MC05g_new0034.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC05g_new0034.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	glyoxylate/hydroxypyruvate reductase HPR3-like
Location	MC05: 2325607 .. 2326858 (+)
Sequence length	969
RNA-Seq Expression	MC05g_new0034.1
Synteny	MC05g_new0034.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCCTCAGCTTCCTGAAGTTTTGGCTTTAGGTTCTCCCTTGGTATTCTCACTTCTGGAATCCCAATTCCCAAACAGATTCCATTTCCTCAAACCATGGCTTTCCAAACTCCCACTCCTCCAATTCCTCACCTCTTATGCCCAATCCACACAGGCCCTCATCATCCGTGGTGGTGGCTCTATCAAGCTCACTCCCGCCCTTCTCCACTGCCTCCCCTCGCTCAAGCTCGTCGTCACCTCCAGCGTCGGTGTCGATCACCTCGACTTGCCCGAATTGCGCCGCCGCGGGGTCGCCGTTGCCAATGCTGGAAACATCTTTTCCGACGATGTCGCCGATCTGGCGGTGGGATTACTCATCGACGTTCTTAGGAAAGTGTCGGCCGCGGACCGATTCCTGAGGCAAGGGCTCTGGCCTACAAAAGGAGAGTTTCCTCTCGGGTTAAAGGTACAGTGCATTTTGTCTAATAGCCGTTTCCCTTTAAATCATGATATTTAGGGCGGTTTGGTTTCTTTCAACTAAATATAGTTCTTGTTTCTACTCCCGGTAACATTTAGAAACAACCCAAACAAATTTCTGCTTTCTTGTTCTTATTTCCAATTTGTTCCAAAATATAAATAAAAGATTGTTTTAATCACACTTGAATTTATGGATGTTGAGACCATAGAAACAAAAATGTTATCAAACGGACCGTTACTTAATTTAGGTTCTTCTTGAAAAAATTTGACAGTTGAGCCAGAGACGAATCGGGATTATTGGATTGGGGAAGATAGGGAGTGAAGTTGCGAAAAGGCTGGAGGGTTTTGGGTGCAGAATCTCATACACTTCAAGGACCAAAAAGCCATTAGTTCCATACTCATACTATTCCAATGTACATGAACTGGCAGCAGAATGTGAAGCACTGATAATCTGTTGTGGGTTAAATGGAGAAACCCACCATATGATCGACAGAGAGGTGATGGTTGCATTGGGAAAAGATGGAGTGATAATCAACGTCGGGCGTGGGGGAATCATTGATGAGAAGGAGATGATTGAATGTTTGATTGAGGGGAAGATTGGGGGAGCTGGGCTTGATGTGTTTGAGAATGAACCTGAAATTCCCCAACAGCTTTGCAGTCTTGAAAATGTTGTGTTGTCACCACATGCAGCTGTGGTAACACATGAATCTTTTGTGGGATTGTGTGAGTTGGTGGTGGACAACTTGGAAGCTTTCTTCTCAAATAAGCCTCTGGTGTCTCTCTTTGTGGATTAG

mRNA sequence

ATGGATCCTCAGCTTCCTGAAGTTTTGGCTTTAGGTTCTCCCTTGGTATTCTCACTTCTGGAATCCCAATTCCCAAACAGATTCCATTTCCTCAAACCATGGCTTTCCAAACTCCCACTCCTCCAATTCCTCACCTCTTATGCCCAATCCACACAGGCCCTCATCATCCGTGGTGGTGGCTCTATCAAGCTCACTCCCGCCCTTCTCCACTGCCTCCCCTCGCTCAAGCTCGTCGTCACCTCCAGCGTCGGTGTCGATCACCTCGACTTGCCCGAATTGCGCCGCCGCGGGGTCGCCGTTGCCAATGCTGGAAACATCTTTTCCGACGATGTCGCCGATCTGGCGGTGGGATTACTCATCGACGTTCTTAGGAAAGTGTCGGCCGCGGACCGATTCCTGAGGCAAGGGCTCTGGCCTACAAAAGGAGAGTTTCCTCTCGGGTTAAAGTTGAGCCAGAGACGAATCGGGATTATTGGATTGGGGAAGATAGGGAGTGAAGTTGCGAAAAGGCTGGAGGGTTTTGGGTGCAGAATCTCATACACTTCAAGGACCAAAAAGCCATTAGTTCCATACTCATACTATTCCAATGTACATGAACTGGCAGCAGAATGTGAAGCACTGATAATCTGTTGTGGGTTAAATGGAGAAACCCACCATATGATCGACAGAGAGGTGATGGTTGCATTGGGAAAAGATGGAGTGATAATCAACGTCGGGCGTGGGGGAATCATTGATGAGAAGGAGATGATTGAATGTTTGATTGAGGGGAAGATTGGGGGAGCTGGGCTTGATGTGTTTGAGAATGAACCTGAAATTCCCCAACAGCTTTGCAGTCTTGAAAATGTTGTGTTGTCACCACATGCAGCTGTGGTAACACATGAATCTTTTGTGGGATTGTGTGAGTTGGTGGTGGACAACTTGGAAGCTTTCTTCTCAAATAAGCCTCTGGTGTCTCTCTTTGTGGATTAG

Coding sequence (CDS)

ATGGATCCTCAGCTTCCTGAAGTTTTGGCTTTAGGTTCTCCCTTGGTATTCTCACTTCTGGAATCCCAATTCCCAAACAGATTCCATTTCCTCAAACCATGGCTTTCCAAACTCCCACTCCTCCAATTCCTCACCTCTTATGCCCAATCCACACAGGCCCTCATCATCCGTGGTGGTGGCTCTATCAAGCTCACTCCCGCCCTTCTCCACTGCCTCCCCTCGCTCAAGCTCGTCGTCACCTCCAGCGTCGGTGTCGATCACCTCGACTTGCCCGAATTGCGCCGCCGCGGGGTCGCCGTTGCCAATGCTGGAAACATCTTTTCCGACGATGTCGCCGATCTGGCGGTGGGATTACTCATCGACGTTCTTAGGAAAGTGTCGGCCGCGGACCGATTCCTGAGGCAAGGGCTCTGGCCTACAAAAGGAGAGTTTCCTCTCGGGTTAAAGTTGAGCCAGAGACGAATCGGGATTATTGGATTGGGGAAGATAGGGAGTGAAGTTGCGAAAAGGCTGGAGGGTTTTGGGTGCAGAATCTCATACACTTCAAGGACCAAAAAGCCATTAGTTCCATACTCATACTATTCCAATGTACATGAACTGGCAGCAGAATGTGAAGCACTGATAATCTGTTGTGGGTTAAATGGAGAAACCCACCATATGATCGACAGAGAGGTGATGGTTGCATTGGGAAAAGATGGAGTGATAATCAACGTCGGGCGTGGGGGAATCATTGATGAGAAGGAGATGATTGAATGTTTGATTGAGGGGAAGATTGGGGGAGCTGGGCTTGATGTGTTTGAGAATGAACCTGAAATTCCCCAACAGCTTTGCAGTCTTGAAAATGTTGTGTTGTCACCACATGCAGCTGTGGTAACACATGAATCTTTTGTGGGATTGTGTGAGTTGGTGGTGGACAACTTGGAAGCTTTCTTCTCAAATAAGCCTCTGGTGTCTCTCTTTGTGGATTAG

