MC03g0288.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0288.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	cysteine proteinase inhibitor 1-like
Location	MC03: 8599730 .. 8600248 (+)
Sequence length	519
RNA-Seq Expression	MC03g0288.1
Synteny	MC03g0288.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGCTATCAGCCGATCAAAGATTTGGATGCCCCATATGTTCAGGAAATCGCAAGGTATGCATGTATTGAGTACAACAGGAGACGAGGGAGTTCCTCTACGCCATATGTATACCAAAAAGTGGTGAGTGGGGAAAAACAGGTGGTGGAAGGAAGTAACTACAAGCTCATGTTAATGGTCACCATTGACAAACGAGCTCAGAAATGTGAGGCCATTGTGTATGACAAGCCATGGGAGAGCCACAGAAACCTTACATTTTTCCATCCCATCATGCATGTTTCTCCACCCCTAGTGATCGGCGGATATCAGCCGATCAAAAATTTGAGTGACCCATACGTTTCCGAAATCGGAAGGTGGGCAGTGATTGAGTACAACAGGATAAACCCTAGTACCTCTACACCATTTGTATTCAAGGAAGTGGTGAGTGGGGAAGAACAGGTGGTGGCTGGAACTAACTACAAGCTCATAATATACATCAAAAGCGGCGGTCTAATTCCCAAATATGAGGTCATCGTGTATGAC

mRNA sequence

GGCTATCAGCCGATCAAAGATTTGGATGCCCCATATGTTCAGGAAATCGCAAGGTATGCATGTATTGAGTACAACAGGAGACGAGGGAGTTCCTCTACGCCATATGTATACCAAAAAGTGGTGAGTGGGGAAAAACAGGTGGTGGAAGGAAGTAACTACAAGCTCATGTTAATGGTCACCATTGACAAACGAGCTCAGAAATGTGAGGCCATTGTGTATGACAAGCCATGGGAGAGCCACAGAAACCTTACATTTTTCCATCCCATCATGCATGTTTCTCCACCCCTAGTGATCGGCGGATATCAGCCGATCAAAAATTTGAGTGACCCATACGTTTCCGAAATCGGAAGGTGGGCAGTGATTGAGTACAACAGGATAAACCCTAGTACCTCTACACCATTTGTATTCAAGGAAGTGGTGAGTGGGGAAGAACAGGTGGTGGCTGGAACTAACTACAAGCTCATAATATACATCAAAAGCGGCGGTCTAATTCCCAAATATGAGGTCATCGTGTATGAC

Coding sequence (CDS)

GGCTATCAGCCGATCAAAGATTTGGATGCCCCATATGTTCAGGAAATCGCAAGGTATGCATGTATTGAGTACAACAGGAGACGAGGGAGTTCCTCTACGCCATATGTATACCAAAAAGTGGTGAGTGGGGAAAAACAGGTGGTGGAAGGAAGTAACTACAAGCTCATGTTAATGGTCACCATTGACAAACGAGCTCAGAAATGTGAGGCCATTGTGTATGACAAGCCATGGGAGAGCCACAGAAACCTTACATTTTTCCATCCCATCATGCATGTTTCTCCACCCCTAGTGATCGGCGGATATCAGCCGATCAAAAATTTGAGTGACCCATACGTTTCCGAAATCGGAAGGTGGGCAGTGATTGAGTACAACAGGATAAACCCTAGTACCTCTACACCATTTGTATTCAAGGAAGTGGTGAGTGGGGAAGAACAGGTGGTGGCTGGAACTAACTACAAGCTCATAATATACATCAAAAGCGGCGGTCTAATTCCCAAATATGAGGTCATCGTGTATGAC

Protein sequence

GYQPIKDLDAPYVQEIARYACIEYNRRRGSSSTPYVYQKVVSGEKQVVEGSNYKLMLMVTIDKRAQKCEAIVYDKPWESHRNLTFFHPIMHVSPPLVIGGYQPIKNLSDPYVSEIGRWAVIEYNRINPSTSTPFVFKEVVSGEEQVVAGTNYKLIIYIKSGGLIPKYEVIVYD

