MC08g2325.1 (mRNA) Bitter gourd (Dali-11) v1

BLAST of MC08g2325.1 vs. ExPASy Swiss-Prot
Match: Q9LY77 (Calcium-transporting ATPase 12, plasma membrane-type OS=Arabidopsis thaliana OX=3702 GN=ACA12 PE=2 SV=1)

HSP 1 Score: 1219.9 bits (3155), Expect = 0.0e+00
Identity = 635/1007 (63.06%), Postives = 789/1007 (78.35%), Query Frame = 0

Query: 18   SSMAKNHKRRWRMAFAAICFTRVLISLSKKVGGRR---KSGGLLRSLSYVAVDVPLNDGD 77
            +S     +RRWR A+AAI   R ++SL K++   R   K+     SLSY A++    +G 
Sbjct: 18   TSSLNKAQRRWRFAYAAIYSMRAMLSLVKEIVPARIDPKTSDASLSLSYTALE--SGEGA 77

Query: 78   STDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGLAALLETSANGGISATDDD 137
              +   LS + A   +D+E L E+++ KD   +   GGVEG+AA L T+   GI   + +
Sbjct: 78   KINSMPLSYVPA---IDQEQLVEIMKGKDLPGIQALGGVEGVAASLRTNPTKGIHGNEQE 137

Query: 138  LIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAALSLGFGIKQHGWDDGWYD 197
            +  RR+ FG+NTY K   +  L FV EAFKD TI+ILL+CA  SLGFGIK+HG  +GWY+
Sbjct: 138  VSRRRDLFGSNTYHKPPPKGLLFFVYEAFKDLTILILLVCAIFSLGFGIKEHGIKEGWYE 197

Query: 198  GGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRAGRRKPVSIFEVVVGDVV 257
            GGSI +A+FLV+VVSA+SNF Q RQF+KLS   ++IK++V+R  RR+ +SIF+VVVGDVV
Sbjct: 198  GGSIFVAVFLVIVVSALSNFRQERQFDKLSKISNNIKVEVLRDSRRQHISIFDVVVGDVV 257

Query: 258  RLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPFLLSGTKVSDGFGVMMVT 317
             LKIGDQ+PADG+FLEGHSL+VDESSM GES+H+EV+   NPFL SGTK+ DGF  M+V 
Sbjct: 258  FLKIGDQIPADGLFLEGHSLQVDESSMTGESDHLEVDHKDNPFLFSGTKIVDGFAQMLVV 317

Query: 318  SVGMNTAWGEMMSSIREEVNELTPLQACLNKMTAVVGKLGLTVAVLVLLVLLLRYFTGST 377
            SVGM+T WG+ MSSI ++ +E TPLQ  L+ +T+ +GK+GLTVA LVL+VLL+RYFTG+T
Sbjct: 318  SVGMSTTWGQTMSSINQDSSERTPLQVRLDTLTSTIGKIGLTVAALVLVVLLVRYFTGNT 377

Query: 378  -----KEFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLAYSMKKMMADN 437
                 +E+NGSKT  ++V+N+V+ +VAAAVTIVVVAIPEGLPLAVTLTLAYSMK+MM+D 
Sbjct: 378  EKEGKREYNGSKTPVDTVVNSVVRIVAAAVTIVVVAIPEGLPLAVTLTLAYSMKRMMSDQ 437

Query: 438  AMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDRYSSSTIEAKVVEVLH 497
            AMVRKLSACETMGSAT ICTDKTGTLTLNEMKVT+FW+G + I   S+  I   V+++L+
Sbjct: 438  AMVRKLSACETMGSATVICTDKTGTLTLNEMKVTKFWLGQESIHEDSTKMISPDVLDLLY 497

Query: 498  QAIGLNTAGSVQRQPTSSSLLPEIFGSPTEKAILSWAVFDLDLNLDELKKHHEIIQVETF 557
            Q  GLNT GSV    + S+  PE  GSPTEKA+LSW V +L ++++ +K+ HE+++VETF
Sbjct: 498  QGTGLNTTGSVCVSDSGST--PEFSGSPTEKALLSWTVLNLGMDMESVKQKHEVLRVETF 557

Query: 558  NSEKKRSGVLTRQYGERLIHTHWKGAAEMILAMCSYCYERQGTVRAIDDEGRRRLVAIIT 617
            +S KKRSGVL R+  +  +H HWKGAAEM+LAMCS+ Y   G+V  +D   + R+ AII 
Sbjct: 558  SSAKKRSGVLVRRKSDNTVHVHWKGAAEMVLAMCSHYYTSTGSVDLMDSTAKSRIQAIIQ 617

Query: 618  TMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPCRPGVRAAVESCRAA 677
             MA  SLRCIAFAHK    D  LE      E GLTL+GIVGLKDPCRPGV  AVE+C+ A
Sbjct: 618  GMAASSLRCIAFAHKIASNDSVLE------EDGLTLMGIVGLKDPCRPGVSKAVETCKLA 677

Query: 678  GVNIKMVTGDNLHTATAIAIECGILDLNDDLSSEVVVEGKEFRNYTPEERLERVGRIRVM 737
            GV IKM+TGDN+ TA AIA ECGILD ND    + VVEG +FRNYT EER+++V +IRVM
Sbjct: 678  GVTIKMITGDNVFTAKAIAFECGILDHNDKDEEDAVVEGVQFRNYTDEERMQKVDKIRVM 737

Query: 738  ARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGIQGTEVAKESSDIVI 797
            ARSSP DKLLMV+CL+ +GHVVAVTGDGTNDAPAL EADIGLSMGIQGTEVAKESSDIVI
Sbjct: 738  ARSSPSDKLLMVKCLRLKGHVVAVTGDGTNDAPALKEADIGLSMGIQGTEVAKESSDIVI 797

Query: 798  LDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVSSGKVPLTAVQLLWV 857
            LDDNF SV TVLKWGRCVYNNIQKFIQFQLTVNVAALV+NFIAA+S+G+VPLTAVQLLWV
Sbjct: 798  LDDNFASVATVLKWGRCVYNNIQKFIQFQLTVNVAALVINFIAAISAGEVPLTAVQLLWV 857

Query: 858  NLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQAVYQVTVLLVLEFKG 917
            NLIMDT+GALALATERPTN+L+ + PVGR+E L+T VMWRNL+ Q++YQ+ VLL+L+FKG
Sbjct: 858  NLIMDTLGALALATERPTNELLKRKPVGRTEALITNVMWRNLLVQSLYQIAVLLILQFKG 917

Query: 918  RSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSKLFLAIIAITIAFQV 977
             SIF+V + VK TLIFNTFVLCQ+FNEFNAR++EKKN+F+GLHR++LF+ IIAITI  QV
Sbjct: 918  MSIFSVRKEVKDTLIFNTFVLCQVFNEFNAREMEKKNVFKGLHRNRLFIGIIAITIVLQV 977

Query: 978  IMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAMEF 1017
            IMVE L +FA+T RLN  QWG+CI +A+LSWPIG+ +K IPVS   F
Sbjct: 978  IMVEFLKKFADTVRLNGWQWGTCIALASLSWPIGFFTKFIPVSETPF 1011

BLAST of MC08g2325.1 vs. ExPASy Swiss-Prot
Match: Q9LIK7 (Putative calcium-transporting ATPase 13, plasma membrane-type OS=Arabidopsis thaliana OX=3702 GN=ACA13 PE=3 SV=1)

HSP 1 Score: 1218.8 bits (3152), Expect = 0.0e+00
Identity = 636/1021 (62.29%), Postives = 790/1021 (77.38%), Query Frame = 0

Query: 4    RKNDEMVIVNHEERSSMAKNHKRRWRMAFAAICFTRVLISLSKKVGGRRKSGGLLRSLSY 63
            +K+   V V  E   +++K++K +W++A   +  +R L++ +K     RK G   RSLSY
Sbjct: 11   KKDKVGVEVLLELPKTLSKSNK-KWQLALIKLYCSRTLLNCAKH--AIRKPGLFPRSLSY 70

Query: 64   VAVDVPLNDGDSTDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGLAALLETS 123
             A+D+  + GD               +D E L+++V+ K+ + L+  GG  GL + L+++
Sbjct: 71   TAIDLDHHHGDD-----------HFKIDTETLNDLVKNKNQEKLESLGGPNGLVSALKSN 130

Query: 124  ANGGISATDDDLIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAALSLGFGI 183
               GI+   D++  RR+ FG+NTY +   +    FV+EAFKD TI+ILL CA LSLGFGI
Sbjct: 131  TRLGINEEGDEIQRRRSTFGSNTYTRQPSKGLFHFVVEAFKDLTILILLGCATLSLGFGI 190

Query: 184  KQHGWDDGWYDGGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRAGRRKPV 243
            K+HG  +GWYDGGSI +A+FLVV VSAVSNF Q+RQF+KLS    +IKIDVVR GRR+ +
Sbjct: 191  KEHGLKEGWYDGGSIFVAVFLVVAVSAVSNFRQNRQFDKLSKVSSNIKIDVVRNGRRQEI 250

Query: 244  SIFEVVVGDVVRLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPFLLSGTK 303
            SIF++VVGD+V L IGDQVPADGVF+EGH L VDESSM GES+HVEV+L  N FL SGTK
Sbjct: 251  SIFDIVVGDIVCLNIGDQVPADGVFVEGHLLHVDESSMTGESDHVEVSLTGNTFLFSGTK 310

Query: 304  VSDGFGVMMVTSVGMNTAWGEMMSSIREEVNELTPLQACLNKMTAVVGKLGLTVAVLVLL 363
            ++DGFG M VTSVGMNTAWG+MMS I  + NE TPLQ+ L+K+T+ +GK+GL VA LVLL
Sbjct: 311  IADGFGKMAVTSVGMNTAWGQMMSHISRDTNEQTPLQSRLDKLTSSIGKVGLLVAFLVLL 370

Query: 364  VLLLRYFTGSTK------EFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLT 423
            VLL+RYFTG+TK      E+NG  T  + ++NAV+ MVAAAVTI+VVAIPEGLPLAVTLT
Sbjct: 371  VLLIRYFTGTTKDESGNREYNGKTTKSDEIVNAVVKMVAAAVTIIVVAIPEGLPLAVTLT 430

Query: 424  LAYSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDRYSS 483
            LAYSMK+MM DNAMVRKLSACETMGSAT ICTDKTGTLTLN+MKVT+FW G   ++   +
Sbjct: 431  LAYSMKRMMKDNAMVRKLSACETMGSATVICTDKTGTLTLNQMKVTDFWFG---LESGKA 490

Query: 484  STIEAKVVEVLHQAIGLNTAGSVQRQPTSSSLLPEIFGSPTEKAILSWAVFDLDLNLDEL 543
            S++  +VVE+ HQ + +NT GSV +    +    E  GSPTEKAILSWAV +L++ ++++
Sbjct: 491  SSVSQRVVELFHQGVAMNTTGSVFKAKAGTEY--EFSGSPTEKAILSWAVEELEMGMEKV 550

Query: 544  KKHHEIIQVETFNSEKKRSGVLTRQYGERLIH--THWKGAAEMILAMCSYCYERQGTVRA 603
             + H+++ VE FNSEKKRSGVL ++ G    +   HWKGAAE ILAMCS   +  G VR 
Sbjct: 551  IEEHDVVHVEGFNSEKKRSGVLMKKKGVNTENNVVHWKGAAEKILAMCSTFCDGSGVVRE 610

Query: 604  IDDEGRRRLVAIITTMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPC 663
            + ++ + +   II +MA KSLRCIAFA+  + ED       KL E  L+LLGI+G+KDPC
Sbjct: 611  MKEDDKIQFEKIIQSMAAKSLRCIAFAYSEDNEDNK-----KLKEEKLSLLGIIGIKDPC 670

Query: 664  RPGVRAAVESCRAAGVNIKMVTGDNLHTATAIAIECGILDLNDDLSSEVVVEGKEFRNYT 723
            RPGV+ AVE C+ AGVNIKM+TGDN+ TA AIA+ECGIL   D+++SE V+EG++FRNYT
Sbjct: 671  RPGVKKAVEDCQFAGVNIKMITGDNIFTARAIAVECGILTPEDEMNSEAVLEGEKFRNYT 730

Query: 724  PEERLERVGRIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI 783
             EERLE+V RI+VMARSSPFDKLLMV+CLK  GHVVAVTGDGTNDAPAL EADIGLSMGI
Sbjct: 731  QEERLEKVERIKVMARSSPFDKLLMVKCLKELGHVVAVTGDGTNDAPALKEADIGLSMGI 790

Query: 784  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVS 843
            QGTEVAKESSDIVILDDNF SV TVLKWGRCVYNNIQKFIQFQLTVNVAALV+NF+AAVS
Sbjct: 791  QGTEVAKESSDIVILDDNFASVATVLKWGRCVYNNIQKFIQFQLTVNVAALVINFVAAVS 850

Query: 844  SGKVPLTAVQLLWVNLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQA 903
            +G VPLTAVQLLWVNLIMDT+GALALATE+PTNDLM K P+GR  PL+T +MWRNL+AQA
Sbjct: 851  AGDVPLTAVQLLWVNLIMDTLGALALATEKPTNDLMKKKPIGRVAPLITNIMWRNLLAQA 910

Query: 904  VYQVTVLLVLEFKGRSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSK 963
             YQ++VLLVL+F+GRSIFNV E+VK+TLIFNTFVLCQ+FNEFNAR LEKKN+F+GLH+++
Sbjct: 911  FYQISVLLVLQFRGRSIFNVTEKVKNTLIFNTFVLCQVFNEFNARSLEKKNVFKGLHKNR 970

Query: 964  LFLAIIAITIAFQVIMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAME 1017
            LF+ II +T+  QV+MVE L RFA+TERLN  QWG CI IAA SWPIGW+ K +PV    
Sbjct: 971  LFIGIIVVTVVLQVVMVEFLKRFADTERLNLGQWGVCIAIAAASWPIGWLVKSVPVPERH 1007

BLAST of MC08g2325.1 vs. ExPASy Swiss-Prot
Match: Q7XEK4 (Calcium-transporting ATPase 7, plasma membrane-type OS=Oryza sativa subsp. japonica OX=39947 GN=ACA7 PE=2 SV=1)

HSP 1 Score: 1074.7 bits (2778), Expect = 1.0e-312
Identity = 569/1003 (56.73%), Postives = 727/1003 (72.48%), Query Frame = 0

Query: 25   KRRWRMAFAAICFTRVLISLSKKVGGRRKSGGLLRSLSYVAVDV-PLNDGDSTDCGELSC 84
            +++WR A   I     L+ L         +G + R+ SYV + V    + D +  G+   
Sbjct: 30   EKQWRKATNVIRGCHRLLRLGVLSAA---AGIMRRNPSYVEIKVHDEGELDVSSGGDGEA 89

Query: 85   MAAFS-DVDREVLSEMVREKDSDCLDRFGGVEGLAALLETSANGGISATDDDLIARRNAF 144
              AF+   D E    +V+ K  DC    GG  G+AA+L + A  GI   D D+  R+ AF
Sbjct: 90   PVAFTVAADDESFKGLVKNKREDCFRLLGGGAGVAAVLASGAERGIRGDDADVARRKKAF 149

Query: 145  GANTYPKLHGRSFLSFVIEAFKDTTIIILLICAALSLGFGIKQHGWDDGWYDGGSIVIAI 204
            G+NTYPK   + F   V +A  D  +I+LL+CAA+SL FGIK+HG  DGWYDG SI +A+
Sbjct: 150  GSNTYPKPKPKGFFRHVWDALADVFLIVLLVCAAVSLAFGIKEHGIKDGWYDGVSIFLAV 209

Query: 205  FLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRAGRRKPVSIFEVVVGDVVRLKIGDQV 264
            FLV  VSAVSN +Q ++F+KL+ E ++I + VVRA RR+ VSIF+VVVGDVV LKIGD V
Sbjct: 210  FLVAAVSAVSNHSQGKRFDKLARESENIMVSVVRAARRQEVSIFDVVVGDVVVLKIGDVV 269

Query: 265  PADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPFLLSGTKVSDGFGVMMVTSVGMNTAW 324
            PADGVFL+GH+L+VDESSM GE   VEV+   +PFL SG KV DG+G M+VT+VG +TAW
Sbjct: 270  PADGVFLDGHALQVDESSMTGEPHPVEVDAVKSPFLASGVKVVDGYGKMVVTAVGTDTAW 329

Query: 325  GEMMSSIREEVNELTPLQACLNKMTAVVGKLGLTVAVLVLLVLLLRYFTGSTKE------ 384
            GEMM +I  E  + TPLQ  L  +T+ +GK+G+ VAVLV  VL  R+FTGST++      
Sbjct: 330  GEMMRTITRENTDPTPLQERLEGLTSSIGKVGIAVAVLVFAVLTARHFTGSTRDEQGNAL 389

Query: 385  FNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLAYSMKKMMADNAMVRKLS 444
            F+    +FN+V + ++G+   AVTI+VVAIPEGLPLAVTLTLA+SMK+M+ +NA+VR+LS
Sbjct: 390  FDKRNVTFNAVFSGLVGIFQQAVTIIVVAIPEGLPLAVTLTLAFSMKRMVRENALVRRLS 449

Query: 445  ACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDRYSSSTIEAKVVEVLHQAIGLNT 504
            ACETMGS T ICTDKTGTLTLN+MKVTEFW+GAD     S++ +   VV +L Q  GLNT
Sbjct: 450  ACETMGSVTAICTDKTGTLTLNQMKVTEFWVGADR--PRSAAAVNGGVVRLLCQGAGLNT 509

Query: 505  AGSVQRQPTSSSLLPEIFGSPTEKAILSWAVFDLDLNLDELKKHHEIIQVETFNSEKKRS 564
             GSV +    S   PEI GSPTEKA+LSWAV +L ++ D LK+  ++++VE FNS+KKRS
Sbjct: 510  TGSVYKPDNVSP--PEITGSPTEKALLSWAVEELPMDADALKRKCKVVRVEAFNSDKKRS 569

Query: 565  GVLTRQYGERLIHTHWKGAAEMILAMCSYCYERQGTVRAIDDEGRRRLVAIITTMAEKSL 624
            GV+ R      +  HWKGAAEM+LA C+      G  R +  E RR+L  +I  MA  SL
Sbjct: 570  GVMLRDAATGAVTAHWKGAAEMVLARCTVYVGADGAARELGVEQRRKLEQVINDMAAASL 629

Query: 625  RCIAFAHK---TNGEDQNLETPAKLDESGLTLLGIVGLKDPCRPGVRAAVESCRAAGVNI 684
            RCIAFA+K     G+  N    AK+D+ GLTLLG VGLKDPCRP V++A+E+C  AG+ +
Sbjct: 630  RCIAFAYKQVVDGGDSDN----AKIDDEGLTLLGFVGLKDPCRPEVKSAIEACTKAGIAV 689

Query: 685  KMVTGDNLHTATAIAIECGILDLNDDLSSEVVVEGKEFRNYTPEERLERVGRIRVMARSS 744
            KMVTGDN+ TA AIA ECGI+  NDD ++ VV+EG EFR  + +E+L  V  IRVMARS 
Sbjct: 690  KMVTGDNVLTARAIAKECGIISGNDDDAAGVVIEGHEFRAMSEQEQLAIVDNIRVMARSL 749

Query: 745  PFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGIQGTEVAKESSDIVILDDN 804
            P DKL++VQ LK +GHVVAVTGDGTNDAPAL EAD+GLSMG+QGTEVAKESSDIVIL+DN
Sbjct: 750  PLDKLVLVQRLKQKGHVVAVTGDGTNDAPALKEADVGLSMGVQGTEVAKESSDIVILNDN 809

Query: 805  FTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVSSGKVPLTAVQLLWVNLIM 864
            F +VVT  +WGRCVYNNIQKFIQFQLTVNVAALV+NF++AV++G++PLT VQLLWVNLIM
Sbjct: 810  FDTVVTATRWGRCVYNNIQKFIQFQLTVNVAALVINFVSAVTTGRMPLTTVQLLWVNLIM 869

Query: 865  DTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQAVYQVTVLLVLEFKGRSIF 924
            DT+GALALAT+ PT  LM + P+GR+ PL++  MWRNL AQA YQV VLL L+++G    
Sbjct: 870  DTMGALALATDTPTAGLMRRPPIGRAAPLISNAMWRNLAAQAAYQVAVLLALQYRGFGGA 929

Query: 925  NVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSKLFLAIIAITIAFQVIMVE 984
               ER   T+IFN FVLCQ+FNEFNAR++E++N+F G+HR+++FL I+A+T+A QV+MVE
Sbjct: 930  GAGERANGTMIFNAFVLCQVFNEFNAREIERRNVFAGVHRNRMFLGIVAVTVALQVVMVE 989

Query: 985  LLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAMEF 1017
            LL +FA TERL W QWG+C+GIAA+SWPIGW  K IPV    F
Sbjct: 990  LLTKFAGTERLGWGQWGACVGIAAVSWPIGWAVKCIPVPERPF 1021

BLAST of MC08g2325.1 vs. ExPASy Swiss-Prot
Match: Q9SZR1 (Calcium-transporting ATPase 10, plasma membrane-type OS=Arabidopsis thaliana OX=3702 GN=ACA10 PE=1 SV=2)

HSP 1 Score: 977.2 bits (2525), Expect = 1.4e-283
Identity = 525/939 (55.91%), Postives = 679/939 (72.31%), Query Frame = 0

Query: 90   VDREVLSEMVREKDSDCLDRFGGVEGLAALLETSANGGISATDDDLIARRNAFGANTYPK 149
            + +E +  + R+++   L   GGV GL+ LL+T+   GI   DDD++ R++AFG+NTYP+
Sbjct: 116  IGQEQIVSISRDQNIGALQELGGVRGLSDLLKTNLEKGIHGDDDDILKRKSAFGSNTYPQ 175

Query: 150  LHGRSFLSFVIEAFKDTTIIILLICAALSLGFGIKQHGWDDGWYDGGSIVIAIFLVVVVS 209
              GRSF  FV EA +D T+IIL++ A  SL  GIK  G + GWYDG SI  A+ LV+VV+
Sbjct: 176  KKGRSFWRFVWEASQDLTLIILIVAAVASLALGIKTEGIEKGWYDGISIAFAVLLVIVVT 235

Query: 210  AVSNFNQSRQFEKLSDERDDIKIDVVRAGRRKPVSIFEVVVGDVVRLKIGDQVPADGVFL 269
            A S++ QS QF+ L++E+ +I+++V R GRR  +SI+++VVGDV+ L IGDQVPADGV +
Sbjct: 236  ATSDYRQSLQFQNLNEEKRNIRLEVTRDGRRVEISIYDIVVGDVIPLNIGDQVPADGVLV 295

Query: 270  EGHSLKVDESSMNGESEHVEVNLGTNPFLLSGTKVSDGFGVMMVTSVGMNTAWGEMMSSI 329
             GHSL VDESSM GES+ V+ N   +PFL+SG KV+DG G M+VT VG+NT WG +M+S+
Sbjct: 296  AGHSLAVDESSMTGESKIVQKNSTKHPFLMSGCKVADGNGTMLVTGVGVNTEWGLLMASV 355

Query: 330  REEVNELTPLQACLNKMTAVVGKLGLTVAVLVLLVLLLRYFTGSTK------EFNGSKTS 389
             E+    TPLQ  LN +   +G +GLTVA +VL VL++RYFTG TK      +F G KT 
Sbjct: 356  SEDNGGETPLQVRLNGVATFIGIVGLTVAGVVLFVLVVRYFTGHTKNEQGGPQFIGGKTK 415

Query: 390  FNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLAYSMKKMMADNAMVRKLSACETMGS 449
            F  V++ ++ +   AVTIVVVA+PEGLPLAVTLTLAYSM+KMMAD A+VR+LSACETMGS
Sbjct: 416  FEHVLDDLVEIFTVAVTIVVVAVPEGLPLAVTLTLAYSMRKMMADKALVRRLSACETMGS 475

Query: 450  ATTICTDKTGTLTLNEMKVTEFWIGADEIDR-YSSSTIEAKVVEVLHQAIGLNTAGSVQR 509
            ATTIC+DKTGTLTLNEM V E + G  ++D   SSS + +    +L + I  NT GSV R
Sbjct: 476  ATTICSDKTGTLTLNEMTVVECYAGLQKMDSPDSSSKLPSAFTSILVEGIAHNTTGSVFR 535

Query: 510  QPTSSSLLPEIFGSPTEKAILSWAVFDLDLNLDELKKHHEIIQVETFNSEKKRSGVLTRQ 569
               S S   ++ GSPTE+AIL+WA+  L ++ D LK     +Q   FNSEKKR GV  + 
Sbjct: 536  ---SESGEIQVSGSPTERAILNWAI-KLGMDFDALKSESSAVQFFPFNSEKKRGGVAVKS 595

Query: 570  YGERLIHTHWKGAAEMILAMCSYCYERQGTVRAIDDEGRRRLVAIITTMAEKSLRCIAFA 629
              +  +H HWKGAAE++L  C++  +   +   + ++    L   I  MA +SLRC+A A
Sbjct: 596  -PDSSVHIHWKGAAEIVLGSCTHYMDESESFVDMSEDKMGGLKDAIDDMAARSLRCVAIA 655

Query: 630  HKT-----NGEDQNLETPAKLDESGLTLLGIVGLKDPCRPGVRAAVESCRAAGVNIKMVT 689
             +T        D+   +  +L E  L LL IVG+KDPCRPGV+ +V  C+ AGV ++MVT
Sbjct: 656  FRTFEADKIPTDEEQLSRWELPEDDLILLAIVGIKDPCRPGVKNSVLLCQQAGVKVRMVT 715

Query: 690  GDNLHTATAIAIECGILDLNDDLSSEVVVEGKEFRNYTPEERLERVGRIRVMARSSPFDK 749
            GDN+ TA AIA+ECGIL  + D S   ++EGK FR+Y+ EER      I VM RSSP DK
Sbjct: 716  GDNIQTAKAIALECGILASDSDASEPNLIEGKVFRSYSEEERDRICEEISVMGRSSPNDK 775

Query: 750  LLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGIQGTEVAKESSDIVILDDNFTSV 809
            LL+VQ LK +GHVVAVTGDGTNDAPALHEADIGL+MGIQGTEVAKE SDI+ILDDNF SV
Sbjct: 776  LLLVQSLKRRGHVVAVTGDGTNDAPALHEADIGLAMGIQGTEVAKEKSDIIILDDNFESV 835

Query: 810  VTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVSSGKVPLTAVQLLWVNLIMDTVG 869
            V V++WGR VY NIQKFIQFQLTVNVAALV+N +AA+S+G+VPLTAVQLLWVNLIMDT+G
Sbjct: 836  VKVVRWGRSVYANIQKFIQFQLTVNVAALVINVVAAISAGEVPLTAVQLLWVNLIMDTLG 895

Query: 870  ALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQAVYQVTVLLVLEFKGRSIFNVN- 929
            ALALATE PT+ LM + PVGR EPL+T +MWRNL  QA+YQVTVLL+L F+G SI ++  
Sbjct: 896  ALALATEPPTDHLMDRAPVGRREPLITNIMWRNLFIQAMYQVTVLLILNFRGISILHLKS 955

Query: 930  ----ERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSKLFLAIIAITIAFQVIMV 989
                ERVK+T+IFN FV+CQ+FNEFNARK ++ NIF G+ R+ LF+ II+ITI  QV++V
Sbjct: 956  KPNAERVKNTVIFNAFVICQVFNEFNARKPDEINIFRGVLRNHLFVGIISITIVLQVVIV 1015

Query: 990  ELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPV 1012
            E LG FA+T +L+W  W  CIGI ++SWP+  + KLIPV
Sbjct: 1016 EFLGTFASTTKLDWEMWLVCIGIGSISWPLAVIGKLIPV 1049

BLAST of MC08g2325.1 vs. ExPASy Swiss-Prot
Match: Q9LF79 (Calcium-transporting ATPase 8, plasma membrane-type OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=1 SV=1)

HSP 1 Score: 976.5 bits (2523), Expect = 2.4e-283
Identity = 522/939 (55.59%), Postives = 669/939 (71.25%), Query Frame = 0

Query: 93   EVLSEMVREKDSDCLDRFGGVEGLAALLETSANGGISATDDDLIARRNAFGANTYPKLHG 152
            E L  M ++ +S  L+++GG +GLA LL+T+   GIS  DDDL+ R+  +G+NTYP+  G
Sbjct: 119  EQLVIMSKDHNSGALEQYGGTQGLANLLKTNPEKGISGDDDDLLKRKTIYGSNTYPRKKG 178

Query: 153  RSFLSFVIEAFKDTTIIILLICAALSLGFGIKQHGWDDGWYDGGSIVIAIFLVVVVSAVS 212
            + FL F+ +A  D T+IIL++ A  SL  GIK  G  +GWYDGGSI  A+ LV+VV+AVS
Sbjct: 179  KGFLRFLWDACHDLTLIILMVAAVASLALGIKTEGIKEGWYDGGSIAFAVILVIVVTAVS 238

Query: 213  NFNQSRQFEKLSDERDDIKIDVVRAGRRKPVSIFEVVVGDVVRLKIGDQVPADGVFLEGH 272
            ++ QS QF+ L+DE+ +I ++V+R GRR  +SI+++VVGDV+ L IG+QVPADGV + GH
Sbjct: 239  DYKQSLQFQNLNDEKRNIHLEVLRGGRRVEISIYDIVVGDVIPLNIGNQVPADGVLISGH 298

Query: 273  SLKVDESSMNGESEHVEVNLGTNPFLLSGTKVSDGFGVMMVTSVGMNTAWGEMMSSIREE 332
            SL +DESSM GES+ V  +   +PFL+SG KV+DG G M+VT VG+NT WG +M+SI E+
Sbjct: 299  SLALDESSMTGESKIVNKDANKDPFLMSGCKVADGNGSMLVTGVGVNTEWGLLMASISED 358

Query: 333  VNELTPLQACLNKMTAVVGKLGLTVAVLVLLVLLLRYFTGSTKEFNG------SKTSFNS 392
              E TPLQ  LN +   +G +GL VA  VL++LL RYFTG TK+ NG       KT    
Sbjct: 359  NGEETPLQVRLNGVATFIGSIGLAVAAAVLVILLTRYFTGHTKDNNGGPQFVKGKTKVGH 418

Query: 393  VMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLAYSMKKMMADNAMVRKLSACETMGSATT 452
            V++ V+ ++  AVTIVVVA+PEGLPLAVTLTLAYSM+KMMAD A+VR+LSACETMGSATT
Sbjct: 419  VIDDVVKVLTVAVTIVVVAVPEGLPLAVTLTLAYSMRKMMADKALVRRLSACETMGSATT 478

Query: 453  ICTDKTGTLTLNEMKVTEFWIGADEIDRYSSSTIEAKVVEVLHQAIGLNTAGSVQRQPTS 512
            IC+DKTGTLTLN+M V E + G  + D   +  + A +  ++ + I  NT GS+      
Sbjct: 479  ICSDKTGTLTLNQMTVVESYAGGKKTD---TEQLPATITSLVVEGISQNTTGSIFVPEGG 538

Query: 513  SSLLPEIFGSPTEKAILSWAVFDLDLNLDELKKHHEIIQVETFNSEKKRSGVLTRQYGER 572
              L  E  GSPTEKAIL W V  L +N +  +    I+    FNSEKKR GV  +   + 
Sbjct: 539  GDL--EYSGSPTEKAILGWGV-KLGMNFETARSQSSILHAFPFNSEKKRGGVAVKT-ADG 598

Query: 573  LIHTHWKGAAEMILAMCSYCYERQGTVRAIDDEGRRRLVAIITTMAEKSLRCIAFAHKTN 632
             +H HWKGA+E++LA C    +  G V  + D+        I  MA ++LRC+A A +T 
Sbjct: 599  EVHVHWKGASEIVLASCRSYIDEDGNVAPMTDDKASFFKNGINDMAGRTLRCVALAFRTY 658

Query: 633  GEDQNLETPAKLD-----ESGLTLLGIVGLKDPCRPGVRAAVESCRAAGVNIKMVTGDNL 692
             E + + T  +L      E  L LL IVG+KDPCRPGV+ +V  C+ AGV ++MVTGDN+
Sbjct: 659  -EAEKVPTGEELSKWVLPEDDLILLAIVGIKDPCRPGVKDSVVLCQNAGVKVRMVTGDNV 718

Query: 693  HTATAIAIECGILDLNDDLSSEVVVEGKEFRNYTPEERLERVGRIRVMARSSPFDKLLMV 752
             TA AIA+ECGIL  + DLS   ++EGK FR  T  ER +   +I VM RSSP DKLL+V
Sbjct: 719  QTARAIALECGILSSDADLSEPTLIEGKSFREMTDAERDKISDKISVMGRSSPNDKLLLV 778

Query: 753  QCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGIQGTEVAKESSDIVILDDNFTSVVTVL 812
            Q L+ QGHVVAVTGDGTNDAPALHEADIGL+MGI GTEVAKESSDI+ILDDNF SVV V+
Sbjct: 779  QSLRRQGHVVAVTGDGTNDAPALHEADIGLAMGIAGTEVAKESSDIIILDDNFASVVKVV 838

Query: 813  KWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVSSGKVPLTAVQLLWVNLIMDTVGALAL 872
            +WGR VY NIQKFIQFQLTVNVAALV+N +AA+SSG VPLTAVQLLWVNLIMDT+GALAL
Sbjct: 839  RWGRSVYANIQKFIQFQLTVNVAALVINVVAAISSGDVPLTAVQLLWVNLIMDTLGALAL 898

Query: 873  ATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQAVYQVTVLLVLEFKGRSIFNVNE---- 932
            ATE PT+ LM + PVGR EPL+T +MWRNL+ QA+YQV+VLL L F+G SI  +      
Sbjct: 899  ATEPPTDHLMGRPPVGRKEPLITNIMWRNLLIQAIYQVSVLLTLNFRGISILGLEHEVHE 958

Query: 933  ---RVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSKLFLAIIAITIAFQVIMVEL 992
               RVK+T+IFN FVLCQ FNEFNARK ++KNIF+G+ +++LF+ II IT+  QVI+VE 
Sbjct: 959  HATRVKNTIIFNAFVLCQAFNEFNARKPDEKNIFKGVIKNRLFMGIIVITLVLQVIIVEF 1018

Query: 993  LGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSA 1014
            LG+FA+T +LNW QW  C+GI  +SWP+  V K IPV A
Sbjct: 1019 LGKFASTTKLNWKQWLICVGIGVISWPLALVGKFIPVPA 1049

BLAST of MC08g2325.1 vs. NCBI nr
Match: XP_022142981.1 (putative calcium-transporting ATPase 13, plasma membrane-type [Momordica charantia])

HSP 1 Score: 1962 bits (5082), Expect = 0.0
Identity = 1018/1019 (99.90%), Postives = 1018/1019 (99.90%), Query Frame = 0

Query: 1    LRYRKNDEMVIVNHEERSSMAKNHKRRWRMAFAAICFTRVLISLSKKVGGRRKSGGLLRS 60
            LRYRKNDEMVIVNHEERSSMAKNHKRRWRMAFAAICFTRVLISLSKKVGGRRKSGGLLRS
Sbjct: 3    LRYRKNDEMVIVNHEERSSMAKNHKRRWRMAFAAICFTRVLISLSKKVGGRRKSGGLLRS 62

Query: 61   LSYVAVDVPLNDGDSTDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGLAALL 120
            LSYVAVDVPLNDGDSTDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGLAALL
Sbjct: 63   LSYVAVDVPLNDGDSTDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGLAALL 122

Query: 121  ETSANGGISATDDDLIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAALSLG 180
            ETSANGGISATDDDLIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAALSLG
Sbjct: 123  ETSANGGISATDDDLIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAALSLG 182

Query: 181  FGIKQHGWDDGWYDGGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRAGRR 240
            FGIKQHGWDDGWYDGGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRAGRR
Sbjct: 183  FGIKQHGWDDGWYDGGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRAGRR 242

Query: 241  KPVSIFEVVVGDVVRLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPFLLS 300
            KPVSIFEVVVGDVVRLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPFLLS
Sbjct: 243  KPVSIFEVVVGDVVRLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPFLLS 302

Query: 301  GTKVSDGFGVMMVTSVGMNTAWGEMMSSIREEVNELTPLQACLNKMTAVVGKLGLTVAVL 360
            GTKVSDGFGVMMVTSVGMNTAWGEMMSSIREEVNELTPLQA LNKMTAVVGKLGLTVAVL
Sbjct: 303  GTKVSDGFGVMMVTSVGMNTAWGEMMSSIREEVNELTPLQARLNKMTAVVGKLGLTVAVL 362

Query: 361  VLLVLLLRYFTGSTKEFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLAY 420
            VLLVLLLRYFTGSTKEFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLAY
Sbjct: 363  VLLVLLLRYFTGSTKEFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLAY 422

Query: 421  SMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDRYSSSTI 480
            SMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDRYSSSTI
Sbjct: 423  SMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDRYSSSTI 482

Query: 481  EAKVVEVLHQAIGLNTAGSVQRQPTSSSLLPEIFGSPTEKAILSWAVFDLDLNLDELKKH 540
            EAKVVEVLHQAIGLNTAGSVQRQPTSSSLLPEIFGSPTEKAILSWAVFDLDLNLDELKKH
Sbjct: 483  EAKVVEVLHQAIGLNTAGSVQRQPTSSSLLPEIFGSPTEKAILSWAVFDLDLNLDELKKH 542

Query: 541  HEIIQVETFNSEKKRSGVLTRQYGERLIHTHWKGAAEMILAMCSYCYERQGTVRAIDDEG 600
            HEIIQVETFNSEKKRSGVLTRQYGERLIHTHWKGAAEMILAMCSYCYERQGTVRAIDDEG
Sbjct: 543  HEIIQVETFNSEKKRSGVLTRQYGERLIHTHWKGAAEMILAMCSYCYERQGTVRAIDDEG 602

Query: 601  RRRLVAIITTMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPCRPGVR 660
            RRRLVAIITTMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPCRPGVR
Sbjct: 603  RRRLVAIITTMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPCRPGVR 662

Query: 661  AAVESCRAAGVNIKMVTGDNLHTATAIAIECGILDLNDDLSSEVVVEGKEFRNYTPEERL 720
            AAVESCRAAGVNIKMVTGDNLHTATAIAIECGILDLNDDLSSEVVVEGKEFRNYTPEERL
Sbjct: 663  AAVESCRAAGVNIKMVTGDNLHTATAIAIECGILDLNDDLSSEVVVEGKEFRNYTPEERL 722

Query: 721  ERVGRIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGIQGTEV 780
            ERVGRIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGIQGTEV
Sbjct: 723  ERVGRIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGIQGTEV 782

Query: 781  AKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVSSGKVP 840
            AKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVSSGKVP
Sbjct: 783  AKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVSSGKVP 842

Query: 841  LTAVQLLWVNLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQAVYQVT 900
            LTAVQLLWVNLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQAVYQVT
Sbjct: 843  LTAVQLLWVNLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQAVYQVT 902

Query: 901  VLLVLEFKGRSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSKLFLAI 960
            VLLVLEFKGRSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSKLFLAI
Sbjct: 903  VLLVLEFKGRSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSKLFLAI 962

Query: 961  IAITIAFQVIMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAMEFPRR 1019
            IAITIAFQVIMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAMEFPRR
Sbjct: 963  IAITIAFQVIMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAMEFPRR 1021

BLAST of MC08g2325.1 vs. NCBI nr
Match: XP_022965553.1 (putative calcium-transporting ATPase 13, plasma membrane-type [Cucurbita maxima] >XP_022965554.1 putative calcium-transporting ATPase 13, plasma membrane-type [Cucurbita maxima])

HSP 1 Score: 1622 bits (4201), Expect = 0.0
Identity = 848/1023 (82.89%), Postives = 923/1023 (90.22%), Query Frame = 0

Query: 1    LRYRKNDEMV-IVNHEERSSMAKNHKRRWRMAFAAICFTRVLISLSKKVGGRRKSGGLLR 60
            LR RK+DE+V ++N EER  MAK HKRRW+MAFAAI FTR LISL++KV  R KSG LL 
Sbjct: 3    LRCRKSDEVVEVLNREERLVMAKTHKRRWKMAFAAIYFTRALISLTRKVTDR-KSGALLP 62

Query: 61   SLSYVAVDVPLNDGDSTDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGLAAL 120
            SLS+V +DV +ND DSTD GE  CMA F  VD++VLSEMVREKD +CL+RFGGV+GLA L
Sbjct: 63   SLSFVTLDVQVND-DSTDSGEQPCMAGFFGVDQKVLSEMVREKDCECLNRFGGVDGLANL 122

Query: 121  LETSANGGISATDDDLIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAALSL 180
            L+T  + GI AT DDL  R NAFG NTY K HG+SF S+VIEAF DTTIIILLICA LSL
Sbjct: 123  LKTDPHDGICATQDDLTYRTNAFGTNTYSKRHGKSFFSYVIEAFNDTTIIILLICAVLSL 182

Query: 181  GFGIKQHGWDDGWYDGGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRAGR 240
            GFGIKQHGWDDGWYDGGSIVIAIFLVVVVSA SNF QSRQFEKLS+ERDDIKI+V RAGR
Sbjct: 183  GFGIKQHGWDDGWYDGGSIVIAIFLVVVVSAASNFKQSRQFEKLSNERDDIKIEVTRAGR 242

Query: 241  RKPVSIFEVVVGDVVRLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPFLL 300
            RK VSIFE++VGD+V LKIGDQ+PADGVFL+GHSLK+DESSM GESEH EVN G NPFLL
Sbjct: 243  RKSVSIFELLVGDIVYLKIGDQIPADGVFLDGHSLKIDESSMTGESEHAEVNHGINPFLL 302

Query: 301  SGTKVSDGFGVMMVTSVGMNTAWGEMMSSIREEVNELTPLQACLNKMTAVVGKLGLTVAV 360
            SGTKV+DGFGVMMVTSVGMNTAWGEMMSSIR E+NE TPLQ  LNKMTA++GKLGLTVAV
Sbjct: 303  SGTKVNDGFGVMMVTSVGMNTAWGEMMSSIRREINEETPLQGRLNKMTAIIGKLGLTVAV 362

Query: 361  LVLLVLLLRYFTGSTKEFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA 420
            +VLLVLL+RYFTGST EFNGSKT F+ VMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA
Sbjct: 363  VVLLVLLVRYFTGSTGEFNGSKTRFDDVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA 422

Query: 421  YSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDR--YSS 480
            YSMKKMMADNAMVRKLSACET+GSATTICTDKTGTLTLN+MKVTEFWIG DEI    YS+
Sbjct: 423  YSMKKMMADNAMVRKLSACETLGSATTICTDKTGTLTLNQMKVTEFWIGEDEIMNIDYSN 482

Query: 481  STIEAKVVEVLHQAIGLNTAGSVQRQPTSSSLLP-EIFGSPTEKAILSWAVFDLDLNLDE 540
            S I +KVVE+LHQA+GLNT GS+Q   T +S LP E FGSPTEKAILSWA FDLDLNLDE
Sbjct: 483  SRISSKVVELLHQAVGLNTTGSIQ---TPASSLPLETFGSPTEKAILSWAAFDLDLNLDE 542

Query: 541  LKKHHEIIQVETFNSEKKRSGVLTRQYGERLIHTHWKGAAEMILAMCSYCYERQGTVRAI 600
            LKK HEII VETF+SEKKRSGV TR++G RL++THWKGAAE+ILAMCSY YE+QGT RAI
Sbjct: 543  LKKQHEIILVETFSSEKKRSGVSTRRHGTRLVNTHWKGAAEIILAMCSYYYEKQGTARAI 602

Query: 601  DDEGRRRLVAIITTMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPCR 660
            D E RR+L  IIT MAEKSLRCIAFAHK+N +D   + PAKLDES LTLLGIVGLKDPCR
Sbjct: 603  DGEMRRQLTEIITAMAEKSLRCIAFAHKSNEDDDIRDVPAKLDESELTLLGIVGLKDPCR 662

Query: 661  PGVRAAVESCRAAGVNIKMVTGDNLHTATAIAIECGILDLNDDLS-SEVVVEGKEFRNYT 720
             GVR AVESCRAAGV+IKMVTGDNLHTATAIAIECGIL+ +DD + SEVVVEG +FRNYT
Sbjct: 663  AGVREAVESCRAAGVSIKMVTGDNLHTATAIAIECGILNPDDDATNSEVVVEGVKFRNYT 722

Query: 721  PEERLERVGRIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI 780
            PEERLE++G IRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI
Sbjct: 723  PEERLEKIGNIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI 782

Query: 781  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVS 840
            QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVN+AALV+NFIAAVS
Sbjct: 783  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNIAALVINFIAAVS 842

Query: 841  SGKVPLTAVQLLWVNLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQA 900
            SGKVPLTAVQLLWVNLIMDT+GALALATE PTNDLM KTPVGRS+PLVTK+MWRNLI QA
Sbjct: 843  SGKVPLTAVQLLWVNLIMDTMGALALATELPTNDLMTKTPVGRSDPLVTKIMWRNLITQA 902

Query: 901  VYQVTVLLVLEFKGRSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSK 960
            VYQVTVLLVLEFKG SIFNVNE+VKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLH+SK
Sbjct: 903  VYQVTVLLVLEFKGSSIFNVNEKVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHKSK 962

Query: 961  LFLAIIAITIAFQVIMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAME 1018
            LFLAII IT+AFQV+MVE+LGRFANTERLNW QWG+C+GIAALSWPIGW+SKLIPVSA++
Sbjct: 963  LFLAIIVITLAFQVVMVEVLGRFANTERLNWGQWGACLGIAALSWPIGWLSKLIPVSALQ 1020

BLAST of MC08g2325.1 vs. NCBI nr
Match: XP_023521126.1 (putative calcium-transporting ATPase 13, plasma membrane-type [Cucurbita pepo subsp. pepo] >XP_023521127.1 putative calcium-transporting ATPase 13, plasma membrane-type [Cucurbita pepo subsp. pepo] >XP_023537101.1 putative calcium-transporting ATPase 13, plasma membrane-type [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1620 bits (4195), Expect = 0.0
Identity = 847/1022 (82.88%), Postives = 924/1022 (90.41%), Query Frame = 0

Query: 1    LRYRKNDEMV-IVNHEERSSMAKNHKRRWRMAFAAICFTRVLISLSKKVGGRRKSGGLLR 60
            LR R++DE+V ++N EER  MAK HKRRW+MAFAAI FTR L+SL++KV  R KSG LLR
Sbjct: 3    LRCRRSDEVVEVLNREERLVMAKAHKRRWKMAFAAIYFTRALMSLTRKVTDR-KSGALLR 62

Query: 61   SLSYVAVDVPLNDGDSTDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGLAAL 120
            SLS+V +DV +ND DSTD GE  CMA F  VD++VLSEMVREKD +CL+RFGGV+GLA L
Sbjct: 63   SLSFVTLDVQVND-DSTDSGEQPCMAGFFGVDQKVLSEMVREKDCECLNRFGGVDGLANL 122

Query: 121  LETSANGGISATDDDLIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAALSL 180
            L+T+ + GI AT+DDL  R NAFG NTY K HG+SF  FVIEAF DTTIIILLICA LSL
Sbjct: 123  LKTNPHDGICATEDDLNYRTNAFGTNTYSKRHGKSFFRFVIEAFNDTTIIILLICAVLSL 182

Query: 181  GFGIKQHGWDDGWYDGGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRAGR 240
            GFGIKQHGWDDGWYDGGSIVIAIFLVVVVSA SNF QSRQFEKLS+ERDDIKI+V RAGR
Sbjct: 183  GFGIKQHGWDDGWYDGGSIVIAIFLVVVVSAASNFKQSRQFEKLSNERDDIKIEVTRAGR 242

Query: 241  RKPVSIFEVVVGDVVRLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPFLL 300
            RK VSIFE++VGD+V LKIGDQ+PADGVFL+GHSLK+DESSM GESEH EVN   NPFLL
Sbjct: 243  RKSVSIFELLVGDIVYLKIGDQIPADGVFLDGHSLKIDESSMTGESEHAEVNHEINPFLL 302

Query: 301  SGTKVSDGFGVMMVTSVGMNTAWGEMMSSIREEVNELTPLQACLNKMTAVVGKLGLTVAV 360
            SGTKV+DGFGVMMVTSVGMNTAWGEMMSSIR E+NE TPLQA LNKMTA++GKLGLTVAV
Sbjct: 303  SGTKVNDGFGVMMVTSVGMNTAWGEMMSSIRREINEETPLQARLNKMTAIIGKLGLTVAV 362

Query: 361  LVLLVLLLRYFTGSTKEFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA 420
            +VLLVLL RYFTGST EFNGSKT F+ VMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA
Sbjct: 363  VVLLVLLARYFTGSTGEFNGSKTRFDDVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA 422

Query: 421  YSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDR--YSS 480
            YSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLN+MKVTEFWIG D+I    YS+
Sbjct: 423  YSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNQMKVTEFWIGEDKIMNTDYSN 482

Query: 481  STIEAKVVEVLHQAIGLNTAGSVQRQPTSSSLLP-EIFGSPTEKAILSWAVFDLDLNLDE 540
            S I +KVVE+LHQA+GLNT GS+Q   T +S LP EIFGSPTEKAILSWAVFDLDLNLDE
Sbjct: 483  SRISSKVVELLHQAVGLNTTGSIQ---TPASSLPLEIFGSPTEKAILSWAVFDLDLNLDE 542

Query: 541  LKKHHEIIQVETFNSEKKRSGVLTRQYGERLIHTHWKGAAEMILAMCSYCYERQGTVRAI 600
            LKK HE+I VETF+SEKKRSGVLTR++G RL++THWKGAAEMILAMCSY YE+QGT RAI
Sbjct: 543  LKKQHEMILVETFSSEKKRSGVLTRRHGTRLVNTHWKGAAEMILAMCSYYYEKQGTARAI 602

Query: 601  DDEGRRRLVAIITTMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPCR 660
            D E RR+L  IIT MAEKSLRCIAFAHK+N +D   + PAKLDES LTLLGIVGLKDPCR
Sbjct: 603  DGEMRRQLTEIITAMAEKSLRCIAFAHKSNEDDDIRDVPAKLDESELTLLGIVGLKDPCR 662

Query: 661  PGVRAAVESCRAAGVNIKMVTGDNLHTATAIAIECGILDLNDD-LSSEVVVEGKEFRNYT 720
            PG R AVESCRAAGV+IKMVTGDNLHTATAIAIECGIL+ +DD ++SEVVVEG +FRNYT
Sbjct: 663  PGAREAVESCRAAGVSIKMVTGDNLHTATAIAIECGILNPDDDAMNSEVVVEGVKFRNYT 722

Query: 721  PEERLERVGRIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI 780
            PEERLE++G IRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI
Sbjct: 723  PEERLEKIGTIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI 782

Query: 781  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVS 840
            QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVN+AALV+NFIAAVS
Sbjct: 783  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNIAALVINFIAAVS 842

Query: 841  SGKVPLTAVQLLWVNLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQA 900
            SGKVPLTAVQLLWVNLIMDT+GALALATE PTNDLM KTPVGRS PLVTK+MWRNLI QA
Sbjct: 843  SGKVPLTAVQLLWVNLIMDTMGALALATELPTNDLMTKTPVGRSNPLVTKIMWRNLITQA 902

Query: 901  VYQVTVLLVLEFKGRSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSK 960
            VYQVTVLLVLEFKG SIFNVNE+VKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLH+SK
Sbjct: 903  VYQVTVLLVLEFKGSSIFNVNEKVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHKSK 962

Query: 961  LFLAIIAITIAFQVIMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAME 1017
            LFLAII IT+AFQV+MVE+LGRFANT RLNW QWG+C+GIAALSWPIGW+SKLIPVSA++
Sbjct: 963  LFLAIIVITLAFQVVMVEVLGRFANTSRLNWGQWGACLGIAALSWPIGWLSKLIPVSALQ 1019

BLAST of MC08g2325.1 vs. NCBI nr
Match: XP_022937658.1 (putative calcium-transporting ATPase 13, plasma membrane-type [Cucurbita moschata])

HSP 1 Score: 1620 bits (4195), Expect = 0.0
Identity = 845/1022 (82.68%), Postives = 924/1022 (90.41%), Query Frame = 0

Query: 1    LRYRKNDEMV-IVNHEERSSMAKNHKRRWRMAFAAICFTRVLISLSKKVGGRRKSGGLLR 60
            LR RK+DE+V ++N EER  MAK HKRRW+MAFAAI FTR LISL++KV G  KSG LLR
Sbjct: 3    LRCRKSDEVVEVLNREERLVMAKTHKRRWKMAFAAIYFTRALISLTRKVTGN-KSGALLR 62

Query: 61   SLSYVAVDVPLNDGDSTDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGLAAL 120
            SLS+V +DV +ND DSTD GE  CMA F  VD++VLSEMVREKD +CL+RFGGV+GLA L
Sbjct: 63   SLSFVTLDVQVND-DSTDSGEQPCMAGFFGVDQKVLSEMVREKDCECLNRFGGVDGLANL 122

Query: 121  LETSANGGISATDDDLIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAALSL 180
            L+T+ + GI AT+DDL  R NAFG NTY K HG+SF  FVIEAF DTTIIILLICA LSL
Sbjct: 123  LKTNPHDGICATEDDLNYRTNAFGTNTYSKRHGKSFFRFVIEAFNDTTIIILLICAVLSL 182

Query: 181  GFGIKQHGWDDGWYDGGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRAGR 240
            GFGIKQHGWDDGWYDGGSIVIAI LVVVVSA SNF QSRQFEKLS+ERDDIKI+V RAGR
Sbjct: 183  GFGIKQHGWDDGWYDGGSIVIAIVLVVVVSAASNFKQSRQFEKLSNERDDIKIEVTRAGR 242

Query: 241  RKPVSIFEVVVGDVVRLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPFLL 300
            RK VSIFE++VGD+V LKIGDQ+PADGVFL+GHSLK+DESSM GESEH EVN   NPFLL
Sbjct: 243  RKSVSIFELLVGDIVYLKIGDQIPADGVFLDGHSLKIDESSMTGESEHAEVNHEINPFLL 302

Query: 301  SGTKVSDGFGVMMVTSVGMNTAWGEMMSSIREEVNELTPLQACLNKMTAVVGKLGLTVAV 360
            SGTKV+DGFGVMMVTSVGMNTAWGEMMSSIR E+NE TPLQA LNKMTA++GKLGLTVAV
Sbjct: 303  SGTKVNDGFGVMMVTSVGMNTAWGEMMSSIRREINEETPLQARLNKMTAIIGKLGLTVAV 362

Query: 361  LVLLVLLLRYFTGSTKEFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA 420
            +VLLVLL RYFTGST EFNGSKT F+ VMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA
Sbjct: 363  VVLLVLLARYFTGSTGEFNGSKTRFDDVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA 422

Query: 421  YSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDR--YSS 480
            YSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLN+MKVTEFWIG D+I    YS+
Sbjct: 423  YSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNQMKVTEFWIGEDKIMNTDYSN 482

Query: 481  STIEAKVVEVLHQAIGLNTAGSVQRQPTSSSLLP-EIFGSPTEKAILSWAVFDLDLNLDE 540
            S I +KVVE+LHQA+GLNT GS+Q   T +S LP EIFGSPTEKAILSWAVFDLDLNLDE
Sbjct: 483  SRISSKVVELLHQAVGLNTTGSIQ---TPASSLPLEIFGSPTEKAILSWAVFDLDLNLDE 542

Query: 541  LKKHHEIIQVETFNSEKKRSGVLTRQYGERLIHTHWKGAAEMILAMCSYCYERQGTVRAI 600
            LKK HE+I VETF+SEKKRSGVLTR++G RL++THWKGAAEMILAMCSY YE+QGT RAI
Sbjct: 543  LKKQHEMILVETFSSEKKRSGVLTRRHGTRLVNTHWKGAAEMILAMCSYYYEKQGTARAI 602

Query: 601  DDEGRRRLVAIITTMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPCR 660
            D E RR+L  IIT MAEKSLRCIAFAHK+N +D   + P KLDES LTLLGIVGLKDPCR
Sbjct: 603  DGEMRRQLTEIITAMAEKSLRCIAFAHKSNEDDDIQDIPVKLDESELTLLGIVGLKDPCR 662

Query: 661  PGVRAAVESCRAAGVNIKMVTGDNLHTATAIAIECGILDLNDDLS-SEVVVEGKEFRNYT 720
            PGVR AVESCRAAGV+IKMVTGDNLHTATAIAIECGIL+ +DD + SEV+VEG +FRNYT
Sbjct: 663  PGVREAVESCRAAGVSIKMVTGDNLHTATAIAIECGILNPDDDATNSEVIVEGVKFRNYT 722

Query: 721  PEERLERVGRIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI 780
            PEERLE++G IRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI
Sbjct: 723  PEERLEKIGTIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI 782

Query: 781  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVS 840
            QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVN+AALV+NFIAAVS
Sbjct: 783  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNIAALVINFIAAVS 842

Query: 841  SGKVPLTAVQLLWVNLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQA 900
            SGKVPLTAVQLLWVNLIMDT+GALALATE PTNDLM+KTPVGRS+PLVTK+MWRNLI QA
Sbjct: 843  SGKVPLTAVQLLWVNLIMDTMGALALATELPTNDLMMKTPVGRSDPLVTKIMWRNLITQA 902

Query: 901  VYQVTVLLVLEFKGRSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSK 960
            VYQV VLLVLEFKG SIFNVNE+VKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLH+SK
Sbjct: 903  VYQVAVLLVLEFKGSSIFNVNEKVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHKSK 962

Query: 961  LFLAIIAITIAFQVIMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAME 1017
            LFLAII IT+AFQV+MVE+LGRFANT RLNW QWG+C+G+AAL+WPIGW+SKLIPVSA++
Sbjct: 963  LFLAIIVITLAFQVVMVEVLGRFANTSRLNWGQWGACLGVAALTWPIGWLSKLIPVSALQ 1019

BLAST of MC08g2325.1 vs. NCBI nr
Match: KAG6586307.1 (Calcium-transporting ATPase 12, plasma membrane-type, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1617 bits (4187), Expect = 0.0
Identity = 845/1022 (82.68%), Postives = 922/1022 (90.22%), Query Frame = 0

Query: 1    LRYRKNDEMV-IVNHEERSSMAKNHKRRWRMAFAAICFTRVLISLSKKVGGRRKSGGLLR 60
            LR RK+DE+V ++N EER  MAK HKRRW+MAF+AI FTR LISL++KV G  KSG LLR
Sbjct: 3    LRCRKSDEVVEVLNREERLVMAKTHKRRWKMAFSAIYFTRALISLTRKVTGS-KSGALLR 62

Query: 61   SLSYVAVDVPLNDGDSTDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGLAAL 120
            SLS+V +DV +ND DSTD GE  CMA F  VD++VLSEMVREKD +CL+RFGGV+GLA L
Sbjct: 63   SLSFVTLDVQVND-DSTDSGEQPCMAGFFGVDQKVLSEMVREKDCECLNRFGGVDGLANL 122

Query: 121  LETSANGGISATDDDLIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAALSL 180
            L+T+ + GI AT+DDL  R NAFG NTY K HG+SF  FVIEAF DTTIIILLICA LSL
Sbjct: 123  LKTNPHDGICATEDDLNYRTNAFGTNTYSKRHGKSFFRFVIEAFNDTTIIILLICAVLSL 182

Query: 181  GFGIKQHGWDDGWYDGGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRAGR 240
            GFGIKQHGWDDGWYDGGSIVIAI LVVVVSA SNF QSRQFEKLS+ERDDIKI+V RAGR
Sbjct: 183  GFGIKQHGWDDGWYDGGSIVIAIVLVVVVSAASNFKQSRQFEKLSNERDDIKIEVTRAGR 242

Query: 241  RKPVSIFEVVVGDVVRLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPFLL 300
            RK VSIFE++VGD+V LKIGDQ+PADGVFL+GHSLK+DESSM GESEH EVN   NPFLL
Sbjct: 243  RKSVSIFELLVGDIVYLKIGDQIPADGVFLDGHSLKIDESSMTGESEHAEVNHEINPFLL 302

Query: 301  SGTKVSDGFGVMMVTSVGMNTAWGEMMSSIREEVNELTPLQACLNKMTAVVGKLGLTVAV 360
            SGTKV+DGFGVMMVTSVGMNTAWGEMMSSIR E+NE TPLQA LNKMTA++GKLGLTVAV
Sbjct: 303  SGTKVNDGFGVMMVTSVGMNTAWGEMMSSIRREINEETPLQARLNKMTAIIGKLGLTVAV 362

Query: 361  LVLLVLLLRYFTGSTKEFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA 420
            +VLLVLL RYFTGST EFNGSKT F+ VMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA
Sbjct: 363  VVLLVLLARYFTGSTGEFNGSKTRFDDVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA 422

Query: 421  YSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDR--YSS 480
            YSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLN+MKVTEFWIG D+I    YS+
Sbjct: 423  YSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNQMKVTEFWIGEDKIMNTDYSN 482

Query: 481  STIEAKVVEVLHQAIGLNTAGSVQRQPTSSSLLP-EIFGSPTEKAILSWAVFDLDLNLDE 540
            S I +KVVE+LHQA+GLNT GS+Q   T +S LP EIFGSPTEKAILSWAVFDLDLNLDE
Sbjct: 483  SRISSKVVELLHQAVGLNTTGSIQ---TPASSLPLEIFGSPTEKAILSWAVFDLDLNLDE 542

Query: 541  LKKHHEIIQVETFNSEKKRSGVLTRQYGERLIHTHWKGAAEMILAMCSYCYERQGTVRAI 600
            LKK HE+I VETF+SEKKRSGVLTR++G RL++THWKGAAEMILAMCSY YE+QGT RAI
Sbjct: 543  LKKQHEMILVETFSSEKKRSGVLTRRHGTRLVNTHWKGAAEMILAMCSYYYEKQGTARAI 602

Query: 601  DDEGRRRLVAIITTMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPCR 660
            D E RR+L  IIT MAEK LRCIAFAHK+N +D   + P KLDES LTLLGIVGLKDPCR
Sbjct: 603  DGEMRRQLTEIITAMAEKRLRCIAFAHKSNEDDDIQDIPVKLDESELTLLGIVGLKDPCR 662

Query: 661  PGVRAAVESCRAAGVNIKMVTGDNLHTATAIAIECGILDLNDDLS-SEVVVEGKEFRNYT 720
            PGVR AVESCRAAGV+IKMVTGDNLHTATAIAIECGIL+ +DD + SEV+VEG +FRNYT
Sbjct: 663  PGVREAVESCRAAGVSIKMVTGDNLHTATAIAIECGILNPDDDATNSEVIVEGVKFRNYT 722

Query: 721  PEERLERVGRIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI 780
            PEERLE++G IRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI
Sbjct: 723  PEERLEKIGTIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI 782

Query: 781  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVS 840
            QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVN+AALV+NFIAAVS
Sbjct: 783  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNIAALVINFIAAVS 842

Query: 841  SGKVPLTAVQLLWVNLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQA 900
            SGKVPLTAVQLLWVNLIMDT+GALALATE PTNDLM KTPVGRS+PLVTK+MWRNLI QA
Sbjct: 843  SGKVPLTAVQLLWVNLIMDTMGALALATELPTNDLMTKTPVGRSDPLVTKIMWRNLITQA 902

Query: 901  VYQVTVLLVLEFKGRSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSK 960
            VYQV VLLVLEFKG SIFNVNE+VKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLH+SK
Sbjct: 903  VYQVAVLLVLEFKGSSIFNVNEKVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHKSK 962

Query: 961  LFLAIIAITIAFQVIMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAME 1017
            LFLAII IT+AFQV+MVE+LGRFANT RLNW QWG+C+GIAALSWPIGW+SKLIPVSA++
Sbjct: 963  LFLAIIVITLAFQVVMVEVLGRFANTSRLNWGQWGACLGIAALSWPIGWLSKLIPVSALQ 1019

BLAST of MC08g2325.1 vs. ExPASy TrEMBL
Match: A0A6J1CNU2 (Calcium-transporting ATPase OS=Momordica charantia OX=3673 GN=LOC111012979 PE=3 SV=1)

HSP 1 Score: 1962 bits (5082), Expect = 0.0
Identity = 1018/1019 (99.90%), Postives = 1018/1019 (99.90%), Query Frame = 0