Protein sequence

MDPQLPEVLALGSPLVFSLLESQFPNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGSIKLTPALLHCLPSLKLVVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLIDVLRKVSAADRFLRQGLWPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYTSRTKKPLVPYSYYSNVHELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRGGIIDEKEMIECLIEGKIGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCELVVDNLEAFFSNKPLVSLFVD

Homology

[Close]

BLAST of MC05g_new0034.1 vs. ExPASy Swiss-Prot
Match: Q9LE33 (Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR3 PE=2 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 7.4e-90
Identity = 166/314 (52.87%), Postives = 220/314 (70.06%), Query Frame = 0


Query: 6   PEVLALGSPLVFSLLESQFPNRFH-FLKPWLSKLPLLQFLTSYAQSTQALIIRGGGSIKL 65
           P V+ L  P   + ++      F   +    S   L  F   +A S +A +I   G + +
Sbjct: 7   PPVVLLHRPPSLTFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVI--SGRLPV 66

Query: 66  TPALLHCLPSLKLVVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLIDVLR 125
           T  LL  LPSL+++V +SVG+DH+DL   +RRG+ + NAGN FSDDVAD AVGLLI VLR
Sbjct: 67  TDELLSHLPSLQILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLLISVLR 126

Query: 126 KVSAADRFLRQGLWPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYTSRT 185
           ++ AADR++R G W   G+F LG K+S +R+GI+GLG IGS VAKRLE FGC ISY SR+
Sbjct: 127 RIPAADRYVRSGNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVISYNSRS 186

Query: 186 KKPLVPYSYYSNVHELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRGGII 245
           +K   PY YYS++  LA   + L++CC L  ETHH+++REVM  LGKDGV+INVGRG +I
Sbjct: 187 QKQSSPYRYYSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVGRGKLI 246

Query: 246 DEKEMIECLIEGKIGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCELVV 305
           DEKEM++CL++G IGGAGLDVFENEP +PQ+L  L+NVVLSPH AV T  S   + ++ +
Sbjct: 247 DEKEMVKCLVDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNVAQIAL 306

Query: 306 DNLEAFFSNKPLVS 319
            NL+AFFSN+PL+S
Sbjct: 307 ANLKAFFSNRPLLS 318

[Close]

BLAST of MC05g_new0034.1 vs. ExPASy Swiss-Prot
Match: Q65CJ7 (Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR PE=1 SV=2)

HSP 1 Score: 270.0 bits (689), Expect = 3.4e-71
Identity = 139/299 (46.49%), Postives = 199/299 (66.56%), Query Frame = 0


Query: 20  LESQFPNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGSIKLTPALLHCLPSLKLVV 79
           LE +   RF   + W ++     FL   A+S +A++  G  +      L+  LP L++V 
Sbjct: 16  LEQELDKRFKLFRYW-TQPAQRDFLALQAESIRAVV--GNSNAGADAELIDALPKLEIVS 75

Query: 80  TSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLIDVLRKVSAADRFLRQGLWP 139
           + SVG+D +DL +   +GV V N  ++ +DDVADLA+GL++ VLR++   D+++R+G W 
Sbjct: 76  SFSVGLDKVDLIKCEEKGVRVTNTPDVLTDDVADLAIGLILAVLRRICECDKYVRRGAWK 135

Query: 140 TKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYTSRTKKPLVPYSYYSNVHE 199
             G+F L  K S +R+GIIGLG+IG  VA+R E F C ISY SR+KKP   Y+YY +V E
Sbjct: 136 F-GDFKLTTKFSGKRVGIIGLGRIGLAVAERAEAFDCPISYFSRSKKPNTNYTYYGSVVE 195

Query: 200 LAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRGGIIDEKEMIECLIEGKIG 259
           LA+  + L++ C L  ET H+I+REV+ ALG  GV+IN+GRG  +DE E++  L+EG++G
Sbjct: 196 LASNSDILVVACPLTPETTHIINREVIDALGPKGVLINIGRGPHVDEPELVSALVEGRLG 255

Query: 260 GAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCELVVDNLEAFFSNKPLVS 319
           GAGLDVFE EPE+P++L  LENVVL PH    T E+   + +LVV NLEA FS KPL++
Sbjct: 256 GAGLDVFEREPEVPEKLFGLENVVLLPHVGSGTVETRKVMADLVVGNLEAHFSGKPLLT 310

[Close]

BLAST of MC05g_new0034.1 vs. ExPASy Swiss-Prot
Match: A5CAL1 (Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN=2KGR PE=1 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 2.2e-70
Identity = 133/300 (44.33%), Postives = 198/300 (66.00%), Query Frame = 0


Query: 20  LESQFPNRFHFLKPWLSKLPLLQFL-TSYAQSTQALIIRGGGSIKLTPALLHCLPSLKLV 79
           LE +   RF   + W    P    L   ++ S +A++  G   I     ++  LP +++V
Sbjct: 16  LEQELDKRFKLFRFW--DFPSANDLFREHSNSIRAVV--GNSFIGADAQMIEALPKMEIV 75

Query: 80  VTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLIDVLRKVSAADRFLRQGLW 139
            + SVG+D +DL   + +G+ V N  ++ ++DVADLA+ L++  LR++  +DR++R G W
Sbjct: 76  SSFSVGLDKIDLVRCKEKGIRVTNTPDVLTEDVADLALALILATLRRICESDRYVRSGSW 135

Query: 140 PTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYTSRTKKPLVPYSYYSNVH 199
             KG+F L  K + + +GIIGLG+IGS +AKR EGF C ISY SRT+KP   Y YY +V 
Sbjct: 136 -KKGDFKLTTKFTGKSVGIIGLGRIGSAIAKRAEGFSCPISYHSRTEKPGTNYKYYPSVV 195

Query: 200 ELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRGGIIDEKEMIECLIEGKI 259
           ELA+ C+ L++ C L  ET H+I+REV+ ALG  GV+IN+GRG  +DE E++  L+EG++
Sbjct: 196 ELASNCQILVVACALTPETRHIINREVINALGPKGVVINIGRGLHVDEPELVSALVEGRL 255

