Lsi11G007230.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi11G007230.1 vs. ExPASy Swiss-Prot
Match: M4ISH0 (4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 2.1e-219
Identity = 378/532 (71.05%), Postives = 450/532 (84.59%), Query Frame = 0

Query: 9   QDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVDLAARCVAA 68
           ++FIFRSKLPDIYIPNHLPLH+YCFE++SQF+ RPCLING TGE+ TYA VDL +R VAA
Sbjct: 11  EEFIFRSKLPDIYIPNHLPLHSYCFENISQFKDRPCLINGATGEIITYADVDLTSRKVAA 70

Query: 69  GLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQAAAARPKV 128
           GL K+G+ QGDVIML+LQNSPEFV+AFL AS+ GAI TTANP Y P E+AKQAAA++ K+
Sbjct: 71  GLDKLGIKQGDVIMLLLQNSPEFVYAFLAASYIGAIITTANPFYTPAEVAKQAAASKTKL 130

Query: 129 IITQAEFVEKVWGF-AVEHGVKILCTDSPP--VGCLRFSEVMEADENEIPAVKINSNDVV 188
           +IT A +++KV  F   E GVK++C D+PP    CL FSE+ +ADE EIPAVKI+ +DVV
Sbjct: 131 VITLAGYIDKVKEFTGGESGVKVMCVDAPPPESECLHFSELTQADETEIPAVKIHPDDVV 190

Query: 189 ALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPLFHIYSLNSV 248
           ALP+SSGTTG+PKGVMLTHK LVTSVAQQVDG+NPN+  + NDVI+CVLPLFHIYSLNS+
Sbjct: 191 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHQNDVILCVLPLFHIYSLNSI 250

Query: 249 LLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSIRI 308
           LLCGLRVGA+ILI+QK+++S LL LI+  K TIAPFVPPIVL  AK PD+HRYDLSSIR 
Sbjct: 251 LLCGLRVGAAILIMQKFEISKLLELIEKFKVTIAPFVPPIVLSVAKCPDLHRYDLSSIRT 310

Query: 309 VMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGACGTVV 368
           VMSG APMGK+LED VK +LP+A LGQGYGMTEAGPVLSMCLAFAKEPF +KSGACGTVV
Sbjct: 311 VMSGGAPMGKELEDAVKEKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFPIKSGACGTVV 370

Query: 369 RNAEMKIIHPQTSVSLPRNH------------PGYLNNKEATEKTIDKDGWLHTGDLGFI 428
           RNAEMKI+ P T  SLPRN              GY+N+ EAT+ TID+ GWLHTGD+GFI
Sbjct: 371 RNAEMKIVDPDTGASLPRNQSGEICIRGKQIMKGYINDAEATKGTIDEGGWLHTGDIGFI 430

Query: 429 DDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGEIPVAFVV 488
           D+DDE+FIVDRLKELIKYKG+QVAPAELE++L S+P I+ AAV+PM+DEAAGE+PVAFVV
Sbjct: 431 DNDDELFIVDRLKELIKYKGFQVAPAELESMLISHPNITDAAVVPMKDEAAGEVPVAFVV 490

Query: 489 RSKGSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQL 526
           RS GSKI+E DIK+YIS QV++YK+I K FF++ IP  PSGKILRK+L+A+L
Sbjct: 491 RSNGSKITEEDIKQYISKQVVFYKRINKAFFIEEIPKNPSGKILRKILRAKL 542

BLAST of Lsi11G007230.1 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 744.2 bits (1920), Expect = 1.0e-213
Identity = 362/540 (67.04%), Postives = 446/540 (82.59%), Query Frame = 0

Query: 1   MEHELMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVD 60
           M+ E     D IFRSKLPDIYIP HLPLH+YCFE+LS+F  RPCLI+G    ++TYA V+
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LAARCVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQ 120
           L +R VA GL+K+G+ Q D IM++L N PEFVFAF+GAS+ GAIST ANPL+ P E+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AAAARPKVIITQAEFVEKVWGFAVEHGVKILCTDSPPVGCLRFSEVMEADENEIPAVKIN 180
           A A+  K++ITQA F  KV  +A+E+ +K++C DS P GC+ FSE++++DE+EIP VKI 
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPLFHIY 240
            +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ ++S+DV++CVLPLFHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSVLLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLC LRVGA+ILI+QK+D++  L LI  HK TI PFVPPIVL  AK+P +H YDL
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 302

Query: 301 SSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGA 360
           SS+R VMSGAAP+GK+LED V+A+ PNA LGQGYGMTEAGPVL+MCLAFAKEPF++KSGA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIIHPQTSVSLPRNHP------------GYLNNKEATEKTIDKDGWLHTG 420
           CGTVVRNAEMKI+ P T  SLPRN P            GYLN+ EAT +TI+K+GWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGEIP 480
           D+GFIDDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++P IS AAV+PM DE AGE+P
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFVVRSKGSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLEAG 529
           VAFVVRS GS I+E+++K +IS QVI+YK+I++VFFV+++P +PSGKILRK L+A+L AG
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

BLAST of Lsi11G007230.1 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 5.1e-213
Identity = 360/537 (67.04%), Postives = 444/537 (82.68%), Query Frame = 0

Query: 4   ELMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVDLAA 63
           E     D IFRSKLPDIYIP HLPLH+YCFE++S+F  RPCLING   +++TYA V+L  
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RCVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQAAA 123
           R VA GL+K+G+ Q D IM++L NSPEFVFAF+GAS+ GAIST ANPL+ P E+ KQA A
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 ARPKVIITQAEFVEKVWGFAVEHGVKILCTDSPPVGCLRFSEVMEADENEIPAVKINSND 183
           +  K+IITQ+ FV KV  +A E+ VK++C DS P GCL FSE+ ++DE+EIP VKI  +D
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDD 187

Query: 184 VVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPLFHIYSLN 243
           VVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ ++S DV++CVLPLFHIYSLN
Sbjct: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 247

Query: 244 SVLLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSI 303
           S+LLCGLRVGA+ILI+QK+D++  L LIQ +K +I PFVPPIVL  AK+P +  YDLSS+
Sbjct: 248 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 307

Query: 304 RIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGACGT 363
           R VMSGAAP+GK+LED V+ + PNA LGQGYGMTEAGPVL+MCLAFAKEPF++KSGACGT
Sbjct: 308 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 367

Query: 364 VVRNAEMKIIHPQTSVSLPRNHP------------GYLNNKEATEKTIDKDGWLHTGDLG 423
           VVRNAEMKI+ P T  SLPRN P            GYLN+ EAT +TIDK+GWLHTGD+G
Sbjct: 368 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 427

Query: 424 FIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGEIPVAF 483
           FID+DDE+FIVDRLKELIKYKG+QVAPAE+EALL ++P IS AAV+PM+DE AGE+PVAF
Sbjct: 428 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 487

Query: 484 VVRSKGSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLEAG 529
           VVRS GS I+E+++K +IS QVI+YK++++VFFV+++P +PSGKILRK L+A+L AG
Sbjct: 488 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAG 544

BLAST of Lsi11G007230.1 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 1.1e-212
Identity = 361/540 (66.85%), Postives = 445/540 (82.41%), Query Frame = 0

Query: 1   MEHELMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVD 60
           M+ E     D IFRSKLPDIYIP HLPLH+YCFE+LS+F  RPCLI+G    ++TYA V+
Sbjct: 3   MDTETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LAARCVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQ 120
           L +R VA GL+K+G+ Q D IM++L N PEFVFAF+GAS+ GAIST ANPL+ P E+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AAAARPKVIITQAEFVEKVWGFAVEHGVKILCTDSPPVGCLRFSEVMEADENEIPAVKIN 180
           A A+  K++ITQA F  KV  +A+E+ +K++C DS P GC+ FSE++++DE+EIP VKI 
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSVPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPLFHIY 240
            +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ ++S+DV++CVLPLFHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSVLLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLC LRVGA+ILI+QK+D++  L LI  HK TI PFVPPIVL  AK+P +  YDL
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDL 302

Query: 301 SSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGA 360
           SS+R VMSGAAP+GK+LED V+A+ PNA LGQGYGMTEAGPVL+MCLAFAKEPF++KSGA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIIHPQTSVSLPRNHP------------GYLNNKEATEKTIDKDGWLHTG 420
           CGTVVRNAEMKI+ P T  SLPRN P            GYLN+ EAT +TI+K+GWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGEIP 480
           D+GFIDDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++P IS AAV+PM DE AGE+P
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFVVRSKGSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLEAG 529
           VAFVVRS GS I+E+++K +IS QVI+YK+I++VFFV+++P +PSGKILRK L+A+L AG
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

BLAST of Lsi11G007230.1 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 1.9e-212
Identity = 366/531 (68.93%), Postives = 444/531 (83.62%), Query Frame = 0

Query: 10  DFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVDLAARCVAAG 69
           D IFRSKLPDIYIPNHLPLH+YCFE++S+F  RPCLING   +++TYA V+L +R VAAG
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 70  LSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQAAAARPKVI 129
           L K G+   D IM++L NSPEFVFAF+GAS+ GAIST ANPL+ P E+ KQA A+  K+I
Sbjct: 69  LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 130 ITQAEFVEKVWGFAVEHGVKILCTDSPPVGCLRFSEVMEADENEIPAVKINSNDVVALPF 189
           +TQA  V KV  +A E+ VKI+C DS P GCL FS + +A+E++IP V+I  +DVVALP+
Sbjct: 129 VTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVALPY 188

Query: 190 SSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPLFHIYSLNSVLLCG 249
           SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+ ++S DV++CVLPLFHIYSLNSVLLCG
Sbjct: 189 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCG 248

Query: 250 LRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSIRIVMSG 309
           LRVGA+ILI+QK+D+ S L LIQ +K TI PFVPPIVL  AK+P +  YDLSS+R VMSG
Sbjct: 249 LRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTVMSG 308

Query: 310 AAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGACGTVVRNAE 369
           AAP+GK+LEDTV+A+ PNA LGQGYGMTEAGPVL+MCLAFAKEPFE+KSGACGTVVRNAE
Sbjct: 309 AAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAE 368

Query: 370 MKIIHPQTSVSLPRNH------------PGYLNNKEATEKTIDKDGWLHTGDLGFIDDDD 429
           MKI+ P+T  SLPRN              GYLN+ EAT +TIDK+GWL+TGD+G+IDDDD
Sbjct: 369 MKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDDD 428

Query: 430 EIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGEIPVAFVVRSKG 489
           E+FIVDRLKELIKYKG+QVAPAELEALL ++P IS AAV+PM+DE AGE+PVAFVVRS G
Sbjct: 429 ELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNG 488

Query: 490 SKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLEAG 529
           S I+E+++K +IS QVI+YK+I++VFFVD+IP +PSGKILRK L+A+L AG
Sbjct: 489 STITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAG 539

BLAST of Lsi11G007230.1 vs. ExPASy TrEMBL
Match: A0A5D3CKT4 (4-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00190 PE=4 SV=1)

HSP 1 Score: 971.8 bits (2511), Expect = 1.1e-279
Identity = 489/534 (91.57%), Postives = 507/534 (94.94%), Query Frame = 0

Query: 1   MEHELMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVD 60
           M+HE  NGQDFIF+SKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTG +HTYA VD
Sbjct: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60

Query: 61  LAARCVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQ 120
           LAAR  AAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAI+TTANPLYKPGEI KQ
Sbjct: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120

Query: 121 AAAARPKVIITQAEFVEKVWGFAVEHGVKILCTD----SPPVGCLRFSEVMEADENEIPA 180
           A AAR KVIITQ EFVEKVW FAVE+GVKILCTD    S   GCLRFSEVMEADEN+IPA
Sbjct: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVN+NS DVIICVLPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGA+ILILQKYDMSSLLRLIQ HKATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 RYDLSSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEV 360
            Y+LSSIRIVMSGAAPMGKDLEDTVKARLPNA LGQGYGMTEAGPVLSMCL FAKEPF V
Sbjct: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360

Query: 361 KSGACGTVVRNAEMKIIHPQTSVSLPRNHPGYLNNKEATEKTIDKDGWLHTGDLGFIDDD 420
           KSGACGTVVRNAEMKIIHPQ+S+SLPRNHPGYLNN+EATEKTIDKDGWLHTGDLGFIDDD
Sbjct: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGYLNNEEATEKTIDKDGWLHTGDLGFIDDD 420

Query: 421 DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGEIPVAFVVRSK 480
           DEIFIVDRLKELIKYKGYQVAPAELEALLTSNP I++AAVIPMEDEAAGEIPVAFVVRSK
Sbjct: 421 DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVAFVVRSK 480

Query: 481 GSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLEAGAF 531
           GSKISENDIK YISDQVIYYK+IRKVFFVDSIP+APSGKILRKVLKAQLEAG F
Sbjct: 481 GSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQLEAGVF 534

BLAST of Lsi11G007230.1 vs. ExPASy TrEMBL
Match: A0A6J1EP35 (4-coumarate--CoA ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436262 PE=4 SV=1)

HSP 1 Score: 969.5 bits (2505), Expect = 5.5e-279
Identity = 488/542 (90.04%), Postives = 510/542 (94.10%), Query Frame = 0

Query: 1   MEHELMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVD 60
           M+ +L N QDFIFRSKLPDIYIPNHLPLHTYCFEHLSQF HRPCLIN  TG++HTYAAVD
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LAARCVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQ 120
           LAAR VA GLSKIGVGQGDVIML+LQNSPEFVFAFLGAS+AGAISTTANPLYKP EI KQ
Sbjct: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 AAAARPKVIITQAEFVEKVWGFAVEHGVKILCTDSPPVGCLRFSEVMEADENEIPAVKIN 180
           AA ARPKVIITQAEFVEKV  FAVE+GVKI+CTDSPPVGCLRFSE++EADENEIPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPLFHIY 240
           SNDVVALPFSSGTTGVPKGVMLTHKSLVTS+AQQVDGENPNV+LNSNDV+IC+LPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLCGLRVGA+ILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGA 360
           SSIRIVMSGAAPMGK LEDTVKARLPNATLGQGYGMTEAGPVLSMCL FAKEPFE KSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPQTSVSLPRNHP------------GYLNNKEATEKTIDKDGWLHTG 420
           CGTVVRNAEMKIIHP+T VSLPRNHP            GYLNNKEATEKTIDK+GWLHTG
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKGYLNNKEATEKTIDKNGWLHTG 420

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGEIP 480
           DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNP +S+AAV+PMEDEAAGEIP
Sbjct: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480

Query: 481 VAFVVRSKGSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLEAG 531
            AFVVRSKGSKISE+DIKKYISDQVIYYKKIRKVFFVDSIP+APSGKILRKVLKAQLE G
Sbjct: 481 AAFVVRSKGSKISEDDIKKYISDQVIYYKKIRKVFFVDSIPVAPSGKILRKVLKAQLENG 540

BLAST of Lsi11G007230.1 vs. ExPASy TrEMBL
Match: A0A6J1KB99 (4-coumarate--CoA ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111492331 PE=4 SV=1)

HSP 1 Score: 966.1 bits (2496), Expect = 6.1e-278
Identity = 485/542 (89.48%), Postives = 510/542 (94.10%), Query Frame = 0

Query: 1   MEHELMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVD 60
           M+ +L N QDFIFRSKLPDIYIPNHLPLHTYCFEHLSQF HRPCLIN  TG++HTYAAVD
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LAARCVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQ 120
           LA+R VA GLSKIGVGQGDVIML+LQNSPEFVFAFLGAS+AGAISTTANPLYKP EI KQ
Sbjct: 61  LASRRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 AAAARPKVIITQAEFVEKVWGFAVEHGVKILCTDSPPVGCLRFSEVMEADENEIPAVKIN 180
           AA ARPKVIITQAEFVEKV  FA+E+GVKI+CTDSPPVGCLRFSE++EADENEIPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFAMENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPLFHIY 240
           SNDVVALPFSSGTTGVPKGVMLTHKSLVTS+AQQVDGENPNV+LNSNDV+IC+LPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLCGLRVGA+ILILQKYDMS+LLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSTLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGA 360
           SSIRIVMSGAAPMGK LEDTVKARLPNATLGQGYGMTEAGPVLSMCL FAKEPFE KSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPQTSVSLPRNHP------------GYLNNKEATEKTIDKDGWLHTG 420
           CGTVVRNAEMKIIHP+T VSLPRNHP            GYLNNKEATEKTIDK+GWLHTG
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKGYLNNKEATEKTIDKNGWLHTG 420

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGEIP 480
           DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNP +S+AAV+PMEDEAAGEIP
Sbjct: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480

Query: 481 VAFVVRSKGSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLEAG 531
            AFVVRSKGSKISE+DIKKYISDQVIYYKKIRKVFFVDSIP+APSGKILRKVLKAQLE G
Sbjct: 481 AAFVVRSKGSKISEDDIKKYISDQVIYYKKIRKVFFVDSIPVAPSGKILRKVLKAQLENG 540

BLAST of Lsi11G007230.1 vs. ExPASy TrEMBL
Match: A0A5A7U0C7 (4-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1468G00210 PE=4 SV=1)

HSP 1 Score: 965.7 bits (2495), Expect = 8.0e-278
Identity = 489/539 (90.72%), Postives = 507/539 (94.06%), Query Frame = 0

Query: 1   MEHELMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVD 60
           M+HE  NGQDFIF+SKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTG +HTYA VD
Sbjct: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60

Query: 61  LAARCVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQ 120
           LAAR  AAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAI+TTANPLYKPGEI KQ
Sbjct: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120

Query: 121 AAAARPKVIITQAEFVEKVWGFAVEHGVKILCTD----SPPVGCLRFSEVMEADENEIPA 180
           A AAR KVIITQ EFVEKVW FAVE+GVKILCTD    S   GCLRFSEVMEADEN+IPA
Sbjct: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVN+NS DVIICVLPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGA+ILILQKYDMSSLLRLIQ HKATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 RYDLSSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEV 360
            Y+LSSIRIVMSGAAPMGKDLEDTVKARLPNA LGQGYGMTEAGPVLSMCL FAKEPF V
Sbjct: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360

Query: 361 KSGACGTVVRNAEMKIIHPQTSVSLPRNHPG-----YLNNKEATEKTIDKDGWLHTGDLG 420
           KSGACGTVVRNAEMKIIHPQ+S+SLPRNHPG     YLNN+EATEKTIDKDGWLHTGDLG
Sbjct: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRYLNNEEATEKTIDKDGWLHTGDLG 420

Query: 421 FIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGEIPVAF 480
           FIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNP I++AAVIPMEDEAAGEIPVAF
Sbjct: 421 FIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVAF 480

Query: 481 VVRSKGSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLEAGAF 531
           VVRSKGSKISENDIK YISDQVIYYK+IRKVFFVDSIP+APSGKILRKVLKAQLEAG F
Sbjct: 481 VVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQLEAGVF 539

BLAST of Lsi11G007230.1 vs. ExPASy TrEMBL
Match: A0A1S3BSW8 (4-coumarate--CoA ligase 2-like OS=Cucumis melo OX=3656 GN=LOC103493360 PE=4 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 5.2e-277
Identity = 489/546 (89.56%), Postives = 507/546 (92.86%), Query Frame = 0

Query: 1   MEHELMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVD 60
           M+HE  NGQDFIF+SKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTG +HTYA VD
Sbjct: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60

Query: 61  LAARCVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQ 120
           LAAR  AAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAI+TTANPLYKPGEI KQ
Sbjct: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120

Query: 121 AAAARPKVIITQAEFVEKVWGFAVEHGVKILCTD----SPPVGCLRFSEVMEADENEIPA 180
           A AAR KVIITQ EFVEKVW FAVE+GVKILCTD    S   GCLRFSEVMEADEN+IPA
Sbjct: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVN+NS DVIICVLPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGA+ILILQKYDMSSLLRLIQ HKATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 RYDLSSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEV 360
            Y+LSSIRIVMSGAAPMGKDLEDTVKARLPNA LGQGYGMTEAGPVLSMCL FAKEPF V
Sbjct: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360

Query: 361 KSGACGTVVRNAEMKIIHPQTSVSLPRNHP------------GYLNNKEATEKTIDKDGW 420
           KSGACGTVVRNAEMKIIHPQ+S+SLPRNHP            GYLNN+EATEKTIDKDGW
Sbjct: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW 420

Query: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAA 480
           LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNP I++AAVIPMEDEAA
Sbjct: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480

Query: 481 GEIPVAFVVRSKGSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQ 531
           GEIPVAFVVRSKGSKISENDIK YISDQVIYYK+IRKVFFVDSIP+APSGKILRKVLKAQ
Sbjct: 481 GEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQ 540

BLAST of Lsi11G007230.1 vs. NCBI nr
Match: XP_038890953.1 (4-coumarate--CoA ligase 2-like [Benincasa hispida])

HSP 1 Score: 1003.4 bits (2593), Expect = 7.1e-289
Identity = 509/542 (93.91%), Postives = 523/542 (96.49%), Query Frame = 0

Query: 1   MEHELMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVD 60
           M+H LMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLING TGEVHTYAAVD
Sbjct: 1   MDHGLMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGATGEVHTYAAVD 60

Query: 61  LAARCVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQ 120
           LAAR VAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQ
Sbjct: 61  LAARRVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQ 120

Query: 121 AAAARPKVIITQAEFVEKVWGFAVEHGVKILCTDSPPVGCLRFSEVMEADENEIPAVKIN 180
           AA ARPKVIITQAEFVEKVWG+AVEHGVKILCTDSPPVGCLRFSEVMEADEN+IPAVKIN
Sbjct: 121 AAGARPKVIITQAEFVEKVWGYAVEHGVKILCTDSPPVGCLRFSEVMEADENDIPAVKIN 180

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPLFHIY 240
           SNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNL SNDVIICVLPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLYSNDVIICVLPLFHIY 240

Query: 241 SLNSVLLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLCGLRVGA+ILIL+KYDMSSLL LIQT+KATIAPFVPPIVLDFAKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILKKYDMSSLLPLIQTYKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGA 360
           SSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGA
Sbjct: 301 SSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGA 360

Query: 361 CGTVVRNAEMKIIHPQTSVSLPRNHP------------GYLNNKEATEKTIDKDGWLHTG 420
           CGTVVRNAEMKII+P+TSVSLPRNHP            GY+NN+EATEKTIDKDGWLHTG
Sbjct: 361 CGTVVRNAEMKIINPETSVSLPRNHPGEICIRGSQIMKGYINNREATEKTIDKDGWLHTG 420

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGEIP 480
           DLGFIDD+DEIFIVDRLKELIKYKGYQVAPAELEALLTSNP IS+AAVIPMEDEAAGEIP
Sbjct: 421 DLGFIDDNDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNISSAAVIPMEDEAAGEIP 480

Query: 481 VAFVVRSKGSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLEAG 531
           VAFVVRSKGSKISE+D+KKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLEAG
Sbjct: 481 VAFVVRSKGSKISEDDLKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLEAG 540

BLAST of Lsi11G007230.1 vs. NCBI nr
Match: TYK10849.1 (4-coumarate--CoA ligase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 971.8 bits (2511), Expect = 2.3e-279
Identity = 489/534 (91.57%), Postives = 507/534 (94.94%), Query Frame = 0

Query: 1   MEHELMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVD 60
           M+HE  NGQDFIF+SKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTG +HTYA VD
Sbjct: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60

Query: 61  LAARCVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQ 120
           LAAR  AAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAI+TTANPLYKPGEI KQ
Sbjct: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120

Query: 121 AAAARPKVIITQAEFVEKVWGFAVEHGVKILCTD----SPPVGCLRFSEVMEADENEIPA 180
           A AAR KVIITQ EFVEKVW FAVE+GVKILCTD    S   GCLRFSEVMEADEN+IPA
Sbjct: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVN+NS DVIICVLPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGA+ILILQKYDMSSLLRLIQ HKATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 RYDLSSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEV 360
            Y+LSSIRIVMSGAAPMGKDLEDTVKARLPNA LGQGYGMTEAGPVLSMCL FAKEPF V
Sbjct: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360

Query: 361 KSGACGTVVRNAEMKIIHPQTSVSLPRNHPGYLNNKEATEKTIDKDGWLHTGDLGFIDDD 420
           KSGACGTVVRNAEMKIIHPQ+S+SLPRNHPGYLNN+EATEKTIDKDGWLHTGDLGFIDDD
Sbjct: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGYLNNEEATEKTIDKDGWLHTGDLGFIDDD 420

Query: 421 DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGEIPVAFVVRSK 480
           DEIFIVDRLKELIKYKGYQVAPAELEALLTSNP I++AAVIPMEDEAAGEIPVAFVVRSK
Sbjct: 421 DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVAFVVRSK 480

Query: 481 GSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLEAGAF 531
           GSKISENDIK YISDQVIYYK+IRKVFFVDSIP+APSGKILRKVLKAQLEAG F
Sbjct: 481 GSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQLEAGVF 534

BLAST of Lsi11G007230.1 vs. NCBI nr
Match: XP_022929776.1 (4-coumarate--CoA ligase 2-like [Cucurbita moschata])

HSP 1 Score: 969.5 bits (2505), Expect = 1.1e-278
Identity = 488/542 (90.04%), Postives = 510/542 (94.10%), Query Frame = 0

Query: 1   MEHELMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVD 60
           M+ +L N QDFIFRSKLPDIYIPNHLPLHTYCFEHLSQF HRPCLIN  TG++HTYAAVD
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LAARCVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQ 120
           LAAR VA GLSKIGVGQGDVIML+LQNSPEFVFAFLGAS+AGAISTTANPLYKP EI KQ
Sbjct: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 AAAARPKVIITQAEFVEKVWGFAVEHGVKILCTDSPPVGCLRFSEVMEADENEIPAVKIN 180
           AA ARPKVIITQAEFVEKV  FAVE+GVKI+CTDSPPVGCLRFSE++EADENEIPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPLFHIY 240
           SNDVVALPFSSGTTGVPKGVMLTHKSLVTS+AQQVDGENPNV+LNSNDV+IC+LPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLCGLRVGA+ILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGA 360
           SSIRIVMSGAAPMGK LEDTVKARLPNATLGQGYGMTEAGPVLSMCL FAKEPFE KSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPQTSVSLPRNHP------------GYLNNKEATEKTIDKDGWLHTG 420
           CGTVVRNAEMKIIHP+T VSLPRNHP            GYLNNKEATEKTIDK+GWLHTG
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKGYLNNKEATEKTIDKNGWLHTG 420

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGEIP 480
           DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNP +S+AAV+PMEDEAAGEIP
Sbjct: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480

Query: 481 VAFVVRSKGSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLEAG 531
            AFVVRSKGSKISE+DIKKYISDQVIYYKKIRKVFFVDSIP+APSGKILRKVLKAQLE G
Sbjct: 481 AAFVVRSKGSKISEDDIKKYISDQVIYYKKIRKVFFVDSIPVAPSGKILRKVLKAQLENG 540

BLAST of Lsi11G007230.1 vs. NCBI nr
Match: XP_022997404.1 (4-coumarate--CoA ligase 2-like [Cucurbita maxima])

HSP 1 Score: 966.1 bits (2496), Expect = 1.3e-277
Identity = 485/542 (89.48%), Postives = 510/542 (94.10%), Query Frame = 0

Query: 1   MEHELMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVD 60
           M+ +L N QDFIFRSKLPDIYIPNHLPLHTYCFEHLSQF HRPCLIN  TG++HTYAAVD
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LAARCVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQ 120
           LA+R VA GLSKIGVGQGDVIML+LQNSPEFVFAFLGAS+AGAISTTANPLYKP EI KQ
Sbjct: 61  LASRRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 AAAARPKVIITQAEFVEKVWGFAVEHGVKILCTDSPPVGCLRFSEVMEADENEIPAVKIN 180
           AA ARPKVIITQAEFVEKV  FA+E+GVKI+CTDSPPVGCLRFSE++EADENEIPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFAMENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPLFHIY 240
           SNDVVALPFSSGTTGVPKGVMLTHKSLVTS+AQQVDGENPNV+LNSNDV+IC+LPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLCGLRVGA+ILILQKYDMS+LLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSTLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGA 360
           SSIRIVMSGAAPMGK LEDTVKARLPNATLGQGYGMTEAGPVLSMCL FAKEPFE KSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPQTSVSLPRNHP------------GYLNNKEATEKTIDKDGWLHTG 420
           CGTVVRNAEMKIIHP+T VSLPRNHP            GYLNNKEATEKTIDK+GWLHTG
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKGYLNNKEATEKTIDKNGWLHTG 420

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGEIP 480
           DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNP +S+AAV+PMEDEAAGEIP
Sbjct: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480

Query: 481 VAFVVRSKGSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLEAG 531
            AFVVRSKGSKISE+DIKKYISDQVIYYKKIRKVFFVDSIP+APSGKILRKVLKAQLE G
Sbjct: 481 AAFVVRSKGSKISEDDIKKYISDQVIYYKKIRKVFFVDSIPVAPSGKILRKVLKAQLENG 540

BLAST of Lsi11G007230.1 vs. NCBI nr
Match: KAA0048648.1 (4-coumarate--CoA ligase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 965.7 bits (2495), Expect = 1.6e-277
Identity = 489/539 (90.72%), Postives = 507/539 (94.06%), Query Frame = 0

Query: 1   MEHELMNGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVD 60
           M+HE  NGQDFIF+SKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTG +HTYA VD
Sbjct: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60

Query: 61  LAARCVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQ 120
           LAAR  AAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAI+TTANPLYKPGEI KQ
Sbjct: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120

Query: 121 AAAARPKVIITQAEFVEKVWGFAVEHGVKILCTD----SPPVGCLRFSEVMEADENEIPA 180
           A AAR KVIITQ EFVEKVW FAVE+GVKILCTD    S   GCLRFSEVMEADEN+IPA
Sbjct: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVN+NS DVIICVLPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGA+ILILQKYDMSSLLRLIQ HKATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 RYDLSSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEV 360
            Y+LSSIRIVMSGAAPMGKDLEDTVKARLPNA LGQGYGMTEAGPVLSMCL FAKEPF V
Sbjct: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360

Query: 361 KSGACGTVVRNAEMKIIHPQTSVSLPRNHPG-----YLNNKEATEKTIDKDGWLHTGDLG 420
           KSGACGTVVRNAEMKIIHPQ+S+SLPRNHPG     YLNN+EATEKTIDKDGWLHTGDLG
Sbjct: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRYLNNEEATEKTIDKDGWLHTGDLG 420

Query: 421 FIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGEIPVAF 480
           FIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNP I++AAVIPMEDEAAGEIPVAF
Sbjct: 421 FIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVAF 480

Query: 481 VVRSKGSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLEAGAF 531
           VVRSKGSKISENDIK YISDQVIYYK+IRKVFFVDSIP+APSGKILRKVLKAQLEAG F
Sbjct: 481 VVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKAQLEAGVF 539

BLAST of Lsi11G007230.1 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 679.9 bits (1753), Expect = 1.7e-195
Identity = 342/542 (63.10%), Postives = 424/542 (78.23%), Query Frame = 0

Query: 7   NGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVDLAARCV 66
           N  D IFRSKLPDIYIPNHL LH Y F+++S+F  +PCLING TG V+TY+ V + +R +
Sbjct: 19  NNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQI 78

Query: 67  AAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQAAAARP 126
           AA   K+GV Q DV+ML+L N PEFV +FL ASF GA +T ANP + P EIAKQA A+  
Sbjct: 79  AANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNT 138

Query: 127 KVIITQAEFVEKVWGFAVEHGVKILCTDSP-----PVGCLRFSEVMEAD---ENEIPAVK 186
           K+IIT+A +V+K+     + GV I+C D       P GCLRF+E+ ++       I +V+
Sbjct: 139 KLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 198

Query: 187 INSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPLFH 246
           I+ +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  +S+DVI+CVLP+FH
Sbjct: 199 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 258

Query: 247 IYSLNSVLLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRY 306
           IY+LNS++LCGLRVGA+ILI+ K++++ LL LIQ  K T+AP VPPIVL  AK+ +  +Y
Sbjct: 259 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 318

Query: 307 DLSSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKS 366
           DLSSIR+V SGAAP+GK+LED V A+ PNA LGQGYGMTEAGPVL+M L FAKEPF VKS
Sbjct: 319 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 378

Query: 367 GACGTVVRNAEMKIIHPQTSVSLPRNHP------------GYLNNKEATEKTIDKDGWLH 426
           GACGTVVRNAEMKI+ P T  SL RN P            GYLNN  AT +TIDKDGWLH
Sbjct: 379 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 438

Query: 427 TGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGE 486
           TGD+G IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL  +P I+  AV+ M++EAAGE
Sbjct: 439 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGE 498

Query: 487 IPVAFVVRSKGSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLE 529
           +PVAFVV+SK S++SE+D+K+++S QV++YK+I KVFF +SIP APSGKILRK L+A+L 
Sbjct: 499 VPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLA 558

BLAST of Lsi11G007230.1 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 676.8 bits (1745), Expect = 1.4e-194
Identity = 343/535 (64.11%), Postives = 423/535 (79.07%), Query Frame = 0

Query: 10  DFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVDLAARCVAAG 69
           D IFRS+LPDIYIPNHLPLH Y FE++S+F  +PCLING TGEV+TYA V + +R +AAG
Sbjct: 20  DVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAAG 79

Query: 70  LSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQAAAARPKVI 129
           L  +GV Q DV+M++L NSPE V  FL ASF GAI+T+ANP + P EI+KQA A+  K+I
Sbjct: 80  LHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKLI 139

Query: 130 ITQAEFVEKVWGFAVEHGVKILCTDSP--PVGCLRFSEVMEADENEIPAV--KINSNDVV 189
           +TQ+ +V+K+       GV I+ TDS   P  CLRFSE+ +++E  + ++  KI+  DVV
Sbjct: 140 VTQSRYVDKIKNLQ-NDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDVV 199

Query: 190 ALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPLFHIYSLNSV 249
           ALPFSSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  N +DVI+CVLP+FHIY+LNS+
Sbjct: 200 ALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNSI 259

Query: 250 LLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSIRI 309
           +LC LRVGA+ILI+ K++++ LL  IQ  K T+A  VPPIVL  AK+P+  +YDLSS+R+
Sbjct: 260 MLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVRM 319

Query: 310 VMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGACGTVV 369
           V SGAAP+GK+LED + A+ PNA LGQGYGMTEAGPVL+M L FAKEPF VKSGACGTVV
Sbjct: 320 VKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVV 379

Query: 370 RNAEMKIIHPQTSVSLPRNHP------------GYLNNKEATEKTIDKDGWLHTGDLGFI 429
           RNAEMKI+ P T  SLPRN P            GYLN+  AT  TIDKDGWLHTGD+GFI
Sbjct: 380 RNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFI 439

Query: 430 DDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGEIPVAFVV 489
           DDDDE+FIVDRLKELIKYKG+QVAPAELE+LL  +P I+  AV+ M++E AGE+PVAFVV
Sbjct: 440 DDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVV 499

Query: 490 RSKGSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQLEAG 529
           RSK S ISE++IK+++S QV++YK+I KVFF DSIP APSGKILRK L+A+L  G
Sbjct: 500 RSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANG 553

BLAST of Lsi11G007230.1 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 637.1 bits (1642), Expect = 1.3e-182
Identity = 319/529 (60.30%), Postives = 408/529 (77.13%), Query Frame = 0

Query: 12  IFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVDLAARCVAAGLS 71
           IFRSKLPDI IPNHLPLHTYCFE LS    +PCLI G+TG+ +TY    L  R VA+GL 
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  KIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQAAAARPKVIIT 131
           K+G+ +GDVIM++LQNS EFVF+F+GAS  GA+STTANP Y   E+ KQ  ++  K+IIT
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 QAEFVEKVWGFAVEHGVKILCTDSP-PVGCLRFSEVMEADENE--IPAVKINSNDVVALP 191
            +++V+K+        + ++ TD P P  CL FS ++  DE       V I  +D  ALP
Sbjct: 154 HSQYVDKLKNLG--ENLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALP 213

Query: 192 FSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPLFHIYSLNSVLLC 251
           FSSGTTG+PKGV+LTHKSL+TSVAQQVDG+NPN+ L SNDVI+CVLPLFHIYSLNSVLL 
Sbjct: 214 FSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLN 273

Query: 252 GLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSIRIVMS 311
            LR GA++L++ K+++ +LL LIQ H+ TIA  VPP+V+  AKNP ++ YDLSS+R V+S
Sbjct: 274 SLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLS 333

Query: 312 GAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGACGTVVRNA 371
           GAAP+GK+L+D+++ RLP A LGQGYGMTEAGPVLSM L FAKEP   KSG+CGTVVRNA
Sbjct: 334 GAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNA 393

Query: 372 EMKIIHPQTSVSLPRNHPG------------YLNNKEATEKTIDKDGWLHTGDLGFIDDD 431
           E+K++H +T +SL  N PG            YLN+ EAT  TID++GWLHTGD+G++D+D
Sbjct: 394 ELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDED 453

Query: 432 DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGEIPVAFVVRSK 491
           DEIFIVDRLKE+IK+KG+QV PAELE+LL ++  I+ AAV+P  DE AGE+PVAFVVRS 
Sbjct: 454 DEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSN 513

Query: 492 GSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQL 526
           G+ I+E D+K+Y++ QV++YK++ KVFFV SIP +PSGKILRK LKA+L
Sbjct: 514 GNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of Lsi11G007230.1 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 634.4 bits (1635), Expect = 8.1e-182
Identity = 319/507 (62.92%), Postives = 395/507 (77.91%), Query Frame = 0

Query: 7   NGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGEVHTYAAVDLAARCV 66
           N  D IFRSKLPDIYIPNHL LH Y F+++S+F  +PCLING TG V+TY+ V + +R +
Sbjct: 19  NNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQI 78

Query: 67  AAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQAAAARP 126
           AA   K+GV Q DV+ML+L N PEFV +FL ASF GA +T ANP + P EIAKQA A+  
Sbjct: 79  AANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNT 138

Query: 127 KVIITQAEFVEKVWGFAVEHGVKILCTDSP-----PVGCLRFSEVMEAD---ENEIPAVK 186
           K+IIT+A +V+K+     + GV I+C D       P GCLRF+E+ ++       I +V+
Sbjct: 139 KLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 198

Query: 187 INSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPLFH 246
           I+ +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  +S+DVI+CVLP+FH
Sbjct: 199 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 258

Query: 247 IYSLNSVLLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRY 306
           IY+LNS++LCGLRVGA+ILI+ K++++ LL LIQ  K T+AP VPPIVL  AK+ +  +Y
Sbjct: 259 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 318

Query: 307 DLSSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEVKS 366
           DLSSIR+V SGAAP+GK+LED V A+ PNA LGQGYGMTEAGPVL+M L FAKEPF VKS
Sbjct: 319 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 378

Query: 367 GACGTVVRNAEMKIIHPQTSVSLPRNHP------------GYLNNKEATEKTIDKDGWLH 426
           GACGTVVRNAEMKI+ P T  SL RN P            GYLNN  AT +TIDKDGWLH
Sbjct: 379 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 438

Query: 427 TGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAAGE 486
           TGD+G IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL  +P I+  AV+ M++EAAGE
Sbjct: 439 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGE 498

Query: 487 IPVAFVVRSKGSKISENDIKKYISDQV 494
           +PVAFVV+SK S++SE+D+K+++S QV
Sbjct: 499 VPVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of Lsi11G007230.1 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 608.6 bits (1568), Expect = 4.8e-174
Identity = 304/542 (56.09%), Postives = 403/542 (74.35%), Query Frame = 0

Query: 10  DFIFRSKLPDIYIPNHLPLHTYCFEHLS----QFQHRPCLINGTTGEVHTYAAVDLAARC 69
           DFIFRSKLPDI+IPNHLPL  Y F+  S          C+I+G TG + TYA V    R 
Sbjct: 26  DFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQTNMRR 85

Query: 70  VAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAKQAAAAR 129
           +AAG+ ++G+  GDV+ML+L NSPEF  +FL  ++ GA+STTANP Y   EIAKQA A+ 
Sbjct: 86  IAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQAKASA 145

Query: 130 PKVIITQAEFVEKVWGFAVEHGVKILCTD---------SPPVGCLRFSEVMEADENEIPA 189
            K+IIT+   V+K+       GV I+C D         S   GC+ F+E+ +ADE E+  
Sbjct: 146 AKMIITKKCLVDKLTNLK-NDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADETELLK 205

Query: 190 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLNSNDVIICVLPL 249
            KI+  D VA+P+SSGTTG+PKGVM+THK LVTS+AQ+VDGENPN+N  +NDVI+C LP+
Sbjct: 206 PKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCFLPM 265

Query: 250 FHIYSLNSVLLCGLRVGASILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIH 309
           FHIY+L++++L  +R GA++LI+ +++++ ++ LIQ +K T+ P  PP+VL F K+P+  
Sbjct: 266 FHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIKSPETE 325

Query: 310 RYDLSSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFEV 369
           RYDLSS+RI++SGAA + K+LED V+ + PNA  GQGYGMTE+G V +  LAFAK PF+ 
Sbjct: 326 RYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAKNPFKT 385

Query: 370 KSGACGTVVRNAEMKIIHPQTSVSLPRNH------------PGYLNNKEATEKTIDKDGW 429
           KSGACGTV+RNAEMK++  +T +SLPRN              GYLN+ EAT +TIDKDGW
Sbjct: 386 KSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTIDKDGW 445

Query: 430 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPYISAAAVIPMEDEAA 489
           LHTGD+GF+DDDDEIFIVDRLKELIK+KGYQVAPAELEALL S+P I  AAV+ M+DE A
Sbjct: 446 LHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMKDEVA 505

Query: 490 GEIPVAFVVRSKGSKISENDIKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKAQ 527
            E+PVAFV RS+GS+++E+D+K Y++ QV++YK+I+ VFF++ IP A SGKILRK L+A+
Sbjct: 506 DEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRKDLRAK 565