MC01g_new0489.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC01g_new0489.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	FAD-binding Berberine family protein
Location	MC01: 20394348 .. 20395094 (+)
Sequence length	747
RNA-Seq Expression	MC01g_new0489.1
Synteny	MC01g_new0489.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTACTTGTTATTCTGATGAACAAAACTTTCTCCAATGTCTGTCAAGCAACACGCAGTCAAATTTCCCTATCTCCGAAGCTATCTTCACCCCCAACAATTCTTCTTTCTTACCGACTCTTAATTTCTATATCAGAAACCCTCGATTCCAGACCCCCCAAACCCCAAAGCCACTCGTCATTATTGCAGCCAAACACGAATCTCATGTCCAAACAACTGTTATTTGTGCCAAACGCGTTGGGCTACAAATCAGAATCCGTAGCGGCGGCCATGATTACGAAGGCCTCTCCTATGTCTCCCAACAACCCTTCATCGTCCTTGACCTGTTCAATCTCCGAGCCATTAATGTCAATATCTCAAACCAAAGCGCTTGGATTCAAGCAGGGGCCACCATGGGGGAACTTTACTACGCCATTGCTAAGAGGAGTGGAGTCCATGCCTTCCCTGGAGGTGTCTGCCCGACGTTGGGCGCTGGTGGGCATCTTTTAGGAGGTGGATACGGCAATTTAATGAGAAAATTTGGGCTCTCCGTGGACAACATTGTGGATGCACAAATTGTAAATGTACAGGGTCATATTCTAAATCGACAACAAATGGGAGATGATCTGTTCTGGGCTATTAGAGGAGGAGGAGGGAGCTTTGGAGTCATTCTTTCGTGGAAGATTAAGCTAGTTCCAGTCCCTTCAATAGTGACAGTGTTTAATGTTGATAGAACTATAGAGGAGGGAGCAACGAATGTGGTTTGA

mRNA sequence

ATGTCTACTTGTTATTCTGATGAACAAAACTTTCTCCAATGTCTGTCAAGCAACACGCAGTCAAATTTCCCTATCTCCGAAGCTATCTTCACCCCCAACAATTCTTCTTTCTTACCGACTCTTAATTTCTATATCAGAAACCCTCGATTCCAGACCCCCCAAACCCCAAAGCCACTCGTCATTATTGCAGCCAAACACGAATCTCATGTCCAAACAACTGTTATTTGTGCCAAACGCGTTGGGCTACAAATCAGAATCCGTAGCGGCGGCCATGATTACGAAGGCCTCTCCTATGTCTCCCAACAACCCTTCATCGTCCTTGACCTGTTCAATCTCCGAGCCATTAATGTCAATATCTCAAACCAAAGCGCTTGGATTCAAGCAGGGGCCACCATGGGGGAACTTTACTACGCCATTGCTAAGAGGAGTGGAGTCCATGCCTTCCCTGGAGGTGTCTGCCCGACGTTGGGCGCTGGTGGGCATCTTTTAGGAGGTGGATACGGCAATTTAATGAGAAAATTTGGGCTCTCCGTGGACAACATTGTGGATGCACAAATTGTAAATGTACAGGGTCATATTCTAAATCGACAACAAATGGGAGATGATCTGTTCTGGGCTATTAGAGGAGGAGGAGGGAGCTTTGGAGTCATTCTTTCGTGGAAGATTAAGCTAGTTCCAGTCCCTTCAATAGTGACAGTGTTTAATGTTGATAGAACTATAGAGGAGGGAGCAACGAATGTGGTTTGA

Coding sequence (CDS)

ATGTCTACTTGTTATTCTGATGAACAAAACTTTCTCCAATGTCTGTCAAGCAACACGCAGTCAAATTTCCCTATCTCCGAAGCTATCTTCACCCCCAACAATTCTTCTTTCTTACCGACTCTTAATTTCTATATCAGAAACCCTCGATTCCAGACCCCCCAAACCCCAAAGCCACTCGTCATTATTGCAGCCAAACACGAATCTCATGTCCAAACAACTGTTATTTGTGCCAAACGCGTTGGGCTACAAATCAGAATCCGTAGCGGCGGCCATGATTACGAAGGCCTCTCCTATGTCTCCCAACAACCCTTCATCGTCCTTGACCTGTTCAATCTCCGAGCCATTAATGTCAATATCTCAAACCAAAGCGCTTGGATTCAAGCAGGGGCCACCATGGGGGAACTTTACTACGCCATTGCTAAGAGGAGTGGAGTCCATGCCTTCCCTGGAGGTGTCTGCCCGACGTTGGGCGCTGGTGGGCATCTTTTAGGAGGTGGATACGGCAATTTAATGAGAAAATTTGGGCTCTCCGTGGACAACATTGTGGATGCACAAATTGTAAATGTACAGGGTCATATTCTAAATCGACAACAAATGGGAGATGATCTGTTCTGGGCTATTAGAGGAGGAGGAGGGAGCTTTGGAGTCATTCTTTCGTGGAAGATTAAGCTAGTTCCAGTCCCTTCAATAGTGACAGTGTTTAATGTTGATAGAACTATAGAGGAGGGAGCAACGAATGTGGTTTGA

Protein sequence

MSTCYSDEQNFLQCLSSNTQSNFPISEAIFTPNNSSFLPTLNFYIRNPRFQTPQTPKPLVIIAAKHESHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNISNQSAWIQAGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDNIVDAQIVNVQGHILNRQQMGDDLFWAIRGGGGSFGVILSWKIKLVPVPSIVTVFNVDRTIEEGATNVV

Homology

[Close]

BLAST of MC01g_new0489.1 vs. ExPASy Swiss-Prot
Match: Q9FZC5 (Berberine bridge enzyme-like 4 OS=Arabidopsis thaliana OX=3702 GN=FOX2 PE=2 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 8.2e-81
Identity = 146/241 (60.58%), Postives = 188/241 (78.01%), Query Frame = 0


Query: 10  NFLQCLSSNTQSNFPISEAI-FTPNNSSFLPTLNFYIRNPRFQTPQTPKPLVIIAAKHES 69
           +F+ CL   T    PI++AI F  N ++FL +   Y +N RF TP   K L I+AAKH S
Sbjct: 28  DFIGCLRYRTSPENPITDAISFADNTTTFLSSYVSYTKNKRFSTPNYRKLLAIVAAKHVS 87

Query: 70  HVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNISNQSAWIQA 129
           HVQ TV+CAK  G+Q+RIRSGGHDYEGLSY+S  PF++LD++NLR+I V++S++ AWIQA
Sbjct: 88  HVQATVVCAKSNGIQLRIRSGGHDYEGLSYMSSVPFVILDMYNLRSITVDVSSKKAWIQA 147

Query: 130 GATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDNIVDAQIVN 189
           GAT+GELY  +   S   AFP GVC T+GAGGH+ GGGYGNLMRK+G++VD+++DAQI++
Sbjct: 148 GATLGELYTNVNDVSQTLAFPAGVCATVGAGGHISGGGYGNLMRKYGITVDHVIDAQIID 207

Query: 190 VQGHILNRQQMGDDLFWAIR-GGGGSFGVILSWKIKLVPVPSIVTVFNVDRTIEEGATNV 249
           V G +LNR  MG+DLFWAIR GGGGSFGVILSWKI LV VP IVTVF V++T+E+G T+V
Sbjct: 208 VNGKLLNRATMGEDLFWAIRGGGGGSFGVILSWKINLVDVPKIVTVFKVNKTLEQGGTDV 267

[Close]

BLAST of MC01g_new0489.1 vs. ExPASy Swiss-Prot
Match: Q9FZC7 (Berberine bridge enzyme-like 6 OS=Arabidopsis thaliana OX=3702 GN=FOX4 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 2.4e-80
Identity = 141/239 (59.00%), Postives = 186/239 (77.82%), Query Frame = 0


Query: 11  FLQCLSSNTQSNFPISEAI-FTPNNSSFLPTLNFYIRNPRFQTPQTPKPLVIIAAKHESH 70
           F++CL   T    PI++ I    N+++FL +   Y +N RF +P   K L IIAAKH SH
Sbjct: 53  FIECLRDRTTPENPITDVISIADNSTTFLSSYVSYTKNKRFSSPNFKKLLAIIAAKHVSH 112

Query: 71  VQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNISNQSAWIQAG 130
           VQ TV+CAK  G+Q+RIRSGGHD EG SY+S  PF++LD+ NLR+I+VN+S ++AW+QAG
Sbjct: 113 VQATVVCAKSNGIQLRIRSGGHDNEGFSYMSSVPFVILDMHNLRSIDVNLSRKNAWVQAG 172

Query: 131 ATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDNIVDAQIVNV 190
           AT+GELY  I + S   AFP GVCPT+GAGGH+ GGG+GNLMRKFG++VD+++DAQI++V
Sbjct: 173 ATLGELYVKINEASQTLAFPAGVCPTVGAGGHISGGGFGNLMRKFGITVDHVIDAQIIDV 232

Query: 191 QGHILNRQQMGDDLFWAIRGGGGSFGVILSWKIKLVPVPSIVTVFNVDRTIEEGATNVV 249
            G +LNR  MG+DLFWAIRGGG SFGVILSWKI LV VP I+TVF V++T+E+G T+++
Sbjct: 233 NGKLLNRAAMGEDLFWAIRGGGSSFGVILSWKINLVEVPKILTVFKVNKTLEQGGTDIL 291

[Close]

BLAST of MC01g_new0489.1 vs. ExPASy Swiss-Prot
Match: Q9FZC8 (Berberine bridge enzyme-like 7 OS=Arabidopsis thaliana OX=3702 GN=FOX5 PE=3 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 2.4e-80
Identity = 143/244 (58.61%), Postives = 188/244 (77.05%), Query Frame = 0


Query: 7   DEQNFLQCLSSNTQSNFPISEAI-FTPNNSSFLPTLNFYIRNPRFQTPQTPKPLVIIAAK 66
           + +NF++CL   T S  PI+++I    N ++FL +   Y +N R+ +P   K L I+AAK
Sbjct: 25  NSENFIECLRYRTSSENPITDSISIADNTTTFLSSYLSYTKNKRYSSPNFKKLLAIVAAK 84

Query: 67  HESHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNISNQSAW 126
           H SHVQ TV+CAK  G+Q+RIRSGGHD EGLSY S  PF++LD+FNLR+I VN+ ++ AW
Sbjct: 85  HVSHVQATVVCAKTNGIQLRIRSGGHDLEGLSYRSSVPFVILDMFNLRSITVNVLSKKAW 144

Query: 127 IQAGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDNIVDAQ 186
           +QAGAT+GELY  I + S   AFP GVCPT+G GGH+ GGGYGNLMRKFG++VD++ DAQ
Sbjct: 145 VQAGATLGELYVKINEASQTLAFPAGVCPTVGVGGHISGGGYGNLMRKFGITVDHVSDAQ 204

Query: 187 IVNVQGHILNRQQMGDDLFWAIRGGGG-SFGVILSWKIKLVPVPSIVTVFNVDRTIEEGA 246
           +++V G +LNR  MG+DLFWAIRGGGG SFGVILSWKI LV VP I+TVF V++T+E+G 
Sbjct: 205 LIDVNGKLLNRASMGEDLFWAIRGGGGASFGVILSWKINLVKVPKILTVFKVNKTLEQGG 264

Query: 247 TNVV 249
           T+V+
Sbjct: 265 TDVL 268

[Close]

BLAST of MC01g_new0489.1 vs. ExPASy Swiss-Prot
Match: Q9SVG7 (Berberine bridge enzyme-like 17 OS=Arabidopsis thaliana OX=3702 GN=At4g20800 PE=2 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 4.5e-79
Identity = 144/241 (59.75%), Postives = 182/241 (75.52%), Query Frame = 0


Query: 9   QNFLQCLSSNTQSNFPISEAIFTPNNSSFLPTLNFYIRNPRFQTPQTPKPLVIIAAKHES 68
           +NFL+CL + T    PI+EAI+T  NS+F  +   Y  N R   P   K + I+AAKHES
Sbjct: 27  ENFLRCLRNRTNPKNPIAEAIYTHENSTFASSYVSYTNNKRCLNPNDTKLIAIVAAKHES 86

Query: 69  HVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNISNQSAWIQA 128
           HVQ TV+CAK  G+QIRIRSGGHDYEGLS+ S  PF++LD+ +LR+I +++  + AW+ A
Sbjct: 87  HVQATVVCAKSNGIQIRIRSGGHDYEGLSFTSSVPFVILDMHDLRSITIDVFRKQAWVDA 146

Query: 129 GATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDNIVDAQIVN 188
           GATMGELY  IA  S   AF GGVCPTLGAGGH+ GGGYGNL+RK+G+SVD++VDA+IV+
Sbjct: 147 GATMGELYTKIAAASKTLAFAGGVCPTLGAGGHISGGGYGNLIRKYGISVDHVVDARIVD 206

Query: 189 VQGHILNRQQMGDDLFWAIRGGGG-SFGVILSWKIKLVPVPSIVTVFNVDRTIEEGATNV 248
           V G+IL    +G DL WAIRGGGG SFGVILSWKI LV VP  VTVF V++T+E+G T+V
Sbjct: 207 VNGNILTGATLGRDLLWAIRGGGGASFGVILSWKINLVDVPKTVTVFKVNKTLEQGVTDV 266

[Close]

BLAST of MC01g_new0489.1 vs. ExPASy Swiss-Prot
Match: Q9FZC6 (Berberine bridge enzyme-like 5 OS=Arabidopsis thaliana OX=3702 GN=FOX3 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.3e-78
Identity = 141/244 (57.79%), Postives = 186/244 (76.23%), Query Frame = 0


Query: 7   DEQNFLQCLSSNTQSNFPISEAIFTP-NNSSFLPTLNFYIRNPRFQTPQTPKPLVIIAAK 66
           D   F  CL + T    PI++AIFT  N ++FL +   Y +N R+ +    K + I+AAK
Sbjct: 25  DSGIFTGCLRNRTSLENPITDAIFTSRNTTTFLSSYVSYTKNKRYSSLNYQKLVAIVAAK 84

Query: 67  HESHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNISNQSAW 126
           H SHVQ TV+CAK  G+Q+RIRSGGHDYEGLSY S  PF++LD++NLR+I V++S++ AW
Sbjct: 85  HVSHVQATVVCAKANGIQLRIRSGGHDYEGLSYTSSVPFVILDMYNLRSITVDVSSKKAW 144

Query: 127 IQAGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDNIVDAQ 186
           +QAGAT+GELY  I + S   AFP GVCPT+G GGH+ GGG+GNLMRKFG++VD+++DAQ
Sbjct: 145 VQAGATLGELYTKINEASQTLAFPAGVCPTVGVGGHITGGGFGNLMRKFGITVDHVIDAQ 204

Query: 187 IVNVQGHILNRQQMGDDLFWAIRGGGG-SFGVILSWKIKLVPVPSIVTVFNVDRTIEEGA 246
           ++ V G +L+R  MG+DLFWAIRGGGG SFGVILSWKI LV VP I+TVF V +T+E+G 
Sbjct: 205 LIGVNGKLLDRATMGEDLFWAIRGGGGASFGVILSWKINLVEVPKILTVFKVSKTLEQGG 264

Query: 247 TNVV 249
           T+V+
Sbjct: 265 TDVL 268

[Close]

BLAST of MC01g_new0489.1 vs. NCBI nr
Match: XP_022146700.1 (LOW QUALITY PROTEIN: berberine bridge enzyme-like 14 [Momordica charantia])

HSP 1 Score: 499 bits (1285), Expect = 6.44e-174
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   MSTCYSDEQNFLQCLSSNTQSNFPISEAIFTPNNSSFLPTLNFYIRNPRFQTPQTPKPLV 60
           MSTCYSDEQNFLQCLSSNTQSNFPISEAIFTPNNSSFLPTLNFYIRNPRFQTPQTPKPLV
Sbjct: 22  MSTCYSDEQNFLQCLSSNTQSNFPISEAIFTPNNSSFLPTLNFYIRNPRFQTPQTPKPLV 81

Query: 61  IIAAKHESHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNIS 120
           IIAAKHESHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNIS
Sbjct: 82  IIAAKHESHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNIS 141

Query: 121 NQSAWIQAGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDN 180
           NQSAWIQAGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDN
Sbjct: 142 NQSAWIQAGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDN 201

Query: 181 IVDAQIVNVQGHILNRQQMGDDLFWAIRGGGGSFGVILSWKIKLVPVPSIVTVFNVDRTI 240
           IVDAQIVNVQGHILNRQQMGDDLFWAIRGGGGSFGVILSWKIKLVPVPSIVTVFNVDRTI
Sbjct: 202 IVDAQIVNVQGHILNRQQMGDDLFWAIRGGGGSFGVILSWKIKLVPVPSIVTVFNVDRTI 261

Query: 241 EEGATNVV 248
           EEGATNVV
Sbjct: 262 EEGATNVV 269

[Close]

BLAST of MC01g_new0489.1 vs. NCBI nr
Match: XP_022159193.1 (berberine bridge enzyme-like 14 [Momordica charantia])

HSP 1 Score: 446 bits (1148), Expect = 4.39e-153
Identity = 222/249 (89.16%), Postives = 233/249 (93.57%), Query Frame = 0


Query: 1   MSTCYSDEQNFLQCLSSNTQSNFPISEAIFTPNNSSFLPTLNFYIRNPRFQTPQTPKPLV 60
           +STC   E  FLQCLSSN Q NFPISEAIFTPNNSSFLPTLNFYIRNPRFQ PQTPKPL 
Sbjct: 24  VSTCCFAEWIFLQCLSSNGQPNFPISEAIFTPNNSSFLPTLNFYIRNPRFQNPQTPKPLA 83

Query: 61  IIAAKHESHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNIS 120
           IIAAKHESHVQ T+ICAKRVGL IRIRSGGHDYEGLSY+SQQPFIVLDLFNLRAINVNI+
Sbjct: 84  IIAAKHESHVQATIICAKRVGLHIRIRSGGHDYEGLSYISQQPFIVLDLFNLRAINVNIA 143

Query: 121 NQSAWIQAGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDN 180
           +QSAW+QAGATMGELYYAIAKRSGVHAFPGGVCPT+GAGGH+ GGGYGNLMRKFGLSVDN
Sbjct: 144 DQSAWVQAGATMGELYYAIAKRSGVHAFPGGVCPTMGAGGHISGGGYGNLMRKFGLSVDN 203

Query: 181 IVDAQIVNVQGHILNRQQMGDDLFWAIRGGGG-SFGVILSWKIKLVPVPSIVTVFNVDRT 240
           IVDAQIVNV GHILNR+QMG+DLFWAIRGGGG SFGVILSWKIKLVPVPSIVTVFNV RT
Sbjct: 204 IVDAQIVNVHGHILNRKQMGEDLFWAIRGGGGGSFGVILSWKIKLVPVPSIVTVFNVGRT 263

Query: 241 IEEGATNVV 248
           IEEGA +VV
Sbjct: 264 IEEGAADVV 272

[Close]

BLAST of MC01g_new0489.1 vs. NCBI nr
Match: XP_038883273.1 (berberine bridge enzyme-like 14 [Benincasa hispida])

HSP 1 Score: 411 bits (1057), Expect = 2.79e-139
Identity = 202/242 (83.47%), Postives = 225/242 (92.98%), Query Frame = 0


Query: 8   EQNFLQCLSSNTQSNFPISEAIFTPNNSSFLPTLNFYIRNPRFQTPQTPKPLVIIAAKHE 67
           +Q+FLQCL SN+Q  FPIS+AIFTPNNSSFLP LN YIRN RFQTP TPKPLVIIAAKH 
Sbjct: 33  QQSFLQCLISNSQPEFPISDAIFTPNNSSFLPVLNSYIRNLRFQTPTTPKPLVIIAAKHR 92

Query: 68  SHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNISNQSAWIQ 127
           SHVQ+TVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFI+LDLFNLRAINV+I  ++AW++
Sbjct: 93  SHVQSTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIILDLFNLRAINVDIPTETAWVE 152

Query: 128 AGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDNIVDAQIV 187
           AGATMGELYYAIAK+S VHAFPGG+CPTLGAGGH+ GGGYGN+MRKFGLSVDNI+DA+IV
Sbjct: 153 AGATMGELYYAIAKQSKVHAFPGGICPTLGAGGHISGGGYGNMMRKFGLSVDNILDAEIV 212

Query: 188 NVQGHILNRQQMGDDLFWAIRGGGG-SFGVILSWKIKLVPVPSIVTVFNVDRTIEEGATN 247
           NV+G ILNRQQMG+DLFWAIRGGGG SFGVILSWKIKLV VPS VTVF+V+R IE+GAT+
Sbjct: 213 NVEGKILNRQQMGEDLFWAIRGGGGGSFGVILSWKIKLVQVPSTVTVFDVERKIEDGATD 272

[Close]

BLAST of MC01g_new0489.1 vs. NCBI nr
Match: XP_008441073.1 (PREDICTED: flavin-dependent oxidoreductase FOX2-like [Cucumis melo])

HSP 1 Score: 400 bits (1029), Expect = 4.40e-135
Identity = 197/242 (81.40%), Postives = 222/242 (91.74%), Query Frame = 0


Query: 8   EQNFLQCLSSNTQSNFPISEAIFTPNNSSFLPTLNFYIRNPRFQTPQTPKPLVIIAAKHE 67
           +Q+FLQCL+SN+   FPIS+AIFTP+++SFLP LN YIRN  FQTP TPKPLVI+ AKH 
Sbjct: 30  QQSFLQCLASNSHPKFPISDAIFTPDDASFLPVLNSYIRNRIFQTPTTPKPLVIVTAKHP 89

Query: 68  SHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNISNQSAWIQ 127
           SHVQ+TV+CAKRV L+IRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINV+I  ++AW++
Sbjct: 90  SHVQSTVVCAKRVALEIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVDIPTETAWVE 149

Query: 128 AGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDNIVDAQIV 187
           AGATMGELYYAIAK+S VHAFPGGVCPTLGAGGH+ GGGYGNLMRKFGLSVDNI+DAQIV
Sbjct: 150 AGATMGELYYAIAKQSKVHAFPGGVCPTLGAGGHISGGGYGNLMRKFGLSVDNILDAQIV 209

Query: 188 NVQGHILNRQQMGDDLFWAIRGGGG-SFGVILSWKIKLVPVPSIVTVFNVDRTIEEGATN 247
           NV+G ILNRQQMG+DLFWAIRGGGG SFGVILSWKIKLV VPS VTVF+V+R IEEGAT+
Sbjct: 210 NVEGKILNRQQMGEDLFWAIRGGGGGSFGVILSWKIKLVQVPSTVTVFDVERKIEEGATD 269

[Close]

BLAST of MC01g_new0489.1 vs. NCBI nr
Match: XP_008439524.1 (PREDICTED: flavin-dependent oxidoreductase FOX2-like [Cucumis melo])

HSP 1 Score: 393 bits (1009), Expect = 4.54e-132
Identity = 190/242 (78.51%), Postives = 218/242 (90.08%), Query Frame = 0


Query: 8   EQNFLQCLSSNTQSNFPISEAIFTPNNSSFLPTLNFYIRNPRFQTPQTPKPLVIIAAKHE 67
           +Q+FLQCL+S +Q  FPIS+AIFTPNNSSFLP LN YIRN RFQTP TPKPL+I+ AKH 
Sbjct: 29  QQSFLQCLTSTSQPQFPISDAIFTPNNSSFLPVLNSYIRNLRFQTPTTPKPLLIVTAKHY 88

Query: 68  SHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNISNQSAWIQ 127
           SHVQ+TV+CAKRVGLQIRIRSGGHD+EGLSYVSQQPFI+LDLFNLRAINV+I  Q+AW++
Sbjct: 89  SHVQSTVVCAKRVGLQIRIRSGGHDFEGLSYVSQQPFIILDLFNLRAINVDIPTQTAWVE 148

Query: 128 AGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDNIVDAQIV 187
           +GAT+GELYYAIAK+S +HAFPGGVCPT+GAGGH  GGGYGNL+RK+GL+VDNI+DAQIV
Sbjct: 149 SGATLGELYYAIAKKSNLHAFPGGVCPTVGAGGHFSGGGYGNLLRKYGLTVDNILDAQIV 208

Query: 188 NVQGHILNRQQMGDDLFWAIRGGGG-SFGVILSWKIKLVPVPSIVTVFNVDRTIEEGATN 247
              G ILNRQ MG+DLFWAIRGGGG SFGVILSWKI LV VPS VTVF+VDR IE+GAT+
Sbjct: 209 TADGKILNRQTMGEDLFWAIRGGGGGSFGVILSWKISLVQVPSTVTVFDVDRKIEDGATD 268

[Close]

BLAST of MC01g_new0489.1 vs. ExPASy TrEMBL
Match: A0A6J1CY03 (LOW QUALITY PROTEIN: berberine bridge enzyme-like 14 OS=Momordica charantia OX=3673 GN=LOC111015841 PE=3 SV=1)

HSP 1 Score: 499 bits (1285), Expect = 3.12e-174
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   MSTCYSDEQNFLQCLSSNTQSNFPISEAIFTPNNSSFLPTLNFYIRNPRFQTPQTPKPLV 60
           MSTCYSDEQNFLQCLSSNTQSNFPISEAIFTPNNSSFLPTLNFYIRNPRFQTPQTPKPLV
Sbjct: 22  MSTCYSDEQNFLQCLSSNTQSNFPISEAIFTPNNSSFLPTLNFYIRNPRFQTPQTPKPLV 81

Query: 61  IIAAKHESHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNIS 120
           IIAAKHESHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNIS
Sbjct: 82  IIAAKHESHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNIS 141

Query: 121 NQSAWIQAGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDN 180
           NQSAWIQAGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDN
Sbjct: 142 NQSAWIQAGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDN 201

Query: 181 IVDAQIVNVQGHILNRQQMGDDLFWAIRGGGGSFGVILSWKIKLVPVPSIVTVFNVDRTI 240
           IVDAQIVNVQGHILNRQQMGDDLFWAIRGGGGSFGVILSWKIKLVPVPSIVTVFNVDRTI
Sbjct: 202 IVDAQIVNVQGHILNRQQMGDDLFWAIRGGGGSFGVILSWKIKLVPVPSIVTVFNVDRTI 261

Query: 241 EEGATNVV 248
           EEGATNVV
Sbjct: 262 EEGATNVV 269

[Close]

BLAST of MC01g_new0489.1 vs. ExPASy TrEMBL
Match: A0A6J1DY47 (berberine bridge enzyme-like 14 OS=Momordica charantia OX=3673 GN=LOC111025613 PE=3 SV=1)

HSP 1 Score: 446 bits (1148), Expect = 2.13e-153
Identity = 222/249 (89.16%), Postives = 233/249 (93.57%), Query Frame = 0


Query: 1   MSTCYSDEQNFLQCLSSNTQSNFPISEAIFTPNNSSFLPTLNFYIRNPRFQTPQTPKPLV 60
           +STC   E  FLQCLSSN Q NFPISEAIFTPNNSSFLPTLNFYIRNPRFQ PQTPKPL 
Sbjct: 24  VSTCCFAEWIFLQCLSSNGQPNFPISEAIFTPNNSSFLPTLNFYIRNPRFQNPQTPKPLA 83

Query: 61  IIAAKHESHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNIS 120
           IIAAKHESHVQ T+ICAKRVGL IRIRSGGHDYEGLSY+SQQPFIVLDLFNLRAINVNI+
Sbjct: 84  IIAAKHESHVQATIICAKRVGLHIRIRSGGHDYEGLSYISQQPFIVLDLFNLRAINVNIA 143

Query: 121 NQSAWIQAGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDN 180
           +QSAW+QAGATMGELYYAIAKRSGVHAFPGGVCPT+GAGGH+ GGGYGNLMRKFGLSVDN
Sbjct: 144 DQSAWVQAGATMGELYYAIAKRSGVHAFPGGVCPTMGAGGHISGGGYGNLMRKFGLSVDN 203

Query: 181 IVDAQIVNVQGHILNRQQMGDDLFWAIRGGGG-SFGVILSWKIKLVPVPSIVTVFNVDRT 240
           IVDAQIVNV GHILNR+QMG+DLFWAIRGGGG SFGVILSWKIKLVPVPSIVTVFNV RT
Sbjct: 204 IVDAQIVNVHGHILNRKQMGEDLFWAIRGGGGGSFGVILSWKIKLVPVPSIVTVFNVGRT 263

Query: 241 IEEGATNVV 248
           IEEGA +VV
Sbjct: 264 IEEGAADVV 272

[Close]

BLAST of MC01g_new0489.1 vs. ExPASy TrEMBL
Match: A0A1S3B2M2 (flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo OX=3656 GN=LOC103485297 PE=3 SV=1)

HSP 1 Score: 400 bits (1029), Expect = 2.13e-135
Identity = 197/242 (81.40%), Postives = 222/242 (91.74%), Query Frame = 0


Query: 8   EQNFLQCLSSNTQSNFPISEAIFTPNNSSFLPTLNFYIRNPRFQTPQTPKPLVIIAAKHE 67
           +Q+FLQCL+SN+   FPIS+AIFTP+++SFLP LN YIRN  FQTP TPKPLVI+ AKH 
Sbjct: 30  QQSFLQCLASNSHPKFPISDAIFTPDDASFLPVLNSYIRNRIFQTPTTPKPLVIVTAKHP 89

Query: 68  SHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNISNQSAWIQ 127
           SHVQ+TV+CAKRV L+IRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINV+I  ++AW++
Sbjct: 90  SHVQSTVVCAKRVALEIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVDIPTETAWVE 149

Query: 128 AGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDNIVDAQIV 187
           AGATMGELYYAIAK+S VHAFPGGVCPTLGAGGH+ GGGYGNLMRKFGLSVDNI+DAQIV
Sbjct: 150 AGATMGELYYAIAKQSKVHAFPGGVCPTLGAGGHISGGGYGNLMRKFGLSVDNILDAQIV 209

