MC11g0055.1 (mRNA) Bitter gourd (Dali-11) v1

BLAST of MC11g0055.1 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 2.5e-226
Identity = 367/554 (66.25%), Postives = 451/554 (81.41%), Query Frame = 0

Query: 3   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 62
           MEG  +  AN V LTPI+FL+RSA VY DR S+VYG V+YTWR T  RC R+ASAL + G
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 63  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 122
           IS GDVV+ LAPN+PAM ELHF VPMAGA+LCTLN+RHD+++V+ LL HS  K+IF D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 123 FLPMVKRAIKLMSKTSEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFKI 182
           FL + + A +++S   +K+P++V+I E    +  ++    + E +E+E ++A G+ DF++
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPE--PLTQSVSRKKRSEEMMEYEDVVAMGKSDFEV 182

Query: 183 RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFHC 242
            RP DE D IS+NYTSGTTS PKGV+YSHRGAYLN+L+ VLLN+M S P YLWT PMFHC
Sbjct: 183 IRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHC 242

Query: 243 NGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKPL 302
           NGWC  WGV A GGTNIC R VTAK IF NISQH VTHMGGAPT+LNMIINAP SEQKPL
Sbjct: 243 NGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPL 302

Query: 303 PGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQAK 362
           PGKV+ +TG APPP+HV++KM  LGF + HSYGLTETYGP T+C+WKPEWDSLP ++QAK
Sbjct: 303 PGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAK 362

Query: 363 LNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFNG 422
           + +RQG+ H+GLE +++KDPVTM + PADG TMGEV+FRGNTVM+GYLK+ +AT+EAF G
Sbjct: 363 MKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKG 422

Query: 423 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPDD 482
           GWF SGDLGV+HPDGYIELKDRSKDIIISGGENISSIEVES L++HP +LE A+V RPD+
Sbjct: 423 GWFWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDE 482

Query: 483 HWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLKE 542
           +WGET CAFVKLKDG  A+ EE+I +CR+ LPHYMAPRS+VF DLPKTSTGK+QKFVL+ 
Sbjct: 483 YWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRT 542

Query: 543 EATAMGSLQRSKHS 557
           +A A+ SL +   S
Sbjct: 543 KAKALVSLSKKGRS 554

BLAST of MC11g0055.1 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 2.4e-213
Identity = 354/558 (63.44%), Postives = 439/558 (78.67%), Query Frame = 0

Query: 1   KSMEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVR 60
           +++EG+ R  AN   L+PITFLERSA VY DR SLV+G V++TW  T QRC RLASAL  
Sbjct: 46  RTIEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTN 105

Query: 61  KGISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVD 120
            GISRGDVVAALAPN+PAM+ELHFAVPMAG +LC LN R D + +S LL+HSEAKI+FVD
Sbjct: 106 LGISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVD 165

Query: 121 YQFLPMVKRAIKLMS---KTSEKLPLVVVIQEYDQPSSCINGVDSALE----DLEFESLL 180
           +Q L +   A+ L++   KT + L LV++ Q  D   S  +   +       D E+E+LL
Sbjct: 166 HQLLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLL 225

Query: 181 ATGEVDFKIRRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVY 240
            +G+ +F+I +P+ EWDPIS+NYTSGTTSRPKGV+YSHRGAYLN+L+TV L+ M   PVY
Sbjct: 226 KSGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVY 285

Query: 241 LWTVPMFHCNGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIIN 300
           LWTVPMFHCNGWC  WGVAAQGGTNIC R V+ K IF NI+ H VTHMGGAPTVLNMI+N
Sbjct: 286 LWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVN 345

Query: 301 APISEQKPLPGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWD 360
             ++E KPLP +V +MTGG+PP   +L KM  LGF + H YGLTETYGP T C WKPEWD
Sbjct: 346 YTVTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWD 405

Query: 361 SLPEDQQAKLNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDL 420
           SL  +++ KL +RQG+QH+GLEG+++KDP+TME+ P DG TMGEVMFRGNTVMSGY KD+
Sbjct: 406 SLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDI 465

Query: 421 KATQEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILE 480
           +AT++AF G WF SGDL V++PDGYIE+KDR KD+IISGGENISS+EVE VL SH ++LE
Sbjct: 466 EATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLE 525

Query: 481 VAIVGRPDDHWGETPCAFVKLKDGC-SANGEEIIKFCREHLPHYMAPRSVVFRDLPKTST 540
            A+V RPD HWG+TPC FVKLK+G  +   EEII FCR+HLPHYMAP+++VF D+PKTST
Sbjct: 526 AAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTST 585

Query: 541 GKIQKFVLKEEATAMGSL 551
           GK+QK++L+++A  MGSL
Sbjct: 586 GKVQKYLLRKKADEMGSL 603

BLAST of MC11g0055.1 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 2.4e-205
Identity = 349/554 (63.00%), Postives = 426/554 (76.90%), Query Frame = 0

Query: 1   KSMEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASAL-V 60
           KSMEG+ +CSAN V L+PITFLERS+  Y D  SLVYG VRYTW  T  RC +LASAL  
Sbjct: 27  KSMEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTT 86

Query: 61  RKGISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFV 120
             GIS GDVVA  + N+P +YELHFAVPMAG +LCTLN R+D+AMVSTLL+HSEAK+IFV
Sbjct: 87  HLGISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFV 146

Query: 121 DYQFLPMVKRAIKLMSKTSEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVD 180
           + Q L   + A+ L+++   K P +V++   D  S   +  D       +  LLA G  D
Sbjct: 147 EPQLLETARAALDLLAQKDIKPPTLVLLT--DSESFTSSSYD------HYNHLLANGSDD 206

Query: 181 FKIRRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPM 240
           F+IRRPK+EWDPIS+NYTSGTT+RPK V+YSHRGAYLN+++TVLL+ M +  VYLW+VPM
Sbjct: 207 FEIRRPKNEWDPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTTSVYLWSVPM 266

Query: 241 FHCNGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISE- 300
           FHCNGWCF WG AAQG TNIC R V+ K IF NI  H VTH G APTVLNMI+N+P    
Sbjct: 267 FHCNGWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNL 326

Query: 301 QKPLPGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPED 360
             PLP KV VMTGG+PPP  V+ +M  +GF + H YGLTET GPA  C  KPEWD+L  +
Sbjct: 327 HTPLPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPE 386

Query: 361 QQAKLNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQE 420
           ++  L +RQGL H+ +E ++++DPVTMES  ADG T+GEVMFRGNTVMSGY KDLKAT+E
Sbjct: 387 ERYALKARQGLNHLAMEEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEE 446

Query: 421 AFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVG 480
           AF GGWFRSGDLGV+H DGYI+LKDR KD++ISGGENIS++EVE+VLYSH ++LE A+V 
Sbjct: 447 AFEGGWFRSGDLGVKHEDGYIQLKDRKKDVVISGGENISTVEVETVLYSHEAVLEAAVVA 506

