Lsi03G012430.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G012430.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Germin-like protein
Location	chr03: 23033039 .. 23033870 (-)
Sequence length	561
RNA-Seq Expression	Lsi03G012430.1
Synteny	Lsi03G012430.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAAACCATCATCAGCTTCTTCCTTAATTTTTCTTCTGCTGTTTGTGTTGTTTTCTTGTCTTGGAATCTCAGTAGCAGATTCTAATAATGTCTTTGACACTTGTCCAACAGCCCCAAACAGAACTCAGTTCATCAATGGCTACCATTGCAAAAGCCCCACCCTTGTTACAACTTCTGACTTCAACTCAGCTAAGTTAACGGGACCGATCAATACCGATAATCGATCAAACTTGTTGATGAAGATTGTCACTGCTGCAGATTTCCCTGGTCTGAATACATTGGGGCTTTCTATTTCTAGAACTGATTTGGGTATGGACGCTATGGTTTCTCTTCACTCGCACCCGAGAGCCACCGAGATGTTCTTTCTGAACCAAGGTACGTATTTCTCAAAGTACCGAAATAAGATTCAATTCATTGAAACTCATTAACTTAAACTTTTGGGTTAAGTGATGATCTAACATGCTTTCAAAGTTGGAAGTCCTGTGTTCTCATCTCTTTCGAGAAATATGATTCGCAGGTATTGTTCTAGCTGGATTCATTGATACCAAGAATCAACTGTTTCAGACTGTTCTAAAGGCAGGCGATGTGATTGTATTTCCAAAAGGGTTGTTGCACTTCTGTTTGAATTCTGGTTACGAGCCTGCCATAAGTTTCTCAGTGTACAATAGTCAGAATCCAGGAGTTGTGATCCTCAACGGCGCCATGTTTGATCCTGCTGATCCGAGTCATTTCATAGACAAGTTCCTTAAGAGACTAAAAGCTCTTTCTTCACAGTCAAATTTCAAATTCAACCCAAATGTAACTCTTCCTAGATACACAAGTTTGTAA

mRNA sequence

ATGAAGAAACCATCATCAGCTTCTTCCTTAATTTTTCTTCTGCTGTTTGTGTTGTTTTCTTGTCTTGGAATCTCAGTAGCAGATTCTAATAATGTCTTTGACACTTGTCCAACAGCCCCAAACAGAACTCAGTTCATCAATGGCTACCATTGCAAAAGCCCCACCCTTGTTACAACTTCTGACTTCAACTCAGCTAAGTTAACGGGACCGATCAATACCGATAATCGATCAAACTTGTTGATGAAGATTGTCACTGCTGCAGATTTCCCTGGTCTGAATACATTGGGGCTTTCTATTTCTAGAACTGATTTGGGCGATGTGATTGTATTTCCAAAAGGGTTGTTGCACTTCTGTTTGAATTCTGGTTACGAGCCTGCCATAAGTTTCTCAGTGTACAATAGTCAGAATCCAGGAGTTGTGATCCTCAACGGCGCCATGTTTGATCCTGCTGATCCGAGTCATTTCATAGACAAGTTCCTTAAGAGACTAAAAGCTCTTTCTTCACAGTCAAATTTCAAATTCAACCCAAATGTAACTCTTCCTAGATACACAAGTTTGTAA

Coding sequence (CDS)

ATGAAGAAACCATCATCAGCTTCTTCCTTAATTTTTCTTCTGCTGTTTGTGTTGTTTTCTTGTCTTGGAATCTCAGTAGCAGATTCTAATAATGTCTTTGACACTTGTCCAACAGCCCCAAACAGAACTCAGTTCATCAATGGCTACCATTGCAAAAGCCCCACCCTTGTTACAACTTCTGACTTCAACTCAGCTAAGTTAACGGGACCGATCAATACCGATAATCGATCAAACTTGTTGATGAAGATTGTCACTGCTGCAGATTTCCCTGGTCTGAATACATTGGGGCTTTCTATTTCTAGAACTGATTTGGGCGATGTGATTGTATTTCCAAAAGGGTTGTTGCACTTCTGTTTGAATTCTGGTTACGAGCCTGCCATAAGTTTCTCAGTGTACAATAGTCAGAATCCAGGAGTTGTGATCCTCAACGGCGCCATGTTTGATCCTGCTGATCCGAGTCATTTCATAGACAAGTTCCTTAAGAGACTAAAAGCTCTTTCTTCACAGTCAAATTTCAAATTCAACCCAAATGTAACTCTTCCTAGATACACAAGTTTGTAA

Protein sequence

MKKPSSASSLIFLLLFVLFSCLGISVADSNNVFDTCPTAPNRTQFINGYHCKSPTLVTTSDFNSAKLTGPINTDNRSNLLMKIVTAADFPGLNTLGLSISRTDLGDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNPGVVILNGAMFDPADPSHFIDKFLKRLKALSSQSNFKFNPNVTLPRYTSL

Homology

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BLAST of Lsi03G012430.1 vs. ExPASy Swiss-Prot
Match: Q9FLT3 (Germin-like protein subfamily 3 member 4 OS=Arabidopsis thaliana OX=3702 GN=At5g61750 PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 1.5e-31
Identity = 78/170 (45.88%), Postives = 96/170 (56.47%), Query Frame = 0


Query: 17  VLFSCLGISVA-DSNNVFDTCPTAPNRTQ--FINGYHCKSPTLVTTSDFNSAKLTGPINT 76
           ++F  + +SV+ DS+N+ DTCPTAP      FINGY CK+PT +T  DF S KLT   +T
Sbjct: 7   IVFCAIFLSVSGDSDNMQDTCPTAPGEQSIFFINGYPCKNPTKITAQDFKSTKLTEAGDT 66

Query: 77  DNRSNLLMKIVTAADFPGLNTLGLSISRTDL----------------------------- 136
           DN     + ++TA +FPGLNTLGLS+SRTDL                             
Sbjct: 67  DNYLQSNVTLLTALEFPGLNTLGLSVSRTDLERDGSVPFHSHPRSSEMLFVVKGVVFAGF 126

Query: 137 --------------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNPGVV 141
                         GDV VFPKGLLHFCL+ G+EPA +FS YNSQNPGVV
Sbjct: 127 VDTNNKIFQTVLQKGDVFVFPKGLLHFCLSGGFEPATAFSFYNSQNPGVV 176

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BLAST of Lsi03G012430.1 vs. ExPASy Swiss-Prot
Match: Q2R352 (Germin-like protein 11-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0537350 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.4e-23
Identity = 70/183 (38.25%), Postives = 92/183 (50.27%), Query Frame = 0


Query: 12  FLLLFVLFSCLGISVADSNNVFDTCPTAP--NRTQFINGYHCKSPTLVTTSDFNSAKLTG 71
           +LLL  LF+   IS  DS  + D CP AP   R  F+NG+ CKSP+ +  SDF +  L  
Sbjct: 10  YLLLLGLFAPEIIS--DSPPLQDVCPMAPQGERKLFMNGFFCKSPSTIMASDFKTLLLNH 69

Query: 72  PINTDNRSNLLMKIVTAADFPGLNTLGLSISRTDL------------------------- 131
             + DN       I+TA +FPGLNTLG+S++RTD+                         
Sbjct: 70  AGDLDNMVRSSANIITATEFPGLNTLGISMARTDIAVSGAVLPHSHPRASEMMFVHSGSV 129

Query: 132 ------------------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNPGVVILNGAM 150
                             GDV +FP+GL+HF +N G+  A +FSV NSQNPGVV +  AM
Sbjct: 130 VAGFFDTKGKLFQKTLAEGDVFIFPRGLVHFIMNYGFGLATTFSVLNSQNPGVVGITHAM 189

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BLAST of Lsi03G012430.1 vs. ExPASy Swiss-Prot
Match: P45852 (Germin-like protein OS=Mesembryanthemum crystallinum OX=3544 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 9.7e-18
Identity = 55/158 (34.81%), Postives = 68/158 (43.04%), Query Frame = 0


Query: 40  PNRTQFINGYHCKSPTLVTTSDFNSAKLTGPINTDNRSNLLMKIVTAADFPGLNTLGLSI 99
           PN   F+NG  CK P  V   DF    L  P NTDN       +VTAA+ PGLNTLG+S+
Sbjct: 38  PNDGLFVNGLFCKDPMEVNPDDFLFRGLNMPANTDNALGFAATLVTAANLPGLNTLGISV 97

Query: 100 SRTDL------------------------------------------------GDVIVFP 150
           +R D                                                 GDV VFP
Sbjct: 98  ARLDFAPHGLNPPHTHPRATEVFVVLEGTFYVGFVTSNLADGGNKLFAKVLNKGDVFVFP 157

[Close]

BLAST of Lsi03G012430.1 vs. ExPASy Swiss-Prot
Match: Q7XSN6 (Germin-like protein 4-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0617900 PE=2 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 1.8e-16
Identity = 55/175 (31.43%), Postives = 76/175 (43.43%), Query Frame = 0


Query: 27  ADSNNVFDTCPTAPNRTQFINGYHCKSPTLVTTSDFNSAKLTG-PINTDNRSNLLMKIVT 86
           +D + + D C        F+NG+ CK+P  VT +DF   K  G P NT N     +  VT
Sbjct: 28  SDPSQLQDFCVADKLSAVFVNGFVCKNPKQVTANDFFLPKALGVPGNTVNAQGSAVTPVT 87

Query: 87  AADFPGLNTLGLSISRTDL----------------------------------------- 146
             + PGLNTLG+S +R D                                          
Sbjct: 88  VNELPGLNTLGISFARIDFAPNGQNPPHTHPRATEILTVLQGTLLVGFVTSNQPGGGNLQ 147

Query: 147 -------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNPGVVILNGAMFDPADP 153
                  GDV VFP+GL+HF LN+G  PA++ +  +SQNPGV+ +  A+F    P
Sbjct: 148 FTKLLGPGDVFVFPQGLIHFQLNNGAVPAVAIAALSSQNPGVITIANAVFGSTPP 202

[Close]

