MC01g1068.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC01g1068.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	protein TAPETUM DETERMINANT 1-like
Location	MC01: 16104559 .. 16105033 (+)
Sequence length	366
RNA-Seq Expression	MC01g1068.1
Synteny	MC01g1068.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTACCTTCGTCAAGCTTTTCACTGCACTTCTCTTCCTCTCTCTCCTTTCCAAAGGTATACAATATATTATTAACCAATATAATGTATTTCATTGAAATTTGTTCTTCTTGTTTATTACATTTCTAAGACTTCAATTATTATTATCATTTTTATTTTCCATCAGGGAATTGCCAGTGTTATTTGAACAACCTAACAATCAGCCAAAAGGAAACTGGATTTCAGATCCATGGAATGACAGAATGGAGAGTCACAATAAGCAACAATTGCCATTGTTCTCAATCGGACGTGAAATTGGATTGCACTGGATTTCAAACTACATTAAGCATTGATCCCACCATCCTAGCCATTTCTGACTCTGAGTGTTTGGTCAACAATGGGCAGCCAATTTTCAGTTCCAAACCCATCACTTTCACTTATGCATCGAACCCATCATTCCCTTTCAAGCCCATCTCTTCGCAAATTGCTTGTTCT

mRNA sequence

ATGGCTACCTTCGTCAAGCTTTTCACTGCACTTCTCTTCCTCTCTCTCCTTTCCAAAGGGAATTGCCAGTGTTATTTGAACAACCTAACAATCAGCCAAAAGGAAACTGGATTTCAGATCCATGGAATGACAGAATGGAGAGTCACAATAAGCAACAATTGCCATTGTTCTCAATCGGACGTGAAATTGGATTGCACTGGATTTCAAACTACATTAAGCATTGATCCCACCATCCTAGCCATTTCTGACTCTGAGTGTTTGGTCAACAATGGGCAGCCAATTTTCAGTTCCAAACCCATCACTTTCACTTATGCATCGAACCCATCATTCCCTTTCAAGCCCATCTCTTCGCAAATTGCTTGTTCT

Coding sequence (CDS)

ATGGCTACCTTCGTCAAGCTTTTCACTGCACTTCTCTTCCTCTCTCTCCTTTCCAAAGGGAATTGCCAGTGTTATTTGAACAACCTAACAATCAGCCAAAAGGAAACTGGATTTCAGATCCATGGAATGACAGAATGGAGAGTCACAATAAGCAACAATTGCCATTGTTCTCAATCGGACGTGAAATTGGATTGCACTGGATTTCAAACTACATTAAGCATTGATCCCACCATCCTAGCCATTTCTGACTCTGAGTGTTTGGTCAACAATGGGCAGCCAATTTTCAGTTCCAAACCCATCACTTTCACTTATGCATCGAACCCATCATTCCCTTTCAAGCCCATCTCTTCGCAAATTGCTTGTTCT

Protein sequence

MATFVKLFTALLFLSLLSKGNCQCYLNNLTISQKETGFQIHGMTEWRVTISNNCHCSQSDVKLDCTGFQTTLSIDPTILAISDSECLVNNGQPIFSSKPITFTYASNPSFPFKPISSQIACS

Homology

[Close]

BLAST of MC01g1068.1 vs. ExPASy Swiss-Prot
Match: Q1G3T1 (TPD1 protein homolog 1 OS=Arabidopsis thaliana OX=3702 GN=TDL1 PE=2 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 3.6e-05
Identity = 26/96 (27.08%), Postives = 51/96 (53.12%), Query Frame = 0


Query: 24  CYLNNLTISQKETGFQIHGMTEWRVTISNNC--HCSQSDVKLDCTGFQTTLSIDPTILAI 83
           C  +++ + Q  T     G+  + V I N+C   C+ +++ + C  F +   ++P +   
Sbjct: 80  CSKDDIVLFQGSTNPLPSGVPSYTVEIFNSCVSDCNIAEIHVSCGWFSSVRLVNPRVFRR 139

Query: 84  SD-SECLVNNGQPIFSSKPITFTYASNPSFPFKPIS 117
            D  +CLVN+GQP+   + ++F YA++ S+P    S
Sbjct: 140 LDYDDCLVNDGQPLGPGQSLSFQYANSFSYPLSVAS 175

[Close]

BLAST of MC01g1068.1 vs. NCBI nr
Match: XP_038881121.1 (uncharacterized protein At1g05835-like [Benincasa hispida])

HSP 1 Score: 196 bits (499), Expect = 1.69e-62
Identity = 94/122 (77.05%), Postives = 107/122 (87.70%), Query Frame = 0


Query: 1   MATFVKLFTALLFLSLLSKGNCQCYLNNLTISQKETGFQIHGMTEWRVTISNNCHCSQSD 60
           MA FVKL TALL LSL+S GNCQC LNN+TISQ+ TGFQIHGMTEW+VTISNNC CSQS+
Sbjct: 1   MAIFVKLVTALLVLSLVSTGNCQCILNNITISQRMTGFQIHGMTEWKVTISNNCPCSQSE 60

Query: 61  VKLDCTGFQTTLSIDPTILAISDSECLVNNGQPIFSSKPITFTYASNPSFPFKPISSQIA 120
           VK+DC GFQTT SIDP+ILA+ D+ECLVNNG PIF+S PITF+YA +P F FKPISSQI+
Sbjct: 61  VKIDCKGFQTTESIDPSILAVFDTECLVNNGLPIFNSNPITFSYAQDPQFNFKPISSQIS 120

Query: 121 CS 122
           CS
Sbjct: 121 CS 122

[Close]

BLAST of MC01g1068.1 vs. NCBI nr
Match: XP_031742370.1 (uncharacterized protein At1g05835-like [Cucumis sativus] >KGN48771.1 hypothetical protein Csa_003478 [Cucumis sativus])

HSP 1 Score: 195 bits (496), Expect = 4.85e-62
Identity = 93/122 (76.23%), Postives = 105/122 (86.07%), Query Frame = 0


Query: 1   MATFVKLFTALLFLSLLSKGNCQCYLNNLTISQKETGFQIHGMTEWRVTISNNCHCSQSD 60
           M  F KL  ALL LSL+S GNCQC+LNN+ ISQ  TGFQIHGMTEW+VTI+NNC CSQSD
Sbjct: 1   MPIFPKLLAALLVLSLISTGNCQCFLNNIKISQSMTGFQIHGMTEWKVTITNNCICSQSD 60

Query: 61  VKLDCTGFQTTLSIDPTILAISDSECLVNNGQPIFSSKPITFTYASNPSFPFKPISSQIA 120
           +KLDC GF TTLSIDP+ILAISDSECLVNNGQPIF+S+PITF YA +P F FKP+SSQI+
Sbjct: 61  LKLDCKGFATTLSIDPSILAISDSECLVNNGQPIFNSRPITFKYAQSPKFNFKPLSSQIS 120

Query: 121 CS 122
           CS
Sbjct: 121 CS 122

[Close]

BLAST of MC01g1068.1 vs. NCBI nr
Match: XP_008440637.1 (PREDICTED: protein TAPETUM DETERMINANT 1-like [Cucumis melo] >KAA0036281.1 protein TAPETUM DETERMINANT 1-like [Cucumis melo var. makuwa] >TYK12675.1 protein TAPETUM DETERMINANT 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 194 bits (492), Expect = 1.97e-61
Identity = 94/122 (77.05%), Postives = 104/122 (85.25%), Query Frame = 0


Query: 1   MATFVKLFTALLFLSLLSKGNCQCYLNNLTISQKETGFQIHGMTEWRVTISNNCHCSQSD 60
           MA  VKL  ALL LS +S GNCQC LNN+TISQK TGFQIHGMTEW VTI+NNC CSQSD
Sbjct: 1   MAISVKLLAALLVLSFISTGNCQCVLNNITISQKMTGFQIHGMTEWDVTITNNCICSQSD 60

Query: 61  VKLDCTGFQTTLSIDPTILAISDSECLVNNGQPIFSSKPITFTYASNPSFPFKPISSQIA 120
           +KLDC GF TTLSIDP+IL+ISD+ECLVNNG+PIF+SKPITF YA  P F FKPISSQI+
Sbjct: 61  LKLDCKGFATTLSIDPSILSISDNECLVNNGKPIFNSKPITFKYAQTPKFNFKPISSQIS 120

Query: 121 CS 122
           CS
Sbjct: 121 CS 122

[Close]

BLAST of MC01g1068.1 vs. NCBI nr
Match: KAG7026828.1 (Protein TAPETUM DETERMINANT 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 191 bits (485), Expect = 2.30e-60
Identity = 92/122 (75.41%), Postives = 103/122 (84.43%), Query Frame = 0


Query: 1   MATFVKLFTALLFLSLLSKGNCQCYLNNLTISQKETGFQIHGMTEWRVTISNNCHCSQSD 60
           MA F+KLF ALLF+SLL KGNCQC  N++ ISQK TGFQIHGMTEW VTISNNC CSQS+
Sbjct: 1   MAPFLKLFAALLFVSLLYKGNCQCVSNDIAISQKMTGFQIHGMTEWNVTISNNCLCSQSE 60

Query: 61  VKLDCTGFQTTLSIDPTILAISDSECLVNNGQPIFSSKPITFTYASNPSFPFKPISSQIA 120
           VKLDC GFQTT SIDP+ILA++D+ECLVNNG PIF S P+ FTYA +P F FK ISSQIA
Sbjct: 61  VKLDCKGFQTTKSIDPSILAVTDTECLVNNGLPIFKSNPVIFTYAQDPKFDFKLISSQIA 120

Query: 121 CS 122
           CS
Sbjct: 121 CS 122

[Close]

BLAST of MC01g1068.1 vs. NCBI nr
Match: XP_022962948.1 (uncharacterized protein At1g05835-like [Cucurbita moschata])

HSP 1 Score: 189 bits (480), Expect = 1.33e-59
Identity = 92/122 (75.41%), Postives = 101/122 (82.79%), Query Frame = 0


Query: 1   MATFVKLFTALLFLSLLSKGNCQCYLNNLTISQKETGFQIHGMTEWRVTISNNCHCSQSD 60
           MA   KLF ALLF+S LSK N QC+L+N+TI Q  TGFQIHGMTEW  T+SNNC CSQS+
Sbjct: 1   MAPSAKLFAALLFVSFLSKENSQCFLSNITIRQSMTGFQIHGMTEWNTTVSNNCLCSQSE 60

Query: 61  VKLDCTGFQTTLSIDPTILAISDSECLVNNGQPIFSSKPITFTYASNPSFPFKPISSQIA 120
           VKLDC GFQTT SIDP ILAI+D+ECLVNNG PIF+S PITFTYA NP F FKPISSQIA
Sbjct: 61  VKLDCRGFQTTKSIDPLILAITDTECLVNNGLPIFNSTPITFTYAQNPQFNFKPISSQIA 120

Query: 121 CS 122
           CS
Sbjct: 121 CS 122

[Close]

BLAST of MC01g1068.1 vs. ExPASy TrEMBL
Match: A0A0A0KLZ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G500630 PE=4 SV=1)

HSP 1 Score: 195 bits (496), Expect = 2.35e-62
Identity = 93/122 (76.23%), Postives = 105/122 (86.07%), Query Frame = 0


Query: 1   MATFVKLFTALLFLSLLSKGNCQCYLNNLTISQKETGFQIHGMTEWRVTISNNCHCSQSD 60
           M  F KL  ALL LSL+S GNCQC+LNN+ ISQ  TGFQIHGMTEW+VTI+NNC CSQSD
Sbjct: 1   MPIFPKLLAALLVLSLISTGNCQCFLNNIKISQSMTGFQIHGMTEWKVTITNNCICSQSD 60

Query: 61  VKLDCTGFQTTLSIDPTILAISDSECLVNNGQPIFSSKPITFTYASNPSFPFKPISSQIA 120
           +KLDC GF TTLSIDP+ILAISDSECLVNNGQPIF+S+PITF YA +P F FKP+SSQI+
Sbjct: 61  LKLDCKGFATTLSIDPSILAISDSECLVNNGQPIFNSRPITFKYAQSPKFNFKPLSSQIS 120

Query: 121 CS 122
           CS
Sbjct: 121 CS 122

[Close]

BLAST of MC01g1068.1 vs. ExPASy TrEMBL
Match: A0A5D3CMA8 (Protein TAPETUM DETERMINANT 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002630 PE=4 SV=1)

HSP 1 Score: 194 bits (492), Expect = 9.55e-62
Identity = 94/122 (77.05%), Postives = 104/122 (85.25%), Query Frame = 0


Query: 1   MATFVKLFTALLFLSLLSKGNCQCYLNNLTISQKETGFQIHGMTEWRVTISNNCHCSQSD 60
           MA  VKL  ALL LS +S GNCQC LNN+TISQK TGFQIHGMTEW VTI+NNC CSQSD
Sbjct: 1   MAISVKLLAALLVLSFISTGNCQCVLNNITISQKMTGFQIHGMTEWDVTITNNCICSQSD 60

Query: 61  VKLDCTGFQTTLSIDPTILAISDSECLVNNGQPIFSSKPITFTYASNPSFPFKPISSQIA 120
           +KLDC GF TTLSIDP+IL+ISD+ECLVNNG+PIF+SKPITF YA  P F FKPISSQI+
Sbjct: 61  LKLDCKGFATTLSIDPSILSISDNECLVNNGKPIFNSKPITFKYAQTPKFNFKPISSQIS 120

Query: 121 CS 122
           CS
Sbjct: 121 CS 122

[Close]

BLAST of MC01g1068.1 vs. ExPASy TrEMBL
Match: A0A1S3B1L8 (protein TAPETUM DETERMINANT 1-like OS=Cucumis melo OX=3656 GN=LOC103484995 PE=4 SV=1)

HSP 1 Score: 194 bits (492), Expect = 9.55e-62
Identity = 94/122 (77.05%), Postives = 104/122 (85.25%), Query Frame = 0


Query: 1   MATFVKLFTALLFLSLLSKGNCQCYLNNLTISQKETGFQIHGMTEWRVTISNNCHCSQSD 60
           MA  VKL  ALL LS +S GNCQC LNN+TISQK TGFQIHGMTEW VTI+NNC CSQSD
Sbjct: 1   MAISVKLLAALLVLSFISTGNCQCVLNNITISQKMTGFQIHGMTEWDVTITNNCICSQSD 60

Query: 61  VKLDCTGFQTTLSIDPTILAISDSECLVNNGQPIFSSKPITFTYASNPSFPFKPISSQIA 120
           +KLDC GF TTLSIDP+IL+ISD+ECLVNNG+PIF+SKPITF YA  P F FKPISSQI+
Sbjct: 61  LKLDCKGFATTLSIDPSILSISDNECLVNNGKPIFNSKPITFKYAQTPKFNFKPISSQIS 120

Query: 121 CS 122
           CS
Sbjct: 121 CS 122

[Close]

BLAST of MC01g1068.1 vs. ExPASy TrEMBL
Match: A0A6J1HGA0 (uncharacterized protein At1g05835-like OS=Cucurbita moschata OX=3662 GN=LOC111463301 PE=4 SV=1)

HSP 1 Score: 189 bits (480), Expect = 6.45e-60
Identity = 92/122 (75.41%), Postives = 101/122 (82.79%), Query Frame = 0


Query: 1   MATFVKLFTALLFLSLLSKGNCQCYLNNLTISQKETGFQIHGMTEWRVTISNNCHCSQSD 60
           MA   KLF ALLF+S LSK N QC+L+N+TI Q  TGFQIHGMTEW  T+SNNC CSQS+
Sbjct: 1   MAPSAKLFAALLFVSFLSKENSQCFLSNITIRQSMTGFQIHGMTEWNTTVSNNCLCSQSE 60

Query: 61  VKLDCTGFQTTLSIDPTILAISDSECLVNNGQPIFSSKPITFTYASNPSFPFKPISSQIA 120
           VKLDC GFQTT SIDP ILAI+D+ECLVNNG PIF+S PITFTYA NP F FKPISSQIA
Sbjct: 61  VKLDCRGFQTTKSIDPLILAITDTECLVNNGLPIFNSTPITFTYAQNPQFNFKPISSQIA 120

Query: 121 CS 122
           CS
Sbjct: 121 CS 122

[Close]

BLAST of MC01g1068.1 vs. ExPASy TrEMBL
Match: A0A6J1KP98 (protein TAPETUM DETERMINANT 1-like OS=Cucurbita maxima OX=3661 GN=LOC111497464 PE=4 SV=1)

HSP 1 Score: 179 bits (455), Expect = 4.16e-56
Identity = 88/122 (72.13%), Postives = 98/122 (80.33%), Query Frame = 0


Query: 1   MATFVKLFTALLFLSLLSKGNCQCYLNNLTISQKETGFQIHGMTEWRVTISNNCHCSQSD 60
           MA F KLF ALLF+SLL KGNCQC  N++ I Q  +GFQIHGMTEW VTISN C CSQS+
Sbjct: 1   MAPFAKLFAALLFVSLLHKGNCQCVSNDIAIRQTMSGFQIHGMTEWNVTISNICLCSQSE 60

Query: 61  VKLDCTGFQTTLSIDPTILAISDSECLVNNGQPIFSSKPITFTYASNPSFPFKPISSQIA 120
           VKLDC GFQTT SIDP++LA++DSECLVNNG PIF S  ITFTYA +P F FK ISSQI 
Sbjct: 61  VKLDCRGFQTTKSIDPSVLAVTDSECLVNNGLPIFKSNSITFTYAHDPKFDFKLISSQIN 120

Query: 121 CS 122
           CS
Sbjct: 121 CS 122

[Close]

BLAST of MC01g1068.1 vs. TAIR 10
Match: AT4G32090.1 (Beta-1,3-N-Acetylglucosaminyltransferase family protein )

HSP 1 Score: 82.4 bits (202), Expect = 2.7e-16
Identity = 42/119 (35.29%), Postives = 62/119 (52.10%), Query Frame = 0


Query: 1   MATFVKLFTALLFLSLLSKGNCQCYLNNLTISQKETGFQIHGMTEWRVTISNNCHCSQSD 60
           MA  +K F  +L LS++S G C C    + I    TG +I G  EW+VT+ N C+C Q  
Sbjct: 1   MAFALKCFVVVLLLSMVSHGLCLCTFGKIQIGAVRTGREIGGQPEWKVTVINTCNCFQKH 60

Query: 61  VKLDCTGFQTTLSIDPTILAISDSECLVNNGQPIFSSKPITFTYASNPSFPFKPISSQI 120
           V L C GF     + P +L    + CL+  G  + +     FTYA  P + F+P+ S++
Sbjct: 61  VTLSCGGFAPAKPVKPLLLQPQGNTCLMIKGAALPAGATAQFTYAGQP-YIFRPVGSKV 118

[Close]

BLAST of MC01g1068.1 vs. TAIR 10
Match: AT1G32583.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: tapetum determinant 1 (TAIR:AT4G24972.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 49.3 bits (116), Expect = 2.6e-06
Identity = 26/96 (27.08%), Postives = 51/96 (53.12%), Query Frame = 0


Query: 24  CYLNNLTISQKETGFQIHGMTEWRVTISNNC--HCSQSDVKLDCTGFQTTLSIDPTILAI 83
           C  +++ + Q  T     G+  + V I N+C   C+ +++ + C  F +   ++P +   
Sbjct: 80  CSKDDIVLFQGSTNPLPSGVPSYTVEIFNSCVSDCNIAEIHVSCGWFSSVRLVNPRVFRR 139

Query: 84  SD-SECLVNNGQPIFSSKPITFTYASNPSFPFKPIS 117
            D  +CLVN+GQP+   + ++F YA++ S+P    S
Sbjct: 140 LDYDDCLVNDGQPLGPGQSLSFQYANSFSYPLSVAS 175

The following BLAST results are available for this feature:

BLAST of MC01g1068.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q1G3T1	3.6e-05	27.08	TPD1 protein homolog 1 OS=Arabidopsis thaliana OX=3702 GN=TDL1 PE=2 SV=1	[more]

BLAST of MC01g1068.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881121.1	1.69e-62	77.05	uncharacterized protein At1g05835-like [Benincasa hispida]	[more]
XP_031742370.1	4.85e-62	76.23	uncharacterized protein At1g05835-like [Cucumis sativus] >KGN48771.1 hypothetica...	[more]
XP_008440637.1	1.97e-61	77.05	PREDICTED: protein TAPETUM DETERMINANT 1-like [Cucumis melo] >KAA0036281.1 prote...	[more]
KAG7026828.1	2.30e-60	75.41	Protein TAPETUM DETERMINANT 1, partial [Cucurbita argyrosperma subsp. argyrosper...	[more]
XP_022962948.1	1.33e-59	75.41	uncharacterized protein At1g05835-like [Cucurbita moschata]	[more]

BLAST of MC01g1068.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KLZ6	2.35e-62	76.23	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G500630 PE=4 SV=1	[more]
A0A5D3CMA8	9.55e-62	77.05	Protein TAPETUM DETERMINANT 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B1L8	9.55e-62	77.05	protein TAPETUM DETERMINANT 1-like OS=Cucumis melo OX=3656 GN=LOC103484995 PE=4 ...	[more]
A0A6J1HGA0	6.45e-60	75.41	uncharacterized protein At1g05835-like OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1KP98	4.16e-56	72.13	protein TAPETUM DETERMINANT 1-like OS=Cucurbita maxima OX=3661 GN=LOC111497464 P...	[more]

BLAST of MC01g1068.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G32090.1	2.7e-16	35.29	Beta-1,3-N-Acetylglucosaminyltransferase family protein	[more]
AT1G32583.1	2.6e-06	27.08	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR040361	Tapetum determinant 1	PANTHER	PTHR33184	PROTEIN TAPETUM DETERMINANT 1-LIKE-RELATED	coord: 1..122
None	No IPR available	PANTHER	PTHR33184:SF44	BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE FAMILY PROTEIN	coord: 1..122

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC01g1068	MC01g1068	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC01g1068.1-cds	MC01g1068.1-cds-MC01:16104559..16104616	CDS
MC01g1068.1-cds	MC01g1068.1-cds-MC01:16104726..16105033	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC01g1068.1	MC01g1068.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001709	cell fate determination