Lsi08G007420.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G007420.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionProtein kinase 2B
Locationchr08: 15760757 .. 15763520 (-)
Sequence length1074
RNA-Seq ExpressionLsi08G007420.1
SyntenyLsi08G007420.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGACTTGCATCAAGGCAGCAACAAAGACTCACTCAATAATACCCTTCATATTCTTCCTTCTCCCTCAAATGGGAACCCCACCCTTTCTCTTTTACTTTTTCCCCCATTTCTCTCTTCCCTTCCATTTTCATTTCCTAATTCCTCTTCCTTTGCTGCTCATCACTTCCATCCTCTTCCAATGGGTAACTGCTTAGATTCTTCTGCTAAAGTTCAAAATGCCCACTCCTCCCAAGGGACTTCCGGTAACACTCTTTTCTCCTTTTTTTTCCTCTTATTATGTATGTTTTTGCTCTCCTTTGCCCTTATGGTTTCTGGGTTTTCCTTAGATTTTTCATTGCTTCCATCTTGCGCTTGGTTTACTTACTAGGAACTTTTGGTGGATTCTGCTGTTTCTTCTTCTTCTTCTTCTTGTTTCTGGTTTTAGCAAATTTGAGATGAGGTTGCTGGTTTGATGATATTGGTTGAAGTTTCAAATTCTTTATTCTGTTAGGCAAGAAAAAGAGATTTTGAGTCAAATTTTATTTATTAAAGTCAATGTAATTAATATTTTTTATTTGGAGCTTTATGGGAAAAAGGAAGAAAGTAATGGGTTTACTTATATAACCTTTCTAACCTTGATTAATTTAATTGAAAATTTAAGCATGATAAAGTTGAATTAGAAATTTGTTTCAAGTGTCATAAGAAATCAACCATGGCCAAAGCGAGTATAACTCAAACATGTGTTTAATTTCCTCCGCTCCTACCTAGCTATTGTTCTAAAAAAAATCCAACCAATTTTTTTTTATTTTTTTTATCACATATCCTTAAAAAGTAAAAACAAGAATTGAAATTCTTATTCAAGAGGTAACAAACAAGTAAATTCATTTGAAATCTTTCAAAATTTTGCTGGAAACAAACTTGCATGATAACCATTTAGATCCAATTTAATAACCATTTGGGTTTTAAAAATTATGTTTGTTTTATCCTACTTTTTATTCATAGTTTTCAAATTTCCTACGAAATATTTGATTTCCTAGCTCATTTCTAAAAACAAAAACATGTTTTTAAAAACTTTTTTTTTTCTTTTAGTTTTCATAAATTGACTTTGATTTTGAAAATACTTCTAACATGTAGATAACGAATCATAGAAACTAATAGATGGAAGTAGTGTTCATAAGTTTATTTCGAAAATAAAAAACTAAAAATCAAATAGTAACCAAACGAGCCTTGTTTTTAGTTTTTTGGTTTTTTAAAGTTAAGCTAATAAGCTGATACATATTAGATATATGTATTTAAAAGTTTAGAGCTTTATTAATATTTTTAAGTACAGTGTCCAAAAAGTTGCTAAGTTTAAAAAATTTAGAGATATTTCATAAACTTTTAAAACTTCAAACTAAATTGATACAACTTTAGTTTCAAGTGCCGAAGTTGTAATTTAATCTTCAAATCTCATTCACAGGGCGTTCTAATATTAATGCGTTCAAATATGATTTAAGTAATGAATCCCTGACACTGTCTTGTTTTCCTTTATGGATCAGAAATCTCCCAAAGTTCAAGCAAGAAATGCCATCCATTAGTTCCTTCCAATCTTCCTAACTCATTGGCGGATAGGAATAACAATGTATTGAGTCTTCCCACACCAAAAACAGAAGGTGAAATATTGTCATCTTCGAATTTGAAGCCTTTCACTTTCAATGAGCTCAAGAATGCTACTAGAAATTTTCGACCTGACAGCCTGCTAGGTGAAGGGGGATTTGGTTATGTTTTCAAAGGATGGATTGATGAAAACACATTGGCTGCTGCGAGGCCGGGATTGGGAATGGTTGTTGCAGTCAAGAAGCTTAAGCCTGAAGCTTCCCAGGGCCACAAAGAGTGGTTGGTGCGTAGCCTAGTTGATATTATTTTCTTTTTCACTTTTTCTGTTATATGCTTGTGCCTGCAACTAACTGCATATAAAATTCAGACAGAAGTTAATTATTTGGGCAAATTTCATCATCCAAATCTAGTTAAGCTGATTGGGTATTGCTTGGAAGGCGAGAATCGGTTATTGGTATACGAGTTTATGCCGAGGGGGAGCTTGGAGAATCATCTCTTCAGAAGTAAGTTCTGTATGCATTAGATGTACAAAGTGGAAAAAGGGCCTTTACCTTGCTTCTGTTCTATGGATGCTCCATATAGTTGACTCTGCTTGATCTTGTAAAATGCCATTGACTCTGTTTCTCAAACATTCTTCTATGAAATATGTTTGCCTCTCTCTCTCAAATGCTATAATTATCAGTTGTCACATTGTAAAGATAGTTAAGTTCATGTATGTTCACTGATATGAACTGGATGGTCACCTTTGCATCTCGAAATCTGGTTACATGTGACCCTCTTTATAACCTTGATATAATTCAACTAGCTCACATGCTAAGTTCTGTTCTGTTGTGCAGGGGGTTCCCAACCTCTTTCTTGGGCATTGAGGATCAAAGTGGCCATAGCTGCTGCCAGAGGGCTTTCCTTTCTTCATGAAGCCGAATCACCCGTCATATACCGCGATTTCAAGGCATCAAACATCCTACTCGATGCGGTAAGAAACCGATTCTTGTAGAGGAAAACACCCACATCCGTTCTGCATAAATTCTATACAATAAGTGTTGTTGGTAACGCAGGAGTTTAATGCAAAGCTTTCTGATTTTGGTTTGGCGAAGGCTGGTCCCACTGGAGATAGAACTCATGTAACTACTCAAGTTATGGGGACTCGTGGCTATGCTGCGCCTGAATATATTGCTACAGGTTCGTAA

mRNA sequence

ATTGACTTGCATCAAGGCAGCAACAAAGACTCACTCAATAATACCCTTCATATTCTTCCTTCTCCCTCAAATGGGAACCCCACCCTTTCTCTTTTACTTTTTCCCCCATTTCTCTCTTCCCTTCCATTTTCATTTCCTAATTCCTCTTCCTTTGCTGCTCATCACTTCCATCCTCTTCCAATGGGTAACTGCTTAGATTCTTCTGCTAAAGTTCAAAATGCCCACTCCTCCCAAGGGACTTCCGAAATCTCCCAAAGTTCAAGCAAGAAATGCCATCCATTAGTTCCTTCCAATCTTCCTAACTCATTGGCGGATAGGAATAACAATGTATTGAGTCTTCCCACACCAAAAACAGAAGGTGAAATATTGTCATCTTCGAATTTGAAGCCTTTCACTTTCAATGAGCTCAAGAATGCTACTAGAAATTTTCGACCTGACAGCCTGCTAGGTGAAGGGGGATTTGGTTATGTTTTCAAAGGATGGATTGATGAAAACACATTGGCTGCTGCGAGGCCGGGATTGGGAATGGTTGTTGCAGTCAAGAAGCTTAAGCCTGAAGCTTCCCAGGGCCACAAAGAGTGGTTGGTGCGTAGCCTAGTTGATATTATTTTCTTTTTCACTTTTTCTGTTATATGCTTGTGCCTGCAACTAACTGCATATAAAATTCAGACAGAAGTTAATTATTTGGGCAAATTTCATCATCCAAATCTAGTTAAGCTGATTGGGTATTGCTTGGAAGGCGAGAATCGGTTATTGGTATACGAGTTTATGCCGAGGGGGAGCTTGGAGAATCATCTCTTCAGAAGGGGTTCCCAACCTCTTTCTTGGGCATTGAGGATCAAAGTGGCCATAGCTGCTGCCAGAGGGCTTTCCTTTCTTCATGAAGCCGAATCACCCGTCATATACCGCGATTTCAAGGCATCAAACATCCTACTCGATGCGGAGTTTAATGCAAAGCTTTCTGATTTTGGTTTGGCGAAGGCTGGTCCCACTGGAGATAGAACTCATGTAACTACTCAAGTTATGGGGACTCGTGGCTATGCTGCGCCTGAATATATTGCTACAGGTTCGTAA

Coding sequence (CDS)

ATGGGTAACTGCTTAGATTCTTCTGCTAAAGTTCAAAATGCCCACTCCTCCCAAGGGACTTCCGAAATCTCCCAAAGTTCAAGCAAGAAATGCCATCCATTAGTTCCTTCCAATCTTCCTAACTCATTGGCGGATAGGAATAACAATGTATTGAGTCTTCCCACACCAAAAACAGAAGGTGAAATATTGTCATCTTCGAATTTGAAGCCTTTCACTTTCAATGAGCTCAAGAATGCTACTAGAAATTTTCGACCTGACAGCCTGCTAGGTGAAGGGGGATTTGGTTATGTTTTCAAAGGATGGATTGATGAAAACACATTGGCTGCTGCGAGGCCGGGATTGGGAATGGTTGTTGCAGTCAAGAAGCTTAAGCCTGAAGCTTCCCAGGGCCACAAAGAGTGGTTGGTGCGTAGCCTAGTTGATATTATTTTCTTTTTCACTTTTTCTGTTATATGCTTGTGCCTGCAACTAACTGCATATAAAATTCAGACAGAAGTTAATTATTTGGGCAAATTTCATCATCCAAATCTAGTTAAGCTGATTGGGTATTGCTTGGAAGGCGAGAATCGGTTATTGGTATACGAGTTTATGCCGAGGGGGAGCTTGGAGAATCATCTCTTCAGAAGGGGTTCCCAACCTCTTTCTTGGGCATTGAGGATCAAAGTGGCCATAGCTGCTGCCAGAGGGCTTTCCTTTCTTCATGAAGCCGAATCACCCGTCATATACCGCGATTTCAAGGCATCAAACATCCTACTCGATGCGGAGTTTAATGCAAAGCTTTCTGATTTTGGTTTGGCGAAGGCTGGTCCCACTGGAGATAGAACTCATGTAACTACTCAAGTTATGGGGACTCGTGGCTATGCTGCGCCTGAATATATTGCTACAGGTTCGTAA

Protein sequence

MGNCLDSSAKVQNAHSSQGTSEISQSSSKKCHPLVPSNLPNSLADRNNNVLSLPTPKTEGEILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMVVAVKKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNLVKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATGS
Homology
BLAST of Lsi08G007420.1 vs. ExPASy Swiss-Prot
Match: O49840 (Probable serine/threonine-protein kinase PBL3 OS=Arabidopsis thaliana OX=3702 GN=PBL3 PE=1 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 4.2e-108
Identity = 204/299 (68.23%), Postives = 232/299 (77.59%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSSQGTSEISQS---SSKKCHPLVPSNLPNSLADRNNNVLSLPTPK 60
           MGNCLDSSAKV ++  S   +  S S   SSK     VPS+L     +  ++V SLPTP+
Sbjct: 1   MGNCLDSSAKVDSSSHSPHANSASLSSRVSSKTSRSTVPSSLS---INSYSSVESLPTPR 60

Query: 61  TEGEILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMV 120
           TEGEILSS NLK FTFNELKNATRNFRPDSLLGEGGFGYVFKGWID  TL A++PG G+V
Sbjct: 61  TEGEILSSPNLKAFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDGTTLTASKPGSGIV 120

Query: 121 VAVKKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNL 180
           VAVKKLK E  QGHKEWL                            TEVNYLG+  HPNL
Sbjct: 121 VAVKKLKTEGYQGHKEWL----------------------------TEVNYLGQLSHPNL 180

Query: 181 VKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAE 240
           VKL+GYC+EGENRLLVYEFMP+GSLENHLFRRG+QPL+WA+R+KVAI AA+GL+FLH+A+
Sbjct: 181 VKLVGYCVEGENRLLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAIGAAKGLTFLHDAK 240

Query: 241 SPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
           S VIYRDFKA+NILLDAEFN+KLSDFGLAKAGPTGD+THV+TQVMGT GYAAPEY+ATG
Sbjct: 241 SQVIYRDFKAANILLDAEFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVATG 268

BLAST of Lsi08G007420.1 vs. ExPASy Swiss-Prot
Match: O49839 (Probable serine/threonine-protein kinase PBL2 OS=Arabidopsis thaliana OX=3702 GN=PBL2 PE=1 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 3.1e-103
Identity = 194/299 (64.88%), Postives = 226/299 (75.59%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSS---QGTSEISQSSSKKCHPLVPSNLPNSLADRNNNVLSLPTPK 60
           MGNCLDSSAKV N++ S      S  S+ SSK      PS L  +    +++   LPT +
Sbjct: 1   MGNCLDSSAKVDNSNHSPHANSASSGSKVSSKTSRSTGPSGLSTTSYSTDSSFGPLPTLR 60

Query: 61  TEGEILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMV 120
           TEGEILSS NLK FTFNELKNAT+NFR D+LLGEGGFG VFKGWID+ +L A+RPG G+V
Sbjct: 61  TEGEILSSPNLKAFTFNELKNATKNFRQDNLLGEGGFGCVFKGWIDQTSLTASRPGSGIV 120

Query: 121 VAVKKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNL 180
           VAVK+LKPE  QGHKEWL                            TEVNYLG+  HPNL
Sbjct: 121 VAVKQLKPEGFQGHKEWL----------------------------TEVNYLGQLSHPNL 180

Query: 181 VKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAE 240
           V L+GYC EGENRLLVYEFMP+GSLENHLFRRG+QPL+WA+R+KVA+ AA+GL+FLHEA+
Sbjct: 181 VLLVGYCAEGENRLLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAVGAAKGLTFLHEAK 240

Query: 241 SPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
           S VIYRDFKA+NILLDA+FNAKLSDFGLAKAGPTGD THV+T+V+GT GYAAPEY+ATG
Sbjct: 241 SQVIYRDFKAANILLDADFNAKLSDFGLAKAGPTGDNTHVSTKVIGTHGYAAPEYVATG 271

BLAST of Lsi08G007420.1 vs. ExPASy Swiss-Prot
Match: Q5XF79 (Probable serine/threonine-protein kinase PBL18 OS=Arabidopsis thaliana OX=3702 GN=PBL18 PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 4.9e-96
Identity = 189/300 (63.00%), Postives = 222/300 (74.00%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSS-QGTSEISQ---SSSKKCHPLVPSNLPNSLADRNNNVLSLPTP 60
           MGNCLDSSA+V N  S+  G+S IS+    SS+     +PS   NS    + + L   TP
Sbjct: 1   MGNCLDSSARVGNRESTFGGSSRISRKPNQSSRLSSLTIPSYSNNSFTTSSWSNL---TP 60

Query: 61  KTEGEILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGM 120
           ++EGE+L S  LK FTFNELK ATRNF+P+S++GEGGFG V+KGWI E +L+ ++PG GM
Sbjct: 61  RSEGELLPSPTLKAFTFNELKTATRNFKPNSMIGEGGFGCVYKGWIGERSLSPSKPGSGM 120

Query: 121 VVAVKKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPN 180
           VVAVKKLK E  QGHKEWL                            TEV+YLG+ HH N
Sbjct: 121 VVAVKKLKSEGFQGHKEWL----------------------------TEVHYLGRLHHMN 180

Query: 181 LVKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEA 240
           LVKLIGYCLEGE RLLVYE+MP+GSLENHLFRRG++P+ W  R+KVA +AARGLSFLHEA
Sbjct: 181 LVKLIGYCLEGEKRLLVYEYMPKGSLENHLFRRGAEPIPWKTRMKVAFSAARGLSFLHEA 240

Query: 241 ESPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
           +  VIYRDFKASNILLD +FNAKLSDFGLAKAGPTGDRTHVTTQV+GT+GYAAPEYIATG
Sbjct: 241 K--VIYRDFKASNILLDVDFNAKLSDFGLAKAGPTGDRTHVTTQVIGTQGYAAPEYIATG 267

BLAST of Lsi08G007420.1 vs. ExPASy Swiss-Prot
Match: Q5PP29 (Probable serine/threonine-protein kinase PBL4 OS=Arabidopsis thaliana OX=3702 GN=PBL4 PE=2 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 1.6e-94
Identity = 184/298 (61.74%), Postives = 220/298 (73.83%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSS-QGTSEISQSSSKKCHPLVPSNLPNSLADRNNNVLSLPTPKTE 60
           MGNC   SAKV N  S  +G+S IS   S+    L    + +S  + + +V SL TP++E
Sbjct: 1   MGNCFGFSAKVGNRESPYRGSSRISAKRSQSSR-LSSLTIQSSSYNDDTSVASLQTPRSE 60

Query: 61  GEILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMVVA 120
           GE+L+S  LK FTFNELK ATRNFRPDS++GEGGFGYV+KGWIDE TL+ ++PG GMVVA
Sbjct: 61  GELLASPTLKAFTFNELKTATRNFRPDSVIGEGGFGYVYKGWIDERTLSPSKPGSGMVVA 120

Query: 121 VKKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNLVK 180
           VKKLK E  QGH++WL                             EV+ LG+ HH NLVK
Sbjct: 121 VKKLKEEGFQGHRQWL----------------------------AEVDCLGRLHHMNLVK 180

Query: 181 LIGYCLEGEN-RLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAES 240
           LIGYC +G++ RLLVYE+MP+GSLENHLFRRG++P+ W  RIKVAI AARGL+FLHEA+ 
Sbjct: 181 LIGYCSKGDHIRLLVYEYMPKGSLENHLFRRGAEPIPWRTRIKVAIGAARGLAFLHEAQ- 240

Query: 241 PVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
            VIYRDFKASNILLD+EFNAKLSDFGLAK GPTGDRTHV+TQVMGT+GYAAPEY+ATG
Sbjct: 241 -VIYRDFKASNILLDSEFNAKLSDFGLAKVGPTGDRTHVSTQVMGTQGYAAPEYVATG 267

BLAST of Lsi08G007420.1 vs. ExPASy Swiss-Prot
Match: P46573 (Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 GN=PBL10 PE=1 SV=2)

HSP 1 Score: 339.0 bits (868), Expect = 5.6e-92
Identity = 179/298 (60.07%), Postives = 211/298 (70.81%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSSQGTSEISQSSSKKCHPLVPSNLPNSLADRNNNVLSLPTPKTEG 60
           MG CL +  K            +S  +S K    + S   +SL  ++++V     P+TEG
Sbjct: 1   MGICLSAQIKA-----------VSPGASPK---YMSSEANDSLGSKSSSVSIRTNPRTEG 60

Query: 61  EILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMVVAV 120
           EIL S NLK FTF ELK ATRNFRPDS+LGEGGFG VFKGWIDE TL A++PG G+V+AV
Sbjct: 61  EILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAV 120

Query: 121 KKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNLVKL 180
           KKL  +  QGH+EWL                             EVNYLG+F HPNLVKL
Sbjct: 121 KKLNQDGWQGHQEWL----------------------------AEVNYLGQFSHPNLVKL 180

Query: 181 IGYCLEGENRLLVYEFMPRGSLENHLFRRGS--QPLSWALRIKVAIAAARGLSFLHEAES 240
           IGYCLE E+RLLVYEFMPRGSLENHLFRRGS  QPLSW LR+KVA+ AA+GL+FLH AE+
Sbjct: 181 IGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAET 240

Query: 241 PVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
            VIYRDFK SNILLD+E+NAKLSDFGLAK GPTGD++HV+T++MGT GYAAPEY+ATG
Sbjct: 241 SVIYRDFKTSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATG 256

BLAST of Lsi08G007420.1 vs. ExPASy TrEMBL
Match: A0A5A7T4R2 (Protein kinase 2B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00870 PE=3 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 4.5e-137
Identity = 256/296 (86.49%), Postives = 261/296 (88.18%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSSQGTSEISQSSSKKCHPLVPSNLPNSLADRNNNVLSLPTPKTEG 60
           MGNCL SSAKVQNAH+SQGT EISQ+SSK+ HPLVPSNLPNSL DRN NV SLPTPKTEG
Sbjct: 1   MGNCLASSAKVQNAHTSQGTHEISQNSSKR-HPLVPSNLPNSLLDRNKNVPSLPTPKTEG 60

Query: 61  EILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMVVAV 120
           EILSSSNLKPF+FNEL+NATRNFRPDSLLGEGGFGYVFKGWIDENT AAARPGLGMVVAV
Sbjct: 61  EILSSSNLKPFSFNELRNATRNFRPDSLLGEGGFGYVFKGWIDENTWAAARPGLGMVVAV 120

Query: 121 KKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNLVKL 180
           KKLKPEASQGHKEWL                            TEVNYLGKFHHPNLVKL
Sbjct: 121 KKLKPEASQGHKEWL----------------------------TEVNYLGKFHHPNLVKL 180

Query: 181 IGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV 240
           IGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV
Sbjct: 181 IGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV 240

Query: 241 IYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
           IYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG
Sbjct: 241 IYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 267

BLAST of Lsi08G007420.1 vs. ExPASy TrEMBL
Match: A0A0A0KE20 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G404180 PE=3 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 4.5e-137
Identity = 253/296 (85.47%), Postives = 262/296 (88.51%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSSQGTSEISQSSSKKCHPLVPSNLPNSLADRNNNVLSLPTPKTEG 60
           MGNCLDSSAKVQNAH+SQGT EISQ+SSK+ HPLVPSNLPNSL DRNNNVLSLPTPKTEG
Sbjct: 1   MGNCLDSSAKVQNAHTSQGTHEISQNSSKR-HPLVPSNLPNSLVDRNNNVLSLPTPKTEG 60

Query: 61  EILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMVVAV 120
           EILSSSNLKPF+FNEL++ATRNFRPDSLLGEGGFGYVFKGWIDENT AA RPG+GMVVAV
Sbjct: 61  EILSSSNLKPFSFNELRSATRNFRPDSLLGEGGFGYVFKGWIDENTWAAVRPGMGMVVAV 120

Query: 121 KKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNLVKL 180
           KKLKPEASQGHKEWL                            TEVNYLGKFHHPNLVKL
Sbjct: 121 KKLKPEASQGHKEWL----------------------------TEVNYLGKFHHPNLVKL 180

Query: 181 IGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV 240
           IGYCLEGENRLLVYEF+PRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV
Sbjct: 181 IGYCLEGENRLLVYEFLPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV 240

Query: 241 IYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
           IYRDFKASNILLDAEFNAKLSDFGL KAGPTGDRTHVTTQV+GTRGYAAPEYIATG
Sbjct: 241 IYRDFKASNILLDAEFNAKLSDFGLVKAGPTGDRTHVTTQVIGTRGYAAPEYIATG 267

BLAST of Lsi08G007420.1 vs. ExPASy TrEMBL
Match: A0A1S3C2W0 (protein kinase 2B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103496405 PE=3 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 4.5e-137
Identity = 256/296 (86.49%), Postives = 261/296 (88.18%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSSQGTSEISQSSSKKCHPLVPSNLPNSLADRNNNVLSLPTPKTEG 60
           MGNCL SSAKVQNAH+SQGT EISQ+SSK+ HPLVPSNLPNSL DRN NV SLPTPKTEG
Sbjct: 1   MGNCLASSAKVQNAHTSQGTHEISQNSSKR-HPLVPSNLPNSLLDRNKNVPSLPTPKTEG 60

Query: 61  EILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMVVAV 120
           EILSSSNLKPF+FNEL+NATRNFRPDSLLGEGGFGYVFKGWIDENT AAARPGLGMVVAV
Sbjct: 61  EILSSSNLKPFSFNELRNATRNFRPDSLLGEGGFGYVFKGWIDENTWAAARPGLGMVVAV 120

Query: 121 KKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNLVKL 180
           KKLKPEASQGHKEWL                            TEVNYLGKFHHPNLVKL
Sbjct: 121 KKLKPEASQGHKEWL----------------------------TEVNYLGKFHHPNLVKL 180

Query: 181 IGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV 240
           IGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV
Sbjct: 181 IGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV 240

Query: 241 IYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
           IYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG
Sbjct: 241 IYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 267

BLAST of Lsi08G007420.1 vs. ExPASy TrEMBL
Match: A0A6J1KHV0 (probable serine/threonine-protein kinase PBL3 OS=Cucurbita maxima OX=3661 GN=LOC111493974 PE=3 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 2.2e-131
Identity = 249/298 (83.56%), Postives = 258/298 (86.58%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSSQGTSEISQSSSKKCHPLVPSNLPNSLADR--NNNVLSLPTPKT 60
           MGNC  SSAKV+ AHSS+ TSEIS+SSSKK +P+VPS  PNS+ADR  NNNVL+LPTP+T
Sbjct: 1   MGNCFGSSAKVEIAHSSRPTSEISRSSSKKHNPIVPSYNPNSVADRNDNNNVLNLPTPRT 60

Query: 61  EGEILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMVV 120
           EGEILSSSNLKPF FNELKNATRNFRPDSLLGEGGFGYVFKGWIDEN LAAARPGLGMVV
Sbjct: 61  EGEILSSSNLKPFHFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENKLAAARPGLGMVV 120

Query: 121 AVKKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNLV 180
           AVKKLK EASQGHKEWL                            TEV+YLGKFHHPNLV
Sbjct: 121 AVKKLKAEASQGHKEWL----------------------------TEVDYLGKFHHPNLV 180

Query: 181 KLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAES 240
           KLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAES
Sbjct: 181 KLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAES 240

Query: 241 PVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
           PVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG
Sbjct: 241 PVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 270

BLAST of Lsi08G007420.1 vs. ExPASy TrEMBL
Match: A0A6J1G338 (probable serine/threonine-protein kinase PBL3 OS=Cucurbita moschata OX=3662 GN=LOC111450344 PE=3 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 8.2e-131
Identity = 247/298 (82.89%), Postives = 257/298 (86.24%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSSQGTSEISQSSSKKCHPLVPSNLPNSLADR--NNNVLSLPTPKT 60
           MGNC  SSAKV+ AHSS+ TSEIS+SS KK +P+VPS  PNS+ADR  NNNVL+LPTP+T
Sbjct: 1   MGNCFGSSAKVEIAHSSRPTSEISRSSCKKHNPIVPSYYPNSVADRNDNNNVLNLPTPRT 60

Query: 61  EGEILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMVV 120
           EGEILSSSNLKPF FNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTL AARPGLGMVV
Sbjct: 61  EGEILSSSNLKPFHFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLGAARPGLGMVV 120

Query: 121 AVKKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNLV 180
           AVKKLK EASQGHKEWL                            TEV+YLGKFHHPNLV
Sbjct: 121 AVKKLKAEASQGHKEWL----------------------------TEVDYLGKFHHPNLV 180

Query: 181 KLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAES 240
           KLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEA+S
Sbjct: 181 KLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAKS 240

Query: 241 PVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
           PVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG
Sbjct: 241 PVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 270

BLAST of Lsi08G007420.1 vs. NCBI nr
Match: XP_038884960.1 (probable serine/threonine-protein kinase PBL3 [Benincasa hispida])

HSP 1 Score: 506.9 bits (1304), Expect = 1.2e-139
Identity = 259/296 (87.50%), Postives = 262/296 (88.51%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSSQGTSEISQSSSKKCHPLVPSNLPNSLADRNNNVLSLPTPKTEG 60
           MGNCL SSAKVQNAH+SQGT EISQSSSKK HPLVPSNLPNSL DRNNNVL LPTPKTEG
Sbjct: 1   MGNCLGSSAKVQNAHTSQGTLEISQSSSKKRHPLVPSNLPNSLVDRNNNVLGLPTPKTEG 60

Query: 61  EILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMVVAV 120
           EILSSSNLKPF+FNEL+NATRNFRPDSLLGEGGFGYVFKGWIDENT AAARPGLGMVVAV
Sbjct: 61  EILSSSNLKPFSFNELRNATRNFRPDSLLGEGGFGYVFKGWIDENTWAAARPGLGMVVAV 120

Query: 121 KKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNLVKL 180
           KKLKPEASQGHKEWL                            TEVNYLGKFHHPNLVKL
Sbjct: 121 KKLKPEASQGHKEWL----------------------------TEVNYLGKFHHPNLVKL 180

Query: 181 IGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV 240
           IGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV
Sbjct: 181 IGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV 240

Query: 241 IYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
           IYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG
Sbjct: 241 IYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 268

BLAST of Lsi08G007420.1 vs. NCBI nr
Match: XP_008456461.1 (PREDICTED: protein kinase 2B, chloroplastic-like [Cucumis melo] >KAA0036445.1 protein kinase 2B [Cucumis melo var. makuwa] >TYK00041.1 protein kinase 2B [Cucumis melo var. makuwa])

HSP 1 Score: 497.3 bits (1279), Expect = 9.3e-137
Identity = 256/296 (86.49%), Postives = 261/296 (88.18%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSSQGTSEISQSSSKKCHPLVPSNLPNSLADRNNNVLSLPTPKTEG 60
           MGNCL SSAKVQNAH+SQGT EISQ+SSK+ HPLVPSNLPNSL DRN NV SLPTPKTEG
Sbjct: 1   MGNCLASSAKVQNAHTSQGTHEISQNSSKR-HPLVPSNLPNSLLDRNKNVPSLPTPKTEG 60

Query: 61  EILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMVVAV 120
           EILSSSNLKPF+FNEL+NATRNFRPDSLLGEGGFGYVFKGWIDENT AAARPGLGMVVAV
Sbjct: 61  EILSSSNLKPFSFNELRNATRNFRPDSLLGEGGFGYVFKGWIDENTWAAARPGLGMVVAV 120

Query: 121 KKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNLVKL 180
           KKLKPEASQGHKEWL                            TEVNYLGKFHHPNLVKL
Sbjct: 121 KKLKPEASQGHKEWL----------------------------TEVNYLGKFHHPNLVKL 180

Query: 181 IGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV 240
           IGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV
Sbjct: 181 IGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV 240

Query: 241 IYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
           IYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG
Sbjct: 241 IYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 267

BLAST of Lsi08G007420.1 vs. NCBI nr
Match: XP_004146751.1 (probable serine/threonine-protein kinase PBL3 [Cucumis sativus])

HSP 1 Score: 497.3 bits (1279), Expect = 9.3e-137
Identity = 253/296 (85.47%), Postives = 262/296 (88.51%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSSQGTSEISQSSSKKCHPLVPSNLPNSLADRNNNVLSLPTPKTEG 60
           MGNCLDSSAKVQNAH+SQGT EISQ+SSK+ HPLVPSNLPNSL DRNNNVLSLPTPKTEG
Sbjct: 1   MGNCLDSSAKVQNAHTSQGTHEISQNSSKR-HPLVPSNLPNSLVDRNNNVLSLPTPKTEG 60

Query: 61  EILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMVVAV 120
           EILSSSNLKPF+FNEL++ATRNFRPDSLLGEGGFGYVFKGWIDENT AA RPG+GMVVAV
Sbjct: 61  EILSSSNLKPFSFNELRSATRNFRPDSLLGEGGFGYVFKGWIDENTWAAVRPGMGMVVAV 120

Query: 121 KKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNLVKL 180
           KKLKPEASQGHKEWL                            TEVNYLGKFHHPNLVKL
Sbjct: 121 KKLKPEASQGHKEWL----------------------------TEVNYLGKFHHPNLVKL 180

Query: 181 IGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV 240
           IGYCLEGENRLLVYEF+PRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV
Sbjct: 181 IGYCLEGENRLLVYEFLPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAESPV 240

Query: 241 IYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
           IYRDFKASNILLDAEFNAKLSDFGL KAGPTGDRTHVTTQV+GTRGYAAPEYIATG
Sbjct: 241 IYRDFKASNILLDAEFNAKLSDFGLVKAGPTGDRTHVTTQVIGTRGYAAPEYIATG 267

BLAST of Lsi08G007420.1 vs. NCBI nr
Match: XP_023522677.1 (probable serine/threonine-protein kinase PBL3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 478.8 bits (1231), Expect = 3.4e-131
Identity = 249/299 (83.28%), Postives = 257/299 (85.95%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSSQGTSEISQSSSKKCHPLVPSNLPNSLADR---NNNVLSLPTPK 60
           MGNC  SSAKV+ AHSS+ TSEISQSSSKK +P+VPS  PNS+ DR   NNNVL+LPTP+
Sbjct: 1   MGNCFGSSAKVEIAHSSRPTSEISQSSSKKHNPIVPSYNPNSVVDRNDNNNNVLNLPTPR 60

Query: 61  TEGEILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMV 120
           TEGEILSSSNLKPF FNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTL AARPGLGMV
Sbjct: 61  TEGEILSSSNLKPFHFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLGAARPGLGMV 120

Query: 121 VAVKKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNL 180
           VAVKKLK EASQGHKEWL                            TEV+YLGKFHHPNL
Sbjct: 121 VAVKKLKAEASQGHKEWL----------------------------TEVDYLGKFHHPNL 180

Query: 181 VKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAE 240
           VKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAE
Sbjct: 181 VKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAE 240

Query: 241 SPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
           SPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG
Sbjct: 241 SPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 271

BLAST of Lsi08G007420.1 vs. NCBI nr
Match: XP_022999699.1 (probable serine/threonine-protein kinase PBL3 [Cucurbita maxima])

HSP 1 Score: 478.4 bits (1230), Expect = 4.5e-131
Identity = 249/298 (83.56%), Postives = 258/298 (86.58%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSSQGTSEISQSSSKKCHPLVPSNLPNSLADR--NNNVLSLPTPKT 60
           MGNC  SSAKV+ AHSS+ TSEIS+SSSKK +P+VPS  PNS+ADR  NNNVL+LPTP+T
Sbjct: 1   MGNCFGSSAKVEIAHSSRPTSEISRSSSKKHNPIVPSYNPNSVADRNDNNNVLNLPTPRT 60

Query: 61  EGEILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMVV 120
           EGEILSSSNLKPF FNELKNATRNFRPDSLLGEGGFGYVFKGWIDEN LAAARPGLGMVV
Sbjct: 61  EGEILSSSNLKPFHFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENKLAAARPGLGMVV 120

Query: 121 AVKKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNLV 180
           AVKKLK EASQGHKEWL                            TEV+YLGKFHHPNLV
Sbjct: 121 AVKKLKAEASQGHKEWL----------------------------TEVDYLGKFHHPNLV 180

Query: 181 KLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAES 240
           KLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAES
Sbjct: 181 KLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAES 240

Query: 241 PVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
           PVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG
Sbjct: 241 PVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 270

BLAST of Lsi08G007420.1 vs. TAIR 10
Match: AT2G02800.1 (protein kinase 2B )

HSP 1 Score: 392.5 bits (1007), Expect = 3.0e-109
Identity = 204/299 (68.23%), Postives = 232/299 (77.59%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSSQGTSEISQS---SSKKCHPLVPSNLPNSLADRNNNVLSLPTPK 60
           MGNCLDSSAKV ++  S   +  S S   SSK     VPS+L     +  ++V SLPTP+
Sbjct: 1   MGNCLDSSAKVDSSSHSPHANSASLSSRVSSKTSRSTVPSSLS---INSYSSVESLPTPR 60

Query: 61  TEGEILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMV 120
           TEGEILSS NLK FTFNELKNATRNFRPDSLLGEGGFGYVFKGWID  TL A++PG G+V
Sbjct: 61  TEGEILSSPNLKAFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDGTTLTASKPGSGIV 120

Query: 121 VAVKKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNL 180
           VAVKKLK E  QGHKEWL                            TEVNYLG+  HPNL
Sbjct: 121 VAVKKLKTEGYQGHKEWL----------------------------TEVNYLGQLSHPNL 180

Query: 181 VKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAE 240
           VKL+GYC+EGENRLLVYEFMP+GSLENHLFRRG+QPL+WA+R+KVAI AA+GL+FLH+A+
Sbjct: 181 VKLVGYCVEGENRLLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAIGAAKGLTFLHDAK 240

Query: 241 SPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
           S VIYRDFKA+NILLDAEFN+KLSDFGLAKAGPTGD+THV+TQVMGT GYAAPEY+ATG
Sbjct: 241 SQVIYRDFKAANILLDAEFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVATG 268

BLAST of Lsi08G007420.1 vs. TAIR 10
Match: AT2G02800.2 (protein kinase 2B )

HSP 1 Score: 392.5 bits (1007), Expect = 3.0e-109
Identity = 204/299 (68.23%), Postives = 232/299 (77.59%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSSQGTSEISQS---SSKKCHPLVPSNLPNSLADRNNNVLSLPTPK 60
           MGNCLDSSAKV ++  S   +  S S   SSK     VPS+L     +  ++V SLPTP+
Sbjct: 1   MGNCLDSSAKVDSSSHSPHANSASLSSRVSSKTSRSTVPSSLS---INSYSSVESLPTPR 60

Query: 61  TEGEILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMV 120
           TEGEILSS NLK FTFNELKNATRNFRPDSLLGEGGFGYVFKGWID  TL A++PG G+V
Sbjct: 61  TEGEILSSPNLKAFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDGTTLTASKPGSGIV 120

Query: 121 VAVKKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNL 180
           VAVKKLK E  QGHKEWL                            TEVNYLG+  HPNL
Sbjct: 121 VAVKKLKTEGYQGHKEWL----------------------------TEVNYLGQLSHPNL 180

Query: 181 VKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAE 240
           VKL+GYC+EGENRLLVYEFMP+GSLENHLFRRG+QPL+WA+R+KVAI AA+GL+FLH+A+
Sbjct: 181 VKLVGYCVEGENRLLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAIGAAKGLTFLHDAK 240

Query: 241 SPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
           S VIYRDFKA+NILLDAEFN+KLSDFGLAKAGPTGD+THV+TQVMGT GYAAPEY+ATG
Sbjct: 241 SQVIYRDFKAANILLDAEFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVATG 268

BLAST of Lsi08G007420.1 vs. TAIR 10
Match: AT1G14370.1 (protein kinase 2A )

HSP 1 Score: 376.3 bits (965), Expect = 2.2e-104
Identity = 194/299 (64.88%), Postives = 226/299 (75.59%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSS---QGTSEISQSSSKKCHPLVPSNLPNSLADRNNNVLSLPTPK 60
           MGNCLDSSAKV N++ S      S  S+ SSK      PS L  +    +++   LPT +
Sbjct: 1   MGNCLDSSAKVDNSNHSPHANSASSGSKVSSKTSRSTGPSGLSTTSYSTDSSFGPLPTLR 60

Query: 61  TEGEILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMV 120
           TEGEILSS NLK FTFNELKNAT+NFR D+LLGEGGFG VFKGWID+ +L A+RPG G+V
Sbjct: 61  TEGEILSSPNLKAFTFNELKNATKNFRQDNLLGEGGFGCVFKGWIDQTSLTASRPGSGIV 120

Query: 121 VAVKKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNL 180
           VAVK+LKPE  QGHKEWL                            TEVNYLG+  HPNL
Sbjct: 121 VAVKQLKPEGFQGHKEWL----------------------------TEVNYLGQLSHPNL 180

Query: 181 VKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAE 240
           V L+GYC EGENRLLVYEFMP+GSLENHLFRRG+QPL+WA+R+KVA+ AA+GL+FLHEA+
Sbjct: 181 VLLVGYCAEGENRLLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAVGAAKGLTFLHEAK 240

Query: 241 SPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
           S VIYRDFKA+NILLDA+FNAKLSDFGLAKAGPTGD THV+T+V+GT GYAAPEY+ATG
Sbjct: 241 SQVIYRDFKAANILLDADFNAKLSDFGLAKAGPTGDNTHVSTKVIGTHGYAAPEYVATG 271

BLAST of Lsi08G007420.1 vs. TAIR 10
Match: AT1G69790.1 (Protein kinase superfamily protein )

HSP 1 Score: 352.4 bits (903), Expect = 3.4e-97
Identity = 189/300 (63.00%), Postives = 222/300 (74.00%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSS-QGTSEISQ---SSSKKCHPLVPSNLPNSLADRNNNVLSLPTP 60
           MGNCLDSSA+V N  S+  G+S IS+    SS+     +PS   NS    + + L   TP
Sbjct: 1   MGNCLDSSARVGNRESTFGGSSRISRKPNQSSRLSSLTIPSYSNNSFTTSSWSNL---TP 60

Query: 61  KTEGEILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGM 120
           ++EGE+L S  LK FTFNELK ATRNF+P+S++GEGGFG V+KGWI E +L+ ++PG GM
Sbjct: 61  RSEGELLPSPTLKAFTFNELKTATRNFKPNSMIGEGGFGCVYKGWIGERSLSPSKPGSGM 120

Query: 121 VVAVKKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPN 180
           VVAVKKLK E  QGHKEWL                            TEV+YLG+ HH N
Sbjct: 121 VVAVKKLKSEGFQGHKEWL----------------------------TEVHYLGRLHHMN 180

Query: 181 LVKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEA 240
           LVKLIGYCLEGE RLLVYE+MP+GSLENHLFRRG++P+ W  R+KVA +AARGLSFLHEA
Sbjct: 181 LVKLIGYCLEGEKRLLVYEYMPKGSLENHLFRRGAEPIPWKTRMKVAFSAARGLSFLHEA 240

Query: 241 ESPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
           +  VIYRDFKASNILLD +FNAKLSDFGLAKAGPTGDRTHVTTQV+GT+GYAAPEYIATG
Sbjct: 241 K--VIYRDFKASNILLDVDFNAKLSDFGLAKAGPTGDRTHVTTQVIGTQGYAAPEYIATG 267

BLAST of Lsi08G007420.1 vs. TAIR 10
Match: AT1G26970.1 (Protein kinase superfamily protein )

HSP 1 Score: 347.4 bits (890), Expect = 1.1e-95
Identity = 184/298 (61.74%), Postives = 220/298 (73.83%), Query Frame = 0

Query: 1   MGNCLDSSAKVQNAHSS-QGTSEISQSSSKKCHPLVPSNLPNSLADRNNNVLSLPTPKTE 60
           MGNC   SAKV N  S  +G+S IS   S+    L    + +S  + + +V SL TP++E
Sbjct: 1   MGNCFGFSAKVGNRESPYRGSSRISAKRSQSSR-LSSLTIQSSSYNDDTSVASLQTPRSE 60

Query: 61  GEILSSSNLKPFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLAAARPGLGMVVA 120
           GE+L+S  LK FTFNELK ATRNFRPDS++GEGGFGYV+KGWIDE TL+ ++PG GMVVA
Sbjct: 61  GELLASPTLKAFTFNELKTATRNFRPDSVIGEGGFGYVYKGWIDERTLSPSKPGSGMVVA 120

Query: 121 VKKLKPEASQGHKEWLVRSLVDIIFFFTFSVICLCLQLTAYKIQTEVNYLGKFHHPNLVK 180
           VKKLK E  QGH++WL                             EV+ LG+ HH NLVK
Sbjct: 121 VKKLKEEGFQGHRQWL----------------------------AEVDCLGRLHHMNLVK 180

Query: 181 LIGYCLEGEN-RLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAES 240
           LIGYC +G++ RLLVYE+MP+GSLENHLFRRG++P+ W  RIKVAI AARGL+FLHEA+ 
Sbjct: 181 LIGYCSKGDHIRLLVYEYMPKGSLENHLFRRGAEPIPWRTRIKVAIGAARGLAFLHEAQ- 240

Query: 241 PVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATG 297
            VIYRDFKASNILLD+EFNAKLSDFGLAK GPTGDRTHV+TQVMGT+GYAAPEY+ATG
Sbjct: 241 -VIYRDFKASNILLDSEFNAKLSDFGLAKVGPTGDRTHVSTQVMGTQGYAAPEYVATG 267

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O498404.2e-10868.23Probable serine/threonine-protein kinase PBL3 OS=Arabidopsis thaliana OX=3702 GN... [more]
O498393.1e-10364.88Probable serine/threonine-protein kinase PBL2 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q5XF794.9e-9663.00Probable serine/threonine-protein kinase PBL18 OS=Arabidopsis thaliana OX=3702 G... [more]
Q5PP291.6e-9461.74Probable serine/threonine-protein kinase PBL4 OS=Arabidopsis thaliana OX=3702 GN... [more]
P465735.6e-9260.07Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
A0A5A7T4R24.5e-13786.49Protein kinase 2B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00... [more]
A0A0A0KE204.5e-13785.47Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G404... [more]
A0A1S3C2W04.5e-13786.49protein kinase 2B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103496405 PE... [more]
A0A6J1KHV02.2e-13183.56probable serine/threonine-protein kinase PBL3 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1G3388.2e-13182.89probable serine/threonine-protein kinase PBL3 OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
XP_038884960.11.2e-13987.50probable serine/threonine-protein kinase PBL3 [Benincasa hispida][more]
XP_008456461.19.3e-13786.49PREDICTED: protein kinase 2B, chloroplastic-like [Cucumis melo] >KAA0036445.1 pr... [more]
XP_004146751.19.3e-13785.47probable serine/threonine-protein kinase PBL3 [Cucumis sativus][more]
XP_023522677.13.4e-13183.28probable serine/threonine-protein kinase PBL3 [Cucurbita pepo subsp. pepo][more]
XP_022999699.14.5e-13183.56probable serine/threonine-protein kinase PBL3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G02800.13.0e-10968.23protein kinase 2B [more]
AT2G02800.23.0e-10968.23protein kinase 2B [more]
AT1G14370.12.2e-10464.88protein kinase 2A [more]
AT1G69790.13.4e-9763.00Protein kinase superfamily protein [more]
AT1G26970.11.1e-9561.74Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 54..143
e-value: 1.0E-14
score: 56.4
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 156..197
e-value: 6.2E-13
score: 50.8
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 198..297
e-value: 3.3E-30
score: 107.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..49
NoneNo IPR availablePANTHERPTHR45621OS01G0588500 PROTEIN-RELATEDcoord: 163..296
coord: 20..136
NoneNo IPR availablePANTHERPTHR45621:SF35SERINE/THREONINE-PROTEIN KINASE PBL3-RELATEDcoord: 163..296
NoneNo IPR availablePANTHERPTHR45621:SF35SERINE/THREONINE-PROTEIN KINASE PBL3-RELATEDcoord: 20..136
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 161..294
e-value: 1.2E-27
score: 96.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 83..297
score: 31.325903
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 240..252
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 89..122
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 59..295

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi08G007420Lsi08G007420gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi08G007420.1.exon.4Lsi08G007420.1.exon.4exon
Lsi08G007420.1.exon.3Lsi08G007420.1.exon.3exon
Lsi08G007420.1.exon.2Lsi08G007420.1.exon.2exon
Lsi08G007420.1.exon.1Lsi08G007420.1.exon.1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi08G007420.1.CDS.4Lsi08G007420.1.CDS.4CDS
Lsi08G007420.1.CDS.3Lsi08G007420.1.CDS.3CDS
Lsi08G007420.1.CDS.2Lsi08G007420.1.CDS.2CDS
Lsi08G007420.1.CDS.1Lsi08G007420.1.CDS.1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi08G007420.1.five_prime_UTR.1Lsi08G007420.1.five_prime_UTR.1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi08G007420.1Lsi08G007420.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity