MC02g1298.1 (mRNA) Bitter gourd (Dali-11) v1

BLAST of MC02g1298.1 vs. NCBI nr
Match: XP_038904899.1 (uncharacterized protein LOC120091119 isoform X2 [Benincasa hispida])

HSP 1 Score: 118 bits (295), Expect = 5.62e-27
Identity = 58/156 (37.18%), Postives = 94/156 (60.26%), Query Frame = 0

Query: 55  LSFTRDRILKSSELSVRWRKTGNFHIKLEKWSPYLHNRKEFIQVYGGCL---DLPLYYWS 114
           ++  R+ + +   +S +W+K G+FH+K E+W+  +H R  +++ YGG +   +LPL YW 
Sbjct: 207 VTMERESLDELVNISGKWQKFGSFHLKFERWNNSIHGRPAYVRGYGGWISIKNLPLDYWC 266

Query: 115 RSTFEAIGVHFGGLLKISTKTLNMLEASEAYIKVKPNLCGFIPATVTIKDEKCGSFEVR- 174
           + TFEAIG +FGGL  I+ + LN+++  +A IKVK NLCGF+PAT+ + +EK GS  +  
Sbjct: 267 KQTFEAIGKYFGGLESIAMEILNLIKVLDAIIKVKENLCGFVPATIEVSNEKRGSIYLNF 326

Query: 175 --VFAKNPPDSATAFDPNIDIELLSNPLDVARFRRI 204
             +   NPP          D    +NP+D+ R   +
Sbjct: 327 GDISTSNPPSKVKGDLFESDF---TNPIDLIRLNEV 359

BLAST of MC02g1298.1 vs. NCBI nr
Match: KAA0041398.1 (hypothetical protein E6C27_scaffold206G00440 [Cucumis melo var. makuwa])

HSP 1 Score: 108 bits (270), Expect = 3.34e-23
Identity = 63/177 (35.59%), Postives = 96/177 (54.24%), Query Frame = 0

Query: 3   VQKEIEVLDLDWNHLMVVSKSCAQDDWVAIAXALKE----KFSLDCVINPIMVDKILSFT 62
           ++KE    D+DW   +++S+ C  DDW  I   L+E    K S  C + P   DK L F 
Sbjct: 203 IRKEERKNDIDWEKTIILSRRCFHDDWDKIIDRLREQTDKKESCFCYV-PFHADKALLFI 262

Query: 63  RDRIL-KSSELSVRWRKTGNFHIKLEKWSPYLHNRKEFIQVYGGCL---DLPLYYWSRST 122
           +D+ L K    +  W   G F++K EKWS  +H   + I  YGG      +PL+ W+ +T
Sbjct: 263 KDKDLAKLLCKNNGWTTVGPFYVKFEKWSKSVHADTKVIPSYGGWTRFRGIPLHIWNLNT 322

Query: 123 FEAIGVHFGGLLKISTKTLNMLEASEAYIKVKPNLCGFIPATVTIKDEKCGSFEVRV 171
           F  IG   GG +  + +++N +E +EA IKVK N  GF+PA + I DE+   F +++
Sbjct: 323 FIHIGEACGGFIDAAPESVNKIELTEALIKVKENYTGFLPAFIQIHDEEGHVFIIQI 378

BLAST of MC02g1298.1 vs. NCBI nr
Match: KAA0039949.1 (hypothetical protein E6C27_scaffold122G002290 [Cucumis melo var. makuwa] >TYK24554.1 hypothetical protein E5676_scaffold266G00990 [Cucumis melo var. makuwa])

HSP 1 Score: 108 bits (270), Expect = 3.56e-23
Identity = 62/175 (35.43%), Postives = 94/175 (53.71%), Query Frame = 0

Query: 3   VQKEIEVLDLDWNHLMVVSKSCAQDDWVAIAXALKEKFSL-DCVIN--PIMVDKILSFTR 62
           ++KE +  D+DW   +++S+ C  DDW  I   LK++    D      P   DK L F +
Sbjct: 209 IRKEEKKNDIDWEKSIILSRRCFHDDWAKIIDRLKDQTDKKDSCFRYIPFHADKALLFIK 268

Query: 63  DRILKSSEL-SVRWRKTGNFHIKLEKWSPYLHNRKEFIQVYGGCL---DLPLYYWSRSTF 122
           D+ L +    ++ W   G F++K EKWS   H   + I  YGG      +PL+ W+ +TF
Sbjct: 269 DKELANLLCKNIGWTTVGPFYVKFEKWSKNAHADTKVIPSYGGWTRFRGIPLHIWNLNTF 328

Query: 123 EAIGVHFGGLLKISTKTLNMLEASEAYIKVKPNLCGFIPATVTIKDEKCGSFEVR 170
             IG   GG +  + +++N LE +EA IKVK N  GF+PA + I DE+   F V+
Sbjct: 329 IQIGEACGGFIDAAPESINKLELTEALIKVKENYTGFLPAFIQIHDEEGQDFIVQ 383

BLAST of MC02g1298.1 vs. NCBI nr
Match: TYJ98837.1 (putative 3,4-dihydroxy-2-butanone kinase isoform X5 [Cucumis melo var. makuwa])

HSP 1 Score: 102 bits (254), Expect = 4.76e-22
Identity = 50/107 (46.73%), Postives = 67/107 (62.62%), Query Frame = 0

Query: 61  RILKSSELSVRWRKTGNFHIKLEKWSPYLHNRKEFIQVYGGCL---DLPLYYWSRSTFEA 120
           R+    +   +W+  GNFH+K+E+W    H+     + YGG L   +LPL YW RST E 
Sbjct: 178 RVCLGKDQQGKWQTWGNFHLKIERWDNLKHSWPLVQKGYGGWLKIKNLPLDYWCRSTLEV 237

Query: 121 IGVHFGGLLKISTKTLNMLEASEAYIKVKPNLCGFIPATVTIKDEKC 164
           IG HF GL  I+ +TLN+   SEA I+VK NLCGF+P+T+ I D KC
Sbjct: 238 IGDHFRGLTDIAFETLNLTNVSEARIQVKKNLCGFVPSTIEITDLKC 284

BLAST of MC02g1298.1 vs. NCBI nr
Match: KAA0047273.1 (mitotic spindle checkpoint protein mad1 [Cucumis melo var. makuwa])

HSP 1 Score: 102 bits (253), Expect = 1.11e-21
Identity = 54/166 (32.53%), Postives = 86/166 (51.81%), Query Frame = 0

Query: 13  DWNHLMVVSKSCAQDDWVAIAXALKEKFSLDCVINPIMVDKILSFTRDRILKSSELSVR- 72
           DWN ++V+++    +DW  I   LKE+   +    P   DK L F +D+ L       R 
Sbjct: 163 DWNKIVVLTRRYFHNDWRKIIVKLKEQLDTNLSYQPFHADKALIFIKDKSLVRLLCKNRG 222

Query: 73  WRKTGNFHIKLEKWSPYLHNRKEFIQVYGGCLD---LPLYYWSRSTFEAIGVHFGGLLKI 132
           W   GN  +K E+W+  +H     +  YGG      +PL+ W+ +TF  I    GG +++
Sbjct: 223 WSTVGNVDVKFEEWNHAIHAMPRLMSSYGGWTKFRGIPLHAWNLNTFTQIRNACGGFMEV 282

Query: 133 STKTLNMLEASEAYIKVKPNLCGFIPATVTIKDEKCGSFEVRVFAK 174
           + +T N ++  EA IK+K N   FIPAT+++ D+K   F V+   K
Sbjct: 283 TRETRNKVDLIEAVIKIKYNYTVFIPATISVSDDKGNKFLVQTVVK 328

BLAST of MC02g1298.1 vs. ExPASy TrEMBL
Match: A0A5A7TEP0 (DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00440 PE=4 SV=1)

HSP 1 Score: 108 bits (270), Expect = 1.62e-23
Identity = 63/177 (35.59%), Postives = 96/177 (54.24%), Query Frame = 0

Query: 3   VQKEIEVLDLDWNHLMVVSKSCAQDDWVAIAXALKE----KFSLDCVINPIMVDKILSFT 62
           ++KE    D+DW   +++S+ C  DDW  I   L+E    K S  C + P   DK L F 
Sbjct: 203 IRKEERKNDIDWEKTIILSRRCFHDDWDKIIDRLREQTDKKESCFCYV-PFHADKALLFI 262

Query: 63  RDRIL-KSSELSVRWRKTGNFHIKLEKWSPYLHNRKEFIQVYGGCL---DLPLYYWSRST 122
           +D+ L K    +  W   G F++K EKWS  +H   + I  YGG      +PL+ W+ +T
Sbjct: 263 KDKDLAKLLCKNNGWTTVGPFYVKFEKWSKSVHADTKVIPSYGGWTRFRGIPLHIWNLNT 322

Query: 123 FEAIGVHFGGLLKISTKTLNMLEASEAYIKVKPNLCGFIPATVTIKDEKCGSFEVRV 171
           F  IG   GG +  + +++N +E +EA IKVK N  GF+PA + I DE+   F +++
Sbjct: 323 FIHIGEACGGFIDAAPESVNKIELTEALIKVKENYTGFLPAFIQIHDEEGHVFIIQI 378

BLAST of MC02g1298.1 vs. ExPASy TrEMBL
Match: A0A5D3DLT1 (DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00990 PE=4 SV=1)

HSP 1 Score: 108 bits (270), Expect = 1.72e-23
Identity = 62/175 (35.43%), Postives = 94/175 (53.71%), Query Frame = 0

Query: 3   VQKEIEVLDLDWNHLMVVSKSCAQDDWVAIAXALKEKFSL-DCVIN--PIMVDKILSFTR 62
           ++KE +  D+DW   +++S+ C  DDW  I   LK++    D      P   DK L F +
Sbjct: 209 IRKEEKKNDIDWEKSIILSRRCFHDDWAKIIDRLKDQTDKKDSCFRYIPFHADKALLFIK 268

Query: 63  DRILKSSEL-SVRWRKTGNFHIKLEKWSPYLHNRKEFIQVYGGCL---DLPLYYWSRSTF 122
           D+ L +    ++ W   G F++K EKWS   H   + I  YGG      +PL+ W+ +TF
Sbjct: 269 DKELANLLCKNIGWTTVGPFYVKFEKWSKNAHADTKVIPSYGGWTRFRGIPLHIWNLNTF 328

Query: 123 EAIGVHFGGLLKISTKTLNMLEASEAYIKVKPNLCGFIPATVTIKDEKCGSFEVR 170
             IG   GG +  + +++N LE +EA IKVK N  GF+PA + I DE+   F V+
Sbjct: 329 IQIGEACGGFIDAAPESINKLELTEALIKVKENYTGFLPAFIQIHDEEGQDFIVQ 383

BLAST of MC02g1298.1 vs. ExPASy TrEMBL
Match: A0A5D3BI91 (Putative 3,4-dihydroxy-2-butanone kinase isoform X5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00280 PE=4 SV=1)

HSP 1 Score: 102 bits (254), Expect = 2.31e-22
Identity = 50/107 (46.73%), Postives = 67/107 (62.62%), Query Frame = 0

Query: 61  RILKSSELSVRWRKTGNFHIKLEKWSPYLHNRKEFIQVYGGCL---DLPLYYWSRSTFEA 120
           R+    +   +W+  GNFH+K+E+W    H+     + YGG L   +LPL YW RST E 
Sbjct: 178 RVCLGKDQQGKWQTWGNFHLKIERWDNLKHSWPLVQKGYGGWLKIKNLPLDYWCRSTLEV 237

Query: 121 IGVHFGGLLKISTKTLNMLEASEAYIKVKPNLCGFIPATVTIKDEKC 164
           IG HF GL  I+ +TLN+   SEA I+VK NLCGF+P+T+ I D KC
Sbjct: 238 IGDHFRGLTDIAFETLNLTNVSEARIQVKKNLCGFVPSTIEITDLKC 284

BLAST of MC02g1298.1 vs. ExPASy TrEMBL
Match: A0A5A7TUN4 (Mitotic spindle checkpoint protein mad1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G00660 PE=4 SV=1)

HSP 1 Score: 102 bits (253), Expect = 5.39e-22
Identity = 54/166 (32.53%), Postives = 86/166 (51.81%), Query Frame = 0

Query: 13  DWNHLMVVSKSCAQDDWVAIAXALKEKFSLDCVINPIMVDKILSFTRDRILKSSELSVR- 72
           DWN ++V+++    +DW  I   LKE+   +    P   DK L F +D+ L       R 
Sbjct: 163 DWNKIVVLTRRYFHNDWRKIIVKLKEQLDTNLSYQPFHADKALIFIKDKSLVRLLCKNRG 222

Query: 73  WRKTGNFHIKLEKWSPYLHNRKEFIQVYGGCLD---LPLYYWSRSTFEAIGVHFGGLLKI 132
           W   GN  +K E+W+  +H     +  YGG      +PL+ W+ +TF  I    GG +++
Sbjct: 223 WSTVGNVDVKFEEWNHAIHAMPRLMSSYGGWTKFRGIPLHAWNLNTFTQIRNACGGFMEV 282

Query: 133 STKTLNMLEASEAYIKVKPNLCGFIPATVTIKDEKCGSFEVRVFAK 174
           + +T N ++  EA IK+K N   FIPAT+++ D+K   F V+   K
Sbjct: 283 TRETRNKVDLIEAVIKIKYNYTVFIPATISVSDDKGNKFLVQTVVK 328

BLAST of MC02g1298.1 vs. ExPASy TrEMBL
Match: A0A5A7TRS9 (DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003060 PE=4 SV=1)

HSP 1 Score: 103 bits (256), Expect = 1.18e-21
Identity = 61/175 (34.86%), Postives = 92/175 (52.57%), Query Frame = 0

Query: 3   VQKEIEVLDLDWNHLMVVSKSCAQDDWVAIAXALKEKFSL-DCVIN--PIMVDKILSFTR 62
           ++KE    D+DW   +++S+ C  DDW  I   L+E+    D      P   DK L F +
Sbjct: 180 IRKEERKNDIDWEKTIILSRRCFHDDWDKIIDRLREQTDKKDSCFRYVPFHADKALLFIK 239

Query: 63  DRIL-KSSELSVRWRKTGNFHIKLEKWSPYLHNRKEFIQVYGGCL---DLPLYYWSRSTF 122
           D+ L K    +  W   G F++K EKWS   H   + I  YGG      +PL+ W+ +TF
Sbjct: 240 DKDLAKLLCKNNGWTTVGPFYVKFEKWSKSAHADTKVIPSYGGWTRFRGIPLHIWNLNTF 299

Query: 123 EAIGVHFGGLLKISTKTLNMLEASEAYIKVKPNLCGFIPATVTIKDEKCGSFEVR 170
             IG   GG +  + +++N +E +EA IKVK N  GF+PA + I DE+   F ++
Sbjct: 300 IQIGEACGGFIDAAPESVNKIELTEALIKVKENYTGFLPAFIQIHDEEGHVFIIQ 354