BLAST of Lsi03G012430.1 vs. ExPASy Swiss-Prot
Match: Q6ZCR3 (Germin-like protein 8-12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0231400 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 3.1e-16
Identity = 63/194 (32.47%), Postives = 90/194 (46.39%), Query Frame = 0


Query: 7   ASSLIFLL--LFVLFSCLGISVADSNNVFDTCPTAPNRTQFINGYHCKSPTLVTTSD-FN 66
           ASS +FLL  L VL S   I   D + + D C    N    +NG+ CK+P  V++ D FN
Sbjct: 2   ASSSLFLLGALLVLASWQAIVAYDPSPLQDFCVADMNSPVRVNGFACKNPMDVSSEDFFN 61

Query: 67  SAKLTGPINTDNRSNLLMKIVTAADFPGLNTLGLSISRTDL------------------- 126
           +AK   P NT N+    +  +   +FPGLNTLG+S++R D                    
Sbjct: 62  AAKFDMPRNTFNKLGSNVTNLNVMEFPGLNTLGISLARIDYAPMGVNPPHIHPRATELLT 121

Query: 127 -------------------------GDVIVFPKGLLHFCLNSGYE-PAISFSVYNSQNPG 153
                                    GDV VFPK ++HF +N  ++ PA++ S  +SQNPG
Sbjct: 122 VLEGTLYVGFVTSNPNKLFSKVVCKGDVFVFPKAMIHFQMNLDHDKPAVAQSALSSQNPG 181

[Close]

BLAST of Lsi03G012430.1 vs. ExPASy TrEMBL
Match: A0A5D3DCK1 (Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1199G00230 PE=3 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.6e-79
Identity = 162/229 (70.74%), Postives = 172/229 (75.11%), Query Frame = 0


Query: 1   MKKPSSASSLIFLLLFVLFSCLGISVADSNNVFDTCPTAPNRTQFINGYHCKSPTLVTTS 60
           MK PS ASSLIFLLLFVLFSCLGIS+ADSNNVFDTCPTA +R +FINGYHCKSPTLVTTS
Sbjct: 1   MKNPSLASSLIFLLLFVLFSCLGISLADSNNVFDTCPTATDRIEFINGYHCKSPTLVTTS 60

Query: 61  DFNSAKLTGPINTDNRSNLLMKIVTAADFPGLNTLGLSISRTDL---------------- 120
           DFNSAKLT P+NTD+R NL++KIVTAADF GLNTLGLSISRTDL                
Sbjct: 61  DFNSAKLTEPVNTDDRINLVVKIVTAADFHGLNTLGLSISRTDLVIDAIVSPHSHPRATE 120

Query: 121 ---------------------------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP 180
                                      GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP
Sbjct: 121 MFFLNQGIVLAGFIDTRNQLFQTILKAGDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP 180

Query: 181 GVVILNGAMFDPADPSHFIDKFLKRLKALSSQSNFKFNPNVTLPRYTSL 187
           GVV +NGA+FDPAD SHFI+KFLKRL   SSQSNFK NPNVTL RYTSL
Sbjct: 181 GVVNINGAVFDPADSSHFIEKFLKRLTVPSSQSNFKLNPNVTLSRYTSL 229

[Close]

BLAST of Lsi03G012430.1 vs. ExPASy TrEMBL
Match: A0A1S3CNX7 (Germin-like protein OS=Cucumis melo OX=3656 GN=LOC103503059 PE=3 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.6e-79
Identity = 162/229 (70.74%), Postives = 172/229 (75.11%), Query Frame = 0


Query: 1   MKKPSSASSLIFLLLFVLFSCLGISVADSNNVFDTCPTAPNRTQFINGYHCKSPTLVTTS 60
           MK PS ASSLIFLLLFVLFSCLGIS+ADSNNVFDTCPTA +R +FINGYHCKSPTLVTTS
Sbjct: 1   MKNPSLASSLIFLLLFVLFSCLGISLADSNNVFDTCPTATDRIEFINGYHCKSPTLVTTS 60

Query: 61  DFNSAKLTGPINTDNRSNLLMKIVTAADFPGLNTLGLSISRTDL---------------- 120
           DFNSAKLT P+NTD+R NL++KIVTAADF GLNTLGLSISRTDL                
Sbjct: 61  DFNSAKLTEPVNTDDRINLVVKIVTAADFHGLNTLGLSISRTDLVIDAIVSPHSHPRATE 120

Query: 121 ---------------------------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP 180
                                      GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP
Sbjct: 121 MFFLNQGIVLAGFIDTRNQLFQTILKAGDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP 180

Query: 181 GVVILNGAMFDPADPSHFIDKFLKRLKALSSQSNFKFNPNVTLPRYTSL 187
           GVV +NGA+FDPAD SHFI+KFLKRL   SSQSNFK NPNVTL RYTSL
Sbjct: 181 GVVNINGAVFDPADSSHFIEKFLKRLTVPSSQSNFKLNPNVTLSRYTSL 229

[Close]

BLAST of Lsi03G012430.1 vs. ExPASy TrEMBL
Match: A0A6J1DJ19 (Germin-like protein OS=Momordica charantia OX=3673 GN=LOC111020943 PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 1.9e-69
Identity = 145/229 (63.32%), Postives = 167/229 (72.93%), Query Frame = 0


Query: 1   MKKPSSASSLIFLLLFVLFSCLGISVADSNNVFDTCPTAPNRTQFINGYHCKSPTLVTTS 60
           MKKPSS SSLIFLLLFVLFSCLGISVADSNNVFDTCPTA N+++FING+ CK+PT+VTTS
Sbjct: 1   MKKPSS-SSLIFLLLFVLFSCLGISVADSNNVFDTCPTATNKSEFINGFPCKNPTIVTTS 60

Query: 61  DFNSAKLTGPINTDNRSNLLMKIVTAADFPGLNTLGLSISRTDL---------------- 120
           DFNSAKLTGP++TD++    + I+TAA+F GLNTLGLSISRTDL                
Sbjct: 61  DFNSAKLTGPVDTDDQFISSVNILTAAEFSGLNTLGLSISRTDLGTDAIVSPHSHPRATE 120

Query: 121 ---------------------------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP 180
                                      GDV+VFPKGLLHFCLNSGYEPAI FSV+NSQNP
Sbjct: 121 IFFVNQGIVLAGFLDTKNRLFQSFLKAGDVMVFPKGLLHFCLNSGYEPAIIFSVFNSQNP 180

Query: 181 GVVILNGAMFDPADPSHFIDKFLKRLKALSSQSNFKFNPNVTLPRYTSL 187
           GVV +NGAMF+PAD +H + KFL+RLK+LSS S+ KFNPNVTL  Y SL
Sbjct: 181 GVVNINGAMFEPADSNHLVHKFLERLKSLSSLSSLKFNPNVTLTTYISL 228

[Close]

BLAST of Lsi03G012430.1 vs. ExPASy TrEMBL
Match: A0A6J1GAI2 (Germin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111452269 PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 6.2e-68
Identity = 146/229 (63.76%), Postives = 161/229 (70.31%), Query Frame = 0


Query: 1   MKKPSSASSLIFLLLFVLFSCLGISVADSNNVFDTCPTAPNRTQFINGYHCKSPTLVTTS 60
           M KPS  SSLIFLL+ V FSCLGISVADS+NVFD+CPTA +R  FING+ CK+PT+V T 
Sbjct: 1   MNKPS--SSLIFLLV-VFFSCLGISVADSHNVFDSCPTATDRIDFINGFPCKNPTIVRTD 60

Query: 61  DFNSAKLTGPINTDNRSNLLMKIVTAADFPGLNTLGLSISRTDL---------------- 120
           DF SAKLTGP+NTD+R + L+K+VTAADFPGLN+LGLSISRTDL                
Sbjct: 61  DFTSAKLTGPVNTDDRFSSLVKVVTAADFPGLNSLGLSISRTDLGTDAIVSPHSHPRATE 120

Query: 121 ---------------------------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP 180
                                      GDVIVFPKGLLHFCLNSGYE A  FSVYNSQNP
Sbjct: 121 MFFVNQGIVLAGFIDTKNRLFQEFLKPGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNP 180

Query: 181 GVVILNGAMFDPADPSHFIDKFLKRLKALSSQSNFKFNPNVTLPRYTSL 187
           GVV +NGAMF PAD SHFIDKFL +LKALSS  N KFNPNVTLPRY SL
Sbjct: 181 GVVNVNGAMFKPADSSHFIDKFLDKLKALSSLPNLKFNPNVTLPRYISL 226

[Close]

BLAST of Lsi03G012430.1 vs. ExPASy TrEMBL
Match: A0A6J1KCN7 (Germin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111493105 PE=3 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 2.4e-67
Identity = 144/229 (62.88%), Postives = 161/229 (70.31%), Query Frame = 0


Query: 1   MKKPSSASSLIFLLLFVLFSCLGISVADSNNVFDTCPTAPNRTQFINGYHCKSPTLVTTS 60
           M KPS  SSLIFLLL V FSCLGI+VADS+NVFD+CPTA +R  F+NG+ CK+PT+V T 
Sbjct: 1   MNKPS--SSLIFLLL-VFFSCLGIAVADSHNVFDSCPTATDRIDFVNGFPCKNPTVVRTD 60

Query: 61  DFNSAKLTGPINTDNRSNLLMKIVTAADFPGLNTLGLSISRTDL---------------- 120
           DF SAKLTGP+NTD+R + L+K+VTAADFPGLN+LGLSISRTDL                
Sbjct: 61  DFTSAKLTGPVNTDDRFSSLVKVVTAADFPGLNSLGLSISRTDLGTDAIVSPHSHPRATE 120

Query: 121 ---------------------------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP 180
                                      GDVIVFPKGLLHFCLNSGYE A  FSVYNSQNP
Sbjct: 121 MFFVNQGIVLAGFIDTKNRLFQEFLKPGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNP 180

Query: 181 GVVILNGAMFDPADPSHFIDKFLKRLKALSSQSNFKFNPNVTLPRYTSL 187
           GVV +NGAMF+PAD SHFID FL +LKALSS  N KFNPNVTLPRY SL
Sbjct: 181 GVVNVNGAMFEPADSSHFIDMFLDKLKALSSLPNLKFNPNVTLPRYISL 226

[Close]

BLAST of Lsi03G012430.1 vs. NCBI nr
Match: XP_038905670.1 (germin-like protein subfamily 3 member 4 [Benincasa hispida])

HSP 1 Score: 327.8 bits (839), Expect = 6.1e-86
Identity = 172/229 (75.11%), Postives = 180/229 (78.60%), Query Frame = 0


Query: 1   MKKPSSASSLIFLLLFVLFSCLGISVADSNNVFDTCPTAPNRTQFINGYHCKSPTLVTTS 60
           MK PSSASSLIFLLLFVLF+CLGISVADSNNVFDTCPTA +R +FINGYHCKSPTLVTTS
Sbjct: 15  MKNPSSASSLIFLLLFVLFACLGISVADSNNVFDTCPTATDRIEFINGYHCKSPTLVTTS 74

Query: 61  DFNSAKLTGPINTDNRSNLLMKIVTAADFPGLNTLGLSISRTDL---------------- 120
           DFNSAKLTGPIN+D++ NLL+KIVTAADFPGLNTLGLSISRTDL                
Sbjct: 75  DFNSAKLTGPINSDDQFNLLVKIVTAADFPGLNTLGLSISRTDLGTDAIISPHSHPRATE 134

Query: 121 ---------------------------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP 180
                                      GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP
Sbjct: 135 MFFLNQGIVLAGFIDTRNQLFQTILKAGDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP 194

Query: 181 GVVILNGAMFDPADPSHFIDKFLKRLKALSSQSNFKFNPNVTLPRYTSL 187
           GVV +NGAMFDPAD SHFIDKFLKRLKALSSQSNFKFNPNVTLPRYTSL
Sbjct: 195 GVVNINGAMFDPADSSHFIDKFLKRLKALSSQSNFKFNPNVTLPRYTSL 243

[Close]

BLAST of Lsi03G012430.1 vs. NCBI nr
Match: XP_008465454.1 (PREDICTED: germin-like protein subfamily 3 member 4 [Cucumis melo] >KAA0034959.1 germin-like protein subfamily 3 member 4 [Cucumis melo var. makuwa] >TYK21314.1 germin-like protein subfamily 3 member 4 [Cucumis melo var. makuwa])

HSP 1 Score: 305.4 bits (781), Expect = 3.2e-79
Identity = 162/229 (70.74%), Postives = 172/229 (75.11%), Query Frame = 0


Query: 1   MKKPSSASSLIFLLLFVLFSCLGISVADSNNVFDTCPTAPNRTQFINGYHCKSPTLVTTS 60
           MK PS ASSLIFLLLFVLFSCLGIS+ADSNNVFDTCPTA +R +FINGYHCKSPTLVTTS
Sbjct: 1   MKNPSLASSLIFLLLFVLFSCLGISLADSNNVFDTCPTATDRIEFINGYHCKSPTLVTTS 60

Query: 61  DFNSAKLTGPINTDNRSNLLMKIVTAADFPGLNTLGLSISRTDL---------------- 120
           DFNSAKLT P+NTD+R NL++KIVTAADF GLNTLGLSISRTDL                
Sbjct: 61  DFNSAKLTEPVNTDDRINLVVKIVTAADFHGLNTLGLSISRTDLVIDAIVSPHSHPRATE 120

Query: 121 ---------------------------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP 180
                                      GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP
Sbjct: 121 MFFLNQGIVLAGFIDTRNQLFQTILKAGDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP 180

Query: 181 GVVILNGAMFDPADPSHFIDKFLKRLKALSSQSNFKFNPNVTLPRYTSL 187
           GVV +NGA+FDPAD SHFI+KFLKRL   SSQSNFK NPNVTL RYTSL
Sbjct: 181 GVVNINGAVFDPADSSHFIEKFLKRLTVPSSQSNFKLNPNVTLSRYTSL 229

[Close]

BLAST of Lsi03G012430.1 vs. NCBI nr
Match: XP_022153439.1 (germin-like protein subfamily 3 member 4 [Momordica charantia])

HSP 1 Score: 271.9 bits (694), Expect = 4.0e-69
Identity = 145/229 (63.32%), Postives = 167/229 (72.93%), Query Frame = 0


Query: 1   MKKPSSASSLIFLLLFVLFSCLGISVADSNNVFDTCPTAPNRTQFINGYHCKSPTLVTTS 60
           MKKPSS SSLIFLLLFVLFSCLGISVADSNNVFDTCPTA N+++FING+ CK+PT+VTTS
Sbjct: 1   MKKPSS-SSLIFLLLFVLFSCLGISVADSNNVFDTCPTATNKSEFINGFPCKNPTIVTTS 60

Query: 61  DFNSAKLTGPINTDNRSNLLMKIVTAADFPGLNTLGLSISRTDL---------------- 120
           DFNSAKLTGP++TD++    + I+TAA+F GLNTLGLSISRTDL                
Sbjct: 61  DFNSAKLTGPVDTDDQFISSVNILTAAEFSGLNTLGLSISRTDLGTDAIVSPHSHPRATE 120

Query: 121 ---------------------------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP 180
                                      GDV+VFPKGLLHFCLNSGYEPAI FSV+NSQNP
Sbjct: 121 IFFVNQGIVLAGFLDTKNRLFQSFLKAGDVMVFPKGLLHFCLNSGYEPAIIFSVFNSQNP 180

Query: 181 GVVILNGAMFDPADPSHFIDKFLKRLKALSSQSNFKFNPNVTLPRYTSL 187
           GVV +NGAMF+PAD +H + KFL+RLK+LSS S+ KFNPNVTL  Y SL
Sbjct: 181 GVVNINGAMFEPADSNHLVHKFLERLKSLSSLSSLKFNPNVTLTTYISL 228

[Close]

BLAST of Lsi03G012430.1 vs. NCBI nr
Match: XP_023522282.1 (germin-like protein subfamily 3 member 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 269.6 bits (688), Expect = 2.0e-68
Identity = 147/229 (64.19%), Postives = 163/229 (71.18%), Query Frame = 0


Query: 1   MKKPSSASSLIFLLLFVLFSCLGISVADSNNVFDTCPTAPNRTQFINGYHCKSPTLVTTS 60
           M KPS  SSLIFLL+ VLFSCLGISVADS+NVFD+CPTA +R  FING+ CK+PT+V T 
Sbjct: 15  MNKPS--SSLIFLLV-VLFSCLGISVADSHNVFDSCPTATDRIDFINGFPCKNPTIVRTD 74

Query: 61  DFNSAKLTGPINTDNRSNLLMKIVTAADFPGLNTLGLSISRTDL---------------- 120
           DF SAKLTGP+NTD+R + L+K+VTAADFPGLN+LGLSISRTDL                
Sbjct: 75  DFTSAKLTGPVNTDDRFSSLVKVVTAADFPGLNSLGLSISRTDLGTDAIISPHSHPRATE 134

Query: 121 ---------------------------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP 180
                                      GDVIVFPKGLLHFCLNSGYE A  FSVYNSQNP
Sbjct: 135 MFFVNQGIVLAGFIDTKNRLFQEFLKPGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNP 194

Query: 181 GVVILNGAMFDPADPSHFIDKFLKRLKALSSQSNFKFNPNVTLPRYTSL 187
           GVV +NGAMF+PAD SHFIDKFL +LKALSS  N KFNPNVTLPRY SL
Sbjct: 195 GVVNVNGAMFEPADSSHFIDKFLDKLKALSSLPNLKFNPNVTLPRYISL 240

[Close]

BLAST of Lsi03G012430.1 vs. NCBI nr
Match: XP_022948659.1 (germin-like protein subfamily 3 member 4 [Cucurbita moschata] >XP_022948660.1 germin-like protein subfamily 3 member 4 [Cucurbita moschata])

HSP 1 Score: 266.9 bits (681), Expect = 1.3e-67
Identity = 146/229 (63.76%), Postives = 161/229 (70.31%), Query Frame = 0


Query: 1   MKKPSSASSLIFLLLFVLFSCLGISVADSNNVFDTCPTAPNRTQFINGYHCKSPTLVTTS 60
           M KPS  SSLIFLL+ V FSCLGISVADS+NVFD+CPTA +R  FING+ CK+PT+V T 
Sbjct: 1   MNKPS--SSLIFLLV-VFFSCLGISVADSHNVFDSCPTATDRIDFINGFPCKNPTIVRTD 60

Query: 61  DFNSAKLTGPINTDNRSNLLMKIVTAADFPGLNTLGLSISRTDL---------------- 120
           DF SAKLTGP+NTD+R + L+K+VTAADFPGLN+LGLSISRTDL                
Sbjct: 61  DFTSAKLTGPVNTDDRFSSLVKVVTAADFPGLNSLGLSISRTDLGTDAIVSPHSHPRATE 120

Query: 121 ---------------------------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNP 180
                                      GDVIVFPKGLLHFCLNSGYE A  FSVYNSQNP
Sbjct: 121 MFFVNQGIVLAGFIDTKNRLFQEFLKPGDVIVFPKGLLHFCLNSGYETATIFSVYNSQNP 180

Query: 181 GVVILNGAMFDPADPSHFIDKFLKRLKALSSQSNFKFNPNVTLPRYTSL 187
           GVV +NGAMF PAD SHFIDKFL +LKALSS  N KFNPNVTLPRY SL
Sbjct: 181 GVVNVNGAMFKPADSSHFIDKFLDKLKALSSLPNLKFNPNVTLPRYISL 226

[Close]

BLAST of Lsi03G012430.1 vs. TAIR 10
Match: AT5G61750.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 137.5 bits (345), Expect = 1.1e-32
Identity = 78/170 (45.88%), Postives = 96/170 (56.47%), Query Frame = 0


Query: 17  VLFSCLGISVA-DSNNVFDTCPTAPNRTQ--FINGYHCKSPTLVTTSDFNSAKLTGPINT 76
           ++F  + +SV+ DS+N+ DTCPTAP      FINGY CK+PT +T  DF S KLT   +T
Sbjct: 7   IVFCAIFLSVSGDSDNMQDTCPTAPGEQSIFFINGYPCKNPTKITAQDFKSTKLTEAGDT 66

Query: 77  DNRSNLLMKIVTAADFPGLNTLGLSISRTDL----------------------------- 136
           DN     + ++TA +FPGLNTLGLS+SRTDL                             
Sbjct: 67  DNYLQSNVTLLTALEFPGLNTLGLSVSRTDLERDGSVPFHSHPRSSEMLFVVKGVVFAGF 126

Query: 137 --------------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNPGVV 141
                         GDV VFPKGLLHFCL+ G+EPA +FS YNSQNPGVV
Sbjct: 127 VDTNNKIFQTVLQKGDVFVFPKGLLHFCLSGGFEPATAFSFYNSQNPGVV 176

[Close]

BLAST of Lsi03G012430.1 vs. TAIR 10
Match: AT5G20630.1 (germin 3 )

HSP 1 Score: 81.6 bits (200), Expect = 7.1e-16
Identity = 65/212 (30.66%), Postives = 92/212 (43.40%), Query Frame = 0


Query: 13  LLLFVLFSCLGISVADSNNVFDTCPTAPNRTQFINGYHCKSPTLVTTSDFNSAKLTGPIN 72
           +++ + F    IS     +V D C   P   Q  +GY CK+P  VT +DF     TG   
Sbjct: 3   MIIQIFFIISLISTISFASVQDFCVADPKGPQSPSGYSCKNPDQVTENDF---AFTGLGT 62

Query: 73  TDNRSNLLMKIVTAADFP---GLNTLGLSISRTDL------------------------- 132
             N SN++   VT A  P   G+N LG+S++R DL                         
Sbjct: 63  AGNTSNIIKAAVTPAFAPAYAGINGLGVSLARLDLAGGGVIPLHTHPGASEVLVVIQGTI 122

Query: 133 ------------------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNPGVVILNGAM 172
                             GD +VFP+GLLHF LNSG  PA++F  + S +PG+ IL  A+
Sbjct: 123 CAGFISSANKVYLKTLNRGDSMVFPQGLLHFQLNSGKGPALAFVAFGSSSPGLQILPFAL 182

[Close]

BLAST of Lsi03G012430.1 vs. TAIR 10
Match: AT5G38960.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 78.6 bits (192), Expect = 6.0e-15
Identity = 60/195 (30.77%), Postives = 85/195 (43.59%), Query Frame = 0


Query: 13  LLLFVLFSCLGISVA-DSNNVFDTC--PTAPNRTQFINGYHCKSPTLVTTSDFNSAKLTG 72
           +L  +  S L  ++A D + + D C     P    F+NG  CK P LVT  DF  + L  
Sbjct: 8   ILYLLAASTLPFAIASDPSPLQDFCIGVNTPANALFVNGKFCKDPKLVTADDFYFSGLDK 67

Query: 73  PINTDNR---SNLLMKIVTAADFPGLNTLGLSISRTDL---------------------- 132
              T++    SN  +  V     PGLNTLG+S+ R D                       
Sbjct: 68  ARTTESSPVGSN--VTTVNVNQIPGLNTLGISLVRIDYGINGQNPPHTHPRATEILLVQE 127

Query: 133 -----------------------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNPGVVI 154
                                  GDV VFP+GL+HF +N G +PA++F+  +SQNPGV+I
Sbjct: 128 GTLFVGFFSSFPENRLFNKTLNKGDVFVFPEGLIHFQVNIGKQPAVAFASLSSQNPGVII 187

[Close]

BLAST of Lsi03G012430.1 vs. TAIR 10
Match: AT1G09560.1 (germin-like protein 5 )

HSP 1 Score: 75.9 bits (185), Expect = 3.9e-14
Identity = 58/190 (30.53%), Postives = 75/190 (39.47%), Query Frame = 0


Query: 6   SASSLIFLLLFVLFSCLGISVADSNNVFDTCPTAPNRTQFINGYHCKSPTLVTTSDFNSA 65
           ++ +L  LLL    S    S AD + + D C         ING+ CK    VT++DF S 
Sbjct: 2   ASPTLTLLLLLTTVSFFISSSADPDMLQDLCVADLPSGIKINGFPCKDAATVTSADFFSQ 61

Query: 66  KLTGPINTDNRSNLLMKIVTAADFPGLNTLGLSISRTDL--------------------- 125
            L  P  T+N    L+        PGLNTLG+S+SR D                      
Sbjct: 62  GLAKPGLTNNTFGALVTGANVMTIPGLNTLGVSLSRIDYAPGGLNPPHTHPRATEVVFVL 121

Query: 126 ----------------------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNPGVVIL 153
                                 GDV  FPKGL+HF  N+G  PA   + +NSQ PG   L
Sbjct: 122 EGTLDVGFLTTANKLISQSLKKGDVFAFPKGLVHFQKNNGDVPASVIAAFNSQLPGTQSL 181

[Close]

BLAST of Lsi03G012430.1 vs. TAIR 10
Match: AT1G18970.1 (germin-like protein 4 )

HSP 1 Score: 75.5 bits (184), Expect = 5.1e-14
Identity = 54/179 (30.17%), Postives = 79/179 (44.13%), Query Frame = 0


Query: 12  FLLLFVLFSCLGISVADSNNVFDTCPTAPNRTQFINGYHCKSPTLVTTSDFNSAKLTGPI 71
           FLL+F LF    +S +DS+ + D C      +  ING+ CKS   V+ SDF  + L GP+
Sbjct: 14  FLLMFCLFVIPSLS-SDSDPLQDFCVGDLKASASINGFPCKS--AVSASDFFYSGLGGPL 73

Query: 72  NTDNRSNLLMKIVTAADFPGLNTLGLSISRTDL--------------------------- 131
           +T N + + +       FPGLNTLG+S++  +L                           
Sbjct: 74  DTSNPNGVTVAPANVLTFPGLNTLGISMNNVELAPGGVNPPHLHPRATEVGTVIEGSVFV 133

Query: 132 ----------------GDVIVFPKGLLHFCLNSGYEPAISFSVYNSQNPGVVILNGAMF 148
                           G+  V P+GL+HF  N G   A   + +NSQ PG V+L   +F
Sbjct: 134 GFLSTNNTLFSKVLNAGEAFVIPRGLVHFQWNVGQVKARMITAFNSQLPGAVVLPSTLF 189

The following BLAST results are available for this feature:

BLAST of Lsi03G012430.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FLT3	1.5e-31	45.88	Germin-like protein subfamily 3 member 4 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]
Q2R352	3.4e-23	38.25	Germin-like protein 11-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g053735...	[more]
P45852	9.7e-18	34.81	Germin-like protein OS=Mesembryanthemum crystallinum OX=3544 PE=2 SV=1	[more]
Q7XSN6	1.8e-16	31.43	Germin-like protein 4-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0617900...	[more]
Q6ZCR3	3.1e-16	32.47	Germin-like protein 8-12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g023140...	[more]

BLAST of Lsi03G012430.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DCK1	1.6e-79	70.74	Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1199...	[more]
A0A1S3CNX7	1.6e-79	70.74	Germin-like protein OS=Cucumis melo OX=3656 GN=LOC103503059 PE=3 SV=1	[more]
A0A6J1DJ19	1.9e-69	63.32	Germin-like protein OS=Momordica charantia OX=3673 GN=LOC111020943 PE=3 SV=1	[more]
A0A6J1GAI2	6.2e-68	63.76	Germin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111452269 PE=3 SV=1	[more]
A0A6J1KCN7	2.4e-67	62.88	Germin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111493105 PE=3 SV=1	[more]

BLAST of Lsi03G012430.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905670.1	6.1e-86	75.11	germin-like protein subfamily 3 member 4 [Benincasa hispida]	[more]
XP_008465454.1	3.2e-79	70.74	PREDICTED: germin-like protein subfamily 3 member 4 [Cucumis melo] >KAA0034959.1...	[more]
XP_022153439.1	4.0e-69	63.32	germin-like protein subfamily 3 member 4 [Momordica charantia]	[more]
XP_023522282.1	2.0e-68	64.19	germin-like protein subfamily 3 member 4 [Cucurbita pepo subsp. pepo]	[more]
XP_022948659.1	1.3e-67	63.76	germin-like protein subfamily 3 member 4 [Cucurbita moschata] >XP_022948660.1 ge...	[more]

BLAST of Lsi03G012430.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G61750.1	1.1e-32	45.88	RmlC-like cupins superfamily protein	[more]
AT5G20630.1	7.1e-16	30.66	germin 3	[more]
AT5G38960.1	6.0e-15	30.77	RmlC-like cupins superfamily protein	[more]
AT1G09560.1	3.9e-14	30.53	germin-like protein 5	[more]
AT1G18970.1	5.1e-14	30.17	germin-like protein 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001929	Germin	PRINTS	PR00325	GERMIN	coord: 133..148 score: 43.75 coord: 100..120 score: 46.62
IPR006045	Cupin 1	PFAM	PF00190	Cupin_1	coord: 105..141 e-value: 1.1E-8 score: 34.8
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 105..174 e-value: 4.8E-13 score: 50.9 coord: 27..104 e-value: 2.8E-13 score: 51.7
None	No IPR available	PANTHER	PTHR31238	GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3	coord: 105..165
None	No IPR available	PANTHER	PTHR31238:SF3	GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 4	coord: 105..165
None	No IPR available	PANTHER	PTHR31238	GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3	coord: 15..104
None	No IPR available	PANTHER	PTHR31238:SF3	GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 4	coord: 15..104
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..21 score: 6.0
IPR011051	RmlC-like cupin domain superfamily	SUPERFAMILY	51182	RmlC-like cupins	coord: 34..147

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi03G012430	Lsi03G012430	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi03G012430.1.exon.2	Lsi03G012430.1.exon.2	exon
Lsi03G012430.1.exon.1	Lsi03G012430.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi03G012430.1.CDS.2	Lsi03G012430.1.CDS.2	CDS
Lsi03G012430.1.CDS.1	Lsi03G012430.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi03G012430.1	Lsi03G012430.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0030145	manganese ion binding