Homology

[Close]

BLAST of MC03g0288.1 vs. ExPASy Swiss-Prot
Match: Q10Q46 (Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210200 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.3e-12
Identity = 34/76 (44.74%), Postives = 49/76 (64.47%), Query Frame = 0


Query: 100 GYQPIKNLSDPYVSEIGRWAVIEYNRINPSTSTPFVFKEVVSGEEQVVAGTNYKLIIYIK 159
           G+ PIKN+ DP++ E+GRWA+ E NR++PS      F  V  GE+QVV+G NY+L I   
Sbjct: 22  GWSPIKNIDDPHIQELGRWAITENNRVSPSDE--LTFHRVTGGEQQVVSGMNYRLEIEAA 81

Query: 160 SGG--LIPKYEVIVYD 174
           SGG  +   Y  +V++
Sbjct: 82  SGGGDVTGSYGAVVFE 95

[Close]

BLAST of MC03g0288.1 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 69.7 bits (169), Expect = 3.7e-11
Identity = 34/89 (38.20%), Postives = 56/89 (62.92%), Query Frame = 0


Query: 1   GYQPIKDLDAPYVQEIARYACIEYNRRRGSSSTPYVYQKVVSGEKQVVEGSNYKLMLMVT 60
           G++PI++L++  VQ++A++A  E+N++   ++    YQ VV G  QVV G+NY+L++   
Sbjct: 31  GWRPIENLNSAEVQDVAQFAVSEHNKQ---ANDELQYQSVVRGYTQVVAGTNYRLVIAAK 90

Query: 61  IDKRAQKCEAIVYDKPWESHRNLTFFHPI 90
                   EA+V+DKPW   RNLT F  +
Sbjct: 91  DGAVVGNYEAVVWDKPWMHFRNLTSFRKV 116

[Close]

BLAST of MC03g0288.1 vs. ExPASy Swiss-Prot
Match: Q6TPK4 (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.8e-11
Identity = 34/89 (38.20%), Postives = 55/89 (61.80%), Query Frame = 0


Query: 1   GYQPIKDLDAPYVQEIARYACIEYNRRRGSSSTPYVYQKVVSGEKQVVEGSNYKLMLMVT 60
           G++PI+ L++  VQ++A++A  E+N++   ++    YQ VV G  QVV G+NY+L++   
Sbjct: 31  GWRPIESLNSAEVQDVAQFAVSEHNKQ---ANDELQYQSVVRGYTQVVAGTNYRLVIAAK 90

Query: 61  IDKRAQKCEAIVYDKPWESHRNLTFFHPI 90
                   EA+V+DKPW   RNLT F  +
Sbjct: 91  DGAVVGNYEAVVWDKPWMHFRNLTSFRKV 116

[Close]

BLAST of MC03g0288.1 vs. ExPASy Swiss-Prot
Match: Q41916 (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 68.9 bits (167), Expect = 6.2e-11
Identity = 37/85 (43.53%), Postives = 53/85 (62.35%), Query Frame = 0


Query: 90  MHVSPPLVIGGYQPIKNLSDPYVSEIGRWAVIEYNRINPSTSTPFVFKEVVSGEEQVVAG 149
           ++ S    +GG+ PI N++DP V EIG +AV EYN+    + +   F+ VVSGE QVV+G
Sbjct: 20  LYASAAARVGGWSPISNVTDPQVVEIGEFAVSEYNK---RSESGLKFETVVSGETQVVSG 79

Query: 150 TNYKLIIYIKSG-GLIPKYEVIVYD 174
           TNY+L +    G G+   Y  IV+D
Sbjct: 80  TNYRLKVAANDGDGVSKNYLAIVWD 101

[Close]

BLAST of MC03g0288.1 vs. ExPASy Swiss-Prot
Match: Q10Q47 (Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210100 PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.0e-08
Identity = 31/77 (40.26%), Postives = 49/77 (63.64%), Query Frame = 0


Query: 98  IGGYQPIKNLSDPYVSEIGRWAVIEYNRINPSTSTPFVFKEVVSGEEQVVAGTNYKLIIY 157
           +G ++P+ +++DP+V E+GRWAV E +R     +    F+ V  GE+QVVAG NY+L + 
Sbjct: 27  VGSWEPV-DINDPHVQELGRWAVAEEDR--GVAAGGLTFERVTDGEKQVVAGVNYRLTLE 86

Query: 158 IKSGGLIP-KYEVIVYD 174
             S G    +YE +VY+
Sbjct: 87  ASSSGAKDGRYEAVVYE 100

[Close]

BLAST of MC03g0288.1 vs. NCBI nr
Match: XP_022147176.1 (cysteine proteinase inhibitor 1-like [Momordica charantia])

HSP 1 Score: 248 bits (633), Expect = 6.24e-81
Identity = 124/172 (72.09%), Postives = 143/172 (83.14%), Query Frame = 0


Query: 2   YQPIKDLDAPYVQEIARYACIEYNRRRGSSSTPYVYQKVVSGEKQVVEGSNYKLMLMVTI 61
           Y+PI D++APY+QEIARYACIEYNRR+G SSTPYVYQKVVSGE+QVV G+NYKL+L VTI
Sbjct: 17  YEPIDDVNAPYIQEIARYACIEYNRRQGHSSTPYVYQKVVSGERQVVSGTNYKLILNVTI 76

Query: 62  DKRAQKCEAIVYDKPWESHRNLTFFHPIMHVSPPLVIGGYQPIKNLSDPYVSEIGRWAVI 121
           +K+    E IVYDKPWES+RNLT F+P +       +GGYQPIKNLSDPY+SEIGR+A I
Sbjct: 77  NKQIHNYEVIVYDKPWESYRNLTSFNPFVF------LGGYQPIKNLSDPYISEIGRYACI 136

Query: 122 EYNRINPSTSTPFVFKEVVSGEEQVVAGTNYKLIIYIKSGGLIPKYEVIVYD 173
           EYNR NP+  TP VFK+VVSGEEQVVAGTNYKLI+Y K G L   YEVIVYD
Sbjct: 137 EYNRKNPNP-TPLVFKKVVSGEEQVVAGTNYKLILYTKRGSLNLNYEVIVYD 181

[Close]

BLAST of MC03g0288.1 vs. NCBI nr
Match: MQM02766.1 (hypothetical protein [Colocasia esculenta])

HSP 1 Score: 159 bits (403), Expect = 1.16e-44
Identity = 84/173 (48.55%), Postives = 113/173 (65.32%), Query Frame = 0


Query: 1   GYQPIKDLDAPYVQEIARYACIEYNRRRGSSSTPYVYQKVVSGEKQVVEGSNYKLMLMVT 60
           GY+PI+D+D PYVQEI ++   E+N++ G      V+ +VVSGE+QVV G+NYKL++   
Sbjct: 6   GYRPIEDIDDPYVQEIGKFVVEEHNKQAGQG---LVFVRVVSGEQQVVSGTNYKLVVAAE 65

Query: 61  IDKRAQKCEAIVYDKPWESHRNLTFFHPIMHVSPPLVIGGYQPIKNLSDPYVSEIGRWAV 120
                 K EA+VYDKPWE+ R LT F  ++       +GGY+PI N+ DP+V EI  +AV
Sbjct: 66  DAGATNKYEAVVYDKPWENFRELTSF-ALVPEQVVKQVGGYRPIPNIKDPHVQEIAEFAV 125

Query: 121 IEYNRINPSTSTPFVFKEVVSGEEQVVAGTNYKLIIYIKSGGLIPKYEVIVYD 173
            EYN+         VF  VVSGE+QVVAGTNYKL+I  +  G+I  YE +VYD
Sbjct: 126 AEYNK---QVGKALVFISVVSGEQQVVAGTNYKLVIEAEDCGVIKTYETVVYD 171

[Close]

BLAST of MC03g0288.1 vs. NCBI nr
Match: MQM15186.1 (hypothetical protein [Colocasia esculenta])

HSP 1 Score: 164 bits (414), Expect = 4.17e-43
Identity = 87/173 (50.29%), Postives = 113/173 (65.32%), Query Frame = 0


Query: 1   GYQPIKDLDAPYVQEIARYACIEYNRRRGSSSTPYVYQKVVSGEKQVVEGSNYKLMLMVT 60
           GYQPI D+D PYVQEI ++A  E+N++ G      V+ +VVSGE+QVV G+NYKL++   
Sbjct: 102 GYQPIADIDDPYVQEIGKFAVEEHNKQAGQG---LVFVRVVSGEQQVVAGTNYKLVIAAE 161

Query: 61  IDKRAQKCEAIVYDKPWESHRNLTFFHPIMHVSPPLVIGGYQPIKNLSDPYVSEIGRWAV 120
                 K EA+VYDKPWE+ R LT F  IM       +GGYQPI N+ DP+V EI ++AV
Sbjct: 162 DAGATNKYEAVVYDKPWENFRELTSF-AIMPKQVVKQVGGYQPIPNIKDPHVLEIAKFAV 221

Query: 121 IEYNRINPSTSTPFVFKEVVSGEEQVVAGTNYKLIIYIKSGGLIPKYEVIVYD 173
            EYN+         VF  VVSGE+QVVAGTNYKL+I  +  G+   YE ++YD
Sbjct: 222 AEYNK---QAGKALVFVSVVSGEQQVVAGTNYKLVIVAEDCGVFKMYEAVLYD 267

[Close]

BLAST of MC03g0288.1 vs. NCBI nr
Match: MQM16017.1 (hypothetical protein [Colocasia esculenta])

HSP 1 Score: 151 bits (382), Expect = 6.74e-39
Identity = 82/177 (46.33%), Postives = 112/177 (63.28%), Query Frame = 0


Query: 2   YQPIKDLDAPYVQEIARYACIEYNRRRGSSSTPYVYQKVVSGEKQVVEGSNYKLMLMVTI 61
           YQPI +L  P+VQEIA +A  +YN++ G +    V+  VVSGE+QV+ G+NYKL +    
Sbjct: 234 YQPIPNLKDPHVQEIAEFAVAKYNKQEGKA---LVFVSVVSGEQQVIAGTNYKLAIEAED 293

Query: 62  DKRAQKCEAIVYDKPWESHRNLTFFHP-----IMHVSPPLVIGGYQPIKNLSDPYVSEIG 121
               +  EA+VYDK WE  + LT F P     +   S  + +GGYQPI+N+ DP++ EIG
Sbjct: 294 CGVIKTYEAVVYDKSWEKIKELTSFDPMPERVVKQRSFEMRVGGYQPIRNVKDPHIQEIG 353

Query: 122 RWAVIEYNRINPSTSTPFVFKEVVSGEEQVVAGTNYKLIIYIKSGGLIPKYEVIVYD 173
           ++AV E+N+         VF  VVSGE+QVVAGTNYKL+I  K  G+   YE +VYD
Sbjct: 354 KFAVEEHNK---QAGKALVFVRVVSGEQQVVAGTNYKLVIEAKDAGVTNTYEAVVYD 404

[Close]

BLAST of MC03g0288.1 vs. NCBI nr
Match: XP_020244461.1 (cysteine proteinase inhibitor 1-like [Asparagus officinalis])

HSP 1 Score: 135 bits (339), Expect = 4.79e-36
Identity = 76/177 (42.94%), Postives = 111/177 (62.71%), Query Frame = 0


Query: 1   GYQPIKDLDAPYVQEIARYACIEYNRRRGSSSTPYVYQKVVSGEKQVVEGSNYKLMLMVT 60
           GY PIK+L  P++ EI ++A  E+N+    ++T  V+ KV+ GE QVV G NYKL++   
Sbjct: 40  GYGPIKNLSDPHLGEIGKFAVSEHNKE---ANTKLVFTKVIKGESQVVAGINYKLVIEAK 99

Query: 61  I-DKRAQKCEAIVYDKPWESHRNLTFFHPIMHVSPPLVIGGYQPIKNLSDPYVSEIGRWA 120
             +    + E IVY++ WE +  LT F+P+       + GGY PIKNLSDP++ EIG++A
Sbjct: 100 DQNNEVGRYEVIVYERAWEKYMELTSFNPVG------IPGGYIPIKNLSDPHLVEIGKFA 159

Query: 121 VIEYNRINPSTSTPFVFKEVVSGEEQVVAGTNYKLIIYIKS---GGLIPKYEVIVYD 173
           V E+N+     +T  VF +V+ GE QVVAG NYKL+I  K       + +YEVIV++
Sbjct: 160 VSEHNK---EANTKLVFTKVIKGESQVVAGINYKLVIEAKDITRNNKVGRYEVIVWE 204

[Close]

BLAST of MC03g0288.1 vs. ExPASy TrEMBL
Match: A0A6J1D1J8 (cysteine proteinase inhibitor 1-like OS=Momordica charantia OX=3673 GN=LOC111016188 PE=4 SV=1)

HSP 1 Score: 248 bits (633), Expect = 3.02e-81
Identity = 124/172 (72.09%), Postives = 143/172 (83.14%), Query Frame = 0


Query: 2   YQPIKDLDAPYVQEIARYACIEYNRRRGSSSTPYVYQKVVSGEKQVVEGSNYKLMLMVTI 61
           Y+PI D++APY+QEIARYACIEYNRR+G SSTPYVYQKVVSGE+QVV G+NYKL+L VTI
Sbjct: 17  YEPIDDVNAPYIQEIARYACIEYNRRQGHSSTPYVYQKVVSGERQVVSGTNYKLILNVTI 76

Query: 62  DKRAQKCEAIVYDKPWESHRNLTFFHPIMHVSPPLVIGGYQPIKNLSDPYVSEIGRWAVI 121
           +K+    E IVYDKPWES+RNLT F+P +       +GGYQPIKNLSDPY+SEIGR+A I
Sbjct: 77  NKQIHNYEVIVYDKPWESYRNLTSFNPFVF------LGGYQPIKNLSDPYISEIGRYACI 136

Query: 122 EYNRINPSTSTPFVFKEVVSGEEQVVAGTNYKLIIYIKSGGLIPKYEVIVYD 173
           EYNR NP+  TP VFK+VVSGEEQVVAGTNYKLI+Y K G L   YEVIVYD
Sbjct: 137 EYNRKNPNP-TPLVFKKVVSGEEQVVAGTNYKLILYTKRGSLNLNYEVIVYD 181

[Close]

BLAST of MC03g0288.1 vs. ExPASy TrEMBL
Match: V4T622 (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10002632mg PE=4 SV=1)

HSP 1 Score: 128 bits (322), Expect = 2.63e-34
Identity = 68/173 (39.31%), Postives = 109/173 (63.01%), Query Frame = 0


Query: 1   GYQPIKDLDAPYVQEIARYACIEYNRRRGSSSTPYVYQKVVSGEKQVVEGSNYKLMLMVT 60
           G++PI+D    +V EI ++A  EYN+R   S +   ++ V  GE QVV G+NY+L+L+V 
Sbjct: 4   GWKPIEDPKEKHVMEIGQFAVTEYNKR---SKSALKFESVEKGETQVVSGTNYRLILVVK 63

Query: 61  IDKRAQKCEAIVYDKPWESHRNLTFFHPIMHVSPPLVIGGYQPIKNLSDPYVSEIGRWAV 120
                +K EA+V +KPWE  ++LT F P+       ++GG++PI++  + +V EIG++AV
Sbjct: 64  DGPSTKKFEAVVLEKPWEHFKSLTSFKPMP------MLGGWKPIEDPKEKHVMEIGQFAV 123

Query: 121 IEYNRINPSTSTPFVFKEVVSGEEQVVAGTNYKLIIYIKSGGLIPKYEVIVYD 173
            EYN+ +        F+ V  GE QVV+GTNY+LI+ +K G    K+E +V++
Sbjct: 124 TEYNKRSKGA---LKFESVEKGETQVVSGTNYRLILVVKDGPSTKKFEAVVWE 164

[Close]

BLAST of MC03g0288.1 vs. ExPASy TrEMBL
Match: A0A4S8KBT4 (Uncharacterized protein OS=Musa balbisiana OX=52838 GN=C4D60_Mb04t13670 PE=3 SV=1)

HSP 1 Score: 125 bits (315), Expect = 7.53e-33
Identity = 73/176 (41.48%), Postives = 110/176 (62.50%), Query Frame = 0


Query: 1   GYQPIKDLDAPYVQEIARYACIEYNRRRGSSSTPYVYQKVVSGEKQVVEGSNYKLMLMVT 60
           G+  I+DL  P+V+EIA +A  E+N+   ++ T    +KV  GE QVV G+NY+L+L V 
Sbjct: 34  GWTAIEDLKDPHVREIAEFAVSEHNKLEKTNLT---LRKVEGGETQVVAGTNYRLVLEVR 93

Query: 61  IDKRAQ-KCEAIVYDKPWESHRNLTFFHPIMHVSPPLVIGGYQPIKNLSDPYVSEIGRWA 120
               A  + EA+V++KPWE+ + L  FH ++      ++GG+ PIKN+SDP V EI  +A
Sbjct: 94  DGSGASARYEAVVWEKPWENFKQLASFHKLV------IVGGWTPIKNISDPDVGEIAEFA 153

Query: 121 VIEYNRINPSTSTPFVFKEVVSGEEQVVAGTNYKLIIYIKSGGL--IPKYEVIVYD 173
           + E+N    S  T     +VV GE QVVAG NYKL++  K GG+  + +YE +V++
Sbjct: 154 IAEHNHEAGSNLT---LCKVVKGETQVVAGINYKLVLEAKDGGVGVMSEYEAVVWE 197

[Close]

BLAST of MC03g0288.1 vs. ExPASy TrEMBL
Match: A0A444FRQ3 (Uncharacterized protein OS=Ensete ventricosum OX=4639 GN=GW17_00010346 PE=3 SV=1)

HSP 1 Score: 122 bits (306), Expect = 2.88e-31
Identity = 72/176 (40.91%), Postives = 107/176 (60.80%), Query Frame = 0


Query: 1   GYQPIKDLDAPYVQEIARYACIEYNRRRGSSSTPYVYQKVVSGEKQVVEGSNYKLMLMVT 60
           G+  I+DL  P+V+EIA +A  E+N+   ++ T    +KV  GE QVV G NY+L+L V 
Sbjct: 55  GWTAIEDLKDPHVREIAEFAVSEHNKLEKTNLT---LRKVEGGESQVVAGMNYRLVLEVR 114

Query: 61  IDKRAQ-KCEAIVYDKPWESHRNLTFFHPIMHVSPPLVIGGYQPIKNLSDPYVSEIGRWA 120
               A  + EA+V++K WE  R LT F  ++      ++GG+ PI+N+ DP V+EI  +A
Sbjct: 115 DGSGASVRYEAVVWEKTWEHFRELTSFRKLV------IVGGWTPIENIGDPDVAEIAEFA 174

Query: 121 VIEYNRINPSTSTPFVFKEVVSGEEQVVAGTNYKLIIYIKSGG--LIPKYEVIVYD 173
           V E+N       T     +VV GE QVVAGTNYKL++  K GG  ++ +YE +V++
Sbjct: 175 VAEHNH---EAGTNLKLCKVVKGETQVVAGTNYKLVLGAKDGGAGVVSEYEAVVWE 218

[Close]

BLAST of MC03g0288.1 vs. ExPASy TrEMBL
Match: A0A426Z5E1 (Uncharacterized protein OS=Ensete ventricosum OX=4639 GN=B296_00014443 PE=3 SV=1)

HSP 1 Score: 122 bits (307), Expect = 3.09e-31
Identity = 73/176 (41.48%), Postives = 106/176 (60.23%), Query Frame = 0


Query: 1   GYQPIKDLDAPYVQEIARYACIEYNRRRGSSSTPYVYQKVVSGEKQVVEGSNYKLMLMVT 60
           G+  I+DL  P+V+EIA +A  E+N    ++ T    +KV  GE QVV G NY+L+L V 
Sbjct: 71  GWTAIEDLKDPHVREIAEFAVSEHNMLEKTNLT---LRKVEGGESQVVAGMNYRLVLEVR 130

Query: 61  IDKRAQ-KCEAIVYDKPWESHRNLTFFHPIMHVSPPLVIGGYQPIKNLSDPYVSEIGRWA 120
               A  + EA+V++K WE  R LT +  ++     L +GG+ PIKN+SDP V EI  +A
Sbjct: 131 DGSGASARYEAVVWEKTWEHFRKLTSYRKLV-----LAVGGWTPIKNISDPDVGEIAEFA 190

Query: 121 VIEYNRINPSTSTPFVFKEVVSGEEQVVAGTNYKLIIYIKSGGL--IPKYEVIVYD 173
           V E+N      +T     +VV GE QVVAG NYKL++  K GG+  + +YE +V++
Sbjct: 191 VAEHNH---EAATNLTLCKVVKGETQVVAGINYKLVLEAKDGGVGVVSEYEAVVWE 235

[Close]

BLAST of MC03g0288.1 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 68.9 bits (167), Expect = 4.4e-12
Identity = 37/85 (43.53%), Postives = 53/85 (62.35%), Query Frame = 0


Query: 90  MHVSPPLVIGGYQPIKNLSDPYVSEIGRWAVIEYNRINPSTSTPFVFKEVVSGEEQVVAG 149
           ++ S    +GG+ PI N++DP V EIG +AV EYN+    + +   F+ VVSGE QVV+G
Sbjct: 20  LYASAAARVGGWSPISNVTDPQVVEIGEFAVSEYNK---RSESGLKFETVVSGETQVVSG 79

Query: 150 TNYKLIIYIKSG-GLIPKYEVIVYD 174
           TNY+L +    G G+   Y  IV+D
Sbjct: 80  TNYRLKVAANDGDGVSKNYLAIVWD 101

[Close]

BLAST of MC03g0288.1 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 7.8e-09
Identity = 32/77 (41.56%), Postives = 46/77 (59.74%), Query Frame = 0


Query: 98  IGGYQPIKNLSDPYVSEIGRWAVIEYNRINPSTSTPFVFKEVVSGEEQVVAGTNYKLIIY 157
           +G  +PIKN+SDP V  + ++A+ E+N+    +    VF +VV G  QVV+GT Y L I 
Sbjct: 29  LGSRKPIKNVSDPDVVAVAKYAIEEHNK---ESKEKLVFVKVVEGTTQVVSGTKYDLKIA 88

Query: 158 IK-SGGLIPKYEVIVYD 174
            K  GG I  YE +V +
Sbjct: 89  AKDGGGKIKNYEAVVVE 102

[Close]

BLAST of MC03g0288.1 vs. TAIR 10
Match: AT2G40880.1 (cystatin A )

HSP 1 Score: 41.6 bits (96), Expect = 7.6e-04
Identity = 23/78 (29.49%), Postives = 41/78 (52.56%), Query Frame = 0


Query: 96  LVIGGYQPIK-NLSDPYVSEIGRWAVIEYNRINPSTSTPFVFKEVVSGEEQVVAGTNYKL 155
           +++GG   ++ N +   +  + R+A+ E+N+     +    FK++V   EQVVAGT Y L
Sbjct: 33  MMLGGVHDLRGNQNSGEIESLARFAIQEHNK---QQNKILEFKKIVKAREQVVAGTMYHL 92

Query: 156 IIYIKSGGLIPKYEVIVY 173
            +  K G     +E  V+
Sbjct: 93  TLEAKEGDQTKNFEAKVW 107

The following BLAST results are available for this feature:

BLAST of MC03g0288.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q10Q46	3.3e-12	44.74	Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03...	[more]
P86472	3.7e-11	38.20	Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841...	[more]
Q6TPK4	4.8e-11	38.20	Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1	[more]
Q41916	6.2e-11	43.53	Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=...	[more]
Q10Q47	1.0e-08	40.26	Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=3994...	[more]

BLAST of MC03g0288.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147176.1	6.24e-81	72.09	cysteine proteinase inhibitor 1-like [Momordica charantia]	[more]
MQM02766.1	1.16e-44	48.55	hypothetical protein [Colocasia esculenta]	[more]
MQM15186.1	4.17e-43	50.29	hypothetical protein [Colocasia esculenta]	[more]
MQM16017.1	6.74e-39	46.33	hypothetical protein [Colocasia esculenta]	[more]
XP_020244461.1	4.79e-36	42.94	cysteine proteinase inhibitor 1-like [Asparagus officinalis]	[more]

BLAST of MC03g0288.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D1J8	3.02e-81	72.09	cysteine proteinase inhibitor 1-like OS=Momordica charantia OX=3673 GN=LOC111016...	[more]
V4T622	2.63e-34	39.31	Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10002632mg PE=4 ...	[more]
A0A4S8KBT4	7.53e-33	41.48	Uncharacterized protein OS=Musa balbisiana OX=52838 GN=C4D60_Mb04t13670 PE=3 SV=...	[more]
A0A444FRQ3	2.88e-31	40.91	Uncharacterized protein OS=Ensete ventricosum OX=4639 GN=GW17_00010346 PE=3 SV=1	[more]
A0A426Z5E1	3.09e-31	41.48	Uncharacterized protein OS=Ensete ventricosum OX=4639 GN=B296_00014443 PE=3 SV=1	[more]

BLAST of MC03g0288.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47550.1	4.4e-12	43.53	Cystatin/monellin superfamily protein	[more]
AT4G16500.1	7.8e-09	41.56	Cystatin/monellin superfamily protein	[more]
AT2G40880.1	7.6e-04	29.49	cystatin A	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.10		coord: 1..93 e-value: 7.4E-22 score: 79.4 coord: 95..173 e-value: 1.1E-17 score: 66.0
None	No IPR available	PANTHER	PTHR47364	CYSTEINE PROTEINASE INHIBITOR 5	coord: 1..90 coord: 96..173
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 1..86
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 92..172
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 104..172 e-value: 3.5E-16 score: 59.3 coord: 5..88 e-value: 2.9E-20 score: 72.4
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 99..161 e-value: 3.94706E-7 score: 44.3872
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 6..86 e-value: 3.91337E-9 score: 49.78

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC03g0288	MC03g0288	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC03g0288.1-cds	MC03g0288.1-cds-MC03:8599730..8600248	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC03g0288.1	MC03g0288.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity