Lsi06G009890.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G009890.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	ENTH domain-containing protein
Location	chr06: 19921541 .. 19922383 (-)
Sequence length	843
RNA-Seq Expression	Lsi06G009890.1
Synteny	Lsi06G009890.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAAGAAGGCCTAAGGATTACAGTGAAATGAGAACGGAGGAGGAGATGAAGCTGAAGGCGCTACCGGTTAAGGAGTCTGGATACGACATGAATGAGATAAAGGCTCTTCCTCCGCTGGAGAATTGTGCTCCTGTTTCGCAATCGGTTTTGCGAATTCAATCGTTAAAGGAAGACTTGGTGAATTTGAGAGAGGACGGAGTATCAGCTGATGAGCAAGGGAACAAATTGGCCTTGGCTCTGTTCTCTGGAACCCCAATTACAAATCCTAATGGTTCATGGGAAACTTTCTCATCAAACCATGAGCCTGAAGTGAGTTCAGCGTGGCAGATGCCAGCTGCTGAGTTTGGCAAACCAGATTGGGAATTGGCATTGGTTGAAACTGCTAGCCATCTATCAAAACAGAAGCCTGATATGGGAGGTTGTTTTGATCCACTGCTATTAAATGGAATGTACGACCAAGCAACAGTTAGGCGACATGTCAATGCCGAGCAATTTGGGGGGAGTTCGAGTAGTGTGGCAATAGCATTGCAAGGCCAAGGCAATGCTGCTGCACAAGTTCTGGCACTTCCAGCACCAGATGGAACAGTTGAACCGATCGGGTATCGGGACCCTTTCGCTGCATCGCTTACGGTGCCGCCTCCTTCGTATGTGCAGATAGCTGAAATGAAGAAGAAGCAGCAGTTGCTAACGCAGGAGCAGCTTCTATGGAAGCAGTATGGCAGAGATGGAATGCAGGGCCAAGTGGGTTTGGGAAAACTTGGTGGTGGCTCGGCCTATGTTGGCCTAAATCCTCAGCAAATCATGCCTCATGGCTCAGCCTACTACTATGCTCCGTTTTGA

mRNA sequence

ATGTCAAGAAGGCCTAAGGATTACAGTGAAATGAGAACGGAGGAGGAGATGAAGCTGAAGGCGCTACCGGTTAAGGAGTCTGGATACGACATGAATGAGATAAAGGCTCTTCCTCCGCTGGAGAATTGTGCTCCTGTTTCGCAATCGGTTTTGCGAATTCAATCGTTAAAGGAAGACTTGGTGAATTTGAGAGAGGACGGAGTATCAGCTGATGAGCAAGGGAACAAATTGGCCTTGGCTCTGTTCTCTGGAACCCCAATTACAAATCCTAATGGTTCATGGGAAACTTTCTCATCAAACCATGAGCCTGAAGTGAGTTCAGCGTGGCAGATGCCAGCTGCTGAGTTTGGCAAACCAGATTGGGAATTGGCATTGGTTGAAACTGCTAGCCATCTATCAAAACAGAAGCCTGATATGGGAGGTTGTTTTGATCCACTGCTATTAAATGGAATGTACGACCAAGCAACAGTTAGGCGACATGTCAATGCCGAGCAATTTGGGGGGAGTTCGAGTAGTGTGGCAATAGCATTGCAAGGCCAAGGCAATGCTGCTGCACAAGTTCTGGCACTTCCAGCACCAGATGGAACAGTTGAACCGATCGGGTATCGGGACCCTTTCGCTGCATCGCTTACGGTGCCGCCTCCTTCGTATGTGCAGATAGCTGAAATGAAGAAGAAGCAGCAGTTGCTAACGCAGGAGCAGCTTCTATGGAAGCAGTATGGCAGAGATGGAATGCAGGGCCAAGTGGGTTTGGGAAAACTTGGTGGTGGCTCGGCCTATGTTGGCCTAAATCCTCAGCAAATCATGCCTCATGGCTCAGCCTACTACTATGCTCCGTTTTGA

Coding sequence (CDS)

ATGTCAAGAAGGCCTAAGGATTACAGTGAAATGAGAACGGAGGAGGAGATGAAGCTGAAGGCGCTACCGGTTAAGGAGTCTGGATACGACATGAATGAGATAAAGGCTCTTCCTCCGCTGGAGAATTGTGCTCCTGTTTCGCAATCGGTTTTGCGAATTCAATCGTTAAAGGAAGACTTGGTGAATTTGAGAGAGGACGGAGTATCAGCTGATGAGCAAGGGAACAAATTGGCCTTGGCTCTGTTCTCTGGAACCCCAATTACAAATCCTAATGGTTCATGGGAAACTTTCTCATCAAACCATGAGCCTGAAGTGAGTTCAGCGTGGCAGATGCCAGCTGCTGAGTTTGGCAAACCAGATTGGGAATTGGCATTGGTTGAAACTGCTAGCCATCTATCAAAACAGAAGCCTGATATGGGAGGTTGTTTTGATCCACTGCTATTAAATGGAATGTACGACCAAGCAACAGTTAGGCGACATGTCAATGCCGAGCAATTTGGGGGGAGTTCGAGTAGTGTGGCAATAGCATTGCAAGGCCAAGGCAATGCTGCTGCACAAGTTCTGGCACTTCCAGCACCAGATGGAACAGTTGAACCGATCGGGTATCGGGACCCTTTCGCTGCATCGCTTACGGTGCCGCCTCCTTCGTATGTGCAGATAGCTGAAATGAAGAAGAAGCAGCAGTTGCTAACGCAGGAGCAGCTTCTATGGAAGCAGTATGGCAGAGATGGAATGCAGGGCCAAGTGGGTTTGGGAAAACTTGGTGGTGGCTCGGCCTATGTTGGCCTAAATCCTCAGCAAATCATGCCTCATGGCTCAGCCTACTACTATGCTCCGTTTTGA

Protein sequence

MSRRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKALPPLENCAPVSQSVLRIQSLKEDLVNLREDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQMPAAEFGKPDWELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHVNAEQFGGSSSSVAIALQGQGNAAAQVLALPAPDGTVEPIGYRDPFAASLTVPPPSYVQIAEMKKKQQLLTQEQLLWKQYGRDGMQGQVGLGKLGGGSAYVGLNPQQIMPHGSAYYYAPF

Homology

[Close]

BLAST of Lsi06G009890.1 vs. ExPASy Swiss-Prot
Match: Q8LF20 (Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=At2g25430 PE=1 SV=2)

HSP 1 Score: 262.7 bits (670), Expect = 4.8e-69
Identity = 165/283 (58.30%), Postives = 190/283 (67.14%), Query Frame = 0


Query: 3   RRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKALPPLENCAPVSQSVLRIQSLK----E 62
           R  +  S  R E E     +  +E   DMNEIKALPP EN  P        Q  K    E
Sbjct: 376 RAKRGKSPERKEIEAPPPVVEEEEPEPDMNEIKALPPPENYTPPPPPEPEPQPEKPQFTE 435

Query: 63  DLVNLREDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQMPAAEFGK 122
           DLVNLRED V+AD+QGNK ALALF+G P    NG WE FSSN    V+SAWQ PAAE GK
Sbjct: 436 DLVNLREDEVTADDQGNKFALALFAGPP--GNNGKWEAFSSN---GVTSAWQNPAAEPGK 495

Query: 123 PDWELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHVNAEQF-GGSSSSVAIAL 182
            DWELALVET S+L KQ   +GG FD LLLNGMYDQ  VR+HV+  Q  GGS+SSVA+ L
Sbjct: 496 ADWELALVETTSNLEKQTAALGGGFDNLLLNGMYDQGMVRQHVSTSQLTGGSASSVALPL 555

Query: 183 QGQGNAAAQVLALPAPDGTVEPIGYRDPFAASLTVPPPSYVQIAEMKKKQQLLTQEQLLW 242
            G+ N   QVLALPAPDGTVE +  +DPFAASLT+PPPSYVQ+AEM+KKQ LL+QEQ LW
Sbjct: 556 PGKTN--NQVLALPAPDGTVEKVN-QDPFAASLTIPPPSYVQMAEMEKKQYLLSQEQQLW 615

Query: 243 KQYGRDGMQGQVGLGKLGGG--SAYVGLNPQQIM-PHGSAYYY 278
           +QY RDGM+GQ  L K+  G   AY G+ P   M P  + YYY
Sbjct: 616 QQYQRDGMRGQASLAKMNTGPVPAY-GMPPVNGMGPPPTGYYY 649

[Close]

BLAST of Lsi06G009890.1 vs. ExPASy Swiss-Prot
Match: Q8S9J8 (Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=At4g32285 PE=1 SV=2)

HSP 1 Score: 261.5 bits (667), Expect = 1.1e-68
Identity = 158/283 (55.83%), Postives = 188/283 (66.43%), Query Frame = 0


Query: 3   RRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKALPPLENCA----PVSQSVLRIQSLKE 62
           R  +  S  R E E      P  E   DMNEIKALPP EN      P  +   +   + +
Sbjct: 356 RAKRAKSPERKEIEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPAPEPKPQQPQVTD 415

Query: 63  DLVNLREDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQMPAAEFGK 122
           DLVNLRED VS D+QGNK ALALF+G P    NG WE FSS++   V+SAWQ PAAE GK
Sbjct: 416 DLVNLREDDVSGDDQGNKFALALFAGPPAN--NGKWEAFSSDN--NVTSAWQNPAAELGK 475

Query: 123 PDWELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHVN-AEQFGGSSSSVAIAL 182
            DWELALVETAS+L  QK  MGG  DPLLLNGMYDQ  VR+HV+ +E  GGSSSSVA+ L
Sbjct: 476 ADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELTGGSSSSVALPL 535

Query: 183 QGQGNAAAQVLALPAPDGTVEPIGYRDPFAASLTVPPPSYVQIAEMKKKQQLLTQEQLLW 242
            G+ N  + +LALPAPDGTV+ +  +DPFAASLT+PPPSYVQ+AEM KKQ LLTQEQ LW
Sbjct: 536 PGKVN--SHILALPAPDGTVQKVN-QDPFAASLTIPPPSYVQMAEMDKKQYLLTQEQQLW 595

Query: 243 KQYGRDGMQGQVGLGKLGGGSAYV--GLNPQQIM-PHGSAYYY 278
           +QY ++GM+GQ  L K+      +  G+ P   M P    YYY
Sbjct: 596 QQYQQEGMRGQASLAKMNTAQTAMPYGMPPVNGMGPSPMGYYY 631

[Close]

BLAST of Lsi06G009890.1 vs. ExPASy Swiss-Prot
Match: Q9SA65 (Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=At1g03050 PE=2 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 6.5e-34
Identity = 108/268 (40.30%), Postives = 145/268 (54.10%), Query Frame = 0


Query: 2   SRRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKAL---PPLE--NCAPVSQS-----VL 61
           S   +D  E RTEE        V E   DMN IKAL   PP E  +  P  ++     + 
Sbjct: 339 SEADEDDDEARTEE--------VNEEQEDMNAIKALPEPPPKEEDDVKPEEEAKEEVIIE 398

Query: 62  RIQSLKEDLVNL-REDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQ 121
           + Q    DL++L   +G  A + G+ LALALF G P  + +GS          E    W+
Sbjct: 399 KKQEEMGDLLDLGNTNGGEAGQAGDSLALALFDG-PYASGSGS----------ESGPGWE 458

Query: 122 MPAAEFGKPDWELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHV-NAEQFGGS 181
             A +    DWE ALV+TA++LS QK ++GG FD LLLNGMY    V   V  +  +G S
Sbjct: 459 --AFKDDSADWETALVQTATNLSGQKSELGGGFDMLLLNGMYQHGAVNAAVKTSTAYGAS 518

Query: 182 SSSVAIALQGQGNAAAQVLALPAPD------GTVEPIGYRDPFAASLTVPPPSYVQIAEM 241
            S+ ++A    G  AA +LALPAP       G +      DPFAASL V PP+YVQ+ +M
Sbjct: 519 GSASSMAFGSAGRPAATMLALPAPSTANGNAGNINSPVPMDPFAASLEVAPPAYVQMNDM 578

Query: 242 KKKQQLLTQEQLLWKQYGRDGMQGQVGL 252
           +KKQ++L +EQ++W QY RDG QG + L
Sbjct: 579 EKKQRMLMEEQMMWDQYSRDGRQGHMNL 585

[Close]

BLAST of Lsi06G009890.1 vs. ExPASy Swiss-Prot
Match: Q8GX47 (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 2.5e-33
Identity = 102/261 (39.08%), Postives = 139/261 (53.26%), Query Frame = 0


