Lsi10G002860.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi10G002860.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Gamma-glutamylcyclotransferase family protein
Location	chr10: 4594496 .. 4595023 (+)
Sequence length	528
RNA-Seq Expression	Lsi10G002860.1
Synteny	Lsi10G002860.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGCCGAAGCCAATCAGACGATTCCGACGACCTTGATCTTCACCTACGGCACTCTGAAACGCGGTTTCTGGAACCACACGCTGTTGCAGGACCTGATGCGGACAGGAGACGCTTCTTTCATCGGAAGTTACCGGACGGCGAAGAGATTGCCGCTCGTCTGTGGACCGTTCCGAGTGCCGTTTCTGATAAACTCGCCCGGTTCTGGTCACCGTGTTGAAGGCGAGGTTTATGCCGTGACAAACAGAGGATTAGGGCGTACGGATGAACTGGAAGGCTTGAGCCGCGGCCACTACGTCCGGCTGCCGATTGATGTGGAGGCGACGGACGGCGGAGGCGTGGTAGCGGCGGAGGCGTATTATGCGCATCCAAGTTACGCGATGGAGCTGTGGAAGAAAGAGGGCGGCGGAGTGATAAGTTCGTACTCGGAGAAAGAGGCGAAAGGCTATGTGAAGCGTAAAGATAGGCCTCAACATATTTCGTTCCTCGATCAAATTCGTCTGTTTCTTTCTTCTTCGTCGGATTAG

mRNA sequence

ATGGTTGCCGAAGCCAATCAGACGATTCCGACGACCTTGATCTTCACCTACGGCACTCTGAAACGCGGTTTCTGGAACCACACGCTGTTGCAGGACCTGATGCGGACAGGAGACGCTTCTTTCATCGGAAGTTACCGGACGGCGAAGAGATTGCCGCTCGTCTGTGGACCGTTCCGAGTGCCGTTTCTGATAAACTCGCCCGGTTCTGGTCACCGTGTTGAAGGCGAGGTTTATGCCGTGACAAACAGAGGATTAGGGCGTACGGATGAACTGGAAGGCTTGAGCCGCGGCCACTACGTCCGGCTGCCGATTGATGTGGAGGCGACGGACGGCGGAGGCGTGGTAGCGGCGGAGGCGTATTATGCGCATCCAAGTTACGCGATGGAGCTGTGGAAGAAAGAGGGCGGCGGAGTGATAAGTTCGTACTCGGAGAAAGAGGCGAAAGGCTATGTGAAGCGTAAAGATAGGCCTCAACATATTTCGTTCCTCGATCAAATTCGTCTGTTTCTTTCTTCTTCGTCGGATTAG

Coding sequence (CDS)

ATGGTTGCCGAAGCCAATCAGACGATTCCGACGACCTTGATCTTCACCTACGGCACTCTGAAACGCGGTTTCTGGAACCACACGCTGTTGCAGGACCTGATGCGGACAGGAGACGCTTCTTTCATCGGAAGTTACCGGACGGCGAAGAGATTGCCGCTCGTCTGTGGACCGTTCCGAGTGCCGTTTCTGATAAACTCGCCCGGTTCTGGTCACCGTGTTGAAGGCGAGGTTTATGCCGTGACAAACAGAGGATTAGGGCGTACGGATGAACTGGAAGGCTTGAGCCGCGGCCACTACGTCCGGCTGCCGATTGATGTGGAGGCGACGGACGGCGGAGGCGTGGTAGCGGCGGAGGCGTATTATGCGCATCCAAGTTACGCGATGGAGCTGTGGAAGAAAGAGGGCGGCGGAGTGATAAGTTCGTACTCGGAGAAAGAGGCGAAAGGCTATGTGAAGCGTAAAGATAGGCCTCAACATATTTCGTTCCTCGATCAAATTCGTCTGTTTCTTTCTTCTTCGTCGGATTAG

Protein sequence

MVAEANQTIPTTLIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLVCGPFRVPFLINSPGSGHRVEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDVEATDGGGVVAAEAYYAHPSYAMELWKKEGGGVISSYSEKEAKGYVKRKDRPQHISFLDQIRLFLSSSSD

Homology

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BLAST of Lsi10G002860.1 vs. ExPASy Swiss-Prot
Match: Q9M8T3 (Putative gamma-glutamylcyclotransferase At3g02910 OS=Arabidopsis thaliana OX=3702 GN=At3g02910 PE=2 SV=2)

HSP 1 Score: 221.1 bits (562), Expect = 9.9e-57
Identity = 105/177 (59.32%), Postives = 128/177 (72.32%), Query Frame = 0


Query: 11  TTLIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLVCGPFRVPFLINSPGSG 70
           TTL+FTYGTLKRGF NH L+QDL+R+GDASF G Y+T  + PLVCGP+RVPFL+N PGSG
Sbjct: 11  TTLVFTYGTLKRGFSNHVLMQDLIRSGDASFKGVYQTLDKYPLVCGPYRVPFLLNKPGSG 70

Query: 71  HRVEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDVEA------------TDGGGVVAAE 130
           + V GE+YAV+ RGL R DELEG+SRGHY+R PI + A            T+       E
Sbjct: 71  YHVNGELYAVSPRGLSRLDELEGISRGHYIRQPIRLAAAEEEEEEEGDLETEAPSSCVVE 130

Query: 131 AYYAHPSYAMELWKKEGGGVISSYSEKEAKGYVKRKDRPQHISFLDQIRLFLSSSSD 176
           AYYAH SY  ELW++  G    +Y+E EA+GYVKR DRPQH+SFLD IR+F+SS  D
Sbjct: 131 AYYAHKSYEEELWRRNRGRSFGAYTENEARGYVKRNDRPQHLSFLDHIRIFVSSPCD 187

[Close]

BLAST of Lsi10G002860.1 vs. ExPASy Swiss-Prot
Match: Q66I06 (Gamma-glutamylaminecyclotransferase B OS=Danio rerio OX=7955 GN=ggact.2 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.2e-11
Identity = 45/112 (40.18%), Postives = 57/112 (50.89%), Query Frame = 0


Query: 13  LIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLV-CGPFRVPFLINSPGSGH 72
           L+F YGTLK+G  N+  L D    G A FI   RT +  PLV  G   +PFL+N PGSG 
Sbjct: 3   LVFVYGTLKKGQPNYFRLID-SSNGQAEFITCARTVEPYPLVITGECNIPFLLNVPGSGQ 62

Query: 73  RVEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDVEATDGGGVVAAEAYYAH 124
           RV GE+Y+V  + L   D  E      Y R  I +E   G G    E  + +
Sbjct: 63  RVYGEIYSVDQKMLEFLDWFEECP-DWYQRTLIQLEILKGNGETEVEEAFVY 112

[Close]

BLAST of Lsi10G002860.1 vs. ExPASy Swiss-Prot
Match: Q9BVM4 (Gamma-glutamylaminecyclotransferase OS=Homo sapiens OX=9606 GN=GGACT PE=1 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 2.2e-11
Identity = 40/81 (49.38%), Postives = 50/81 (61.73%), Query Frame = 0


Query: 13 LIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLV-CGPFRVPFLINSPGSGH 72
          L+F YGTLKRG  NH +L+D    G A+F    RT +  PLV  G   +P+L++ PGSG 
Sbjct: 3  LVFVYGTLKRGQPNHRVLRDGAH-GSAAFRARGRTLEPYPLVIAGEHNIPWLLHLPGSGR 62

Query: 73 RVEGEVYAVTNRGLGRTDELE 93
           VEGEVYAV  R L   D+ E
Sbjct: 63 LVEGEVYAVDERMLRFLDDFE 82

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BLAST of Lsi10G002860.1 vs. ExPASy Swiss-Prot
Match: Q9W0Y2 (Putative gamma-glutamylcyclotransferase CG2811 OS=Drosophila melanogaster OX=7227 GN=CG2811 PE=2 SV=2)

HSP 1 Score: 68.9 bits (167), Expect = 6.3e-11
Identity = 36/80 (45.00%), Postives = 46/80 (57.50%), Query Frame = 0


Query: 14 IFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLVCGP-FRVPFLINSPGSGHR 73
          +F YGTLKRG  NH  L      G A F+G  +T  + PLV G  + +PFL+  PG G+ 
Sbjct: 11 VFVYGTLKRGEPNHHWLTK-KENGQARFLGRGKTETKFPLVVGTRYNIPFLLARPGEGNH 70

Query: 74 VEGEVYAVTNRGLGRTDELE 93
          +EGEVY V    L + D LE
Sbjct: 71 IEGEVYEVDETMLSKLDILE 89

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BLAST of Lsi10G002860.1 vs. ExPASy Swiss-Prot
Match: Q4KM86 (Gamma-glutamylaminecyclotransferase OS=Rattus norvegicus OX=10116 GN=Ggact PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.1e-10
Identity = 46/122 (37.70%), Postives = 60/122 (49.18%), Query Frame = 0


Query: 14  IFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLV-CGPFRVPFLINSPGSGHR 73
           IF YGTLKRG  NH ++ D    G A+F G   T +  PLV  G   +P+L++ PG GH 
Sbjct: 4   IFVYGTLKRGQPNHKVMLD-QSHGLATFRGRGCTVESFPLVIAGEHNIPWLLHLPGKGHC 63

Query: 74  VEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDV-----EATDGGGVVAAEAYYAHPSYA 130
           V GE+Y V  + L   D+ EG     Y R  + V     E   G G       Y+  +YA
Sbjct: 64  VAGEIYEVDEQMLRFLDDFEGCP-SMYQRTALQVRVLEWEGAGGPGDSVQCFVYSTATYA 123

[Close]

BLAST of Lsi10G002860.1 vs. ExPASy TrEMBL
Match: A0A0A0LIH4 (Gamma-glutamylcyclotransferase family protein OS=Cucumis sativus OX=3659 GN=Csa_2G232990 PE=3 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 3.3e-87
Identity = 161/177 (90.96%), Postives = 168/177 (94.92%), Query Frame = 0


Query: 1   MVAEANQTIPTTLIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLVCGPFRV 60
           MVAEANQTIPTT IFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRT +R PLVCGPF+V
Sbjct: 1   MVAEANQTIPTTFIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTRERFPLVCGPFQV 60

Query: 61  PFLINSPGSGHRVEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDVEATDGGGVVAAEAY 120
           PFLINSPGSGH VEGEVYAVTNRGLGRTDELEGLSRGHY+RLPIDVEA++GGGVVAAEAY
Sbjct: 61  PFLINSPGSGHIVEGEVYAVTNRGLGRTDELEGLSRGHYIRLPIDVEASEGGGVVAAEAY 120

Query: 121 YAHPSYAMELW--KKEGGGVISSYSEKEAKGYVKRKDRPQHISFLDQIRLFLSSSSD 176
           YAHPSYAMELW  KKE GGVISSYSEKEAKGYVKRKDRPQH++FLD I LFLSSSSD
Sbjct: 121 YAHPSYAMELWKKKKEIGGVISSYSEKEAKGYVKRKDRPQHLTFLDHISLFLSSSSD 177

[Close]

BLAST of Lsi10G002860.1 vs. ExPASy TrEMBL
Match: A0A5A7SRA9 (Gamma-glutamylcyclotransferase family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1293G00010 PE=3 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.2e-84
Identity = 156/177 (88.14%), Postives = 165/177 (93.22%), Query Frame = 0


Query: 1   MVAEANQTIPTTLIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLVCGPFRV 60
           MVA+A QT+PTT IFTYGTLKRGFWNHTLLQDLMRTGDASF+GSYRT +R PLVCGPF+V
Sbjct: 1   MVAQAKQTVPTTFIFTYGTLKRGFWNHTLLQDLMRTGDASFVGSYRTRERFPLVCGPFQV 60

Query: 61  PFLINSPGSGHRVEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDVEATDGGGVVAAEAY 120
           PFLINSPGSGH VEGEVYAVT  GLGRTDELEGLSRGHYVRLPIDVEA++GGGVVAAEAY
Sbjct: 61  PFLINSPGSGHIVEGEVYAVTKGGLGRTDELEGLSRGHYVRLPIDVEASEGGGVVAAEAY 120

Query: 121 YAHPSYAMELW--KKEGGGVISSYSEKEAKGYVKRKDRPQHISFLDQIRLFLSSSSD 176
           YAHPSYAMELW  KKE GGVISSYSEKEAKGYVKRKDRPQH++FLD I LFLSSSSD
Sbjct: 121 YAHPSYAMELWKKKKEMGGVISSYSEKEAKGYVKRKDRPQHLTFLDHISLFLSSSSD 177

[Close]

BLAST of Lsi10G002860.1 vs. ExPASy TrEMBL
Match: A0A1S3C9M2 (Gamma-glutamylcyclotransferase family protein OS=Cucumis melo OX=3656 GN=LOC103498361 PE=3 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.2e-84
Identity = 156/177 (88.14%), Postives = 165/177 (93.22%), Query Frame = 0


Query: 1   MVAEANQTIPTTLIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLVCGPFRV 60
           MVA+A QT+PTT IFTYGTLKRGFWNHTLLQDLMRTGDASF+GSYRT +R PLVCGPF+V
Sbjct: 1   MVAQAKQTVPTTFIFTYGTLKRGFWNHTLLQDLMRTGDASFVGSYRTRERFPLVCGPFQV 60

Query: 61  PFLINSPGSGHRVEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDVEATDGGGVVAAEAY 120
           PFLINSPGSGH VEGEVYAVT  GLGRTDELEGLSRGHYVRLPIDVEA++GGGVVAAEAY
Sbjct: 61  PFLINSPGSGHIVEGEVYAVTKGGLGRTDELEGLSRGHYVRLPIDVEASEGGGVVAAEAY 120

Query: 121 YAHPSYAMELW--KKEGGGVISSYSEKEAKGYVKRKDRPQHISFLDQIRLFLSSSSD 176
           YAHPSYAMELW  KKE GGVISSYSEKEAKGYVKRKDRPQH++FLD I LFLSSSSD
Sbjct: 121 YAHPSYAMELWKKKKEMGGVISSYSEKEAKGYVKRKDRPQHLTFLDHISLFLSSSSD 177

[Close]

BLAST of Lsi10G002860.1 vs. ExPASy TrEMBL
Match: A0A6J1FUF8 (Gamma-glutamylcyclotransferase family protein OS=Cucurbita moschata OX=3662 GN=LOC111448896 PE=3 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.0e-80
Identity = 151/180 (83.89%), Postives = 162/180 (90.00%), Query Frame = 0


Query: 1   MVAEANQTIPTTLIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLVCGPFRV 60
           MVA+ANQTIPTTLIFTYGTLKRGFWNHTLLQDLMRTGDASF+GSYRT +  PLVCGP+RV
Sbjct: 1   MVAKANQTIPTTLIFTYGTLKRGFWNHTLLQDLMRTGDASFVGSYRTKESFPLVCGPYRV 60

Query: 61  PFLINSPGSGHRVEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDVEATDG--GGVVAAE 120
           PFLINSPGSG+RVEGEVYAVT RGL RTDELEGL+ GHYVRLPIDV   +G  GGVV AE
Sbjct: 61  PFLINSPGSGYRVEGEVYAVTERGLARTDELEGLNNGHYVRLPIDVVDANGGVGGVVVAE 120

Query: 121 AYYAHPSYAMELW-----KKEGGGVISSYSEKEAKGYVKRKDRPQHISFLDQIRLFLSSS 174
           AYYAHPSYAMELW     KK+GGGVI+SYSEKEAKGYVKRKDRPQH+SFLD IR+FLSSS
Sbjct: 121 AYYAHPSYAMELWRKEGKKKDGGGVINSYSEKEAKGYVKRKDRPQHLSFLDHIRIFLSSS 180

[Close]

BLAST of Lsi10G002860.1 vs. ExPASy TrEMBL
Match: A0A6J1JH21 (Gamma-glutamylcyclotransferase family protein OS=Cucurbita maxima OX=3661 GN=LOC111484306 PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.1e-79
Identity = 149/180 (82.78%), Postives = 161/180 (89.44%), Query Frame = 0


Query: 1   MVAEANQTIPTTLIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLVCGPFRV 60
           MVA+ NQTIPTTLIFTYGTLKRGFWNH LLQDLMRTGDASF+GSYRT +  PLVCGP+RV
Sbjct: 1   MVAKTNQTIPTTLIFTYGTLKRGFWNHMLLQDLMRTGDASFVGSYRTKESFPLVCGPYRV 60

Query: 61  PFLINSPGSGHRVEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDVEATDG--GGVVAAE 120
           PFLINSPGSG+RVEGEVYAVT+RGL RTDELEGL+ GHYVRLPIDV   +G  GGVV AE
Sbjct: 61  PFLINSPGSGYRVEGEVYAVTDRGLARTDELEGLNNGHYVRLPIDVVDANGGVGGVVVAE 120

Query: 121 AYYAHPSYAMELW-----KKEGGGVISSYSEKEAKGYVKRKDRPQHISFLDQIRLFLSSS 174
           AYYAHPSYAMELW     KK+GGGVI+SYSEKEAKGYVKRKDRPQH+SFLD IR+FLSSS
Sbjct: 121 AYYAHPSYAMELWRKEGKKKDGGGVINSYSEKEAKGYVKRKDRPQHLSFLDHIRIFLSSS 180

[Close]

BLAST of Lsi10G002860.1 vs. NCBI nr
Match: XP_038901736.1 (putative gamma-glutamylcyclotransferase At3g02910 [Benincasa hispida])

HSP 1 Score: 334.0 bits (855), Expect = 8.0e-88
Identity = 163/176 (92.61%), Postives = 168/176 (95.45%), Query Frame = 0


Query: 1   MVAEANQTIPTTLIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLVCGPFRV 60
           MVAEANQTIP TLIFTYGTLKRGFWNHTLLQDLMRTGDASFIG YRT +  PLVCGP++V
Sbjct: 1   MVAEANQTIPRTLIFTYGTLKRGFWNHTLLQDLMRTGDASFIGIYRTMEPFPLVCGPYQV 60

Query: 61  PFLINSPGSGHRVEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDVEATDGGGVVAAEAY 120
           PFLINSPGSGHRVEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDVEATDGGGVVAAEAY
Sbjct: 61  PFLINSPGSGHRVEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDVEATDGGGVVAAEAY 120

Query: 121 YAHPSYAMELWKKE--GGGVISSYSEKEAKGYVKRKDRPQHISFLDQIRLFLSSSS 175
           YAHPSYAMELWKK+  GGGVISSYSEKEAKGYVKRKDRPQH+SFLD IRLFLSSSS
Sbjct: 121 YAHPSYAMELWKKKESGGGVISSYSEKEAKGYVKRKDRPQHLSFLDHIRLFLSSSS 176

[Close]

BLAST of Lsi10G002860.1 vs. NCBI nr
Match: XP_004145362.1 (putative gamma-glutamylcyclotransferase At3g02910 [Cucumis sativus])

HSP 1 Score: 330.9 bits (847), Expect = 6.8e-87
Identity = 161/177 (90.96%), Postives = 168/177 (94.92%), Query Frame = 0


Query: 1   MVAEANQTIPTTLIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLVCGPFRV 60
           MVAEANQTIPTT IFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRT +R PLVCGPF+V
Sbjct: 1   MVAEANQTIPTTFIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTRERFPLVCGPFQV 60

Query: 61  PFLINSPGSGHRVEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDVEATDGGGVVAAEAY 120
           PFLINSPGSGH VEGEVYAVTNRGLGRTDELEGLSRGHY+RLPIDVEA++GGGVVAAEAY
Sbjct: 61  PFLINSPGSGHIVEGEVYAVTNRGLGRTDELEGLSRGHYIRLPIDVEASEGGGVVAAEAY 120

Query: 121 YAHPSYAMELW--KKEGGGVISSYSEKEAKGYVKRKDRPQHISFLDQIRLFLSSSSD 176
           YAHPSYAMELW  KKE GGVISSYSEKEAKGYVKRKDRPQH++FLD I LFLSSSSD
Sbjct: 121 YAHPSYAMELWKKKKEIGGVISSYSEKEAKGYVKRKDRPQHLTFLDHISLFLSSSSD 177

[Close]

BLAST of Lsi10G002860.1 vs. NCBI nr
Match: XP_008459163.1 (PREDICTED: putative gamma-glutamylcyclotransferase At3g02910 [Cucumis melo] >KAA0032091.1 putative gamma-glutamylcyclotransferase [Cucumis melo var. makuwa] >TYK20953.1 putative gamma-glutamylcyclotransferase [Cucumis melo var. makuwa])

HSP 1 Score: 322.4 bits (825), Expect = 2.4e-84
Identity = 156/177 (88.14%), Postives = 165/177 (93.22%), Query Frame = 0


Query: 1   MVAEANQTIPTTLIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLVCGPFRV 60
           MVA+A QT+PTT IFTYGTLKRGFWNHTLLQDLMRTGDASF+GSYRT +R PLVCGPF+V
Sbjct: 1   MVAQAKQTVPTTFIFTYGTLKRGFWNHTLLQDLMRTGDASFVGSYRTRERFPLVCGPFQV 60

Query: 61  PFLINSPGSGHRVEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDVEATDGGGVVAAEAY 120
           PFLINSPGSGH VEGEVYAVT  GLGRTDELEGLSRGHYVRLPIDVEA++GGGVVAAEAY
Sbjct: 61  PFLINSPGSGHIVEGEVYAVTKGGLGRTDELEGLSRGHYVRLPIDVEASEGGGVVAAEAY 120

Query: 121 YAHPSYAMELW--KKEGGGVISSYSEKEAKGYVKRKDRPQHISFLDQIRLFLSSSSD 176
           YAHPSYAMELW  KKE GGVISSYSEKEAKGYVKRKDRPQH++FLD I LFLSSSSD
Sbjct: 121 YAHPSYAMELWKKKKEMGGVISSYSEKEAKGYVKRKDRPQHLTFLDHISLFLSSSSD 177

[Close]

BLAST of Lsi10G002860.1 vs. NCBI nr
Match: KAG6571165.1 (putative gamma-glutamylcyclotransferase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010971.1 putative gamma-glutamylcyclotransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 309.7 bits (792), Expect = 1.6e-80
Identity = 151/180 (83.89%), Postives = 163/180 (90.56%), Query Frame = 0


Query: 1   MVAEANQTIPTTLIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLVCGPFRV 60
           MVA+ANQTIPTTLIFTYGTLKRGFWNHTLLQDLMRTGDASF+GSYRT +  PLVCGP+RV
Sbjct: 1   MVAKANQTIPTTLIFTYGTLKRGFWNHTLLQDLMRTGDASFVGSYRTKESFPLVCGPYRV 60

Query: 61  PFLINSPGSGHRVEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDVEATDG--GGVVAAE 120
           PFLINSPGSG+RVEGEVYAVT+RGL RTDELEGL+ GHYVRLPIDV   +G  GGVV AE
Sbjct: 61  PFLINSPGSGYRVEGEVYAVTDRGLARTDELEGLNNGHYVRLPIDVVDANGGVGGVVVAE 120

Query: 121 AYYAHPSYAMELW-----KKEGGGVISSYSEKEAKGYVKRKDRPQHISFLDQIRLFLSSS 174
           AYYAHPSYAMELW     KK+GGGVI+SYSEKEAKGYVKRKDRPQH+SFLD IR+FLSSS
Sbjct: 121 AYYAHPSYAMELWRKEGKKKDGGGVINSYSEKEAKGYVKRKDRPQHLSFLDHIRIFLSSS 180

[Close]

BLAST of Lsi10G002860.1 vs. NCBI nr
Match: XP_022944446.1 (putative gamma-glutamylcyclotransferase At3g02910 [Cucurbita moschata])

HSP 1 Score: 309.3 bits (791), Expect = 2.1e-80
Identity = 151/180 (83.89%), Postives = 162/180 (90.00%), Query Frame = 0


Query: 1   MVAEANQTIPTTLIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLVCGPFRV 60
           MVA+ANQTIPTTLIFTYGTLKRGFWNHTLLQDLMRTGDASF+GSYRT +  PLVCGP+RV
Sbjct: 1   MVAKANQTIPTTLIFTYGTLKRGFWNHTLLQDLMRTGDASFVGSYRTKESFPLVCGPYRV 60

Query: 61  PFLINSPGSGHRVEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDVEATDG--GGVVAAE 120
           PFLINSPGSG+RVEGEVYAVT RGL RTDELEGL+ GHYVRLPIDV   +G  GGVV AE
Sbjct: 61  PFLINSPGSGYRVEGEVYAVTERGLARTDELEGLNNGHYVRLPIDVVDANGGVGGVVVAE 120

Query: 121 AYYAHPSYAMELW-----KKEGGGVISSYSEKEAKGYVKRKDRPQHISFLDQIRLFLSSS 174
           AYYAHPSYAMELW     KK+GGGVI+SYSEKEAKGYVKRKDRPQH+SFLD IR+FLSSS
Sbjct: 121 AYYAHPSYAMELWRKEGKKKDGGGVINSYSEKEAKGYVKRKDRPQHLSFLDHIRIFLSSS 180

[Close]

BLAST of Lsi10G002860.1 vs. TAIR 10
Match: AT3G02910.1 (AIG2-like (avirulence induced gene) family protein )

HSP 1 Score: 221.1 bits (562), Expect = 7.1e-58
Identity = 105/177 (59.32%), Postives = 128/177 (72.32%), Query Frame = 0


Query: 11  TTLIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLVCGPFRVPFLINSPGSG 70
           TTL+FTYGTLKRGF NH L+QDL+R+GDASF G Y+T  + PLVCGP+RVPFL+N PGSG
Sbjct: 11  TTLVFTYGTLKRGFSNHVLMQDLIRSGDASFKGVYQTLDKYPLVCGPYRVPFLLNKPGSG 70

Query: 71  HRVEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDVEA------------TDGGGVVAAE 130
           + V GE+YAV+ RGL R DELEG+SRGHY+R PI + A            T+       E
Sbjct: 71  YHVNGELYAVSPRGLSRLDELEGISRGHYIRQPIRLAAAEEEEEEEGDLETEAPSSCVVE 130

Query: 131 AYYAHPSYAMELWKKEGGGVISSYSEKEAKGYVKRKDRPQHISFLDQIRLFLSSSSD 176
           AYYAH SY  ELW++  G    +Y+E EA+GYVKR DRPQH+SFLD IR+F+SS  D
Sbjct: 131 AYYAHKSYEEELWRRNRGRSFGAYTENEARGYVKRNDRPQHLSFLDHIRIFVSSPCD 187

[Close]

BLAST of Lsi10G002860.1 vs. TAIR 10
Match: AT5G46720.1 (AIG2-like (avirulence induced gene) family protein )

HSP 1 Score: 162.5 bits (410), Expect = 3.0e-40
Identity = 82/168 (48.81%), Postives = 108/168 (64.29%), Query Frame = 0


Query: 13  LIFTYGTLKRGFWNHTLLQDLMRTGDASFIGSYRTAKRLPLVCGPFRVPFLINSPGSGHR 72
           LIF YGTLKR   NH LL+DL+ T DA +IG   T  + PLV G + +P+LIN  GSG +
Sbjct: 8   LIFVYGTLKRNHRNHFLLEDLISTNDAVYIGQRTTRLQYPLVTGLYGIPYLINKSGSGQK 67

Query: 73  VEGEVYAVTNRGLGRTDELEGLSRGHYVRLPIDV-----EATDGGGVVAAEAYYAHPSYA 132
           + GE+Y+V+ RGL R DELEG+   HY RLPI+V     E  +  GVV AEAY+AH  + 
Sbjct: 68  IRGELYSVSKRGLVRLDELEGIKVNHYERLPIEVIEEDDEEEESNGVVLAEAYFAHFGFG 127

Query: 133 MELWKKEGGGVISSYSEKEAKGYVKRKDRPQHISFLDQIRLFLSSSSD 176
             LW+K+G   +  + E +   YV+ KDRP   S LD+I  F+SSS+D
Sbjct: 128 ERLWEKKGKCGMCEFGENDGVLYVRPKDRPMFSSVLDEIETFVSSSND 175

The following BLAST results are available for this feature:

BLAST of Lsi10G002860.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M8T3	9.9e-57	59.32	Putative gamma-glutamylcyclotransferase At3g02910 OS=Arabidopsis thaliana OX=370...	[more]
Q66I06	2.2e-11	40.18	Gamma-glutamylaminecyclotransferase B OS=Danio rerio OX=7955 GN=ggact.2 PE=2 SV=...	[more]
Q9BVM4	2.2e-11	49.38	Gamma-glutamylaminecyclotransferase OS=Homo sapiens OX=9606 GN=GGACT PE=1 SV=2	[more]
Q9W0Y2	6.3e-11	45.00	Putative gamma-glutamylcyclotransferase CG2811 OS=Drosophila melanogaster OX=722...	[more]
Q4KM86	3.1e-10	37.70	Gamma-glutamylaminecyclotransferase OS=Rattus norvegicus OX=10116 GN=Ggact PE=2 ...	[more]

BLAST of Lsi10G002860.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LIH4	3.3e-87	90.96	Gamma-glutamylcyclotransferase family protein OS=Cucumis sativus OX=3659 GN=Csa_...	[more]
A0A5A7SRA9	1.2e-84	88.14	Gamma-glutamylcyclotransferase family protein OS=Cucumis melo var. makuwa OX=119...	[more]
A0A1S3C9M2	1.2e-84	88.14	Gamma-glutamylcyclotransferase family protein OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A6J1FUF8	1.0e-80	83.89	Gamma-glutamylcyclotransferase family protein OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1JH21	1.1e-79	82.78	Gamma-glutamylcyclotransferase family protein OS=Cucurbita maxima OX=3661 GN=LOC...	[more]

BLAST of Lsi10G002860.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901736.1	8.0e-88	92.61	putative gamma-glutamylcyclotransferase At3g02910 [Benincasa hispida]	[more]
XP_004145362.1	6.8e-87	90.96	putative gamma-glutamylcyclotransferase At3g02910 [Cucumis sativus]	[more]
XP_008459163.1	2.4e-84	88.14	PREDICTED: putative gamma-glutamylcyclotransferase At3g02910 [Cucumis melo] >KAA...	[more]
KAG6571165.1	1.6e-80	83.89	putative gamma-glutamylcyclotransferase, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_022944446.1	2.1e-80	83.89	putative gamma-glutamylcyclotransferase At3g02910 [Cucurbita moschata]	[more]

BLAST of Lsi10G002860.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G02910.1	7.1e-58	59.32	AIG2-like (avirulence induced gene) family protein	[more]
AT5G46720.1	3.0e-40	48.81	AIG2-like (avirulence induced gene) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.490.10		coord: 3..156 e-value: 6.1E-36 score: 125.5
None	No IPR available	PANTHER	PTHR12510:SF16	BNACNNG06060D PROTEIN	coord: 9..175
IPR009288	Gamma-glutamylcyclotransferase, AIG2-like domain	PFAM	PF06094	GGACT	coord: 14..124 e-value: 9.3E-20 score: 71.5
IPR039126	Gamma-glutamylaminecyclotransferase	PANTHER	PTHR12510	TROPONIN C-AKIN-1 PROTEIN	coord: 9..175
IPR013024	Gamma-glutamyl cyclotransferase-like	CDD	cd06661	GGCT_like	coord: 14..120 e-value: 5.81353E-16 score: 67.755
IPR036568	Gamma-glutamyl cyclotransferase-like superfamily	SUPERFAMILY	110857	Gamma-glutamyl cyclotransferase-like	coord: 11..130

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi10G002860	Lsi10G002860	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi10G002860.1.exon.1	Lsi10G002860.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi10G002860.1.CDS.1	Lsi10G002860.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi10G002860.1	Lsi10G002860.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005829	cytosol
molecular_function	GO:0061929	gamma-glutamylaminecyclotransferase activity
molecular_function	GO:0016740	transferase activity