K+ E+  I K N+ LE++++ L V
Sbjct: 146 SNHVERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKDDEINHIGKLNLFLEEKVKSLCV 205

Query: 221 EAGAWQQRARYNENMITALKFNLQQVYAQSRDSKEGCGDSEVEDTASCCNGR-----SLD 280
           E   W+  A+ NE  + AL+ NLQQV A    ++     +  +D  SCC          +
Sbjct: 206 ENQIWRDVAQSNEATVNALRSNLQQVLAAVERNRWEEPPTVADDAQSCCGSNDEGDSEEE 265

Query: 281 FQLLCSNSNDMKE-----LMTCKACRVNEVCMLLLPCKHLCLCKDCESKLTFCPLCQSSK 332
              L   + D K+     +  C++C   E  +LLLPC+H+CLC  C S L  CP+C+S K
Sbjct: 266 RWKLAGEAQDTKKMCRVGMSMCRSCGKGEASVLLLPCRHMCLCSVCGSSLNTCPICKSPK 325

[Close]

BLAST of MC11g0240.1 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.2e-13
Identity = 62/216 (28.70%), Postives = 100/216 (46.30%), Query Frame = 0


Query: 134 SLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSFVEERILQKLREKE 193
           SLI  ++  ++Q+Q+ +I++F+  Q + LR  +  + +       S V+  IL+KL+ K+
Sbjct: 96  SLIDAELVSQIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKD 155

Query: 194 AEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMITALKFNLQQVYAQ--------- 253
            E+  + K N  L++R++ L VE   W+  A+ NE     L+ NL+QV AQ         
Sbjct: 156 EEIIRMGKLNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRR 215

Query: 254 -----SRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLL 313
                + D++  CG  +  D  +  NG                    CK C      +L+
Sbjct: 216 PLVEEADDAESSCGSCDGGDVTAVVNG-------------------GCKRCGELTASVLV 275

Query: 314 LPCKHLCLCKDCESK--LTFCPLCQSSKFIGMEVYM 334
           LPC+HLCLC  C S   L  CP+C       + V M
Sbjct: 276 LPCRHLCLCTVCGSSALLRTCPVCDMVMTASVHVNM 292

[Close]

BLAST of MC11g0240.1 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 5.8e-13
Identity = 65/201 (32.34%), Postives = 96/201 (47.76%), Query Frame = 0


Query: 129 DSPLVSLIGEDVDLELQRQDE-DIEKFLKVQGDRLRHTILEKIQANQLQTLSFVEERILQ 188
           DS   SLI  ++  ++Q Q + +I++F+  Q ++LR  I  + Q       S V+  I +
Sbjct: 100 DSSSPSLINVELVSQIQNQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAK 159

Query: 189 KLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMITALKFNLQQVYAQSRD 248
           KL+EK+ E+  I   N  L++R++ L VE   W+  A+ NE     L+ NL QV AQ   
Sbjct: 160 KLKEKDDEIVRIRNLNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQLET 219

Query: 249 SKEGCGDSEVEDTA-----SCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLLPC 308
                  + VED A     SCC     +                CK C   E  +L+LPC
Sbjct: 220 FP--TASAVVEDDAESSCGSCCGDGGGEAVTAVGGG--------CKRCGEREASVLVLPC 279

Query: 309 KHLCLCKDC--ESKLTFCPLC 322
           +HLCLC  C   + L  CP+C
Sbjct: 280 RHLCLCTVCGGSALLRTCPVC 290

[Close]

BLAST of MC11g0240.1 vs. ExPASy Swiss-Prot
Match: Q13489 (Baculoviral IAP repeat-containing protein 3 OS=Homo sapiens OX=9606 GN=BIRC3 PE=1 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 7.5e-05
Identity = 20/44 (45.45%), Postives = 29/44 (65.91%), Query Frame = 0


Query: 281 MKELMTCKACRVNEVCMLLLPCKHLCLCKDCESKLTFCPLCQSS 325
           ++E  TCK C   EV ++ +PC HL +CKDC   L  CP+C+S+
Sbjct: 551 LQEERTCKVCMDKEVSIVFIPCGHLVVCKDCAPSLRKCPICRST 594