Query: 15  EEMKLKALPVKESGYDMNEIKALP------------PLENCAPVSQSVLRIQSLKEDLVN 74
           EE + K   ++E+  D+N IKALP             +E    V + V R Q  + DL++
Sbjct: 349 EEEESKTEYIQENQEDLNSIKALPAPEQKEEEEEEEKMETKKDVEEVVSR-QDQEGDLLD 408

Query: 75  LRED-GVSADEQGNKLALALFSGTPITN--PNGSWETFSSNHEPEVSSAWQMPAAEFGKP 134
           L ++ GV+A   G+ LALALF G   T       WE F+ N                   
Sbjct: 409 LTDEAGVTAGTVGDSLALALFDGVVGTESASGPGWEAFNDN-----------------SA 468

Query: 135 DWELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHV-NAEQFGGSSSSVAIALQ 194
           DWE  LV +A+ LS QK ++GG FD LLL+GMY    V   V  +  +G S S+ ++A  
Sbjct: 469 DWETDLVRSATRLSGQKSELGGGFDTLLLDGMYQYGAVNAAVKTSTAYGSSGSASSVAFG 528

Query: 195 GQGNAAAQVLALPAPDGTVEPIGYR------DPFAASLTVPPPSYVQIAEMKKKQQLLTQ 254
             G+ AA +LALPAP  T    G R      DPFAASL V PP+YVQ+ +M+KKQ+LL +
Sbjct: 529 SAGSPAASMLALPAPPPTAN--GNRNSPVMVDPFAASLEVAPPAYVQMNDMEKKQRLLME 588

[Close]

BLAST of Lsi06G009890.1 vs. ExPASy Swiss-Prot
Match: Q9LVD8 (Putative clathrin assembly protein At5g57200 OS=Arabidopsis thaliana OX=3702 GN=At5g57200 PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.6e-06
Identity = 64/245 (26.12%), Postives = 99/245 (40.41%), Query Frame = 0


Query: 6   KDYSEMRTEEEMKLKALPVKESGYDMNEIKALPPLENCAPVSQSVLRIQSLK-------E 65
           K+  E   EEE   +  P +E   + N     P +E      +  + ++  K       +
Sbjct: 324 KEEEEQEQEEEQPEE--PAEEENQNENTENDQPLIEEEEEEPKEEIEVEEAKPSPLIDTD 383

Query: 66  DLVNLREDGVSAD--EQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQMPAAEF 125
           DL+ L E    A   EQ N  +LA++       P G   +  SN          +   E 
Sbjct: 384 DLLGLHEINPKAAEIEQNNAFSLAIY-------PPGHETSAPSN---------SLSLIEA 443

Query: 126 GKPDWELALVETASHLSKQK-------PDMGGCFDPLLLNGMYDQATVRRHVNAEQFGGS 185
           G   WELALV   ++ +            +GG FD LLL+ +Y+  T RR +     G  
Sbjct: 444 GGSGWELALVTPQNNNNNNNNPRPVIATKLGGGFDNLLLDSLYEDDTARRQIQLTNAGYG 503

Query: 186 SSSVAIALQGQGNAAAQVLALPAPDGTVEPIGYRDPFAASLTVPPPSYVQIAEMKKKQQL 235
             + AI         A   + P P G       +DPFA S  + PP+ VQ+A M+++Q +
Sbjct: 504 FGATAI-------PGALASSNPNPFGV-----QQDPFAMSNNMAPPTNVQMA-MQQQQMM 537

[Close]

BLAST of Lsi06G009890.1 vs. ExPASy TrEMBL
Match: A0A5D3C4X9 (Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G00870 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 3.5e-115
Identity = 217/260 (83.46%), Postives = 231/260 (88.85%), Query Frame = 0


Query: 1   MSRRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKALPPLENCAPVSQSVLRIQSLKEDL 60
           M+RRPKDY+EMRT+E   +K + VKES YDMNEIKALPP ENCAPVSQSVL  QSLKEDL
Sbjct: 306 MTRRPKDYNEMRTKE---MKPVSVKESRYDMNEIKALPPPENCAPVSQSVL--QSLKEDL 365

Query: 61  VNLREDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQMPAAEFGKPD 120
           VNLRED VSADEQG KLA+A FSG P TNPNGSWETFS NHEPEVSS WQ  AAEFGKPD
Sbjct: 366 VNLREDEVSADEQGEKLAMAFFSGPPTTNPNGSWETFSLNHEPEVSSVWQTRAAEFGKPD 425

Query: 121 WELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHVNAEQFGGSSSSVAIALQGQ 180
           WELALVETASHLSKQ+PDMGGCFDP+LLNGMYDQ  VRRHVN EQ GGSSSSVA+ALQGQ
Sbjct: 426 WELALVETASHLSKQRPDMGGCFDPMLLNGMYDQEAVRRHVNVEQLGGSSSSVAVALQGQ 485

Query: 181 GNAAAQVLALPAPDGTVEPIGYRDPFAASLTVPPPSYVQIAEMKKKQQLLTQEQLLWKQY 240
           G+A +QVLALPAPDGTVE +G RDPFAASL VPPPSYVQIAEM+KKQQLL QEQLLWKQY
Sbjct: 486 GSAGSQVLALPAPDGTVELVGQRDPFAASLMVPPPSYVQIAEMEKKQQLLAQEQLLWKQY 545

Query: 241 GRDGMQGQVGLGKLGGGSAY 261
           GRDGMQGQVGL K+G GSAY
Sbjct: 546 GRDGMQGQVGLEKVGNGSAY 560

[Close]

BLAST of Lsi06G009890.1 vs. ExPASy TrEMBL
Match: A0A1S3B992 (putative clathrin assembly protein At2g25430 OS=Cucumis melo OX=3656 GN=LOC103487428 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 3.5e-115
Identity = 217/260 (83.46%), Postives = 231/260 (88.85%), Query Frame = 0


Query: 1   MSRRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKALPPLENCAPVSQSVLRIQSLKEDL 60
           M+RRPKDY+EMRT+E   +K + VKES YDMNEIKALPP ENCAPVSQSVL  QSLKEDL
Sbjct: 306 MTRRPKDYNEMRTKE---MKPVSVKESRYDMNEIKALPPPENCAPVSQSVL--QSLKEDL 365

Query: 61  VNLREDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQMPAAEFGKPD 120
           VNLRED VSADEQG KLA+A FSG P TNPNGSWETFS NHEPEVSS WQ  AAEFGKPD
Sbjct: 366 VNLREDEVSADEQGEKLAMAFFSGPPTTNPNGSWETFSLNHEPEVSSVWQTRAAEFGKPD 425

Query: 121 WELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHVNAEQFGGSSSSVAIALQGQ 180
           WELALVETASHLSKQ+PDMGGCFDP+LLNGMYDQ  VRRHVN EQ GGSSSSVA+ALQGQ
Sbjct: 426 WELALVETASHLSKQRPDMGGCFDPMLLNGMYDQEAVRRHVNVEQLGGSSSSVAVALQGQ 485

Query: 181 GNAAAQVLALPAPDGTVEPIGYRDPFAASLTVPPPSYVQIAEMKKKQQLLTQEQLLWKQY 240
           G+A +QVLALPAPDGTVE +G RDPFAASL VPPPSYVQIAEM+KKQQLL QEQLLWKQY
Sbjct: 486 GSAGSQVLALPAPDGTVELVGQRDPFAASLMVPPPSYVQIAEMEKKQQLLAQEQLLWKQY 545

Query: 241 GRDGMQGQVGLGKLGGGSAY 261
           GRDGMQGQVGL K+G GSAY
Sbjct: 546 GRDGMQGQVGLEKVGNGSAY 560

[Close]

BLAST of Lsi06G009890.1 vs. ExPASy TrEMBL
Match: A0A6J1F0M3 (probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LOC111441244 PE=4 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 1.3e-109
Identity = 215/280 (76.79%), Postives = 235/280 (83.93%), Query Frame = 0


Query: 1   MSRRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKALPPLENCAPVSQSVLRIQSLKEDL 60
           MSRRPKDY+E R EE  K+K+LP +E    MNEIKALPPLENC+P     L+  S  EDL
Sbjct: 315 MSRRPKDYNERRREE--KVKSLPAEEPECYMNEIKALPPLENCSP----TLQPPSRPEDL 374

Query: 61  VNLREDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQMPAAEFGKPD 120
           VNLREDGVSADEQGNKLALALFSG P  NPNGSWETFSS+ +PEVSSAWQ PAAE GKP+
Sbjct: 375 VNLREDGVSADEQGNKLALALFSGPP-ANPNGSWETFSSDKQPEVSSAWQTPAAEAGKPE 434

Query: 121 WELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHVNAEQFGGSSSSVAIALQGQ 180
           WELALVETAS+LSKQK DMGG FDPLLLNGMYD  T+R+H NAEQF GSSSSV  A++GQ
Sbjct: 435 WELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRQHANAEQFDGSSSSV--AMKGQ 494

Query: 181 GNAAAQVLALPAPDGTVEPIGYRDPFAASLTVPPPSYVQIAEMKKKQQLLTQEQLLWKQY 240
              AAQVLALPAPDGTV+PIG +DPFAASLTVPPPSYVQIAEM+KKQQLL QEQLLW+QY
Sbjct: 495 SKTAAQVLALPAPDGTVKPIGDQDPFAASLTVPPPSYVQIAEMEKKQQLLMQEQLLWQQY 554

Query: 241 GRDGMQGQVGLGKLGGGSAYVGLNPQQIMPHGSAYYYAPF 281
           GRDGM GQVGL KL GGSAY+G NPQ I P  SAY+YAPF
Sbjct: 555 GRDGMHGQVGLVKLAGGSAYIGSNPQPIAPQSSAYHYAPF 585

[Close]

BLAST of Lsi06G009890.1 vs. ExPASy TrEMBL
Match: A0A6J1I3H3 (probable clathrin assembly protein At4g32285 OS=Cucurbita maxima OX=3661 GN=LOC111470668 PE=4 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 2.4e-108
Identity = 212/280 (75.71%), Postives = 232/280 (82.86%), Query Frame = 0


Query: 1   MSRRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKALPPLENCAPVSQSVLRIQSLKEDL 60
           MSRRPKDY+E R EE  K+K+LP +E    MNEIKALPPLENC+P     L+  S  EDL
Sbjct: 311 MSRRPKDYNERRREE--KVKSLPTEEPECYMNEIKALPPLENCSP----TLQPPSRPEDL 370

Query: 61  VNLREDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQMPAAEFGKPD 120
           VNLREDGVSADEQGNKLA ALFSG P  NPNGSWETFSS+ +PEVSSAWQ PAAE GKP+
Sbjct: 371 VNLREDGVSADEQGNKLAWALFSGPP-ANPNGSWETFSSDKQPEVSSAWQTPAAEAGKPE 430

Query: 121 WELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHVNAEQFGGSSSSVAIALQGQ 180
           WELALVETAS+LSKQK DMGG FDPLLLNGMYD  T+R+H N EQFGGSSSSV  AL+GQ
Sbjct: 431 WELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRQHANGEQFGGSSSSV--ALKGQ 490

Query: 181 GNAAAQVLALPAPDGTVEPIGYRDPFAASLTVPPPSYVQIAEMKKKQQLLTQEQLLWKQY 240
           G  AAQVLALPAPDGTV+P+G +DPFA SLTVPPPSYVQIAEM+KKQQLL QEQLLW+QY
Sbjct: 491 GKTAAQVLALPAPDGTVKPVGVQDPFAVSLTVPPPSYVQIAEMEKKQQLLMQEQLLWQQY 550

Query: 241 GRDGMQGQVGLGKLGGGSAYVGLNPQQIMPHGSAYYYAPF 281
           G DGM GQVGL KL GGS Y+G NPQ I P  SAY+YAPF
Sbjct: 551 GIDGMHGQVGLVKLAGGSVYIGPNPQPIAPQSSAYHYAPF 581

[Close]

BLAST of Lsi06G009890.1 vs. ExPASy TrEMBL
Match: A0A6J1CV16 (probable clathrin assembly protein At4g32285 OS=Momordica charantia OX=3673 GN=LOC111014605 PE=4 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 2.7e-107
Identity = 211/281 (75.09%), Postives = 235/281 (83.63%), Query Frame = 0


Query: 1   MSRRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKALPPLENCAPVSQSVLRIQSL-KED 60
           M RRPKD++E R EE++KL   P  +   DMNE+KALPP +N  P   SVL    L KED
Sbjct: 313 MGRRPKDFNERRREEKLKLP--PADDPEQDMNEMKALPPPKNHDPSLHSVLPQTRLPKED 372

Query: 61  LVNLREDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQMPAAEFGKP 120
           LVNLRED VSADEQGNKLALALFSG PITNP+GSWETFSSN +PEVSSAW+ PAAE GK 
Sbjct: 373 LVNLREDEVSADEQGNKLALALFSGPPITNPDGSWETFSSNQDPEVSSAWKTPAAEAGKA 432

Query: 121 DWELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHVNAEQFGGSSSSVAIALQG 180
           DWELALVETAS+LSKQK DMGG FDPLLLN MYDQ T+RRHVNAEQFGGSSSSVA+ +QG
Sbjct: 433 DWELALVETASNLSKQKADMGGGFDPLLLNAMYDQGTIRRHVNAEQFGGSSSSVALQVQG 492

Query: 181 QGNAAAQVLALPAPDGTVEPIGYRDPFAASLTVPPPSYVQIAEMKKKQQLLTQEQLLWKQ 240
           +   AAQVLALPAPDGTV P+G+RDPFAASLTVPPPSYVQIAEM+KKQQLL QEQL+W+Q
Sbjct: 493 K-TXAAQVLALPAPDGTVTPVGHRDPFAASLTVPPPSYVQIAEMEKKQQLLVQEQLVWQQ 552

Query: 241 YGRDGMQGQVGLGKLGGGSAYVGLNPQQIMPHGSAYYYAPF 281
           YGR+GM GQVGL K+GGGSA+V +    IMPH SAYYYAPF
Sbjct: 553 YGREGMHGQVGLAKIGGGSAHVNV---AIMPHASAYYYAPF 587

[Close]

BLAST of Lsi06G009890.1 vs. NCBI nr
Match: XP_038879765.1 (putative clathrin assembly protein At2g25430 [Benincasa hispida])

HSP 1 Score: 509.2 bits (1310), Expect = 2.2e-140
Identity = 257/280 (91.79%), Postives = 262/280 (93.57%), Query Frame = 0


Query: 1   MSRRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKALPPLENCAPVSQSVLRIQSLKEDL 60
           MSRRPKD+ EMRTEEEM  K LPV ESGYDMNEIKALP LENCAPV QS+LR QS KEDL
Sbjct: 306 MSRRPKDFDEMRTEEEM--KPLPVNESGYDMNEIKALPLLENCAPVPQSILRTQSPKEDL 365

Query: 61  VNLREDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQMPAAEFGKPD 120
           VNLREDGVSADEQGNKLALALFSG PITNPNGSWETFSSNHEPEVSSAWQ PAAEFGKPD
Sbjct: 366 VNLREDGVSADEQGNKLALALFSGPPITNPNGSWETFSSNHEPEVSSAWQTPAAEFGKPD 425

Query: 121 WELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHVNAEQFGGSSSSVAIALQGQ 180
           WEL LVETASHLSKQKPDMGGCFDPLLLNGMYDQ TVRRHVNA QFGGSSSSVA+ALQGQ
Sbjct: 426 WELTLVETASHLSKQKPDMGGCFDPLLLNGMYDQETVRRHVNAVQFGGSSSSVAVALQGQ 485

Query: 181 GNAAAQVLALPAPDGTVEPIGYRDPFAASLTVPPPSYVQIAEMKKKQQLLTQEQLLWKQY 240
           GNAAAQVLALPAPDGT EPIGYRDPFAASLTVPPPSYVQIAEM+KKQ+LLTQEQLLWKQY
Sbjct: 486 GNAAAQVLALPAPDGTAEPIGYRDPFAASLTVPPPSYVQIAEMEKKQELLTQEQLLWKQY 545

Query: 241 GRDGMQGQVGLGKLGGGSAYVGLNPQQIMPHGSAYYYAPF 281
           GRDGMQGQ GLGKLGGGSAYV LNPQQIMPHGSAYYYA F
Sbjct: 546 GRDGMQGQGGLGKLGGGSAYVDLNPQQIMPHGSAYYYASF 583

[Close]

BLAST of Lsi06G009890.1 vs. NCBI nr
Match: XP_008443967.1 (PREDICTED: putative clathrin assembly protein At2g25430 [Cucumis melo] >TYK06390.1 putative clathrin assembly protein [Cucumis melo var. makuwa])

HSP 1 Score: 424.5 bits (1090), Expect = 7.2e-115
Identity = 217/260 (83.46%), Postives = 231/260 (88.85%), Query Frame = 0


Query: 1   MSRRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKALPPLENCAPVSQSVLRIQSLKEDL 60
           M+RRPKDY+EMRT+E   +K + VKES YDMNEIKALPP ENCAPVSQSVL  QSLKEDL
Sbjct: 306 MTRRPKDYNEMRTKE---MKPVSVKESRYDMNEIKALPPPENCAPVSQSVL--QSLKEDL 365

Query: 61  VNLREDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQMPAAEFGKPD 120
           VNLRED VSADEQG KLA+A FSG P TNPNGSWETFS NHEPEVSS WQ  AAEFGKPD
Sbjct: 366 VNLREDEVSADEQGEKLAMAFFSGPPTTNPNGSWETFSLNHEPEVSSVWQTRAAEFGKPD 425

Query: 121 WELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHVNAEQFGGSSSSVAIALQGQ 180
           WELALVETASHLSKQ+PDMGGCFDP+LLNGMYDQ  VRRHVN EQ GGSSSSVA+ALQGQ
Sbjct: 426 WELALVETASHLSKQRPDMGGCFDPMLLNGMYDQEAVRRHVNVEQLGGSSSSVAVALQGQ 485

Query: 181 GNAAAQVLALPAPDGTVEPIGYRDPFAASLTVPPPSYVQIAEMKKKQQLLTQEQLLWKQY 240
           G+A +QVLALPAPDGTVE +G RDPFAASL VPPPSYVQIAEM+KKQQLL QEQLLWKQY
Sbjct: 486 GSAGSQVLALPAPDGTVELVGQRDPFAASLMVPPPSYVQIAEMEKKQQLLAQEQLLWKQY 545

Query: 241 GRDGMQGQVGLGKLGGGSAY 261
           GRDGMQGQVGL K+G GSAY
Sbjct: 546 GRDGMQGQVGLEKVGNGSAY 560

[Close]

BLAST of Lsi06G009890.1 vs. NCBI nr
Match: KAG6587890.1 (putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 408.3 bits (1048), Expect = 5.4e-110
Identity = 216/280 (77.14%), Postives = 236/280 (84.29%), Query Frame = 0


Query: 1   MSRRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKALPPLENCAPVSQSVLRIQSLKEDL 60
           MSRRPKDY+E R EE  K+K+LP +E    MNEIKALPPLENC+P     L+  S  EDL
Sbjct: 315 MSRRPKDYNERRREE--KVKSLPAEEPECYMNEIKALPPLENCSP----TLQPPSRPEDL 374

Query: 61  VNLREDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQMPAAEFGKPD 120
           VNLREDGVSADEQGNKLALALFSG P  NPNGSWETFSS+ +PEVSSAWQ PAAE GKP+
Sbjct: 375 VNLREDGVSADEQGNKLALALFSGPP-ANPNGSWETFSSDKQPEVSSAWQTPAAEAGKPE 434

Query: 121 WELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHVNAEQFGGSSSSVAIALQGQ 180
           WELALVETAS+LSKQK DMGG FDPLLLNGMYD  T+R+H NAEQF GSSSSV  A++GQ
Sbjct: 435 WELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRQHANAEQFDGSSSSV--AMKGQ 494

Query: 181 GNAAAQVLALPAPDGTVEPIGYRDPFAASLTVPPPSYVQIAEMKKKQQLLTQEQLLWKQY 240
           G  AAQVLALPAPDGTV+PIG +DPFAASLTVPPPSYVQIAEM+KKQQLL QEQLLW+QY
Sbjct: 495 GKTAAQVLALPAPDGTVKPIGDQDPFAASLTVPPPSYVQIAEMEKKQQLLMQEQLLWQQY 554

Query: 241 GRDGMQGQVGLGKLGGGSAYVGLNPQQIMPHGSAYYYAPF 281
           GRDGM GQVGL KL GGSAY+G NPQ I P  SAY+YAPF
Sbjct: 555 GRDGMHGQVGLVKLAGGSAYIGSNPQPIAPQSSAYHYAPF 585

[Close]

BLAST of Lsi06G009890.1 vs. NCBI nr
Match: XP_023529315.1 (putative clathrin assembly protein At2g25430 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 406.4 bits (1043), Expect = 2.0e-109
Identity = 216/280 (77.14%), Postives = 235/280 (83.93%), Query Frame = 0


Query: 1   MSRRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKALPPLENCAPVSQSVLRIQSLKEDL 60
           MSRRPKDY+E R EE  K+K+LP +E    MNEIKALPPLENC+P     L+  S   DL
Sbjct: 315 MSRRPKDYNERRREE--KVKSLPAEEPECYMNEIKALPPLENCSP----TLQPPSRPIDL 374

Query: 61  VNLREDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQMPAAEFGKPD 120
           VNLREDGVSADEQGNKLALALFSG P  NPNGSWETFSS+ +PEVSSAWQ PAAE GKP+
Sbjct: 375 VNLREDGVSADEQGNKLALALFSGPP-ANPNGSWETFSSDKQPEVSSAWQTPAAEAGKPE 434

Query: 121 WELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHVNAEQFGGSSSSVAIALQGQ 180
           WELALVETAS+LSKQK DMGG FDPLLLNGMYD  T+R+H NAEQFGGSSSSV  AL+GQ
Sbjct: 435 WELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRKHANAEQFGGSSSSV--ALKGQ 494

Query: 181 GNAAAQVLALPAPDGTVEPIGYRDPFAASLTVPPPSYVQIAEMKKKQQLLTQEQLLWKQY 240
           G  AAQVLALPAPDGTV+PIG +DPFAASLTVPPPSYVQIAEM+ KQQLL QEQLLW+QY
Sbjct: 495 GKTAAQVLALPAPDGTVKPIGDQDPFAASLTVPPPSYVQIAEMEMKQQLLMQEQLLWQQY 554

Query: 241 GRDGMQGQVGLGKLGGGSAYVGLNPQQIMPHGSAYYYAPF 281
           GRDGM GQVGL KL GGSAY+G NPQ I P  SAY+YAPF
Sbjct: 555 GRDGMHGQVGLVKLAGGSAYIGPNPQPIAPQSSAYHYAPF 585

[Close]

BLAST of Lsi06G009890.1 vs. NCBI nr
Match: XP_022934011.1 (probable clathrin assembly protein At4g32285 [Cucurbita moschata])

HSP 1 Score: 406.0 bits (1042), Expect = 2.7e-109
Identity = 215/280 (76.79%), Postives = 235/280 (83.93%), Query Frame = 0


Query: 1   MSRRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKALPPLENCAPVSQSVLRIQSLKEDL 60
           MSRRPKDY+E R EE  K+K+LP +E    MNEIKALPPLENC+P     L+  S  EDL
Sbjct: 315 MSRRPKDYNERRREE--KVKSLPAEEPECYMNEIKALPPLENCSP----TLQPPSRPEDL 374

Query: 61  VNLREDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQMPAAEFGKPD 120
           VNLREDGVSADEQGNKLALALFSG P  NPNGSWETFSS+ +PEVSSAWQ PAAE GKP+
Sbjct: 375 VNLREDGVSADEQGNKLALALFSGPP-ANPNGSWETFSSDKQPEVSSAWQTPAAEAGKPE 434

Query: 121 WELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHVNAEQFGGSSSSVAIALQGQ 180
           WELALVETAS+LSKQK DMGG FDPLLLNGMYD  T+R+H NAEQF GSSSSV  A++GQ
Sbjct: 435 WELALVETASNLSKQKADMGGGFDPLLLNGMYDHGTIRQHANAEQFDGSSSSV--AMKGQ 494

Query: 181 GNAAAQVLALPAPDGTVEPIGYRDPFAASLTVPPPSYVQIAEMKKKQQLLTQEQLLWKQY 240
              AAQVLALPAPDGTV+PIG +DPFAASLTVPPPSYVQIAEM+KKQQLL QEQLLW+QY
Sbjct: 495 SKTAAQVLALPAPDGTVKPIGDQDPFAASLTVPPPSYVQIAEMEKKQQLLMQEQLLWQQY 554

Query: 241 GRDGMQGQVGLGKLGGGSAYVGLNPQQIMPHGSAYYYAPF 281
           GRDGM GQVGL KL GGSAY+G NPQ I P  SAY+YAPF
Sbjct: 555 GRDGMHGQVGLVKLAGGSAYIGSNPQPIAPQSSAYHYAPF 585

[Close]

BLAST of Lsi06G009890.1 vs. TAIR 10
Match: AT2G25430.1 (epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related )

HSP 1 Score: 262.7 bits (670), Expect = 3.4e-70
Identity = 165/283 (58.30%), Postives = 190/283 (67.14%), Query Frame = 0


Query: 3   RRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKALPPLENCAPVSQSVLRIQSLK----E 62
           R  +  S  R E E     +  +E   DMNEIKALPP EN  P        Q  K    E
Sbjct: 376 RAKRGKSPERKEIEAPPPVVEEEEPEPDMNEIKALPPPENYTPPPPPEPEPQPEKPQFTE 435

Query: 63  DLVNLREDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQMPAAEFGK 122
           DLVNLRED V+AD+QGNK ALALF+G P    NG WE FSSN    V+SAWQ PAAE GK
Sbjct: 436 DLVNLREDEVTADDQGNKFALALFAGPP--GNNGKWEAFSSN---GVTSAWQNPAAEPGK 495

Query: 123 PDWELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHVNAEQF-GGSSSSVAIAL 182
            DWELALVET S+L KQ   +GG FD LLLNGMYDQ  VR+HV+  Q  GGS+SSVA+ L
Sbjct: 496 ADWELALVETTSNLEKQTAALGGGFDNLLLNGMYDQGMVRQHVSTSQLTGGSASSVALPL 555

Query: 183 QGQGNAAAQVLALPAPDGTVEPIGYRDPFAASLTVPPPSYVQIAEMKKKQQLLTQEQLLW 242
            G+ N   QVLALPAPDGTVE +  +DPFAASLT+PPPSYVQ+AEM+KKQ LL+QEQ LW
Sbjct: 556 PGKTN--NQVLALPAPDGTVEKVN-QDPFAASLTIPPPSYVQMAEMEKKQYLLSQEQQLW 615

Query: 243 KQYGRDGMQGQVGLGKLGGG--SAYVGLNPQQIM-PHGSAYYY 278
           +QY RDGM+GQ  L K+  G   AY G+ P   M P  + YYY
Sbjct: 616 QQYQRDGMRGQASLAKMNTGPVPAY-GMPPVNGMGPPPTGYYY 649

[Close]

BLAST of Lsi06G009890.1 vs. TAIR 10
Match: AT4G32285.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 261.5 bits (667), Expect = 7.5e-70
Identity = 158/283 (55.83%), Postives = 188/283 (66.43%), Query Frame = 0


Query: 3   RRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKALPPLENCA----PVSQSVLRIQSLKE 62
           R  +  S  R E E      P  E   DMNEIKALPP EN      P  +   +   + +
Sbjct: 356 RAKRAKSPERKEIEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPAPEPKPQQPQVTD 415

Query: 63  DLVNLREDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQMPAAEFGK 122
           DLVNLRED VS D+QGNK ALALF+G P    NG WE FSS++   V+SAWQ PAAE GK
Sbjct: 416 DLVNLREDDVSGDDQGNKFALALFAGPPAN--NGKWEAFSSDN--NVTSAWQNPAAELGK 475

Query: 123 PDWELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHVN-AEQFGGSSSSVAIAL 182
            DWELALVETAS+L  QK  MGG  DPLLLNGMYDQ  VR+HV+ +E  GGSSSSVA+ L
Sbjct: 476 ADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELTGGSSSSVALPL 535

Query: 183 QGQGNAAAQVLALPAPDGTVEPIGYRDPFAASLTVPPPSYVQIAEMKKKQQLLTQEQLLW 242
            G+ N  + +LALPAPDGTV+ +  +DPFAASLT+PPPSYVQ+AEM KKQ LLTQEQ LW
Sbjct: 536 PGKVN--SHILALPAPDGTVQKVN-QDPFAASLTIPPPSYVQMAEMDKKQYLLTQEQQLW 595

Query: 243 KQYGRDGMQGQVGLGKLGGGSAYV--GLNPQQIM-PHGSAYYY 278
           +QY ++GM+GQ  L K+      +  G+ P   M P    YYY
Sbjct: 596 QQYQQEGMRGQASLAKMNTAQTAMPYGMPPVNGMGPSPMGYYY 631

[Close]

BLAST of Lsi06G009890.1 vs. TAIR 10
Match: AT4G32285.2 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 261.5 bits (667), Expect = 7.5e-70
Identity = 158/283 (55.83%), Postives = 188/283 (66.43%), Query Frame = 0


Query: 3   RRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKALPPLENCA----PVSQSVLRIQSLKE 62
           R  +  S  R E E      P  E   DMNEIKALPP EN      P  +   +   + +
Sbjct: 356 RAKRAKSPERKEIEAPPAPAPPVEEPVDMNEIKALPPPENHTPPPPPAPEPKPQQPQVTD 415

Query: 63  DLVNLREDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQMPAAEFGK 122
           DLVNLRED VS D+QGNK ALALF+G P    NG WE FSS++   V+SAWQ PAAE GK
Sbjct: 416 DLVNLREDDVSGDDQGNKFALALFAGPPAN--NGKWEAFSSDN--NVTSAWQNPAAELGK 475

Query: 123 PDWELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHVN-AEQFGGSSSSVAIAL 182
            DWELALVETAS+L  QK  MGG  DPLLLNGMYDQ  VR+HV+ +E  GGSSSSVA+ L
Sbjct: 476 ADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSELTGGSSSSVALPL 535

Query: 183 QGQGNAAAQVLALPAPDGTVEPIGYRDPFAASLTVPPPSYVQIAEMKKKQQLLTQEQLLW 242
            G+ N  + +LALPAPDGTV+ +  +DPFAASLT+PPPSYVQ+AEM KKQ LLTQEQ LW
Sbjct: 536 PGKVN--SHILALPAPDGTVQKVN-QDPFAASLTIPPPSYVQMAEMDKKQYLLTQEQQLW 595

Query: 243 KQYGRDGMQGQVGLGKLGGGSAYV--GLNPQQIM-PHGSAYYY 278
           +QY ++GM+GQ  L K+      +  G+ P   M P    YYY
Sbjct: 596 QQYQQEGMRGQASLAKMNTAQTAMPYGMPPVNGMGPSPMGYYY 631

[Close]

BLAST of Lsi06G009890.1 vs. TAIR 10
Match: AT1G03050.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 146.0 bits (367), Expect = 4.6e-35
Identity = 108/268 (40.30%), Postives = 145/268 (54.10%), Query Frame = 0


Query: 2   SRRPKDYSEMRTEEEMKLKALPVKESGYDMNEIKAL---PPLE--NCAPVSQS-----VL 61
           S   +D  E RTEE        V E   DMN IKAL   PP E  +  P  ++     + 
Sbjct: 339 SEADEDDDEARTEE--------VNEEQEDMNAIKALPEPPPKEEDDVKPEEEAKEEVIIE 398

Query: 62  RIQSLKEDLVNL-REDGVSADEQGNKLALALFSGTPITNPNGSWETFSSNHEPEVSSAWQ 121
           + Q    DL++L   +G  A + G+ LALALF G P  + +GS          E    W+
Sbjct: 399 KKQEEMGDLLDLGNTNGGEAGQAGDSLALALFDG-PYASGSGS----------ESGPGWE 458

Query: 122 MPAAEFGKPDWELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHV-NAEQFGGS 181
             A +    DWE ALV+TA++LS QK ++GG FD LLLNGMY    V   V  +  +G S
Sbjct: 459 --AFKDDSADWETALVQTATNLSGQKSELGGGFDMLLLNGMYQHGAVNAAVKTSTAYGAS 518

Query: 182 SSSVAIALQGQGNAAAQVLALPAPD------GTVEPIGYRDPFAASLTVPPPSYVQIAEM 241
            S+ ++A    G  AA +LALPAP       G +      DPFAASL V PP+YVQ+ +M
Sbjct: 519 GSASSMAFGSAGRPAATMLALPAPSTANGNAGNINSPVPMDPFAASLEVAPPAYVQMNDM 578

Query: 242 KKKQQLLTQEQLLWKQYGRDGMQGQVGL 252
           +KKQ++L +EQ++W QY RDG QG + L
Sbjct: 579 EKKQRMLMEEQMMWDQYSRDGRQGHMNL 585

[Close]

BLAST of Lsi06G009890.1 vs. TAIR 10
Match: AT4G02650.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 144.1 bits (362), Expect = 1.8e-34
Identity = 102/261 (39.08%), Postives = 139/261 (53.26%), Query Frame = 0


Query: 15  EEMKLKALPVKESGYDMNEIKALP------------PLENCAPVSQSVLRIQSLKEDLVN 74
           EE + K   ++E+  D+N IKALP             +E    V + V R Q  + DL++
Sbjct: 349 EEEESKTEYIQENQEDLNSIKALPAPEQKEEEEEEEKMETKKDVEEVVSR-QDQEGDLLD 408

Query: 75  LRED-GVSADEQGNKLALALFSGTPITN--PNGSWETFSSNHEPEVSSAWQMPAAEFGKP 134
           L ++ GV+A   G+ LALALF G   T       WE F+ N                   
Sbjct: 409 LTDEAGVTAGTVGDSLALALFDGVVGTESASGPGWEAFNDN-----------------SA 468

Query: 135 DWELALVETASHLSKQKPDMGGCFDPLLLNGMYDQATVRRHV-NAEQFGGSSSSVAIALQ 194
           DWE  LV +A+ LS QK ++GG FD LLL+GMY    V   V  +  +G S S+ ++A  
Sbjct: 469 DWETDLVRSATRLSGQKSELGGGFDTLLLDGMYQYGAVNAAVKTSTAYGSSGSASSVAFG 528

Query: 195 GQGNAAAQVLALPAPDGTVEPIGYR------DPFAASLTVPPPSYVQIAEMKKKQQLLTQ 254
             G+ AA +LALPAP  T    G R      DPFAASL V PP+YVQ+ +M+KKQ+LL +
Sbjct: 529 SAGSPAASMLALPAPPPTAN--GNRNSPVMVDPFAASLEVAPPAYVQMNDMEKKQRLLME 588

The following BLAST results are available for this feature:

BLAST of Lsi06G009890.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LF20	4.8e-69	58.30	Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q8S9J8	1.1e-68	55.83	Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9SA65	6.5e-34	40.30	Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q8GX47	2.5e-33	39.08	Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9LVD8	2.6e-06	26.12	Putative clathrin assembly protein At5g57200 OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of Lsi06G009890.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C4X9	3.5e-115	83.46	Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B992	3.5e-115	83.46	putative clathrin assembly protein At2g25430 OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A6J1F0M3	1.3e-109	76.79	probable clathrin assembly protein At4g32285 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1I3H3	2.4e-108	75.71	probable clathrin assembly protein At4g32285 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1CV16	2.7e-107	75.09	probable clathrin assembly protein At4g32285 OS=Momordica charantia OX=3673 GN=L...	[more]

BLAST of Lsi06G009890.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879765.1	2.2e-140	91.79	putative clathrin assembly protein At2g25430 [Benincasa hispida]	[more]
XP_008443967.1	7.2e-115	83.46	PREDICTED: putative clathrin assembly protein At2g25430 [Cucumis melo] >TYK06390...	[more]
KAG6587890.1	5.4e-110	77.14	putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_023529315.1	2.0e-109	77.14	putative clathrin assembly protein At2g25430 [Cucurbita pepo subsp. pepo]	[more]
XP_022934011.1	2.7e-109	76.79	probable clathrin assembly protein At4g32285 [Cucurbita moschata]	[more]

BLAST of Lsi06G009890.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G25430.1	3.4e-70	58.30	epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly p...	[more]
AT4G32285.1	7.5e-70	55.83	ENTH/ANTH/VHS superfamily protein	[more]
AT4G32285.2	7.5e-70	55.83	ENTH/ANTH/VHS superfamily protein	[more]
AT1G03050.1	4.6e-35	40.30	ENTH/ANTH/VHS superfamily protein	[more]
AT4G02650.1	1.8e-34	39.08	ENTH/ANTH/VHS superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 46..73
None	No IPR available	PANTHER	PTHR22951	CLATHRIN ASSEMBLY PROTEIN	coord: 13..262
None	No IPR available	PANTHER	PTHR22951:SF62	ASSEMBLY PLANT-LIKE PROTEIN, PUTATIVE-RELATED	coord: 13..262

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi06G009890	Lsi06G009890	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi06G009890.1.exon.1	Lsi06G009890.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi06G009890.1.CDS.1	Lsi06G009890.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi06G009890.1	Lsi06G009890.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048268	clathrin coat assembly
biological_process	GO:0072583	clathrin-dependent endocytosis
biological_process	GO:0006900	vesicle budding from membrane
cellular_component	GO:0005905	clathrin-coated pit
cellular_component	GO:0030136	clathrin-coated vesicle
cellular_component	GO:0005794	Golgi apparatus
molecular_function	GO:0005545	1-phosphatidylinositol binding
molecular_function	GO:0032050	clathrin heavy chain binding
molecular_function	GO:0005546	phosphatidylinositol-4,5-bisphosphate binding
molecular_function	GO:0000149	SNARE binding