MC01g1456.1 (mRNA) Bitter gourd (Dali-11) v1

BLAST of MC01g1456.1 vs. ExPASy Swiss-Prot
Match: Q9FJ41 (GDSL esterase/lipase At5g45950 OS=Arabidopsis thaliana OX=3702 GN=At5g45950 PE=2 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 5.2e-108
Identity = 187/338 (55.33%), Postives = 248/338 (73.37%), Query Frame = 0

Query: 28  LMDVDVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTTMKSNFPPYGKDFFNARPTGR 87
           ++ +DV  LR+LA+K+NVTS+LVFGDSSVDPGNNN + T MK NFPPYG++F N +PTGR
Sbjct: 21  VLSLDVHLLRQLAAKHNVTSILVFGDSSVDPGNNNFIKTEMKGNFPPYGENFINHKPTGR 80

Query: 88  FCDGRLATDFIAEALGFGETVPAFLDTTLRPIHLLRGASFASASSGYDDLTANFSNVLSF 147
            CDG LA D+IAEA+G+   +PAFLD +L    L RGASFASA SGYDDLTAN SNV SF
Sbjct: 81  LCDGLLAPDYIAEAMGY-PPIPAFLDPSLTQADLTRGASFASAGSGYDDLTANISNVWSF 140

Query: 148 PKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVVISMGTNDFLENYFLEPLRPKQFSLDQY 207
             Q  YF+HYK+HL + VG  E+ K I NAI ++SMG+NDFL+NY ++  R KQF+++QY
Sbjct: 141 TTQANYFLHYKIHLTKLVGPLESAKMINNAIFLMSMGSNDFLQNYLVDFTRQKQFTVEQY 200

Query: 208 QNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGCMPLVRTITNQNTTCSKSFNQAAYAFNA 267
             FL   M  + +++HRLG +R+VVVGVPP+GCMPL++ +  Q  TC    NQ A++FNA
Sbjct: 201 IEFLSHRMLYDAKMLHRLGAKRLVVVGVPPMGCMPLIKYLRGQK-TCVDQLNQIAFSFNA 260

Query: 268 KMKLKLAAIKANLGMLTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEYGGTCKGS 327
           K+   L  +++ +G+ T +VDAY+ +Q+A+ NP  +G  E + GCCGTG  EYG TCK  
Sbjct: 261 KIIKNLELLQSKIGLKTIYVDAYSTIQEAIKNPRKFGFVEASLGCCGTGTYEYGETCKDM 320

Query: 328 STCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSVQRDIL 366
             C DP KYVFWDAVHP+++MY+II  +AI S+  + L
Sbjct: 321 QVCKDPTKYVFWDAVHPTQRMYQIIVKKAIASISEEFL 356

BLAST of MC01g1456.1 vs. ExPASy Swiss-Prot
Match: Q9FJ40 (GDSL esterase/lipase At5g45960 OS=Arabidopsis thaliana OX=3702 GN=At5g45960 PE=2 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 4.5e-75
Identity = 141/316 (44.62%), Postives = 200/316 (63.29%), Query Frame = 0

Query: 42  KYNVTSLLVFGDSSVDPGNNNVLSTTMKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEA 101
           K++V+++LVFGDS+VDPGNNN + T  K NFPPYG DF N  PTGRFC+GRL TDFIA  
Sbjct: 42  KHSVSAILVFGDSTVDPGNNNYIDTVFKCNFPPYGLDFRNKTPTGRFCNGRLVTDFIASY 101

Query: 102 LGFGETVPAFLDTTLRPIHLLRGASFASASSGYDDLTANFSNVLSFPKQLEYFMHYKLHL 161
           +G  E VP +LD  L    L+ G SFASA SGYD LT   +NV+  P QLEYF  YK  L
Sbjct: 102 IGVKENVPPYLDPNLGINELISGVSFASAGSGYDPLTPTITNVIDIPTQLEYFREYKRKL 161

Query: 162 GRQVGYEEAEKRIRNAIVVISMGTNDFLENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQL 221
             ++G +E EK I  A+  +S GTNDF+ NYF  P+R K F+++ YQ F++S++ + +Q 
Sbjct: 162 EGKMGKQEMEKHIEEAMFCVSAGTNDFVINYFTIPIRRKTFTIEAYQQFVISNLKQFIQG 221

Query: 222 MHRLGLRRVVVVGVPPLGCMPLVRTITN----QNTTCSKSFNQAAYAFNAKMKLKLAAIK 281
           + + G R++ V G+PP+GC+P+V T+ +     N  C   F+  A  +N  ++ +LA ++
Sbjct: 222 LWKEGARKITVAGLPPIGCLPIVITLFSGEALTNRRCIDRFSTVATNYNFLLQKQLALMQ 281

Query: 282 ---ANLGMLTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEYGGTCKGSS-TCSDP 341
              A+LG    ++D Y  V + + +P  +G  E   GCCG+G +E    C   S  C + 
Sbjct: 282 VGLAHLGSKIFYLDVYNPVYEVIRDPRKFGFEEVFSGCCGSGYLEASFLCNPKSYVCPNT 341

Query: 342 EKYVFWDAVHPSEKMY 350
             YVF+D++HPSEK Y
Sbjct: 342 SAYVFFDSIHPSEKTY 357

BLAST of MC01g1456.1 vs. ExPASy Swiss-Prot
Match: Q67ZI9 (GDSL esterase/lipase At2g42990 OS=Arabidopsis thaliana OX=3702 GN=At2g42990 PE=2 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.3e-71
Identity = 137/314 (43.63%), Postives = 201/314 (64.01%), Query Frame = 0

Query: 45  VTSLLVFGDSSVDPGNNNVLSTTMKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGF 104
           + +++VFGDSSVD GNNN +ST  ++NF PYG+DF   R TGRFC+GRL++DF +EA G 
Sbjct: 26  IPAIIVFGDSSVDSGNNNFISTMARANFEPYGRDFPGGRATGRFCNGRLSSDFTSEAYGL 85

Query: 105 GETVPAFLDTTLRPIHLLRGASFASASSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQ 164
             TVPA+LD +        G  FASA +GYD+ TA+   V+   K++EYF  Y+ +L   
Sbjct: 86  KPTVPAYLDPSYNISDFATGVCFASAGTGYDNSTADVLGVIPLWKEVEYFKEYQSNLSAY 145

Query: 165 VGYEEAEKRIRNAIVVISMGTNDFLENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHR 224
           +G+  A K IR ++ ++S+GTNDFLENY+  P R  QFS+ QYQ+FLV      ++ ++R
Sbjct: 146 LGHRRAAKIIRESLYIVSIGTNDFLENYYTLPDRRSQFSISQYQDFLVEIAEVFLKDIYR 205

Query: 225 LGLRRVVVVGVPPLGCMPLVRTITNQNT--TCSKSFNQAAYAFNAKMKLKLAAIKANL-G 284
           LG R++   G+ P+GC+PL R +TN +   +C++S+N  A  FN +++  +  +   L G
Sbjct: 206 LGARKMSFTGISPMGCLPLER-VTNLDDPFSCARSYNDLAVDFNGRLRRLVTKLNRELTG 265

Query: 285 MLTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEYGGTCKGSS--TCSDPEKYVFW 344
           +   F + Y I+ D V  P  YGL  ++  CCGTGL E G  C   +  TCSD  K+VFW
Sbjct: 266 IKIYFANPYDIMWDIVTKPNLYGLEISSSACCGTGLFEMGFLCGQDNPLTCSDANKFVFW 325

Query: 345 DAVHPSEKMYKIIA 354
           DA HP+E+  +I++
Sbjct: 326 DAFHPTERTNQIVS 338

BLAST of MC01g1456.1 vs. ExPASy Swiss-Prot
Match: Q9SJB4 (GDSL esterase/lipase At2g04570 OS=Arabidopsis thaliana OX=3702 GN=At2g04570 PE=2 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 3.4e-67
Identity = 131/320 (40.94%), Postives = 195/320 (60.94%), Query Frame = 0

Query: 45  VTSLLVFGDSSVDPGNNNVLSTTMKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGF 104
           + +++VFGDSSVD GNNN + T  +SNF PYG+DF   +PTGRFC+G++ATDF++EALG 
Sbjct: 26  IPAIIVFGDSSVDAGNNNYIPTVARSNFEPYGRDFVGGKPTGRFCNGKIATDFMSEALGL 85

Query: 105 GETVPAFLDTTLRPIHLLRGASFASASSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQ 164
              +PA+LD +        G +FASA++GYD+ T++  +VL   KQLEY+  Y+  L   
Sbjct: 86  KPIIPAYLDPSYNISDFATGVTFASAATGYDNATSDVLSVLPLWKQLEYYKEYQTKLKAY 145

Query: 165 VGYEEAEKRIRNAIVVISMGTNDFLENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHR 224
            G +   + I +++ +IS+GTNDFLENYF  P R  Q+S+  YQ+FL       V+ +H 
Sbjct: 146 QGKDRGTETIESSLYLISIGTNDFLENYFAFPGRSSQYSVSLYQDFLAGIAKEFVKKLHG 205

Query: 225 LGLRRVVVVGVPPLGCMPLVRTITNQNT--TCSKSFNQAAYAFNAKMKLKLAAIKANL-G 284
           LG R++ + G+PP+GCMPL R  TN  T   C   +N  A  FN+K+   +  +   L G
Sbjct: 206 LGARKISLGGLPPMGCMPLERA-TNIGTGGECVGRYNDIAVQFNSKLDKMVEKLSKELPG 265

Query: 285 MLTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEYGGTCKGSS--TCSDPEKYVFW 344
               F + Y      + NP+++G       CC TG+ E G  C+ ++  TC++ +KYVFW
Sbjct: 266 SNLVFSNPYEPFMRIIKNPSSFGFEVVGAACCATGMFEMGYGCQRNNPFTCTNADKYVFW 325

Query: 345 DAVHPSEKMYKIIAAEAIQS 360
           D+ HP++K   I+A   + S
Sbjct: 326 DSFHPTQKTNHIMANALMNS 344

BLAST of MC01g1456.1 vs. ExPASy Swiss-Prot
Match: Q9SIZ6 (GDSL esterase/lipase At2g40250 OS=Arabidopsis thaliana OX=3702 GN=At2g40250 PE=2 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 3.8e-66
Identity = 136/348 (39.08%), Postives = 207/348 (59.48%), Query Frame = 0

Query: 22  LSFCVPLMDVDVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTTMKSNFPPYGKDFFN 81
           ++F + ++ + +  L   +    +T+L  FGDS+VD GNNN + T  +SN PPYGK F +
Sbjct: 11  VTFLINILLLQLLNLTNASPSPPITALYAFGDSTVDSGNNNYIPTLFQSNHPPYGKSFPS 70

Query: 82  ARPTGRFCDGRLATDFIAEALGFGETVPAFLDTTLRPIHLLRGASFASASSGYDDLTANF 141
              TGRF DG+LATDFI  +LG   T+PA+L+ +++P+ LL G SFASA  G DD TA  
Sbjct: 71  KLSTGRFSDGKLATDFIVSSLGLKPTLPAYLNPSVKPVDLLTGVSFASAGGGLDDRTAKS 130

Query: 142 SNVLSFPKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVVISMGTNDFLENYFLEPLRPKQ 201
           S  ++  KQ  YF      +   VG  E  + I+NA+ VIS GTND + N + + +    
Sbjct: 131 SLTITMDKQWSYFEEALGKMKSLVGDSETNRVIKNAVFVISAGTNDMIFNVY-DHVLGSL 190

Query: 202 FSLDQYQNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGCMPLVRTITNQNT-------TC 261
            S+  YQ+ L++ +   VQ ++  G RR+ + G+PP+GC+P+  T+T+ NT        C
Sbjct: 191 ISVSDYQDSLLTKVEVFVQRLYEAGARRITIAGLPPIGCLPVQVTLTSINTPRIFHHRIC 250

Query: 262 SKSFNQAAYAFNAKM-KLKLAAIKANLGMLTSFVDAYAIVQDAVDNPTAYGLRETAKGCC 321
           ++  N  +  +N K+ KL     +   G    ++D Y+ + D + +P  YGL ET +GCC
Sbjct: 251 TEHQNDDSRVYNQKLQKLIFGLSQRFRGSKVLYLDIYSPLIDMIKHPRKYGLEETLRGCC 310

Query: 322 GTGLVEYGGTCKG-SSTCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSV 361
           GTGL+E G  C+  S TC D  KY+F+D+VHPS+  Y +IA+ A+Q +
Sbjct: 311 GTGLLEAGPLCQPLSRTCDDVSKYLFFDSVHPSQTAYSVIASFALQKL 357

BLAST of MC01g1456.1 vs. NCBI nr
Match: XP_022142093.1 (GDSL esterase/lipase At5g45950 [Momordica charantia])

HSP 1 Score: 728 bits (1878), Expect = 1.29e-264
Identity = 366/366 (100.00%), Postives = 366/366 (100.00%), Query Frame = 0

Query: 1   MMGQWGLRMRALLLAMGLTLRLSFCVPLMDVDVQQLRKLASKYNVTSLLVFGDSSVDPGN 60
           MMGQWGLRMRALLLAMGLTLRLSFCVPLMDVDVQQLRKLASKYNVTSLLVFGDSSVDPGN
Sbjct: 1   MMGQWGLRMRALLLAMGLTLRLSFCVPLMDVDVQQLRKLASKYNVTSLLVFGDSSVDPGN 60

Query: 61  NNVLSTTMKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDTTLRPIH 120
           NNVLSTTMKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDTTLRPIH
Sbjct: 61  NNVLSTTMKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDTTLRPIH 120

Query: 121 LLRGASFASASSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVV 180
           LLRGASFASASSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVV
Sbjct: 121 LLRGASFASASSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVV 180

Query: 181 ISMGTNDFLENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGC 240
           ISMGTNDFLENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGC
Sbjct: 181 ISMGTNDFLENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGC 240

Query: 241 MPLVRTITNQNTTCSKSFNQAAYAFNAKMKLKLAAIKANLGMLTSFVDAYAIVQDAVDNP 300
           MPLVRTITNQNTTCSKSFNQAAYAFNAKMKLKLAAIKANLGMLTSFVDAYAIVQDAVDNP
Sbjct: 241 MPLVRTITNQNTTCSKSFNQAAYAFNAKMKLKLAAIKANLGMLTSFVDAYAIVQDAVDNP 300

Query: 301 TAYGLRETAKGCCGTGLVEYGGTCKGSSTCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSV 360
           TAYGLRETAKGCCGTGLVEYGGTCKGSSTCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSV
Sbjct: 301 TAYGLRETAKGCCGTGLVEYGGTCKGSSTCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSV 360

Query: 361 QRDILS 366
           QRDILS
Sbjct: 361 QRDILS 366

BLAST of MC01g1456.1 vs. NCBI nr
Match: XP_038881090.1 (GDSL esterase/lipase At5g45950 [Benincasa hispida])

HSP 1 Score: 603 bits (1554), Expect = 3.77e-215
Identity = 302/356 (84.83%), Postives = 324/356 (91.01%), Query Frame = 0

Query: 22  LSFCVPLMD-----------VDVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTTMKS 81
           +SFC  LM+           V+V+QLRK+A KYNVTSLLVFGDSSVDPGNNN+LSTTMKS
Sbjct: 17  ISFCPSLMEGKMENDDFIGEVNVKQLRKMAWKYNVTSLLVFGDSSVDPGNNNLLSTTMKS 76

Query: 82  NFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDTTLRPIHLLRGASFASA 141
           NFPPYGKDFFNA PTGRFCDGRLATDFIAEALGFGETVPAFLD TL+PI LL G SFASA
Sbjct: 77  NFPPYGKDFFNALPTGRFCDGRLATDFIAEALGFGETVPAFLDRTLKPIDLLHGVSFASA 136

Query: 142 SSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVVISMGTNDFLE 201
           SSGYDDLTANFSNVLS PKQLEY MHYKLHL RQVG+E+AEK IRNAIVVISMGTNDFLE
Sbjct: 137 SSGYDDLTANFSNVLSLPKQLEYLMHYKLHLSRQVGHEKAEKIIRNAIVVISMGTNDFLE 196

Query: 202 NYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGCMPLVRTITNQ 261
           NYFLEPLRPKQFSLDQYQNFLV+SM  NVQ+MHRLG+RR+VVVGVPPLGCMP+VRTITNQ
Sbjct: 197 NYFLEPLRPKQFSLDQYQNFLVTSMYHNVQVMHRLGVRRLVVVGVPPLGCMPVVRTITNQ 256

Query: 262 NTTCSKSFNQAAYAFNAKMKLKLAAIKANLGMLTSFVDAYAIVQDAVDNPTAYGLRETAK 321
           NTTCSK FNQAAYAFNAKMKL LAAIKANLGMLTSFVDAYAI+Q AV NPTAYGL+ETAK
Sbjct: 257 NTTCSKPFNQAAYAFNAKMKLNLAAIKANLGMLTSFVDAYAILQAAVQNPTAYGLKETAK 316

Query: 322 GCCGTGLVEYGGTCKGSSTCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSVQRDILS 366
           GCCGTGLVEYG TCKGS+TC+DPE YVFWDAVHPS+KMYKI+AA+AI SVQ+DILS
Sbjct: 317 GCCGTGLVEYGETCKGSTTCNDPETYVFWDAVHPSQKMYKILAAQAILSVQQDILS 372

BLAST of MC01g1456.1 vs. NCBI nr
Match: XP_004134938.1 (GDSL esterase/lipase At5g45950 [Cucumis sativus] >KGN49277.1 hypothetical protein Csa_004133 [Cucumis sativus])

HSP 1 Score: 603 bits (1554), Expect = 3.91e-215
Identity = 305/357 (85.43%), Postives = 324/357 (90.76%), Query Frame = 0

Query: 22  LSFCVPLMD------------VDVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTTMK 81
           LSFC  LM+            V+V+QLRKLA KYNVTSLLVFGDSSVDPGNNN LSTTMK
Sbjct: 17  LSFCPSLMEGKKENSNDFIGEVNVKQLRKLAWKYNVTSLLVFGDSSVDPGNNNFLSTTMK 76

Query: 82  SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDTTLRPIHLLRGASFAS 141
           SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLD TL+PI LL G SFAS
Sbjct: 77  SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDRTLKPIELLHGVSFAS 136

Query: 142 ASSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVVISMGTNDFL 201
           ASSGYDDLTAN+SNVLS PKQLEY MHYKLHL RQVG E+AEK I+NAIVVISMGTNDFL
Sbjct: 137 ASSGYDDLTANYSNVLSLPKQLEYLMHYKLHLKRQVGGEKAEKIIKNAIVVISMGTNDFL 196

Query: 202 ENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGCMPLVRTITN 261
           ENYFLEPLRPKQFSLDQYQNFLVSSM RNVQ+MHRLG+RR+VVVGVPPLGCMP+VRTITN
Sbjct: 197 ENYFLEPLRPKQFSLDQYQNFLVSSMYRNVQVMHRLGVRRLVVVGVPPLGCMPVVRTITN 256

Query: 262 QNTTCSKSFNQAAYAFNAKMKLKLAAIKANLGMLTSFVDAYAIVQDAVDNPTAYGLRETA 321
           QNTTCS+ FNQAAYAFNAKMKLKLA IKA+LGMLTSFVDAYAIVQ AV NPTAYGLRETA
Sbjct: 257 QNTTCSEVFNQAAYAFNAKMKLKLAGIKASLGMLTSFVDAYAIVQAAVHNPTAYGLRETA 316

Query: 322 KGCCGTGLVEYGGTCKGSSTCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSVQRDILS 366
           KGCCGTGLVEYG TCKGS TCSDPE Y+FWDAVHPSEKMYKI+AA+AI+SVQ++ILS
Sbjct: 317 KGCCGTGLVEYGETCKGSPTCSDPENYLFWDAVHPSEKMYKILAAQAIRSVQQNILS 373

BLAST of MC01g1456.1 vs. NCBI nr
Match: KAA0052645.1 (GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK13181.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 602 bits (1551), Expect = 1.20e-214
Identity = 303/357 (84.87%), Postives = 321/357 (89.92%), Query Frame = 0

Query: 22  LSFCVPLM------------DVDVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTTMK 81
           L FC  LM            DV+V+QLRK+A KYNVTSLLVFGDSSVDPGNNN L TTMK
Sbjct: 18  LGFCPSLMEGKKENSNDFIGDVNVKQLRKMAWKYNVTSLLVFGDSSVDPGNNNFLPTTMK 77

Query: 82  SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDTTLRPIHLLRGASFAS 141
           SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLD TL+PI LL G SFAS
Sbjct: 78  SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDRTLKPIELLHGVSFAS 137

Query: 142 ASSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVVISMGTNDFL 201
           ASSGYDDLTAN+SNVLS PKQLEY MHYKLHL RQVG E+AEK IRNAIVVISMGTNDFL
Sbjct: 138 ASSGYDDLTANYSNVLSLPKQLEYLMHYKLHLKRQVGGEKAEKIIRNAIVVISMGTNDFL 197

Query: 202 ENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGCMPLVRTITN 261
           ENYFLEPLRPKQF+LDQYQNFLVSSM RNVQ+MHRLG+RR+VVVGVPPLGCMP+VRTITN
Sbjct: 198 ENYFLEPLRPKQFTLDQYQNFLVSSMYRNVQVMHRLGVRRLVVVGVPPLGCMPVVRTITN 257

Query: 262 QNTTCSKSFNQAAYAFNAKMKLKLAAIKANLGMLTSFVDAYAIVQDAVDNPTAYGLRETA 321
           QNTTCSK FNQAAYAFNAKMKLKLA IKA+LGMLTSFVDAYAIVQ AV NPTAYGLRETA
Sbjct: 258 QNTTCSKVFNQAAYAFNAKMKLKLAGIKASLGMLTSFVDAYAIVQAAVQNPTAYGLRETA 317

Query: 322 KGCCGTGLVEYGGTCKGSSTCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSVQRDILS 366
           +GCCGTGLVEYG TCKGS TC+DP+ YVFWDAVHPS+KMYKI+AA+AIQSVQ DILS
Sbjct: 318 RGCCGTGLVEYGETCKGSPTCTDPQNYVFWDAVHPSQKMYKILAAQAIQSVQHDILS 374

BLAST of MC01g1456.1 vs. NCBI nr
Match: XP_008439783.1 (PREDICTED: GDSL esterase/lipase At5g45950 [Cucumis melo])

HSP 1 Score: 602 bits (1551), Expect = 1.20e-214
Identity = 303/357 (84.87%), Postives = 321/357 (89.92%), Query Frame = 0

Query: 22  LSFCVPLM------------DVDVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTTMK 81
           L FC  LM            DV+V+QLRK+A KYNVTSLLVFGDSSVDPGNNN L TTMK
Sbjct: 18  LGFCPSLMEGKKENSNDFIGDVNVKQLRKMAWKYNVTSLLVFGDSSVDPGNNNFLPTTMK 77

Query: 82  SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDTTLRPIHLLRGASFAS 141
           SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLD TL+PI LL G SFAS
Sbjct: 78  SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDRTLKPIELLHGVSFAS 137

Query: 142 ASSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVVISMGTNDFL 201
           ASSGYDDLTAN+SNVLS PKQLEY MHYKLHL RQVG E+AEK IRNAIVVISMGTNDFL
Sbjct: 138 ASSGYDDLTANYSNVLSLPKQLEYLMHYKLHLKRQVGGEKAEKIIRNAIVVISMGTNDFL 197

Query: 202 ENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGCMPLVRTITN 261
           ENYFLEPLRPKQF+LDQYQNFLVSSM RNVQ+MHRLG+RR+VVVGVPPLGCMP+VRTITN
Sbjct: 198 ENYFLEPLRPKQFTLDQYQNFLVSSMYRNVQVMHRLGVRRLVVVGVPPLGCMPVVRTITN 257

Query: 262 QNTTCSKSFNQAAYAFNAKMKLKLAAIKANLGMLTSFVDAYAIVQDAVDNPTAYGLRETA 321
           QNTTCSK FNQAAYAFNAKMKLKLA IKA+LGMLTSFVDAYAIVQ AV NPTAYGLRETA
Sbjct: 258 QNTTCSKVFNQAAYAFNAKMKLKLAGIKASLGMLTSFVDAYAIVQAAVQNPTAYGLRETA 317

Query: 322 KGCCGTGLVEYGGTCKGSSTCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSVQRDILS 366
           +GCCGTGLVEYG TCKGS TC+DP+ YVFWDAVHPS+KMYKI+AA+AIQSVQ DILS
Sbjct: 318 RGCCGTGLVEYGETCKGSPTCTDPQNYVFWDAVHPSQKMYKILAAQAIQSVQHDILS 374

BLAST of MC01g1456.1 vs. ExPASy TrEMBL
Match: A0A6J1CMD4 (GDSL esterase/lipase At5g45950 OS=Momordica charantia OX=3673 GN=LOC111012307 PE=3 SV=1)

HSP 1 Score: 728 bits (1878), Expect = 6.25e-265
Identity = 366/366 (100.00%), Postives = 366/366 (100.00%), Query Frame = 0

Query: 1   MMGQWGLRMRALLLAMGLTLRLSFCVPLMDVDVQQLRKLASKYNVTSLLVFGDSSVDPGN 60
           MMGQWGLRMRALLLAMGLTLRLSFCVPLMDVDVQQLRKLASKYNVTSLLVFGDSSVDPGN
Sbjct: 1   MMGQWGLRMRALLLAMGLTLRLSFCVPLMDVDVQQLRKLASKYNVTSLLVFGDSSVDPGN 60

Query: 61  NNVLSTTMKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDTTLRPIH 120
           NNVLSTTMKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDTTLRPIH
Sbjct: 61  NNVLSTTMKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDTTLRPIH 120

Query: 121 LLRGASFASASSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVV 180
           LLRGASFASASSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVV
Sbjct: 121 LLRGASFASASSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVV 180

Query: 181 ISMGTNDFLENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGC 240
           ISMGTNDFLENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGC
Sbjct: 181 ISMGTNDFLENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGC 240

Query: 241 MPLVRTITNQNTTCSKSFNQAAYAFNAKMKLKLAAIKANLGMLTSFVDAYAIVQDAVDNP 300
           MPLVRTITNQNTTCSKSFNQAAYAFNAKMKLKLAAIKANLGMLTSFVDAYAIVQDAVDNP
Sbjct: 241 MPLVRTITNQNTTCSKSFNQAAYAFNAKMKLKLAAIKANLGMLTSFVDAYAIVQDAVDNP 300

Query: 301 TAYGLRETAKGCCGTGLVEYGGTCKGSSTCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSV 360
           TAYGLRETAKGCCGTGLVEYGGTCKGSSTCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSV
Sbjct: 301 TAYGLRETAKGCCGTGLVEYGGTCKGSSTCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSV 360

Query: 361 QRDILS 366
           QRDILS
Sbjct: 361 QRDILS 366

BLAST of MC01g1456.1 vs. ExPASy TrEMBL
Match: A0A0A0KLL4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G518320 PE=3 SV=1)

HSP 1 Score: 603 bits (1554), Expect = 1.89e-215
Identity = 305/357 (85.43%), Postives = 324/357 (90.76%), Query Frame = 0

Query: 22  LSFCVPLMD------------VDVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTTMK 81
           LSFC  LM+            V+V+QLRKLA KYNVTSLLVFGDSSVDPGNNN LSTTMK
Sbjct: 17  LSFCPSLMEGKKENSNDFIGEVNVKQLRKLAWKYNVTSLLVFGDSSVDPGNNNFLSTTMK 76

Query: 82  SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDTTLRPIHLLRGASFAS 141
           SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLD TL+PI LL G SFAS
Sbjct: 77  SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDRTLKPIELLHGVSFAS 136

Query: 142 ASSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVVISMGTNDFL 201
           ASSGYDDLTAN+SNVLS PKQLEY MHYKLHL RQVG E+AEK I+NAIVVISMGTNDFL
Sbjct: 137 ASSGYDDLTANYSNVLSLPKQLEYLMHYKLHLKRQVGGEKAEKIIKNAIVVISMGTNDFL 196

Query: 202 ENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGCMPLVRTITN 261
           ENYFLEPLRPKQFSLDQYQNFLVSSM RNVQ+MHRLG+RR+VVVGVPPLGCMP+VRTITN
Sbjct: 197 ENYFLEPLRPKQFSLDQYQNFLVSSMYRNVQVMHRLGVRRLVVVGVPPLGCMPVVRTITN 256

Query: 262 QNTTCSKSFNQAAYAFNAKMKLKLAAIKANLGMLTSFVDAYAIVQDAVDNPTAYGLRETA 321
           QNTTCS+ FNQAAYAFNAKMKLKLA IKA+LGMLTSFVDAYAIVQ AV NPTAYGLRETA
Sbjct: 257 QNTTCSEVFNQAAYAFNAKMKLKLAGIKASLGMLTSFVDAYAIVQAAVHNPTAYGLRETA 316

Query: 322 KGCCGTGLVEYGGTCKGSSTCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSVQRDILS 366
           KGCCGTGLVEYG TCKGS TCSDPE Y+FWDAVHPSEKMYKI+AA+AI+SVQ++ILS
Sbjct: 317 KGCCGTGLVEYGETCKGSPTCSDPENYLFWDAVHPSEKMYKILAAQAIRSVQQNILS 373

BLAST of MC01g1456.1 vs. ExPASy TrEMBL
Match: A0A5D3CMY5 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007930 PE=3 SV=1)

HSP 1 Score: 602 bits (1551), Expect = 5.83e-215
Identity = 303/357 (84.87%), Postives = 321/357 (89.92%), Query Frame = 0

Query: 22  LSFCVPLM------------DVDVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTTMK 81
           L FC  LM            DV+V+QLRK+A KYNVTSLLVFGDSSVDPGNNN L TTMK
Sbjct: 18  LGFCPSLMEGKKENSNDFIGDVNVKQLRKMAWKYNVTSLLVFGDSSVDPGNNNFLPTTMK 77

Query: 82  SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDTTLRPIHLLRGASFAS 141
           SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLD TL+PI LL G SFAS
Sbjct: 78  SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDRTLKPIELLHGVSFAS 137

Query: 142 ASSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVVISMGTNDFL 201
           ASSGYDDLTAN+SNVLS PKQLEY MHYKLHL RQVG E+AEK IRNAIVVISMGTNDFL
Sbjct: 138 ASSGYDDLTANYSNVLSLPKQLEYLMHYKLHLKRQVGGEKAEKIIRNAIVVISMGTNDFL 197

Query: 202 ENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGCMPLVRTITN 261
           ENYFLEPLRPKQF+LDQYQNFLVSSM RNVQ+MHRLG+RR+VVVGVPPLGCMP+VRTITN
Sbjct: 198 ENYFLEPLRPKQFTLDQYQNFLVSSMYRNVQVMHRLGVRRLVVVGVPPLGCMPVVRTITN 257

Query: 262 QNTTCSKSFNQAAYAFNAKMKLKLAAIKANLGMLTSFVDAYAIVQDAVDNPTAYGLRETA 321
           QNTTCSK FNQAAYAFNAKMKLKLA IKA+LGMLTSFVDAYAIVQ AV NPTAYGLRETA
Sbjct: 258 QNTTCSKVFNQAAYAFNAKMKLKLAGIKASLGMLTSFVDAYAIVQAAVQNPTAYGLRETA 317

Query: 322 KGCCGTGLVEYGGTCKGSSTCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSVQRDILS 366
           +GCCGTGLVEYG TCKGS TC+DP+ YVFWDAVHPS+KMYKI+AA+AIQSVQ DILS
Sbjct: 318 RGCCGTGLVEYGETCKGSPTCTDPQNYVFWDAVHPSQKMYKILAAQAIQSVQHDILS 374

BLAST of MC01g1456.1 vs. ExPASy TrEMBL
Match: A0A1S3AZJ4 (GDSL esterase/lipase At5g45950 OS=Cucumis melo OX=3656 GN=LOC103484475 PE=3 SV=1)

HSP 1 Score: 602 bits (1551), Expect = 5.83e-215
Identity = 303/357 (84.87%), Postives = 321/357 (89.92%), Query Frame = 0

Query: 22  LSFCVPLM------------DVDVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTTMK 81
           L FC  LM            DV+V+QLRK+A KYNVTSLLVFGDSSVDPGNNN L TTMK
Sbjct: 18  LGFCPSLMEGKKENSNDFIGDVNVKQLRKMAWKYNVTSLLVFGDSSVDPGNNNFLPTTMK 77

Query: 82  SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDTTLRPIHLLRGASFAS 141
           SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLD TL+PI LL G SFAS
Sbjct: 78  SNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDRTLKPIELLHGVSFAS 137

Query: 142 ASSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVVISMGTNDFL 201
           ASSGYDDLTAN+SNVLS PKQLEY MHYKLHL RQVG E+AEK IRNAIVVISMGTNDFL
Sbjct: 138 ASSGYDDLTANYSNVLSLPKQLEYLMHYKLHLKRQVGGEKAEKIIRNAIVVISMGTNDFL 197

Query: 202 ENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGCMPLVRTITN 261
           ENYFLEPLRPKQF+LDQYQNFLVSSM RNVQ+MHRLG+RR+VVVGVPPLGCMP+VRTITN
Sbjct: 198 ENYFLEPLRPKQFTLDQYQNFLVSSMYRNVQVMHRLGVRRLVVVGVPPLGCMPVVRTITN 257

Query: 262 QNTTCSKSFNQAAYAFNAKMKLKLAAIKANLGMLTSFVDAYAIVQDAVDNPTAYGLRETA 321
           QNTTCSK FNQAAYAFNAKMKLKLA IKA+LGMLTSFVDAYAIVQ AV NPTAYGLRETA
Sbjct: 258 QNTTCSKVFNQAAYAFNAKMKLKLAGIKASLGMLTSFVDAYAIVQAAVQNPTAYGLRETA 317

Query: 322 KGCCGTGLVEYGGTCKGSSTCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSVQRDILS 366
           +GCCGTGLVEYG TCKGS TC+DP+ YVFWDAVHPS+KMYKI+AA+AIQSVQ DILS
Sbjct: 318 RGCCGTGLVEYGETCKGSPTCTDPQNYVFWDAVHPSQKMYKILAAQAIQSVQHDILS 374

BLAST of MC01g1456.1 vs. ExPASy TrEMBL
Match: A0A6J1EEC9 (GDSL esterase/lipase At5g45950 OS=Cucurbita moschata OX=3662 GN=LOC111433547 PE=3 SV=1)

HSP 1 Score: 594 bits (1531), Expect = 5.39e-212
Identity = 298/361 (82.55%), Postives = 326/361 (90.30%), Query Frame = 0

Query: 12  LLLAMGLTLRLSFCVPLMD------VDVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLS 71
           LL  MG+     FC PLM+      V+VQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLS
Sbjct: 14  LLFIMGI----GFCPPLMEGKEIAEVNVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLS 73

Query: 72  TTMKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGFGETVPAFLDTTLRPIHLLRGA 131
           T +KSNFPPYGKDFFNARPTGRFCDGRLATDFIAEA+GFGETVPAFLD TL+PI LL G 
Sbjct: 74  TNVKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEAMGFGETVPAFLDRTLKPIDLLHGV 133

Query: 132 SFASASSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVVISMGT 191
           SFASASSGYDDLTAN S VLS PKQLEYFMHYK+HL R VGY++AE  IRNAIVV+SMGT
Sbjct: 134 SFASASSGYDDLTANLSKVLSLPKQLEYFMHYKIHLSRLVGYDKAENIIRNAIVVLSMGT 193

Query: 192 NDFLENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGCMPLVR 251
           NDFLENYFLEP R KQFSLDQYQNFLVSSMS NV++MHRLG+RR+VVVGVPPLGCMP+VR
Sbjct: 194 NDFLENYFLEPQRRKQFSLDQYQNFLVSSMSHNVEVMHRLGIRRLVVVGVPPLGCMPVVR 253

Query: 252 TITNQNTTCSKSFNQAAYAFNAKMKLKLAAIKANLGMLTSFVDAYAIVQDAVDNPTAYGL 311
           TITNQ TTCS SFN+AAYAFNAK+KLKLAA++A+LG+LTSFVDAYAIVQDAVDNPTAYGL
Sbjct: 254 TITNQITTCSTSFNRAAYAFNAKIKLKLAAMEAHLGLLTSFVDAYAIVQDAVDNPTAYGL 313

Query: 312 RETAKGCCGTGLVEYGGTCKGSSTCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSVQRDIL 366
           RETAKGCCGTGLVE+G TCKGS+TCSDP+ YVFWDAVHPS+ MYKI+AA+AIQSV +DIL
Sbjct: 314 RETAKGCCGTGLVEFGETCKGSTTCSDPQSYVFWDAVHPSQNMYKILAAKAIQSVAQDIL 370

BLAST of MC01g1456.1 vs. TAIR 10
Match: AT5G45950.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 392.5 bits (1007), Expect = 3.7e-109
Identity = 187/338 (55.33%), Postives = 248/338 (73.37%), Query Frame = 0

Query: 28  LMDVDVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTTMKSNFPPYGKDFFNARPTGR 87
           ++ +DV  LR+LA+K+NVTS+LVFGDSSVDPGNNN + T MK NFPPYG++F N +PTGR
Sbjct: 21  VLSLDVHLLRQLAAKHNVTSILVFGDSSVDPGNNNFIKTEMKGNFPPYGENFINHKPTGR 80

Query: 88  FCDGRLATDFIAEALGFGETVPAFLDTTLRPIHLLRGASFASASSGYDDLTANFSNVLSF 147
            CDG LA D+IAEA+G+   +PAFLD +L    L RGASFASA SGYDDLTAN SNV SF
Sbjct: 81  LCDGLLAPDYIAEAMGY-PPIPAFLDPSLTQADLTRGASFASAGSGYDDLTANISNVWSF 140

Query: 148 PKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVVISMGTNDFLENYFLEPLRPKQFSLDQY 207
             Q  YF+HYK+HL + VG  E+ K I NAI ++SMG+NDFL+NY ++  R KQF+++QY
Sbjct: 141 TTQANYFLHYKIHLTKLVGPLESAKMINNAIFLMSMGSNDFLQNYLVDFTRQKQFTVEQY 200

Query: 208 QNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGCMPLVRTITNQNTTCSKSFNQAAYAFNA 267
             FL   M  + +++HRLG +R+VVVGVPP+GCMPL++ +  Q  TC    NQ A++FNA
Sbjct: 201 IEFLSHRMLYDAKMLHRLGAKRLVVVGVPPMGCMPLIKYLRGQK-TCVDQLNQIAFSFNA 260

Query: 268 KMKLKLAAIKANLGMLTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEYGGTCKGS 327
           K+   L  +++ +G+ T +VDAY+ +Q+A+ NP  +G  E + GCCGTG  EYG TCK  
Sbjct: 261 KIIKNLELLQSKIGLKTIYVDAYSTIQEAIKNPRKFGFVEASLGCCGTGTYEYGETCKDM 320

Query: 328 STCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSVQRDIL 366
             C DP KYVFWDAVHP+++MY+II  +AI S+  + L
Sbjct: 321 QVCKDPTKYVFWDAVHPTQRMYQIIVKKAIASISEEFL 356

BLAST of MC01g1456.1 vs. TAIR 10
Match: AT5G45960.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 283.1 bits (723), Expect = 3.2e-76
Identity = 141/316 (44.62%), Postives = 200/316 (63.29%), Query Frame = 0

Query: 42  KYNVTSLLVFGDSSVDPGNNNVLSTTMKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEA 101
           K++V+++LVFGDS+VDPGNNN + T  K NFPPYG DF N  PTGRFC+GRL TDFIA  
Sbjct: 42  KHSVSAILVFGDSTVDPGNNNYIDTVFKCNFPPYGLDFRNKTPTGRFCNGRLVTDFIASY 101

Query: 102 LGFGETVPAFLDTTLRPIHLLRGASFASASSGYDDLTANFSNVLSFPKQLEYFMHYKLHL 161
           +G  E VP +LD  L    L+ G SFASA SGYD LT   +NV+  P QLEYF  YK  L
Sbjct: 102 IGVKENVPPYLDPNLGINELISGVSFASAGSGYDPLTPTITNVIDIPTQLEYFREYKRKL 161

Query: 162 GRQVGYEEAEKRIRNAIVVISMGTNDFLENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQL 221
             ++G +E EK I  A+  +S GTNDF+ NYF  P+R K F+++ YQ F++S++ + +Q 
Sbjct: 162 EGKMGKQEMEKHIEEAMFCVSAGTNDFVINYFTIPIRRKTFTIEAYQQFVISNLKQFIQG 221

Query: 222 MHRLGLRRVVVVGVPPLGCMPLVRTITN----QNTTCSKSFNQAAYAFNAKMKLKLAAIK 281
           + + G R++ V G+PP+GC+P+V T+ +     N  C   F+  A  +N  ++ +LA ++
Sbjct: 222 LWKEGARKITVAGLPPIGCLPIVITLFSGEALTNRRCIDRFSTVATNYNFLLQKQLALMQ 281

Query: 282 ---ANLGMLTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEYGGTCKGSS-TCSDP 341
              A+LG    ++D Y  V + + +P  +G  E   GCCG+G +E    C   S  C + 
Sbjct: 282 VGLAHLGSKIFYLDVYNPVYEVIRDPRKFGFEEVFSGCCGSGYLEASFLCNPKSYVCPNT 341

Query: 342 EKYVFWDAVHPSEKMY 350
             YVF+D++HPSEK Y
Sbjct: 342 SAYVFFDSIHPSEKTY 357

BLAST of MC01g1456.1 vs. TAIR 10
Match: AT2G42990.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 271.6 bits (693), Expect = 9.5e-73
Identity = 137/314 (43.63%), Postives = 201/314 (64.01%), Query Frame = 0

Query: 45  VTSLLVFGDSSVDPGNNNVLSTTMKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGF 104
           + +++VFGDSSVD GNNN +ST  ++NF PYG+DF   R TGRFC+GRL++DF +EA G 
Sbjct: 26  IPAIIVFGDSSVDSGNNNFISTMARANFEPYGRDFPGGRATGRFCNGRLSSDFTSEAYGL 85

Query: 105 GETVPAFLDTTLRPIHLLRGASFASASSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQ 164
             TVPA+LD +        G  FASA +GYD+ TA+   V+   K++EYF  Y+ +L   
Sbjct: 86  KPTVPAYLDPSYNISDFATGVCFASAGTGYDNSTADVLGVIPLWKEVEYFKEYQSNLSAY 145

Query: 165 VGYEEAEKRIRNAIVVISMGTNDFLENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHR 224
           +G+  A K IR ++ ++S+GTNDFLENY+  P R  QFS+ QYQ+FLV      ++ ++R
Sbjct: 146 LGHRRAAKIIRESLYIVSIGTNDFLENYYTLPDRRSQFSISQYQDFLVEIAEVFLKDIYR 205

Query: 225 LGLRRVVVVGVPPLGCMPLVRTITNQNT--TCSKSFNQAAYAFNAKMKLKLAAIKANL-G 284
           LG R++   G+ P+GC+PL R +TN +   +C++S+N  A  FN +++  +  +   L G
Sbjct: 206 LGARKMSFTGISPMGCLPLER-VTNLDDPFSCARSYNDLAVDFNGRLRRLVTKLNRELTG 265

Query: 285 MLTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEYGGTCKGSS--TCSDPEKYVFW 344
           +   F + Y I+ D V  P  YGL  ++  CCGTGL E G  C   +  TCSD  K+VFW
Sbjct: 266 IKIYFANPYDIMWDIVTKPNLYGLEISSSACCGTGLFEMGFLCGQDNPLTCSDANKFVFW 325

Query: 345 DAVHPSEKMYKIIA 354
           DA HP+E+  +I++
Sbjct: 326 DAFHPTERTNQIVS 338

BLAST of MC01g1456.1 vs. TAIR 10
Match: AT2G04570.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 256.9 bits (655), Expect = 2.4e-68
Identity = 131/320 (40.94%), Postives = 195/320 (60.94%), Query Frame = 0

Query: 45  VTSLLVFGDSSVDPGNNNVLSTTMKSNFPPYGKDFFNARPTGRFCDGRLATDFIAEALGF 104
           + +++VFGDSSVD GNNN + T  +SNF PYG+DF   +PTGRFC+G++ATDF++EALG 
Sbjct: 26  IPAIIVFGDSSVDAGNNNYIPTVARSNFEPYGRDFVGGKPTGRFCNGKIATDFMSEALGL 85

Query: 105 GETVPAFLDTTLRPIHLLRGASFASASSGYDDLTANFSNVLSFPKQLEYFMHYKLHLGRQ 164
              +PA+LD +        G +FASA++GYD+ T++  +VL   KQLEY+  Y+  L   
Sbjct: 86  KPIIPAYLDPSYNISDFATGVTFASAATGYDNATSDVLSVLPLWKQLEYYKEYQTKLKAY 145

Query: 165 VGYEEAEKRIRNAIVVISMGTNDFLENYFLEPLRPKQFSLDQYQNFLVSSMSRNVQLMHR 224
            G +   + I +++ +IS+GTNDFLENYF  P R  Q+S+  YQ+FL       V+ +H 
Sbjct: 146 QGKDRGTETIESSLYLISIGTNDFLENYFAFPGRSSQYSVSLYQDFLAGIAKEFVKKLHG 205

Query: 225 LGLRRVVVVGVPPLGCMPLVRTITNQNT--TCSKSFNQAAYAFNAKMKLKLAAIKANL-G 284
           LG R++ + G+PP+GCMPL R  TN  T   C   +N  A  FN+K+   +  +   L G
Sbjct: 206 LGARKISLGGLPPMGCMPLERA-TNIGTGGECVGRYNDIAVQFNSKLDKMVEKLSKELPG 265

Query: 285 MLTSFVDAYAIVQDAVDNPTAYGLRETAKGCCGTGLVEYGGTCKGSS--TCSDPEKYVFW 344
               F + Y      + NP+++G       CC TG+ E G  C+ ++  TC++ +KYVFW
Sbjct: 266 SNLVFSNPYEPFMRIIKNPSSFGFEVVGAACCATGMFEMGYGCQRNNPFTCTNADKYVFW 325

Query: 345 DAVHPSEKMYKIIAAEAIQS 360
           D+ HP++K   I+A   + S
Sbjct: 326 DSFHPTQKTNHIMANALMNS 344

BLAST of MC01g1456.1 vs. TAIR 10
Match: AT2G40250.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 253.4 bits (646), Expect = 2.7e-67
Identity = 136/348 (39.08%), Postives = 207/348 (59.48%), Query Frame = 0

Query: 22  LSFCVPLMDVDVQQLRKLASKYNVTSLLVFGDSSVDPGNNNVLSTTMKSNFPPYGKDFFN 81
           ++F + ++ + +  L   +    +T+L  FGDS+VD GNNN + T  +SN PPYGK F +
Sbjct: 11  VTFLINILLLQLLNLTNASPSPPITALYAFGDSTVDSGNNNYIPTLFQSNHPPYGKSFPS 70

Query: 82  ARPTGRFCDGRLATDFIAEALGFGETVPAFLDTTLRPIHLLRGASFASASSGYDDLTANF 141
              TGRF DG+LATDFI  +LG   T+PA+L+ +++P+ LL G SFASA  G DD TA  
Sbjct: 71  KLSTGRFSDGKLATDFIVSSLGLKPTLPAYLNPSVKPVDLLTGVSFASAGGGLDDRTAKS 130

Query: 142 SNVLSFPKQLEYFMHYKLHLGRQVGYEEAEKRIRNAIVVISMGTNDFLENYFLEPLRPKQ 201
           S  ++  KQ  YF      +   VG  E  + I+NA+ VIS GTND + N + + +    
Sbjct: 131 SLTITMDKQWSYFEEALGKMKSLVGDSETNRVIKNAVFVISAGTNDMIFNVY-DHVLGSL 190

Query: 202 FSLDQYQNFLVSSMSRNVQLMHRLGLRRVVVVGVPPLGCMPLVRTITNQNT-------TC 261
            S+  YQ+ L++ +   VQ ++  G RR+ + G+PP+GC+P+  T+T+ NT        C
Sbjct: 191 ISVSDYQDSLLTKVEVFVQRLYEAGARRITIAGLPPIGCLPVQVTLTSINTPRIFHHRIC 250

Query: 262 SKSFNQAAYAFNAKM-KLKLAAIKANLGMLTSFVDAYAIVQDAVDNPTAYGLRETAKGCC 321
           ++  N  +  +N K+ KL     +   G    ++D Y+ + D + +P  YGL ET +GCC
Sbjct: 251 TEHQNDDSRVYNQKLQKLIFGLSQRFRGSKVLYLDIYSPLIDMIKHPRKYGLEETLRGCC 310

Query: 322 GTGLVEYGGTCKG-SSTCSDPEKYVFWDAVHPSEKMYKIIAAEAIQSV 361
           GTGL+E G  C+  S TC D  KY+F+D+VHPS+  Y +IA+ A+Q +
Sbjct: 311 GTGLLEAGPLCQPLSRTCDDVSKYLFFDSVHPSQTAYSVIASFALQKL 357