[Close]

BLAST of MC11g0240.1 vs. NCBI nr
Match: XP_022139921.1 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Momordica charantia])

HSP 1 Score: 661 bits (1706), Expect = 1.63e-239
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0


Query: 1   MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP 60
           MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP
Sbjct: 1   MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP 60

Query: 61  CTAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD 120
           CTAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD
Sbjct: 61  CTAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD 120

Query: 121 NTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSF 180
           NTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSF
Sbjct: 121 NTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSF 180

Query: 181 VEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMITALKFNLQQ 240
           VEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMITALKFNLQQ
Sbjct: 181 VEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMITALKFNLQQ 240

Query: 241 VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLL 300
           VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLL
Sbjct: 241 VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLL 300

Query: 301 PCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM 333
           PCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM
Sbjct: 301 PCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM 333

[Close]

BLAST of MC11g0240.1 vs. NCBI nr
Match: XP_022139913.1 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Momordica charantia])

HSP 1 Score: 650 bits (1676), Expect = 8.23e-235
Identity = 332/341 (97.36%), Postives = 332/341 (97.36%), Query Frame = 0


Query: 1   MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP 60
           MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP
Sbjct: 1   MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP 60

Query: 61  C--------TAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVS 120
                    TAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVS
Sbjct: 61  FHVVGFAPGTAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVS 120

Query: 121 TGLGLSLDNTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQA 180
           TGLGLSLDNTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQA
Sbjct: 121 TGLGLSLDNTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQA 180

Query: 181 NQLQTLSFVEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMIT 240
           NQLQTLSFVEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMIT
Sbjct: 181 NQLQTLSFVEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMIT 240

Query: 241 ALKFNLQQVYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRV 300
           ALKFNLQQVYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRV
Sbjct: 241 ALKFNLQQVYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRV 300

Query: 301 NEVCMLLLPCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM 333
           NEVCMLLLPCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM
Sbjct: 301 NEVCMLLLPCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM 341

[Close]

BLAST of MC11g0240.1 vs. NCBI nr
Match: XP_023535989.1 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 603 bits (1555), Expect = 1.51e-216
Identity = 306/333 (91.89%), Postives = 317/333 (95.20%), Query Frame = 0


Query: 1   MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP 60
           MALPQN YQQ YQ Q QH   QSISFRNLYTAD HVSQPISYFNPFNLQ+HSQHPPYVPP
Sbjct: 1   MALPQNHYQQHYQSQQQH---QSISFRNLYTADGHVSQPISYFNPFNLQEHSQHPPYVPP 60

Query: 61  CTAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD 120
           CTAPG DASDGVADF  M+GIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD
Sbjct: 61  CTAPGQDASDGVADFHWMHGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD 120

Query: 121 NTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSF 180
           NTRMASTGDSPLVSLIG+D+DLELQRQDEDIEKFL VQG+RLRHTILEKIQANQLQTLS 
Sbjct: 121 NTRMASTGDSPLVSLIGDDIDLELQRQDEDIEKFLHVQGERLRHTILEKIQANQLQTLST 180

Query: 181 VEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMITALKFNLQQ 240
           VEERI++KLREKEAEVECINKKNIELEQRMEQL+VEAGAWQQRARYNENMITALKFNLQQ
Sbjct: 181 VEERIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQ 240

Query: 241 VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLL 300
           VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLL  NSNDMK+LM CKACRVNEVCMLLL
Sbjct: 241 VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLRGNSNDMKDLMYCKACRVNEVCMLLL 300

Query: 301 PCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM 333
           PCKHLCLCKDCES+L+FCPLCQSSKFIGMEVYM
Sbjct: 301 PCKHLCLCKDCESRLSFCPLCQSSKFIGMEVYM 330

[Close]

BLAST of MC11g0240.1 vs. NCBI nr
Match: KAG6591387.1 (putative BOI-related E3 ubiquitin-protein ligase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 602 bits (1552), Expect = 4.32e-216
Identity = 305/333 (91.59%), Postives = 317/333 (95.20%), Query Frame = 0


Query: 1   MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP 60
           MALPQN YQQ YQ Q QH   QSISFRNLYTAD HVSQPISYFNPFNLQ+HSQHPPYVPP
Sbjct: 1   MALPQNHYQQHYQSQQQH---QSISFRNLYTADGHVSQPISYFNPFNLQEHSQHPPYVPP 60

Query: 61  CTAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD 120
           CTAPG DASDGVADF  M+GIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD
Sbjct: 61  CTAPGQDASDGVADFHWMHGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD 120

Query: 121 NTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSF 180
           NTRMASTGDSPLVSLIG+D+DLELQRQDEDIEKFL VQG+RLRHTILEKIQANQLQTLS 
Sbjct: 121 NTRMASTGDSPLVSLIGDDIDLELQRQDEDIEKFLHVQGERLRHTILEKIQANQLQTLST 180

Query: 181 VEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMITALKFNLQQ 240
           VEERI++KLREKEAEVECINKKNIELEQRMEQL++EAGAWQQRARYNENMITALKFNLQQ
Sbjct: 181 VEERIIKKLREKEAEVECINKKNIELEQRMEQLSMEAGAWQQRARYNENMITALKFNLQQ 240

Query: 241 VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLL 300
           VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLL  NSNDMK+LM CKACRVNEVCMLLL
Sbjct: 241 VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLRGNSNDMKDLMYCKACRVNEVCMLLL 300

Query: 301 PCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM 333
           PCKHLCLCKDCES+L+FCPLCQSSKFIGMEVYM
Sbjct: 301 PCKHLCLCKDCESRLSFCPLCQSSKFIGMEVYM 330

[Close]

BLAST of MC11g0240.1 vs. NCBI nr
Match: XP_022937285.1 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 600 bits (1548), Expect = 1.76e-215
Identity = 304/333 (91.29%), Postives = 317/333 (95.20%), Query Frame = 0


Query: 1   MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP 60
           MALPQN YQQ YQ Q QH   QSISFRNLYTAD HVSQPISYFNPFNLQ+HSQHPPYVPP
Sbjct: 1   MALPQNHYQQHYQSQQQH---QSISFRNLYTADGHVSQPISYFNPFNLQEHSQHPPYVPP 60

Query: 61  CTAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD 120
           CTAPG DASDGVADF  M+GIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD
Sbjct: 61  CTAPGQDASDGVADFHWMHGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD 120

Query: 121 NTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSF 180
           NTRMASTGDSPLVSLIG+D+DLELQRQDEDIEKFL VQG+RLRHTILEKIQANQLQTLS 
Sbjct: 121 NTRMASTGDSPLVSLIGDDIDLELQRQDEDIEKFLHVQGERLRHTILEKIQANQLQTLST 180

Query: 181 VEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMITALKFNLQQ 240
           VEERI++KLREKEAEVECINKKNIELEQRMEQL++EAGAWQQRARYNENMITALKFNLQQ
Sbjct: 181 VEERIIKKLREKEAEVECINKKNIELEQRMEQLSMEAGAWQQRARYNENMITALKFNLQQ 240

Query: 241 VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLL 300
           VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLL  NSND+K+LM CKACRVNEVCMLLL
Sbjct: 241 VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLRGNSNDIKDLMYCKACRVNEVCMLLL 300

Query: 301 PCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM 333
           PCKHLCLCKDCES+L+FCPLCQSSKFIGMEVYM
Sbjct: 301 PCKHLCLCKDCESRLSFCPLCQSSKFIGMEVYM 330

[Close]

BLAST of MC11g0240.1 vs. ExPASy TrEMBL
Match: A0A6J1CE48 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010710 PE=4 SV=1)

HSP 1 Score: 661 bits (1706), Expect = 7.89e-240
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0


Query: 1   MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP 60
           MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP
Sbjct: 1   MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP 60

Query: 61  CTAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD 120
           CTAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD
Sbjct: 61  CTAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD 120

Query: 121 NTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSF 180
           NTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSF
Sbjct: 121 NTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSF 180

Query: 181 VEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMITALKFNLQQ 240
           VEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMITALKFNLQQ
Sbjct: 181 VEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMITALKFNLQQ 240

Query: 241 VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLL 300
           VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLL
Sbjct: 241 VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLL 300

Query: 301 PCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM 333
           PCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM
Sbjct: 301 PCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM 333

[Close]

BLAST of MC11g0240.1 vs. ExPASy TrEMBL
Match: A0A6J1CGS8 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010710 PE=4 SV=1)

HSP 1 Score: 650 bits (1676), Expect = 3.98e-235
Identity = 332/341 (97.36%), Postives = 332/341 (97.36%), Query Frame = 0


Query: 1   MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP 60
           MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP
Sbjct: 1   MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP 60

Query: 61  C--------TAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVS 120
                    TAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVS
Sbjct: 61  FHVVGFAPGTAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVS 120

Query: 121 TGLGLSLDNTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQA 180
           TGLGLSLDNTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQA
Sbjct: 121 TGLGLSLDNTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQA 180

Query: 181 NQLQTLSFVEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMIT 240
           NQLQTLSFVEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMIT
Sbjct: 181 NQLQTLSFVEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMIT 240

Query: 241 ALKFNLQQVYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRV 300
           ALKFNLQQVYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRV
Sbjct: 241 ALKFNLQQVYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRV 300

Query: 301 NEVCMLLLPCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM 333
           NEVCMLLLPCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM
Sbjct: 301 NEVCMLLLPCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM 341

[Close]

BLAST of MC11g0240.1 vs. ExPASy TrEMBL
Match: A0A6J1FG60 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443616 PE=4 SV=1)

HSP 1 Score: 600 bits (1548), Expect = 8.51e-216
Identity = 304/333 (91.29%), Postives = 317/333 (95.20%), Query Frame = 0


Query: 1   MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP 60
           MALPQN YQQ YQ Q QH   QSISFRNLYTAD HVSQPISYFNPFNLQ+HSQHPPYVPP
Sbjct: 1   MALPQNHYQQHYQSQQQH---QSISFRNLYTADGHVSQPISYFNPFNLQEHSQHPPYVPP 60

Query: 61  CTAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD 120
           CTAPG DASDGVADF  M+GIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD
Sbjct: 61  CTAPGQDASDGVADFHWMHGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD 120

Query: 121 NTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSF 180
           NTRMASTGDSPLVSLIG+D+DLELQRQDEDIEKFL VQG+RLRHTILEKIQANQLQTLS 
Sbjct: 121 NTRMASTGDSPLVSLIGDDIDLELQRQDEDIEKFLHVQGERLRHTILEKIQANQLQTLST 180

Query: 181 VEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMITALKFNLQQ 240
           VEERI++KLREKEAEVECINKKNIELEQRMEQL++EAGAWQQRARYNENMITALKFNLQQ
Sbjct: 181 VEERIIKKLREKEAEVECINKKNIELEQRMEQLSMEAGAWQQRARYNENMITALKFNLQQ 240

Query: 241 VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLL 300
           VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLL  NSND+K+LM CKACRVNEVCMLLL
Sbjct: 241 VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLRGNSNDIKDLMYCKACRVNEVCMLLL 300

Query: 301 PCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM 333
           PCKHLCLCKDCES+L+FCPLCQSSKFIGMEVYM
Sbjct: 301 PCKHLCLCKDCESRLSFCPLCQSSKFIGMEVYM 330

[Close]

BLAST of MC11g0240.1 vs. ExPASy TrEMBL
Match: A0A6J1IEH6 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476508 PE=4 SV=1)

HSP 1 Score: 596 bits (1537), Expect = 4.04e-214
Identity = 303/333 (90.99%), Postives = 315/333 (94.59%), Query Frame = 0


Query: 1   MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP 60
           MALPQN YQQ YQ Q QH   QSISFRNLYTA+ HVSQPISYFNPFNLQ+HSQHPPYVPP
Sbjct: 1   MALPQNHYQQHYQSQQQH---QSISFRNLYTAEGHVSQPISYFNPFNLQEHSQHPPYVPP 60

Query: 61  CTAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD 120
           CTAPG DASDGVADF  M+GIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD
Sbjct: 61  CTAPGQDASDGVADFHWMHGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD 120

Query: 121 NTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSF 180
           NTRMASTGDSPLVSLIG+D+DLELQRQDEDIEKFL VQG+RLR TILEKIQANQLQTL  
Sbjct: 121 NTRMASTGDSPLVSLIGDDIDLELQRQDEDIEKFLHVQGERLRRTILEKIQANQLQTLLT 180

Query: 181 VEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMITALKFNLQQ 240
           VEERI++KLREKEAEVECINKKNIELEQRMEQL+VEAGAWQQRARYNENMITALKFNLQQ
Sbjct: 181 VEERIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQ 240

Query: 241 VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLL 300
           VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLL  NSNDMK+LM CKACRVNEVCMLLL
Sbjct: 241 VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLRGNSNDMKDLMYCKACRVNEVCMLLL 300

Query: 301 PCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM 333
           PCKHLCLCKDCES+L+FCPLCQSSKFIGMEVYM
Sbjct: 301 PCKHLCLCKDCESRLSFCPLCQSSKFIGMEVYM 330

[Close]

BLAST of MC11g0240.1 vs. ExPASy TrEMBL
Match: A0A5A7VDE1 (Putative BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003250 PE=4 SV=1)

HSP 1 Score: 596 bits (1536), Expect = 6.18e-214
Identity = 301/333 (90.39%), Postives = 316/333 (94.89%), Query Frame = 0


Query: 1   MALPQNQYQQQYQPQHQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPP 60
           MALPQN YQQ YQ QHQ QQ QSISFRNLYTAD HVSQPISYFN FNLQDHSQHPPYVPP
Sbjct: 1   MALPQNHYQQHYQSQHQ-QQHQSISFRNLYTADGHVSQPISYFNSFNLQDHSQHPPYVPP 60

Query: 61  CTAPGPDASDGVADFPLMYGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLD 120
           CTAPG DASDGVAD   MYGIEPKKKRLKEQDLFENNSQISSIDFLQ RPVSTGLGLSLD
Sbjct: 61  CTAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLD 120

Query: 121 NTRMASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSF 180
           NTRMASTGDSPLVSL+G+D+D ELQ+QDE+IEKFLKVQGDRLRH+ILEKIQANQLQTLS 
Sbjct: 121 NTRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSI 180

Query: 181 VEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMITALKFNLQQ 240
           VEE+I++KLREKEAEVECINKKNIELEQRMEQL+VEAGAWQQRARYNENMITALKFNLQQ
Sbjct: 181 VEEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQ 240

Query: 241 VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLL 300
           VYAQSRDSKEGCGDSEVEDTASCCNGR+LDFQLL SNSND+KELM CKACRVNEVCMLL 
Sbjct: 241 VYAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLRSNSNDVKELMYCKACRVNEVCMLLF 300

Query: 301 PCKHLCLCKDCESKLTFCPLCQSSKFIGMEVYM 333
           PCKHLCLCKDCES+LTFCP+CQSSKF+GMEVYM
Sbjct: 301 PCKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 332

[Close]

BLAST of MC11g0240.1 vs. TAIR 10
Match: AT1G45976.1 (S-ribonuclease binding protein 1 )

HSP 1 Score: 365.9 bits (938), Expect = 3.4e-101
Identity = 196/330 (59.39%), Postives = 245/330 (74.24%), Query Frame = 0


Query: 16  HQHQQQQSISFRNLYTADAHVSQPISYFNPFNLQDHSQHPPYVPPC----TAPGP----D 75
           H H  Q S +FR+    D  +S  + +    NL D SQHPPY+PP      APGP    D
Sbjct: 4   HPH-LQDSKNFRDFCGIDGQISPELGFNRSENLHDQSQHPPYIPPFHVAGFAPGPVVQID 63

Query: 76  ASDG--VADFPLMY--GIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLDNTR 135
            SDG   ADF   Y  G+EP+++R+KEQD  ENNSQISSIDFLQ R VSTGLGLSLDN R
Sbjct: 64  GSDGGNGADFEWNYGLGLEPRRERIKEQDFLENNSQISSIDFLQARSVSTGLGLSLDNAR 123

Query: 136 MASTGDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSFVEE 195
           +AS+  S L+SL+G+D+D ELQRQD DI++FLK+QGD+LRH IL+KI+  Q +T+S +EE
Sbjct: 124 VASSDGSALLSLVGDDIDRELQRQDADIDRFLKIQGDQLRHAILDKIKRGQQKTVSLMEE 183

Query: 196 RILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQQRARYNENMITALKFNLQQVYA 255
           +++QKLREK+ E+E IN+KN ELE RMEQLT+EA AWQQRA+YNENMI AL +NL +   
Sbjct: 184 KVVQKLREKDEELERINRKNKELEVRMEQLTMEAEAWQQRAKYNENMIAALNYNLDRAQG 243

Query: 256 QSRDSKEGCGDSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLLPCK 315
           + RDS EGCGDSEV+DTASC NGR        +++N+ K +M C+ C V E+CMLLLPC 
Sbjct: 244 RPRDSIEGCGDSEVDDTASCFNGRD-------NSNNNTKTMMMCRFCGVREMCMLLLPCN 303

Query: 316 HLCLCKDCESKLTFCPLCQSSKFIGMEVYM 334
           H+CLCK+CE KL+ CPLCQSSKF+GMEVYM
Sbjct: 304 HMCLCKECERKLSSCPLCQSSKFLGMEVYM 325

[Close]

BLAST of MC11g0240.1 vs. TAIR 10
Match: AT1G60610.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 146.7 bits (369), Expect = 3.2e-35
Identity = 90/259 (34.75%), Postives = 150/259 (57.92%), Query Frame = 0


Query: 94  FENNSQISSIDFLQPRPVSTGLGLSLD----NTRMASTGDS---PLVSLIGEDVDLELQR 153
           + NN+ +   +  +   VSTGL LS D    N+ + S   S   P+   +G+++ L+L R
Sbjct: 88  YNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNSSVTSANGSITTPVYQSLGDNIRLDLNR 147

Query: 154 QDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSFVEERILQKLREKEAEVECINKKNIEL 213
           Q++++++F+K + D++   + +  Q +    ++ +E+ + +KL+EK+ E+E +NKKN EL
Sbjct: 148 QNDELDQFIKFRADQMAKGVRDIKQRHVTSFVTALEKDVSKKLQEKDHEIESMNKKNREL 207

Query: 214 EQRMEQLTVEAGAWQQRARYNENMITALKFNLQQVYAQSRDS-------------KEGCG 273
             +++Q+ VEA  W  +A+YNE+++ ALK NLQQV +   D+             KEG G
Sbjct: 208 VDKIKQVAVEAQNWHYKAKYNESVVNALKVNLQQVMSHGNDNNAVGGGVADHHQMKEGFG 267

Query: 274 DSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLLPCKHLCLCKDCES 333
           DSE++D A+  N   L+   + S        M CK C V  V +LL+PC+HL LCKDC+ 
Sbjct: 268 DSEIDDEAASYN--YLNIPGMPSTG------MRCKLCNVKNVSVLLVPCRHLSLCKDCDV 327

[Close]

BLAST of MC11g0240.1 vs. TAIR 10
Match: AT1G60610.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 146.7 bits (369), Expect = 3.2e-35
Identity = 90/259 (34.75%), Postives = 150/259 (57.92%), Query Frame = 0


Query: 94  FENNSQISSIDFLQPRPVSTGLGLSLD----NTRMASTGDS---PLVSLIGEDVDLELQR 153
           + NN+ +   +  +   VSTGL LS D    N+ + S   S   P+   +G+++ L+L R
Sbjct: 88  YNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNSSVTSANGSITTPVYQSLGDNIRLDLNR 147

Query: 154 QDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSFVEERILQKLREKEAEVECINKKNIEL 213
           Q++++++F+K + D++   + +  Q +    ++ +E+ + +KL+EK+ E+E +NKKN EL
Sbjct: 148 QNDELDQFIKFRADQMAKGVRDIKQRHVTSFVTALEKDVSKKLQEKDHEIESMNKKNREL 207

Query: 214 EQRMEQLTVEAGAWQQRARYNENMITALKFNLQQVYAQSRDS-------------KEGCG 273
             +++Q+ VEA  W  +A+YNE+++ ALK NLQQV +   D+             KEG G
Sbjct: 208 VDKIKQVAVEAQNWHYKAKYNESVVNALKVNLQQVMSHGNDNNAVGGGVADHHQMKEGFG 267

Query: 274 DSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLLPCKHLCLCKDCES 333
           DSE++D A+  N   L+   + S        M CK C V  V +LL+PC+HL LCKDC+ 
Sbjct: 268 DSEIDDEAASYN--YLNIPGMPSTG------MRCKLCNVKNVSVLLVPCRHLSLCKDCDV 327

[Close]

BLAST of MC11g0240.1 vs. TAIR 10
Match: AT1G60610.3 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 146.7 bits (369), Expect = 3.2e-35
Identity = 90/259 (34.75%), Postives = 150/259 (57.92%), Query Frame = 0


Query: 94  FENNSQISSIDFLQPRPVSTGLGLSLD----NTRMASTGDS---PLVSLIGEDVDLELQR 153
           + NN+ +   +  +   VSTGL LS D    N+ + S   S   P+   +G+++ L+L R
Sbjct: 88  YNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNSSVTSANGSITTPVYQSLGDNIRLDLNR 147

Query: 154 QDEDIEKFLKVQGDRLRHTILEKIQANQLQTLSFVEERILQKLREKEAEVECINKKNIEL 213
           Q++++++F+K + D++   + +  Q +    ++ +E+ + +KL+EK+ E+E +NKKN EL
Sbjct: 148 QNDELDQFIKFRADQMAKGVRDIKQRHVTSFVTALEKDVSKKLQEKDHEIESMNKKNREL 207

Query: 214 EQRMEQLTVEAGAWQQRARYNENMITALKFNLQQVYAQSRDS-------------KEGCG 273
             +++Q+ VEA  W  +A+YNE+++ ALK NLQQV +   D+             KEG G
Sbjct: 208 VDKIKQVAVEAQNWHYKAKYNESVVNALKVNLQQVMSHGNDNNAVGGGVADHHQMKEGFG 267

Query: 274 DSEVEDTASCCNGRSLDFQLLCSNSNDMKELMTCKACRVNEVCMLLLPCKHLCLCKDCES 333
           DSE++D A+  N   L+   + S        M CK C V  V +LL+PC+HL LCKDC+ 
Sbjct: 268 DSEIDDEAASYN--YLNIPGMPSTG------MRCKLCNVKNVSVLLVPCRHLSLCKDCDV 327

[Close]

BLAST of MC11g0240.1 vs. TAIR 10
Match: AT1G10650.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 138.7 bits (348), Expect = 8.8e-33
Identity = 83/238 (34.87%), Postives = 139/238 (58.40%), Query Frame = 0


Query: 111 VSTGLGLSLDNTRMAST---------GDSPLVSLIGEDVDLELQRQDEDIEKFLKVQGDR 170
           VSTGL LS D+    S+           SP+   + + + ++L RQ ++ ++F+K+Q  +
Sbjct: 104 VSTGLRLSYDDDEHNSSVTSASGSILAASPIFQSLDDSLRIDLHRQKDEFDQFIKIQAAQ 163

Query: 171 LRHTILEKIQANQLQTLSFVEERILQKLREKEAEVECINKKNIELEQRMEQLTVEAGAWQ 230
           +   + +  Q +    L+ +E+ + +KL+EK+ E+  +NKKN EL +R++Q+ +EA  W 
Sbjct: 164 MAKGVRDMKQRHIASFLTTLEKGVSKKLQEKDHEINDMNKKNKELVERIKQVAMEAQNWH 223

Query: 231 QRARYNENMITALKFNLQQ-------VYAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLL 290
            RA+YNE+++  LK NLQQ       V A +   KEG GDSE++D AS      +D    
Sbjct: 224 YRAKYNESVVNVLKANLQQAMSHNNSVIAAADQGKEGFGDSEIDDAAS----SYIDPNNN 283

Query: 291 CSNSNDMKELMTCKACRVNEVCMLLLPCKHLCLCKDCESKLTFCPLCQSSKFIGMEVY 333
            +N+  + + M CK C V EV +L++PC+HL LCK+C+     CP+C+S K   ++V+
Sbjct: 284 NNNNMGIHQRMRCKMCNVKEVSVLIVPCRHLSLCKECDVFTKICPVCKSLKSSCVQVF 337

The following BLAST results are available for this feature:

BLAST of MC11g0240.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4IDI6	2.8e-23	31.15	Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37...	[more]
Q9LDD1	1.2e-21	29.27	Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37...	[more]
Q9FHE4	1.2e-13	28.70	BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG...	[more]
O81851	5.8e-13	32.34	E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1	[more]
Q13489	7.5e-05	45.45	Baculoviral IAP repeat-containing protein 3 OS=Homo sapiens OX=9606 GN=BIRC3 PE=...	[more]

BLAST of MC11g0240.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139921.1	1.63e-239	100.00	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Momordica charant...	[more]
XP_022139913.1	8.23e-235	97.36	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Momordica charant...	[more]
XP_023535989.1	1.51e-216	91.89	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucurbita pepo su...	[more]
KAG6591387.1	4.32e-216	91.59	putative BOI-related E3 ubiquitin-protein ligase 3, partial [Cucurbita argyrospe...	[more]
XP_022937285.1	1.76e-215	91.29	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucurbita moschat...	[more]

BLAST of MC11g0240.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CE48	7.89e-240	100.00	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 OS=Momordica chara...	[more]
A0A6J1CGS8	3.98e-235	97.36	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Momordica chara...	[more]
A0A6J1FG60	8.51e-216	91.29	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita mosch...	[more]
A0A6J1IEH6	4.04e-214	90.99	probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita maxim...	[more]
A0A5A7VDE1	6.18e-214	90.39	Putative BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucumis melo va...	[more]

BLAST of MC11g0240.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G45976.1	3.4e-101	59.39	S-ribonuclease binding protein 1	[more]
AT1G60610.1	3.2e-35	34.75	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G60610.2	3.2e-35	34.75	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G60610.3	3.2e-35	34.75	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G10650.1	8.8e-33	34.87	SBP (S-ribonuclease binding protein) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 182..223
None	No IPR available	GENE3D	1.10.8.10		coord: 143..160 e-value: 1.2E-9 score: 40.1
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 286..323 e-value: 5.4E-7 score: 29.4
None	No IPR available	GENE3D	1.10.1170.10		coord: 296..323 e-value: 1.2E-9 score: 40.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	PANTHER	PTHR42647:SF10	F2G19.2	coord: 1..333
IPR017066	E3 ubiquitin-protein ligase BOI-like	PIRSF	PIRSF036836	SBP1_RNase_bind	coord: 1..333 e-value: 2.6E-87 score: 291.6
IPR017066	E3 ubiquitin-protein ligase BOI-like	PANTHER	PTHR42647	SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN	coord: 1..333
IPR011029	Death-like domain superfamily	GENE3D	1.10.533.10	Death Domain, Fas	coord: 161..295 e-value: 1.2E-9 score: 40.1
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 287..322 score: 9.243618

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC11g0240	MC11g0240	gene

The following utr5 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g0240.1-utr5	MC11g0240.1-utr5-MC11:1906680..1907043	utr5

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g0240.1-cds	MC11g0240.1-cds-MC11:1907044..1907123	CDS
MC11g0240.1-cds	MC11g0240.1-cds-MC11:1907253..1907353	CDS
MC11g0240.1-cds	MC11g0240.1-cds-MC11:1907793..1908085	CDS
MC11g0240.1-cds	MC11g0240.1-cds-MC11:1909185..1909712	CDS

The following utr3 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g0240.1-utr3	MC11g0240.1-utr3-MC11:1909713..1909991	utr3

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC11g0240.1	MC11g0240.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity