Lsi03G012970.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G012970.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	chr03: 23782101 .. 23782982 (-)
Sequence length	618
RNA-Seq Expression	Lsi03G012970.1
Synteny	Lsi03G012970.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCAGAATCAGGCCCTGCAGTGCCTGCTTTGCCTCTGCAGGATCGAGTGGCCATTGTGACTGGTGCCTCTCGGGGCATAGGCCGAGCCATTGCCCTTCACCTCGCTGTTCTTGGCGCCCGACTTGTCATCAACTATGTCACTAGTTCAGCTGAAGCCGATCGAGTTGCTGCCGATATCAACTCATCCTCGGCCGCAGGGAGCAGCCAACGAGCCATTGCTTGGCGGGCGGATGTGTCTGATCCAGAGCAAGTGAAGTCTCTGTTTGACGCAGCCGAGCAGGCATTTGGCTCCCAGGTCCATATCCTGGTTAACTCTGCTGGAATTTCAGATCCTACTTACCCTTATATTGCTGATACTTCACTGGAGATTTTCGACCAAATTTTCAGGTGCTTCACTCTTCTCCCCTAAGATTTCTTTGGAAAAAGTTTAATTTTCACTTCCCATTTCATTTGGCTGAAGCTTAAGCATAACTCAATAATTTACATAAAGACTCAACAAATGGTTATGAATATTAATTAGAAGAGAAAACAACTTAATTGAAGTTGGAATATAGAACTTCTTCTAATATCATGTTAAACGACACTGATGAGTAAAATAGTTGAGTTTGTTCTTCAGTGTGAACACGAGGGGATGTTTCCTGTGCTGCAAAGAAGCAGCAAACCGAGTGAAACGCGGCGGTGGAGGGCGAATAATACTGATATCGTCGACAGCGGTGGCAGCAGCAACAGCAGGATTAGGAGCGTACACAGCGTCGAAGGCAGCGGTGGAGGCAATGACGAAGGTGGCGGCGAAGGAGCTGAGTGGGAGTGGAATATCGGTGAACTGCATAGCGCCAGGGGCGACGGCGACAGAGATGTTTTACAGAGGAATGAATGA

mRNA sequence

ATGGCTTCAGAATCAGGCCCTGCAGTGCCTGCTTTGCCTCTGCAGGATCGAGTGGCCATTGTGACTGGTGCCTCTCGGGGCATAGGCCGAGCCATTGCCCTTCACCTCGCTGTTCTTGGCGCCCGACTTGTCATCAACTATGTCACTAGTTCAGCTGAAGCCGATCGAGTTGCTGCCGATATCAACTCATCCTCGGCCGCAGGGAGCAGCCAACGAGCCATTGCTTGGCGGGCGGATGTGTCTGATCCAGAGCAAGTGAAGTCTCTGTTTGACGCAGCCGAGCAGGCATTTGGCTCCCAGGTCCATATCCTGGTTAACTCTGCTGGAATTTCAGATCCTACTTACCCTTATATTGCTGATACTTCACTGGAGATTTTCGACCAAATTTTCAGAAGCAGCAAACCGAGTGAAACGCGGCGGTGGAGGGCGAATAATACTGATATCGTCGACAGCGGTGGCAGCAGCAACAGCAGGATTAGGAGCGTACACAGCGTCGAAGGCAGCGGTGGAGGCAATGACGAAGGTGGCGGCGAAGGAGCTGAGTGGGAGTGGAATATCGGTGAACTGCATAGCGCCAGGGGCGACGGCGACAGAGATGTTTTACAGAGGAATGAATGA

Coding sequence (CDS)

ATGGCTTCAGAATCAGGCCCTGCAGTGCCTGCTTTGCCTCTGCAGGATCGAGTGGCCATTGTGACTGGTGCCTCTCGGGGCATAGGCCGAGCCATTGCCCTTCACCTCGCTGTTCTTGGCGCCCGACTTGTCATCAACTATGTCACTAGTTCAGCTGAAGCCGATCGAGTTGCTGCCGATATCAACTCATCCTCGGCCGCAGGGAGCAGCCAACGAGCCATTGCTTGGCGGGCGGATGTGTCTGATCCAGAGCAAGTGAAGTCTCTGTTTGACGCAGCCGAGCAGGCATTTGGCTCCCAGGTCCATATCCTGGTTAACTCTGCTGGAATTTCAGATCCTACTTACCCTTATATTGCTGATACTTCACTGGAGATTTTCGACCAAATTTTCAGAAGCAGCAAACCGAGTGAAACGCGGCGGTGGAGGGCGAATAATACTGATATCGTCGACAGCGGTGGCAGCAGCAACAGCAGGATTAGGAGCGTACACAGCGTCGAAGGCAGCGGTGGAGGCAATGACGAAGGTGGCGGCGAAGGAGCTGAGTGGGAGTGGAATATCGGTGAACTGCATAGCGCCAGGGGCGACGGCGACAGAGATGTTTTACAGAGGAATGAATGA

Protein sequence

MASESGPAVPALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAADINSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIADTSLEIFDQIFRSSKPSETRRWRANNTDIVDSGGSSNSRIRSVHSVEGSGGGNDEGGGEGAEWEWNIGELHSARGDGDRDVLQRNE

Homology

[Close]

BLAST of Lsi03G012970.1 vs. ExPASy Swiss-Prot
Match: Q9SQR4 (NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g03980 PE=3 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 2.8e-34
Identity = 82/134 (61.19%), Postives = 100/134 (74.63%), Query Frame = 0


Query: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAAD 60
           M++ S  + P LPL  RVAIVTG+SRGIGRAIA+HLA LGAR+VINY + +A+A+RVA++
Sbjct: 1   MSTHSSISQPPLPLAGRVAIVTGSSRGIGRAIAIHLAELGARIVINYTSKAADAERVASE 60

Query: 61  INS----SSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYP 120
           IN         G   RAI  +A+VS+P QVKS+FDAAE AF + VHILVNSAGI DP YP
Sbjct: 61  INDFPVREEITGKGPRAIVVQANVSEPSQVKSMFDAAESAFEAPVHILVNSAGILDPKYP 120

Query: 121 YIADTSLEIFDQIF 131
            IADTS+E FD  F
Sbjct: 121 TIADTSVEDFDHTF 134

[Close]

BLAST of Lsi03G012970.1 vs. ExPASy Swiss-Prot
Match: Q9SQR2 (NADPH-dependent aldehyde reductase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ChlADR2 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.1e-30
Identity = 77/134 (57.46%), Postives = 97/134 (72.39%), Query Frame = 0


Query: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAAD 60
           MA+ S  + P L L  RVAIVTG+SRGIGRAIA+HLA LGAR+V+NY TS  EA++VA  
Sbjct: 1   MAAASSVSSPPLCLAGRVAIVTGSSRGIGRAIAIHLAELGARVVVNYSTSPVEAEKVATA 60

Query: 61  INS-----SSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTY 120
           I +     +  AG S R I  +AD+S+P QVKSLFD AE+ F S VHILVNSA I+DP +
Sbjct: 61  ITTNCSKDAEVAGKSPRVIVVKADISEPSQVKSLFDEAERVFESPVHILVNSAAIADPNH 120

Query: 121 PYIADTSLEIFDQI 130
             I+D S+E+FD+I
Sbjct: 121 STISDMSVELFDRI 134

[Close]

BLAST of Lsi03G012970.1 vs. ExPASy Swiss-Prot
Match: Q08632 (Short-chain type dehydrogenase/reductase OS=Picea abies OX=3329 PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.0e-24
Identity = 66/121 (54.55%), Postives = 90/121 (74.38%), Query Frame = 0


Query: 12  LPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAADINSSS-AAGSS 71
           LPL  RVAIVTGASRGIGR IAL++A  GA++VI+Y ++   A+ VA+ IN+ S ++G  
Sbjct: 17  LPLGGRVAIVTGASRGIGREIALNMAEKGAKVVIHYSSNQHAAEEVASIINNKSPSSGDG 76

Query: 72  QRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIADTSLEIFDQIF 131
            RAI  +ADV++P QV  LFD AE AFG  +HI+VN+AG++D  YP +A TS E +D+IF
Sbjct: 77  VRAIVCKADVAEPSQVAQLFDTAEHAFG-PLHIVVNNAGVTDSKYPTLAQTSDEEWDRIF 136

[Close]

BLAST of Lsi03G012970.1 vs. ExPASy Swiss-Prot
Match: P0CU75 (Short chain dehydrogenase claC OS=Passalora fulva OX=5499 GN=claC PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.9e-14
Identity = 53/130 (40.77%), Postives = 78/130 (60.00%), Query Frame = 0


Query: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAAD 60
           MA  +G  +P   L  RVA+VTG+ RGIG AIA+HL  LGA +V+NY  S+ +A +V   
Sbjct: 1   MAVVNGNYIPG-RLDGRVAVVTGSGRGIGAAIAVHLGRLGANIVVNYANSALDAQKVVDQ 60

Query: 61  INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           I      G+   AIA +AD+ D  Q+  LFD A   FG  V I V+++G+   ++ ++ D
Sbjct: 61  IK-----GAGSEAIAIKADIRDVSQIMRLFDEAVAHFG-HVDIAVSNSGV--VSFGHLKD 120

Query: 121 TSLEIFDQIF 131
            + E FD++F
Sbjct: 121 VTEEEFDRVF 121

[Close]

BLAST of Lsi03G012970.1 vs. ExPASy Swiss-Prot
Match: W3XC32 (Trihydroxynaphthalene reductase PfmaI OS=Pestalotiopsis fici (strain W106-1 / CGMCC3.15140) OX=1229662 GN=Pfmaj PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.7e-13
Identity = 46/117 (39.32%), Postives = 79/117 (67.52%), Query Frame = 0


Query: 14  LQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAADINSSSAAGSSQRA 73
           L+ +VA+VTGA RGIGR +A+ L   GA++++NY  SS  A  V   +N+   +GS   A
Sbjct: 27  LEGKVALVTGAGRGIGREMAMELGRRGAKVIVNYANSSESAQEV---VNAIKKSGSD--A 86

Query: 74  IAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIADTSLEIFDQIF 131
           +A +A+VSD +Q+ SLFD A + +G ++HI+ +++G+   ++ ++ D + E FD++F
Sbjct: 87  VAIKANVSDVDQIVSLFDQAVKVWG-KLHIVCSNSGV--VSFGHVKDVTPEEFDRVF 135

[Close]

BLAST of Lsi03G012970.1 vs. ExPASy TrEMBL
Match: A0A0A0LI83 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G843800 PE=4 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 3.0e-55
Identity = 119/130 (91.54%), Postives = 125/130 (96.15%), Query Frame = 0


Query: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAAD 60
           MASESGPAVPALPLQDRVAIVTGASRGIGR IALHLA LGAR+V+NYV+SSAEAD+V AD
Sbjct: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRGIALHLAGLGARVVVNYVSSSAEADQVVAD 60

Query: 61  INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIA+
Sbjct: 61  INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAN 120

Query: 121 TSLEIFDQIF 131
           T LEIFD +F
Sbjct: 121 TPLEIFDHLF 130

[Close]

BLAST of Lsi03G012970.1 vs. ExPASy TrEMBL
Match: A0A5A7U7W3 (Photosynthetic NDH subunit of subcomplex B 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold734G00100 PE=4 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 1.1e-54
Identity = 118/130 (90.77%), Postives = 123/130 (94.62%), Query Frame = 0


Query: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAAD 60
           MASESGPAVPALPLQDRVAIVTGASRGIGR IALHLA LGAR+V+NYV SSAEAD+V A 
Sbjct: 365 MASESGPAVPALPLQDRVAIVTGASRGIGRGIALHLAGLGARVVVNYVASSAEADQVVAS 424

Query: 61  INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INS+SAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD
Sbjct: 425 INSTSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 484

Query: 121 TSLEIFDQIF 131
           T LEIFD +F
Sbjct: 485 TPLEIFDHLF 494

[Close]

BLAST of Lsi03G012970.1 vs. ExPASy TrEMBL
Match: A0A1S3CP09 (short-chain type dehydrogenase/reductase-like OS=Cucumis melo OX=3656 GN=LOC103502971 PE=4 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 1.1e-54
Identity = 118/130 (90.77%), Postives = 123/130 (94.62%), Query Frame = 0


Query: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAAD 60
           MASESGPAVPALPLQDRVAIVTGASRGIGR IALHLA LGAR+V+NYV SSAEAD+V A 
Sbjct: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRGIALHLAGLGARVVVNYVASSAEADQVVAS 60

Query: 61  INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INS+SAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD
Sbjct: 61  INSTSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120

Query: 121 TSLEIFDQIF 131
           T LEIFD +F
Sbjct: 121 TPLEIFDHLF 130

[Close]

BLAST of Lsi03G012970.1 vs. ExPASy TrEMBL
Match: A0A6J1F321 (NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111439224 PE=4 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 1.5e-51
Identity = 111/130 (85.38%), Postives = 120/130 (92.31%), Query Frame = 0


Query: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAAD 60
           MASESGPA+ ALPLQDRV IVTGASRGIGRAIA HLA LGAR+V+NYV+SSA+ADRVAA+
Sbjct: 1   MASESGPAMAALPLQDRVVIVTGASRGIGRAIAFHLAALGARIVVNYVSSSAKADRVAAE 60

Query: 61  INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INSSSA GS  RAIAWRADVS+PEQVKSLFD AEQ FG+QVHI+VN AGISDPTYPYIAD
Sbjct: 61  INSSSATGSGLRAIAWRADVSNPEQVKSLFDTAEQTFGAQVHIMVNCAGISDPTYPYIAD 120

Query: 121 TSLEIFDQIF 131
           TSLEIFDQIF
Sbjct: 121 TSLEIFDQIF 130

[Close]

BLAST of Lsi03G012970.1 vs. ExPASy TrEMBL
Match: A0A6J1I5H5 (NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111470837 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 2.9e-50
Identity = 109/130 (83.85%), Postives = 118/130 (90.77%), Query Frame = 0


Query: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAAD 60
           MASESGPA+ +LPLQDRV IVTGASRGIGRAIA HLA LGAR+V+NYV+SSA+ADRVAA+
Sbjct: 1   MASESGPAMASLPLQDRVVIVTGASRGIGRAIAFHLAALGARIVVNYVSSSAKADRVAAE 60

Query: 61  INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INSSSA  S  RAIAWRADVSDPEQVKSLFD AEQ FG+QVHI+VN AGISDPTYPYIAD
Sbjct: 61  INSSSATMSGLRAIAWRADVSDPEQVKSLFDTAEQTFGAQVHIMVNCAGISDPTYPYIAD 120

Query: 121 TSLEIFDQIF 131
           T LEIFDQIF
Sbjct: 121 TKLEIFDQIF 130

[Close]

BLAST of Lsi03G012970.1 vs. NCBI nr
Match: KGN59874.2 (hypothetical protein Csa_001420 [Cucumis sativus])

HSP 1 Score: 224.9 bits (572), Expect = 6.2e-55
Identity = 119/130 (91.54%), Postives = 125/130 (96.15%), Query Frame = 0


Query: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAAD 60
           MASESGPAVPALPLQDRVAIVTGASRGIGR IALHLA LGAR+V+NYV+SSAEAD+V AD
Sbjct: 366 MASESGPAVPALPLQDRVAIVTGASRGIGRGIALHLAGLGARVVVNYVSSSAEADQVVAD 425

Query: 61  INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIA+
Sbjct: 426 INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAN 485

Query: 121 TSLEIFDQIF 131
           T LEIFD +F
Sbjct: 486 TPLEIFDHLF 495

[Close]

BLAST of Lsi03G012970.1 vs. NCBI nr
Match: XP_004141209.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic [Cucumis sativus])

HSP 1 Score: 224.9 bits (572), Expect = 6.2e-55
Identity = 119/130 (91.54%), Postives = 125/130 (96.15%), Query Frame = 0


Query: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAAD 60
           MASESGPAVPALPLQDRVAIVTGASRGIGR IALHLA LGAR+V+NYV+SSAEAD+V AD
Sbjct: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRGIALHLAGLGARVVVNYVSSSAEADQVVAD 60

Query: 61  INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIA+
Sbjct: 61  INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAN 120

Query: 121 TSLEIFDQIF 131
           T LEIFD +F
Sbjct: 121 TPLEIFDHLF 130

[Close]

BLAST of Lsi03G012970.1 vs. NCBI nr
Match: KAA0051280.1 (photosynthetic NDH subunit of subcomplex B 2 [Cucumis melo var. makuwa] >TYK29981.1 photosynthetic NDH subunit of subcomplex B 2 [Cucumis melo var. makuwa])

HSP 1 Score: 223.0 bits (567), Expect = 2.3e-54
Identity = 118/130 (90.77%), Postives = 123/130 (94.62%), Query Frame = 0


Query: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAAD 60
           MASESGPAVPALPLQDRVAIVTGASRGIGR IALHLA LGAR+V+NYV SSAEAD+V A 
Sbjct: 365 MASESGPAVPALPLQDRVAIVTGASRGIGRGIALHLAGLGARVVVNYVASSAEADQVVAS 424

Query: 61  INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INS+SAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD
Sbjct: 425 INSTSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 484

Query: 121 TSLEIFDQIF 131
           T LEIFD +F
Sbjct: 485 TPLEIFDHLF 494

[Close]

BLAST of Lsi03G012970.1 vs. NCBI nr
Match: XP_008465323.1 (PREDICTED: short-chain type dehydrogenase/reductase-like [Cucumis melo])

HSP 1 Score: 223.0 bits (567), Expect = 2.3e-54
Identity = 118/130 (90.77%), Postives = 123/130 (94.62%), Query Frame = 0


Query: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAAD 60
           MASESGPAVPALPLQDRVAIVTGASRGIGR IALHLA LGAR+V+NYV SSAEAD+V A 
Sbjct: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRGIALHLAGLGARVVVNYVASSAEADQVVAS 60

Query: 61  INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120
           INS+SAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD
Sbjct: 61  INSTSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIAD 120

Query: 121 TSLEIFDQIF 131
           T LEIFD +F
Sbjct: 121 TPLEIFDHLF 130

[Close]

BLAST of Lsi03G012970.1 vs. NCBI nr
Match: XP_038905340.1 (NADPH-dependent aldehyde reductase-like protein, chloroplastic [Benincasa hispida])

HSP 1 Score: 222.2 bits (565), Expect = 4.0e-54
Identity = 121/132 (91.67%), Postives = 123/132 (93.18%), Query Frame = 0


Query: 1   MASESGPAV--PALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVA 60
           M  ESGPAV  PALPLQDRV IVTGASRGIGRAIALHLA LGARLV+NYV  SAEADRV 
Sbjct: 22  MTLESGPAVPLPALPLQDRVTIVTGASRGIGRAIALHLAALGARLVVNYVACSAEADRVV 81

Query: 61  ADINSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYI 120
           A+INSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYI
Sbjct: 82  ANINSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYI 141

Query: 121 ADTSLEIFDQIF 131
           ADTSLEIFDQIF
Sbjct: 142 ADTSLEIFDQIF 153

[Close]

BLAST of Lsi03G012970.1 vs. TAIR 10
Match: AT3G03980.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 146.7 bits (369), Expect = 2.0e-35
Identity = 82/134 (61.19%), Postives = 100/134 (74.63%), Query Frame = 0


Query: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAAD 60
           M++ S  + P LPL  RVAIVTG+SRGIGRAIA+HLA LGAR+VINY + +A+A+RVA++
Sbjct: 1   MSTHSSISQPPLPLAGRVAIVTGSSRGIGRAIAIHLAELGARIVINYTSKAADAERVASE 60

Query: 61  INS----SSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYP 120
           IN         G   RAI  +A+VS+P QVKS+FDAAE AF + VHILVNSAGI DP YP
Sbjct: 61  INDFPVREEITGKGPRAIVVQANVSEPSQVKSMFDAAESAFEAPVHILVNSAGILDPKYP 120

Query: 121 YIADTSLEIFDQIF 131
            IADTS+E FD  F
Sbjct: 121 TIADTSVEDFDHTF 134

[Close]

BLAST of Lsi03G012970.1 vs. TAIR 10
Match: AT5G18210.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 145.6 bits (366), Expect = 4.4e-35
Identity = 77/118 (65.25%), Postives = 95/118 (80.51%), Query Frame = 0


Query: 14  LQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAADINSSSAAGSSQRA 73
           L  RVAIVTG+SRGIGRAIA+HLA LGA++VINY T S EAD+VAA+INSS+       A
Sbjct: 8   LAGRVAIVTGSSRGIGRAIAIHLAELGAKIVINYTTRSTEADQVAAEINSSAGTVPQPIA 67

Query: 74  IAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIADTSLEIFDQIFR 132
           + + AD+S+P Q+KSLFDAAE+AF S VHILVNSAGI +P YP IA+T +E FD+IF+
Sbjct: 68  VVFLADISEPSQIKSLFDAAEKAFNSPVHILVNSAGILNPNYPTIANTPIEEFDRIFK 125

[Close]

BLAST of Lsi03G012970.1 vs. TAIR 10
Match: AT3G04000.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 134.8 bits (338), Expect = 7.8e-32
Identity = 77/134 (57.46%), Postives = 97/134 (72.39%), Query Frame = 0


Query: 1   MASESGPAVPALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAAD 60
           MA+ S  + P L L  RVAIVTG+SRGIGRAIA+HLA LGAR+V+NY TS  EA++VA  
Sbjct: 1   MAAASSVSSPPLCLAGRVAIVTGSSRGIGRAIAIHLAELGARVVVNYSTSPVEAEKVATA 60

Query: 61  INS-----SSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTY 120
           I +     +  AG S R I  +AD+S+P QVKSLFD AE+ F S VHILVNSA I+DP +
Sbjct: 61  ITTNCSKDAEVAGKSPRVIVVKADISEPSQVKSLFDEAERVFESPVHILVNSAAIADPNH 120

Query: 121 PYIADTSLEIFDQI 130
             I+D S+E+FD+I
Sbjct: 121 STISDMSVELFDRI 134

[Close]

BLAST of Lsi03G012970.1 vs. TAIR 10
Match: AT4G13180.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 130.6 bits (327), Expect = 1.5e-30
Identity = 69/120 (57.50%), Postives = 93/120 (77.50%), Query Frame = 0


Query: 11  ALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAADINSSSAAGSS 70
           +LPL  RVAIVTGA+RG+GR IA+HL  LGAR+ INYV+SS++A+ + +++N SS   S 
Sbjct: 10  SLPLAGRVAIVTGATRGMGREIAIHLHSLGARVTINYVSSSSKAELLVSELNDSSQLKS- 69

Query: 71  QRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPTYPYIADTSLEIFDQIF 130
             AIA +ADVSDP+Q+ +LFD  EQ FGS+VHI+VN AG+ DP YP +++T+LE FD  F
Sbjct: 70  --AIAVKADVSDPDQINNLFDQTEQEFGSKVHIVVNCAGVLDPKYPSLSETTLEDFDNTF 126

[Close]

BLAST of Lsi03G012970.1 vs. TAIR 10
Match: AT1G24360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 6.4e-10
Identity = 40/113 (35.40%), Postives = 63/113 (55.75%), Query Frame = 0


Query: 2   ASESGPAVPALPLQDRVAIVTGASRGIGRAIALHLAVLGARLVINYVTSSAEADRVAADI 61
           A+E  P      ++  V ++TGASRGIG+AIAL L   G ++++NY  S+ EA+ VA  I
Sbjct: 62  ATEQSPGEVVQKVESPVVVITGASRGIGKAIALALGKAGCKVLVNYARSAKEAEEVAKQI 121

Query: 62  NSSSAAGSSQRAIAWRADVSDPEQVKSLFDAAEQAFGSQVHILVNSAGISDPT 115
                     +AI +  DVS    V ++   A   +G+ + ++VN+AGI+  T
Sbjct: 122 EEYGG-----QAITFGGDVSKATDVDAMMKTALDKWGT-IDVVVNNAGITRDT 168

The following BLAST results are available for this feature:

BLAST of Lsi03G012970.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SQR4	2.8e-34	61.19	NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Arabidopsis th...	[more]
Q9SQR2	1.1e-30	57.46	NADPH-dependent aldehyde reductase 2, chloroplastic OS=Arabidopsis thaliana OX=3...	[more]
Q08632	2.0e-24	54.55	Short-chain type dehydrogenase/reductase OS=Picea abies OX=3329 PE=2 SV=1	[more]
P0CU75	1.9e-14	40.77	Short chain dehydrogenase claC OS=Passalora fulva OX=5499 GN=claC PE=1 SV=1	[more]
W3XC32	2.7e-13	39.32	Trihydroxynaphthalene reductase PfmaI OS=Pestalotiopsis fici (strain W106-1 / CG...	[more]

BLAST of Lsi03G012970.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LI83	3.0e-55	91.54	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G843800 PE=4 SV=1	[more]
A0A5A7U7W3	1.1e-54	90.77	Photosynthetic NDH subunit of subcomplex B 2 OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A1S3CP09	1.1e-54	90.77	short-chain type dehydrogenase/reductase-like OS=Cucumis melo OX=3656 GN=LOC1035...	[more]
A0A6J1F321	1.5e-51	85.38	NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Cucurbita mosc...	[more]
A0A6J1I5H5	2.9e-50	83.85	NADPH-dependent aldehyde reductase-like protein, chloroplastic OS=Cucurbita maxi...	[more]

BLAST of Lsi03G012970.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN59874.2	6.2e-55	91.54	hypothetical protein Csa_001420 [Cucumis sativus]	[more]
XP_004141209.1	6.2e-55	91.54	NADPH-dependent aldehyde reductase-like protein, chloroplastic [Cucumis sativus]	[more]
KAA0051280.1	2.3e-54	90.77	photosynthetic NDH subunit of subcomplex B 2 [Cucumis melo var. makuwa] >TYK2998...	[more]
XP_008465323.1	2.3e-54	90.77	PREDICTED: short-chain type dehydrogenase/reductase-like [Cucumis melo]	[more]
XP_038905340.1	4.0e-54	91.67	NADPH-dependent aldehyde reductase-like protein, chloroplastic [Benincasa hispid...	[more]

BLAST of Lsi03G012970.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G03980.1	2.0e-35	61.19	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G18210.1	4.4e-35	65.25	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G04000.1	7.8e-32	57.46	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G13180.1	1.5e-30	57.50	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G24360.1	6.4e-10	35.40	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 99..110 score: 42.09 coord: 18..35 score: 54.68
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 18..130 e-value: 3.6E-19 score: 69.0
None	No IPR available	GENE3D	3.40.50.720		coord: 3..133 e-value: 6.3E-31 score: 109.5
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 14..112 e-value: 4.1E-4 score: 17.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 146..163
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 146..205
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 188..205
None	No IPR available	PANTHER	PTHR48107	NADPH-DEPENDENT ALDEHYDE REDUCTASE-LIKE PROTEIN, CHLOROPLASTIC-RELATED	coord: 1..131
None	No IPR available	PANTHER	PTHR48107:SF19	SHORT-CHAIN DEHYDROGENASE/REDUCTASE	coord: 1..131
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 12..131

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi03G012970	Lsi03G012970	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi03G012970.1.exon.2	Lsi03G012970.1.exon.2	exon
Lsi03G012970.1.exon.1	Lsi03G012970.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi03G012970.1.CDS.2	Lsi03G012970.1.CDS.2	CDS
Lsi03G012970.1.CDS.1	Lsi03G012970.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi03G012970.1	Lsi03G012970.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0000724	double-strand break repair via homologous recombination
biological_process	GO:0006303	double-strand break repair via nonhomologous end joining
biological_process	GO:0042138	meiotic DNA double-strand break formation
biological_process	GO:0097552	mitochondrial double-strand break repair via homologous recombination
biological_process	GO:0007095	mitotic G2 DNA damage checkpoint signaling
biological_process	GO:0000723	telomere maintenance
cellular_component	GO:0030870	Mre11 complex
cellular_component	GO:0035861	site of double-strand break
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0008296	3'-5'-exodeoxyribonuclease activity
molecular_function	GO:0030246	carbohydrate binding
molecular_function	GO:0030145	manganese ion binding
molecular_function	GO:0000014	single-stranded DNA endodeoxyribonuclease activity