Lsi03G013200.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G013200.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	t-SNARE coiled-coil homology domain-containing protein
Location	chr03: 24003646 .. 24004574 (-)
Sequence length	336
RNA-Seq Expression	Lsi03G013200.1
Synteny	Lsi03G013200.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACTTGATAATGTTTTGAAGGATTCCTTTGAGATTCCTCGGGGTCAACTTTCTAGAGGAGGAGACATTGAGCTAGGAACAAATGTTCCTACTAGTGGAGGGGATGGCTTGGAGGATTTCTTTAAAAAGGTATTTAAAGAATTTATTTTGCCTCCTATTGTCAAGCAAGTAGTAAATTTGATGTTGCTATATAAGAGTGTTTTCAATAACTCAGCTTGAGTGGATACGTTGTACTTGTGTTCTTTCATCTTATCCAGGTCCAAGAGATTGAAAAACAGAATGAGAAGCTGGATAGGCTATTGAGAAAGCTCCAGGTATGTTTATTATTCTACCTTTTGGTGATATTTGTTGTTATTTTGAATGCAACTCCCTGTTGAAGATATTCAAGTTTTATTATTTTTTTCTTTTTTTGCGTGACTTTGTGGGGACTCCAGTGTGAAGATGAAGATTTGATGATTCTAGGGTTTTGATTATTTATTTATTTATTCTTTTAGTTTTGGGCTCAGGCAACGAACTTTTTAAATAGGTTGCGTACTTTTGGATTGGGAAATATCTTTTCTTTTTAAAAAAGTGTGTCCCCATCTTGTATCTGGAAGGGGACACACGTATCCGATATTTTAAAAAAAAAGATATGCCAAATCGCATATCAGATACGTATATGAAAGTATCGATATTTGATCTGCCTTACACGAAGTATTTGTGCTTCGTAGGAAGCTATTACTTGGAGGTTAATGTCTGCAACATAAGAGAAACAACAATATGTCACATTCTTGGATAAGGACAATGAAGATTTTGAATTATCTTGCCAAAGGGATCAGGAGACAAATATGGAAAATGGTTCCAAAATCTCTTTAAAGCCCATAGACGTAGAGCAAGAGAGGAAAGGCAAGAAACCCATACAAGAAAATGAACCCCAGGAAAGGTGA

mRNA sequence

ATGGAACTTGATAATGTTTTGAAGGATTCCTTTGAGATTCCTCGGGGTCAACTTTCTAGAGGAGGAGACATTGAGCTAGGAACAAATGTTCCTACTAGTGGAGGGGATGGCTTGGAGGATTTCTTTAAAAAGGTCCAAGAGATTGAAAAACAGAATGAGAAGCTGGATAGGCTATTGAGAAAGCTCCAGGACAATGAAGATTTTGAATTATCTTGCCAAAGGGATCAGGAGACAAATATGGAAAATGGTTCCAAAATCTCTTTAAAGCCCATAGACGTAGAGCAAGAGAGGAAAGGCAAGAAACCCATACAAGAAAATGAACCCCAGGAAAGGTGA

Coding sequence (CDS)

ATGGAACTTGATAATGTTTTGAAGGATTCCTTTGAGATTCCTCGGGGTCAACTTTCTAGAGGAGGAGACATTGAGCTAGGAACAAATGTTCCTACTAGTGGAGGGGATGGCTTGGAGGATTTCTTTAAAAAGGTCCAAGAGATTGAAAAACAGAATGAGAAGCTGGATAGGCTATTGAGAAAGCTCCAGGACAATGAAGATTTTGAATTATCTTGCCAAAGGGATCAGGAGACAAATATGGAAAATGGTTCCAAAATCTCTTTAAAGCCCATAGACGTAGAGCAAGAGAGGAAAGGCAAGAAACCCATACAAGAAAATGAACCCCAGGAAAGGTGA

Protein sequence

MELDNVLKDSFEIPRGQLSRGGDIELGTNVPTSGGDGLEDFFKKVQEIEKQNEKLDRLLRKLQDNEDFELSCQRDQETNMENGSKISLKPIDVEQERKGKKPIQENEPQER

Homology

[Close]

BLAST of Lsi03G013200.1 vs. ExPASy Swiss-Prot
Match: Q8VZU2 (Syntaxin-132 OS=Arabidopsis thaliana OX=3702 GN=SYP132 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 2.1e-12
Identity = 40/66 (60.61%), Postives = 52/66 (78.79%), Query Frame = 0


Query: 3  LDNVLKDSFEIPRGQLSRGGDIELGTNVPTSGGD-GLEDFFKKVQEIEKQNEKLDRLLRK 62
          ++++LK SFE+PRGQ SR GD+ELG      GGD GLEDFFKKVQ I+KQ +KLD+LL+K
Sbjct: 1  MNDLLKGSFELPRGQSSREGDVELGEQ---QGGDQGLEDFFKKVQVIDKQYDKLDKLLKK 60

Query: 63 LQDNED 68
          LQ + +
Sbjct: 61 LQASHE 63

[Close]

BLAST of Lsi03G013200.1 vs. ExPASy Swiss-Prot
Match: Q9SRV7 (Putative syntaxin-131 OS=Arabidopsis thaliana OX=3702 GN=SYP131 PE=3 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 4.6e-07
Identity = 33/61 (54.10%), Postives = 40/61 (65.57%), Query Frame = 0


Query: 3  LDNVLKDSFEIPRGQLSRGGDIELGTNVPTSGGDGLEDFFKKVQEIEKQNEKLDRLLRKL 62
          ++++LK S E  R + +R  DIE G     SG  GL  FFKKVQEIEKQ EKLD+ L KL
Sbjct: 1  MNDLLKGSLEFSRDRSNR-SDIESGHGPGNSGDLGLSGFFKKVQEIEKQYEKLDKHLNKL 60

Query: 63 Q 64
          Q
Sbjct: 61 Q 60

[Close]

BLAST of Lsi03G013200.1 vs. ExPASy TrEMBL
Match: A0A6J1IZY0 (syntaxin-132-like OS=Cucurbita maxima OX=3661 GN=LOC111479972 PE=3 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 7.1e-19
Identity = 52/66 (78.79%), Postives = 60/66 (90.91%), Query Frame = 0


Query: 3  LDNVLKDSFEIPRGQLSRGGDIELGTNVPTSGGD-GLEDFFKKVQEIEKQNEKLDRLLRK 62
          ++++L DSFEIPRGQ SRGGDIELGTN PTS GD GL+DFFKKVQ+IEKQNEKLDRLLRK
Sbjct: 1  MNDLLSDSFEIPRGQPSRGGDIELGTNAPTSAGDLGLDDFFKKVQDIEKQNEKLDRLLRK 60

Query: 63 LQDNED 68
          LQD+ +
Sbjct: 61 LQDSHE 66

[Close]

BLAST of Lsi03G013200.1 vs. ExPASy TrEMBL
Match: A0A6J1ERW7 (syntaxin-132-like OS=Cucurbita moschata OX=3662 GN=LOC111436996 PE=3 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 7.1e-19
Identity = 52/66 (78.79%), Postives = 60/66 (90.91%), Query Frame = 0


Query: 3  LDNVLKDSFEIPRGQLSRGGDIELGTNVPTSGGD-GLEDFFKKVQEIEKQNEKLDRLLRK 62
          ++++L DSFEIPRGQ SRGGDIELGTN PTS GD GL+DFFKKVQ+IEKQNEKLDRLLRK
Sbjct: 1  MNDLLSDSFEIPRGQPSRGGDIELGTNAPTSAGDLGLDDFFKKVQDIEKQNEKLDRLLRK 60

Query: 63 LQDNED 68
          LQD+ +
Sbjct: 61 LQDSHE 66

[Close]

BLAST of Lsi03G013200.1 vs. ExPASy TrEMBL
Match: A0A1S3CPB6 (syntaxin-132 OS=Cucumis melo OX=3656 GN=LOC103502715 PE=3 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 7.3e-16
Identity = 48/65 (73.85%), Postives = 55/65 (84.62%), Query Frame = 0


Query: 3  LDNVLKDSFEIPRGQLSRGGDIELGTNVPTSGGDGLEDFFKKVQEIEKQNEKLDRLLRKL 62
          ++++L DSFEIPRGQ SRGGDIELGTN P  G  G+ DFFKKVQEIEKQNEKLD LLRKL
Sbjct: 1  MNDLLSDSFEIPRGQPSRGGDIELGTNAP--GDQGMGDFFKKVQEIEKQNEKLDTLLRKL 60

Query: 63 QDNED 68
          QD+ +
Sbjct: 61 QDSHE 63

[Close]

BLAST of Lsi03G013200.1 vs. ExPASy TrEMBL
Match: A0A2P5BK18 (t-SNARE OS=Parasponia andersonii OX=3476 GN=PanSYP131 PE=3 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 9.6e-16
Identity = 47/63 (74.60%), Postives = 54/63 (85.71%), Query Frame = 0


Query: 3  LDNVLKDSFEIPRGQLSRGGDIELGTNVPTSGGD-GLEDFFKKVQEIEKQNEKLDRLLRK 62
          ++++L DSFEIPRGQ SR GD+ELGT  P + GD GLE FFKKVQEIEKQNEKLD+LLRK
Sbjct: 1  MNDLLSDSFEIPRGQTSRDGDVELGTQAPMNPGDQGLETFFKKVQEIEKQNEKLDKLLRK 60

Query: 63 LQD 65
          LQD
Sbjct: 61 LQD 63

[Close]

BLAST of Lsi03G013200.1 vs. ExPASy TrEMBL
Match: A0A7J6FCY3 (t-SNARE coiled-coil homology domain-containing protein OS=Cannabis sativa OX=3483 GN=F8388_018675 PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.2e-15
Identity = 48/63 (76.19%), Postives = 54/63 (85.71%), Query Frame = 0


Query: 3  LDNVLKDSFEIPRGQLSRGGDIELGTNVPTSGGD-GLEDFFKKVQEIEKQNEKLDRLLRK 62
          ++++L DSFEIPRGQ SR GDIELGT  P + GD GLE FFKKVQEIEKQNEKLD+LLRK
Sbjct: 1  MNDLLTDSFEIPRGQPSRDGDIELGTQAPMNTGDQGLESFFKKVQEIEKQNEKLDKLLRK 60

Query: 63 LQD 65
          LQD
Sbjct: 61 LQD 63

[Close]

BLAST of Lsi03G013200.1 vs. NCBI nr
Match: XP_038905131.1 (syntaxin-132-like [Benincasa hispida])

HSP 1 Score: 105.9 bits (263), Expect = 2.3e-19
Identity = 54/66 (81.82%), Postives = 61/66 (92.42%), Query Frame = 0


Query: 3  LDNVLKDSFEIPRG-QLSRGGDIELGTNVPTSGGDGLEDFFKKVQEIEKQNEKLDRLLRK 62
          ++++L DSFEIPRG Q SRGGDIELG+NVP SGGDGLEDFFKKVQEIEKQNEKLDRLLRK
Sbjct: 1  MNDLLSDSFEIPRGRQPSRGGDIELGSNVPASGGDGLEDFFKKVQEIEKQNEKLDRLLRK 60

Query: 63 LQDNED 68
          LQD+ +
Sbjct: 61 LQDSHE 66

[Close]

BLAST of Lsi03G013200.1 vs. NCBI nr
Match: XP_022930594.1 (syntaxin-132-like [Cucurbita moschata])

HSP 1 Score: 103.2 bits (256), Expect = 1.5e-18
Identity = 52/66 (78.79%), Postives = 60/66 (90.91%), Query Frame = 0


Query: 3  LDNVLKDSFEIPRGQLSRGGDIELGTNVPTSGGD-GLEDFFKKVQEIEKQNEKLDRLLRK 62
          ++++L DSFEIPRGQ SRGGDIELGTN PTS GD GL+DFFKKVQ+IEKQNEKLDRLLRK
Sbjct: 1  MNDLLSDSFEIPRGQPSRGGDIELGTNAPTSAGDLGLDDFFKKVQDIEKQNEKLDRLLRK 60

Query: 63 LQDNED 68
          LQD+ +
Sbjct: 61 LQDSHE 66

[Close]

BLAST of Lsi03G013200.1 vs. NCBI nr
Match: KAG7018148.1 (putative syntaxin [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 103.2 bits (256), Expect = 1.5e-18
Identity = 52/66 (78.79%), Postives = 60/66 (90.91%), Query Frame = 0


Query: 3  LDNVLKDSFEIPRGQLSRGGDIELGTNVPTSGGD-GLEDFFKKVQEIEKQNEKLDRLLRK 62
          ++++L DSFEIPRGQ SRGGDIELGTN PTS GD GL+DFFKKVQ+IEKQNEKLDRLLRK
Sbjct: 1  MNDLLSDSFEIPRGQPSRGGDIELGTNAPTSAGDLGLDDFFKKVQDIEKQNEKLDRLLRK 60

Query: 63 LQDNED 68
          LQD+ +
Sbjct: 61 LQDSHE 66

[Close]

BLAST of Lsi03G013200.1 vs. NCBI nr
Match: XP_022980674.1 (syntaxin-132-like [Cucurbita maxima] >XP_023527817.1 syntaxin-132-like [Cucurbita pepo subsp. pepo] >KAG6581655.1 putative syntaxin-131, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 103.2 bits (256), Expect = 1.5e-18
Identity = 52/66 (78.79%), Postives = 60/66 (90.91%), Query Frame = 0


Query: 3  LDNVLKDSFEIPRGQLSRGGDIELGTNVPTSGGD-GLEDFFKKVQEIEKQNEKLDRLLRK 62
          ++++L DSFEIPRGQ SRGGDIELGTN PTS GD GL+DFFKKVQ+IEKQNEKLDRLLRK
Sbjct: 1  MNDLLSDSFEIPRGQPSRGGDIELGTNAPTSAGDLGLDDFFKKVQDIEKQNEKLDRLLRK 60

Query: 63 LQDNED 68
          LQD+ +
Sbjct: 61 LQDSHE 66

[Close]

BLAST of Lsi03G013200.1 vs. NCBI nr
Match: XP_038695044.1 (syntaxin-132-like [Tripterygium wilfordii])

HSP 1 Score: 98.6 bits (244), Expect = 3.6e-17
Identity = 49/63 (77.78%), Postives = 57/63 (90.48%), Query Frame = 0


Query: 3  LDNVLKDSFEIPRGQLSRGGDIELGTNVPTSGGD-GLEDFFKKVQEIEKQNEKLDRLLRK 62
          ++++L DSFEIPRGQ SRGGDIE+GTN P + GD GLEDFFKKVQEIEKQNEKLD+LL+K
Sbjct: 1  MNDLLADSFEIPRGQPSRGGDIEMGTNAPMNSGDLGLEDFFKKVQEIEKQNEKLDKLLKK 60

Query: 63 LQD 65
          LQD
Sbjct: 61 LQD 63

[Close]

BLAST of Lsi03G013200.1 vs. TAIR 10
Match: AT5G08080.3 (syntaxin of plants 132 )

HSP 1 Score: 74.7 bits (182), Expect = 5.2e-14
Identity = 44/79 (55.70%), Postives = 58/79 (73.42%), Query Frame = 0


Query: 3  LDNVLKDSFEIPRGQLSRGGDIELGTNVPTSGGD-GLEDFFKKVQEIEKQNEKLDRLLRK 62
          ++++LK SFE+PRGQ SR GD+ELG      GGD GLEDFFKKVQ I+KQ +KLD+LL+K
Sbjct: 1  MNDLLKGSFELPRGQSSREGDVELGEQ---QGGDQGLEDFFKKVQVIDKQYDKLDKLLKK 60

Query: 63 LQ--DNEDFELSCQRDQET 79
          LQ  D+   +L C   +E+
Sbjct: 61 LQIYDSVASQLPCASHEES 76

[Close]

BLAST of Lsi03G013200.1 vs. TAIR 10
Match: AT5G08080.1 (syntaxin of plants 132 )

HSP 1 Score: 73.2 bits (178), Expect = 1.5e-13
Identity = 40/66 (60.61%), Postives = 52/66 (78.79%), Query Frame = 0


Query: 3  LDNVLKDSFEIPRGQLSRGGDIELGTNVPTSGGD-GLEDFFKKVQEIEKQNEKLDRLLRK 62
          ++++LK SFE+PRGQ SR GD+ELG      GGD GLEDFFKKVQ I+KQ +KLD+LL+K
Sbjct: 1  MNDLLKGSFELPRGQSSREGDVELGEQ---QGGDQGLEDFFKKVQVIDKQYDKLDKLLKK 60

Query: 63 LQDNED 68
          LQ + +
Sbjct: 61 LQASHE 63

[Close]

BLAST of Lsi03G013200.1 vs. TAIR 10
Match: AT5G08080.2 (syntaxin of plants 132 )

HSP 1 Score: 73.2 bits (178), Expect = 1.5e-13
Identity = 40/66 (60.61%), Postives = 52/66 (78.79%), Query Frame = 0


Query: 3  LDNVLKDSFEIPRGQLSRGGDIELGTNVPTSGGD-GLEDFFKKVQEIEKQNEKLDRLLRK 62
          ++++LK SFE+PRGQ SR GD+ELG      GGD GLEDFFKKVQ I+KQ +KLD+LL+K
Sbjct: 1  MNDLLKGSFELPRGQSSREGDVELGEQ---QGGDQGLEDFFKKVQVIDKQYDKLDKLLKK 60

Query: 63 LQDNED 68
          LQ + +
Sbjct: 61 LQASHE 63

[Close]

BLAST of Lsi03G013200.1 vs. TAIR 10
Match: AT3G03800.1 (syntaxin of plants 131 )

HSP 1 Score: 55.5 bits (132), Expect = 3.3e-08
Identity = 33/61 (54.10%), Postives = 40/61 (65.57%), Query Frame = 0


Query: 3  LDNVLKDSFEIPRGQLSRGGDIELGTNVPTSGGDGLEDFFKKVQEIEKQNEKLDRLLRKL 62
          ++++LK S E  R + +R  DIE G     SG  GL  FFKKVQEIEKQ EKLD+ L KL
Sbjct: 1  MNDLLKGSLEFSRDRSNR-SDIESGHGPGNSGDLGLSGFFKKVQEIEKQYEKLDKHLNKL 60

Query: 63 Q 64
          Q
Sbjct: 61 Q 60

The following BLAST results are available for this feature:

BLAST of Lsi03G013200.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VZU2	2.1e-12	60.61	Syntaxin-132 OS=Arabidopsis thaliana OX=3702 GN=SYP132 PE=1 SV=1	[more]
Q9SRV7	4.6e-07	54.10	Putative syntaxin-131 OS=Arabidopsis thaliana OX=3702 GN=SYP131 PE=3 SV=1	[more]

BLAST of Lsi03G013200.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IZY0	7.1e-19	78.79	syntaxin-132-like OS=Cucurbita maxima OX=3661 GN=LOC111479972 PE=3 SV=1	[more]
A0A6J1ERW7	7.1e-19	78.79	syntaxin-132-like OS=Cucurbita moschata OX=3662 GN=LOC111436996 PE=3 SV=1	[more]
A0A1S3CPB6	7.3e-16	73.85	syntaxin-132 OS=Cucumis melo OX=3656 GN=LOC103502715 PE=3 SV=1	[more]
A0A2P5BK18	9.6e-16	74.60	t-SNARE OS=Parasponia andersonii OX=3476 GN=PanSYP131 PE=3 SV=1	[more]
A0A7J6FCY3	1.2e-15	76.19	t-SNARE coiled-coil homology domain-containing protein OS=Cannabis sativa OX=348...	[more]

BLAST of Lsi03G013200.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905131.1	2.3e-19	81.82	syntaxin-132-like [Benincasa hispida]	[more]
XP_022930594.1	1.5e-18	78.79	syntaxin-132-like [Cucurbita moschata]	[more]
KAG7018148.1	1.5e-18	78.79	putative syntaxin [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022980674.1	1.5e-18	78.79	syntaxin-132-like [Cucurbita maxima] >XP_023527817.1 syntaxin-132-like [Cucurbit...	[more]
XP_038695044.1	3.6e-17	77.78	syntaxin-132-like [Tripterygium wilfordii]	[more]

BLAST of Lsi03G013200.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08080.3	5.2e-14	55.70	syntaxin of plants 132	[more]
AT5G08080.1	1.5e-13	60.61	syntaxin of plants 132	[more]
AT5G08080.2	1.5e-13	60.61	syntaxin of plants 132	[more]
AT3G03800.1	3.3e-08	54.10	syntaxin of plants 131	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 38..68
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 71..111
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 17..36
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 90..111
None	No IPR available	PANTHER	PTHR19957:SF329	SUBFAMILY NOT NAMED	coord: 9..67
None	No IPR available	PANTHER	PTHR19957	SYNTAXIN	coord: 9..67

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi03G013200	Lsi03G013200	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi03G013200.1.exon.3	Lsi03G013200.1.exon.3	exon
Lsi03G013200.1.exon.2	Lsi03G013200.1.exon.2	exon
Lsi03G013200.1.exon.1	Lsi03G013200.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi03G013200.1.CDS.3	Lsi03G013200.1.CDS.3	CDS
Lsi03G013200.1.CDS.2	Lsi03G013200.1.CDS.2	CDS
Lsi03G013200.1.CDS.1	Lsi03G013200.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi03G013200.1	Lsi03G013200.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006887	exocytosis
biological_process	GO:0006886	intracellular protein transport
biological_process	GO:0048278	vesicle docking
biological_process	GO:0006906	vesicle fusion
cellular_component	GO:0009504	cell plate
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0031201	SNARE complex
cellular_component	GO:0005802	trans-Golgi network
molecular_function	GO:0005484	SNAP receptor activity
molecular_function	GO:0000149	SNARE binding