MC05g1568.1 (mRNA) Bitter gourd (Dali-11) v1

BLAST of MC05g1568.1 vs. ExPASy Swiss-Prot
Match: Q9SR96 (DnaJ protein ERDJ3A OS=Arabidopsis thaliana OX=3702 GN=ERDJ3A PE=1 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 1.9e-185
Identity = 342/576 (59.38%), Postives = 437/576 (75.87%), Query Frame = 0

Query: 96  METRLAPIIFIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 155
           + TRLA  + ++++  L LL+++AK++DPYKVLGV ++A QREIQKAFHK SL+YHPDKN
Sbjct: 2   VRTRLAISVVLVST--LLLLNVKAKSVDPYKVLGVSKDAKQREIQKAFHKQSLKYHPDKN 61

Query: 156 KQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGG- 215
           K KGAQ+KF EINNAYEILSDEEKRKN+D+YGDEKG PGF  G PG   GGY++ ++GG 
Sbjct: 62  KDKGAQEKFAEINNAYEILSDEEKRKNYDLYGDEKGQPGFDSGFPGG-NGGYSYSSSGGG 121

Query: 216 ---QRQGQWQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGGG----NFGGF 275
                 G WQ+ GGG G S+SFSFSFGGPS S SFGFGM+DIFS F GG       FGGF
Sbjct: 122 FNFGGPGGWQNMGGG-GGSKSFSFSFGGPSES-SFGFGMDDIFSMFSGGSSKGKEQFGGF 181

Query: 276 GGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEVA 335
           G +S A++ S++ +  + + IN QV++K++VDQGM WLL     S +G  + ESIIEEVA
Sbjct: 182 GSSSNAESKSKSSTVAAIKTINSQVYKKDVVDQGMTWLLLSYLPSQRGSQYHESIIEEVA 241

Query: 336 SSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNLK 395
            SLQGALKVGR++CE ESSLCK LGI P R PR+FVYSY  S + +L EY+ ++  K +K
Sbjct: 242 ESLQGALKVGRLNCETESSLCKQLGIVPRRAPRMFVYSYTSSGKATLAEYTEELVAKKVK 301

Query: 396 SFCQEHLPRFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFYD 455
           SFCQEHLPRFS+++DL  FD S  +    P ++LLSTKKDTPVIWRVLSGLY  +F FY+
Sbjct: 302 SFCQEHLPRFSKKIDLNTFDVSAVSSQKTPKVLLLSTKKDTPVIWRVLSGLYNGRFVFYN 361

Query: 456 AEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEKK 515
            EV+D SDP ++KLGVD  PAIVGWLSNGE+ +L+ GI VK+L+SA+ +L  L  G EKK
Sbjct: 362 TEVHDTSDPKIQKLGVDKFPAIVGWLSNGEKQVLKTGITVKNLKSAVQELGKLLEGLEKK 421

Query: 516 NKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKARNKLESILSM 575
           NKK +S+  +    + +  +IPLL+  +FD+ICGE TPVC+IGAF+SS  + KL+SI+S 
Sbjct: 422 NKKVSSKSQAGQAPNESSEKIPLLSRPNFDSICGENTPVCIIGAFRSSNGKEKLQSIMSK 481

Query: 576 VSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNGEFK-KSDKVLVAYKRRKGKF 635
           VSQK+LSRRQ S+ GS++++SY+L+DATKQ +FL + D  EFK  SDK+L+AYK R+GKF
Sbjct: 482 VSQKSLSRRQASTTGSQDTVSYSLLDATKQSAFLSSLDKSEFKTSSDKLLIAYKPRRGKF 541

Query: 636 AAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 663
           A   G+MT EEVEKF+ +V NGDI FTKTRQKP  K
Sbjct: 542 ATFKGDMTIEEVEKFVAAVLNGDIQFTKTRQKPQIK 572

BLAST of MC05g1568.1 vs. ExPASy Swiss-Prot
Match: Q10MW6 (DnaJ protein ERDJ3A OS=Oryza sativa subsp. japonica OX=39947 GN=ERDJ3A PE=1 SV=2)

HSP 1 Score: 555.1 bits (1429), Expect = 1.1e-156
Identity = 300/577 (51.99%), Postives = 403/577 (69.84%), Query Frame = 0

Query: 106 IMASLFLFLLSLE---AKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKQKGAQQ 165
           ++AS+ L  ++L    AK +DPYKVLGV+++ASQR+IQKAFHKLSL+YHPDKNK KGAQ+
Sbjct: 9   LVASIVLSSIALHVAAAKNLDPYKVLGVDKSASQRDIQKAFHKLSLKYHPDKNKSKGAQE 68

Query: 166 KFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQRQGQWQS 225
           KF EINNAY+ILSDEEKRKN+D+YGDEKG+PGFG G+ G++  GYT+FT GG +   + S
Sbjct: 69  KFAEINNAYDILSDEEKRKNYDLYGDEKGNPGFGGGNFGNR-EGYTYFTGGGAKTSHFSS 128

Query: 226 -----TGGGQGASQSFSFSFG----GPSGSNSFGFGMEDIFSNFFGGGGNFGG--FGGNS 285
                T GGQG +++FSFSFG    G  G N F F   D+FSN F GG   G    G   
Sbjct: 129 GDGWQTMGGQGNTKTFSFSFGGGNPGAGGGNPFNFDFGDVFSNIFSGGSMGGSQHTGSAG 188

Query: 286 KAQTGSRT----RSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEVA 345
           KA+ G+++     S+ + + +  Q+F KE  DQG+ WLL   T + KG   +ES++E+VA
Sbjct: 189 KARRGTKSSGHDSSSVNIQEVTMQIFNKETADQGITWLLLFYTPNTKGQFVLESVVEDVA 248