Query: 188 NVQGHILNRQQMGDDLFWAIRGGGG-SFGVILSWKIKLVPVPSIVTVFNVDRTIEEGATN 247
           NV+G ILNRQQMG+DLFWAIRGGGG SFGVILSWKIKLV VPS VTVF+V+R IEEGAT+
Sbjct: 210 NVEGKILNRQQMGEDLFWAIRGGGGGSFGVILSWKIKLVQVPSTVTVFDVERKIEEGATD 269

[Close]

BLAST of MC01g_new0489.1 vs. ExPASy TrEMBL
Match: A0A1S3AYX7 (flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo OX=3656 GN=LOC103484293 PE=3 SV=1)

HSP 1 Score: 393 bits (1009), Expect = 2.20e-132
Identity = 190/242 (78.51%), Postives = 218/242 (90.08%), Query Frame = 0


Query: 8   EQNFLQCLSSNTQSNFPISEAIFTPNNSSFLPTLNFYIRNPRFQTPQTPKPLVIIAAKHE 67
           +Q+FLQCL+S +Q  FPIS+AIFTPNNSSFLP LN YIRN RFQTP TPKPL+I+ AKH 
Sbjct: 29  QQSFLQCLTSTSQPQFPISDAIFTPNNSSFLPVLNSYIRNLRFQTPTTPKPLLIVTAKHY 88

Query: 68  SHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNISNQSAWIQ 127
           SHVQ+TV+CAKRVGLQIRIRSGGHD+EGLSYVSQQPFI+LDLFNLRAINV+I  Q+AW++
Sbjct: 89  SHVQSTVVCAKRVGLQIRIRSGGHDFEGLSYVSQQPFIILDLFNLRAINVDIPTQTAWVE 148

Query: 128 AGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDNIVDAQIV 187
           +GAT+GELYYAIAK+S +HAFPGGVCPT+GAGGH  GGGYGNL+RK+GL+VDNI+DAQIV
Sbjct: 149 SGATLGELYYAIAKKSNLHAFPGGVCPTVGAGGHFSGGGYGNLLRKYGLTVDNILDAQIV 208

Query: 188 NVQGHILNRQQMGDDLFWAIRGGGG-SFGVILSWKIKLVPVPSIVTVFNVDRTIEEGATN 247
              G ILNRQ MG+DLFWAIRGGGG SFGVILSWKI LV VPS VTVF+VDR IE+GAT+
Sbjct: 209 TADGKILNRQTMGEDLFWAIRGGGGGSFGVILSWKISLVQVPSTVTVFDVDRKIEDGATD 268

[Close]

BLAST of MC01g_new0489.1 vs. ExPASy TrEMBL
Match: A0A5A7UD95 (Flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009810 PE=3 SV=1)

HSP 1 Score: 392 bits (1007), Expect = 4.41e-132
Identity = 190/242 (78.51%), Postives = 218/242 (90.08%), Query Frame = 0


Query: 8   EQNFLQCLSSNTQSNFPISEAIFTPNNSSFLPTLNFYIRNPRFQTPQTPKPLVIIAAKHE 67
           +Q+FLQCL+S +Q  FPIS+AIFTPNNSSFLP LN YIRN RFQTP TPKPL+I+ AKH 
Sbjct: 29  QQSFLQCLTSTSQPQFPISDAIFTPNNSSFLPVLNSYIRNLRFQTPTTPKPLLIVTAKHY 88

Query: 68  SHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNISNQSAWIQ 127
           SHVQ+TV+CAKRVGLQIRIRSGGHD+EGLSYVSQQPFI+LDLFNLRAINV+I  Q+AW++
Sbjct: 89  SHVQSTVVCAKRVGLQIRIRSGGHDFEGLSYVSQQPFIILDLFNLRAINVDIPTQTAWVE 148

Query: 128 AGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDNIVDAQIV 187
           +GAT+GELYYAIAK+S +HAFPGGVCPT+GAGGH  GGGYGNL+RK+GL+VDNI+DAQIV
Sbjct: 149 SGATLGELYYAIAKKSKLHAFPGGVCPTVGAGGHFSGGGYGNLLRKYGLTVDNILDAQIV 208

Query: 188 NVQGHILNRQQMGDDLFWAIRGGGG-SFGVILSWKIKLVPVPSIVTVFNVDRTIEEGATN 247
              G ILNRQ MG+DLFWAIRGGGG SFGVILSWKI LV VPS VTVF+VDR IE+GAT+
Sbjct: 209 TADGKILNRQTMGEDLFWAIRGGGGGSFGVILSWKISLVQVPSTVTVFDVDRKIEDGATD 268

[Close]

BLAST of MC01g_new0489.1 vs. TAIR 10
Match: AT1G26390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 301.6 bits (771), Expect = 5.8e-82
Identity = 146/241 (60.58%), Postives = 188/241 (78.01%), Query Frame = 0


Query: 10  NFLQCLSSNTQSNFPISEAI-FTPNNSSFLPTLNFYIRNPRFQTPQTPKPLVIIAAKHES 69
           +F+ CL   T    PI++AI F  N ++FL +   Y +N RF TP   K L I+AAKH S
Sbjct: 28  DFIGCLRYRTSPENPITDAISFADNTTTFLSSYVSYTKNKRFSTPNYRKLLAIVAAKHVS 87

Query: 70  HVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNISNQSAWIQA 129
           HVQ TV+CAK  G+Q+RIRSGGHDYEGLSY+S  PF++LD++NLR+I V++S++ AWIQA
Sbjct: 88  HVQATVVCAKSNGIQLRIRSGGHDYEGLSYMSSVPFVILDMYNLRSITVDVSSKKAWIQA 147

Query: 130 GATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDNIVDAQIVN 189
           GAT+GELY  +   S   AFP GVC T+GAGGH+ GGGYGNLMRK+G++VD+++DAQI++
Sbjct: 148 GATLGELYTNVNDVSQTLAFPAGVCATVGAGGHISGGGYGNLMRKYGITVDHVIDAQIID 207

Query: 190 VQGHILNRQQMGDDLFWAIR-GGGGSFGVILSWKIKLVPVPSIVTVFNVDRTIEEGATNV 249
           V G +LNR  MG+DLFWAIR GGGGSFGVILSWKI LV VP IVTVF V++T+E+G T+V
Sbjct: 208 VNGKLLNRATMGEDLFWAIRGGGGGSFGVILSWKINLVDVPKIVTVFKVNKTLEQGGTDV 267

[Close]

BLAST of MC01g_new0489.1 vs. TAIR 10
Match: AT1G26420.1 (FAD-binding Berberine family protein )

HSP 1 Score: 300.1 bits (767), Expect = 1.7e-81
Identity = 143/244 (58.61%), Postives = 188/244 (77.05%), Query Frame = 0


Query: 7   DEQNFLQCLSSNTQSNFPISEAI-FTPNNSSFLPTLNFYIRNPRFQTPQTPKPLVIIAAK 66
           + +NF++CL   T S  PI+++I    N ++FL +   Y +N R+ +P   K L I+AAK
Sbjct: 25  NSENFIECLRYRTSSENPITDSISIADNTTTFLSSYLSYTKNKRYSSPNFKKLLAIVAAK 84

Query: 67  HESHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNISNQSAW 126
           H SHVQ TV+CAK  G+Q+RIRSGGHD EGLSY S  PF++LD+FNLR+I VN+ ++ AW
Sbjct: 85  HVSHVQATVVCAKTNGIQLRIRSGGHDLEGLSYRSSVPFVILDMFNLRSITVNVLSKKAW 144

Query: 127 IQAGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDNIVDAQ 186
           +QAGAT+GELY  I + S   AFP GVCPT+G GGH+ GGGYGNLMRKFG++VD++ DAQ
Sbjct: 145 VQAGATLGELYVKINEASQTLAFPAGVCPTVGVGGHISGGGYGNLMRKFGITVDHVSDAQ 204

Query: 187 IVNVQGHILNRQQMGDDLFWAIRGGGG-SFGVILSWKIKLVPVPSIVTVFNVDRTIEEGA 246
           +++V G +LNR  MG+DLFWAIRGGGG SFGVILSWKI LV VP I+TVF V++T+E+G 
Sbjct: 205 LIDVNGKLLNRASMGEDLFWAIRGGGGASFGVILSWKINLVKVPKILTVFKVNKTLEQGG 264

Query: 247 TNVV 249
           T+V+
Sbjct: 265 TDVL 268

[Close]

BLAST of MC01g_new0489.1 vs. TAIR 10
Match: AT1G26410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 300.1 bits (767), Expect = 1.7e-81
Identity = 141/239 (59.00%), Postives = 186/239 (77.82%), Query Frame = 0


Query: 11  FLQCLSSNTQSNFPISEAI-FTPNNSSFLPTLNFYIRNPRFQTPQTPKPLVIIAAKHESH 70
           F++CL   T    PI++ I    N+++FL +   Y +N RF +P   K L IIAAKH SH
Sbjct: 53  FIECLRDRTTPENPITDVISIADNSTTFLSSYVSYTKNKRFSSPNFKKLLAIIAAKHVSH 112

Query: 71  VQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNISNQSAWIQAG 130
           VQ TV+CAK  G+Q+RIRSGGHD EG SY+S  PF++LD+ NLR+I+VN+S ++AW+QAG
Sbjct: 113 VQATVVCAKSNGIQLRIRSGGHDNEGFSYMSSVPFVILDMHNLRSIDVNLSRKNAWVQAG 172

Query: 131 ATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDNIVDAQIVNV 190
           AT+GELY  I + S   AFP GVCPT+GAGGH+ GGG+GNLMRKFG++VD+++DAQI++V
Sbjct: 173 ATLGELYVKINEASQTLAFPAGVCPTVGAGGHISGGGFGNLMRKFGITVDHVIDAQIIDV 232

Query: 191 QGHILNRQQMGDDLFWAIRGGGGSFGVILSWKIKLVPVPSIVTVFNVDRTIEEGATNVV 249
            G +LNR  MG+DLFWAIRGGG SFGVILSWKI LV VP I+TVF V++T+E+G T+++
Sbjct: 233 NGKLLNRAAMGEDLFWAIRGGGSSFGVILSWKINLVEVPKILTVFKVNKTLEQGGTDIL 291

[Close]

BLAST of MC01g_new0489.1 vs. TAIR 10
Match: AT4G20800.1 (FAD-binding Berberine family protein )

HSP 1 Score: 295.8 bits (756), Expect = 3.2e-80
Identity = 144/241 (59.75%), Postives = 182/241 (75.52%), Query Frame = 0


Query: 9   QNFLQCLSSNTQSNFPISEAIFTPNNSSFLPTLNFYIRNPRFQTPQTPKPLVIIAAKHES 68
           +NFL+CL + T    PI+EAI+T  NS+F  +   Y  N R   P   K + I+AAKHES
Sbjct: 27  ENFLRCLRNRTNPKNPIAEAIYTHENSTFASSYVSYTNNKRCLNPNDTKLIAIVAAKHES 86

Query: 69  HVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNISNQSAWIQA 128
           HVQ TV+CAK  G+QIRIRSGGHDYEGLS+ S  PF++LD+ +LR+I +++  + AW+ A
Sbjct: 87  HVQATVVCAKSNGIQIRIRSGGHDYEGLSFTSSVPFVILDMHDLRSITIDVFRKQAWVDA 146

Query: 129 GATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDNIVDAQIVN 188
           GATMGELY  IA  S   AF GGVCPTLGAGGH+ GGGYGNL+RK+G+SVD++VDA+IV+
Sbjct: 147 GATMGELYTKIAAASKTLAFAGGVCPTLGAGGHISGGGYGNLIRKYGISVDHVVDARIVD 206

Query: 189 VQGHILNRQQMGDDLFWAIRGGGG-SFGVILSWKIKLVPVPSIVTVFNVDRTIEEGATNV 248
           V G+IL    +G DL WAIRGGGG SFGVILSWKI LV VP  VTVF V++T+E+G T+V
Sbjct: 207 VNGNILTGATLGRDLLWAIRGGGGASFGVILSWKINLVDVPKTVTVFKVNKTLEQGVTDV 266

[Close]

BLAST of MC01g_new0489.1 vs. TAIR 10
Match: AT1G26400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 294.3 bits (752), Expect = 9.3e-80
Identity = 141/244 (57.79%), Postives = 186/244 (76.23%), Query Frame = 0