Query: 1    LRYRKNDEMVIVNHEERSSMAKNHKRRWRMAFAAICFTRVLISLSKKVGGRRKSGGLLRS 60
            LRYRKNDEMVIVNHEERSSMAKNHKRRWRMAFAAICFTRVLISLSKKVGGRRKSGGLLRS
Sbjct: 3    LRYRKNDEMVIVNHEERSSMAKNHKRRWRMAFAAICFTRVLISLSKKVGGRRKSGGLLRS 62

Query: 61   LSYVAVDVPLNDGDSTDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGLAALL 120
            LSYVAVDVPLNDGDSTDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGLAALL
Sbjct: 63   LSYVAVDVPLNDGDSTDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGLAALL 122

Query: 121  ETSANGGISATDDDLIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAALSLG 180
            ETSANGGISATDDDLIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAALSLG
Sbjct: 123  ETSANGGISATDDDLIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAALSLG 182

Query: 181  FGIKQHGWDDGWYDGGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRAGRR 240
            FGIKQHGWDDGWYDGGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRAGRR
Sbjct: 183  FGIKQHGWDDGWYDGGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRAGRR 242

Query: 241  KPVSIFEVVVGDVVRLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPFLLS 300
            KPVSIFEVVVGDVVRLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPFLLS
Sbjct: 243  KPVSIFEVVVGDVVRLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPFLLS 302

Query: 301  GTKVSDGFGVMMVTSVGMNTAWGEMMSSIREEVNELTPLQACLNKMTAVVGKLGLTVAVL 360
            GTKVSDGFGVMMVTSVGMNTAWGEMMSSIREEVNELTPLQA LNKMTAVVGKLGLTVAVL
Sbjct: 303  GTKVSDGFGVMMVTSVGMNTAWGEMMSSIREEVNELTPLQARLNKMTAVVGKLGLTVAVL 362

Query: 361  VLLVLLLRYFTGSTKEFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLAY 420
            VLLVLLLRYFTGSTKEFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLAY
Sbjct: 363  VLLVLLLRYFTGSTKEFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLAY 422

Query: 421  SMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDRYSSSTI 480
            SMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDRYSSSTI
Sbjct: 423  SMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDRYSSSTI 482

Query: 481  EAKVVEVLHQAIGLNTAGSVQRQPTSSSLLPEIFGSPTEKAILSWAVFDLDLNLDELKKH 540
            EAKVVEVLHQAIGLNTAGSVQRQPTSSSLLPEIFGSPTEKAILSWAVFDLDLNLDELKKH
Sbjct: 483  EAKVVEVLHQAIGLNTAGSVQRQPTSSSLLPEIFGSPTEKAILSWAVFDLDLNLDELKKH 542

Query: 541  HEIIQVETFNSEKKRSGVLTRQYGERLIHTHWKGAAEMILAMCSYCYERQGTVRAIDDEG 600
            HEIIQVETFNSEKKRSGVLTRQYGERLIHTHWKGAAEMILAMCSYCYERQGTVRAIDDEG
Sbjct: 543  HEIIQVETFNSEKKRSGVLTRQYGERLIHTHWKGAAEMILAMCSYCYERQGTVRAIDDEG 602

Query: 601  RRRLVAIITTMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPCRPGVR 660
            RRRLVAIITTMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPCRPGVR
Sbjct: 603  RRRLVAIITTMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPCRPGVR 662

Query: 661  AAVESCRAAGVNIKMVTGDNLHTATAIAIECGILDLNDDLSSEVVVEGKEFRNYTPEERL 720
            AAVESCRAAGVNIKMVTGDNLHTATAIAIECGILDLNDDLSSEVVVEGKEFRNYTPEERL
Sbjct: 663  AAVESCRAAGVNIKMVTGDNLHTATAIAIECGILDLNDDLSSEVVVEGKEFRNYTPEERL 722

Query: 721  ERVGRIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGIQGTEV 780
            ERVGRIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGIQGTEV
Sbjct: 723  ERVGRIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGIQGTEV 782

Query: 781  AKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVSSGKVP 840
            AKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVSSGKVP
Sbjct: 783  AKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVSSGKVP 842

Query: 841  LTAVQLLWVNLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQAVYQVT 900
            LTAVQLLWVNLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQAVYQVT
Sbjct: 843  LTAVQLLWVNLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQAVYQVT 902

Query: 901  VLLVLEFKGRSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSKLFLAI 960
            VLLVLEFKGRSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSKLFLAI
Sbjct: 903  VLLVLEFKGRSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSKLFLAI 962

Query: 961  IAITIAFQVIMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAMEFPRR 1019
            IAITIAFQVIMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAMEFPRR
Sbjct: 963  IAITIAFQVIMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAMEFPRR 1021

BLAST of MC08g2325.1 vs. ExPASy TrEMBL
Match: A0A6J1HRB2 (Calcium-transporting ATPase OS=Cucurbita maxima OX=3661 GN=LOC111465421 PE=3 SV=1)

HSP 1 Score: 1622 bits (4201), Expect = 0.0
Identity = 848/1023 (82.89%), Postives = 923/1023 (90.22%), Query Frame = 0

Query: 1    LRYRKNDEMV-IVNHEERSSMAKNHKRRWRMAFAAICFTRVLISLSKKVGGRRKSGGLLR 60
            LR RK+DE+V ++N EER  MAK HKRRW+MAFAAI FTR LISL++KV  R KSG LL 
Sbjct: 3    LRCRKSDEVVEVLNREERLVMAKTHKRRWKMAFAAIYFTRALISLTRKVTDR-KSGALLP 62

Query: 61   SLSYVAVDVPLNDGDSTDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGLAAL 120
            SLS+V +DV +ND DSTD GE  CMA F  VD++VLSEMVREKD +CL+RFGGV+GLA L
Sbjct: 63   SLSFVTLDVQVND-DSTDSGEQPCMAGFFGVDQKVLSEMVREKDCECLNRFGGVDGLANL 122

Query: 121  LETSANGGISATDDDLIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAALSL 180
            L+T  + GI AT DDL  R NAFG NTY K HG+SF S+VIEAF DTTIIILLICA LSL
Sbjct: 123  LKTDPHDGICATQDDLTYRTNAFGTNTYSKRHGKSFFSYVIEAFNDTTIIILLICAVLSL 182

Query: 181  GFGIKQHGWDDGWYDGGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRAGR 240
            GFGIKQHGWDDGWYDGGSIVIAIFLVVVVSA SNF QSRQFEKLS+ERDDIKI+V RAGR
Sbjct: 183  GFGIKQHGWDDGWYDGGSIVIAIFLVVVVSAASNFKQSRQFEKLSNERDDIKIEVTRAGR 242

Query: 241  RKPVSIFEVVVGDVVRLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPFLL 300
            RK VSIFE++VGD+V LKIGDQ+PADGVFL+GHSLK+DESSM GESEH EVN G NPFLL
Sbjct: 243  RKSVSIFELLVGDIVYLKIGDQIPADGVFLDGHSLKIDESSMTGESEHAEVNHGINPFLL 302

Query: 301  SGTKVSDGFGVMMVTSVGMNTAWGEMMSSIREEVNELTPLQACLNKMTAVVGKLGLTVAV 360
            SGTKV+DGFGVMMVTSVGMNTAWGEMMSSIR E+NE TPLQ  LNKMTA++GKLGLTVAV
Sbjct: 303  SGTKVNDGFGVMMVTSVGMNTAWGEMMSSIRREINEETPLQGRLNKMTAIIGKLGLTVAV 362

Query: 361  LVLLVLLLRYFTGSTKEFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA 420
            +VLLVLL+RYFTGST EFNGSKT F+ VMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA
Sbjct: 363  VVLLVLLVRYFTGSTGEFNGSKTRFDDVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA 422

Query: 421  YSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDR--YSS 480
            YSMKKMMADNAMVRKLSACET+GSATTICTDKTGTLTLN+MKVTEFWIG DEI    YS+
Sbjct: 423  YSMKKMMADNAMVRKLSACETLGSATTICTDKTGTLTLNQMKVTEFWIGEDEIMNIDYSN 482

Query: 481  STIEAKVVEVLHQAIGLNTAGSVQRQPTSSSLLP-EIFGSPTEKAILSWAVFDLDLNLDE 540
            S I +KVVE+LHQA+GLNT GS+Q   T +S LP E FGSPTEKAILSWA FDLDLNLDE
Sbjct: 483  SRISSKVVELLHQAVGLNTTGSIQ---TPASSLPLETFGSPTEKAILSWAAFDLDLNLDE 542

Query: 541  LKKHHEIIQVETFNSEKKRSGVLTRQYGERLIHTHWKGAAEMILAMCSYCYERQGTVRAI 600
            LKK HEII VETF+SEKKRSGV TR++G RL++THWKGAAE+ILAMCSY YE+QGT RAI
Sbjct: 543  LKKQHEIILVETFSSEKKRSGVSTRRHGTRLVNTHWKGAAEIILAMCSYYYEKQGTARAI 602

Query: 601  DDEGRRRLVAIITTMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPCR 660
            D E RR+L  IIT MAEKSLRCIAFAHK+N +D   + PAKLDES LTLLGIVGLKDPCR
Sbjct: 603  DGEMRRQLTEIITAMAEKSLRCIAFAHKSNEDDDIRDVPAKLDESELTLLGIVGLKDPCR 662

Query: 661  PGVRAAVESCRAAGVNIKMVTGDNLHTATAIAIECGILDLNDDLS-SEVVVEGKEFRNYT 720
             GVR AVESCRAAGV+IKMVTGDNLHTATAIAIECGIL+ +DD + SEVVVEG +FRNYT
Sbjct: 663  AGVREAVESCRAAGVSIKMVTGDNLHTATAIAIECGILNPDDDATNSEVVVEGVKFRNYT 722

Query: 721  PEERLERVGRIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI 780
            PEERLE++G IRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI
Sbjct: 723  PEERLEKIGNIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI 782

Query: 781  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVS 840
            QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVN+AALV+NFIAAVS
Sbjct: 783  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNIAALVINFIAAVS 842

Query: 841  SGKVPLTAVQLLWVNLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQA 900
            SGKVPLTAVQLLWVNLIMDT+GALALATE PTNDLM KTPVGRS+PLVTK+MWRNLI QA
Sbjct: 843  SGKVPLTAVQLLWVNLIMDTMGALALATELPTNDLMTKTPVGRSDPLVTKIMWRNLITQA 902

Query: 901  VYQVTVLLVLEFKGRSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSK 960
            VYQVTVLLVLEFKG SIFNVNE+VKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLH+SK
Sbjct: 903  VYQVTVLLVLEFKGSSIFNVNEKVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHKSK 962

Query: 961  LFLAIIAITIAFQVIMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAME 1018
            LFLAII IT+AFQV+MVE+LGRFANTERLNW QWG+C+GIAALSWPIGW+SKLIPVSA++
Sbjct: 963  LFLAIIVITLAFQVVMVEVLGRFANTERLNWGQWGACLGIAALSWPIGWLSKLIPVSALQ 1020

BLAST of MC08g2325.1 vs. ExPASy TrEMBL
Match: A0A6J1FGK4 (Calcium-transporting ATPase OS=Cucurbita moschata OX=3662 GN=LOC111443998 PE=3 SV=1)

HSP 1 Score: 1620 bits (4195), Expect = 0.0
Identity = 845/1022 (82.68%), Postives = 924/1022 (90.41%), Query Frame = 0

Query: 1    LRYRKNDEMV-IVNHEERSSMAKNHKRRWRMAFAAICFTRVLISLSKKVGGRRKSGGLLR 60
            LR RK+DE+V ++N EER  MAK HKRRW+MAFAAI FTR LISL++KV G  KSG LLR
Sbjct: 3    LRCRKSDEVVEVLNREERLVMAKTHKRRWKMAFAAIYFTRALISLTRKVTGN-KSGALLR 62

Query: 61   SLSYVAVDVPLNDGDSTDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGLAAL 120
            SLS+V +DV +ND DSTD GE  CMA F  VD++VLSEMVREKD +CL+RFGGV+GLA L
Sbjct: 63   SLSFVTLDVQVND-DSTDSGEQPCMAGFFGVDQKVLSEMVREKDCECLNRFGGVDGLANL 122

Query: 121  LETSANGGISATDDDLIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAALSL 180
            L+T+ + GI AT+DDL  R NAFG NTY K HG+SF  FVIEAF DTTIIILLICA LSL
Sbjct: 123  LKTNPHDGICATEDDLNYRTNAFGTNTYSKRHGKSFFRFVIEAFNDTTIIILLICAVLSL 182

Query: 181  GFGIKQHGWDDGWYDGGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRAGR 240
            GFGIKQHGWDDGWYDGGSIVIAI LVVVVSA SNF QSRQFEKLS+ERDDIKI+V RAGR
Sbjct: 183  GFGIKQHGWDDGWYDGGSIVIAIVLVVVVSAASNFKQSRQFEKLSNERDDIKIEVTRAGR 242

Query: 241  RKPVSIFEVVVGDVVRLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPFLL 300
            RK VSIFE++VGD+V LKIGDQ+PADGVFL+GHSLK+DESSM GESEH EVN   NPFLL
Sbjct: 243  RKSVSIFELLVGDIVYLKIGDQIPADGVFLDGHSLKIDESSMTGESEHAEVNHEINPFLL 302

Query: 301  SGTKVSDGFGVMMVTSVGMNTAWGEMMSSIREEVNELTPLQACLNKMTAVVGKLGLTVAV 360
            SGTKV+DGFGVMMVTSVGMNTAWGEMMSSIR E+NE TPLQA LNKMTA++GKLGLTVAV
Sbjct: 303  SGTKVNDGFGVMMVTSVGMNTAWGEMMSSIRREINEETPLQARLNKMTAIIGKLGLTVAV 362

Query: 361  LVLLVLLLRYFTGSTKEFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA 420
            +VLLVLL RYFTGST EFNGSKT F+ VMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA
Sbjct: 363  VVLLVLLARYFTGSTGEFNGSKTRFDDVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLA 422

Query: 421  YSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDR--YSS 480
            YSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLN+MKVTEFWIG D+I    YS+
Sbjct: 423  YSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNQMKVTEFWIGEDKIMNTDYSN 482

Query: 481  STIEAKVVEVLHQAIGLNTAGSVQRQPTSSSLLP-EIFGSPTEKAILSWAVFDLDLNLDE 540
            S I +KVVE+LHQA+GLNT GS+Q   T +S LP EIFGSPTEKAILSWAVFDLDLNLDE
Sbjct: 483  SRISSKVVELLHQAVGLNTTGSIQ---TPASSLPLEIFGSPTEKAILSWAVFDLDLNLDE 542

Query: 541  LKKHHEIIQVETFNSEKKRSGVLTRQYGERLIHTHWKGAAEMILAMCSYCYERQGTVRAI 600
            LKK HE+I VETF+SEKKRSGVLTR++G RL++THWKGAAEMILAMCSY YE+QGT RAI
Sbjct: 543  LKKQHEMILVETFSSEKKRSGVLTRRHGTRLVNTHWKGAAEMILAMCSYYYEKQGTARAI 602

Query: 601  DDEGRRRLVAIITTMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPCR 660
            D E RR+L  IIT MAEKSLRCIAFAHK+N +D   + P KLDES LTLLGIVGLKDPCR
Sbjct: 603  DGEMRRQLTEIITAMAEKSLRCIAFAHKSNEDDDIQDIPVKLDESELTLLGIVGLKDPCR 662

Query: 661  PGVRAAVESCRAAGVNIKMVTGDNLHTATAIAIECGILDLNDDLS-SEVVVEGKEFRNYT 720
            PGVR AVESCRAAGV+IKMVTGDNLHTATAIAIECGIL+ +DD + SEV+VEG +FRNYT
Sbjct: 663  PGVREAVESCRAAGVSIKMVTGDNLHTATAIAIECGILNPDDDATNSEVIVEGVKFRNYT 722

Query: 721  PEERLERVGRIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI 780
            PEERLE++G IRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI
Sbjct: 723  PEERLEKIGTIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI 782

Query: 781  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVS 840
            QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVN+AALV+NFIAAVS
Sbjct: 783  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNIAALVINFIAAVS 842

Query: 841  SGKVPLTAVQLLWVNLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQA 900
            SGKVPLTAVQLLWVNLIMDT+GALALATE PTNDLM+KTPVGRS+PLVTK+MWRNLI QA
Sbjct: 843  SGKVPLTAVQLLWVNLIMDTMGALALATELPTNDLMMKTPVGRSDPLVTKIMWRNLITQA 902

Query: 901  VYQVTVLLVLEFKGRSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSK 960
            VYQV VLLVLEFKG SIFNVNE+VKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLH+SK
Sbjct: 903  VYQVAVLLVLEFKGSSIFNVNEKVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHKSK 962

Query: 961  LFLAIIAITIAFQVIMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAME 1017
            LFLAII IT+AFQV+MVE+LGRFANT RLNW QWG+C+G+AAL+WPIGW+SKLIPVSA++
Sbjct: 963  LFLAIIVITLAFQVVMVEVLGRFANTSRLNWGQWGACLGVAALTWPIGWLSKLIPVSALQ 1019

BLAST of MC08g2325.1 vs. ExPASy TrEMBL
Match: A0A0A0LLD4 (Calcium-transporting ATPase OS=Cucumis sativus OX=3659 GN=Csa_2G011490 PE=3 SV=1)

HSP 1 Score: 1587 bits (4108), Expect = 0.0
Identity = 833/1024 (81.35%), Postives = 913/1024 (89.16%), Query Frame = 0

Query: 1    LRYRKNDEMVIVNHEERSSM--AKNHKRRWRMAFAAICFTRVLISLSKKVGGRRKSGGLL 60
            LRYRKN+ + I++HE++ S+  AK HKRRWRMAFAAI FTR+LISLSKKVG R+     L
Sbjct: 4    LRYRKNEMVEIIDHEDQCSVVNAKTHKRRWRMAFAAIYFTRLLISLSKKVGHRK----FL 63

Query: 61   RSLSYVAVDVPLN--DGDSTDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGL 120
            RSLSYV VD+ L+  D D+ D  E       S VDR+VLS MVREK+ +CLDRFGGVEGL
Sbjct: 64   RSLSYVTVDMHLDYHDVDNMDSDEQR-----SYVDRKVLSMMVREKNFECLDRFGGVEGL 123

Query: 121  AALLETSANGGISATDDDLIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAA 180
            A LLET+   GI AT+DDLI+R+N+FG NTY KLHGRSF+SFVIEA  DTT+IILLICAA
Sbjct: 124  ANLLETNPEKGIVATEDDLISRKNSFGTNTYLKLHGRSFVSFVIEALNDTTMIILLICAA 183

Query: 181  LSLGFGIKQHGWDDGWYDGGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVR 240
            LSLGFGIKQHGWDDGWYDGGSIV+AIFLVV VS +SNF QSRQFEKLS+ER+DIKI+V+R
Sbjct: 184  LSLGFGIKQHGWDDGWYDGGSIVVAIFLVVGVSTISNFKQSRQFEKLSNEREDIKIEVIR 243

Query: 241  AGRRKPVSIFEVVVGDVVRLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNP 300
            AGRRKPVSIF++VVGDVV LKIGDQ+PADGVFLEGH+LKVDES M GES+ VEVNLG+NP
Sbjct: 244  AGRRKPVSIFDIVVGDVVYLKIGDQIPADGVFLEGHALKVDESQMTGESDQVEVNLGSNP 303

Query: 301  FLLSGTKVSDGFGVMMVTSVGMNTAWGEMMSSIREEVNELTPLQACLNKMTAVVGKLGLT 360
            FLLSGTKVSDGFGVMMVTSVGMNT WGEMMSSIR+EVNE TPLQA LNKMTAV+GKLGLT
Sbjct: 304  FLLSGTKVSDGFGVMMVTSVGMNTTWGEMMSSIRQEVNETTPLQARLNKMTAVIGKLGLT 363

Query: 361  VAVLVLLVLLLRYFTGSTKEFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTL 420
            VA+LVLLVLL+RYFT ST EFNGSKT FN +MNA+L MV AAVTI+VVAIPEGLPLAVTL
Sbjct: 364  VALLVLLVLLVRYFTRSTGEFNGSKTRFNDIMNAILDMVTAAVTIIVVAIPEGLPLAVTL 423

Query: 421  TLAYSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDRYS 480
            TLAYSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIG DEI    
Sbjct: 424  TLAYSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGEDEI--MD 483

Query: 481  SSTIEAKVVEVLHQAIGLNTAGSVQRQPTSSSLLPEIFGSPTEKAILSWAVFDLDLNLDE 540
                 +++VE+LHQA+GLNT GSVQR  ++SSL  EIFGSPTEKAILSWAVFDLDLNLDE
Sbjct: 484  KDLSNSRIVELLHQAVGLNTTGSVQR--STSSLPLEIFGSPTEKAILSWAVFDLDLNLDE 543

Query: 541  LKKHHEIIQVETFNSEKKRSGVLTRQYGERLIHTHWKGAAEMILAMCSYCYERQGTVRAI 600
            LKK H+IIQVETF+SEKKRSGV TR+YGE+ IHTHWKGAAEMIL MCSY Y +QGTVRAI
Sbjct: 544  LKKQHKIIQVETFSSEKKRSGVSTRRYGEKFIHTHWKGAAEMILTMCSYYYNKQGTVRAI 603

Query: 601  DDEGRRRLVAIITTMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPCR 660
            DDE R RL+A ITTMA KSLRCIAFA K N +++N E P KLDESGLTLLGIVGLKDPCR
Sbjct: 604  DDEARTRLIATITTMAGKSLRCIAFAQKQNEDNENPEVPTKLDESGLTLLGIVGLKDPCR 663

Query: 661  PGVRAAVESCRAAGVNIKMVTGDNLHTATAIAIECGILDLNDDLSS-EVVVEGKEFRNYT 720
            PGVR A+ESC+AAGV+IKMVTGDNLHTATAIA+ECGIL+ NDD ++ EVVVEG  FRNYT
Sbjct: 664  PGVREAIESCKAAGVDIKMVTGDNLHTATAIAMECGILNPNDDTNNDEVVVEGIRFRNYT 723

Query: 721  PEERLERVGRIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI 780
            PEERLE++G I+VMARSSPFDKLLMVQCLKN GHVVAVTGDGTNDAPALHEADIGLSMGI
Sbjct: 724  PEERLEKIGNIKVMARSSPFDKLLMVQCLKNLGHVVAVTGDGTNDAPALHEADIGLSMGI 783

Query: 781  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVS 840
            QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVS
Sbjct: 784  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVS 843

Query: 841  SGKVPLTAVQLLWVNLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQA 900
            SGKV LTAVQLLWVNLIMDT+GALALATE+PTNDLM K PVGR+EPLVTKVMWRNLIAQA
Sbjct: 844  SGKVSLTAVQLLWVNLIMDTMGALALATEQPTNDLMEKKPVGRTEPLVTKVMWRNLIAQA 903

Query: 901  VYQVTVLLVLEFKGRSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSK 960
            VYQVTVLLVLEFKG +IFNV  +VK TLIFNTFVLCQIFNEFNARK+EKKNIFEG+H+SK
Sbjct: 904  VYQVTVLLVLEFKGGAIFNVEGKVKGTLIFNTFVLCQIFNEFNARKMEKKNIFEGIHKSK 963

Query: 961  LFLAIIAITIAFQVIMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAME 1019
            +FL II IT+ FQV+MVELLGRFANT RLN  QWG CI IAALSWPIGW+SKLIPVSA+ 
Sbjct: 964  VFLGIIVITLGFQVVMVELLGRFANTIRLNLGQWGICIAIAALSWPIGWLSKLIPVSALH 1014

BLAST of MC08g2325.1 vs. ExPASy TrEMBL
Match: A0A1S3C230 (Calcium-transporting ATPase OS=Cucumis melo OX=3656 GN=LOC103495964 PE=3 SV=1)

HSP 1 Score: 1582 bits (4095), Expect = 0.0
Identity = 829/1024 (80.96%), Postives = 911/1024 (88.96%), Query Frame = 0

Query: 1    LRYRKNDEMVIVNHEERSSMAKNHKRRWRMAFAAICFTRVLISLSKKVGGRRKSGGLLRS 60
            L YRKND++V +++EE S   K HKRRWRMAFAAI FTRVLISLSKKVGGR+     LRS
Sbjct: 4    LSYRKNDDIVEIDYEECSVNPKTHKRRWRMAFAAIYFTRVLISLSKKVGGRK----FLRS 63

Query: 61   LSYVAVDVPLN---DGDSTDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGLA 120
            LSYV +D+ LN   D ++ D  E         VDR+VLS MVREK+ +CLDRFGGV+GLA
Sbjct: 64   LSYVTIDMHLNNHHDVENMDSDERLY------VDRKVLSMMVREKNFECLDRFGGVDGLA 123

Query: 121  ALLETSANGGISATDDDLIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAAL 180
             LLET++  GI AT+DDLI+R++ FG NTY KLHGRSF+SFVIEA  DTT+IILLICAAL
Sbjct: 124  DLLETNSEKGIVATEDDLISRKDTFGTNTYLKLHGRSFMSFVIEALNDTTMIILLICAAL 183

Query: 181  SLGFGIKQHGWDDGWYDGGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRA 240
            SLGFGIKQHGWDDGWYDGGSIV+AIFLVV VS +SNF QSRQFEKLS+ER+DIKI+V RA
Sbjct: 184  SLGFGIKQHGWDDGWYDGGSIVVAIFLVVGVSTISNFKQSRQFEKLSNEREDIKIEVTRA 243

Query: 241  GRRKPVSIFEVVVGDVVRLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPF 300
            GRRKPVSIF++VVGD+V LKIGDQ+PADGVFLEGHSLKVDES M GES+ VEVN G+NPF
Sbjct: 244  GRRKPVSIFDIVVGDIVYLKIGDQIPADGVFLEGHSLKVDESHMTGESDQVEVNHGSNPF 303

Query: 301  LLSGTKVSDGFGVMMVTSVGMNTAWGEMMSSIREEVNELTPLQACLNKMTAVVGKLGLTV 360
            LLSGTKVSDGFGVMMVTSVGMNT WGEMMSSIR+EVNE+TPLQA LNKMTAV+GKLGLTV
Sbjct: 304  LLSGTKVSDGFGVMMVTSVGMNTTWGEMMSSIRQEVNEITPLQARLNKMTAVIGKLGLTV 363

Query: 361  AVLVLLVLLLRYFTGSTKEFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLT 420
            A+LVLLVLL+RYFT ST EFNGSKT FN +MNA+L MV AAVTI+VVAIPEGLPLAVTLT
Sbjct: 364  ALLVLLVLLVRYFTRSTGEFNGSKTRFNDIMNAILDMVTAAVTILVVAIPEGLPLAVTLT 423

Query: 421  LAYSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEI-DRYS 480
            LAYSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIG DEI D++ 
Sbjct: 424  LAYSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGEDEIMDKHL 483

Query: 481  SSTIEAKVVEVLHQAIGLNTAGSVQRQPTSSSLLPEIFGSPTEKAILSWAVFDLDLNLDE 540
            S++   ++VE+LHQA+GLNT GSVQR  ++SSL  EIFGSPTEKAILSWAVFDLDLNLDE
Sbjct: 484  SNS---RIVELLHQAVGLNTTGSVQR--STSSLPLEIFGSPTEKAILSWAVFDLDLNLDE 543

Query: 541  LKKHHEIIQVETFNSEKKRSGVLTRQYGERLIHTHWKGAAEMILAMCSYCYERQGTVRAI 600
            LKK HEIIQVETF+SEKKRSGV TR+YGE+ IHTHWKGAAEMIL MCSY Y +QGTVRAI
Sbjct: 544  LKKQHEIIQVETFSSEKKRSGVSTRRYGEKFIHTHWKGAAEMILTMCSYYYNKQGTVRAI 603

Query: 601  DDEGRRRLVAIITTMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPCR 660
            DDE R+RL+  ITTMA KSLRCIAFA K N ++ N E P KLDESGLTLLGIVGLKDPCR
Sbjct: 604  DDESRKRLITTITTMAGKSLRCIAFAQKQNEDNDNPEVPTKLDESGLTLLGIVGLKDPCR 663

Query: 661  PGVRAAVESCRAAGVNIKMVTGDNLHTATAIAIECGILDLNDDLSS-EVVVEGKEFRNYT 720
            PGVR A+ESC+AAGVNIKMVTGDNLHTATAIAIECGIL+ +D+ ++ EVVVEG  FRNYT
Sbjct: 664  PGVREAIESCKAAGVNIKMVTGDNLHTATAIAIECGILNPDDNTNNDEVVVEGIRFRNYT 723

Query: 721  PEERLERVGRIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI 780
            PEERLE++  I+VMARSSPFDKLLMVQCLKN GHVVAVTGDGTNDAPALHEADIGLSMGI
Sbjct: 724  PEERLEKIDNIKVMARSSPFDKLLMVQCLKNLGHVVAVTGDGTNDAPALHEADIGLSMGI 783

Query: 781  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVS 840
            QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVS
Sbjct: 784  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVS 843

Query: 841  SGKVPLTAVQLLWVNLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQA 900
            SGKV LTAVQLLWVNLIMDT+GALALATE+PTNDLM K PVGR+EPLVTKVMWRNLIAQA
Sbjct: 844  SGKVSLTAVQLLWVNLIMDTMGALALATEQPTNDLMAKKPVGRTEPLVTKVMWRNLIAQA 903

Query: 901  VYQVTVLLVLEFKGRSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSK 960
            VYQVTVLLVLEFKG  IFNV  +VK TL+FNTFVLCQIFNEFNARK+EKKNIFEG+H+SK
Sbjct: 904  VYQVTVLLVLEFKGGVIFNVEGKVKDTLVFNTFVLCQIFNEFNARKMEKKNIFEGIHKSK 963

Query: 961  LFLAIIAITIAFQVIMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAME 1019
            +FL II IT+ FQV+MVELLGRFANT RLN  QWG CI IAALSWPIGW+SKLIPVSA+ 
Sbjct: 964  VFLGIIVITLGFQVVMVELLGRFANTRRLNLGQWGICIAIAALSWPIGWLSKLIPVSALH 1012

BLAST of MC08g2325.1 vs. TAIR 10
Match: AT3G63380.1 (ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein )

HSP 1 Score: 1219.9 bits (3155), Expect = 0.0e+00
Identity = 635/1007 (63.06%), Postives = 789/1007 (78.35%), Query Frame = 0

Query: 18   SSMAKNHKRRWRMAFAAICFTRVLISLSKKVGGRR---KSGGLLRSLSYVAVDVPLNDGD 77
            +S     +RRWR A+AAI   R ++SL K++   R   K+     SLSY A++    +G 
Sbjct: 18   TSSLNKAQRRWRFAYAAIYSMRAMLSLVKEIVPARIDPKTSDASLSLSYTALE--SGEGA 77

Query: 78   STDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGLAALLETSANGGISATDDD 137
              +   LS + A   +D+E L E+++ KD   +   GGVEG+AA L T+   GI   + +
Sbjct: 78   KINSMPLSYVPA---IDQEQLVEIMKGKDLPGIQALGGVEGVAASLRTNPTKGIHGNEQE 137

Query: 138  LIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAALSLGFGIKQHGWDDGWYD 197
            +  RR+ FG+NTY K   +  L FV EAFKD TI+ILL+CA  SLGFGIK+HG  +GWY+
Sbjct: 138  VSRRRDLFGSNTYHKPPPKGLLFFVYEAFKDLTILILLVCAIFSLGFGIKEHGIKEGWYE 197

Query: 198  GGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRAGRRKPVSIFEVVVGDVV 257
            GGSI +A+FLV+VVSA+SNF Q RQF+KLS   ++IK++V+R  RR+ +SIF+VVVGDVV
Sbjct: 198  GGSIFVAVFLVIVVSALSNFRQERQFDKLSKISNNIKVEVLRDSRRQHISIFDVVVGDVV 257

Query: 258  RLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPFLLSGTKVSDGFGVMMVT 317
             LKIGDQ+PADG+FLEGHSL+VDESSM GES+H+EV+   NPFL SGTK+ DGF  M+V 
Sbjct: 258  FLKIGDQIPADGLFLEGHSLQVDESSMTGESDHLEVDHKDNPFLFSGTKIVDGFAQMLVV 317

Query: 318  SVGMNTAWGEMMSSIREEVNELTPLQACLNKMTAVVGKLGLTVAVLVLLVLLLRYFTGST 377
            SVGM+T WG+ MSSI ++ +E TPLQ  L+ +T+ +GK+GLTVA LVL+VLL+RYFTG+T
Sbjct: 318  SVGMSTTWGQTMSSINQDSSERTPLQVRLDTLTSTIGKIGLTVAALVLVVLLVRYFTGNT 377

Query: 378  -----KEFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLAYSMKKMMADN 437
                 +E+NGSKT  ++V+N+V+ +VAAAVTIVVVAIPEGLPLAVTLTLAYSMK+MM+D 
Sbjct: 378  EKEGKREYNGSKTPVDTVVNSVVRIVAAAVTIVVVAIPEGLPLAVTLTLAYSMKRMMSDQ 437

Query: 438  AMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDRYSSSTIEAKVVEVLH 497
            AMVRKLSACETMGSAT ICTDKTGTLTLNEMKVT+FW+G + I   S+  I   V+++L+
Sbjct: 438  AMVRKLSACETMGSATVICTDKTGTLTLNEMKVTKFWLGQESIHEDSTKMISPDVLDLLY 497

Query: 498  QAIGLNTAGSVQRQPTSSSLLPEIFGSPTEKAILSWAVFDLDLNLDELKKHHEIIQVETF 557
            Q  GLNT GSV    + S+  PE  GSPTEKA+LSW V +L ++++ +K+ HE+++VETF
Sbjct: 498  QGTGLNTTGSVCVSDSGST--PEFSGSPTEKALLSWTVLNLGMDMESVKQKHEVLRVETF 557

Query: 558  NSEKKRSGVLTRQYGERLIHTHWKGAAEMILAMCSYCYERQGTVRAIDDEGRRRLVAIIT 617
            +S KKRSGVL R+  +  +H HWKGAAEM+LAMCS+ Y   G+V  +D   + R+ AII 
Sbjct: 558  SSAKKRSGVLVRRKSDNTVHVHWKGAAEMVLAMCSHYYTSTGSVDLMDSTAKSRIQAIIQ 617

Query: 618  TMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPCRPGVRAAVESCRAA 677
             MA  SLRCIAFAHK    D  LE      E GLTL+GIVGLKDPCRPGV  AVE+C+ A
Sbjct: 618  GMAASSLRCIAFAHKIASNDSVLE------EDGLTLMGIVGLKDPCRPGVSKAVETCKLA 677

Query: 678  GVNIKMVTGDNLHTATAIAIECGILDLNDDLSSEVVVEGKEFRNYTPEERLERVGRIRVM 737
            GV IKM+TGDN+ TA AIA ECGILD ND    + VVEG +FRNYT EER+++V +IRVM
Sbjct: 678  GVTIKMITGDNVFTAKAIAFECGILDHNDKDEEDAVVEGVQFRNYTDEERMQKVDKIRVM 737

Query: 738  ARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGIQGTEVAKESSDIVI 797
            ARSSP DKLLMV+CL+ +GHVVAVTGDGTNDAPAL EADIGLSMGIQGTEVAKESSDIVI
Sbjct: 738  ARSSPSDKLLMVKCLRLKGHVVAVTGDGTNDAPALKEADIGLSMGIQGTEVAKESSDIVI 797

Query: 798  LDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVSSGKVPLTAVQLLWV 857
            LDDNF SV TVLKWGRCVYNNIQKFIQFQLTVNVAALV+NFIAA+S+G+VPLTAVQLLWV
Sbjct: 798  LDDNFASVATVLKWGRCVYNNIQKFIQFQLTVNVAALVINFIAAISAGEVPLTAVQLLWV 857

Query: 858  NLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQAVYQVTVLLVLEFKG 917
            NLIMDT+GALALATERPTN+L+ + PVGR+E L+T VMWRNL+ Q++YQ+ VLL+L+FKG
Sbjct: 858  NLIMDTLGALALATERPTNELLKRKPVGRTEALITNVMWRNLLVQSLYQIAVLLILQFKG 917

Query: 918  RSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSKLFLAIIAITIAFQV 977
             SIF+V + VK TLIFNTFVLCQ+FNEFNAR++EKKN+F+GLHR++LF+ IIAITI  QV
Sbjct: 918  MSIFSVRKEVKDTLIFNTFVLCQVFNEFNAREMEKKNVFKGLHRNRLFIGIIAITIVLQV 977

Query: 978  IMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAMEF 1017
            IMVE L +FA+T RLN  QWG+CI +A+LSWPIG+ +K IPVS   F
Sbjct: 978  IMVEFLKKFADTVRLNGWQWGTCIALASLSWPIGFFTKFIPVSETPF 1011

BLAST of MC08g2325.1 vs. TAIR 10
Match: AT3G22910.1 (ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein )

HSP 1 Score: 1218.8 bits (3152), Expect = 0.0e+00
Identity = 636/1021 (62.29%), Postives = 790/1021 (77.38%), Query Frame = 0

Query: 4    RKNDEMVIVNHEERSSMAKNHKRRWRMAFAAICFTRVLISLSKKVGGRRKSGGLLRSLSY 63
            +K+   V V  E   +++K++K +W++A   +  +R L++ +K     RK G   RSLSY
Sbjct: 11   KKDKVGVEVLLELPKTLSKSNK-KWQLALIKLYCSRTLLNCAKH--AIRKPGLFPRSLSY 70

Query: 64   VAVDVPLNDGDSTDCGELSCMAAFSDVDREVLSEMVREKDSDCLDRFGGVEGLAALLETS 123
             A+D+  + GD               +D E L+++V+ K+ + L+  GG  GL + L+++
Sbjct: 71   TAIDLDHHHGDD-----------HFKIDTETLNDLVKNKNQEKLESLGGPNGLVSALKSN 130

Query: 124  ANGGISATDDDLIARRNAFGANTYPKLHGRSFLSFVIEAFKDTTIIILLICAALSLGFGI 183
               GI+   D++  RR+ FG+NTY +   +    FV+EAFKD TI+ILL CA LSLGFGI
Sbjct: 131  TRLGINEEGDEIQRRRSTFGSNTYTRQPSKGLFHFVVEAFKDLTILILLGCATLSLGFGI 190

Query: 184  KQHGWDDGWYDGGSIVIAIFLVVVVSAVSNFNQSRQFEKLSDERDDIKIDVVRAGRRKPV 243
            K+HG  +GWYDGGSI +A+FLVV VSAVSNF Q+RQF+KLS    +IKIDVVR GRR+ +
Sbjct: 191  KEHGLKEGWYDGGSIFVAVFLVVAVSAVSNFRQNRQFDKLSKVSSNIKIDVVRNGRRQEI 250

Query: 244  SIFEVVVGDVVRLKIGDQVPADGVFLEGHSLKVDESSMNGESEHVEVNLGTNPFLLSGTK 303
            SIF++VVGD+V L IGDQVPADGVF+EGH L VDESSM GES+HVEV+L  N FL SGTK
Sbjct: 251  SIFDIVVGDIVCLNIGDQVPADGVFVEGHLLHVDESSMTGESDHVEVSLTGNTFLFSGTK 310

Query: 304  VSDGFGVMMVTSVGMNTAWGEMMSSIREEVNELTPLQACLNKMTAVVGKLGLTVAVLVLL 363
            ++DGFG M VTSVGMNTAWG+MMS I  + NE TPLQ+ L+K+T+ +GK+GL VA LVLL
Sbjct: 311  IADGFGKMAVTSVGMNTAWGQMMSHISRDTNEQTPLQSRLDKLTSSIGKVGLLVAFLVLL 370

Query: 364  VLLLRYFTGSTK------EFNGSKTSFNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLT 423
            VLL+RYFTG+TK      E+NG  T  + ++NAV+ MVAAAVTI+VVAIPEGLPLAVTLT
Sbjct: 371  VLLIRYFTGTTKDESGNREYNGKTTKSDEIVNAVVKMVAAAVTIIVVAIPEGLPLAVTLT 430

Query: 424  LAYSMKKMMADNAMVRKLSACETMGSATTICTDKTGTLTLNEMKVTEFWIGADEIDRYSS 483
            LAYSMK+MM DNAMVRKLSACETMGSAT ICTDKTGTLTLN+MKVT+FW G   ++   +
Sbjct: 431  LAYSMKRMMKDNAMVRKLSACETMGSATVICTDKTGTLTLNQMKVTDFWFG---LESGKA 490

Query: 484  STIEAKVVEVLHQAIGLNTAGSVQRQPTSSSLLPEIFGSPTEKAILSWAVFDLDLNLDEL 543
            S++  +VVE+ HQ + +NT GSV +    +    E  GSPTEKAILSWAV +L++ ++++
Sbjct: 491  SSVSQRVVELFHQGVAMNTTGSVFKAKAGTEY--EFSGSPTEKAILSWAVEELEMGMEKV 550

Query: 544  KKHHEIIQVETFNSEKKRSGVLTRQYGERLIH--THWKGAAEMILAMCSYCYERQGTVRA 603
             + H+++ VE FNSEKKRSGVL ++ G    +   HWKGAAE ILAMCS   +  G VR 
Sbjct: 551  IEEHDVVHVEGFNSEKKRSGVLMKKKGVNTENNVVHWKGAAEKILAMCSTFCDGSGVVRE 610

Query: 604  IDDEGRRRLVAIITTMAEKSLRCIAFAHKTNGEDQNLETPAKLDESGLTLLGIVGLKDPC 663
            + ++ + +   II +MA KSLRCIAFA+  + ED       KL E  L+LLGI+G+KDPC
Sbjct: 611  MKEDDKIQFEKIIQSMAAKSLRCIAFAYSEDNEDNK-----KLKEEKLSLLGIIGIKDPC 670

Query: 664  RPGVRAAVESCRAAGVNIKMVTGDNLHTATAIAIECGILDLNDDLSSEVVVEGKEFRNYT 723
            RPGV+ AVE C+ AGVNIKM+TGDN+ TA AIA+ECGIL   D+++SE V+EG++FRNYT
Sbjct: 671  RPGVKKAVEDCQFAGVNIKMITGDNIFTARAIAVECGILTPEDEMNSEAVLEGEKFRNYT 730

Query: 724  PEERLERVGRIRVMARSSPFDKLLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGI 783
             EERLE+V RI+VMARSSPFDKLLMV+CLK  GHVVAVTGDGTNDAPAL EADIGLSMGI
Sbjct: 731  QEERLEKVERIKVMARSSPFDKLLMVKCLKELGHVVAVTGDGTNDAPALKEADIGLSMGI 790

Query: 784  QGTEVAKESSDIVILDDNFTSVVTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVS 843
            QGTEVAKESSDIVILDDNF SV TVLKWGRCVYNNIQKFIQFQLTVNVAALV+NF+AAVS
Sbjct: 791  QGTEVAKESSDIVILDDNFASVATVLKWGRCVYNNIQKFIQFQLTVNVAALVINFVAAVS 850

Query: 844  SGKVPLTAVQLLWVNLIMDTVGALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQA 903
            +G VPLTAVQLLWVNLIMDT+GALALATE+PTNDLM K P+GR  PL+T +MWRNL+AQA
Sbjct: 851  AGDVPLTAVQLLWVNLIMDTLGALALATEKPTNDLMKKKPIGRVAPLITNIMWRNLLAQA 910

Query: 904  VYQVTVLLVLEFKGRSIFNVNERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSK 963
             YQ++VLLVL+F+GRSIFNV E+VK+TLIFNTFVLCQ+FNEFNAR LEKKN+F+GLH+++
Sbjct: 911  FYQISVLLVLQFRGRSIFNVTEKVKNTLIFNTFVLCQVFNEFNARSLEKKNVFKGLHKNR 970

Query: 964  LFLAIIAITIAFQVIMVELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSAME 1017
            LF+ II +T+  QV+MVE L RFA+TERLN  QWG CI IAA SWPIGW+ K +PV    
Sbjct: 971  LFIGIIVVTVVLQVVMVEFLKRFADTERLNLGQWGVCIAIAAASWPIGWLVKSVPVPERH 1007

BLAST of MC08g2325.1 vs. TAIR 10
Match: AT4G29900.1 (autoinhibited Ca(2+)-ATPase 10 )

HSP 1 Score: 977.2 bits (2525), Expect = 9.8e-285
Identity = 525/939 (55.91%), Postives = 679/939 (72.31%), Query Frame = 0

Query: 90   VDREVLSEMVREKDSDCLDRFGGVEGLAALLETSANGGISATDDDLIARRNAFGANTYPK 149
            + +E +  + R+++   L   GGV GL+ LL+T+   GI   DDD++ R++AFG+NTYP+
Sbjct: 116  IGQEQIVSISRDQNIGALQELGGVRGLSDLLKTNLEKGIHGDDDDILKRKSAFGSNTYPQ 175

Query: 150  LHGRSFLSFVIEAFKDTTIIILLICAALSLGFGIKQHGWDDGWYDGGSIVIAIFLVVVVS 209
              GRSF  FV EA +D T+IIL++ A  SL  GIK  G + GWYDG SI  A+ LV+VV+
Sbjct: 176  KKGRSFWRFVWEASQDLTLIILIVAAVASLALGIKTEGIEKGWYDGISIAFAVLLVIVVT 235

Query: 210  AVSNFNQSRQFEKLSDERDDIKIDVVRAGRRKPVSIFEVVVGDVVRLKIGDQVPADGVFL 269
            A S++ QS QF+ L++E+ +I+++V R GRR  +SI+++VVGDV+ L IGDQVPADGV +
Sbjct: 236  ATSDYRQSLQFQNLNEEKRNIRLEVTRDGRRVEISIYDIVVGDVIPLNIGDQVPADGVLV 295

Query: 270  EGHSLKVDESSMNGESEHVEVNLGTNPFLLSGTKVSDGFGVMMVTSVGMNTAWGEMMSSI 329
             GHSL VDESSM GES+ V+ N   +PFL+SG KV+DG G M+VT VG+NT WG +M+S+
Sbjct: 296  AGHSLAVDESSMTGESKIVQKNSTKHPFLMSGCKVADGNGTMLVTGVGVNTEWGLLMASV 355

Query: 330  REEVNELTPLQACLNKMTAVVGKLGLTVAVLVLLVLLLRYFTGSTK------EFNGSKTS 389
             E+    TPLQ  LN +   +G +GLTVA +VL VL++RYFTG TK      +F G KT 
Sbjct: 356  SEDNGGETPLQVRLNGVATFIGIVGLTVAGVVLFVLVVRYFTGHTKNEQGGPQFIGGKTK 415

Query: 390  FNSVMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLAYSMKKMMADNAMVRKLSACETMGS 449
            F  V++ ++ +   AVTIVVVA+PEGLPLAVTLTLAYSM+KMMAD A+VR+LSACETMGS
Sbjct: 416  FEHVLDDLVEIFTVAVTIVVVAVPEGLPLAVTLTLAYSMRKMMADKALVRRLSACETMGS 475

Query: 450  ATTICTDKTGTLTLNEMKVTEFWIGADEIDR-YSSSTIEAKVVEVLHQAIGLNTAGSVQR 509
            ATTIC+DKTGTLTLNEM V E + G  ++D   SSS + +    +L + I  NT GSV R
Sbjct: 476  ATTICSDKTGTLTLNEMTVVECYAGLQKMDSPDSSSKLPSAFTSILVEGIAHNTTGSVFR 535

Query: 510  QPTSSSLLPEIFGSPTEKAILSWAVFDLDLNLDELKKHHEIIQVETFNSEKKRSGVLTRQ 569
               S S   ++ GSPTE+AIL+WA+  L ++ D LK     +Q   FNSEKKR GV  + 
Sbjct: 536  ---SESGEIQVSGSPTERAILNWAI-KLGMDFDALKSESSAVQFFPFNSEKKRGGVAVKS 595

Query: 570  YGERLIHTHWKGAAEMILAMCSYCYERQGTVRAIDDEGRRRLVAIITTMAEKSLRCIAFA 629
              +  +H HWKGAAE++L  C++  +   +   + ++    L   I  MA +SLRC+A A
Sbjct: 596  -PDSSVHIHWKGAAEIVLGSCTHYMDESESFVDMSEDKMGGLKDAIDDMAARSLRCVAIA 655

Query: 630  HKT-----NGEDQNLETPAKLDESGLTLLGIVGLKDPCRPGVRAAVESCRAAGVNIKMVT 689
             +T        D+   +  +L E  L LL IVG+KDPCRPGV+ +V  C+ AGV ++MVT
Sbjct: 656  FRTFEADKIPTDEEQLSRWELPEDDLILLAIVGIKDPCRPGVKNSVLLCQQAGVKVRMVT 715

Query: 690  GDNLHTATAIAIECGILDLNDDLSSEVVVEGKEFRNYTPEERLERVGRIRVMARSSPFDK 749
            GDN+ TA AIA+ECGIL  + D S   ++EGK FR+Y+ EER      I VM RSSP DK
Sbjct: 716  GDNIQTAKAIALECGILASDSDASEPNLIEGKVFRSYSEEERDRICEEISVMGRSSPNDK 775

Query: 750  LLMVQCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGIQGTEVAKESSDIVILDDNFTSV 809
            LL+VQ LK +GHVVAVTGDGTNDAPALHEADIGL+MGIQGTEVAKE SDI+ILDDNF SV
Sbjct: 776  LLLVQSLKRRGHVVAVTGDGTNDAPALHEADIGLAMGIQGTEVAKEKSDIIILDDNFESV 835

Query: 810  VTVLKWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVSSGKVPLTAVQLLWVNLIMDTVG 869
            V V++WGR VY NIQKFIQFQLTVNVAALV+N +AA+S+G+VPLTAVQLLWVNLIMDT+G
Sbjct: 836  VKVVRWGRSVYANIQKFIQFQLTVNVAALVINVVAAISAGEVPLTAVQLLWVNLIMDTLG 895

Query: 870  ALALATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQAVYQVTVLLVLEFKGRSIFNVN- 929
            ALALATE PT+ LM + PVGR EPL+T +MWRNL  QA+YQVTVLL+L F+G SI ++  
Sbjct: 896  ALALATEPPTDHLMDRAPVGRREPLITNIMWRNLFIQAMYQVTVLLILNFRGISILHLKS 955

Query: 930  ----ERVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSKLFLAIIAITIAFQVIMV 989
                ERVK+T+IFN FV+CQ+FNEFNARK ++ NIF G+ R+ LF+ II+ITI  QV++V
Sbjct: 956  KPNAERVKNTVIFNAFVICQVFNEFNARKPDEINIFRGVLRNHLFVGIISITIVLQVVIV 1015

Query: 990  ELLGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPV 1012
            E LG FA+T +L+W  W  CIGI ++SWP+  + KLIPV
Sbjct: 1016 EFLGTFASTTKLDWEMWLVCIGIGSISWPLAVIGKLIPV 1049

BLAST of MC08g2325.1 vs. TAIR 10
Match: AT5G57110.1 (autoinhibited Ca2+ -ATPase, isoform 8 )

HSP 1 Score: 976.5 bits (2523), Expect = 1.7e-284
Identity = 522/939 (55.59%), Postives = 669/939 (71.25%), Query Frame = 0

Query: 93   EVLSEMVREKDSDCLDRFGGVEGLAALLETSANGGISATDDDLIARRNAFGANTYPKLHG 152
            E L  M ++ +S  L+++GG +GLA LL+T+   GIS  DDDL+ R+  +G+NTYP+  G
Sbjct: 119  EQLVIMSKDHNSGALEQYGGTQGLANLLKTNPEKGISGDDDDLLKRKTIYGSNTYPRKKG 178

Query: 153  RSFLSFVIEAFKDTTIIILLICAALSLGFGIKQHGWDDGWYDGGSIVIAIFLVVVVSAVS 212
            + FL F+ +A  D T+IIL++ A  SL  GIK  G  +GWYDGGSI  A+ LV+VV+AVS
Sbjct: 179  KGFLRFLWDACHDLTLIILMVAAVASLALGIKTEGIKEGWYDGGSIAFAVILVIVVTAVS 238

Query: 213  NFNQSRQFEKLSDERDDIKIDVVRAGRRKPVSIFEVVVGDVVRLKIGDQVPADGVFLEGH 272
            ++ QS QF+ L+DE+ +I ++V+R GRR  +SI+++VVGDV+ L IG+QVPADGV + GH
Sbjct: 239  DYKQSLQFQNLNDEKRNIHLEVLRGGRRVEISIYDIVVGDVIPLNIGNQVPADGVLISGH 298

Query: 273  SLKVDESSMNGESEHVEVNLGTNPFLLSGTKVSDGFGVMMVTSVGMNTAWGEMMSSIREE 332
            SL +DESSM GES+ V  +   +PFL+SG KV+DG G M+VT VG+NT WG +M+SI E+
Sbjct: 299  SLALDESSMTGESKIVNKDANKDPFLMSGCKVADGNGSMLVTGVGVNTEWGLLMASISED 358

Query: 333  VNELTPLQACLNKMTAVVGKLGLTVAVLVLLVLLLRYFTGSTKEFNG------SKTSFNS 392
              E TPLQ  LN +   +G +GL VA  VL++LL RYFTG TK+ NG       KT    
Sbjct: 359  NGEETPLQVRLNGVATFIGSIGLAVAAAVLVILLTRYFTGHTKDNNGGPQFVKGKTKVGH 418

Query: 393  VMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLAYSMKKMMADNAMVRKLSACETMGSATT 452
            V++ V+ ++  AVTIVVVA+PEGLPLAVTLTLAYSM+KMMAD A+VR+LSACETMGSATT
Sbjct: 419  VIDDVVKVLTVAVTIVVVAVPEGLPLAVTLTLAYSMRKMMADKALVRRLSACETMGSATT 478

Query: 453  ICTDKTGTLTLNEMKVTEFWIGADEIDRYSSSTIEAKVVEVLHQAIGLNTAGSVQRQPTS 512
            IC+DKTGTLTLN+M V E + G  + D   +  + A +  ++ + I  NT GS+      
Sbjct: 479  ICSDKTGTLTLNQMTVVESYAGGKKTD---TEQLPATITSLVVEGISQNTTGSIFVPEGG 538

Query: 513  SSLLPEIFGSPTEKAILSWAVFDLDLNLDELKKHHEIIQVETFNSEKKRSGVLTRQYGER 572
              L  E  GSPTEKAIL W V  L +N +  +    I+    FNSEKKR GV  +   + 
Sbjct: 539  GDL--EYSGSPTEKAILGWGV-KLGMNFETARSQSSILHAFPFNSEKKRGGVAVKT-ADG 598

Query: 573  LIHTHWKGAAEMILAMCSYCYERQGTVRAIDDEGRRRLVAIITTMAEKSLRCIAFAHKTN 632
             +H HWKGA+E++LA C    +  G V  + D+        I  MA ++LRC+A A +T 
Sbjct: 599  EVHVHWKGASEIVLASCRSYIDEDGNVAPMTDDKASFFKNGINDMAGRTLRCVALAFRTY 658

Query: 633  GEDQNLETPAKLD-----ESGLTLLGIVGLKDPCRPGVRAAVESCRAAGVNIKMVTGDNL 692
             E + + T  +L      E  L LL IVG+KDPCRPGV+ +V  C+ AGV ++MVTGDN+
Sbjct: 659  -EAEKVPTGEELSKWVLPEDDLILLAIVGIKDPCRPGVKDSVVLCQNAGVKVRMVTGDNV 718

Query: 693  HTATAIAIECGILDLNDDLSSEVVVEGKEFRNYTPEERLERVGRIRVMARSSPFDKLLMV 752
             TA AIA+ECGIL  + DLS   ++EGK FR  T  ER +   +I VM RSSP DKLL+V
Sbjct: 719  QTARAIALECGILSSDADLSEPTLIEGKSFREMTDAERDKISDKISVMGRSSPNDKLLLV 778

Query: 753  QCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGIQGTEVAKESSDIVILDDNFTSVVTVL 812
            Q L+ QGHVVAVTGDGTNDAPALHEADIGL+MGI GTEVAKESSDI+ILDDNF SVV V+
Sbjct: 779  QSLRRQGHVVAVTGDGTNDAPALHEADIGLAMGIAGTEVAKESSDIIILDDNFASVVKVV 838

Query: 813  KWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVSSGKVPLTAVQLLWVNLIMDTVGALAL 872
            +WGR VY NIQKFIQFQLTVNVAALV+N +AA+SSG VPLTAVQLLWVNLIMDT+GALAL
Sbjct: 839  RWGRSVYANIQKFIQFQLTVNVAALVINVVAAISSGDVPLTAVQLLWVNLIMDTLGALAL 898

Query: 873  ATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQAVYQVTVLLVLEFKGRSIFNVNE---- 932
            ATE PT+ LM + PVGR EPL+T +MWRNL+ QA+YQV+VLL L F+G SI  +      
Sbjct: 899  ATEPPTDHLMGRPPVGRKEPLITNIMWRNLLIQAIYQVSVLLTLNFRGISILGLEHEVHE 958

Query: 933  ---RVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSKLFLAIIAITIAFQVIMVEL 992
               RVK+T+IFN FVLCQ FNEFNARK ++KNIF+G+ +++LF+ II IT+  QVI+VE 
Sbjct: 959  HATRVKNTIIFNAFVLCQAFNEFNARKPDEKNIFKGVIKNRLFMGIIVITLVLQVIIVEF 1018

Query: 993  LGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSA 1014
            LG+FA+T +LNW QW  C+GI  +SWP+  V K IPV A
Sbjct: 1019 LGKFASTTKLNWKQWLICVGIGVISWPLALVGKFIPVPA 1049

BLAST of MC08g2325.1 vs. TAIR 10
Match: AT5G57110.2 (autoinhibited Ca2+ -ATPase, isoform 8 )

HSP 1 Score: 976.5 bits (2523), Expect = 1.7e-284
Identity = 522/939 (55.59%), Postives = 669/939 (71.25%), Query Frame = 0

Query: 93   EVLSEMVREKDSDCLDRFGGVEGLAALLETSANGGISATDDDLIARRNAFGANTYPKLHG 152
            E L  M ++ +S  L+++GG +GLA LL+T+   GIS  DDDL+ R+  +G+NTYP+  G
Sbjct: 119  EQLVIMSKDHNSGALEQYGGTQGLANLLKTNPEKGISGDDDDLLKRKTIYGSNTYPRKKG 178

Query: 153  RSFLSFVIEAFKDTTIIILLICAALSLGFGIKQHGWDDGWYDGGSIVIAIFLVVVVSAVS 212
            + FL F+ +A  D T+IIL++ A  SL  GIK  G  +GWYDGGSI  A+ LV+VV+AVS
Sbjct: 179  KGFLRFLWDACHDLTLIILMVAAVASLALGIKTEGIKEGWYDGGSIAFAVILVIVVTAVS 238

Query: 213  NFNQSRQFEKLSDERDDIKIDVVRAGRRKPVSIFEVVVGDVVRLKIGDQVPADGVFLEGH 272
            ++ QS QF+ L+DE+ +I ++V+R GRR  +SI+++VVGDV+ L IG+QVPADGV + GH
Sbjct: 239  DYKQSLQFQNLNDEKRNIHLEVLRGGRRVEISIYDIVVGDVIPLNIGNQVPADGVLISGH 298

Query: 273  SLKVDESSMNGESEHVEVNLGTNPFLLSGTKVSDGFGVMMVTSVGMNTAWGEMMSSIREE 332
            SL +DESSM GES+ V  +   +PFL+SG KV+DG G M+VT VG+NT WG +M+SI E+
Sbjct: 299  SLALDESSMTGESKIVNKDANKDPFLMSGCKVADGNGSMLVTGVGVNTEWGLLMASISED 358

Query: 333  VNELTPLQACLNKMTAVVGKLGLTVAVLVLLVLLLRYFTGSTKEFNG------SKTSFNS 392
              E TPLQ  LN +   +G +GL VA  VL++LL RYFTG TK+ NG       KT    
Sbjct: 359  NGEETPLQVRLNGVATFIGSIGLAVAAAVLVILLTRYFTGHTKDNNGGPQFVKGKTKVGH 418

Query: 393  VMNAVLGMVAAAVTIVVVAIPEGLPLAVTLTLAYSMKKMMADNAMVRKLSACETMGSATT 452
            V++ V+ ++  AVTIVVVA+PEGLPLAVTLTLAYSM+KMMAD A+VR+LSACETMGSATT
Sbjct: 419  VIDDVVKVLTVAVTIVVVAVPEGLPLAVTLTLAYSMRKMMADKALVRRLSACETMGSATT 478

Query: 453  ICTDKTGTLTLNEMKVTEFWIGADEIDRYSSSTIEAKVVEVLHQAIGLNTAGSVQRQPTS 512
            IC+DKTGTLTLN+M V E + G  + D   +  + A +  ++ + I  NT GS+      
Sbjct: 479  ICSDKTGTLTLNQMTVVESYAGGKKTD---TEQLPATITSLVVEGISQNTTGSIFVPEGG 538

Query: 513  SSLLPEIFGSPTEKAILSWAVFDLDLNLDELKKHHEIIQVETFNSEKKRSGVLTRQYGER 572
              L  E  GSPTEKAIL W V  L +N +  +    I+    FNSEKKR GV  +   + 
Sbjct: 539  GDL--EYSGSPTEKAILGWGV-KLGMNFETARSQSSILHAFPFNSEKKRGGVAVKT-ADG 598

Query: 573  LIHTHWKGAAEMILAMCSYCYERQGTVRAIDDEGRRRLVAIITTMAEKSLRCIAFAHKTN 632
             +H HWKGA+E++LA C    +  G V  + D+        I  MA ++LRC+A A +T 
Sbjct: 599  EVHVHWKGASEIVLASCRSYIDEDGNVAPMTDDKASFFKNGINDMAGRTLRCVALAFRTY 658

Query: 633  GEDQNLETPAKLD-----ESGLTLLGIVGLKDPCRPGVRAAVESCRAAGVNIKMVTGDNL 692
             E + + T  +L      E  L LL IVG+KDPCRPGV+ +V  C+ AGV ++MVTGDN+
Sbjct: 659  -EAEKVPTGEELSKWVLPEDDLILLAIVGIKDPCRPGVKDSVVLCQNAGVKVRMVTGDNV 718

Query: 693  HTATAIAIECGILDLNDDLSSEVVVEGKEFRNYTPEERLERVGRIRVMARSSPFDKLLMV 752
             TA AIA+ECGIL  + DLS   ++EGK FR  T  ER +   +I VM RSSP DKLL+V
Sbjct: 719  QTARAIALECGILSSDADLSEPTLIEGKSFREMTDAERDKISDKISVMGRSSPNDKLLLV 778

Query: 753  QCLKNQGHVVAVTGDGTNDAPALHEADIGLSMGIQGTEVAKESSDIVILDDNFTSVVTVL 812
            Q L+ QGHVVAVTGDGTNDAPALHEADIGL+MGI GTEVAKESSDI+ILDDNF SVV V+
Sbjct: 779  QSLRRQGHVVAVTGDGTNDAPALHEADIGLAMGIAGTEVAKESSDIIILDDNFASVVKVV 838

Query: 813  KWGRCVYNNIQKFIQFQLTVNVAALVVNFIAAVSSGKVPLTAVQLLWVNLIMDTVGALAL 872
            +WGR VY NIQKFIQFQLTVNVAALV+N +AA+SSG VPLTAVQLLWVNLIMDT+GALAL
Sbjct: 839  RWGRSVYANIQKFIQFQLTVNVAALVINVVAAISSGDVPLTAVQLLWVNLIMDTLGALAL 898

Query: 873  ATERPTNDLMLKTPVGRSEPLVTKVMWRNLIAQAVYQVTVLLVLEFKGRSIFNVNE---- 932
            ATE PT+ LM + PVGR EPL+T +MWRNL+ QA+YQV+VLL L F+G SI  +      
Sbjct: 899  ATEPPTDHLMGRPPVGRKEPLITNIMWRNLLIQAIYQVSVLLTLNFRGISILGLEHEVHE 958

Query: 933  ---RVKSTLIFNTFVLCQIFNEFNARKLEKKNIFEGLHRSKLFLAIIAITIAFQVIMVEL 992
               RVK+T+IFN FVLCQ FNEFNARK ++KNIF+G+ +++LF+ II IT+  QVI+VE 
Sbjct: 959  HATRVKNTIIFNAFVLCQAFNEFNARKPDEKNIFKGVIKNRLFMGIIVITLVLQVIIVEF 1018

Query: 993  LGRFANTERLNWSQWGSCIGIAALSWPIGWVSKLIPVSA 1014
            LG+FA+T +LNW QW  C+GI  +SWP+  V K IPV A
Sbjct: 1019 LGKFASTTKLNWKQWLICVGIGVISWPLALVGKFIPVPA 1049