Lsi09G015930.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G015930.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	sphingoid long-chain bases kinase 2, mitochondrial
Location	chr09: 24050305 .. 24051348 (-)
Sequence length	681
RNA-Seq Expression	Lsi09G015930.1
Synteny	Lsi09G015930.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCTGTTTGTTAACACAAACATCACATTAGCATTGGTAGTTCATGTAAGGTATTCTGCAGGGTTGAGGTCCCGAATTGATGTTGGAGTTGTCAATGAAGAAAATGGAGGGTATCATTACTTTGCAAATGTTGCAGACATTCATTTGTAAGGTTAATCTAAACGGTATCATTGATGCTAACCGAAAAACTATGGTAATTCAGGACATCTAATTATTATATTGCATGGATCTTTTTGCAGGAGTGCAAAGGCTGGTTTTTATGCTGCAAGATACAAGAAATTTGGAAACTTATGCTATGTGATTGGTGCTTTGCAAGGCTTTATGGGACACCAAAATAAAGATTTCAAGATTAAGGTCAGCATATAAAAGAAACTCTAGGTATTTTTTTTTTTATTTGTTTTATGCAATAATTGTGGTGCAGTGCCTTAATGTGGTTCCAAAATGTAGACCATTTTGGAGGGGGGAGAGAGAGAGAGAGACAGTATTTTTACCATCTCGATTTGATTTCATCTCTGTAGAATGCTATAACTTACAGGTTGATGACGGTGAATGGGAGCTGTATCCACAAGTAACTGCCCTATGCATTGGAAATGCTAAATACTTCGGTGGTGGTATGAAAATCGTGCCGAATGCCGATCCTTCCAATGGGAATCTAGAGGTAGAATTCATTTCCGTGGAATTTTATCTGATTGCACTATTTCCTTTATTGTCTCCTTATGTGATACTTGCCCTCTGCTTTCTTTGCAGGTAGTAATTCTTCAGGATTTCAAGTGGTACGACTTTATTCTGAATTTACATAAAATCTACAATGGGACACATTTGACAGTGAAAAATGTAACATCAAGAAGGTTTGTCATCTCTTTAAGAATTACAATGTGTCTAGACTTTCCATTCTGCGTTTCAATTTTCTCTCTCCAACATCTGTTTTAGGCTGAGATTTGTGCAAGTAAAGGTGAAGAAACTATAACTGAACTAGAATTGCATGAAGAATTTGTAGTGTTAATTTCTGTCAAGTTTTTGCTTGAGAAATCTTACCAATAA

mRNA sequence

ATGGCTCTGTTTGTTAACACAAACATCACATTAGCATTGGTAGTTCATGTAAGGTATTCTGCAGGGTTGAGGTCCCGAATTGATGTTGGAGTTGTCAATGAAGAAAATGGAGGGTATCATTACTTTGCAAATGTTGCAGACATTCATTTGAGTGCAAAGGCTGGTTTTTATGCTGCAAGATACAAGAAATTTGGAAACTTATGCTATGTGATTGGTGCTTTGCAAGGCTTTATGGGACACCAAAATAAAGATTTCAAGATTAAGGTTGATGACGGTGAATGGGAGCTGTATCCACAAGTAACTGCCCTATGCATTGGAAATGCTAAATACTTCGGTGGTGGTATGAAAATCGTGCCGAATGCCGATCCTTCCAATGGGAATCTAGAGGTAGTAATTCTTCAGGATTTCAAGTGGTACGACTTTATTCTGAATTTACATAAAATCTACAATGGGACACATTTGACAGTGAAAAATGTAACATCAAGAAGGTTTGTCATCTCTTTAAGAATTACAATGTGTCTAGACTTTCCATTCTGCGTTTCAATTTTCTCTCTCCAACATCTGTTTTAGGCTGAGATTTGTGCAAGTAAAGGTGAAGAAACTATAACTGAACTAGAATTGCATGAAGAATTTGTAGTGTTAATTTCTGTCAAGTTTTTGCTTGAGAAATCTTACCAATAA

Coding sequence (CDS)

ATGGCTCTGTTTGTTAACACAAACATCACATTAGCATTGGTAGTTCATGTAAGGTATTCTGCAGGGTTGAGGTCCCGAATTGATGTTGGAGTTGTCAATGAAGAAAATGGAGGGTATCATTACTTTGCAAATGTTGCAGACATTCATTTGAGTGCAAAGGCTGGTTTTTATGCTGCAAGATACAAGAAATTTGGAAACTTATGCTATGTGATTGGTGCTTTGCAAGGCTTTATGGGACACCAAAATAAAGATTTCAAGATTAAGGTTGATGACGGTGAATGGGAGCTGTATCCACAAGTAACTGCCCTATGCATTGGAAATGCTAAATACTTCGGTGGTGGTATGAAAATCGTGCCGAATGCCGATCCTTCCAATGGGAATCTAGAGGTAGTAATTCTTCAGGATTTCAAGTGGTACGACTTTATTCTGAATTTACATAAAATCTACAATGGGACACATTTGACAGTGAAAAATGTAACATCAAGAAGGTTTGTCATCTCTTTAAGAATTACAATGTGTCTAGACTTTCCATTCTGCGTTTCAATTTTCTCTCTCCAACATCTGTTTTAG

Protein sequence

MALFVNTNITLALVVHVRYSAGLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGFMGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFKWYDFILNLHKIYNGTHLTVKNVTSRRFVISLRITMCLDFPFCVSIFSLQHLF

Homology

[Close]

BLAST of Lsi09G015930.1 vs. ExPASy Swiss-Prot
Match: O82359 (Sphingoid long-chain bases kinase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=LCKB2 PE=2 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 9.7e-66
Identity = 110/145 (75.86%), Postives = 129/145 (88.97%), Query Frame = 0


Query: 18  RYSAGLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF 77
           R + G+RSRIDVGV+++E    HYF NVAD+HLSAKAGFYA++YKKFGNLCYVIGALQ F
Sbjct: 177 RIARGMRSRIDVGVIDKEGKDLHYFINVADVHLSAKAGFYASKYKKFGNLCYVIGALQAF 236

Query: 78  MGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           MGH N+D +I+V+ GEWE+YPQVTALC+GNAKYFGGGMKI PNA P NGNLEVV+LQ+FK
Sbjct: 237 MGHHNQDMRIRVNGGEWEIYPQVTALCVGNAKYFGGGMKITPNAIPGNGNLEVVVLQNFK 296

Query: 138 WYDFILNLHKIYNGTHLTVKNVTSR 163
           WYDF+L LHK+YNGTHL+V NVTSR
Sbjct: 297 WYDFVLKLHKLYNGTHLSVNNVTSR 321

[Close]

BLAST of Lsi09G015930.1 vs. ExPASy Swiss-Prot
Match: P9WP28 (Diacylglycerol kinase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=dagK PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-08
Identity = 40/143 (27.97%), Postives = 66/143 (46.15%), Query Frame = 0


Query: 22  GLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYAARYK-KFGNLCYVIGALQGFMGH 81
           G    ID+G + ++NG   +F  VA     +     A R +   G + Y I  L      
Sbjct: 126 GWTETIDLGRIQDDNGIEKWFGTVAATGFDSLVNDRANRMRWPHGRMRYYIAMLAELSRL 185

Query: 82  QNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFKWYD 141
           +   F++ V DG  E+   +T    GN + +GGG+ I PNAD S+G L++ + Q      
Sbjct: 186 RPLPFRL-VLDGTEEIVADLTLADFGNTRSYGGGLLICPNADHSDGLLDITMAQSDSRTK 245

Query: 142 FILNLHKIYNGTHLTVKNVTSRR 164
            +     I+ G H+ +  V++ R
Sbjct: 246 LLRLFPTIFKGAHVELDEVSTTR 267

[Close]

BLAST of Lsi09G015930.1 vs. ExPASy Swiss-Prot
Match: P9WP29 (Diacylglycerol kinase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=dagK PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-08
Identity = 40/143 (27.97%), Postives = 66/143 (46.15%), Query Frame = 0


Query: 22  GLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYAARYK-KFGNLCYVIGALQGFMGH 81
           G    ID+G + ++NG   +F  VA     +     A R +   G + Y I  L      
Sbjct: 126 GWTETIDLGRIQDDNGIEKWFGTVAATGFDSLVNDRANRMRWPHGRMRYYIAMLAELSRL 185

Query: 82  QNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFKWYD 141
           +   F++ V DG  E+   +T    GN + +GGG+ I PNAD S+G L++ + Q      
Sbjct: 186 RPLPFRL-VLDGTEEIVADLTLADFGNTRSYGGGLLICPNADHSDGLLDITMAQSDSRTK 245

Query: 142 FILNLHKIYNGTHLTVKNVTSRR 164
            +     I+ G H+ +  V++ R
Sbjct: 246 LLRLFPTIFKGAHVELDEVSTTR 267

[Close]

BLAST of Lsi09G015930.1 vs. ExPASy TrEMBL
Match: A0A0A0KD28 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G006770 PE=4 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 2.2e-81
Identity = 148/162 (91.36%), Postives = 154/162 (95.06%), Query Frame = 0


Query: 1   MALFVNTNITLALVVHVRYSAGLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYAAR 60
           MALFV+TNI L LVVH RY AGLR+RIDVGVVNE+NG  HYFANVADIHLSAKAGFYAAR
Sbjct: 1   MALFVHTNIALPLVVHARYCAGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAGFYAAR 60

Query: 61  YKKFGNLCYVIGALQGFMGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPN 120
           YKKFGNLCYVIGALQGFMGHQN+DF+IKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPN
Sbjct: 61  YKKFGNLCYVIGALQGFMGHQNRDFRIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPN 120

Query: 121 ADPSNGNLEVVILQDFKWYDFILNLHKIYNGTHLTVKNVTSR 163
            DPSN +LEVVILQDFKWYDFILNLHKIYNGTHLTVKNVTSR
Sbjct: 121 VDPSNRSLEVVILQDFKWYDFILNLHKIYNGTHLTVKNVTSR 162

[Close]

BLAST of Lsi09G015930.1 vs. ExPASy TrEMBL
Match: A0A5D3BVA2 (Sphingoid long-chain bases kinase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001350 PE=4 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 5.3e-75
Identity = 135/145 (93.10%), Postives = 141/145 (97.24%), Query Frame = 0


Query: 18  RYSAGLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF 77
           R + GLR+RIDVGVVNE+NG  HYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF
Sbjct: 179 RIAKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF 238

Query: 78  MGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           MGHQN+DFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNG+LEVVILQDFK
Sbjct: 239 MGHQNRDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFK 298

Query: 138 WYDFILNLHKIYNGTHLTVKNVTSR 163
           WYDFILNLHKIYNGTHLTVKNVTS+
Sbjct: 299 WYDFILNLHKIYNGTHLTVKNVTSK 323

[Close]

BLAST of Lsi09G015930.1 vs. ExPASy TrEMBL
Match: A0A1S3C7U6 (sphingoid long-chain bases kinase 2, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103497689 PE=4 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 5.3e-75
Identity = 135/145 (93.10%), Postives = 141/145 (97.24%), Query Frame = 0


Query: 18  RYSAGLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF 77
           R + GLR+RIDVGVVNE+NG  HYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF
Sbjct: 179 RIAKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF 238

Query: 78  MGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           MGHQN+DFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNG+LEVVILQDFK
Sbjct: 239 MGHQNRDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFK 298

Query: 138 WYDFILNLHKIYNGTHLTVKNVTSR 163
           WYDFILNLHKIYNGTHLTVKNVTS+
Sbjct: 299 WYDFILNLHKIYNGTHLTVKNVTSK 323

[Close]

BLAST of Lsi09G015930.1 vs. ExPASy TrEMBL
Match: A0A6J1HNB5 (sphingoid long-chain bases kinase 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111464490 PE=4 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 9.1e-75
Identity = 133/145 (91.72%), Postives = 142/145 (97.93%), Query Frame = 0


Query: 18  RYSAGLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF 77
           R + GLRSRID+GVVN+ENG YHYFANVADIHLSAKAGFYAARYK+FGNLCYVIGALQGF
Sbjct: 177 RIAKGLRSRIDIGVVNKENGEYHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 236

Query: 78  MGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           +GH+NKDFKIKVD+GEWELYPQVT+LCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK
Sbjct: 237 IGHKNKDFKIKVDNGEWELYPQVTSLCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 296

Query: 138 WYDFILNLHKIYNGTHLTVKNVTSR 163
           WYDFILNLHKIYNGTHLTVKNV+SR
Sbjct: 297 WYDFILNLHKIYNGTHLTVKNVSSR 321

[Close]

BLAST of Lsi09G015930.1 vs. ExPASy TrEMBL
Match: A0A6J1KFE4 (sphingoid long-chain bases kinase 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111494546 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 1.0e-73
Identity = 131/145 (90.34%), Postives = 141/145 (97.24%), Query Frame = 0


Query: 18  RYSAGLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF 77
           R + GLRSRID+GVVN+ENG YHYFANVADIHLSAKAGFYAARYK+FGNLCYVIGALQGF
Sbjct: 177 RIAKGLRSRIDIGVVNKENGEYHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 236

Query: 78  MGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           +GH+NKD KIKVD+GEWELYPQVT+LCIGNAKYFGGGMKIVPNADPSNGNLE+VILQDFK
Sbjct: 237 IGHKNKDLKIKVDNGEWELYPQVTSLCIGNAKYFGGGMKIVPNADPSNGNLELVILQDFK 296

Query: 138 WYDFILNLHKIYNGTHLTVKNVTSR 163
           WYDFILNLHKIYNGTHLTVKNV+SR
Sbjct: 297 WYDFILNLHKIYNGTHLTVKNVSSR 321

[Close]

BLAST of Lsi09G015930.1 vs. NCBI nr
Match: XP_038876180.1 (sphingoid long-chain bases kinase 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 299.3 bits (765), Expect = 2.4e-77
Identity = 139/145 (95.86%), Postives = 142/145 (97.93%), Query Frame = 0


Query: 18  RYSAGLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF 77
           R + GLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYA+RYKKFGNLCYVIGALQGF
Sbjct: 178 RIAKGLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYASRYKKFGNLCYVIGALQGF 237

Query: 78  MGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           MGHQN+DFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADP NGNLEVVILQDFK
Sbjct: 238 MGHQNRDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPCNGNLEVVILQDFK 297

Query: 138 WYDFILNLHKIYNGTHLTVKNVTSR 163
           WYDFILNLHKIYNGTHLTVKNVTSR
Sbjct: 298 WYDFILNLHKIYNGTHLTVKNVTSR 322

[Close]

BLAST of Lsi09G015930.1 vs. NCBI nr
Match: XP_008458171.1 (PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial [Cucumis melo] >TYK03040.1 sphingoid long-chain bases kinase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 290.4 bits (742), Expect = 1.1e-74
Identity = 135/145 (93.10%), Postives = 141/145 (97.24%), Query Frame = 0


Query: 18  RYSAGLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF 77
           R + GLR+RIDVGVVNE+NG  HYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF
Sbjct: 179 RIAKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF 238

Query: 78  MGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           MGHQN+DFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNG+LEVVILQDFK
Sbjct: 239 MGHQNRDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFK 298

Query: 138 WYDFILNLHKIYNGTHLTVKNVTSR 163
           WYDFILNLHKIYNGTHLTVKNVTS+
Sbjct: 299 WYDFILNLHKIYNGTHLTVKNVTSK 323

[Close]

BLAST of Lsi09G015930.1 vs. NCBI nr
Match: XP_031743330.1 (sphingoid long-chain bases kinase 2, mitochondrial [Cucumis sativus] >KAE8646552.1 hypothetical protein Csa_005226 [Cucumis sativus])

HSP 1 Score: 290.4 bits (742), Expect = 1.1e-74
Identity = 135/145 (93.10%), Postives = 141/145 (97.24%), Query Frame = 0


Query: 18  RYSAGLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF 77
           R + GLR+RIDVGVVNE+NG  HYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF
Sbjct: 179 RIAKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF 238

Query: 78  MGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           MGHQN+DF+IKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNG+LEVVILQDFK
Sbjct: 239 MGHQNRDFRIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFK 298

Query: 138 WYDFILNLHKIYNGTHLTVKNVTSR 163
           WYDFILNLHKIYNGTHLTVKNVTSR
Sbjct: 299 WYDFILNLHKIYNGTHLTVKNVTSR 323

[Close]

BLAST of Lsi09G015930.1 vs. NCBI nr
Match: KAG7025983.1 (Sphingoid long-chain bases kinase 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 289.7 bits (740), Expect = 1.9e-74
Identity = 133/145 (91.72%), Postives = 142/145 (97.93%), Query Frame = 0


Query: 18  RYSAGLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF 77
           R + GLRSRID+GVVN+ENG YHYFANVADIHLSAKAGFYAARYK+FGNLCYVIGALQGF
Sbjct: 177 RIAKGLRSRIDIGVVNKENGEYHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 236

Query: 78  MGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           +GH+NKDFKIKVD+GEWELYPQVT+LCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK
Sbjct: 237 IGHKNKDFKIKVDNGEWELYPQVTSLCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 296

Query: 138 WYDFILNLHKIYNGTHLTVKNVTSR 163
           WYDFILNLHKIYNGTHLTVKNV+SR
Sbjct: 297 WYDFILNLHKIYNGTHLTVKNVSSR 321

[Close]

BLAST of Lsi09G015930.1 vs. NCBI nr
Match: XP_023514489.1 (sphingoid long-chain bases kinase 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 289.7 bits (740), Expect = 1.9e-74
Identity = 133/145 (91.72%), Postives = 142/145 (97.93%), Query Frame = 0


Query: 18  RYSAGLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF 77
           R + GLRSRID+GVVN+ENG YHYFANVADIHLSAKAGFYAARYK+FGNLCYVIGALQGF
Sbjct: 177 RIAKGLRSRIDIGVVNKENGEYHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 236

Query: 78  MGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           +GH+NKDFKIKVD+GEWELYPQVT+LCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK
Sbjct: 237 IGHKNKDFKIKVDNGEWELYPQVTSLCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 296

Query: 138 WYDFILNLHKIYNGTHLTVKNVTSR 163
           WYDFILNLHKIYNGTHLTVKNV+SR
Sbjct: 297 WYDFILNLHKIYNGTHLTVKNVSSR 321

[Close]

BLAST of Lsi09G015930.1 vs. TAIR 10
Match: AT2G46090.1 (Diacylglycerol kinase family protein )

HSP 1 Score: 251.1 bits (640), Expect = 6.9e-67
Identity = 110/145 (75.86%), Postives = 129/145 (88.97%), Query Frame = 0


Query: 18  RYSAGLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF 77
           R + G+RSRIDVGV+++E    HYF NVAD+HLSAKAGFYA++YKKFGNLCYVIGALQ F
Sbjct: 177 RIARGMRSRIDVGVIDKEGKDLHYFINVADVHLSAKAGFYASKYKKFGNLCYVIGALQAF 236

Query: 78  MGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           MGH N+D +I+V+ GEWE+YPQVTALC+GNAKYFGGGMKI PNA P NGNLEVV+LQ+FK
Sbjct: 237 MGHHNQDMRIRVNGGEWEIYPQVTALCVGNAKYFGGGMKITPNAIPGNGNLEVVVLQNFK 296

Query: 138 WYDFILNLHKIYNGTHLTVKNVTSR 163
           WYDF+L LHK+YNGTHL+V NVTSR
Sbjct: 297 WYDFVLKLHKLYNGTHLSVNNVTSR 321

The following BLAST results are available for this feature:

BLAST of Lsi09G015930.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82359	9.7e-66	75.86	Sphingoid long-chain bases kinase 2, mitochondrial OS=Arabidopsis thaliana OX=37...	[more]
P9WP28	1.1e-08	27.97	Diacylglycerol kinase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) ...	[more]
P9WP29	1.1e-08	27.97	Diacylglycerol kinase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) ...	[more]

BLAST of Lsi09G015930.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KD28	2.2e-81	91.36	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G006770 PE=4 SV=1	[more]
A0A5D3BVA2	5.3e-75	93.10	Sphingoid long-chain bases kinase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A1S3C7U6	5.3e-75	93.10	sphingoid long-chain bases kinase 2, mitochondrial OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1HNB5	9.1e-75	91.72	sphingoid long-chain bases kinase 2, mitochondrial OS=Cucurbita moschata OX=3662...	[more]
A0A6J1KFE4	1.0e-73	90.34	sphingoid long-chain bases kinase 2, mitochondrial OS=Cucurbita maxima OX=3661 G...	[more]

BLAST of Lsi09G015930.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038876180.1	2.4e-77	95.86	sphingoid long-chain bases kinase 2, mitochondrial [Benincasa hispida]	[more]
XP_008458171.1	1.1e-74	93.10	PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial [Cucumis melo] >TY...	[more]
XP_031743330.1	1.1e-74	93.10	sphingoid long-chain bases kinase 2, mitochondrial [Cucumis sativus] >KAE8646552...	[more]
KAG7025983.1	1.9e-74	91.72	Sphingoid long-chain bases kinase 2, mitochondrial [Cucurbita argyrosperma subsp...	[more]
XP_023514489.1	1.9e-74	91.72	sphingoid long-chain bases kinase 2, mitochondrial [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi09G015930.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G46090.1	6.9e-67	75.86	Diacylglycerol kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.60.200.40		coord: 28..180 e-value: 4.2E-25 score: 90.4
None	No IPR available	PANTHER	PTHR12358	SPHINGOSINE KINASE	coord: 18..170
None	No IPR available	PANTHER	PTHR12358:SF94	BNAA04G26670D PROTEIN	coord: 18..170
IPR016064	NAD kinase/diacylglycerol kinase-like domain superfamily	SUPERFAMILY	111331	NAD kinase/diacylglycerol kinase-like	coord: 18..163

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi09G015930	Lsi09G015930	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi09G015930.1.exon.4	Lsi09G015930.1.exon.4	exon
Lsi09G015930.1.exon.3	Lsi09G015930.1.exon.3	exon
Lsi09G015930.1.exon.2	Lsi09G015930.1.exon.2	exon
Lsi09G015930.1.exon.1	Lsi09G015930.1.exon.1	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi09G015930.1.three_prime_UTR.1	Lsi09G015930.1.three_prime_UTR.1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi09G015930.1.CDS.4	Lsi09G015930.1.CDS.4	CDS
Lsi09G015930.1.CDS.3	Lsi09G015930.1.CDS.3	CDS
Lsi09G015930.1.CDS.2	Lsi09G015930.1.CDS.2	CDS
Lsi09G015930.1.CDS.1	Lsi09G015930.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi09G015930.1	Lsi09G015930.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
biological_process	GO:0006671	phytosphingosine metabolic process
biological_process	GO:0045039	protein insertion into mitochondrial inner membrane
biological_process	GO:0009409	response to cold
cellular_component	GO:0042721	TIM22 mitochondrial import inner membrane insertion complex
molecular_function	GO:0003951	NAD+ kinase activity