Query: 7   DEQNFLQCLSSNTQSNFPISEAIFTP-NNSSFLPTLNFYIRNPRFQTPQTPKPLVIIAAK 66
           D   F  CL + T    PI++AIFT  N ++FL +   Y +N R+ +    K + I+AAK
Sbjct: 25  DSGIFTGCLRNRTSLENPITDAIFTSRNTTTFLSSYVSYTKNKRYSSLNYQKLVAIVAAK 84

Query: 67  HESHVQTTVICAKRVGLQIRIRSGGHDYEGLSYVSQQPFIVLDLFNLRAINVNISNQSAW 126
           H SHVQ TV+CAK  G+Q+RIRSGGHDYEGLSY S  PF++LD++NLR+I V++S++ AW
Sbjct: 85  HVSHVQATVVCAKANGIQLRIRSGGHDYEGLSYTSSVPFVILDMYNLRSITVDVSSKKAW 144

Query: 127 IQAGATMGELYYAIAKRSGVHAFPGGVCPTLGAGGHLLGGGYGNLMRKFGLSVDNIVDAQ 186
           +QAGAT+GELY  I + S   AFP GVCPT+G GGH+ GGG+GNLMRKFG++VD+++DAQ
Sbjct: 145 VQAGATLGELYTKINEASQTLAFPAGVCPTVGVGGHITGGGFGNLMRKFGITVDHVIDAQ 204

Query: 187 IVNVQGHILNRQQMGDDLFWAIRGGGG-SFGVILSWKIKLVPVPSIVTVFNVDRTIEEGA 246
           ++ V G +L+R  MG+DLFWAIRGGGG SFGVILSWKI LV VP I+TVF V +T+E+G 
Sbjct: 205 LIGVNGKLLDRATMGEDLFWAIRGGGGASFGVILSWKINLVEVPKILTVFKVSKTLEQGG 264

Query: 247 TNVV 249
           T+V+
Sbjct: 265 TDVL 268

The following BLAST results are available for this feature:

BLAST of MC01g_new0489.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FZC5	8.2e-81	60.58	Berberine bridge enzyme-like 4 OS=Arabidopsis thaliana OX=3702 GN=FOX2 PE=2 SV=1	[more]
Q9FZC7	2.4e-80	59.00	Berberine bridge enzyme-like 6 OS=Arabidopsis thaliana OX=3702 GN=FOX4 PE=2 SV=1	[more]
Q9FZC8	2.4e-80	58.61	Berberine bridge enzyme-like 7 OS=Arabidopsis thaliana OX=3702 GN=FOX5 PE=3 SV=1	[more]
Q9SVG7	4.5e-79	59.75	Berberine bridge enzyme-like 17 OS=Arabidopsis thaliana OX=3702 GN=At4g20800 PE=...	[more]
Q9FZC6	1.3e-78	57.79	Berberine bridge enzyme-like 5 OS=Arabidopsis thaliana OX=3702 GN=FOX3 PE=3 SV=1	[more]

BLAST of MC01g_new0489.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146700.1	6.44e-174	100.00	LOW QUALITY PROTEIN: berberine bridge enzyme-like 14 [Momordica charantia]	[more]
XP_022159193.1	4.39e-153	89.16	berberine bridge enzyme-like 14 [Momordica charantia]	[more]
XP_038883273.1	2.79e-139	83.47	berberine bridge enzyme-like 14 [Benincasa hispida]	[more]
XP_008441073.1	4.40e-135	81.40	PREDICTED: flavin-dependent oxidoreductase FOX2-like [Cucumis melo]	[more]
XP_008439524.1	4.54e-132	78.51	PREDICTED: flavin-dependent oxidoreductase FOX2-like [Cucumis melo]	[more]

BLAST of MC01g_new0489.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CY03	3.12e-174	100.00	LOW QUALITY PROTEIN: berberine bridge enzyme-like 14 OS=Momordica charantia OX=3...	[more]
A0A6J1DY47	2.13e-153	89.16	berberine bridge enzyme-like 14 OS=Momordica charantia OX=3673 GN=LOC111025613 P...	[more]
A0A1S3B2M2	2.13e-135	81.40	flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo OX=3656 GN=LOC10348529...	[more]
A0A1S3AYX7	2.20e-132	78.51	flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo OX=3656 GN=LOC10348429...	[more]
A0A5A7UD95	4.41e-132	78.51	Flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo var. makuwa OX=1194695...	[more]

BLAST of MC01g_new0489.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G26390.1	5.8e-82	60.58	FAD-binding Berberine family protein	[more]
AT1G26420.1	1.7e-81	58.61	FAD-binding Berberine family protein	[more]
AT1G26410.1	1.7e-81	59.00	FAD-binding Berberine family protein	[more]
AT4G20800.1	3.2e-80	59.75	FAD-binding Berberine family protein	[more]
AT1G26400.1	9.3e-80	57.79	FAD-binding Berberine family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006094	FAD linked oxidase, N-terminal	PFAM	PF01565	FAD_binding_4	coord: 58..194 e-value: 1.9E-24 score: 86.0
IPR016167	FAD-binding, type PCMH, subdomain 1	GENE3D	3.30.43.10		coord: 6..112 e-value: 1.3E-45 score: 155.6
IPR016169	FAD-binding, type PCMH, subdomain 2	GENE3D	3.30.465.10		coord: 116..229 e-value: 4.1E-63 score: 216.0
None	No IPR available	GENE3D	3.40.462.20		coord: 230..247 e-value: 4.1E-63 score: 216.0
None	No IPR available	PANTHER	PTHR32448	OS08G0158400 PROTEIN	coord: 6..248
None	No IPR available	PANTHER	PTHR32448:SF16	BERBERINE BRIDGE ENZYME-LIKE 17	coord: 6..248
IPR016166	FAD-binding domain, PCMH-type	PROSITE	PS51387	FAD_PCMH	coord: 54..227 score: 17.682079
IPR036318	FAD-binding, type PCMH-like superfamily	SUPERFAMILY	56176	FAD-binding/transporter-associated domain-like	coord: 9..228

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC01g_new0489	MC01g_new0489	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC01g_new0489.1-cds	MC01g_new0489.1-cds-MC01:20394348..20395094	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC01g_new0489.1	MC01g_new0489.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0008270	zinc ion binding