Query: 260 GGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCELVVDNLEAFFSNKPLVS 319
           GGAGLDVFENEP +P++L +++NVVL PH    T E+   + +LV+ NLEA F NKPL++
Sbjct: 256 GGAGLDVFENEPNVPEELLAMDNVVLLPHVGSGTVETRKDMADLVLGNLEAHFLNKPLLT 310

[Close]

BLAST of MC05g_new0034.1 vs. ExPASy Swiss-Prot
Match: Q9CA90 (Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=HPR2 PE=1 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 4.2e-69
Identity = 135/301 (44.85%), Postives = 199/301 (66.11%), Query Frame = 0


Query: 18  SLLESQFPNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGSIKLTPALLHCLPSLKL 77
           S LE++   RF+ L+ W S    +  L ++  S +A++  G  S      L+  LP+L++
Sbjct: 14  SYLENELEKRFNLLRFWTSPEKSV-LLETHRNSIRAVV--GNASAGADAQLISDLPNLEI 73

Query: 78  VVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLIDVLRKVSAADRFLRQGL 137
           V + SVG+D +DL + + +G+ V N  ++ ++DVADLA+GL++ +LR++   DR++R G 
Sbjct: 74  VSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLCECDRYVRSGK 133

Query: 138 WPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYTSRTKKPLVPYSYYSNV 197
           W  +GEF L  K S + +GIIGLG+IG+ +AKR E F C I+Y SRT KP V Y YY  V
Sbjct: 134 W-KQGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIKPDVAYKYYPTV 193

Query: 198 HELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRGGIIDEKEMIECLIEGK 257
            +LA   + L++ C L  +T H++DR+VM ALG  GV+IN+GRG  +DE+E+I+ L EG+
Sbjct: 194 VDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQELIKALTEGR 253

Query: 258 IGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCELVVDNLEAFFSNKPLV 317
           +GGA LDVFE EP +P++L  LENVVL PH    T E+   + +LVV NLEA FS K L+
Sbjct: 254 LGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNLEAHFSGKSLL 310

Query: 318 S 319
           +
Sbjct: 314 T 310

[Close]

BLAST of MC05g_new0034.1 vs. ExPASy Swiss-Prot
Match: Q5FTU6 (2-ketogluconate reductase OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=GOX0417 PE=1 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 3.6e-52
Identity = 114/315 (36.19%), Postives = 180/315 (57.14%), Query Frame = 0


Query: 6   PEVLALGS--PLVFSLLESQFPNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGSIK 65
           P++L +    P++   LE  F      L P+ S    L+ L + A + + +   GG  + 
Sbjct: 5   PDILTIDPLVPVMKERLEKSFT-----LHPYTS----LENLKNIAPAIRGITTGGGSGV- 64

Query: 66  LTPALLHCLPSLKLVVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLIDVL 125
               ++  LP+L+++  + VG D ++L E RRR + VA   N  +DDVAD+AV L++ V+
Sbjct: 65  -PSEIMDALPNLEVISVNGVGTDRINLDEARRRNIGVAITQNTLTDDVADMAVALMMAVM 124

Query: 126 RKVSAADRFLRQGLWPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYTSR 185
           R +   D F+R G WP+    PLG  L+++++GI G G IG  +AKR+  FG  ++Y + 
Sbjct: 125 RSIVTNDAFVRAGKWPS-ATAPLGRSLTRKKVGIAGFGHIGQAIAKRVSAFGMEVAYFNS 184

Query: 186 TKKPLVPYSYYSNVHELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRGGI 245
             +P     +  ++  LA  C+ LI+       + +MIDR+ + ALGKDG ++N+ RG +
Sbjct: 185 HARPESTCHFEPDLKALATWCDVLILAVSGGPRSANMIDRDTLDALGKDGFLVNIARGTV 244

Query: 246 IDEKEMIECLIEGKIGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCELV 305
           +DE  ++  L E +I GAGLDVF+NEP I     SL N VL  H A  T E+   +  LV
Sbjct: 245 VDEAALLSALQEKRIAGAGLDVFQNEPNINPAFLSLPNTVLQAHQASATVETRTTMANLV 304

Query: 306 VDNLEAFFSNKPLVS 319
           VDNL A+F++K L++
Sbjct: 305 VDNLIAYFTDKTLLT 307

[Close]

BLAST of MC05g_new0034.1 vs. NCBI nr
Match: XP_022147257.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Momordica charantia])

HSP 1 Score: 513 bits (1321), Expect = 4.79e-181
Identity = 257/317 (81.07%), Postives = 282/317 (88.96%), Query Frame = 0


Query: 2   DPQLPEVLALGSPLVFSLLESQFPNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGS 61
           D  LP+VL LG P VFS LESQFPNRFHFLKPWLSKLPL QFLTSYAQ  QALII  GGS
Sbjct: 7   DQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALII-PGGS 66

Query: 62  IKLTPALLHCLPSLKLVVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLID 121
           + LT A+L CLPSLKLV T+S GVDHLDLPELRRRGVAVA AGN+FS+DVAD+AVGLLID
Sbjct: 67  LALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAVGLLID 126

Query: 122 VLRKVSAADRFLRQGLWPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYT 181
           VLRKVSA DRF+RQGLW TK  FPLGLKLS +RIGI+GLG+IG EVAKRLEGFGCRISY 
Sbjct: 127 VLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGCRISYN 186

Query: 182 SRTKKPLVPYSYYSNVHELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRG 241
           SRTKKPLVPYSYYSNVHELAAECEALIICCGL  ET HMI+REVMVALGKDGVIIN+GRG
Sbjct: 187 SRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVIINIGRG 246

Query: 242 GIIDEKEMIECLIEGKIGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCE 301
            I+DEKEMI CL++G+I GAGLDVFENEPEIP+QL +L+NVVLSPH AV THE+F+ L +
Sbjct: 247 AIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETFLRLSK 306

Query: 302 LVVDNLEAFFSNKPLVS 318
           L+VDNLEAFFSN+PLVS
Sbjct: 307 LMVDNLEAFFSNRPLVS 322

[Close]

BLAST of MC05g_new0034.1 vs. NCBI nr
Match: XP_022159358.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Momordica charantia])

HSP 1 Score: 509 bits (1312), Expect = 9.37e-180
Identity = 255/319 (79.94%), Postives = 285/319 (89.34%), Query Frame = 0


Query: 4   QLPEVLALGSPLVFSLLESQFPNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGSIK 63
           +LP+VL LGSP V   LESQF +RFHFLKP  S+ PLLQFL+SYAQS QAL+I  GGS  
Sbjct: 9   ELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALLI-PGGSFA 68

Query: 64  LTPALLHCLPSLKLVVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLIDVL 123
           LT A+L CLPSLKL VT+SVGVDHLDLPELRRRG+AVA AGN++S+DVAD+AVGLLIDVL
Sbjct: 69  LTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAVGLLIDVL 128

Query: 124 RKVSAADRFLRQGLWPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYTSR 183
           RKVSAADRFLRQGLWPT  EFPLGLKLS +RIGI+GLGKIG EVAKRLEGFGC++SY SR
Sbjct: 129 RKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGCKMSYNSR 188

Query: 184 TKKPLVPYSYYSNVHELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRGGI 243
           TKKPLVPYSY+S VHELAAECEALI+CCGL  ET HMIDREVMVALGKDGVIINVGRG I
Sbjct: 189 TKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVIINVGRGAI 248

Query: 244 IDEKEMIECLIEGKIGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCELV 303
           IDEKE+IECL++G+IGGAGLDVFENEPEIP++L  ++NVVLSPH AV+THES  GLC+LV
Sbjct: 249 IDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESMKGLCKLV 308

Query: 304 VDNLEAFFSNKPLVSLFVD 322
           VDNLEAFFSNKPLVS F+D
Sbjct: 309 VDNLEAFFSNKPLVSPFMD 326

[Close]

BLAST of MC05g_new0034.1 vs. NCBI nr
Match: KAA0049013.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo var. makuwa] >TYK17551.1 glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo var. makuwa])

HSP 1 Score: 497 bits (1280), Expect = 5.22e-175
Identity = 247/319 (77.43%), Postives = 282/319 (88.40%), Query Frame = 0


Query: 4   QLPEVLALGSPLVFSLLESQFPNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGSIK 63
           +LP+VL LG P +F  LESQFPNRF FLKPWL  LPL QFLTSYAQSTQAL+IRGGGS +
Sbjct: 2   ELPQVLVLGPPSIFPYLESQFPNRFFFLKPWLYNLPLTQFLTSYAQSTQALLIRGGGSTQ 61

Query: 64  LTPALLHCLPSLKLVVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLIDVL 123
           LT A++ CLPSLKLVVTSSVGVDHLDLPELRRRGVA+ANAGN+FS+D AD+AVGLLIDVL
Sbjct: 62  LTSAIIDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIANAGNLFSEDAADMAVGLLIDVL 121

Query: 124 RKVSAADRFLRQGLWPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYTSR 183
           RKVSA DRF+RQGLW  KG+FP GLKLS +RIGI+GLGKIGSEVAKRLEGFGC++SY SR
Sbjct: 122 RKVSAGDRFVRQGLWSKKGDFPPGLKLSGKRIGILGLGKIGSEVAKRLEGFGCKVSYNSR 181

Query: 184 TKKPLVPYSYYSNVHELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRGGI 243
           TKK + PYSYYSNV+ELAA  EALIICC L  ET+H+I++EVM ALGKDGVI+NVGRG I
Sbjct: 182 TKKSMAPYSYYSNVYELAANTEALIICCALTKETYHLINKEVMQALGKDGVIVNVGRGLI 241

Query: 244 IDEKEMIECLIEGKIGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCELV 303
           IDEKEMI CLI+G+IGGAGLDVFENEP +P++L +L+NVVLSPH AV+T+ES V L +LV
Sbjct: 242 IDEKEMIRCLIQGEIGGAGLDVFENEPNVPEELFNLDNVVLSPHNAVMTYESKVELSKLV 301

Query: 304 VDNLEAFFSNKPLVSLFVD 322
           V+NLEAFFSNKPLVS  VD
Sbjct: 302 VNNLEAFFSNKPLVSPVVD 320

[Close]

BLAST of MC05g_new0034.1 vs. NCBI nr
Match: XP_023526607.1 (glyoxylate/hydroxypyruvate reductase HPR3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 496 bits (1276), Expect = 2.73e-174
Identity = 242/319 (75.86%), Postives = 281/319 (88.09%), Query Frame = 0


Query: 4   QLPEVLALGSPLVFSLLESQFPNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGSIK 63
           QLP+VL LG P VF  LESQFPNRFHFLKPWLS LPL QFL SYAQS  AL+IRGGG+++
Sbjct: 9   QLPQVLVLGPPPVFPYLESQFPNRFHFLKPWLSHLPLTQFLISYAQSATALLIRGGGTVQ 68

Query: 64  LTPALLHCLPSLKLVVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLIDVL 123
           LT A+L CLPSL+LVVT+SVGVDHLDLPELRRRG+A+A AGN+F++DVAD+A+GLLIDVL
Sbjct: 69  LTSAILDCLPSLQLVVTASVGVDHLDLPELRRRGLAIAYAGNVFTEDVADMAIGLLIDVL 128

Query: 124 RKVSAADRFLRQGLWPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYTSR 183
           RKVSA DRF+RQGLW TKG+FPLGLKLS +RIGI+GLGKIGSEVAKRLEGFGC++SY SR
Sbjct: 129 RKVSAGDRFVRQGLWSTKGDFPLGLKLSSKRIGIVGLGKIGSEVAKRLEGFGCKVSYYSR 188

Query: 184 TKKPLVPYSYYSNVHELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRGGI 243
            KK + PYSYYSNV+ELAA CE LIICC L  ETHH+I++EVMVALGKDGVI+N+GRG I
Sbjct: 189 IKKSMAPYSYYSNVYELAANCEVLIICCALTEETHHIINKEVMVALGKDGVIVNIGRGAI 248

Query: 244 IDEKEMIECLIEGKIGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCELV 303
           I+EKEMI CLIEG+IGGAGLDVFENEP IP++L  L+NVVL+PH AV T+ES + L +L+
Sbjct: 249 INEKEMIRCLIEGEIGGAGLDVFENEPRIPEELFHLDNVVLTPHVAVGTYESRMELSKLM 308

Query: 304 VDNLEAFFSNKPLVSLFVD 322
           V+NLE FFSNK LVS FVD
Sbjct: 309 VENLETFFSNKSLVSPFVD 327

[Close]

BLAST of MC05g_new0034.1 vs. NCBI nr
Match: XP_008438123.1 (PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo])

HSP 1 Score: 492 bits (1267), Expect = 4.98e-173
Identity = 245/319 (76.80%), Postives = 280/319 (87.77%), Query Frame = 0


Query: 4   QLPEVLALGSPLVFSLLESQFPNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGSIK 63
           +LP+VL LG P +F  LESQFPNRF FLKPWL  LPL QFLTSYAQSTQAL+IRGGGS +
Sbjct: 2   ELPQVLVLGPPSIFPYLESQFPNRFFFLKPWLYNLPLTQFLTSYAQSTQALLIRGGGSTQ 61

Query: 64  LTPALLHCLPSLKLVVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLIDVL 123
           LT A++ CLPSLKLVVTSSVGVDHLDLPELRRRGVA+ANAGN+FS+D AD+AVGLLIDVL
Sbjct: 62  LTSAIIDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIANAGNLFSEDAADMAVGLLIDVL 121

Query: 124 RKVSAADRFLRQGLWPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYTSR 183
           RKVSA DRF+RQGLW  KG+FP GLKLS +RIGI+GLGKIGSEVAKRLEGFGC++SY SR
Sbjct: 122 RKVSAGDRFVRQGLWSKKGDFPPGLKLSGKRIGIVGLGKIGSEVAKRLEGFGCKVSYNSR 181

Query: 184 TKKPLVPYSYYSNVHELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRGGI 243
           TKK + PYSYYSNV+ELAA  EALIICC L  ET+ +I++EVM ALGKDGVI+NVGRG I
Sbjct: 182 TKKSMAPYSYYSNVYELAANTEALIICCALTKETYRLINKEVMQALGKDGVIVNVGRGLI 241

Query: 244 IDEKEMIECLIEGKIGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCELV 303
           IDEKEMI CLI+G+IGGAGLDVFENEP +P++L +L+NVVLSPH AV+T+ES V L +LV
Sbjct: 242 IDEKEMIRCLIQGEIGGAGLDVFENEPNVPEELFNLDNVVLSPHNAVMTYESKVELSKLV 301

Query: 304 VDNLEAFFSNKPLVSLFVD 322
           V+NLEAFFSN PLVS  VD
Sbjct: 302 VNNLEAFFSNTPLVSPVVD 320

[Close]

BLAST of MC05g_new0034.1 vs. ExPASy TrEMBL
Match: A0A6J1D1V9 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN=LOC111016253 PE=3 SV=1)

HSP 1 Score: 513 bits (1321), Expect = 2.32e-181
Identity = 257/317 (81.07%), Postives = 282/317 (88.96%), Query Frame = 0


Query: 2   DPQLPEVLALGSPLVFSLLESQFPNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGS 61
           D  LP+VL LG P VFS LESQFPNRFHFLKPWLSKLPL QFLTSYAQ  QALII  GGS
Sbjct: 7   DQDLPQVLVLGPPTVFSCLESQFPNRFHFLKPWLSKLPLPQFLTSYAQYVQALII-PGGS 66

Query: 62  IKLTPALLHCLPSLKLVVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLID 121
           + LT A+L CLPSLKLV T+S GVDHLDLPELRRRGVAVA AGN+FS+DVAD+AVGLLID
Sbjct: 67  LALTSAILDCLPSLKLVATASAGVDHLDLPELRRRGVAVAYAGNLFSEDVADMAVGLLID 126

Query: 122 VLRKVSAADRFLRQGLWPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYT 181
           VLRKVSA DRF+RQGLW TK  FPLGLKLS +RIGI+GLG+IG EVAKRLEGFGCRISY 
Sbjct: 127 VLRKVSAGDRFMRQGLWSTKVNFPLGLKLSGKRIGIVGLGRIGCEVAKRLEGFGCRISYN 186

Query: 182 SRTKKPLVPYSYYSNVHELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRG 241
           SRTKKPLVPYSYYSNVHELAAECEALIICCGL  ET HMI+REVMVALGKDGVIIN+GRG
Sbjct: 187 SRTKKPLVPYSYYSNVHELAAECEALIICCGLTEETRHMINREVMVALGKDGVIINIGRG 246

Query: 242 GIIDEKEMIECLIEGKIGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCE 301
            I+DEKEMI CL++G+I GAGLDVFENEPEIP+QL +L+NVVLSPH AV THE+F+ L +
Sbjct: 247 AIVDEKEMIRCLLQGEIWGAGLDVFENEPEIPEQLFTLDNVVLSPHVAVTTHETFLRLSK 306

Query: 302 LVVDNLEAFFSNKPLVS 318
           L+VDNLEAFFSN+PLVS
Sbjct: 307 LMVDNLEAFFSNRPLVS 322

[Close]

BLAST of MC05g_new0034.1 vs. ExPASy TrEMBL
Match: A0A6J1DYK8 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN=LOC111025775 PE=3 SV=1)

HSP 1 Score: 509 bits (1312), Expect = 4.54e-180
Identity = 255/319 (79.94%), Postives = 285/319 (89.34%), Query Frame = 0


Query: 4   QLPEVLALGSPLVFSLLESQFPNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGSIK 63
           +LP+VL LGSP V   LESQF +RFHFLKP  S+ PLLQFL+SYAQS QAL+I  GGS  
Sbjct: 9   ELPQVLVLGSPWVLPSLESQFSHRFHFLKPSNSQFPLLQFLSSYAQSVQALLI-PGGSFA 68

Query: 64  LTPALLHCLPSLKLVVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLIDVL 123
           LT A+L CLPSLKL VT+SVGVDHLDLPELRRRG+AVA AGN++S+DVAD+AVGLLIDVL
Sbjct: 69  LTSAILDCLPSLKLAVTTSVGVDHLDLPELRRRGIAVAYAGNLYSEDVADMAVGLLIDVL 128

Query: 124 RKVSAADRFLRQGLWPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYTSR 183
           RKVSAADRFLRQGLWPT  EFPLGLKLS +RIGI+GLGKIG EVAKRLEGFGC++SY SR
Sbjct: 129 RKVSAADRFLRQGLWPTNREFPLGLKLSGKRIGIVGLGKIGCEVAKRLEGFGCKMSYNSR 188

Query: 184 TKKPLVPYSYYSNVHELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRGGI 243
           TKKPLVPYSY+S VHELAAECEALI+CCGL  ET HMIDREVMVALGKDGVIINVGRG I
Sbjct: 189 TKKPLVPYSYHSTVHELAAECEALIVCCGLTEETRHMIDREVMVALGKDGVIINVGRGAI 248

Query: 244 IDEKEMIECLIEGKIGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCELV 303
           IDEKE+IECL++G+IGGAGLDVFENEPEIP++L  ++NVVLSPH AV+THES  GLC+LV
Sbjct: 249 IDEKELIECLLQGEIGGAGLDVFENEPEIPEELFGVDNVVLSPHVAVITHESMKGLCKLV 308

Query: 304 VDNLEAFFSNKPLVSLFVD 322
           VDNLEAFFSNKPLVS F+D
Sbjct: 309 VDNLEAFFSNKPLVSPFMD 326

[Close]

BLAST of MC05g_new0034.1 vs. ExPASy TrEMBL
Match: A0A5A7TZG5 (Glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004210 PE=3 SV=1)

HSP 1 Score: 497 bits (1280), Expect = 2.53e-175
Identity = 247/319 (77.43%), Postives = 282/319 (88.40%), Query Frame = 0


Query: 4   QLPEVLALGSPLVFSLLESQFPNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGSIK 63
           +LP+VL LG P +F  LESQFPNRF FLKPWL  LPL QFLTSYAQSTQAL+IRGGGS +
Sbjct: 2   ELPQVLVLGPPSIFPYLESQFPNRFFFLKPWLYNLPLTQFLTSYAQSTQALLIRGGGSTQ 61

Query: 64  LTPALLHCLPSLKLVVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLIDVL 123
           LT A++ CLPSLKLVVTSSVGVDHLDLPELRRRGVA+ANAGN+FS+D AD+AVGLLIDVL
Sbjct: 62  LTSAIIDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIANAGNLFSEDAADMAVGLLIDVL 121

Query: 124 RKVSAADRFLRQGLWPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYTSR 183
           RKVSA DRF+RQGLW  KG+FP GLKLS +RIGI+GLGKIGSEVAKRLEGFGC++SY SR
Sbjct: 122 RKVSAGDRFVRQGLWSKKGDFPPGLKLSGKRIGILGLGKIGSEVAKRLEGFGCKVSYNSR 181

Query: 184 TKKPLVPYSYYSNVHELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRGGI 243
           TKK + PYSYYSNV+ELAA  EALIICC L  ET+H+I++EVM ALGKDGVI+NVGRG I
Sbjct: 182 TKKSMAPYSYYSNVYELAANTEALIICCALTKETYHLINKEVMQALGKDGVIVNVGRGLI 241

Query: 244 IDEKEMIECLIEGKIGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCELV 303
           IDEKEMI CLI+G+IGGAGLDVFENEP +P++L +L+NVVLSPH AV+T+ES V L +LV
Sbjct: 242 IDEKEMIRCLIQGEIGGAGLDVFENEPNVPEELFNLDNVVLSPHNAVMTYESKVELSKLV 301

Query: 304 VDNLEAFFSNKPLVSLFVD 322
           V+NLEAFFSNKPLVS  VD
Sbjct: 302 VNNLEAFFSNKPLVSPVVD 320

[Close]

BLAST of MC05g_new0034.1 vs. ExPASy TrEMBL
Match: A0A1S3AW81 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo OX=3656 GN=LOC103483325 PE=3 SV=1)

HSP 1 Score: 492 bits (1267), Expect = 2.41e-173
Identity = 245/319 (76.80%), Postives = 280/319 (87.77%), Query Frame = 0


Query: 4   QLPEVLALGSPLVFSLLESQFPNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGSIK 63
           +LP+VL LG P +F  LESQFPNRF FLKPWL  LPL QFLTSYAQSTQAL+IRGGGS +
Sbjct: 2   ELPQVLVLGPPSIFPYLESQFPNRFFFLKPWLYNLPLTQFLTSYAQSTQALLIRGGGSTQ 61

Query: 64  LTPALLHCLPSLKLVVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLIDVL 123
           LT A++ CLPSLKLVVTSSVGVDHLDLPELRRRGVA+ANAGN+FS+D AD+AVGLLIDVL
Sbjct: 62  LTSAIIDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIANAGNLFSEDAADMAVGLLIDVL 121

Query: 124 RKVSAADRFLRQGLWPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYTSR 183
           RKVSA DRF+RQGLW  KG+FP GLKLS +RIGI+GLGKIGSEVAKRLEGFGC++SY SR
Sbjct: 122 RKVSAGDRFVRQGLWSKKGDFPPGLKLSGKRIGIVGLGKIGSEVAKRLEGFGCKVSYNSR 181

Query: 184 TKKPLVPYSYYSNVHELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRGGI 243
           TKK + PYSYYSNV+ELAA  EALIICC L  ET+ +I++EVM ALGKDGVI+NVGRG I
Sbjct: 182 TKKSMAPYSYYSNVYELAANTEALIICCALTKETYRLINKEVMQALGKDGVIVNVGRGLI 241

Query: 244 IDEKEMIECLIEGKIGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCELV 303
           IDEKEMI CLI+G+IGGAGLDVFENEP +P++L +L+NVVLSPH AV+T+ES V L +LV
Sbjct: 242 IDEKEMIRCLIQGEIGGAGLDVFENEPNVPEELFNLDNVVLSPHNAVMTYESKVELSKLV 301

Query: 304 VDNLEAFFSNKPLVSLFVD 322
           V+NLEAFFSN PLVS  VD
Sbjct: 302 VNNLEAFFSNTPLVSPVVD 320

[Close]

BLAST of MC05g_new0034.1 vs. ExPASy TrEMBL
Match: A0A6J1EEZ0 (glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucurbita moschata OX=3662 GN=LOC111431925 PE=3 SV=1)

HSP 1 Score: 490 bits (1261), Expect = 2.54e-172
Identity = 241/319 (75.55%), Postives = 280/319 (87.77%), Query Frame = 0


Query: 4   QLPEVLALGSPLVFSLLESQFPNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGSIK 63
           +LP+VL LG P VF  LESQFPNRFHFLKPWLS LPL QFL SYAQS  AL+IRGGG+++
Sbjct: 9   ELPQVLVLGPPPVFPYLESQFPNRFHFLKPWLSNLPLTQFLISYAQSATALLIRGGGTVQ 68

Query: 64  LTPALLHCLPSLKLVVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLIDVL 123
           LT A+L CLPSL+LVVT+SVGVDHLDLPELRRRG+A+A AGN+FS+DVAD+A+GLLIDV+
Sbjct: 69  LTSAILDCLPSLQLVVTASVGVDHLDLPELRRRGLAIAYAGNVFSEDVADMAIGLLIDVI 128

Query: 124 RKVSAADRFLRQGLWPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYTSR 183
           RKVSA DRF+RQGL  TKG+FPLGLKLS +RIGI+GLG+IGSEVAKRLEGFGC+ISY SR
Sbjct: 129 RKVSAGDRFVRQGLRSTKGDFPLGLKLSSKRIGIVGLGQIGSEVAKRLEGFGCKISYYSR 188

Query: 184 TKKPLVPYSYYSNVHELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRGGI 243
            KK + PYSYYSNV+ELAA CE LIICC L  ETHH+I+REVMVALGKDGVI+N+GRG I
Sbjct: 189 IKKSMAPYSYYSNVYELAANCEVLIICCALTEETHHIINREVMVALGKDGVIVNIGRGAI 248

Query: 244 IDEKEMIECLIEGKIGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCELV 303
           I+EKEMI CLIEG+IGGAGLDVFENEP IP++L  L+NVVL+PH AV T+ES + L +L+
Sbjct: 249 INEKEMIRCLIEGEIGGAGLDVFENEPRIPEELFHLDNVVLTPHVAVGTYESRMELSKLM 308

Query: 304 VDNLEAFFSNKPLVSLFVD 322
           V+NLE FFSNK LVS FVD
Sbjct: 309 VENLETFFSNKSLVSPFVD 327

[Close]

BLAST of MC05g_new0034.1 vs. TAIR 10
Match: AT2G45630.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 347.4 bits (890), Expect = 1.2e-95
Identity = 176/318 (55.35%), Postives = 237/318 (74.53%), Query Frame = 0


Query: 4   QLPEVLALGSPLVFSLLESQF--PNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGS 63
           +LP VL +  P   ++L   F    +F  LK + S LPL +FL  ++ S  A+I      
Sbjct: 20  KLPRVLIVKRPDAMAVLGDGFVASTKFEILKAFESPLPLPEFLAYHSDSISAIIAPVAAP 79

Query: 64  IKLTPALLHCLPSLKLVVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLID 123
           +  T  L+  LP+L+LVVT+S GVDH+DL E RRRG++VANAG+ FS+DVAD AVGLLID
Sbjct: 80  V--TADLIRILPNLRLVVTTSAGVDHVDLVECRRRGISVANAGSSFSEDVADTAVGLLID 139

Query: 124 VLRKVSAADRFLRQGLWPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYT 183
           V R++SAA+RF++Q  WP KG++PLG KL ++RIGI+GLG IGS+VA RL+ FGC+ISY+
Sbjct: 140 VFRRISAANRFVKQRFWPLKGDYPLGSKLGRKRIGIVGLGSIGSKVATRLDAFGCQISYS 199

Query: 184 SRTKKPL-VPYSYYSNVHELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGR 243
           SR +KP  VPY YY ++ E+AA  +ALIICC LN +T  +I+++V+ ALGK GVI+NV R
Sbjct: 200 SRNRKPYDVPYHYYMDIEEMAANSDALIICCELNEKTLRLINKDVLSALGKRGVIVNVAR 259

Query: 244 GGIIDEKEMIECLIEGKIGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLC 303
           G IIDE+EM+ CL EG+IGGAGLDVFE+EP +P++L  L+NVV SPH+A +T E    L 
Sbjct: 260 GAIIDEEEMVRCLREGEIGGAGLDVFEDEPNVPKELFELDNVVFSPHSAFMTLEGLEELG 319

Query: 304 ELVVDNLEAFFSNKPLVS 319
           ++VV N+EAFFSNKPL++
Sbjct: 320 KVVVGNIEAFFSNKPLLT 335

[Close]

BLAST of MC05g_new0034.1 vs. TAIR 10
Match: AT1G12550.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 332.0 bits (850), Expect = 5.2e-91
Identity = 166/314 (52.87%), Postives = 220/314 (70.06%), Query Frame = 0


Query: 6   PEVLALGSPLVFSLLESQFPNRFH-FLKPWLSKLPLLQFLTSYAQSTQALIIRGGGSIKL 65
           P V+ L  P   + ++      F   +    S   L  F   +A S +A +I   G + +
Sbjct: 7   PPVVLLHRPPSLTFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVI--SGRLPV 66

Query: 66  TPALLHCLPSLKLVVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLIDVLR 125
           T  LL  LPSL+++V +SVG+DH+DL   +RRG+ + NAGN FSDDVAD AVGLLI VLR
Sbjct: 67  TDELLSHLPSLQILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLLISVLR 126

Query: 126 KVSAADRFLRQGLWPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYTSRT 185
           ++ AADR++R G W   G+F LG K+S +R+GI+GLG IGS VAKRLE FGC ISY SR+
Sbjct: 127 RIPAADRYVRSGNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVISYNSRS 186

Query: 186 KKPLVPYSYYSNVHELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRGGII 245
           +K   PY YYS++  LA   + L++CC L  ETHH+++REVM  LGKDGV+INVGRG +I
Sbjct: 187 QKQSSPYRYYSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVGRGKLI 246

Query: 246 DEKEMIECLIEGKIGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCELVV 305
           DEKEM++CL++G IGGAGLDVFENEP +PQ+L  L+NVVLSPH AV T  S   + ++ +
Sbjct: 247 DEKEMVKCLVDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNVAQIAL 306

Query: 306 DNLEAFFSNKPLVS 319
            NL+AFFSN+PL+S
Sbjct: 307 ANLKAFFSNRPLLS 318

[Close]

BLAST of MC05g_new0034.1 vs. TAIR 10
Match: AT1G79870.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 263.1 bits (671), Expect = 3.0e-70
Identity = 135/301 (44.85%), Postives = 199/301 (66.11%), Query Frame = 0


Query: 18  SLLESQFPNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGSIKLTPALLHCLPSLKL 77
           S LE++   RF+ L+ W S    +  L ++  S +A++  G  S      L+  LP+L++
Sbjct: 14  SYLENELEKRFNLLRFWTSPEKSV-LLETHRNSIRAVV--GNASAGADAQLISDLPNLEI 73

Query: 78  VVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLIDVLRKVSAADRFLRQGL 137
           V + SVG+D +DL + + +G+ V N  ++ ++DVADLA+GL++ +LR++   DR++R G 
Sbjct: 74  VSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLCECDRYVRSGK 133

Query: 138 WPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYTSRTKKPLVPYSYYSNV 197
           W  +GEF L  K S + +GIIGLG+IG+ +AKR E F C I+Y SRT KP V Y YY  V
Sbjct: 134 W-KQGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIKPDVAYKYYPTV 193

Query: 198 HELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRGGIIDEKEMIECLIEGK 257
            +LA   + L++ C L  +T H++DR+VM ALG  GV+IN+GRG  +DE+E+I+ L EG+
Sbjct: 194 VDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQELIKALTEGR 253

Query: 258 IGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCELVVDNLEAFFSNKPLV 317
           +GGA LDVFE EP +P++L  LENVVL PH    T E+   + +LVV NLEA FS K L+
Sbjct: 254 LGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNLEAHFSGKSLL 310

Query: 318 S 319
           +
Sbjct: 314 T 310

[Close]

BLAST of MC05g_new0034.1 vs. TAIR 10
Match: AT1G79870.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 234.6 bits (597), Expect = 1.1e-61
Identity = 124/301 (41.20%), Postives = 185/301 (61.46%), Query Frame = 0


Query: 18  SLLESQFPNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGSIKLTPALLHCLPSLKL 77
           S LE++   RF+ L+ W S    +  L ++  S +A++  G  S      L+  LP+L++
Sbjct: 14  SYLENELEKRFNLLRFWTSPEKSV-LLETHRNSIRAVV--GNASAGADAQLISDLPNLEI 73

Query: 78  VVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLIDVLRKVSAADRFLRQGL 137
           V + SVG+D +DL + + +G+ V N  ++ ++DVADLA+GL++ +LR++   DR++R G 
Sbjct: 74  VSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLCECDRYVRSGK 133

Query: 138 WPTKGEFPLGLKLSQRRIGIIGLGKIGSEVAKRLEGFGCRISYTSRTKKPLVPYSYYSNV 197
           W                      G+IG+ +AKR E F C I+Y SRT KP V Y YY  V
Sbjct: 134 WKQ--------------------GRIGTAIAKRAEAFSCPINYYSRTIKPDVAYKYYPTV 193

Query: 198 HELAAECEALIICCGLNGETHHMIDREVMVALGKDGVIINVGRGGIIDEKEMIECLIEGK 257
            +LA   + L++ C L  +T H++DR+VM ALG  GV+IN+GRG  +DE+E+I+ L EG+
Sbjct: 194 VDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQELIKALTEGR 253

Query: 258 IGGAGLDVFENEPEIPQQLCSLENVVLSPHAAVVTHESFVGLCELVVDNLEAFFSNKPLV 317
           +GGA LDVFE EP +P++L  LENVVL PH    T E+   + +LVV NLEA FS K L+
Sbjct: 254 LGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNLEAHFSGKSLL 291

Query: 318 S 319
           +
Sbjct: 314 T 291

[Close]

BLAST of MC05g_new0034.1 vs. TAIR 10
Match: AT2G45630.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 138.7 bits (348), Expect = 8.5e-33
Identity = 74/149 (49.66%), Postives = 102/149 (68.46%), Query Frame = 0


Query: 4   QLPEVLALGSPLVFSLLESQF--PNRFHFLKPWLSKLPLLQFLTSYAQSTQALIIRGGGS 63
           +LP VL +  P   ++L   F    +F  LK + S LPL +FL  ++ S  A+I      
Sbjct: 20  KLPRVLIVKRPDAMAVLGDGFVASTKFEILKAFESPLPLPEFLAYHSDSISAIIAPVAAP 79

Query: 64  IKLTPALLHCLPSLKLVVTSSVGVDHLDLPELRRRGVAVANAGNIFSDDVADLAVGLLID 123
           +  T  L+  LP+L+LVVT+S GVDH+DL E RRRG++VANAG+ FS+DVAD AVGLLID
Sbjct: 80  V--TADLIRILPNLRLVVTTSAGVDHVDLVECRRRGISVANAGSSFSEDVADTAVGLLID 139

Query: 124 VLRKVSAADRFLRQGLWPTKGEFPLGLKL 151
           V R++SAA+RF++Q  WP KG++PLG K+
Sbjct: 140 VFRRISAANRFVKQRFWPLKGDYPLGSKV 166

The following BLAST results are available for this feature:

BLAST of MC05g_new0034.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LE33	7.4e-90	52.87	Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR...	[more]
Q65CJ7	3.4e-71	46.49	Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR ...	[more]
A5CAL1	2.2e-70	44.33	Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN...	[more]
Q9CA90	4.2e-69	44.85	Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=H...	[more]
Q5FTU6	3.6e-52	36.19	2-ketogluconate reductase OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=GO...	[more]

BLAST of MC05g_new0034.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147257.1	4.79e-181	81.07	glyoxylate/hydroxypyruvate reductase HPR3-like [Momordica charantia]	[more]
XP_022159358.1	9.37e-180	79.94	glyoxylate/hydroxypyruvate reductase HPR3-like [Momordica charantia]	[more]
KAA0049013.1	5.22e-175	77.43	glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo var. makuwa] >TYK17...	[more]
XP_023526607.1	2.73e-174	75.86	glyoxylate/hydroxypyruvate reductase HPR3-like [Cucurbita pepo subsp. pepo]	[more]
XP_008438123.1	4.98e-173	76.80	PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo]	[more]

BLAST of MC05g_new0034.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D1V9	2.32e-181	81.07	glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1DYK8	4.54e-180	79.94	glyoxylate/hydroxypyruvate reductase HPR3-like OS=Momordica charantia OX=3673 GN...	[more]
A0A5A7TZG5	2.53e-175	77.43	Glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3AW81	2.41e-173	76.80	glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A6J1EEZ0	2.54e-172	75.55	glyoxylate/hydroxypyruvate reductase HPR3-like OS=Cucurbita moschata OX=3662 GN=...	[more]

BLAST of MC05g_new0034.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45630.2	1.2e-95	55.35	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]
AT1G12550.1	5.2e-91	52.87	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]
AT1G79870.1	3.0e-70	44.85	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]
AT1G79870.2	1.1e-61	41.20	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]
AT2G45630.1	8.5e-33	49.66	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006139	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain	PFAM	PF00389	2-Hacid_dh	coord: 48..316 e-value: 3.7E-17 score: 62.6
None	No IPR available	GENE3D	3.40.50.720		coord: 24..315 e-value: 6.2E-101 score: 339.0
None	No IPR available	GENE3D	3.40.50.720		coord: 108..292 e-value: 6.2E-101 score: 339.0
None	No IPR available	PANTHER	PTHR10996:SF179	D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN-RELATED	coord: 2..318
None	No IPR available	PANTHER	PTHR10996	2-HYDROXYACID DEHYDROGENASE-RELATED	coord: 2..318
None	No IPR available	CDD	cd12156	HPPR	coord: 6..312 e-value: 8.61818E-128 score: 365.253
None	No IPR available	SUPERFAMILY	52283	Formate/glycerate dehydrogenase catalytic domain-like	coord: 39..142
IPR006140	D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain	PFAM	PF02826	2-Hacid_dh_C	coord: 116..289 e-value: 1.0E-48 score: 165.0
IPR029752	D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved site 1	PROSITE	PS00065	D_2_HYDROXYACID_DH_1	coord: 155..182
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 108..289

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC05g_new0034	MC05g_new0034	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC05g_new0034.1-cds	MC05g_new0034.1-cds-MC05:2325607..2326053	CDS
MC05g_new0034.1-cds	MC05g_new0034.1-cds-MC05:2326337..2326858	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC05g_new0034.1	MC05g_new0034.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005829	cytosol
molecular_function	GO:0051287	NAD binding
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
molecular_function	GO:0030267	glyoxylate reductase (NADP+) activity
molecular_function	GO:0016618	hydroxypyruvate reductase activity