Query: 346 SSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNLK 405
            SL GAL+ G+++C+ E +LCK  G+   ++ R+F+YSY  + +GSL EYSGD   K+LK
Sbjct: 249 RSLDGALRAGKVNCDHEKALCKKAGVSIGKSARLFIYSYTTTEKGSLHEYSGDYDSKSLK 308

Query: 406 SFCQEHLPRFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFYD 465
           +FCQEHLPRFS+RVD+ QF F  +    LP ++LLS KKDTP +WR +SG++R +  FYD
Sbjct: 309 TFCQEHLPRFSKRVDINQFSFPSNIIPNLPQVLLLSAKKDTPAMWRAVSGMFRSRLIFYD 368

Query: 466 AEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEKK 525
           AEV DVS P +K LGV  +PA++G   NGE  +L+ GI+VKDLRS I +L NL   FEKK
Sbjct: 369 AEVQDVSHPLLKSLGVKNIPALIGRSVNGEEQLLKDGISVKDLRSGIKELKNLLENFEKK 428

Query: 526 NKKTASRPTSKT--PSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKARNKLESIL 585
           NKK AS    K        EN+IPLLT S+F+ ICGEKT VC++G FKSSKA+  LE++L
Sbjct: 429 NKKLASNQAKKPAHTDQPKENKIPLLTASNFEEICGEKTSVCILGIFKSSKAKENLEAVL 488

Query: 586 SMVSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNGEFKKSDKVLVAYKRRKGK 645
           S +SQKTL R QN + G+  +++YAL+D  KQ +FL  FD   FK SDK+L+AYK R+G+
Sbjct: 489 SEISQKTLIRGQNYNSGN--AVAYALLDGNKQSAFLSTFDKSAFKSSDKLLLAYKPRRGR 548

Query: 646 FAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 663
           +A +  E+T EE E+F+ SV NGD+  +   +KPV +
Sbjct: 549 YAVYDNEVTMEEAERFVVSVLNGDVQLSAAGRKPVLR 582

BLAST of MC05g1568.1 vs. ExPASy Swiss-Prot
Match: Q8EUM4 (Chaperone protein DnaJ OS=Mycoplasma penetrans (strain HF-2) OX=272633 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 2.2e-19
Identity = 62/139 (44.60%), Postives = 82/139 (58.99%), Query Frame = 0

Query: 123 DPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKN 182
           D Y+VLGV ++A+  +I+ AF K ++QYHPD+NK+  A++KF+E+N AYE+LSD +KR N
Sbjct: 6   DYYEVLGVSKDATDDQIKSAFRKKAMQYHPDRNKEPDAEEKFKEVNQAYEVLSDPDKRAN 65

Query: 183 FDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQRQGQWQSTGGGQGASQSFSFSFGGPSG 242
           +D +G E     FG G   D    +  F          Q  GGG+G    F  SFGG   
Sbjct: 66  YDRFGHEGVDGQFGGGAGFDP---FDIFN---------QFFGGGRGGGAHFEQSFGGS-- 124

Query: 243 SNSFGFGMEDIFSNFFGGG 262
                 G EDIFSNFFGGG
Sbjct: 126 ------GFEDIFSNFFGGG 124

BLAST of MC05g1568.1 vs. ExPASy Swiss-Prot
Match: P35514 (Chaperone protein DnaJ OS=Lactococcus lactis subsp. lactis (strain IL1403) OX=272623 GN=dnaJ PE=3 SV=2)

HSP 1 Score: 98.2 bits (243), Expect = 3.7e-19
Identity = 64/138 (46.38%), Postives = 84/138 (60.87%), Query Frame = 0

Query: 125 YKVLGVERNASQREIQKAFHKLSLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFD 184
           Y+ LGV++NASQ EI+KA+ K+S +YHPD NK++GA++K++E+  AYE LSDE+KR  +D
Sbjct: 7   YERLGVDKNASQDEIKKAYRKMSKKYHPDLNKEEGAEEKYKEVQEAYETLSDEQKRAAYD 66

Query: 185 MYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQRQGQWQSTGGGQGASQSFSFSFGGPSGSN 244
            YG+   + GFG                           GGG G +  FS  FGG  GS 
Sbjct: 67  QYGEAGANGGFG---------------------------GGGFGGASGFS-GFGG--GSG 113

Query: 245 SFGFGMEDIFSNFFGGGG 263
            FG G EDIFS+FFGGGG
Sbjct: 127 GFG-GFEDIFSSFFGGGG 113

BLAST of MC05g1568.1 vs. ExPASy Swiss-Prot
Match: A2RP20 (Chaperone protein DnaJ OS=Lactococcus lactis subsp. cremoris (strain MG1363) OX=416870 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 6.3e-19
Identity = 64/138 (46.38%), Postives = 83/138 (60.14%), Query Frame = 0

Query: 125 YKVLGVERNASQREIQKAFHKLSLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKNFD 184
           Y+ LGV++NASQ EI+KA+ K+S +YHPD NK++GA+ K++E+  AYE LSDE+KR  +D
Sbjct: 7   YERLGVDKNASQDEIKKAYRKMSKKYHPDLNKEEGAEDKYKEVQEAYETLSDEQKRAAYD 66

Query: 185 MYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQRQGQWQSTGGGQGASQSFSFSFGGPSGSN 244
            YG+   + GFG                           GGG G +  FS  FGG SG  
Sbjct: 67  QYGEAGANGGFG---------------------------GGGFGGASGFS-GFGGASG-- 113

Query: 245 SFGFGMEDIFSNFFGGGG 263
            FG G EDIFS+FFGGGG
Sbjct: 127 GFG-GFEDIFSSFFGGGG 113

BLAST of MC05g1568.1 vs. NCBI nr
Match: XP_022145676.1 (dnaJ protein ERDJ3A [Momordica charantia])

HSP 1 Score: 1093 bits (2827), Expect = 0.0
Identity = 566/567 (99.82%), Postives = 566/567 (99.82%), Query Frame = 0

