Lsi11G007800.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi11G007800.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	mediator of RNA polymerase II transcription subunit 22b-like
Location	chr11: 8972906 .. 8977362 (+)
Sequence length	1088
RNA-Seq Expression	Lsi11G007800.1
Synteny	Lsi11G007800.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGGATTTGTATTTTTGGTGGGCTAGTTTGTTGTAAGAGCCCATTAAGCAGCCATATCCAAGCCCATCTCTATTATTGAATTCCAAAGATTGTCTAGGGTTGCATCTACCTCTGTGGTGCTTCTTTGTAACTCCGGCAACGCCCATAAGCTCCGACCATCATCAACGACACGACCGCAGCTTCGACGAACTATCAGACACTTTCTAGCGAGGATCCAAGTCGATATTGGTGAGTTTTTGGATTTAGATCGTTTTGATTTAAGTTAAGATGCTGTAAAATAGTTTATAATCCTTTGAAACTCTTTGATTGTAGTTTGGACTTGATTCGAAGTTTTCGGATTGAGGTTTTAAGGTTGCTTTGGTTAAGATTACTCATTCTTTTAAGACTTAAGGTTTCTAACCAAGTTCTAACTTTGGAAGCTTGATTTTACTATTTTAGTAGTTTTTCATATAATTTTGAAAAAGATTGACTATTAGGATGTCTTATAAATTTTTGTTTGAAATCCTATCTTCGTTTTTAATCTTGTTGTGGTTTTCCTTGGGTTGCTTGGGTGGTTGTAATAGACTTGGTTTATTTATTTTGTTTTGAGGATTGGTTTGAGTTTGAATTGGTTAGTTATGTTACATGTTAGAATTTGGTAAAGTCTTGCTGAAGCTGGTTAGAAAGTGTTTTGAAGATTAGTTTTCTTGATTGATTTTGAAGTTAAAAACTTAAAATTGTTTATTAGGTGATTTGTAAGCGTGTAGTGTTTATGGTTACGTTGTCTTGCTGCCGAAATTCCTATTCACAGATAGTAGAGTTAGTCAAAGAGTAAATTTTGTTGATGTTCCCTAGTATAGTTTTAAATTTGGGGGTGTTACATTCTTCTTCCCCAATGTTTCTAATTCTCTTTCATTTCAATCACCTTGGCGCCATCGCAGAGCTGATTCAATTCCCATTCTATTATTATGAACAAAGGCATTGGTGGTGGAACTGGCCCCACTGCAGCGGCTGCGGCGGCGGCAGCTCAGAAACAGAAACTGCTTCTCCAGAGAGTGGAAACCGATATAGCCAACATTGTTGACAATTTCACTCACCTTGTCAACGTCTCTAGGGTAGCACTCTTTCCTTTTTCTTGTAATTCTCTTTGGTGGTCTATCTGCTTGATCTTCTTGATTTTATCACCACATGTTTGGAATTCATAATTCAAACTTAGTGTTCTTGTTTCCTCAGGTGAATGATCCACCTGTTAGAAACTCACAGGAATCTTTCATGATGGAAATGCGTGCTGCCAGAATGGTAAGATAAGAATTTTTCCCTTGTTTGTTTACTTACTGCGTTTGTGAAGCTCCTTTCAAGTTATCTTATGGATAGATAGCTCTCTCATTTGTTCTCCTATTGAATGATATTAATCTTATGAAGCCTCGATTTCTATGGATTTAATGCATGTGCGATAAAATCAGTTGCTTGTGCATCTCTTCCAGTAATTAAGGTGGTCTTCAAATTTGTAATATCCCTCTGGCGATCCCAATTGATTCTTCAGAAAAACTTAATTGAGCTACCTTAGAAATTCATTCAACATCAGTGCCTTGATATGGTGTTAATTCATGAGACAATGATTGCTGTTTTTGACCGAAAATTCATCAAATCATTATGGAGCTCTGATGAAGTGAGTTGGATTAATGTTGACTATATTGGGAGATCGGGTGGTTTACTGATTTTGTGGGGTGATATAAAGATAAAAGTCTTGGAGTTTCTTGAAGGAGGGTACACTTTCTCAATTAAAATCTATTTTTAGAACAAGTCAATTTGTTGGGTAACAAAGATTTATGGGCTAATTGACTATAGACTATAGAGAAAGAAAACATATTTGGAGTGAAATCCGTTCTTTATTCTCATATTGTGAAGAACCCTTGGTGCATTGTAGGGGATTTAAATATAACCCACTGGGTTTCATGAAAGATCACCATAAGGAAGACAAACCAAAGGTTGGGAGACGATTCATACCATCTTTGATCGATAGATTTTTTGTTTCATAGCAACGGGATGAGTTATTCTACAATTGAAGGGTATTAAGACGAGCTTGAATTTTTTGTCTGACCACTATCCTTACTTTCAGAGGCTGGATTCATTGTTTGGAGGCCACCCCCCATTAGATTTTGTAACAGTTGGATGCAAATGTCAGTTTGTGAGGTGATGAATCATCGTTCAAGCTTCTCTTCACGACCTTTCTTATGGTTGGGCTGGCTATGTACTAGCTTTGAAATTGAGAAATTTTCATAGATCGCATGTCGATGCATCTGTGGAATTCAGTTGGTGGTTAAATCTTTTTTGGAGAAAGTATTTTGGCCTGCTTTTACTAGTATTGGTTCTTGTTATCAGCTGCTGTGTTCGCTTTTCGAGGTTTATCTGGAGTTGAGAATTGGTTTGATGAGTCTTTTTTTGTTTCTTGGCTGTTCTCATTTGATTGTAATTGTTTTAAGATATTGTTCTGTTTTTTTTTCCTCTTTTGAGATTTTGTATCCTTTGAACGTTTTCCTTTTCATTATTTCAACGGAAGTTATTTCTTGTTCAAAATTTTGATTTTTGGTTTCAAACTATCAAGGCTTCTTATATTCATAATGATCATTCACATATAAGACTATAGGCAATAATATATCAGGGATTTATTATTTCTGGCCTCATCAGCTTGATAAAGACGATTGCTTTTCATTATTATTGTTATGGCTTCTACTTTAGTGTTATTCTACTTGTTTCTGAATAGGTTCAGGCAGCTGATTCTCTGCTGAAGTTGGTTTCAGAACTTAAGCAGACAGCAATCTTTTCTGGATTTGCTTCACTAAACGATCATGTGGAGCAAAGAATTGTGGAATTCAATCAGCAGGCACAGCAGACCGACCGTATCTTATCTAGAATTGGTGGAGAAGCTGCAGCCAGCCTGAAGGAGCTTGAATCTCATTATTATTCTTCAACACAGAGGACTAGTCAGAACAGCCTGACGTGATTTGTAAGTTGTGAAATAAGTAAATTAATGATGTGATATTGTTGTCTTTTAAACTCAATTGCTTGCTAACCTGTATAATTACACGTAATCGAACTCTTCTAGCCTAAGTTTTTAGCGTTTTTCTTTGGCTTTTATTGTTATGCCACGCTAGATAATGCATGAACTGTTGGATAATTTTGAGCATTTTATTGTATTTATTTTATTATTAGTAAAAGGTTTATATAAAGGAGAATAAGTGAGTCTCCCCTTCCTTCTTTTAAGAAAAAATTAAAGCAACATATAGTGCAATTAGGCCATTCTTATCTTTTTATTTGTCTTCTTTCTCGAGATGCGTATATATTTTCAGTAGACTTTTTTACACAGAAAAGAGAAACGAACCTTTTTTTCCCCATAAACATGGATTCCAAAAAGGTTCTTTCCTTGAGCATACAAGTCAATAAAAATTCTTAATATAGCTTAGCTGCACACAAACTCTCTTGAAGGGAGTTGTATCCTAGGTATAGTGTCGAATAACTAAAAACTCATAGAACCGTTGATGAATGTGCCAAATATTACACCCCATGCAAGTTTAGGAAACATATTATACTTGTACTCTGAACAATTTGAGAGGCACATTTTGGTCCCTATGTTTCAAATATAGATGTCCTACAAGCAAAAGAATCCTCCAAATTTTACTAGAATAGAGAAGGTGACAAATTTTTTAGAGATGAAACATTTTGGTGCCTACACTTAAAAAATGTCTATTTCATAGGTATACAATTGTTGAAATTGGTCTAGAATTAGGCACAGTGACAAAATTTAACTTTTCAAATTTATATAATCAACCCTTTAATTTAAAATCTTGACTTTCATCATATGTTTTTAAAATAAAAAAACCGAAATAAGTATATAACCTCTTCTTCATTCTGCCCTGATGAGAATCACACTCTAATAAAAATTTGATGGGAAAACATAACTTGTTCTATAACTTGTCATCCTAGATCAAGTTATCATCTTCTTTTGCAGTCTGGTAGTGATCAAAATCCAAAAGCATCACTTTGGCACTCAAACTAAACTTTCTTTTTTACTTCTCTTGCATGATTTTGTGCAGCTATTCTTTTCCAGCAGCTTGTCTAGCAGCCATTCCAGCTGCATTCAGAGTTCAAAATCTGAACCAATTGGTTGGTGGTCTTGGTGAGTTCATGCCCATGTGCCCTAATTTTCTTCATCCTGGTTGTGGCCTTCTGTGTCCTTATGAAGGCTGCCTTCGCCGGAGTTCTGATCAATGGCGTCAACCACCTTGAAGTTCTTGATAAGATTAAGGTTATGGCATCGACCACCTTGAGTTCCGATCGAAGGTGAATTTGTGGTGTAACTGATTTTTGAGCTCTAGAAGATGATATTTGCACAGCTTGCTTGATTGTGTAAATAGCACACTGATCAAAATTTTCGTTGTATTTGAATTTTTATAGACCTAGTTATTTCTTTTTATTTG

mRNA sequence

GGGGATTTGTATTTTTGGTGGGCTAGTTTGTTGTAAGAGCCCATTAAGCAGCCATATCCAAGCCCATCTCTATTATTGAATTCCAAAGATTGTCTAGGGTTGCATCTACCTCTGTGGTGCTTCTTTGTAACTCCGGCAACGCCCATAAGCTCCGACCATCATCAACGACACGACCGCAGCTTCGACGAACTATCAGACACTTTCTAGCGAGGATCCAAGTCGATATTGAGCTGATTCAATTCCCATTCTATTATTATGAACAAAGGCATTGGTGGTGGAACTGGCCCCACTGCAGCGGCTGCGGCGGCGGCAGCTCAGAAACAGAAACTGCTTCTCCAGAGAGTGGAAACCGATATAGCCAACATTGTTGACAATTTCACTCACCTTGTCAACGTCTCTAGGGTGAATGATCCACCTGTTAGAAACTCACAGGAATCTTTCATGATGGAAATGCGTGCTGCCAGAATGGTTCAGGCAGCTGATTCTCTGCTGAAGTTGGTTTCAGAACTTAAGCAGACAGCAATCTTTTCTGGATTTGCTTCACTAAACGATCATGTGGAGCAAAGAATTGTGGAATTCAATCAGCAGGCACAGCAGACCGACCGTATCTTATCTAGAATTGGTGGAGAAGCTGCAGCCAGCCTGAAGGAGCTTGAATCTCATTATTATTCTTCAACACAGAGGACTAGTCAGAACAGCCTGACCTATTCTTTTCCAGCAGCTTGTCTAGCAGCCATTCCAGCTGCATTCAGAGTTCAAAATCTGAACCAATTGGTTGGTGGTCTTGGTGAGTTCATGCCCATGTGCCCTAATTTTCTTCATCCTGGTTGTGGCCTTCTGTGTCCTTATGAAGGCTGCCTTCGCCGGAGTTCTGATCAATGGCGTCAACCACCTTGAAGTTCTTGATAAGATTAAGGTTATGGCATCGACCACCTTGAGTTCCGATCGAAGGTGAATTTGTGGTGTAACTGATTTTTGAGCTCTAGAAGATGATATTTGCACAGCTTGCTTGATTGTGTAAATAGCACACTGATCAAAATTTTCGTTGTATTTGAATTTTTATAGACCTAGTTATTTCTTTTTATTTG

Coding sequence (CDS)

ATGAACAAAGGCATTGGTGGTGGAACTGGCCCCACTGCAGCGGCTGCGGCGGCGGCAGCTCAGAAACAGAAACTGCTTCTCCAGAGAGTGGAAACCGATATAGCCAACATTGTTGACAATTTCACTCACCTTGTCAACGTCTCTAGGGTGAATGATCCACCTGTTAGAAACTCACAGGAATCTTTCATGATGGAAATGCGTGCTGCCAGAATGGTTCAGGCAGCTGATTCTCTGCTGAAGTTGGTTTCAGAACTTAAGCAGACAGCAATCTTTTCTGGATTTGCTTCACTAAACGATCATGTGGAGCAAAGAATTGTGGAATTCAATCAGCAGGCACAGCAGACCGACCGTATCTTATCTAGAATTGGTGGAGAAGCTGCAGCCAGCCTGAAGGAGCTTGAATCTCATTATTATTCTTCAACACAGAGGACTAGTCAGAACAGCCTGACCTATTCTTTTCCAGCAGCTTGTCTAGCAGCCATTCCAGCTGCATTCAGAGTTCAAAATCTGAACCAATTGGTTGGTGGTCTTGGTGAGTTCATGCCCATGTGCCCTAATTTTCTTCATCCTGGTTGTGGCCTTCTGTGTCCTTATGAAGGCTGCCTTCGCCGGAGTTCTGATCAATGGCGTCAACCACCTTGA

Protein sequence

MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRILSRIGGEAAASLKELESHYYSSTQRTSQNSLTYSFPAACLAAIPAAFRVQNLNQLVGGLGEFMPMCPNFLHPGCGLLCPYEGCLRRSSDQWRQPP

Homology

[Close]

BLAST of Lsi11G007800.1 vs. ExPASy Swiss-Prot
Match: Q8LCH5 (Mediator of RNA polymerase II transcription subunit 22b OS=Arabidopsis thaliana OX=3702 GN=MED22B PE=1 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 5.1e-55
Identity = 112/140 (80.00%), Postives = 131/140 (93.57%), Query Frame = 0


Query: 4   GIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFM 63
           G GGG+GPTAAAAAAA QKQK LLQRVETDI ++VDNFT +VNVSRV+DPPV+NSQE++M
Sbjct: 10  GSGGGSGPTAAAAAAALQKQKALLQRVETDITSVVDNFTQIVNVSRVSDPPVKNSQETYM 69

Query: 64  MEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRILSRIG 123
           MEMRA+RMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRI EF+Q+A++T+R+L+RI 
Sbjct: 70  MEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIEEFDQEAEKTNRLLARIA 129

Query: 124 GEAAASLKELESHYYSSTQR 144
            +A+A+LKELESHYYSS QR
Sbjct: 130 DDASANLKELESHYYSSAQR 149

[Close]

BLAST of Lsi11G007800.1 vs. ExPASy Swiss-Prot
Match: Q9SA42 (Mediator of RNA polymerase II transcription subunit 22a OS=Arabidopsis thaliana OX=3702 GN=MED22A PE=1 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 2.5e-54
Identity = 111/147 (75.51%), Postives = 135/147 (91.84%), Query Frame = 0


Query: 1   MNK--GIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNS 60
           MNK  G GGG+GPTAAAAAAA QKQK LLQRV+TDI ++VDNF  +VNV+RV+DPP++NS
Sbjct: 2   MNKGGGSGGGSGPTAAAAAAALQKQKALLQRVDTDITSVVDNFNQIVNVARVSDPPMKNS 61

Query: 61  QESFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRI 120
           QE++MMEMRA+R+VQAADSLLKLVSELKQTAIFSGFASLNDHVEQRI EF+Q+A++T+R+
Sbjct: 62  QEAYMMEMRASRLVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIAEFDQEAEKTNRL 121

Query: 121 LSRIGGEAAASLKELESHYYSSTQRTS 146
           L+RI  +A+ASLKELESHYYSS QR++
Sbjct: 122 LARIADDASASLKELESHYYSSAQRST 148

[Close]

BLAST of Lsi11G007800.1 vs. ExPASy Swiss-Prot
Match: Q54XB0 (Putative mediator of RNA polymerase II transcription subunit 22 OS=Dictyostelium discoideum OX=44689 GN=med22 PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.0e-14
Identity = 43/143 (30.07%), Postives = 88/143 (61.54%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MN G G  + P         + Q+   +++++ I  +++N+ +++ +S+VND P++N+ E
Sbjct: 1   MNTG-GNNSLPPQTRGEVLFRDQQFFQKQIDSKIMQLLENYHNIIKISKVND-PLKNASE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRILS 120
            + ME R + M+ A + LLK++ ELKQ  I + F+++ + V  + + F+++ ++T++ + 
Sbjct: 61  IYEMETRTSNMLNAGEGLLKIIEELKQNLILNDFSTMAEEVRIQNLVFHKENERTNKSIK 120

Query: 121 RIGGEAAASLKELESHYYSSTQR 144
            I  E + SLKELE  YY+S+ +
Sbjct: 121 LISEELSRSLKELEDEYYNSSYK 141

[Close]

BLAST of Lsi11G007800.1 vs. ExPASy Swiss-Prot
Match: Q62276 (Mediator of RNA polymerase II transcription subunit 22 OS=Mus musculus OX=10090 GN=Med22 PE=1 SV=2)

HSP 1 Score: 80.5 bits (197), Expect = 2.6e-14
Identity = 49/137 (35.77%), Postives = 81/137 (59.12%), Query Frame = 0


Query: 14  AAAAAAAQKQKLLLQ----RVETDIANIVDNFTHLVNVSRVNDP-----PVRNSQESFMM 73
           A   A  Q ++ LLQ    R++ DI +I+DNFT ++  +++ D        +  Q+++ M
Sbjct: 2   AQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEM 61

Query: 74  EMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRILSRIGG 133
            +RAA +V+A +SL+KLVS+LKQ  I + F S+N+ ++QR  +     ++ DR L  +  
Sbjct: 62  HVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRALQEECDRKLITLRD 121

Query: 134 EAAASLKELESHYYSST 142
           E +  L ELE  YYSS+
Sbjct: 122 EVSIDLYELEEEYYSSS 138

[Close]

BLAST of Lsi11G007800.1 vs. ExPASy Swiss-Prot
Match: A0JPN6 (Mediator of RNA polymerase II transcription subunit 22 OS=Rattus norvegicus OX=10116 GN=Med22 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.6e-14
Identity = 49/137 (35.77%), Postives = 81/137 (59.12%), Query Frame = 0


Query: 14  AAAAAAAQKQKLLLQ----RVETDIANIVDNFTHLVNVSRVNDP-----PVRNSQESFMM 73
           A   A  Q ++ LLQ    R++ DI +I+DNFT ++  +++ D        +  Q+++ M
Sbjct: 2   AQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEM 61

Query: 74  EMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRILSRIGG 133
            +RAA +V+A +SL+KLVS+LKQ  I + F S+N+ ++QR  +     ++ DR L  +  
Sbjct: 62  HVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRALQEECDRKLITLRD 121

Query: 134 EAAASLKELESHYYSST 142
           E +  L ELE  YYSS+
Sbjct: 122 EVSIDLYELEEEYYSSS 138

[Close]

BLAST of Lsi11G007800.1 vs. ExPASy TrEMBL
Match: A0A0A0M174 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G701970 PE=4 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 1.3e-69
Identity = 146/150 (97.33%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRILS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDR+LS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120

Query: 121 RIGGEAAASLKELESHYYSSTQRTSQNSLT 151
           RIG EAAA+LKELESHYYSSTQRT QNSLT
Sbjct: 121 RIGEEAAANLKELESHYYSSTQRTDQNSLT 150

[Close]

BLAST of Lsi11G007800.1 vs. ExPASy TrEMBL
Match: A0A1S3C993 (mediator of RNA polymerase II transcription subunit 22b-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498265 PE=4 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 8.4e-69
Identity = 144/150 (96.00%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRILS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQT+R+LS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTERLLS 120

Query: 121 RIGGEAAASLKELESHYYSSTQRTSQNSLT 151
           RIG EAAA+LKELESHYYSSTQRT QN+LT
Sbjct: 121 RIGEEAAANLKELESHYYSSTQRTDQNNLT 150

[Close]

BLAST of Lsi11G007800.1 vs. ExPASy TrEMBL
Match: A0A5A7TMC4 (Mediator of RNA polymerase II transcription subunit 22a-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G00280 PE=4 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 8.4e-69
Identity = 144/150 (96.00%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 48  MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 107

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRILS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQT+R+LS
Sbjct: 108 SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTERLLS 167

Query: 121 RIGGEAAASLKELESHYYSSTQRTSQNSLT 151
           RIG EAAA+LKELESHYYSSTQRT QN+LT
Sbjct: 168 RIGEEAAANLKELESHYYSSTQRTDQNNLT 197

[Close]

BLAST of Lsi11G007800.1 vs. ExPASy TrEMBL
Match: A0A1S3CAG5 (mediator of RNA polymerase II transcription subunit 22a-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498265 PE=4 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 8.4e-69
Identity = 144/150 (96.00%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 48  MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 107

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRILS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQT+R+LS
Sbjct: 108 SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTERLLS 167

Query: 121 RIGGEAAASLKELESHYYSSTQRTSQNSLT 151
           RIG EAAA+LKELESHYYSSTQRT QN+LT
Sbjct: 168 RIGEEAAANLKELESHYYSSTQRTDQNNLT 197

[Close]

BLAST of Lsi11G007800.1 vs. ExPASy TrEMBL
Match: A0A6J1EN95 (mediator of RNA polymerase II transcription subunit 22b-like OS=Cucurbita moschata OX=3662 GN=LOC111436153 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 7.1e-68
Identity = 142/150 (94.67%), Postives = 146/150 (97.33%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRILS 120
           SFMMEMRA+RMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQA+QTDRI+S
Sbjct: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRIIS 120

Query: 121 RIGGEAAASLKELESHYYSSTQRTSQNSLT 151
           +IG EAA  LKELESHYYSSTQRTSQN LT
Sbjct: 121 KIGEEAAVGLKELESHYYSSTQRTSQNRLT 150

[Close]

BLAST of Lsi11G007800.1 vs. NCBI nr
Match: XP_004145443.1 (mediator of RNA polymerase II transcription subunit 22b [Cucumis sativus] >KGN66872.1 hypothetical protein Csa_007213 [Cucumis sativus])

HSP 1 Score: 272.7 bits (696), Expect = 2.7e-69
Identity = 146/150 (97.33%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRILS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDR+LS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120

Query: 121 RIGGEAAASLKELESHYYSSTQRTSQNSLT 151
           RIG EAAA+LKELESHYYSSTQRT QNSLT
Sbjct: 121 RIGEEAAANLKELESHYYSSTQRTDQNSLT 150

[Close]

BLAST of Lsi11G007800.1 vs. NCBI nr
Match: XP_008459037.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 22a-like isoform X1 [Cucumis melo] >KAA0043196.1 mediator of RNA polymerase II transcription subunit 22a-like isoform X1 [Cucumis melo var. makuwa] >TYK12271.1 mediator of RNA polymerase II transcription subunit 22a-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 270.0 bits (689), Expect = 1.7e-68
Identity = 144/150 (96.00%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 48  MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 107

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRILS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQT+R+LS
Sbjct: 108 SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTERLLS 167

Query: 121 RIGGEAAASLKELESHYYSSTQRTSQNSLT 151
           RIG EAAA+LKELESHYYSSTQRT QN+LT
Sbjct: 168 RIGEEAAANLKELESHYYSSTQRTDQNNLT 197

[Close]

BLAST of Lsi11G007800.1 vs. NCBI nr
Match: XP_008459039.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like isoform X2 [Cucumis melo] >XP_008459040.1 PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like isoform X2 [Cucumis melo] >XP_008459041.1 PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like isoform X2 [Cucumis melo])

HSP 1 Score: 270.0 bits (689), Expect = 1.7e-68
Identity = 144/150 (96.00%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRILS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQT+R+LS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTERLLS 120

Query: 121 RIGGEAAASLKELESHYYSSTQRTSQNSLT 151
           RIG EAAA+LKELESHYYSSTQRT QN+LT
Sbjct: 121 RIGEEAAANLKELESHYYSSTQRTDQNNLT 150

[Close]

BLAST of Lsi11G007800.1 vs. NCBI nr
Match: KAG7029838.1 (Mediator of RNA polymerase II transcription subunit 22b [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 269.6 bits (688), Expect = 2.3e-68
Identity = 144/150 (96.00%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRILS 120
           SFMMEMRA+RMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQA+QTDRILS
Sbjct: 61  SFMMEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAEQTDRILS 120

Query: 121 RIGGEAAASLKELESHYYSSTQRTSQNSLT 151
           +IG EAA  LKELESHYYSSTQRTSQNSLT
Sbjct: 121 KIGEEAAVGLKELESHYYSSTQRTSQNSLT 150

[Close]

BLAST of Lsi11G007800.1 vs. NCBI nr
Match: XP_038891249.1 (mediator of RNA polymerase II transcription subunit 22b-like [Benincasa hispida] >XP_038891250.1 mediator of RNA polymerase II transcription subunit 22b-like [Benincasa hispida] >XP_038891251.1 mediator of RNA polymerase II transcription subunit 22b-like [Benincasa hispida] >XP_038891252.1 mediator of RNA polymerase II transcription subunit 22b-like [Benincasa hispida])

HSP 1 Score: 269.6 bits (688), Expect = 2.3e-68
Identity = 145/150 (96.67%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 1   MNKGIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60
           MNKGIGGG GPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE
Sbjct: 1   MNKGIGGGIGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQE 60

Query: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRILS 120
           SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDR+LS
Sbjct: 61  SFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRLLS 120

Query: 121 RIGGEAAASLKELESHYYSSTQRTSQNSLT 151
            IG EAAASLKELESHYYSSTQRTSQN+LT
Sbjct: 121 TIGEEAAASLKELESHYYSSTQRTSQNNLT 150

[Close]

BLAST of Lsi11G007800.1 vs. TAIR 10
Match: AT1G07950.1 (Surfeit locus protein 5 subunit 22 of Mediator complex )

HSP 1 Score: 215.7 bits (548), Expect = 3.6e-56
Identity = 112/140 (80.00%), Postives = 131/140 (93.57%), Query Frame = 0


Query: 4   GIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNSQESFM 63
           G GGG+GPTAAAAAAA QKQK LLQRVETDI ++VDNFT +VNVSRV+DPPV+NSQE++M
Sbjct: 10  GSGGGSGPTAAAAAAALQKQKALLQRVETDITSVVDNFTQIVNVSRVSDPPVKNSQETYM 69

Query: 64  MEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRILSRIG 123
           MEMRA+RMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRI EF+Q+A++T+R+L+RI 
Sbjct: 70  MEMRASRMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIEEFDQEAEKTNRLLARIA 129

Query: 124 GEAAASLKELESHYYSSTQR 144
            +A+A+LKELESHYYSS QR
Sbjct: 130 DDASANLKELESHYYSSAQR 149

[Close]

BLAST of Lsi11G007800.1 vs. TAIR 10
Match: AT1G16430.1 (Surfeit locus protein 5 subunit 22 of Mediator complex )

HSP 1 Score: 213.4 bits (542), Expect = 1.8e-55
Identity = 111/147 (75.51%), Postives = 135/147 (91.84%), Query Frame = 0


Query: 1   MNK--GIGGGTGPTAAAAAAAAQKQKLLLQRVETDIANIVDNFTHLVNVSRVNDPPVRNS 60
           MNK  G GGG+GPTAAAAAAA QKQK LLQRV+TDI ++VDNF  +VNV+RV+DPP++NS
Sbjct: 2   MNKGGGSGGGSGPTAAAAAAALQKQKALLQRVDTDITSVVDNFNQIVNVARVSDPPMKNS 61

Query: 61  QESFMMEMRAARMVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIVEFNQQAQQTDRI 120
           QE++MMEMRA+R+VQAADSLLKLVSELKQTAIFSGFASLNDHVEQRI EF+Q+A++T+R+
Sbjct: 62  QEAYMMEMRASRLVQAADSLLKLVSELKQTAIFSGFASLNDHVEQRIAEFDQEAEKTNRL 121

Query: 121 LSRIGGEAAASLKELESHYYSSTQRTS 146
           L+RI  +A+ASLKELESHYYSS QR++
Sbjct: 122 LARIADDASASLKELESHYYSSAQRST 148

The following BLAST results are available for this feature:

BLAST of Lsi11G007800.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LCH5	5.1e-55	80.00	Mediator of RNA polymerase II transcription subunit 22b OS=Arabidopsis thaliana ...	[more]
Q9SA42	2.5e-54	75.51	Mediator of RNA polymerase II transcription subunit 22a OS=Arabidopsis thaliana ...	[more]
Q54XB0	2.0e-14	30.07	Putative mediator of RNA polymerase II transcription subunit 22 OS=Dictyostelium...	[more]
Q62276	2.6e-14	35.77	Mediator of RNA polymerase II transcription subunit 22 OS=Mus musculus OX=10090 ...	[more]
A0JPN6	2.6e-14	35.77	Mediator of RNA polymerase II transcription subunit 22 OS=Rattus norvegicus OX=1...	[more]

BLAST of Lsi11G007800.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M174	1.3e-69	97.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G701970 PE=4 SV=1	[more]
A0A1S3C993	8.4e-69	96.00	mediator of RNA polymerase II transcription subunit 22b-like isoform X2 OS=Cucum...	[more]
A0A5A7TMC4	8.4e-69	96.00	Mediator of RNA polymerase II transcription subunit 22a-like isoform X1 OS=Cucum...	[more]
A0A1S3CAG5	8.4e-69	96.00	mediator of RNA polymerase II transcription subunit 22a-like isoform X1 OS=Cucum...	[more]
A0A6J1EN95	7.1e-68	94.67	mediator of RNA polymerase II transcription subunit 22b-like OS=Cucurbita moscha...	[more]

BLAST of Lsi11G007800.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145443.1	2.7e-69	97.33	mediator of RNA polymerase II transcription subunit 22b [Cucumis sativus] >KGN66...	[more]
XP_008459037.1	1.7e-68	96.00	PREDICTED: mediator of RNA polymerase II transcription subunit 22a-like isoform ...	[more]
XP_008459039.1	1.7e-68	96.00	PREDICTED: mediator of RNA polymerase II transcription subunit 22b-like isoform ...	[more]
KAG7029838.1	2.3e-68	96.00	Mediator of RNA polymerase II transcription subunit 22b [Cucurbita argyrosperma ...	[more]
XP_038891249.1	2.3e-68	96.67	mediator of RNA polymerase II transcription subunit 22b-like [Benincasa hispida]...	[more]

BLAST of Lsi11G007800.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G07950.1	3.6e-56	80.00	Surfeit locus protein 5 subunit 22 of Mediator complex	[more]
AT1G16430.1	1.8e-55	75.51	Surfeit locus protein 5 subunit 22 of Mediator complex	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009332	Mediator of RNA polymerase II transcription subunit 22	PFAM	PF06179	Med22	coord: 28..126 e-value: 4.0E-27 score: 94.5
IPR009332	Mediator of RNA polymerase II transcription subunit 22	PANTHER	PTHR12434	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 22	coord: 1..146

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi11G007800	Lsi11G007800	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi11G007800.1.exon.1	Lsi11G007800.1.exon.1	exon
Lsi11G007800.1.exon.2	Lsi11G007800.1.exon.2	exon
Lsi11G007800.1.exon.3	Lsi11G007800.1.exon.3	exon
Lsi11G007800.1.exon.4	Lsi11G007800.1.exon.4	exon
Lsi11G007800.1.exon.5	Lsi11G007800.1.exon.5	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi11G007800.1.five_prime_UTR.1	Lsi11G007800.1.five_prime_UTR.1	five_prime_UTR
Lsi11G007800.1.five_prime_UTR.2	Lsi11G007800.1.five_prime_UTR.2	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi11G007800.1.CDS.1	Lsi11G007800.1.CDS.1	CDS
Lsi11G007800.1.CDS.2	Lsi11G007800.1.CDS.2	CDS
Lsi11G007800.1.CDS.3	Lsi11G007800.1.CDS.3	CDS
Lsi11G007800.1.CDS.4	Lsi11G007800.1.CDS.4	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi11G007800.1.three_prime_UTR.1	Lsi11G007800.1.three_prime_UTR.1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi11G007800.1	Lsi11G007800.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
biological_process	GO:0048193	Golgi vesicle transport
cellular_component	GO:0016592	mediator complex
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0005794	Golgi apparatus
cellular_component	GO:0030008	TRAPP complex
molecular_function	GO:0003712	transcription coregulator activity