Query: 481 RPDDHWGETPCAFVKLKDGC--SANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQ 540
           RPD  WGETPCAFV LK+G     + ++IIKFCR+ LPHYMAP++VVF +LPKTSTGKIQ
Sbjct: 507 RPDKLWGETPCAFVTLKEGFDNDVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQ 566

Query: 541 KFVLKEEATAMGSL 551
           K++LKE+A AMGSL
Sbjct: 567 KYILKEKAMAMGSL 572

BLAST of MC11g0055.1 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 712.2 bits (1837), Expect = 4.5e-204
Identity = 347/554 (62.64%), Postives = 427/554 (77.08%), Query Frame = 0

Query: 1   KSMEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVR 60
           KSMEG+ +CSAN V L+PITFLERS+  Y D  SLVYG VRYTW  T  RC +LASAL  
Sbjct: 27  KSMEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTT 86

Query: 61  K-GISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFV 120
             GIS GDVVA  + NIP +YELHFAVPMAG +LCTLN R+D+AMVSTLL+HSEAK+IFV
Sbjct: 87  HFGISPGDVVATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFV 146

Query: 121 DYQFLPMVKRAIKLMSKTSEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVD 180
           + Q L   + A+ L+++   K P +V++   D  S   +  D       +  LLA G  D
Sbjct: 147 EPQLLETARAALDLLAQKDIKPPTLVLLT--DSESFTSSSYD------HYNHLLANGSDD 206

Query: 181 FKIRRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPM 240
           F+IRRPK+E DPIS+NYTSGTT+RPK V+YSHRGAYLN+++TVLL+ M +  VYLW+VPM
Sbjct: 207 FEIRRPKNECDPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTRSVYLWSVPM 266

Query: 241 FHCNGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISE- 300
           FHCNGWCF WG AAQG TNIC R V+ K IF NI  H VTH G APTVLNMI+N+P    
Sbjct: 267 FHCNGWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNL 326

Query: 301 QKPLPGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPED 360
             PLP KV VMTGG+PPP  V+ +M  +GF + H YGLTET+GPAT C  KPEWD+L  +
Sbjct: 327 HTPLPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPE 386

Query: 361 QQAKLNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQE 420
           ++  L +RQGL H+ +E ++++DPV+MES  ADG T+GEVMFRGNTVMSGY KDLKAT+E
Sbjct: 387 ERYALKARQGLNHLAMEEMDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEE 446

Query: 421 AFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVG 480
           AF GGWFR+GDLGV+H DGYI+LKDR KD++ISGGEN+S++EVE+VLYSH ++LE A+V 
Sbjct: 447 AFEGGWFRTGDLGVKHEDGYIQLKDRKKDVVISGGENVSTVEVETVLYSHEAVLEAAVVA 506

Query: 481 RPDDHWGETPCAFVKLKDGC--SANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQ 540
           RPD  WGETPCAFV LK+G     + ++IIKFCR+ LPHYMAP++VVF +LPKTSTGKIQ
Sbjct: 507 RPDKLWGETPCAFVTLKEGFDNGVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQ 566

Query: 541 KFVLKEEATAMGSL 551
           K++LKE+A AMGSL
Sbjct: 567 KYILKEKAKAMGSL 572

BLAST of MC11g0055.1 vs. ExPASy Swiss-Prot
Match: Q8VZF1 (Acetate/butyrate--CoA ligase AAE7, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE7 PE=1 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 3.2e-173
Identity = 294/563 (52.22%), Postives = 396/563 (70.34%), Query Frame = 0

Query: 1   KSMEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVR 60
           + ++ + +  AN  +LTP+ FL+R+A V+  R S+++G   YTWR T  RC RLASAL  
Sbjct: 7   RDIDDLPKIPANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALAD 66

Query: 61  KGISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVD 120
           + I  G  VA +APNIPAMYE HF VPM GAVL  +N+R +A  V+ LLSHS++ +I VD
Sbjct: 67  RSIGPGSTVAIIAPNIPAMYEAHFGVPMCGAVLNCVNIRLNAPTVAFLLSHSQSSVIMVD 126

Query: 121 YQFLPMVKRAIKLMSK---TSEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGE 180
            +F  + + +++LM +   +S K PL++VI ++      +N   S    +E+E  LATG+
Sbjct: 127 QEFFTLAEDSLRLMEEKAGSSFKRPLLIVIGDHTCAPESLNRALSK-GAIEYEDFLATGD 186

Query: 181 VDFKIRRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTV 240
            ++  + P DEW  I+L YTSGTT+ PKGV+  HRGAY+  LS  L+  M    VYLWT+
Sbjct: 187 PNYPWQPPADEWQSIALGYTSGTTASPKGVVLHHRGAYIMALSNPLIWGMQDGAVYLWTL 246

Query: 241 PMFHCNGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPIS 300
           PMFHCNGWCF W +A   GT+IC R VTAKE+++ I+++ VTH   AP VLN I+NAP  
Sbjct: 247 PMFHCNGWCFPWSLAVLSGTSICLRQVTAKEVYSMIAKYKVTHFCAAPVVLNAIVNAPKE 306

Query: 301 EQ-KPLPGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLP 360
           +   PLP  V VMT GA PP  VL+ M   GF + H+YGL+ETYGP+TVC+WKPEWDSLP
Sbjct: 307 DTILPLPHTVHVMTAGAAPPPSVLFSMNQKGFRVAHTYGLSETYGPSTVCAWKPEWDSLP 366

Query: 361 EDQQAKLNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKAT 420
            + QAKLN+RQG+++ G+E +++ D  T +  PADGKT GE++FRGN VM GYLK+ +A 
Sbjct: 367 PETQAKLNARQGVRYTGMEQLDVIDTQTGKPVPADGKTAGEIVFRGNMVMKGYLKNPEAN 426

Query: 421 QEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAI 480
           +E F GGWF SGD+ V+HPD YIE+KDRSKD+IISGGENISS+EVE+V+Y HP++LE ++
Sbjct: 427 KETFAGGWFHSGDIAVKHPDNYIEIKDRSKDVIISGGENISSVEVENVVYHHPAVLEASV 486

Query: 481 VGRPDDHWGETPCAFVKLKDGCSAN-----GEEIIKFCREHLPHYMAPRSVVFRDLPKTS 540
           V RPD+ W E+PCAFV LK     +      ++I+KFCRE LP Y  P+SVVF  LPKT+
Sbjct: 487 VARPDERWQESPCAFVTLKSDYEKHDQNKLAQDIMKFCREKLPAYWVPKSVVFGPLPKTA 546

Query: 541 TGKIQKFVLKEEATAMGSLQRSK 555
           TGKIQK +L+ +A  MG + RS+
Sbjct: 547 TGKIQKHILRTKAKEMGPVPRSR 568

BLAST of MC11g0055.1 vs. NCBI nr
Match: XP_022137950.1 (probable acyl-activating enzyme 1, peroxisomal [Momordica charantia])

HSP 1 Score: 1120 bits (2898), Expect = 0.0
Identity = 553/555 (99.64%), Postives = 555/555 (100.00%), Query Frame = 0

Query: 3   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 62
           MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG
Sbjct: 1   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 60

Query: 63  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 122
           ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ
Sbjct: 61  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 120

Query: 123 FLPMVKRAIKLMSKTSEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFKI 182
           FLPMVKRAIKLMSKT+EKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFKI
Sbjct: 121 FLPMVKRAIKLMSKTTEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFKI 180

Query: 183 RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFHC 242
           RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFHC
Sbjct: 181 RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFHC 240

Query: 243 NGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKPL 302
           NGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKPL
Sbjct: 241 NGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKPL 300

Query: 303 PGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQAK 362
           PGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQAK
Sbjct: 301 PGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQAK 360

Query: 363 LNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFNG 422
           LNSRQGLQHVGLEGV+IKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFNG
Sbjct: 361 LNSRQGLQHVGLEGVDIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFNG 420

Query: 423 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPDD 482
           GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPDD
Sbjct: 421 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPDD 480

Query: 483 HWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLKE 542
           HWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLKE
Sbjct: 481 HWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLKE 540

Query: 543 EATAMGSLQRSKHSS 557
           EATAMGSLQRSKHSS
Sbjct: 541 EATAMGSLQRSKHSS 555

BLAST of MC11g0055.1 vs. NCBI nr
Match: XP_022132725.1 (probable acyl-activating enzyme 1, peroxisomal [Momordica charantia])

HSP 1 Score: 975 bits (2520), Expect = 0.0
Identity = 474/550 (86.18%), Postives = 511/550 (92.91%), Query Frame = 0

Query: 3   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 62
           M+G +RCSAN V LTPITFLERSAAVYGDR SLVYGR+RYTW+DTLQRCTRLASALVR G
Sbjct: 1   MDGADRCSANHVPLTPITFLERSAAVYGDRISLVYGRLRYTWKDTLQRCTRLASALVRLG 60

Query: 63  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 122
           I+ GDVVAALAPNIPAMYELHFAVPMAGAVLCTLN+RHDAAMVSTLL+HSEAKII VDYQ
Sbjct: 61  IAPGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNIRHDAAMVSTLLTHSEAKIIIVDYQ 120

Query: 123 FLPMVKRAIKLMSKTSEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFKI 182
           FL +VK AI++MS+  EKLPLVV+IQEYDQPSS  +   SA EDLEFE LLATGE+DF+I
Sbjct: 121 FLHIVKGAIEVMSQRKEKLPLVVIIQEYDQPSSHKDRAGSASEDLEFEKLLATGELDFEI 180

Query: 183 RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFHC 242
           RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLN+LSTVLLN M S P+YLWTVPMFHC
Sbjct: 181 RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNSLSTVLLNGMCSNPIYLWTVPMFHC 240

Query: 243 NGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKPL 302
           NGWCF+W VAAQGGTN+CQR VTAKEIF NISQH VTHM GAPTVLNMIINAPISEQKPL
Sbjct: 241 NGWCFTWAVAAQGGTNVCQRNVTAKEIFDNISQHKVTHMAGAPTVLNMIINAPISEQKPL 300

Query: 303 PGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQAK 362
           P KVTVMTGGAPPPSHVLYKMRALGF ++HSYGLTETYGPATVC WKPEWDSLP+D+QAK
Sbjct: 301 PEKVTVMTGGAPPPSHVLYKMRALGFFVVHSYGLTETYGPATVCVWKPEWDSLPQDKQAK 360

Query: 363 LNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFNG 422
           LNSRQGLQHVG+E V+IKDPVTMES PADGKTMGEVMFRGNTVM+GYLKDLKAT+EAFNG
Sbjct: 361 LNSRQGLQHVGMEEVDIKDPVTMESVPADGKTMGEVMFRGNTVMNGYLKDLKATREAFNG 420

Query: 423 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPDD 482
           GWFRSGDLGVRH DGYIELKDRSKDIIISGGENISSIEVESVL+SHP++LE A+VGRPDD
Sbjct: 421 GWFRSGDLGVRHLDGYIELKDRSKDIIISGGENISSIEVESVLFSHPAVLEAAVVGRPDD 480

Query: 483 HWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLKE 542
           HWGETPCAFVKLKDGCSA  EEII+FCREHLPHYM+PR VVF+DLPKTSTGK QKF+LK+
Sbjct: 481 HWGETPCAFVKLKDGCSATEEEIIEFCREHLPHYMSPRCVVFKDLPKTSTGKTQKFILKQ 540

Query: 543 EATAMGSLQR 552
           EA AMGSL +
Sbjct: 541 EAKAMGSLSK 550

BLAST of MC11g0055.1 vs. NCBI nr
Match: KAG7024112.1 (putative acyl-activating enzyme 1, peroxisomal [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 954 bits (2465), Expect = 0.0
Identity = 468/550 (85.09%), Postives = 506/550 (92.00%), Query Frame = 0

Query: 3   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 62
           M+GM+RCSAN + LTPITFLERSAAVYGDR SLVYGRV+YTWR+TL RCTRLASALVR G
Sbjct: 1   MDGMDRCSANYLPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 63  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 122
           I+RGDVVAALAPNIPAMYELHFAVPMAGAVLC+LN+RHDAAMVSTLLSHSEAKII VDYQ
Sbjct: 61  IARGDVVAALAPNIPAMYELHFAVPMAGAVLCSLNVRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 123 FLPMVKRAIKLMSKTSEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFKI 182
           F  +V  AIK MS+  EK+PLVV+I EYDQP S I+   SA   LEFESLL +G++DFKI
Sbjct: 121 FEHIVTGAIKAMSERREKIPLVVIINEYDQPPSHIDREGSASGYLEFESLLESGKLDFKI 180

Query: 183 RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFHC 242
           RRPKDE DPI+LNYTSGTTSRPKGVIYSHRGAYLN LS VLLNDM SLPVYLW VPMFHC
Sbjct: 181 RRPKDELDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWVVPMFHC 240

Query: 243 NGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKPL 302
           NGW  +WGVAAQ GTNICQR  +AKEIF NIS H VTHMGGAPTVLNMIINAP SEQKPL
Sbjct: 241 NGWSLTWGVAAQSGTNICQRNASAKEIFDNISLHKVTHMGGAPTVLNMIINAPTSEQKPL 300

Query: 303 PGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQAK 362
           PGKVT+MTGGAPPPSHVL+KMRA+GFLI+H+YGLTETYGPATVCSWKPEWDSLP+D+QAK
Sbjct: 301 PGKVTMMTGGAPPPSHVLFKMRAMGFLIVHAYGLTETYGPATVCSWKPEWDSLPQDKQAK 360

Query: 363 LNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFNG 422
           L+SRQGLQHVGLE V+IKDPVTMESAPADGKT+GEVM RGNTVMSGYLKDLKAT+EAFNG
Sbjct: 361 LSSRQGLQHVGLEEVDIKDPVTMESAPADGKTIGEVMVRGNTVMSGYLKDLKATREAFNG 420

Query: 423 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPDD 482
           GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVL+SHPS+LE A+VGRPDD
Sbjct: 421 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFSHPSVLEAAVVGRPDD 480

Query: 483 HWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLKE 542
           HWGETPCAFVKLKDGCSA+ E+IIKFCREHLPHYMAPRSVVF+DLPKTSTGK QKF+LKE
Sbjct: 481 HWGETPCAFVKLKDGCSASEEDIIKFCREHLPHYMAPRSVVFKDLPKTSTGKTQKFILKE 540

Query: 543 EATAMGSLQR 552
           EA AMGSL +
Sbjct: 541 EAKAMGSLPK 550

BLAST of MC11g0055.1 vs. NCBI nr
Match: XP_022937090.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 954 bits (2465), Expect = 0.0
Identity = 468/550 (85.09%), Postives = 506/550 (92.00%), Query Frame = 0

Query: 3   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 62
           M+GM+RCSAN + LTPITFLERSAAVYGDR SLVYGRV+YTWR+TL RCTRLASALVR G
Sbjct: 1   MDGMDRCSANYLPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 63  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 122
           I+RGDVVAALAPNIPAMYELHFAVPMAGAVLC+LN+RHDAAMVSTLLSHSEAKII VDYQ
Sbjct: 61  IARGDVVAALAPNIPAMYELHFAVPMAGAVLCSLNVRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 123 FLPMVKRAIKLMSKTSEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFKI 182
           F  +V  AIK MS+  EK+PLVV+I EYDQP S I+   SA   LEFESLL +G++DFKI
Sbjct: 121 FEHIVMGAIKAMSERREKIPLVVIINEYDQPPSHIDREGSASGYLEFESLLESGKLDFKI 180

Query: 183 RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFHC 242
           RRPKDE DPI+LNYTSGTTSRPKGVIYSHRGAYLN LS VLLNDM SLPVYLW VPMFHC
Sbjct: 181 RRPKDELDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWVVPMFHC 240

Query: 243 NGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKPL 302
           NGW  +WGVAAQ GTNICQR  +AKEIF NIS H VTHMGGAPTVLNMIINAP SEQKPL
Sbjct: 241 NGWSLTWGVAAQSGTNICQRNASAKEIFDNISLHKVTHMGGAPTVLNMIINAPTSEQKPL 300

Query: 303 PGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQAK 362
           PGKVT+MTGGAPPPSHVL+KMRA+GFLI+H+YGLTETYGPATVCSWKPEWDSLP+D+QAK
Sbjct: 301 PGKVTMMTGGAPPPSHVLFKMRAMGFLIVHAYGLTETYGPATVCSWKPEWDSLPQDKQAK 360

Query: 363 LNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFNG 422
           L+SRQGLQHVGLE V+IKDPVTMESAPADGKT+GEVM RGNTVMSGYLKDLKAT+EAFNG
Sbjct: 361 LSSRQGLQHVGLEEVDIKDPVTMESAPADGKTIGEVMVRGNTVMSGYLKDLKATREAFNG 420

Query: 423 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPDD 482
           GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVL+SHPS+LE A+VGRPDD
Sbjct: 421 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFSHPSVLEAAVVGRPDD 480

Query: 483 HWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLKE 542
           HWGETPCAFVKLKDGCSA+ E+IIKFCREHLPHYMAPRSVVF+DLPKTSTGK QKF+LKE
Sbjct: 481 HWGETPCAFVKLKDGCSASEEDIIKFCREHLPHYMAPRSVVFKDLPKTSTGKTQKFILKE 540

Query: 543 EATAMGSLQR 552
           EA AMGSL +
Sbjct: 541 EAKAMGSLPK 550

BLAST of MC11g0055.1 vs. NCBI nr
Match: KAG6591226.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 952 bits (2460), Expect = 0.0
Identity = 467/550 (84.91%), Postives = 505/550 (91.82%), Query Frame = 0

Query: 3   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 62
           M+GM+RCSAN + LTPITFLERSAAVYGDR SLVYGRV+YTWR+TL RCTRLASALVR G
Sbjct: 1   MDGMDRCSANYLPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 63  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 122
           I+RGDVVAALAPNIPAMYELHFAVPMAGAVLC+LN+RHDAAMVSTLLSHSEAKII VDYQ
Sbjct: 61  IARGDVVAALAPNIPAMYELHFAVPMAGAVLCSLNVRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 123 FLPMVKRAIKLMSKTSEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFKI 182
           F  +V  AIK MS+  EK+PLVV+I EYDQP S I+   SA   LEFE LL +G++DFKI
Sbjct: 121 FEHIVTGAIKAMSERREKIPLVVIINEYDQPPSHIDREGSASGYLEFERLLESGKLDFKI 180

Query: 183 RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFHC 242
           RRPKDE DPI+LNYTSGTTSRPKGVIYSHRGAYLN LS VLLNDM SLPVYLW VPMFHC
Sbjct: 181 RRPKDELDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWVVPMFHC 240

Query: 243 NGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKPL 302
           NGW  +WGVAAQ GTNICQR  +AKEIF NIS H VTHMGGAPTVLNMIINAP SEQKPL
Sbjct: 241 NGWSLTWGVAAQSGTNICQRNASAKEIFDNISLHKVTHMGGAPTVLNMIINAPTSEQKPL 300

Query: 303 PGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQAK 362
           PGKVT+MTGGAPPPSHVL+KMRA+GFLI+H+YGLTETYGPATVCSWKPEWDSLP+D+QAK
Sbjct: 301 PGKVTMMTGGAPPPSHVLFKMRAMGFLIVHAYGLTETYGPATVCSWKPEWDSLPQDKQAK 360

Query: 363 LNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFNG 422
           L+SRQGLQHVGLE V+IKDPVTMESAPADGKT+GEVM RGNTVMSGYLKDLKAT+EAFNG
Sbjct: 361 LSSRQGLQHVGLEEVDIKDPVTMESAPADGKTIGEVMVRGNTVMSGYLKDLKATREAFNG 420

Query: 423 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPDD 482
           GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVL+SHPS+LE A+VGRPDD
Sbjct: 421 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFSHPSVLEAAVVGRPDD 480

Query: 483 HWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLKE 542
           HWGETPCAFVKLKDGCSA+ E+IIKFCREHLPHYMAPRSVVF+DLPKTSTGK QKF+LKE
Sbjct: 481 HWGETPCAFVKLKDGCSASEEDIIKFCREHLPHYMAPRSVVFKDLPKTSTGKTQKFILKE 540

Query: 543 EATAMGSLQR 552
           EA AMGSL +
Sbjct: 541 EAKAMGSLPK 550

BLAST of MC11g0055.1 vs. ExPASy TrEMBL
Match: A0A6J1C834 (probable acyl-activating enzyme 1, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111009236 PE=4 SV=1)

HSP 1 Score: 1120 bits (2898), Expect = 0.0
Identity = 553/555 (99.64%), Postives = 555/555 (100.00%), Query Frame = 0

Query: 3   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 62
           MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG
Sbjct: 1   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 60

Query: 63  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 122
           ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ
Sbjct: 61  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 120

Query: 123 FLPMVKRAIKLMSKTSEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFKI 182
           FLPMVKRAIKLMSKT+EKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFKI
Sbjct: 121 FLPMVKRAIKLMSKTTEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFKI 180

Query: 183 RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFHC 242
           RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFHC
Sbjct: 181 RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFHC 240

Query: 243 NGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKPL 302
           NGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKPL
Sbjct: 241 NGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKPL 300

Query: 303 PGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQAK 362
           PGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQAK
Sbjct: 301 PGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQAK 360

Query: 363 LNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFNG 422
           LNSRQGLQHVGLEGV+IKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFNG
Sbjct: 361 LNSRQGLQHVGLEGVDIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFNG 420

Query: 423 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPDD 482
           GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPDD
Sbjct: 421 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPDD 480

Query: 483 HWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLKE 542
           HWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLKE
Sbjct: 481 HWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLKE 540

Query: 543 EATAMGSLQRSKHSS 557
           EATAMGSLQRSKHSS
Sbjct: 541 EATAMGSLQRSKHSS 555

BLAST of MC11g0055.1 vs. ExPASy TrEMBL
Match: A0A6J1BTA0 (probable acyl-activating enzyme 1, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111005519 PE=4 SV=1)

HSP 1 Score: 975 bits (2520), Expect = 0.0
Identity = 474/550 (86.18%), Postives = 511/550 (92.91%), Query Frame = 0

Query: 3   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 62
           M+G +RCSAN V LTPITFLERSAAVYGDR SLVYGR+RYTW+DTLQRCTRLASALVR G
Sbjct: 1   MDGADRCSANHVPLTPITFLERSAAVYGDRISLVYGRLRYTWKDTLQRCTRLASALVRLG 60

Query: 63  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 122
           I+ GDVVAALAPNIPAMYELHFAVPMAGAVLCTLN+RHDAAMVSTLL+HSEAKII VDYQ
Sbjct: 61  IAPGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNIRHDAAMVSTLLTHSEAKIIIVDYQ 120

Query: 123 FLPMVKRAIKLMSKTSEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFKI 182
           FL +VK AI++MS+  EKLPLVV+IQEYDQPSS  +   SA EDLEFE LLATGE+DF+I
Sbjct: 121 FLHIVKGAIEVMSQRKEKLPLVVIIQEYDQPSSHKDRAGSASEDLEFEKLLATGELDFEI 180

Query: 183 RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFHC 242
           RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLN+LSTVLLN M S P+YLWTVPMFHC
Sbjct: 181 RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNSLSTVLLNGMCSNPIYLWTVPMFHC 240

Query: 243 NGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKPL 302
           NGWCF+W VAAQGGTN+CQR VTAKEIF NISQH VTHM GAPTVLNMIINAPISEQKPL
Sbjct: 241 NGWCFTWAVAAQGGTNVCQRNVTAKEIFDNISQHKVTHMAGAPTVLNMIINAPISEQKPL 300

Query: 303 PGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQAK 362
           P KVTVMTGGAPPPSHVLYKMRALGF ++HSYGLTETYGPATVC WKPEWDSLP+D+QAK
Sbjct: 301 PEKVTVMTGGAPPPSHVLYKMRALGFFVVHSYGLTETYGPATVCVWKPEWDSLPQDKQAK 360

Query: 363 LNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFNG 422
           LNSRQGLQHVG+E V+IKDPVTMES PADGKTMGEVMFRGNTVM+GYLKDLKAT+EAFNG
Sbjct: 361 LNSRQGLQHVGMEEVDIKDPVTMESVPADGKTMGEVMFRGNTVMNGYLKDLKATREAFNG 420

Query: 423 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPDD 482
           GWFRSGDLGVRH DGYIELKDRSKDIIISGGENISSIEVESVL+SHP++LE A+VGRPDD
Sbjct: 421 GWFRSGDLGVRHLDGYIELKDRSKDIIISGGENISSIEVESVLFSHPAVLEAAVVGRPDD 480

Query: 483 HWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLKE 542
           HWGETPCAFVKLKDGCSA  EEII+FCREHLPHYM+PR VVF+DLPKTSTGK QKF+LK+
Sbjct: 481 HWGETPCAFVKLKDGCSATEEEIIEFCREHLPHYMSPRCVVFKDLPKTSTGKTQKFILKQ 540

Query: 543 EATAMGSLQR 552
           EA AMGSL +
Sbjct: 541 EAKAMGSLSK 550

BLAST of MC11g0055.1 vs. ExPASy TrEMBL
Match: A0A6J1FFJ8 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111443496 PE=4 SV=1)

HSP 1 Score: 954 bits (2465), Expect = 0.0
Identity = 468/550 (85.09%), Postives = 506/550 (92.00%), Query Frame = 0

Query: 3   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 62
           M+GM+RCSAN + LTPITFLERSAAVYGDR SLVYGRV+YTWR+TL RCTRLASALVR G
Sbjct: 1   MDGMDRCSANYLPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 63  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 122
           I+RGDVVAALAPNIPAMYELHFAVPMAGAVLC+LN+RHDAAMVSTLLSHSEAKII VDYQ
Sbjct: 61  IARGDVVAALAPNIPAMYELHFAVPMAGAVLCSLNVRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 123 FLPMVKRAIKLMSKTSEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFKI 182
           F  +V  AIK MS+  EK+PLVV+I EYDQP S I+   SA   LEFESLL +G++DFKI
Sbjct: 121 FEHIVMGAIKAMSERREKIPLVVIINEYDQPPSHIDREGSASGYLEFESLLESGKLDFKI 180

Query: 183 RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFHC 242
           RRPKDE DPI+LNYTSGTTSRPKGVIYSHRGAYLN LS VLLNDM SLPVYLW VPMFHC
Sbjct: 181 RRPKDELDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWVVPMFHC 240

Query: 243 NGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKPL 302
           NGW  +WGVAAQ GTNICQR  +AKEIF NIS H VTHMGGAPTVLNMIINAP SEQKPL
Sbjct: 241 NGWSLTWGVAAQSGTNICQRNASAKEIFDNISLHKVTHMGGAPTVLNMIINAPTSEQKPL 300

Query: 303 PGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQAK 362
           PGKVT+MTGGAPPPSHVL+KMRA+GFLI+H+YGLTETYGPATVCSWKPEWDSLP+D+QAK
Sbjct: 301 PGKVTMMTGGAPPPSHVLFKMRAMGFLIVHAYGLTETYGPATVCSWKPEWDSLPQDKQAK 360

Query: 363 LNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFNG 422
           L+SRQGLQHVGLE V+IKDPVTMESAPADGKT+GEVM RGNTVMSGYLKDLKAT+EAFNG
Sbjct: 361 LSSRQGLQHVGLEEVDIKDPVTMESAPADGKTIGEVMVRGNTVMSGYLKDLKATREAFNG 420

Query: 423 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPDD 482
           GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVL+SHPS+LE A+VGRPDD
Sbjct: 421 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFSHPSVLEAAVVGRPDD 480

Query: 483 HWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLKE 542
           HWGETPCAFVKLKDGCSA+ E+IIKFCREHLPHYMAPRSVVF+DLPKTSTGK QKF+LKE
Sbjct: 481 HWGETPCAFVKLKDGCSASEEDIIKFCREHLPHYMAPRSVVFKDLPKTSTGKTQKFILKE 540

Query: 543 EATAMGSLQR 552
           EA AMGSL +
Sbjct: 541 EAKAMGSLPK 550

BLAST of MC11g0055.1 vs. ExPASy TrEMBL
Match: A0A6J1BUN5 (probable acyl-activating enzyme 1, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111005527 PE=4 SV=1)

HSP 1 Score: 949 bits (2452), Expect = 0.0
Identity = 469/553 (84.81%), Postives = 508/553 (91.86%), Query Frame = 0

Query: 3   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 62
           M+GMERCSAN   LTPITFLERSAAVY DR SLVYG +RYTWRDTLQRCTRLAS LVR G
Sbjct: 1   MDGMERCSANHAPLTPITFLERSAAVYADRISLVYGHLRYTWRDTLQRCTRLASVLVRLG 60

Query: 63  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 122
           I+RGDVVAALAPN+PAMYELHFAVPMAGAVLCTLN+RH+AAMVSTLL+HSEAKII VDYQ
Sbjct: 61  IARGDVVAALAPNVPAMYELHFAVPMAGAVLCTLNIRHNAAMVSTLLTHSEAKIIIVDYQ 120

Query: 123 FLPMVKRAIKLMSKTSEK-LPLVVVIQEYDQ--PSSCINGVDSALEDLEFESLLATGEVD 182
           FL +VK AIK+M +  EK  PLVV+IQE+ Q  PS  I+      + LEFESLLATGEVD
Sbjct: 121 FLHVVKGAIKVMFERREKKAPLVVIIQEFGQGRPSDSIDP-----DHLEFESLLATGEVD 180

Query: 183 FKIRRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPM 242
           FKIRRP+DEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDM  LPVYLWTVPM
Sbjct: 181 FKIRRPRDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMSPLPVYLWTVPM 240

Query: 243 FHCNGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQ 302
           FHCNGWCF+WGVAAQGGTNICQRTVTAKEIFANIS+HNVTHMGGAPTVLNMIINAPI EQ
Sbjct: 241 FHCNGWCFTWGVAAQGGTNICQRTVTAKEIFANISRHNVTHMGGAPTVLNMIINAPIIEQ 300

Query: 303 KPLPGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQ 362
           KPLPGKVTVMTGGAPPPSHVL KMRA+GF ++H+YGLTETYG ATVCSW+PEWDSL ED+
Sbjct: 301 KPLPGKVTVMTGGAPPPSHVLCKMRAMGFFVVHTYGLTETYGAATVCSWRPEWDSLHEDK 360

Query: 363 QAKLNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEA 422
           QAKLNSRQGLQHVG+E ++IK+P+TM+S PADGKTMGEVMFRGNTVM+GYLKDLKAT+EA
Sbjct: 361 QAKLNSRQGLQHVGMEEMDIKNPITMKSVPADGKTMGEVMFRGNTVMNGYLKDLKATREA 420

Query: 423 FNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGR 482
           F+GGWFRSGDLGVRHP+GYIELKDRSKDIIISGGENISSIEVESVL+SHPSILE A+VGR
Sbjct: 421 FSGGWFRSGDLGVRHPNGYIELKDRSKDIIISGGENISSIEVESVLFSHPSILEAAVVGR 480

Query: 483 PDDHWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFV 542
           PDDHWGETPCAFVKLKDG SA+ EEIIKFCREHL HYMAPR VVF+DLPKTSTGK QKF+
Sbjct: 481 PDDHWGETPCAFVKLKDGRSASEEEIIKFCREHLSHYMAPRCVVFKDLPKTSTGKTQKFI 540

Query: 543 LKEEATAMGSLQR 552
           LKEEA AMGSL +
Sbjct: 541 LKEEARAMGSLPK 548

BLAST of MC11g0055.1 vs. ExPASy TrEMBL
Match: A0A6J1IF13 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111473618 PE=4 SV=1)

HSP 1 Score: 948 bits (2451), Expect = 0.0
Identity = 467/550 (84.91%), Postives = 503/550 (91.45%), Query Frame = 0

Query: 3   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 62
           M+GM+RCSAN V LTPITFLERSAAVYGDR SL YGRV+YTWR+TL RCTRLASALVR G
Sbjct: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLAYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 63  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 122
           I+RGDVVAALAPNIPAMYELHFAVPMAGAVLC+LN+RHDAAMVSTLLSHSEAKII VDYQ
Sbjct: 61  IARGDVVAALAPNIPAMYELHFAVPMAGAVLCSLNVRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 123 FLPMVKRAIKLMSKTSEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFKI 182
           F  +V  AIK MS+  EK+PLVV+I EYDQP S I+   SA E LEFESLL +G++DFKI
Sbjct: 121 FEHIVMGAIKAMSERREKIPLVVIINEYDQPPSYIDREGSASEYLEFESLLESGKLDFKI 180

Query: 183 RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFHC 242
           RRPK E DPI+LNYTSGTTSRPKGVIYSHRGAYLN LS VLLNDM SLPVYLW VPMFHC
Sbjct: 181 RRPKYELDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWVVPMFHC 240

Query: 243 NGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKPL 302
           NGW  +WGVAAQ GTNICQR V+AKEIF NIS H VTHMGGAPTVLNMIINAP SEQKPL
Sbjct: 241 NGWSLTWGVAAQSGTNICQRNVSAKEIFENISLHKVTHMGGAPTVLNMIINAPTSEQKPL 300

Query: 303 PGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQAK 362
           P KVT+MTGGAPPPSHVL+KMRA+GFLI+H+YGLTETYGPATVCSWKPEWDSLP+D+QAK
Sbjct: 301 PRKVTMMTGGAPPPSHVLFKMRAMGFLIVHAYGLTETYGPATVCSWKPEWDSLPQDKQAK 360

Query: 363 LNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFNG 422
           L SRQGLQHVGLE V+IKDPVTMESAPADGKT+GEVM RGNTVMSGYLKDLKAT+EAFNG
Sbjct: 361 LTSRQGLQHVGLEEVDIKDPVTMESAPADGKTIGEVMVRGNTVMSGYLKDLKATREAFNG 420

Query: 423 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPDD 482
           GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVL+SHPS+LE A+VGRPDD
Sbjct: 421 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFSHPSVLEAAVVGRPDD 480

Query: 483 HWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLKE 542
            WGETPCAFVKLKDGCSA+ E+IIKFCREHLPHYMAPRSVVF+DLPKTSTGK QKF+LKE
Sbjct: 481 RWGETPCAFVKLKDGCSASEEDIIKFCREHLPHYMAPRSVVFKDLPKTSTGKTQKFILKE 540

Query: 543 EATAMGSLQR 552
           EA AMGSL +
Sbjct: 541 EAKAMGSLPK 550

BLAST of MC11g0055.1 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 786.2 bits (2029), Expect = 1.8e-227
Identity = 367/554 (66.25%), Postives = 451/554 (81.41%), Query Frame = 0

Query: 3   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 62
           MEG  +  AN V LTPI+FL+RSA VY DR S+VYG V+YTWR T  RC R+ASAL + G
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 63  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 122
           IS GDVV+ LAPN+PAM ELHF VPMAGA+LCTLN+RHD+++V+ LL HS  K+IF D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 123 FLPMVKRAIKLMSKTSEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFKI 182
           FL + + A +++S   +K+P++V+I E    +  ++    + E +E+E ++A G+ DF++
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPE--PLTQSVSRKKRSEEMMEYEDVVAMGKSDFEV 182

Query: 183 RRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFHC 242
            RP DE D IS+NYTSGTTS PKGV+YSHRGAYLN+L+ VLLN+M S P YLWT PMFHC
Sbjct: 183 IRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHC 242

Query: 243 NGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKPL 302
           NGWC  WGV A GGTNIC R VTAK IF NISQH VTHMGGAPT+LNMIINAP SEQKPL
Sbjct: 243 NGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPL 302

Query: 303 PGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQAK 362
           PGKV+ +TG APPP+HV++KM  LGF + HSYGLTETYGP T+C+WKPEWDSLP ++QAK
Sbjct: 303 PGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAK 362

Query: 363 LNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFNG 422
           + +RQG+ H+GLE +++KDPVTM + PADG TMGEV+FRGNTVM+GYLK+ +AT+EAF G
Sbjct: 363 MKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKG 422

Query: 423 GWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPDD 482
           GWF SGDLGV+HPDGYIELKDRSKDIIISGGENISSIEVES L++HP +LE A+V RPD+
Sbjct: 423 GWFWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDE 482

Query: 483 HWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLKE 542
           +WGET CAFVKLKDG  A+ EE+I +CR+ LPHYMAPRS+VF DLPKTSTGK+QKFVL+ 
Sbjct: 483 YWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRT 542

Query: 543 EATAMGSLQRSKHS 557
           +A A+ SL +   S
Sbjct: 543 KAKALVSLSKKGRS 554

BLAST of MC11g0055.1 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 743.0 bits (1917), Expect = 1.7e-214
Identity = 354/558 (63.44%), Postives = 439/558 (78.67%), Query Frame = 0

Query: 1   KSMEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVR 60
           +++EG+ R  AN   L+PITFLERSA VY DR SLV+G V++TW  T QRC RLASAL  
Sbjct: 46  RTIEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTN 105

Query: 61  KGISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVD 120
            GISRGDVVAALAPN+PAM+ELHFAVPMAG +LC LN R D + +S LL+HSEAKI+FVD
Sbjct: 106 LGISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVD 165

Query: 121 YQFLPMVKRAIKLMS---KTSEKLPLVVVIQEYDQPSSCINGVDSALE----DLEFESLL 180
           +Q L +   A+ L++   KT + L LV++ Q  D   S  +   +       D E+E+LL
Sbjct: 166 HQLLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLL 225

Query: 181 ATGEVDFKIRRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVY 240
            +G+ +F+I +P+ EWDPIS+NYTSGTTSRPKGV+YSHRGAYLN+L+TV L+ M   PVY
Sbjct: 226 KSGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVY 285

Query: 241 LWTVPMFHCNGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIIN 300
           LWTVPMFHCNGWC  WGVAAQGGTNIC R V+ K IF NI+ H VTHMGGAPTVLNMI+N
Sbjct: 286 LWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVN 345

Query: 301 APISEQKPLPGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWD 360
             ++E KPLP +V +MTGG+PP   +L KM  LGF + H YGLTETYGP T C WKPEWD
Sbjct: 346 YTVTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWD 405

Query: 361 SLPEDQQAKLNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDL 420
           SL  +++ KL +RQG+QH+GLEG+++KDP+TME+ P DG TMGEVMFRGNTVMSGY KD+
Sbjct: 406 SLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDI 465

Query: 421 KATQEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILE 480
           +AT++AF G WF SGDL V++PDGYIE+KDR KD+IISGGENISS+EVE VL SH ++LE
Sbjct: 466 EATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLE 525

Query: 481 VAIVGRPDDHWGETPCAFVKLKDGC-SANGEEIIKFCREHLPHYMAPRSVVFRDLPKTST 540
            A+V RPD HWG+TPC FVKLK+G  +   EEII FCR+HLPHYMAP+++VF D+PKTST
Sbjct: 526 AAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTST 585

Query: 541 GKIQKFVLKEEATAMGSL 551
           GK+QK++L+++A  MGSL
Sbjct: 586 GKVQKYLLRKKADEMGSL 603

BLAST of MC11g0055.1 vs. TAIR 10
Match: AT1G20560.2 (acyl activating enzyme 1 )

HSP 1 Score: 688.7 bits (1776), Expect = 3.8e-198
Identity = 318/478 (66.53%), Postives = 393/478 (82.22%), Query Frame = 0

Query: 79  MYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQFLPMVKRAIKLMSKTS 138
           M ELHF VPMAGA+LCTLN+RHD+++V+ LL HS  K+IF D+QFL + + A +++S   
Sbjct: 1   MVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKG 60

Query: 139 EKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFKIRRPKDEWDPISLNYTS 198
           +K+P++V+I E    +  ++    + E +E+E ++A G+ DF++ RP DE D IS+NYTS
Sbjct: 61  DKVPILVLIPE--PLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAISVNYTS 120

Query: 199 GTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFHCNGWCFSWGVAAQGGTN 258
           GTTS PKGV+YSHRGAYLN+L+ VLLN+M S P YLWT PMFHCNGWC  WGV A GGTN
Sbjct: 121 GTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNGWCLLWGVTAIGGTN 180

Query: 259 ICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKPLPGKVTVMTGGAPPPSH 318
           IC R VTAK IF NISQH VTHMGGAPT+LNMIINAP SEQKPLPGKV+ +TG APPP+H
Sbjct: 181 ICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPGKVSFITGAAPPPAH 240

Query: 319 VLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQAKLNSRQGLQHVGLEGVE 378
           V++KM  LGF + HSYGLTETYGP T+C+WKPEWDSLP ++QAK+ +RQG+ H+GLE ++
Sbjct: 241 VIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHLGLEEIQ 300

Query: 379 IKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFNGGWFRSGDLGVRHPDGY 438
           +KDPVTM + PADG TMGEV+FRGNTVM+GYLK+ +AT+EAF GGWF SGDLGV+HPDGY
Sbjct: 301 VKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGWFWSGDLGVKHPDGY 360

Query: 439 IELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPDDHWGETPCAFVKLKDGC 498
           IELKDRSKDIIISGGENISSIEVES L++HP +LE A+V RPD++WGET CAFVKLKDG 
Sbjct: 361 IELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFVKLKDGS 420

Query: 499 SANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLKEEATAMGSLQRSKHS 557
            A+ EE+I +CR+ LPHYMAPRS+VF DLPKTSTGK+QKFVL+ +A A+ SL +   S
Sbjct: 421 KASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKALVSLSKKGRS 476

BLAST of MC11g0055.1 vs. TAIR 10
Match: AT3G16910.1 (acyl-activating enzyme 7 )

HSP 1 Score: 609.8 bits (1571), Expect = 2.2e-174
Identity = 294/563 (52.22%), Postives = 396/563 (70.34%), Query Frame = 0

Query: 1   KSMEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVR 60
           + ++ + +  AN  +LTP+ FL+R+A V+  R S+++G   YTWR T  RC RLASAL  
Sbjct: 7   RDIDDLPKIPANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALAD 66

Query: 61  KGISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVD 120
           + I  G  VA +APNIPAMYE HF VPM GAVL  +N+R +A  V+ LLSHS++ +I VD
Sbjct: 67  RSIGPGSTVAIIAPNIPAMYEAHFGVPMCGAVLNCVNIRLNAPTVAFLLSHSQSSVIMVD 126

Query: 121 YQFLPMVKRAIKLMSK---TSEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGE 180
            +F  + + +++LM +   +S K PL++VI ++      +N   S    +E+E  LATG+
Sbjct: 127 QEFFTLAEDSLRLMEEKAGSSFKRPLLIVIGDHTCAPESLNRALSK-GAIEYEDFLATGD 186

Query: 181 VDFKIRRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTV 240
            ++  + P DEW  I+L YTSGTT+ PKGV+  HRGAY+  LS  L+  M    VYLWT+
Sbjct: 187 PNYPWQPPADEWQSIALGYTSGTTASPKGVVLHHRGAYIMALSNPLIWGMQDGAVYLWTL 246

Query: 241 PMFHCNGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPIS 300
           PMFHCNGWCF W +A   GT+IC R VTAKE+++ I+++ VTH   AP VLN I+NAP  
Sbjct: 247 PMFHCNGWCFPWSLAVLSGTSICLRQVTAKEVYSMIAKYKVTHFCAAPVVLNAIVNAPKE 306

Query: 301 EQ-KPLPGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLP 360
           +   PLP  V VMT GA PP  VL+ M   GF + H+YGL+ETYGP+TVC+WKPEWDSLP
Sbjct: 307 DTILPLPHTVHVMTAGAAPPPSVLFSMNQKGFRVAHTYGLSETYGPSTVCAWKPEWDSLP 366

Query: 361 EDQQAKLNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKAT 420
            + QAKLN+RQG+++ G+E +++ D  T +  PADGKT GE++FRGN VM GYLK+ +A 
Sbjct: 367 PETQAKLNARQGVRYTGMEQLDVIDTQTGKPVPADGKTAGEIVFRGNMVMKGYLKNPEAN 426

Query: 421 QEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAI 480
           +E F GGWF SGD+ V+HPD YIE+KDRSKD+IISGGENISS+EVE+V+Y HP++LE ++
Sbjct: 427 KETFAGGWFHSGDIAVKHPDNYIEIKDRSKDVIISGGENISSVEVENVVYHHPAVLEASV 486

Query: 481 VGRPDDHWGETPCAFVKLKDGCSAN-----GEEIIKFCREHLPHYMAPRSVVFRDLPKTS 540
           V RPD+ W E+PCAFV LK     +      ++I+KFCRE LP Y  P+SVVF  LPKT+
Sbjct: 487 VARPDERWQESPCAFVTLKSDYEKHDQNKLAQDIMKFCREKLPAYWVPKSVVFGPLPKTA 546

Query: 541 TGKIQKFVLKEEATAMGSLQRSK 555
           TGKIQK +L+ +A  MG + RS+
Sbjct: 547 TGKIQKHILRTKAKEMGPVPRSR 568

BLAST of MC11g0055.1 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 582.4 bits (1500), Expect = 3.8e-166
Identity = 279/554 (50.36%), Postives = 388/554 (70.04%), Query Frame = 0

Query: 3   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 62
           M+ +  C AN V LTPITFL+R++  Y +R S++YG+ R+TW  T  RC RLA++L+   
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 63  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 122
           I+R DVV+ LAPN+PAMYE+HF+VPM GAVL  +N R DA  ++ +L H+E KI+FVDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 123 FLPMVKRAIKLM-SKTSEKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGE---- 182
           F P+++  ++L+ +  S+  P +++I E D  +   +      ++L++E L+  GE    
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFS------KELDYEGLIRKGEPTPS 180

Query: 183 VDFKIRRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTV 242
               + R  +E DPISLNYTSGTT+ PKGV+ SH+GAYL+ LS+++  +M   PVYLWT+
Sbjct: 181 SSASMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTL 240

Query: 243 PMFHCNGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPIS 302
           PMFHCNGW  +W VAA+GGTN+C R VTA EI+ NI  H VTHM   PTV   ++    +
Sbjct: 241 PMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRT 300

Query: 303 EQKPLPGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPE 362
           +Q P    V V+TGG+ PP+ ++ K+  LGF ++H YGLTE  GP   C W+ EW+ LPE
Sbjct: 301 DQSPKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPE 360

Query: 363 DQQAKLNSRQGLQHVGLEGVEIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQ 422
            QQ +L  RQG++++ L  V++K+  T+ES P DGKTMGE++ +G+++M GYLK+ KAT 
Sbjct: 361 HQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATS 420

Query: 423 EAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIV 482
           EAF  GW  +GD+GV HPDGY+E+KDRSKDIIISGGENISSIEVE VLY +  +LE A+V
Sbjct: 421 EAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVV 480

Query: 483 GRPDDHWGETPCAFVKLK---DGCSANGEEIIKFCREHLPHYMAPRSVV-FRDLPKTSTG 542
             P   WGETPCAFV LK   +G   +  ++IK+CRE++PH+M P+ VV F++LPK S G
Sbjct: 481 AMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNG 540

Query: 543 KIQKFVLKEEATAM 548
           KI K  L++ A A+
Sbjct: 541 KILKSKLRDIAKAL 548