Query: 96  METRLAPIIFIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 155
           METRLAPIIFIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   METRLAPIIFIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 156 KQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQ 215
           KQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQ
Sbjct: 61  KQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQ 120

Query: 216 RQGQWQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGGGNFGGFGGNSKAQT 275
           RQGQWQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGGGNFGGFGGNSKAQT
Sbjct: 121 RQGQWQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGGGNFGGFGGNSKAQT 180

Query: 276 GSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEVASSLQGALK 335
           GSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEVASSLQGALK
Sbjct: 181 GSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEVASSLQGALK 240

Query: 336 VGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNLKSFCQEHLP 395
           VGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNLKSFCQEHLP
Sbjct: 241 VGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNLKSFCQEHLP 300

Query: 396 RFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFYDAEVNDVSD 455
           RFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFYDAEVNDVSD
Sbjct: 301 RFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFYDAEVNDVSD 360

Query: 456 PTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEKKNKKTASRP 515
           PTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEKKNKKTASRP
Sbjct: 361 PTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEKKNKKTASRP 420

Query: 516 TSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKARNKLESILSMVSQKTLSR 575
           TSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIG FKSSKARNKLESILSMVSQKTLSR
Sbjct: 421 TSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGXFKSSKARNKLESILSMVSQKTLSR 480

Query: 576 RQNSSPGSKESISYALVDATKQPSFLKAFDNGEFKKSDKVLVAYKRRKGKFAAHLGEMTE 635
           RQNSSPGSKESISYALVDATKQPSFLKAFDNGEFKKSDKVLVAYKRRKGKFAAHLGEMTE
Sbjct: 481 RQNSSPGSKESISYALVDATKQPSFLKAFDNGEFKKSDKVLVAYKRRKGKFAAHLGEMTE 540

Query: 636 EEVEKFIGSVFNGDIPFTKTRQKPVFK 662
           EEVEKFIGSVFNGDIPFTKTRQKPVFK
Sbjct: 541 EEVEKFIGSVFNGDIPFTKTRQKPVFK 567

BLAST of MC05g1568.1 vs. NCBI nr
Match: XP_038905464.1 (dnaJ protein ERDJ3A isoform X1 [Benincasa hispida])

HSP 1 Score: 930 bits (2403), Expect = 0.0
Identity = 488/578 (84.43%), Postives = 524/578 (90.66%), Query Frame = 0

Query: 96  METRLAPIIFIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 155
           ME RLA I  IMA LFLFL SLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MEIRLASITLIMAFLFLFLCSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 156 KQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQ 215
           K+KGAQQKFEEINNAYEILSDEEKRKNFDMYG+EKGSPGFG G   DQGGGYTFFTNG  
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGNEKGSPGFGPGSSEDQGGGYTFFTNGAG 120

Query: 216 RQGQ-------WQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGG----GNF 275
           RQGQ       WQSTGG QG SQSFSFSFGGPSGSN FGFGMEDIFSNFFGGG    GNF
Sbjct: 121 RQGQQPFGPGQWQSTGG-QGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKDEGNF 180

Query: 276 GGFGGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIE 335
           G FG +SKAQ+GSRT S  S + INKQVF+KEIVDQGM WLLFP+TSSLKGVDHV+SIIE
Sbjct: 181 GSFGSHSKAQSGSRTSS--SIKVINKQVFKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIE 240

Query: 336 EVASSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVK 395
           EVASSLQGALKVGRIDC++ESSLCKDLGIYPHRTPRIFVYSYIKST+GSLVEYSGDVAVK
Sbjct: 241 EVASSLQGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVK 300

Query: 396 NLKSFCQEHLPRFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFS 455
           NL+SFCQEH PRFSQRV+L+QFDFS S+GG LPTLMLLSTKKDTPVIWRVLSGL+R++FS
Sbjct: 301 NLRSFCQEHFPRFSQRVNLKQFDFSSSDGGRLPTLMLLSTKKDTPVIWRVLSGLFRKQFS 360

Query: 456 FYDAEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGF 515
           FYD EV DVSDP++KKLGVDALPAIVGWLSNGERH+LR GINVKDL+SAIDDLSNL NGF
Sbjct: 361 FYDTEVTDVSDPSVKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGF 420

Query: 516 EKKNKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKARNKLESI 575
           EKKN+K  SRP SKT SDSAENQIPLLTGS+FD++CGEKTPVCVIGAF+SSKARNKLE I
Sbjct: 421 EKKNRKAVSRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELI 480

Query: 576 LSMVSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNGEFKKSDKVLVAYKRRKG 635
           L+MVSQKTLSRRQNS+PGSKE+ISYALVDA+KQPSFL AFD   FK SDK+L+AYKRRKG
Sbjct: 481 LNMVSQKTLSRRQNSTPGSKETISYALVDASKQPSFLNAFDGAGFKSSDKILIAYKRRKG 540

Query: 636 KFAAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 662
           KFAAHLGE+TEEEVEKFIGSV NGD+ FTKTRQKP+ K
Sbjct: 541 KFAAHLGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 575

BLAST of MC05g1568.1 vs. NCBI nr
Match: TYK24944.1 (dnaJ protein ERDJ3A [Cucumis melo var. makuwa])

HSP 1 Score: 906 bits (2341), Expect = 0.0
Identity = 474/575 (82.43%), Postives = 518/575 (90.09%), Query Frame = 0

Query: 96  METRLAPIIFIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 155
           M TRLA II ++A LFLF LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 156 KQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQ 215
           K+KGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKG+PGFG G  GDQGGGYTFFTNGG 
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 216 RQGQ-------WQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGG-GNFGGF 275
           RQGQ       WQSTGG QG SQSFSFSFGGPSGSN FGFGMEDIFSNFFGGG  + G F
Sbjct: 121 RQGQQPFGPGQWQSTGG-QGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKDKGSF 180

