MC08g2387.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC08g2387.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	E3 ubiquitin-protein ligase EL5
Location	MC08: 32654799 .. 32657569 (-)
Sequence length	1860
RNA-Seq Expression	MC08g2387.1
Synteny	MC08g2387.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTATGTAAAATTCAAGAAACTAATATAAAGTTTAAAAAAGTTGAGGAATAAATATATAATTTAAGGAATTAGCTAAAATAGAATAAAGACTGAAAATAAAAACAACCCATCATTGTCTTTAGTTTTGGTGGGTTCTGTGAGGTAATGATCAATTTATCACATTATGAGGCACCTGTAAATTTTGCGGGAACCTTCTTCACTCCTTCCTCTCCTCCTTTTTCTCTCACTAATTTTTTTAGAGAGAGAGAATGATTGAGGAAATCGCATTGTACGTTTTACTACTCCCACCTTCAATGGCGGGGACCCGCTTTTGTCTCTTACCTTCATCACTCCCCATTTATACTTTATCCTTATTTTAATTTCCATTTCAGAAGTTTTTCTCCATTTTGTTCTTCACTTCTTTGTGTTCCCCGTTTTTATGTTTCTGGGTTTCTTATTGATTTGCCGGAAATGGGAGATTTCGCCGATTCTGAGCGGTAATCGTTGCCGGGTTTGTTTTCTCTGTTTCTGGTTTAAATTTTGATGAAACTGAGAAACTGTATTGATTTTGAGTTGAAATCGGAGTGGGTTTGGGACATTTTTGCGAGTTCTCTCTGTTTCTTCTCTCTCTCTGTGTTCTTCGTCGTTTTTCTGCTACTGGTTTCTGATTCATGAACCGAGAAACTCCATCGATTCTGAATTGAAATCGGTTTGGGTTTCGGAATTTTAGCAAGTTTTTTCTCTCTATTCTTATTCGTCTTCCTCACTTTTCTGTAACTGGGCTTCTGATTTTGAGTCGAAATCGTCATGGGTTTTGAGTATTAATTGGAGCAAGTTTTTCCCATTTCTCTCTCTGATCTTCATTTTTCTCACTTTTCTATAACTGGGTTCTGATTCTTTTTCTGAAACTGGGAAACTCGATGAATTTTGAGTTGAAATCTTCGTGGGTTTGATTCTCAATTCCAGAAAGTTCTCACCATTTCCCTCTCTGTTCTTCCATTAATCTGCCGAAACTGGAAAGTTTTGCCCATTTTCCGCTCTAATCTTCTTCATCTTCCTCACTTTTCCGCAACTCGGTTCGAATTAAATCATCCAAACCCAAGCAATTTCGTTGATTTGTTTCCTCCGATCAATGGCGGGAATGTTGCCGGGAGTCGAATGCGCGAGAAGGCGGCGGTTCCACCAGAGCATAACCTCGTCGGACTCGCCGTCCACGGCGGCGAAACTGGGATCCACCAGAAGGCCCTCCTTCTGTTTGTACACCAGCAACCGCGATTTCCACCTCACTTCATCTTCTACACAGGTAAAGAAAAAAAATCTACATACAATTAAATTCCATCCACATCCTTGTTCCAATTATCTGTTTGTTTCCTGAGAAAAATCTGGTGAATGATTTTCAAATTTAAACAAAAATTAAGCAACGGAGCTGGTCGAATCAAGCTTTCCAGAACGATAAACTGGGTGAAATTGCGAGAGAAGCCAAAGAGAGGTTGGATGAGAGGTTGAAGACACGCAGAAGACCAGAGACTGAAACTGTAAGGTAATTTCTCCTCGGTGAGCTATTTTTCTCTCTCTCTCTTTTTTGTTTCATTGAGAATTTTATCGATTATCATTAAAAATTAAAAGCGTCTCGCGGATAATAAATAATCTCTATGCTTATGCTTAATATCATAAATAATAGTTACATGTATAAAAAGATTTAAGTACCATTAATTTTTTAAAATAATCATCCAAGTGTTAATTGAATGATTAATGAGAATGTGAGTAATTTTTACGGTTTTGGTCTATGTCAAAATCACTCTCAAAGACACCCTGAGTAAAATTGCACAATTATTTCACAAAATTTTGTAAGGTTTGTCTTTTATTTGGGTATTTAGTCAACTATATTAATATGTTAGGTTGAGTAAAAATTAAAAGGACTATAAGACTAAACTTGTAATTTACTTCAAATATTTTAGGTTAAGCTATTAGTTTAGTCTCTAAATTTTCAAGATTGTTTTTAATGCGTCCTTGAATTTAAAAAAAAAAAATCTAATAAGTTGGTAGATCTTCAACTTTGTGTCAAATACTTATTAGACATAAAACTAAAAGAATGATATTAAGGTATCAATAAGTAGGTATTTCCTGATACTAGAAGAGTACAAATACTTTTTTCGTTTATTGTCAACCATGTTAGTATTGTATTAAAAATAATGTAAGGTCCGGTAACAAATAAAATCATAGTGTGAATAGAGAGAATATAGCACCTCATAGTACTATCTGTTAAAGCACCTAAGCTTAGGTCTGGATTTGATGGAATTTTGAAAGTTTTATAACTTGATATGGTACAATTATAGGAAGAGTAGGAGTAAGAGGGAAGAAGAAGTAAGGTCAAAAGGGTCAGAAAAGGAAGGGGAAATGACAAAAGGCAAAGAAGAAGAGAGAAAGAAGAAGTTGATGAGTTGGGGAAAGTGGAAGGCGTGGGAGCAAAAGGAGTGCGCCGTTTGCCTAGAAGGGTTCAGGGGTGGCGAGCCGGTGGTCCATCTGCCATGCGCCCACAAGTTCCATTCCTCCTGCCTCGTCCCTTGGCTCCAAACCAACTCCCATTGCCCTTCTTGCAGATTCCCCATTTCCGCTCCTTAATATTTATTTCACTCTTTTTTTTCCCTTCTTTAGTTTTAGTTTTAGTAATCTCTTTGTTTTATTTCGGTCTTTTTTTGTATCTAAACTTTTGAGTATTTTACCACGGTCGAATTTTAATACTTCTTCTTGATTGGTTACGTAAGTATTTTCTATTGACTATCTTTGTG

mRNA sequence

ATTTTATGTAAAATTCAAGAAACTAATATAAAGTTTAAAAAAGTTGAGGAATAAATATATAATTTAAGGAATTAGCTAAAATAGAATAAAGACTGAAAATAAAAACAACCCATCATTGTCTTTAGTTTTGGTGGGTTCTGTGAGGTAATGATCAATTTATCACATTATGAGGCACCTGTAAATTTTGCGGGAACCTTCTTCACTCCTTCCTCTCCTCCTTTTTCTCTCACTAATTTTTTTAGAGAGAGAGAATGATTGAGGAAATCGCATTGTACGTTTTACTACTCCCACCTTCAATGGCGGGGACCCGCTTTTGTCTCTTACCTTCATCACTCCCCATTTATACTTTATCCTTATTTTAATTTCCATTTCAGAAGTTTTTCTCCATTTTGTTCTTCACTTCTTTGTGTTCCCCGTTTTTATGTTTCTGGGTTTCTTATTGATTTGCCGGAAATGGGAGATTTCGCCGATTCTGAGCGGTAATCGTTGCCGGGTTTGTTTTCTCTGTTTCTGGTTTAAATTTTGATGAAACTGAGAAACTGTATTGATTTTGAGTTGAAATCGGAGTGGGTTTGGGACATTTTTGCGAGTTCTCTCTGTTTCTTCTCTCTCTCTGTGTTCTTCGTCGTTTTTCTGCTACTGGTTTCTGATTCATGAACCGAGAAACTCCATCGATTCTGAATTGAAATCGGTTTGGGTTTCGGAATTTTAGCAAGTTTTTTCTCTCTATTCTTATTCGTCTTCCTCACTTTTCTGTAACTGGGCTTCTGATTTTGAGTCGAAATCGTCATGGGTTTTGAGTATTAATTGGAGCAAGTTTTTCCCATTTCTCTCTCTGATCTTCATTTTTCTCACTTTTCTATAACTGGGTTCTGATTCTTTTTCTGAAACTGGGAAACTCGATGAATTTTGAGTTGAAATCTTCGTGGGTTTGATTCTCAATTCCAGAAAGTTCTCACCATTTCCCTCTCTGTTCTTCCATTAATCTGCCGAAACTGGAAAGTTTTGCCCATTTTCCGCTCTAATCTTCTTCATCTTCCTCACTTTTCCGCAACTCGGTTCGAATTAAATCATCCAAACCCAAGCAATTTCGTTGATTTGTTTCCTCCGATCAATGGCGGGAATGTTGCCGGGAGTCGAATGCGCGAGAAGGCGGCGGTTCCACCAGAGCATAACCTCGTCGGACTCGCCGTCCACGGCGGCGAAACTGGGATCCACCAGAAGGCCCTCCTTCTGTTTGTACACCAGCAACCGCGATTTCCACCTCACTTCATCTTCTACACAGCAACGGAGCTGGTCGAATCAAGCTTTCCAGAACGATAAACTGGGTGAAATTGCGAGAGAAGCCAAAGAGAGGTTGGATGAGAGGTTGAAGACACGCAGAAGACCAGAGACTGAAACTGTAAGGAAGAGTAGGAGTAAGAGGGAAGAAGAAGTAAGGTCAAAAGGGTCAGAAAAGGAAGGGGAAATGACAAAAGGCAAAGAAGAAGAGAGAAAGAAGAAGTTGATGAGTTGGGGAAAGTGGAAGGCGTGGGAGCAAAAGGAGTGCGCCGTTTGCCTAGAAGGGTTCAGGGGTGGCGAGCCGGTGGTCCATCTGCCATGCGCCCACAAGTTCCATTCCTCCTGCCTCGTCCCTTGGCTCCAAACCAACTCCCATTGCCCTTCTTGCAGATTCCCCATTTCCGCTCCTTAATATTTATTTCACTCTTTTTTTTCCCTTCTTTAGTTTTAGTTTTAGTAATCTCTTTGTTTTATTTCGGTCTTTTTTTGTATCTAAACTTTTGAGTATTTTACCACGGTCGAATTTTAATACTTCTTCTTGATTGGTTACGTAAGTATTTTCTATTGACTATCTTTGTG

Coding sequence (CDS)

ATGGCGGGAATGTTGCCGGGAGTCGAATGCGCGAGAAGGCGGCGGTTCCACCAGAGCATAACCTCGTCGGACTCGCCGTCCACGGCGGCGAAACTGGGATCCACCAGAAGGCCCTCCTTCTGTTTGTACACCAGCAACCGCGATTTCCACCTCACTTCATCTTCTACACAGCAACGGAGCTGGTCGAATCAAGCTTTCCAGAACGATAAACTGGGTGAAATTGCGAGAGAAGCCAAAGAGAGGTTGGATGAGAGGTTGAAGACACGCAGAAGACCAGAGACTGAAACTGTAAGGAAGAGTAGGAGTAAGAGGGAAGAAGAAGTAAGGTCAAAAGGGTCAGAAAAGGAAGGGGAAATGACAAAAGGCAAAGAAGAAGAGAGAAAGAAGAAGTTGATGAGTTGGGGAAAGTGGAAGGCGTGGGAGCAAAAGGAGTGCGCCGTTTGCCTAGAAGGGTTCAGGGGTGGCGAGCCGGTGGTCCATCTGCCATGCGCCCACAAGTTCCATTCCTCCTGCCTCGTCCCTTGGCTCCAAACCAACTCCCATTGCCCTTCTTGCAGATTCCCCATTTCCGCTCCTTAA

Protein sequence

MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSSTQQRSWSNQAFQNDKLGEIAREAKERLDERLKTRRRPETETVRKSRSKREEEVRSKGSEKEGEMTKGKEEERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSHCPSCRFPISAP

Homology

[Close]

BLAST of MC08g2387.1 vs. ExPASy Swiss-Prot
Match: Q852U6 (Probable E3 ubiquitin-protein ligase RHY1A OS=Arabidopsis thaliana OX=3702 GN=RHY1A PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.0e-10
Identity = 25/50 (50.00%), Postives = 34/50 (68.00%), Query Frame = 0


Query: 141 EQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSHCPSCRFPIS 191
           E ++C++CLE F  G+ ++ LPC H FHSSCL PWL+    CP CR  I+
Sbjct: 199 EMRDCSICLESFTKGDMLISLPCTHSFHSSCLNPWLRACGDCPCCRRAIA 248

[Close]

BLAST of MC08g2387.1 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 5.9e-10
Identity = 23/49 (46.94%), Postives = 32/49 (65.31%), Query Frame = 0


Query: 141 EQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSHCPSCRFPI 190
           E  +CAVC++ F  G  V  +PC H FH  CL+PWL+ ++ CP CRF +
Sbjct: 212 EMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFEL 260

[Close]

BLAST of MC08g2387.1 vs. ExPASy Swiss-Prot
Match: Q9H9V4 (RING finger protein 122 OS=Homo sapiens OX=9606 GN=RNF122 PE=1 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 2.9e-09
Identity = 29/64 (45.31%), Postives = 37/64 (57.81%), Query Frame = 0


Query: 129 KKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSHCPSCRFP 188
           KKL  +G       + CAVCLE F+G + +  LPC H FH  CLV WL+    CP C  P
Sbjct: 84  KKLQLYG-------QTCAVCLEDFKGKDELGVLPCQHAFHRKCLVKWLEVRCVCPMCNKP 140

Query: 189 ISAP 193
           I++P
Sbjct: 144 IASP 140

[Close]

BLAST of MC08g2387.1 vs. ExPASy Swiss-Prot
Match: Q8BP31 (RING finger protein 122 OS=Mus musculus OX=10090 GN=Rnf122 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.8e-09
Identity = 29/64 (45.31%), Postives = 36/64 (56.25%), Query Frame = 0


Query: 129 KKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSHCPSCRFP 188
           KKL  +G       + CAVCLE F+G + +  LPC H FH  CLV WL+    CP C  P
Sbjct: 84  KKLQLYG-------QTCAVCLEDFKGKDELGVLPCQHAFHRKCLVKWLEVRCVCPMCNKP 140

Query: 189 ISAP 193
           I+ P
Sbjct: 144 IAGP 140

[Close]

BLAST of MC08g2387.1 vs. ExPASy Swiss-Prot
Match: Q91YL2 (E3 ubiquitin-protein ligase RNF126 OS=Mus musculus OX=10090 GN=Rnf126 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-08
Identity = 24/43 (55.81%), Postives = 28/43 (65.12%), Query Frame = 0


Query: 144 ECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSHCPSCR 187
           EC VC E +  GE V  LPC H FH SC+VPWL+ +  CP CR
Sbjct: 230 ECPVCKEDYALGESVRQLPCNHLFHDSCIVPWLEQHDSCPVCR 272

[Close]

BLAST of MC08g2387.1 vs. NCBI nr
Match: XP_022143236.1 (E3 ubiquitin-protein ligase EL5 [Momordica charantia])

HSP 1 Score: 378 bits (971), Expect = 3.45e-132
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSSTQQRS 60
           MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSSTQQRS
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSSTQQRS 60

Query: 61  WSNQAFQNDKLGEIAREAKERLDERLKTRRRPETETVRKSRSKREEEVRSKGSEKEGEMT 120
           WSNQAFQNDKLGEIAREAKERLDERLKTRRRPETETVRKSRSKREEEVRSKGSEKEGEMT
Sbjct: 61  WSNQAFQNDKLGEIAREAKERLDERLKTRRRPETETVRKSRSKREEEVRSKGSEKEGEMT 120

Query: 121 KGKEEERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNS 180
           KGKEEERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNS
Sbjct: 121 KGKEEERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNS 180

Query: 181 HCPSCRFPISAP 192
           HCPSCRFPISAP
Sbjct: 181 HCPSCRFPISAP 192

[Close]

BLAST of MC08g2387.1 vs. NCBI nr
Match: KAG7030344.1 (putative E3 ubiquitin-protein ligase RHY1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 262 bits (670), Expect = 2.27e-86
Identity = 140/193 (72.54%), Postives = 156/193 (80.83%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSST--QQ 60
           MAGMLPGVECARRRRFHQSIT SD+PSTAAKLGSTRRPSFCLYTSNRDFH+TSSS+  QQ
Sbjct: 1   MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60

Query: 61  RSWSNQAFQNDKLGEIAREAKERLDERLKTRRRPETETVRKSRSKREEEVRSKGSEKEGE 120
           RS S+QA+QN+KLGEIAREAKERLDERL+T R+PE    +            K S ++ E
Sbjct: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPRKKSGEG-------LKNSLEKKE 120

Query: 121 MTKGKEEERKKKLMSWGK---WKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPW 180
             KGKEE   KK  +WGK   WKA EQ+ECAVCLEGFR GEP+VHLPCAHKFHS+CLVPW
Sbjct: 121 KAKGKEESITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPW 180

Query: 181 LQTNSHCPSCRFP 188
           LQTN+HCP CRFP
Sbjct: 181 LQTNAHCPCCRFP 186

[Close]

BLAST of MC08g2387.1 vs. NCBI nr
Match: XP_004139268.1 (probable E3 ubiquitin-protein ligase RHY1A [Cucumis sativus] >KGN60748.1 hypothetical protein Csa_019469 [Cucumis sativus])

HSP 1 Score: 259 bits (663), Expect = 3.96e-85
Identity = 140/200 (70.00%), Postives = 162/200 (81.00%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHL-TSSSTQQR 60
           MAGMLPGVECARRRRFHQSI SSDSP TA K GSTRRPSFCLYTSNRDFHL +SSS+QQR
Sbjct: 1   MAGMLPGVECARRRRFHQSINSSDSPFTAGKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60

Query: 61  SWSNQAFQNDKLGEIAREAKERLDERLKTRRRPETETVRKSRSK---REEEVRSKGSEKE 120
           S +NQA+QNDKLGEIAREAKERLDERL+T R+PE    + +  +   +E   ++ G +KE
Sbjct: 61  SLTNQAYQNDKLGEIAREAKERLDERLRTHRKPENSRKKNNNEEVKGKESREKNNGEKKE 120

Query: 121 GEMTKGKEEERKKKLMSWG----KWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCL 180
            + TKGKEEE KK+  SWG    KWKA EQ+EC+VCLE FR  EP++HLPCAHKFHS+CL
Sbjct: 121 MKNTKGKEEETKKRF-SWGRKLMKWKASEQEECSVCLERFRLSEPLLHLPCAHKFHSTCL 180

Query: 181 VPWLQTNSHCPSCRFPISAP 192
           VPWLQ N+HCP CRFPI+ P
Sbjct: 181 VPWLQANAHCPCCRFPIAVP 199

[Close]

BLAST of MC08g2387.1 vs. NCBI nr
Match: XP_022946687.1 (probable E3 ubiquitin-protein ligase RHY1A [Cucurbita moschata])

HSP 1 Score: 256 bits (655), Expect = 2.90e-84
Identity = 139/193 (72.02%), Postives = 153/193 (79.27%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSST--QQ 60
           MAGMLPGVECARRRRFHQSITSSD+PSTAAKLGSTRRPSFCLYTSNRDFH+TSSS+  QQ
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60

Query: 61  RSWSNQAFQNDKLGEIAREAKERLDERLKTRRRPETETVRKSRSKREEEVRSKGSEKEGE 120
           RS S+QA+QN+KLGEIAREAKERLDERL+T R+PE                    +K GE
Sbjct: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPR-----------------KKSGE 120

Query: 121 MTKGKEEERKKKLMSWGK---WKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPW 180
             K  EE   KK  +WGK   WKA EQ+ECAVCLEGFR GEP+VHLPCAHKFHS+CLVPW
Sbjct: 121 GWK--EESITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPW 174

Query: 181 LQTNSHCPSCRFP 188
           LQTN+HCP CRFP
Sbjct: 181 LQTNAHCPCCRFP 174

[Close]

BLAST of MC08g2387.1 vs. NCBI nr
Match: XP_023545449.1 (probable E3 ubiquitin-protein ligase RHY1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 256 bits (655), Expect = 3.00e-84
Identity = 138/194 (71.13%), Postives = 152/194 (78.35%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSST---Q 60
           MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFH+TSSS+   Q
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSSSSQ 60

Query: 61  QRSWSNQAFQNDKLGEIAREAKERLDERLKTRRRPETETVRKSRSKREEEVRSKGSEKEG 120
           QRS SNQA+QN+KLGEIAREAKERLDERL+T R+PE                    +K G
Sbjct: 61  QRSSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENPR-----------------KKSG 120

Query: 121 EMTKGKEEERKKKLMSWGK---WKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVP 180
           E +K  EE   KK  +WGK   WKA EQ+EC +CLEGFR GEP+VHLPCAHKFHS+CLVP
Sbjct: 121 EGSK--EESITKKRFTWGKLMKWKASEQEECTICLEGFRAGEPLVHLPCAHKFHSACLVP 175

Query: 181 WLQTNSHCPSCRFP 188
           WLQ N+HCP CRFP
Sbjct: 181 WLQANAHCPCCRFP 175

[Close]

BLAST of MC08g2387.1 vs. ExPASy TrEMBL
Match: A0A6J1CPN1 (E3 ubiquitin-protein ligase EL5 OS=Momordica charantia OX=3673 GN=LOC111013152 PE=4 SV=1)

HSP 1 Score: 378 bits (971), Expect = 1.67e-132
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSSTQQRS 60
           MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSSTQQRS
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSSTQQRS 60

Query: 61  WSNQAFQNDKLGEIAREAKERLDERLKTRRRPETETVRKSRSKREEEVRSKGSEKEGEMT 120
           WSNQAFQNDKLGEIAREAKERLDERLKTRRRPETETVRKSRSKREEEVRSKGSEKEGEMT
Sbjct: 61  WSNQAFQNDKLGEIAREAKERLDERLKTRRRPETETVRKSRSKREEEVRSKGSEKEGEMT 120

Query: 121 KGKEEERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNS 180
           KGKEEERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNS
Sbjct: 121 KGKEEERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNS 180

Query: 181 HCPSCRFPISAP 192
           HCPSCRFPISAP
Sbjct: 181 HCPSCRFPISAP 192

[Close]

BLAST of MC08g2387.1 vs. ExPASy TrEMBL
Match: A0A0A0LG47 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009360 PE=4 SV=1)

HSP 1 Score: 259 bits (663), Expect = 1.92e-85
Identity = 140/200 (70.00%), Postives = 162/200 (81.00%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHL-TSSSTQQR 60
           MAGMLPGVECARRRRFHQSI SSDSP TA K GSTRRPSFCLYTSNRDFHL +SSS+QQR
Sbjct: 1   MAGMLPGVECARRRRFHQSINSSDSPFTAGKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60

Query: 61  SWSNQAFQNDKLGEIAREAKERLDERLKTRRRPETETVRKSRSK---REEEVRSKGSEKE 120
           S +NQA+QNDKLGEIAREAKERLDERL+T R+PE    + +  +   +E   ++ G +KE
Sbjct: 61  SLTNQAYQNDKLGEIAREAKERLDERLRTHRKPENSRKKNNNEEVKGKESREKNNGEKKE 120

Query: 121 GEMTKGKEEERKKKLMSWG----KWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCL 180
            + TKGKEEE KK+  SWG    KWKA EQ+EC+VCLE FR  EP++HLPCAHKFHS+CL
Sbjct: 121 MKNTKGKEEETKKRF-SWGRKLMKWKASEQEECSVCLERFRLSEPLLHLPCAHKFHSTCL 180

Query: 181 VPWLQTNSHCPSCRFPISAP 192
           VPWLQ N+HCP CRFPI+ P
Sbjct: 181 VPWLQANAHCPCCRFPIAVP 199

[Close]

BLAST of MC08g2387.1 vs. ExPASy TrEMBL
Match: A0A6J1G4D6 (probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita moschata OX=3662 GN=LOC111450678 PE=4 SV=1)

HSP 1 Score: 256 bits (655), Expect = 1.41e-84
Identity = 139/193 (72.02%), Postives = 153/193 (79.27%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSST--QQ 60
           MAGMLPGVECARRRRFHQSITSSD+PSTAAKLGSTRRPSFCLYTSNRDFH+TSSS+  QQ
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60

Query: 61  RSWSNQAFQNDKLGEIAREAKERLDERLKTRRRPETETVRKSRSKREEEVRSKGSEKEGE 120
           RS S+QA+QN+KLGEIAREAKERLDERL+T R+PE                    +K GE
Sbjct: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPR-----------------KKSGE 120

Query: 121 MTKGKEEERKKKLMSWGK---WKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPW 180
             K  EE   KK  +WGK   WKA EQ+ECAVCLEGFR GEP+VHLPCAHKFHS+CLVPW
Sbjct: 121 GWK--EESITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPW 174

Query: 181 LQTNSHCPSCRFP 188
           LQTN+HCP CRFP
Sbjct: 181 LQTNAHCPCCRFP 174

[Close]

BLAST of MC08g2387.1 vs. ExPASy TrEMBL
Match: A0A6J1HQI5 (probable E3 ubiquitin-protein ligase RHY1A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465688 PE=4 SV=1)

HSP 1 Score: 255 bits (652), Expect = 5.45e-84
Identity = 139/194 (71.65%), Postives = 151/194 (77.84%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSSTQQRS 60
           MAGMLPGVECARRRRFHQSITSS+S STAAKLGSTRR SFCLYTSNRDFHLTSSS+QQR 
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC 60

Query: 61  WSNQAFQNDKLGEIAREAKERLDERLKTRRRPETETVRKSRSKREEEVRSKGSEKEGEMT 120
            SN A+QN+KLG+ AREAKERLDERL+T R+PE    + S     EEVR K      E  
Sbjct: 61  SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSS----SEEVRLKK-----ENV 120

Query: 121 KGKEEERKKKLMSWGK---WKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQ 180
           KGKEE   KK  SWGK   WKA EQ+EC VCLE FR  EP+VHLPCAHKFHS CLVPWLQ
Sbjct: 121 KGKEETTTKKRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQ 180

Query: 181 TNSHCPSCRFPISA 191
            N+HCP CR+PI A
Sbjct: 181 ANAHCPCCRYPILA 185

[Close]

BLAST of MC08g2387.1 vs. ExPASy TrEMBL
Match: A0A1S3C563 (RING-H2 finger protein ATL16 OS=Cucumis melo OX=3656 GN=LOC103496570 PE=4 SV=1)

HSP 1 Score: 251 bits (641), Expect = 3.73e-82
Identity = 136/195 (69.74%), Postives = 157/195 (80.51%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHL-TSSSTQQR 60
           MAGMLPGVECARRRRFH SI SSDSPSTAAK GSTRRPSFCLYTSNRDFHL +SSS+QQR
Sbjct: 1   MAGMLPGVECARRRRFHHSINSSDSPSTAAKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60

Query: 61  SWSNQAFQNDKLGEIAREAKERLDERLKTRRRPETETVRKSRSK----REEEVRSKGSEK 120
           S +N+A QN+KLGEIAREAKERLDERL+T R+PE    + S+++    +E   ++ G  K
Sbjct: 61  SLTNKAHQNEKLGEIAREAKERLDERLRTHRKPENSRSKNSKNEEVKWKESREKNNGERK 120

Query: 121 EGEMTKGKEEERKKKLMSWG----KWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSC 180
           E   TKGKEEE +K+  SWG    KWKA EQ EC+VCLE FR GEP++HLPCAHKFHS+C
Sbjct: 121 EMRSTKGKEEETRKRF-SWGRKLMKWKASEQDECSVCLERFRSGEPLLHLPCAHKFHSTC 180

Query: 181 LVPWLQTNSHCPSCR 186
           LVPWLQ N+HCP CR
Sbjct: 181 LVPWLQANAHCPCCR 194

[Close]

BLAST of MC08g2387.1 vs. TAIR 10
Match: AT2G15580.1 (RING/U-box superfamily protein )

HSP 1 Score: 144.1 bits (362), Expect = 1.2e-34
Identity = 94/197 (47.72%), Postives = 122/197 (61.93%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFH---QSITSSDSPSTAAKLGS--TRRPSFCLYTSNRDFHLTSSS 60
           MAGMLPGVECARRRRFH     I SS++ S AA  G   TRRPSF LYT+N + H    S
Sbjct: 1   MAGMLPGVECARRRRFHGGAPPIESSNTASVAAAAGHVWTRRPSFSLYTTNHESHQAHVS 60

Query: 61  TQQRSWSNQAFQND---KLGEIAREAKERLDERLKTRRRPETETVRKSRSKREEEVRSKG 120
             +RS  N+++  D   KL   A+EAK+RL++RL  R  P T    K +  + E+ + K 
Sbjct: 61  FSERSVRNKSYGEDNDEKLDGAAKEAKQRLNKRL--RIPPRTRQNGKDKGNKLEQGKGKP 120

Query: 121 SEKEGEMTKGKEEERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCL 180
                    G ++ R  +LM W K +  EQ++CA+CL+ F+ GE +VHLPCAHKFHS CL
Sbjct: 121 LGDLPTEVVGLKKSR-GRLMEWFKRRVREQQDCAICLDRFKKGETLVHLPCAHKFHSICL 180

Query: 181 VPWLQTNSHCPSCRFPI 190
           +PWL TN +CP CR  I
Sbjct: 181 LPWLDTNVYCPYCRTDI 194

[Close]

BLAST of MC08g2387.1 vs. TAIR 10
Match: AT2G15580.2 (RING/U-box superfamily protein )

HSP 1 Score: 74.7 bits (182), Expect = 9.0e-14
Identity = 52/109 (47.71%), Postives = 69/109 (63.30%), Query Frame = 0


Query: 1   MAGMLPGVECARRRRFH---QSITSSDSPSTAAKLGS--TRRPSFCLYTSNRDFHLTSSS 60
           MAGMLPGVECARRRRFH     I SS++ S AA  G   TRRPSF LYT+N + H    S
Sbjct: 1   MAGMLPGVECARRRRFHGGAPPIESSNTASVAAAAGHVWTRRPSFSLYTTNHESHQAHVS 60

Query: 61  TQQRSWSNQAFQND---KLGEIAREAKERLDERLKTRRRPETETVRKSR 102
             +RS  N+++  D   KL   A+EAK+RL++RL+   R  +  + K++
Sbjct: 61  FSERSVRNKSYGEDNDEKLDGAAKEAKQRLNKRLRIPPRTSSGKMVKTK 109

[Close]

BLAST of MC08g2387.1 vs. TAIR 10
Match: AT1G49850.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.4 bits (163), Expect = 1.4e-11
Identity = 25/50 (50.00%), Postives = 34/50 (68.00%), Query Frame = 0


Query: 141 EQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSHCPSCRFPIS 191
           E ++C++CLE F  G+ ++ LPC H FHSSCL PWL+    CP CR  I+
Sbjct: 199 EMRDCSICLESFTKGDMLISLPCTHSFHSSCLNPWLRACGDCPCCRRAIA 248

[Close]

BLAST of MC08g2387.1 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.9 bits (159), Expect = 4.2e-11
Identity = 23/49 (46.94%), Postives = 32/49 (65.31%), Query Frame = 0


Query: 141 EQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSHCPSCRFPI 190
           E  +CAVC++ F  G  V  +PC H FH  CL+PWL+ ++ CP CRF +
Sbjct: 212 EMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFEL 260

[Close]

BLAST of MC08g2387.1 vs. TAIR 10
Match: AT3G58720.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.1 bits (157), Expect = 7.1e-11
Identity = 31/71 (43.66%), Postives = 39/71 (54.93%), Query Frame = 0


Query: 122 GKEEERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSH 181
           G E  R  + +S  +    + K C+VCLE F   E V+  PC H FH  C+VPWL+T   
Sbjct: 125 GSESSRNTQNLSGEE----DDKRCSVCLEDFEPKETVMLTPCKHMFHEECIVPWLKTKGQ 184

Query: 182 CPSCRFPISAP 193
           CP CRF I  P
Sbjct: 185 CPVCRFVILKP 191

The following BLAST results are available for this feature:

BLAST of MC08g2387.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q852U6	2.0e-10	50.00	Probable E3 ubiquitin-protein ligase RHY1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q8LPN7	5.9e-10	46.94	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q9H9V4	2.9e-09	45.31	RING finger protein 122 OS=Homo sapiens OX=9606 GN=RNF122 PE=1 SV=2	[more]
Q8BP31	3.8e-09	45.31	RING finger protein 122 OS=Mus musculus OX=10090 GN=Rnf122 PE=2 SV=1	[more]
Q91YL2	1.1e-08	55.81	E3 ubiquitin-protein ligase RNF126 OS=Mus musculus OX=10090 GN=Rnf126 PE=1 SV=1	[more]

BLAST of MC08g2387.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143236.1	3.45e-132	100.00	E3 ubiquitin-protein ligase EL5 [Momordica charantia]	[more]
KAG7030344.1	2.27e-86	72.54	putative E3 ubiquitin-protein ligase RHY1A, partial [Cucurbita argyrosperma subs...	[more]
XP_004139268.1	3.96e-85	70.00	probable E3 ubiquitin-protein ligase RHY1A [Cucumis sativus] >KGN60748.1 hypothe...	[more]
XP_022946687.1	2.90e-84	72.02	probable E3 ubiquitin-protein ligase RHY1A [Cucurbita moschata]	[more]
XP_023545449.1	3.00e-84	71.13	probable E3 ubiquitin-protein ligase RHY1A [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC08g2387.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CPN1	1.67e-132	100.00	E3 ubiquitin-protein ligase EL5 OS=Momordica charantia OX=3673 GN=LOC111013152 P...	[more]
A0A0A0LG47	1.92e-85	70.00	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009360 P...	[more]
A0A6J1G4D6	1.41e-84	72.02	probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1HQI5	5.45e-84	71.65	probable E3 ubiquitin-protein ligase RHY1A isoform X1 OS=Cucurbita maxima OX=366...	[more]
A0A1S3C563	3.73e-82	69.74	RING-H2 finger protein ATL16 OS=Cucumis melo OX=3656 GN=LOC103496570 PE=4 SV=1	[more]

BLAST of MC08g2387.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G15580.1	1.2e-34	47.72	RING/U-box superfamily protein	[more]
AT2G15580.2	9.0e-14	47.71	RING/U-box superfamily protein	[more]
AT1G49850.1	1.4e-11	50.00	RING/U-box superfamily protein	[more]
AT3G19950.1	4.2e-11	46.94	RING/U-box superfamily protein	[more]
AT3G58720.1	7.1e-11	43.66	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 145..185 e-value: 1.5E-6 score: 37.8
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 143..186 e-value: 8.3E-13 score: 48.5
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 145..186 score: 12.504031
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 118..191 e-value: 2.2E-18 score: 67.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 20..37
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 16..37
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 82..128
None	No IPR available	PANTHER	PTHR22765:SF204	RING/U-BOX SUPERFAMILY PROTEIN	coord: 1..188
None	No IPR available	PANTHER	PTHR22765	RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING	coord: 1..188
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 144..186 e-value: 4.81316E-18 score: 71.9493
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 132..191

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC08g2387	MC08g2387	gene

The following utr3 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC08g2387.1-utr3	MC08g2387.1-utr3-MC08:32654799..32654965	utr3

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC08g2387.1-cds	MC08g2387.1-cds-MC08:32654966..32655251	CDS
MC08g2387.1-cds	MC08g2387.1-cds-MC08:32656048..32656169	CDS
MC08g2387.1-cds	MC08g2387.1-cds-MC08:32656285..32656455	CDS

The following utr5 feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC08g2387.1-utr5	MC08g2387.1-utr5-MC08:32656456..32657569	utr5

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC08g2387.1	MC08g2387.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
molecular_function	GO:0061630	ubiquitin protein ligase activity