Query: 276 GGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEVA 335
           GG+SKAQ+G ++ S  S + INKQ+ +KEIVDQGM WLLFP+TSSLKGVDHV+SIIEEVA
Sbjct: 181 GGHSKAQSGPKSSS--SIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVA 240

Query: 336 SSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNLK 395
           S+LQGALKVGRIDC++ESS CKDLGIYPHRTPRIFVYSYIKS +GSLVEYSGD+ VK+LK
Sbjct: 241 STLQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYSGDIDVKSLK 300

Query: 396 SFCQEHLPRFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFYD 455
            FCQEH PRFSQRV+L+QFDFS SN G LPTLMLLSTKK+TPVIWRVLSGL+R+ F+FYD
Sbjct: 301 GFCQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFNFYD 360

Query: 456 AEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEKK 515
           AEV D SDP++KKLGVDALPAIVGWLSNGERH+LR GINVKDL+SAIDDLSNL NGFEKK
Sbjct: 361 AEVTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKK 420

Query: 516 NKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKARNKLESILSM 575
           N+K ASRPTSKT SDS ENQIPLLTGS+FD++CGEKTPVCVIGAF+SSKARNKLESIL M
Sbjct: 421 NRKAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESILKM 480

Query: 576 VSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNGEFKKSDKVLVAYKRRKGKFA 635
           VSQKTLSRRQNS+PGSKE+ISYALVDATKQ SFL AFD+  FK SDK+L+AYKRRKGKFA
Sbjct: 481 VSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFDSAGFKSSDKILIAYKRRKGKFA 540

Query: 636 AHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 662
           A+LGE+TEEEVEKFIGSV NGD+ FT TRQKP+ K
Sbjct: 541 AYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 572

BLAST of MC05g1568.1 vs. NCBI nr
Match: XP_008442391.1 (PREDICTED: dnaJ protein ERDJ3A [Cucumis melo] >KAA0044188.1 dnaJ protein ERDJ3A [Cucumis melo var. makuwa])

HSP 1 Score: 901 bits (2329), Expect = 0.0
Identity = 474/576 (82.29%), Postives = 518/576 (89.93%), Query Frame = 0

Query: 96  METRLAPIIFIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 155
           M TRLA II ++A LFLF LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 156 KQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQ 215
           K+KGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKG+PGFG G  GDQGGGYTFFTNGG 
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 216 RQGQ-------WQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGG-GNFGGF 275
           RQGQ       WQSTGG QG SQSFSFSFGGPSGSN FGFGMEDIFSNFFGGG  + G F
Sbjct: 121 RQGQQPFGPGQWQSTGG-QGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKDKGSF 180

Query: 276 GGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEVA 335
           GG+SKAQ+G ++ S  S + INKQ+ +KEIVDQGM WLLFP+TSSLKGVDHV+SIIEEVA
Sbjct: 181 GGHSKAQSGPKSSS--SIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVA 240

Query: 336 SSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNLK 395
           S+LQGALKVGRIDC++ESS CKDLGIYPHRTPRIFVYSYIKS +GSLVEYSGD+ VK+LK
Sbjct: 241 STLQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYSGDIDVKSLK 300

Query: 396 SFCQEHLPRFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFYD 455
            FCQEH PRFSQRV+L+QFDFS SN G LPTLMLLSTKK+TPVIWRVLSGL+R+ F+FYD
Sbjct: 301 GFCQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFNFYD 360

Query: 456 AEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEKK 515
           AEV D SDP++KKLGVDALPAIVGWLSNGERH+LR GINVKDL+SAIDDLSNL NGFEKK
Sbjct: 361 AEVTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKK 420

Query: 516 NKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKARNKLESILSM 575
           N+K ASRPTSKT SDS ENQIPLLTGS+FD++CGEKTPVCVIGAF+SSKARNKLESIL M
Sbjct: 421 NRKAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESILKM 480

Query: 576 VSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAF-DNGEFKKSDKVLVAYKRRKGKF 635
           VSQKTLSRRQNS+PGSKE+ISYALVDATKQ SFL AF D+  FK SDK+L+AYKRRKGKF
Sbjct: 481 VSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFVDSAGFKSSDKILIAYKRRKGKF 540

Query: 636 AAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 662
           AA+LGE+TEEEVEKFIGSV NGD+ FT TRQKP+ K
Sbjct: 541 AAYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 573

BLAST of MC05g1568.1 vs. NCBI nr
Match: XP_022935557.1 (dnaJ protein ERDJ3A [Cucurbita moschata] >KAG6580462.1 DnaJ protein ERDJ3A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017212.1 DnaJ protein ERDJ3A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 895 bits (2312), Expect = 0.0
Identity = 475/575 (82.61%), Postives = 517/575 (89.91%), Query Frame = 0

Query: 96  METRLAPIIFIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 155
           ME RL+P I IMASLFLFLLS EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MEIRLSPFILIMASLFLFLLS-EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 156 KQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQ 215
           K+K AQ KFEEINNAYEILSDEEKRKNFD+YGDEKGSPGFG G+ GDQGGG+TFFTN G+
Sbjct: 61  KKKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGR 120

Query: 216 RQGQ------WQSTGGGQGASQSFSFSFGGPSG-SNSFGFGMEDIFSNFFGGG-GNFGGF 275
           RQGQ      WQ TGG QG SQSFSFSFGGPSG SN FGFGMEDIFSNFFGGG  + G F
Sbjct: 121 RQGQQNFKPQWQGTGG-QGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNF 180

Query: 276 GGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEVA 335
           G + KAQ+GS++ S    + +NKQVF++E+V QG+ WLLFP TSSLKGVDHV+SIIE+VA
Sbjct: 181 GSHFKAQSGSKSSSI--IKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVA 240

Query: 336 SSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNLK 395
           SSLQGALK GRIDCE+ESSLCKDLGIYPHRTPRIFVYSYIKST GSLVEYSGDVAVK+LK
Sbjct: 241 SSLQGALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLK 300

Query: 396 SFCQEHLPRFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFYD 455
           SFCQEHLPRFS+RVDLRQFDFS +NGG LPTLMLLSTKKDTPVIWRVLSG+Y ++FSFYD
Sbjct: 301 SFCQEHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYD 360

Query: 456 AEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEKK 515
           AEV DVSDPT+KKLGVDALPAIVGWLSNGERH+LR GINVKDLRSAIDDLS L NGFEKK
Sbjct: 361 AEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKK 420

Query: 516 NKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKARNKLESILSM 575
           N+K AS+P SKT SDSA+NQIPLLTG +FD+ICG+ TPVCVIGAF+SSKARNKLE IL+M
Sbjct: 421 NRKAASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNM 480

Query: 576 VSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNGEFKKSDKVLVAYKRRKGKFA 635
           VSQKTLSRRQ S+PGSKESISY+LVDATKQPSFLKAFD+  FK SD++L+AYKRRK KFA
Sbjct: 481 VSQKTLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKEKFA 540

Query: 636 AHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 662
           AHLGEMTEEEVEKFIGSV NGDI FTKTRQKPV K
Sbjct: 541 AHLGEMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 571

BLAST of MC05g1568.1 vs. ExPASy TrEMBL
Match: A0A6J1CX10 (dnaJ protein ERDJ3A OS=Momordica charantia OX=3673 GN=LOC111015069 PE=4 SV=1)

HSP 1 Score: 1093 bits (2827), Expect = 0.0
Identity = 566/567 (99.82%), Postives = 566/567 (99.82%), Query Frame = 0

Query: 96  METRLAPIIFIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 155
           METRLAPIIFIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   METRLAPIIFIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 156 KQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQ 215
           KQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQ
Sbjct: 61  KQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQ 120

Query: 216 RQGQWQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGGGNFGGFGGNSKAQT 275
           RQGQWQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGGGNFGGFGGNSKAQT
Sbjct: 121 RQGQWQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGGGNFGGFGGNSKAQT 180

Query: 276 GSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEVASSLQGALK 335
           GSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEVASSLQGALK
Sbjct: 181 GSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEVASSLQGALK 240

Query: 336 VGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNLKSFCQEHLP 395
           VGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNLKSFCQEHLP
Sbjct: 241 VGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNLKSFCQEHLP 300

Query: 396 RFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFYDAEVNDVSD 455
           RFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFYDAEVNDVSD
Sbjct: 301 RFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFYDAEVNDVSD 360

Query: 456 PTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEKKNKKTASRP 515
           PTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEKKNKKTASRP
Sbjct: 361 PTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEKKNKKTASRP 420

Query: 516 TSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKARNKLESILSMVSQKTLSR 575
           TSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIG FKSSKARNKLESILSMVSQKTLSR
Sbjct: 421 TSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGXFKSSKARNKLESILSMVSQKTLSR 480

Query: 576 RQNSSPGSKESISYALVDATKQPSFLKAFDNGEFKKSDKVLVAYKRRKGKFAAHLGEMTE 635
           RQNSSPGSKESISYALVDATKQPSFLKAFDNGEFKKSDKVLVAYKRRKGKFAAHLGEMTE
Sbjct: 481 RQNSSPGSKESISYALVDATKQPSFLKAFDNGEFKKSDKVLVAYKRRKGKFAAHLGEMTE 540

Query: 636 EEVEKFIGSVFNGDIPFTKTRQKPVFK 662
           EEVEKFIGSVFNGDIPFTKTRQKPVFK
Sbjct: 541 EEVEKFIGSVFNGDIPFTKTRQKPVFK 567

BLAST of MC05g1568.1 vs. ExPASy TrEMBL
Match: A0A5D3DMX5 (DnaJ protein ERDJ3A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00600 PE=4 SV=1)

HSP 1 Score: 906 bits (2341), Expect = 0.0
Identity = 474/575 (82.43%), Postives = 518/575 (90.09%), Query Frame = 0

Query: 96  METRLAPIIFIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 155
           M TRLA II ++A LFLF LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 156 KQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQ 215
           K+KGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKG+PGFG G  GDQGGGYTFFTNGG 
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 216 RQGQ-------WQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGG-GNFGGF 275
           RQGQ       WQSTGG QG SQSFSFSFGGPSGSN FGFGMEDIFSNFFGGG  + G F
Sbjct: 121 RQGQQPFGPGQWQSTGG-QGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKDKGSF 180

Query: 276 GGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEVA 335
           GG+SKAQ+G ++ S  S + INKQ+ +KEIVDQGM WLLFP+TSSLKGVDHV+SIIEEVA
Sbjct: 181 GGHSKAQSGPKSSS--SIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVA 240

Query: 336 SSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNLK 395
           S+LQGALKVGRIDC++ESS CKDLGIYPHRTPRIFVYSYIKS +GSLVEYSGD+ VK+LK
Sbjct: 241 STLQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYSGDIDVKSLK 300

Query: 396 SFCQEHLPRFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFYD 455
            FCQEH PRFSQRV+L+QFDFS SN G LPTLMLLSTKK+TPVIWRVLSGL+R+ F+FYD
Sbjct: 301 GFCQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFNFYD 360

Query: 456 AEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEKK 515
           AEV D SDP++KKLGVDALPAIVGWLSNGERH+LR GINVKDL+SAIDDLSNL NGFEKK
Sbjct: 361 AEVTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKK 420

Query: 516 NKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKARNKLESILSM 575
           N+K ASRPTSKT SDS ENQIPLLTGS+FD++CGEKTPVCVIGAF+SSKARNKLESIL M
Sbjct: 421 NRKAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESILKM 480

Query: 576 VSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNGEFKKSDKVLVAYKRRKGKFA 635
           VSQKTLSRRQNS+PGSKE+ISYALVDATKQ SFL AFD+  FK SDK+L+AYKRRKGKFA
Sbjct: 481 VSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFDSAGFKSSDKILIAYKRRKGKFA 540

Query: 636 AHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 662
           A+LGE+TEEEVEKFIGSV NGD+ FT TRQKP+ K
Sbjct: 541 AYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 572

BLAST of MC05g1568.1 vs. ExPASy TrEMBL
Match: A0A5A7TMT4 (DnaJ protein ERDJ3A OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G005460 PE=4 SV=1)

HSP 1 Score: 901 bits (2329), Expect = 0.0
Identity = 474/576 (82.29%), Postives = 518/576 (89.93%), Query Frame = 0

Query: 96  METRLAPIIFIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 155
           M TRLA II ++A LFLF LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 156 KQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQ 215
           K+KGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKG+PGFG G  GDQGGGYTFFTNGG 
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 216 RQGQ-------WQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGG-GNFGGF 275
           RQGQ       WQSTGG QG SQSFSFSFGGPSGSN FGFGMEDIFSNFFGGG  + G F
Sbjct: 121 RQGQQPFGPGQWQSTGG-QGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKDKGSF 180

Query: 276 GGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEVA 335
           GG+SKAQ+G ++ S  S + INKQ+ +KEIVDQGM WLLFP+TSSLKGVDHV+SIIEEVA
Sbjct: 181 GGHSKAQSGPKSSS--SIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVA 240

Query: 336 SSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNLK 395
           S+LQGALKVGRIDC++ESS CKDLGIYPHRTPRIFVYSYIKS +GSLVEYSGD+ VK+LK
Sbjct: 241 STLQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYSGDIDVKSLK 300

Query: 396 SFCQEHLPRFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFYD 455
            FCQEH PRFSQRV+L+QFDFS SN G LPTLMLLSTKK+TPVIWRVLSGL+R+ F+FYD
Sbjct: 301 GFCQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFNFYD 360

Query: 456 AEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEKK 515
           AEV D SDP++KKLGVDALPAIVGWLSNGERH+LR GINVKDL+SAIDDLSNL NGFEKK
Sbjct: 361 AEVTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKK 420

Query: 516 NKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKARNKLESILSM 575
           N+K ASRPTSKT SDS ENQIPLLTGS+FD++CGEKTPVCVIGAF+SSKARNKLESIL M
Sbjct: 421 NRKAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESILKM 480

Query: 576 VSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAF-DNGEFKKSDKVLVAYKRRKGKF 635
           VSQKTLSRRQNS+PGSKE+ISYALVDATKQ SFL AF D+  FK SDK+L+AYKRRKGKF
Sbjct: 481 VSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFVDSAGFKSSDKILIAYKRRKGKF 540

Query: 636 AAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 662
           AA+LGE+TEEEVEKFIGSV NGD+ FT TRQKP+ K
Sbjct: 541 AAYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 573

BLAST of MC05g1568.1 vs. ExPASy TrEMBL
Match: A0A1S3B542 (dnaJ protein ERDJ3A OS=Cucumis melo OX=3656 GN=LOC103486271 PE=4 SV=1)

HSP 1 Score: 901 bits (2329), Expect = 0.0
Identity = 474/576 (82.29%), Postives = 518/576 (89.93%), Query Frame = 0

Query: 96  METRLAPIIFIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 155
           M TRLA II ++A LFLF LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 156 KQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQ 215
           K+KGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKG+PGFG G  GDQGGGYTFFTNGG 
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 216 RQGQ-------WQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGG-GNFGGF 275
           RQGQ       WQSTGG QG SQSFSFSFGGPSGSN FGFGMEDIFSNFFGGG  + G F
Sbjct: 121 RQGQQPFGPGQWQSTGG-QGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKDKGSF 180

Query: 276 GGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEVA 335
           GG+SKAQ+G ++ S  S + INKQ+ +KEIVDQGM WLLFP+TSSLKGVDHV+SIIEEVA
Sbjct: 181 GGHSKAQSGPKSSS--SIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVA 240

Query: 336 SSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNLK 395
           S+LQGALKVGRIDC++ESS CKDLGIYPHRTPRIFVYSYIKS +GSLVEYSGD+ VK+LK
Sbjct: 241 STLQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYSGDIDVKSLK 300

Query: 396 SFCQEHLPRFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFYD 455
            FCQEH PRFSQRV+L+QFDFS SN G LPTLMLLSTKK+TPVIWRVLSGL+R+ F+FYD
Sbjct: 301 GFCQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFNFYD 360

Query: 456 AEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEKK 515
           AEV D SDP++KKLGVDALPAIVGWLSNGERH+LR GINVKDL+SAIDDLSNL NGFEKK
Sbjct: 361 AEVTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKK 420

Query: 516 NKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKARNKLESILSM 575
           N+K ASRPTSKT SDS ENQIPLLTGS+FD++CGEKTPVCVIGAF+SSKARNKLESIL M
Sbjct: 421 NRKAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESILKM 480

Query: 576 VSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAF-DNGEFKKSDKVLVAYKRRKGKF 635
           VSQKTLSRRQNS+PGSKE+ISYALVDATKQ SFL AF D+  FK SDK+L+AYKRRKGKF
Sbjct: 481 VSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFVDSAGFKSSDKILIAYKRRKGKF 540

Query: 636 AAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 662
           AA+LGE+TEEEVEKFIGSV NGD+ FT TRQKP+ K
Sbjct: 541 AAYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 573

BLAST of MC05g1568.1 vs. ExPASy TrEMBL
Match: A0A0A0LDD2 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G730990 PE=4 SV=1)

HSP 1 Score: 895 bits (2312), Expect = 0.0
Identity = 467/575 (81.22%), Postives = 515/575 (89.57%), Query Frame = 0

Query: 96  METRLAPIIFIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 155
           METRLA IIF+MA LFLF LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   METRLASIIFVMAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 156 KQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQ 215
            +KGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKG+PGFG G  GDQGGGYTFFTNGG 
Sbjct: 61  NKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGPGSSGDQGGGYTFFTNGGG 120

Query: 216 RQGQ-------WQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGG-GNFGGF 275
           RQGQ       WQSTGG QG SQSFSFSFGGPSGSN FGFG EDIFSN FGGG    G F
Sbjct: 121 RQGQQPFGPGQWQSTGG-QGGSQSFSFSFGGPSGSNPFGFGAEDIFSNLFGGGFQGKGSF 180

Query: 276 GGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEVA 335
           GG+SKAQ+G ++ S  S + INK+  +KEIVDQGM WLLFP+ SSLKG+DHV+S IEEVA
Sbjct: 181 GGHSKAQSGPKSSS--SIKVINKKSLKKEIVDQGMTWLLFPTASSLKGLDHVQSTIEEVA 240

Query: 336 SSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNLK 395
           +SLQGALKVGRIDC++ESS CKDLGIYPHRTPRIFVYSYIKS++GSLVEYSGD+AVK+LK
Sbjct: 241 NSLQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSSEGSLVEYSGDIAVKSLK 300

Query: 396 SFCQEHLPRFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFYD 455
           SFCQEH PRFSQRV+L+QFDFS SN GGLPTLMLLSTKK+TPVIWRVLSGL+R++F+FYD
Sbjct: 301 SFCQEHFPRFSQRVNLKQFDFSSSNRGGLPTLMLLSTKKETPVIWRVLSGLFRKQFNFYD 360

Query: 456 AEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEKK 515
           AEV   SDP++KKLGVDALPAIVGWLSNGERH+LR GINVKDL+SAIDDLSNL NGFEKK
Sbjct: 361 AEVTYASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKK 420

Query: 516 NKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKARNKLESILSM 575
           N+K ASRP S+T SDS ENQIPLL GS+FD++CGEKTPVCVIGAF+SSKARNKLESIL+M
Sbjct: 421 NRKAASRPASRTQSDSGENQIPLLAGSNFDSVCGEKTPVCVIGAFRSSKARNKLESILNM 480

Query: 576 VSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNGEFKKSDKVLVAYKRRKGKFA 635
           VSQKTLSRRQNS+PGSKE++SYALVDATKQ SFL AFD+  FK SDK+L+AYKRRKGKFA
Sbjct: 481 VSQKTLSRRQNSTPGSKETVSYALVDATKQVSFLNAFDSAGFKSSDKILIAYKRRKGKFA 540

Query: 636 AHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 662
           A+L E+TEEEVEKFIGSV NGD+ FTKT QKP+ K
Sbjct: 541 AYLDEITEEEVEKFIGSVLNGDVTFTKTHQKPILK 572

BLAST of MC05g1568.1 vs. TAIR 10
Match: AT3G08970.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 650.6 bits (1677), Expect = 1.4e-186
Identity = 342/576 (59.38%), Postives = 437/576 (75.87%), Query Frame = 0

Query: 96  METRLAPIIFIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 155
           + TRLA  + ++++  L LL+++AK++DPYKVLGV ++A QREIQKAFHK SL+YHPDKN
Sbjct: 2   VRTRLAISVVLVST--LLLLNVKAKSVDPYKVLGVSKDAKQREIQKAFHKQSLKYHPDKN 61

Query: 156 KQKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGG- 215
           K KGAQ+KF EINNAYEILSDEEKRKN+D+YGDEKG PGF  G PG   GGY++ ++GG 
Sbjct: 62  KDKGAQEKFAEINNAYEILSDEEKRKNYDLYGDEKGQPGFDSGFPGG-NGGYSYSSSGGG 121

Query: 216 ---QRQGQWQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGGG----NFGGF 275
                 G WQ+ GGG G S+SFSFSFGGPS S SFGFGM+DIFS F GG       FGGF
Sbjct: 122 FNFGGPGGWQNMGGG-GGSKSFSFSFGGPSES-SFGFGMDDIFSMFSGGSSKGKEQFGGF 181

Query: 276 GGNSKAQTGSRTRSAPSTRAINKQVFRKEIVDQGMAWLLFPSTSSLKGVDHVESIIEEVA 335
           G +S A++ S++ +  + + IN QV++K++VDQGM WLL     S +G  + ESIIEEVA
Sbjct: 182 GSSSNAESKSKSSTVAAIKTINSQVYKKDVVDQGMTWLLLSYLPSQRGSQYHESIIEEVA 241

Query: 336 SSLQGALKVGRIDCEAESSLCKDLGIYPHRTPRIFVYSYIKSTQGSLVEYSGDVAVKNLK 395
            SLQGALKVGR++CE ESSLCK LGI P R PR+FVYSY  S + +L EY+ ++  K +K
Sbjct: 242 ESLQGALKVGRLNCETESSLCKQLGIVPRRAPRMFVYSYTSSGKATLAEYTEELVAKKVK 301

Query: 396 SFCQEHLPRFSQRVDLRQFDFSPSNGGGLPTLMLLSTKKDTPVIWRVLSGLYRRKFSFYD 455
           SFCQEHLPRFS+++DL  FD S  +    P ++LLSTKKDTPVIWRVLSGLY  +F FY+
Sbjct: 302 SFCQEHLPRFSKKIDLNTFDVSAVSSQKTPKVLLLSTKKDTPVIWRVLSGLYNGRFVFYN 361

Query: 456 AEVNDVSDPTMKKLGVDALPAIVGWLSNGERHILRAGINVKDLRSAIDDLSNLFNGFEKK 515
            EV+D SDP ++KLGVD  PAIVGWLSNGE+ +L+ GI VK+L+SA+ +L  L  G EKK
Sbjct: 362 TEVHDTSDPKIQKLGVDKFPAIVGWLSNGEKQVLKTGITVKNLKSAVQELGKLLEGLEKK 421

Query: 516 NKKTASRPTSKTPSDSAENQIPLLTGSDFDAICGEKTPVCVIGAFKSSKARNKLESILSM 575
           NKK +S+  +    + +  +IPLL+  +FD+ICGE TPVC+IGAF+SS  + KL+SI+S 
Sbjct: 422 NKKVSSKSQAGQAPNESSEKIPLLSRPNFDSICGENTPVCIIGAFRSSNGKEKLQSIMSK 481

Query: 576 VSQKTLSRRQNSSPGSKESISYALVDATKQPSFLKAFDNGEFK-KSDKVLVAYKRRKGKF 635
           VSQK+LSRRQ S+ GS++++SY+L+DATKQ +FL + D  EFK  SDK+L+AYK R+GKF
Sbjct: 482 VSQKSLSRRQASTTGSQDTVSYSLLDATKQSAFLSSLDKSEFKTSSDKLLIAYKPRRGKF 541

Query: 636 AAHLGEMTEEEVEKFIGSVFNGDIPFTKTRQKPVFK 663
           A   G+MT EEVEKF+ +V NGDI FTKTRQKP  K
Sbjct: 542 ATFKGDMTIEEVEKFVAAVLNGDIQFTKTRQKPQIK 572

BLAST of MC05g1568.1 vs. TAIR 10
Match: AT4G39960.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 86.7 bits (213), Expect = 7.9e-17
Identity = 55/117 (47.01%), Postives = 71/117 (60.68%), Query Frame = 0

Query: 123 DPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKN 182
           D Y VLGV +NA++ EI+ A+ KL+  YHPD NK  GA+ KF+EI+NAYEILSD+EKR  
Sbjct: 85  DFYSVLGVSKNATKAEIKSAYRKLARSYHPDVNKDAGAEDKFKEISNAYEILSDDEKRSL 144

Query: 183 FDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQRQGQWQSTGGGQGASQSFSFSFGG 240
           +D YG E G  G G G  GD    +  F +  +  G     GGG G+  S S +  G
Sbjct: 145 YDRYG-EAGVKGAGMGGMGDYSNPFDLFESLFEGMGGMGGMGGGMGSRGSRSRAIDG 200

BLAST of MC05g1568.1 vs. TAIR 10
Match: AT2G22360.1 (DNAJ heat shock family protein )

HSP 1 Score: 81.6 bits (200), Expect = 2.5e-15
Identity = 49/108 (45.37%), Postives = 68/108 (62.96%), Query Frame = 0

Query: 123 DPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKQKGAQQKFEEINNAYEILSDEEKRKN 182
           D Y VLGV +NA++ EI+ A+ KL+  YHPD NK  GA++KF+EI+NAYE+LSD+EK+  
Sbjct: 86  DYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEEKFKEISNAYEVLSDDEKKSL 145

Query: 183 FDMYGDE--KGSPGFGYGHPGDQGGGYTFFTNGGQRQGQWQSTGGGQG 229
           +D YG+   KG+ GFG    GD    +  F         ++  GGG G
Sbjct: 146 YDRYGEAGLKGAAGFG---NGDFSNPFDLF------DSLFEGFGGGMG 184

BLAST of MC05g1568.1 vs. TAIR 10
Match: AT5G03160.1 (homolog of mamallian P58IPK )

HSP 1 Score: 77.4 bits (189), Expect = 4.8e-14
Identity = 64/152 (42.11%), Postives = 79/152 (51.97%), Query Frame = 0

Query: 119 AKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN--KQKGAQQKFEEINNAYEILSD 178
           +K  D YK+LG+ R AS  EI+KA+ KL+LQ+HPDKN   ++ A+ KF EI  AYEIL D
Sbjct: 366 SKRKDWYKILGISRTASISEIKKAYKKLALQWHPDKNVGNREEAENKFREIAAAYEILGD 425

Query: 179 EEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGGQRQGQWQSTGGGQGASQSFSFS 238
           ++KR  FD   D +   G G       GGGY  F  GG         GGG    Q ++F 
Sbjct: 426 DDKRARFDRGEDLEDMGGGG-------GGGYNPFHGGG---------GGG----QQYTFH 481

Query: 239 FGGPSGSNSFGFGMEDIFSNFFGGGGNFGGFG 269
           F G                 F GGGG FGGFG
Sbjct: 486 FEG----------------GFPGGGGGFGGFG 481

BLAST of MC05g1568.1 vs. TAIR 10
Match: AT3G62600.1 (DNAJ heat shock family protein )

HSP 1 Score: 76.3 bits (186), Expect = 1.1e-13
Identity = 62/167 (37.13%), Postives = 87/167 (52.10%), Query Frame = 0

Query: 96  METRLAPIIFIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 155
           M  R + +  ++ +L   +  L  K+   Y VL V + AS  +I++A+ KL+L+YHPDKN
Sbjct: 1   MAIRWSELCIVLFALSYAICVLAGKSY--YDVLQVPKGASDEQIKRAYRKLALKYHPDKN 60

Query: 156 K-QKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGSPGFGYGHPGDQGGGYTFFTNGG 215
           +  + A +KF EINNAYE+LSDEEKR+ ++ YG+E               G   F  NGG
Sbjct: 61  QGNEEATRKFAEINNAYEVLSDEEKREIYNKYGEE---------------GLKQFSANGG 120

Query: 216 QRQGQWQSTGGGQGASQSFSFSFGGPSGSNSFGFGMEDIFSNFFGGG 262
           +        GGG G                  G  M+DIFS+FFGGG
Sbjct: 121 R--------GGGGG------------------GMNMQDIFSSFFGGG 124