Lsi02G016380.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi02G016380.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPhorbol-ester/DAG-type domain-containing protein
Locationchr02: 22237930 .. 22238688 (+)
Sequence length759
RNA-Seq ExpressionLsi02G016380.1
SyntenyLsi02G016380.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAAGCAGTTGTGTAATCCAACCTACCTGAAGCATGAACTGGAGCTGAAGAAGTACCTGAAACCTTACACCTGCGATGGCTGCAAGGAAATAGGCTTTGGGCCTCGATACCGATGTGAAAAGTGCAATTTTGATCTTCACAAAGCCTGTATGTTTCCAAAGAGCTCACCAATTTCCCATATATTCTTCCCACGTTCCACCTTCAAATTCTATAGAACCCCACCAAGAGCCTGCCACGATGAATGCAAACGCATCTGTGAAGCCTGCACAAAGCCTATCAATGGCTTTATGTACCACTGCAAGAAAGACGACTTGGATCTTCACCCCTGCTGCCGAAAGCTCAAAAAGAATTACCAAATTGAAGACGTGGAGTTCAATCTTCACAAGAAAGTTAGGGGGAAGTGCATATGGTGCAAAAAGAGGAGCACCAAAAGCAGTGGCGACGACAATGGTTGGTCCTACATATCTGATTGCGGGAAGTACCATGTTCACGTGGCTTGTGTCACTGAAATGGCTTTAGAAGAATGGTACAAGAGCGGAGGAGACTGGATCAATGACAATAATGCCAACCAGGACCAACAACTGGCTCTGCAGAAGGTGAATCTGAAAGCAATTCAAGCACGAGGTCGTGGGGATGGCGGAAAAGGGAACAAGTTTTGGAGAATCCTTAAGGTCTTCATCAAGACAATTGTTAGTATCGTTCTTGGAGATCCCACCACGGTTCTGGCGTCTCTCTTCGTCGAGCTGATTGCATAA

mRNA sequence

ATGGCAAAGCAGTTGTGTAATCCAACCTACCTGAAGCATGAACTGGAGCTGAAGAAGTACCTGAAACCTTACACCTGCGATGGCTGCAAGGAAATAGGCTTTGGGCCTCGATACCGATGTGAAAAGTGCAATTTTGATCTTCACAAAGCCTGTATGTTTCCAAAGAGCTCACCAATTTCCCATATATTCTTCCCACGTTCCACCTTCAAATTCTATAGAACCCCACCAAGAGCCTGCCACGATGAATGCAAACGCATCTGTGAAGCCTGCACAAAGCCTATCAATGGCTTTATGTACCACTGCAAGAAAGACGACTTGGATCTTCACCCCTGCTGCCGAAAGCTCAAAAAGAATTACCAAATTGAAGACGTGGAGTTCAATCTTCACAAGAAAGTTAGGGGGAAGTGCATATGGTGCAAAAAGAGGAGCACCAAAAGCAGTGGCGACGACAATGGTTGGTCCTACATATCTGATTGCGGGAAGTACCATGTTCACGTGGCTTGTGTCACTGAAATGGCTTTAGAAGAATGGTACAAGAGCGGAGGAGACTGGATCAATGACAATAATGCCAACCAGGACCAACAACTGGCTCTGCAGAAGGTGAATCTGAAAGCAATTCAAGCACGAGGTCGTGGGGATGGCGGAAAAGGGAACAAGTTTTGGAGAATCCTTAAGGTCTTCATCAAGACAATTGTTAGTATCGTTCTTGGAGATCCCACCACGGTTCTGGCGTCTCTCTTCGTCGAGCTGATTGCATAA

Coding sequence (CDS)

ATGGCAAAGCAGTTGTGTAATCCAACCTACCTGAAGCATGAACTGGAGCTGAAGAAGTACCTGAAACCTTACACCTGCGATGGCTGCAAGGAAATAGGCTTTGGGCCTCGATACCGATGTGAAAAGTGCAATTTTGATCTTCACAAAGCCTGTATGTTTCCAAAGAGCTCACCAATTTCCCATATATTCTTCCCACGTTCCACCTTCAAATTCTATAGAACCCCACCAAGAGCCTGCCACGATGAATGCAAACGCATCTGTGAAGCCTGCACAAAGCCTATCAATGGCTTTATGTACCACTGCAAGAAAGACGACTTGGATCTTCACCCCTGCTGCCGAAAGCTCAAAAAGAATTACCAAATTGAAGACGTGGAGTTCAATCTTCACAAGAAAGTTAGGGGGAAGTGCATATGGTGCAAAAAGAGGAGCACCAAAAGCAGTGGCGACGACAATGGTTGGTCCTACATATCTGATTGCGGGAAGTACCATGTTCACGTGGCTTGTGTCACTGAAATGGCTTTAGAAGAATGGTACAAGAGCGGAGGAGACTGGATCAATGACAATAATGCCAACCAGGACCAACAACTGGCTCTGCAGAAGGTGAATCTGAAAGCAATTCAAGCACGAGGTCGTGGGGATGGCGGAAAAGGGAACAAGTTTTGGAGAATCCTTAAGGTCTTCATCAAGACAATTGTTAGTATCGTTCTTGGAGATCCCACCACGGTTCTGGCGTCTCTCTTCGTCGAGCTGATTGCATAA

Protein sequence

MAKQLCNPTYLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFPKSSPISHIFFPRSTFKFYRTPPRACHDECKRICEACTKPINGFMYHCKKDDLDLHPCCRKLKKNYQIEDVEFNLHKKVRGKCIWCKKRSTKSSGDDNGWSYISDCGKYHVHVACVTEMALEEWYKSGGDWINDNNANQDQQLALQKVNLKAIQARGRGDGGKGNKFWRILKVFIKTIVSIVLGDPTTVLASLFVELIA
Homology
BLAST of Lsi02G016380.1 vs. ExPASy Swiss-Prot
Match: Q7Y0E8 (Probable nucleoredoxin 1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405500 PE=2 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 7.5e-05
Identity = 24/53 (45.28%), Postives = 28/53 (52.83%), Query Frame = 0

Query: 8   PTYLKHELELKKYL-----KPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFPK 56
           P  LKHEL  +  L       Y CDGC E+G    YRC +C+FDLH  C   K
Sbjct: 491 PGKLKHELHDEHELVLTRCTTYGCDGCDEMGSSWSYRCRECDFDLHPKCALGK 543

BLAST of Lsi02G016380.1 vs. ExPASy Swiss-Prot
Match: Q7Y0F2 (Probable nucleoredoxin 1-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405900 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.7e-04
Identity = 21/56 (37.50%), Postives = 30/56 (53.57%), Query Frame = 0

Query: 8   PTYLKHELELKKYL----KPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFPKSSPI 60
           P  LKH+L   + +      Y CDGC E+G    YRC++C+FDLH  C   +   +
Sbjct: 502 PEKLKHDLHDHELVLTRCTTYGCDGCDEMGDSWSYRCKECDFDLHPKCALEEKGDV 557

BLAST of Lsi02G016380.1 vs. ExPASy TrEMBL
Match: A0A0A0KS98 (Phorbol-ester/DAG-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G517070 PE=4 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 1.8e-131
Identity = 214/252 (84.92%), Postives = 233/252 (92.46%), Query Frame = 0

Query: 1   MAKQLCNPTYLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFPKSSPIS 60
           MAKQLC P  LKHELELKKYLKPYTCDGCKEIGFGPRYRCEKC+FDLHKACMFP+ SP+ 
Sbjct: 1   MAKQLCTPNQLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCDFDLHKACMFPQDSPVF 60

Query: 61  HIFFPRSTFKFYRTPPRACHDECKRICEACTKPINGFMYHCKKDDLDLHPCCRKLKKNYQ 120
           H FF RS FKF +TPPRACHDECKRIC+ACTKPINGFMYHC+KDDLDLHPCCR L +NYQ
Sbjct: 61  HEFFTRSAFKFSKTPPRACHDECKRICDACTKPINGFMYHCEKDDLDLHPCCRNLNRNYQ 120

Query: 121 IEDVEFNLHKKVRGKCIWCKKRSTKSSGDDNGWSYISDCGKYHVHVACVTEMALEEWYKS 180
           IEDV FNL+K+V+GKC+WCKKR+ K  G  NGWSYIS+CG+YHVHVACVTEMALEEWYK+
Sbjct: 121 IEDVVFNLYKEVQGKCMWCKKRTIKRHGQSNGWSYISECGEYHVHVACVTEMALEEWYKN 180

Query: 181 GGDWINDNNANQDQQLALQKVNLKAIQARGRGDGGKGNKFWRILKVFIKTIVSIVLGDPT 240
           GGDW+N NNANQDQQL LQKVNLKAI+ARGRG+GGKGNK+WRILKVFIKTIVSIVLGDPT
Sbjct: 181 GGDWMNANNANQDQQLTLQKVNLKAIKARGRGNGGKGNKYWRILKVFIKTIVSIVLGDPT 240

Query: 241 TVLASLFVELIA 253
           TVLASLFVELIA
Sbjct: 241 TVLASLFVELIA 252

BLAST of Lsi02G016380.1 vs. ExPASy TrEMBL
Match: A0A5A7T4E4 (Phorbol-ester/DAG-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold311G00540 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 4.5e-130
Identity = 211/252 (83.73%), Postives = 234/252 (92.86%), Query Frame = 0

Query: 1   MAKQLCNPTYLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFPKSSPIS 60
           MAKQLC P  LKHELELKKYLKPYTCDGCKEIGFGPRYRCEKC+FDLHKACMFP++SP+ 
Sbjct: 1   MAKQLCTPNQLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCDFDLHKACMFPQNSPVF 60

Query: 61  HIFFPRSTFKFYRTPPRACHDECKRICEACTKPINGFMYHCKKDDLDLHPCCRKLKKNYQ 120
           H FFPRSTFKF +TPPRACHDECKRIC+ACTKPINGFMYHC+KD LDLHPCCR L + YQ
Sbjct: 61  HEFFPRSTFKFLKTPPRACHDECKRICDACTKPINGFMYHCEKDQLDLHPCCRNLNRKYQ 120

Query: 121 IEDVEFNLHKKVRGKCIWCKKRSTKSSGDDNGWSYISDCGKYHVHVACVTEMALEEWYKS 180
           IEDV+FNL+K+V+ KC+WCKK++ K  G  NGWSYIS+CG+YHVHVACVTEMALEEWYK+
Sbjct: 121 IEDVKFNLYKEVQRKCMWCKKKTIKRHGQSNGWSYISECGEYHVHVACVTEMALEEWYKN 180

Query: 181 GGDWINDNNANQDQQLALQKVNLKAIQARGRGDGGKGNKFWRILKVFIKTIVSIVLGDPT 240
           GG WIN NNANQD+QLALQKVNLKAI+ARGRG+GGKGNK+WRILKVFI+TIVSIVLGDPT
Sbjct: 181 GGGWINANNANQDEQLALQKVNLKAIKARGRGNGGKGNKYWRILKVFIETIVSIVLGDPT 240

Query: 241 TVLASLFVELIA 253
           TVLASLFVELIA
Sbjct: 241 TVLASLFVELIA 252

BLAST of Lsi02G016380.1 vs. ExPASy TrEMBL
Match: A0A0A0KNT1 (Phorbol-ester/DAG-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G516820 PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 4.1e-123
Identity = 202/253 (79.84%), Postives = 226/253 (89.33%), Query Frame = 0

Query: 1   MAKQLCNPTYLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFPKSSPIS 60
           M  QLC P  LKHELELK+YLKPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFP+ SPI 
Sbjct: 1   MPNQLCTPNQLKHELELKEYLKPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFPQDSPIF 60

Query: 61  HIFFPRSTFKFYRTPPRACHDECKRICEACTKPINGFMYHCK--KDDLDLHPCCRKLKKN 120
           H FFP+S F+F++TPPRAC D+CK+ C+ACTKPINGFMYHCK  K +LDLHPCCR L +N
Sbjct: 61  HEFFPQSAFEFFKTPPRACCDDCKKFCDACTKPINGFMYHCKKVKVELDLHPCCRNLNRN 120

Query: 121 YQIEDVEFNLHKKVRGKCIWCKKRSTKSSGDDNGWSYISDCGKYHVHVACVTEMALEEWY 180
           Y+IEDVEFNL+KKV+GKC+WCKKR+    G  NGWSYIS+CG+YHVHVACV EMALEEW+
Sbjct: 121 YKIEDVEFNLYKKVKGKCMWCKKRTINPHGQTNGWSYISECGEYHVHVACVIEMALEEWH 180

Query: 181 KSGGDWINDNNANQDQQLALQKVNLKAIQARGRGDGGKGNKFWRILKVFIKTIVSIVLGD 240
           K+GG+WIN NNANQDQQLALQK+NLKAI+ARGRG GGKGNK+WRILKVFI TIVSIVLGD
Sbjct: 181 KNGGNWINANNANQDQQLALQKLNLKAIKARGRGKGGKGNKYWRILKVFIGTIVSIVLGD 240

Query: 241 PTTVLASLFVELI 252
           PTTVL SLFVELI
Sbjct: 241 PTTVLVSLFVELI 253

BLAST of Lsi02G016380.1 vs. ExPASy TrEMBL
Match: A0A5A7T8P1 (Phorbol-ester/DAG-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold311G00550 PE=4 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 6.3e-116
Identity = 193/254 (75.98%), Postives = 219/254 (86.22%), Query Frame = 0

Query: 1   MAKQLCNPTYLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFPKSSPIS 60
           MA QLC P  LKH+L+LKKYLKPYTCDGCKEIGFGPRYRCE+CNFDLHKACMFP+ SP  
Sbjct: 1   MANQLCTPNKLKHKLQLKKYLKPYTCDGCKEIGFGPRYRCEECNFDLHKACMFPQDSPTF 60

Query: 61  HIFFPRSTFKFYRTPPRACHDECKRICEACTKPINGFMYHCK--KDDLDLHPCCRKLKKN 120
           H FFP+S F+F +TPP+ACHD+CK+ C+ACTK INGFMYHCK  KD+LDLHPCCR L KN
Sbjct: 61  HEFFPQSAFEFLKTPPKACHDQCKKFCDACTKTINGFMYHCKRDKDELDLHPCCRNLDKN 120

Query: 121 YQIEDVEFNLHKKVRGKCIWCKKRSTKSSGDDNGWSYISDCGKYHVHVACVTEMALEEWY 180
           Y+IE+VEFNL  KV G+C+WCKK +       NGWSYIS  GKY VHVACVTEMALEEW+
Sbjct: 121 YKIENVEFNLSNKVEGECMWCKKETINPHVKSNGWSYISKRGKYLVHVACVTEMALEEWH 180

Query: 181 KSGGDWINDNNANQDQQLALQKVNLKAIQARGRGDGGKGNKFWRILKVFIKTIVSIVLGD 240
           K+G DWIN NNANQD QLAL+KV LKAI+ARGRG+G KGNK+W+++KVFIKTIVSIVLGD
Sbjct: 181 KNGRDWINANNANQDIQLALEKVTLKAIKARGRGEGSKGNKYWKVIKVFIKTIVSIVLGD 240

Query: 241 PTTVLASLFVELIA 253
           PTTVLASLFVELIA
Sbjct: 241 PTTVLASLFVELIA 254

BLAST of Lsi02G016380.1 vs. ExPASy TrEMBL
Match: A0A1S3C8J6 (uncharacterized protein LOC103498190 OS=Cucumis melo OX=3656 GN=LOC103498190 PE=4 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 6.3e-116
Identity = 193/254 (75.98%), Postives = 219/254 (86.22%), Query Frame = 0

Query: 1   MAKQLCNPTYLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFPKSSPIS 60
           MA QLC P  LKH+L+LKKYLKPYTCDGCKEIGFGPRYRCE+CNFDLHKACMFP+ SP  
Sbjct: 1   MANQLCTPNKLKHKLQLKKYLKPYTCDGCKEIGFGPRYRCEECNFDLHKACMFPQDSPTF 60

Query: 61  HIFFPRSTFKFYRTPPRACHDECKRICEACTKPINGFMYHCK--KDDLDLHPCCRKLKKN 120
           H FFP+S F+F +TPP+ACHD+CK+ C+ACTK INGFMYHCK  KD+LDLHPCCR L KN
Sbjct: 61  HEFFPQSAFEFLKTPPKACHDQCKKFCDACTKTINGFMYHCKRDKDELDLHPCCRNLDKN 120

Query: 121 YQIEDVEFNLHKKVRGKCIWCKKRSTKSSGDDNGWSYISDCGKYHVHVACVTEMALEEWY 180
           Y+IE+VEFNL  KV G+C+WCKK +       NGWSYIS  GKY VHVACVTEMALEEW+
Sbjct: 121 YKIENVEFNLSNKVEGECMWCKKETINPHVKSNGWSYISKRGKYLVHVACVTEMALEEWH 180

Query: 181 KSGGDWINDNNANQDQQLALQKVNLKAIQARGRGDGGKGNKFWRILKVFIKTIVSIVLGD 240
           K+G DWIN NNANQD QLAL+KV LKAI+ARGRG+G KGNK+W+++KVFIKTIVSIVLGD
Sbjct: 181 KNGRDWINANNANQDIQLALEKVTLKAIKARGRGEGSKGNKYWKVIKVFIKTIVSIVLGD 240

Query: 241 PTTVLASLFVELIA 253
           PTTVLASLFVELIA
Sbjct: 241 PTTVLASLFVELIA 254

BLAST of Lsi02G016380.1 vs. NCBI nr
Match: XP_038891268.1 (uncharacterized protein LOC120080612 [Benincasa hispida])

HSP 1 Score: 480.7 bits (1236), Expect = 7.6e-132
Identity = 214/252 (84.92%), Postives = 236/252 (93.65%), Query Frame = 0

Query: 1   MAKQLCNPTYLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFPKSSPIS 60
           MA+QLCNP++LKHELELKKYLKPYTCDGCKEIGFGPRYRCEKC+FDLHKACMFPK+SPIS
Sbjct: 1   MAEQLCNPSHLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCDFDLHKACMFPKNSPIS 60

Query: 61  HIFFPRSTFKFYRTPPRACHDECKRICEACTKPINGFMYHCKKDDLDLHPCCRKLKKNYQ 120
           H FFPRSTFKF++ PPRACHD+C+RIC+ACTKPINGFMYHCKK DLDLHPCC  LK NYQ
Sbjct: 61  HEFFPRSTFKFFKIPPRACHDKCRRICDACTKPINGFMYHCKKRDLDLHPCCENLKMNYQ 120

Query: 121 IEDVEFNLHKKVRGKCIWCKKRSTKSSGDDNGWSYISDCGKYHVHVACVTEMALEEWYKS 180
           IEDVEFNLHK+V+G+C+WC K++ +S G  NGWSYIS+C KYHVHVAC TEMALEEWYK+
Sbjct: 121 IEDVEFNLHKEVQGECMWCNKKTIRSHGQHNGWSYISECRKYHVHVACATEMALEEWYKN 180

Query: 181 GGDWINDNNANQDQQLALQKVNLKAIQARGRGDGGKGNKFWRILKVFIKTIVSIVLGDPT 240
           GGDWIN +NA+QD  LALQKVNLKAIQARGRGDGGKGNKFWRILKVFIKTIVSIVLGDPT
Sbjct: 181 GGDWINVDNADQDPNLALQKVNLKAIQARGRGDGGKGNKFWRILKVFIKTIVSIVLGDPT 240

Query: 241 TVLASLFVELIA 253
           TVLASLF+ELIA
Sbjct: 241 TVLASLFIELIA 252

BLAST of Lsi02G016380.1 vs. NCBI nr
Match: XP_011656238.1 (uncharacterized protein LOC105435689 [Cucumis sativus] >KGN51307.1 hypothetical protein Csa_008103 [Cucumis sativus])

HSP 1 Score: 478.4 bits (1230), Expect = 3.8e-131
Identity = 214/252 (84.92%), Postives = 233/252 (92.46%), Query Frame = 0

Query: 1   MAKQLCNPTYLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFPKSSPIS 60
           MAKQLC P  LKHELELKKYLKPYTCDGCKEIGFGPRYRCEKC+FDLHKACMFP+ SP+ 
Sbjct: 1   MAKQLCTPNQLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCDFDLHKACMFPQDSPVF 60

Query: 61  HIFFPRSTFKFYRTPPRACHDECKRICEACTKPINGFMYHCKKDDLDLHPCCRKLKKNYQ 120
           H FF RS FKF +TPPRACHDECKRIC+ACTKPINGFMYHC+KDDLDLHPCCR L +NYQ
Sbjct: 61  HEFFTRSAFKFSKTPPRACHDECKRICDACTKPINGFMYHCEKDDLDLHPCCRNLNRNYQ 120

Query: 121 IEDVEFNLHKKVRGKCIWCKKRSTKSSGDDNGWSYISDCGKYHVHVACVTEMALEEWYKS 180
           IEDV FNL+K+V+GKC+WCKKR+ K  G  NGWSYIS+CG+YHVHVACVTEMALEEWYK+
Sbjct: 121 IEDVVFNLYKEVQGKCMWCKKRTIKRHGQSNGWSYISECGEYHVHVACVTEMALEEWYKN 180

Query: 181 GGDWINDNNANQDQQLALQKVNLKAIQARGRGDGGKGNKFWRILKVFIKTIVSIVLGDPT 240
           GGDW+N NNANQDQQL LQKVNLKAI+ARGRG+GGKGNK+WRILKVFIKTIVSIVLGDPT
Sbjct: 181 GGDWMNANNANQDQQLTLQKVNLKAIKARGRGNGGKGNKYWRILKVFIKTIVSIVLGDPT 240

Query: 241 TVLASLFVELIA 253
           TVLASLFVELIA
Sbjct: 241 TVLASLFVELIA 252

BLAST of Lsi02G016380.1 vs. NCBI nr
Match: KAA0037668.1 (uncharacterized protein E6C27_scaffold277G003360 [Cucumis melo var. makuwa] >TYK22811.1 uncharacterized protein E5676_scaffold311G00540 [Cucumis melo var. makuwa])

HSP 1 Score: 473.8 bits (1218), Expect = 9.3e-130
Identity = 211/252 (83.73%), Postives = 234/252 (92.86%), Query Frame = 0

Query: 1   MAKQLCNPTYLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFPKSSPIS 60
           MAKQLC P  LKHELELKKYLKPYTCDGCKEIGFGPRYRCEKC+FDLHKACMFP++SP+ 
Sbjct: 1   MAKQLCTPNQLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCDFDLHKACMFPQNSPVF 60

Query: 61  HIFFPRSTFKFYRTPPRACHDECKRICEACTKPINGFMYHCKKDDLDLHPCCRKLKKNYQ 120
           H FFPRSTFKF +TPPRACHDECKRIC+ACTKPINGFMYHC+KD LDLHPCCR L + YQ
Sbjct: 61  HEFFPRSTFKFLKTPPRACHDECKRICDACTKPINGFMYHCEKDQLDLHPCCRNLNRKYQ 120

Query: 121 IEDVEFNLHKKVRGKCIWCKKRSTKSSGDDNGWSYISDCGKYHVHVACVTEMALEEWYKS 180
           IEDV+FNL+K+V+ KC+WCKK++ K  G  NGWSYIS+CG+YHVHVACVTEMALEEWYK+
Sbjct: 121 IEDVKFNLYKEVQRKCMWCKKKTIKRHGQSNGWSYISECGEYHVHVACVTEMALEEWYKN 180

Query: 181 GGDWINDNNANQDQQLALQKVNLKAIQARGRGDGGKGNKFWRILKVFIKTIVSIVLGDPT 240
           GG WIN NNANQD+QLALQKVNLKAI+ARGRG+GGKGNK+WRILKVFI+TIVSIVLGDPT
Sbjct: 181 GGGWINANNANQDEQLALQKVNLKAIKARGRGNGGKGNKYWRILKVFIETIVSIVLGDPT 240

Query: 241 TVLASLFVELIA 253
           TVLASLFVELIA
Sbjct: 241 TVLASLFVELIA 252

BLAST of Lsi02G016380.1 vs. NCBI nr
Match: XP_011656237.1 (uncharacterized protein LOC105435688 [Cucumis sativus] >KGN51305.1 hypothetical protein Csa_008405 [Cucumis sativus])

HSP 1 Score: 450.7 bits (1158), Expect = 8.5e-123
Identity = 202/253 (79.84%), Postives = 226/253 (89.33%), Query Frame = 0

Query: 1   MAKQLCNPTYLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFPKSSPIS 60
           M  QLC P  LKHELELK+YLKPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFP+ SPI 
Sbjct: 1   MPNQLCTPNQLKHELELKEYLKPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFPQDSPIF 60

Query: 61  HIFFPRSTFKFYRTPPRACHDECKRICEACTKPINGFMYHCK--KDDLDLHPCCRKLKKN 120
           H FFP+S F+F++TPPRAC D+CK+ C+ACTKPINGFMYHCK  K +LDLHPCCR L +N
Sbjct: 61  HEFFPQSAFEFFKTPPRACCDDCKKFCDACTKPINGFMYHCKKVKVELDLHPCCRNLNRN 120

Query: 121 YQIEDVEFNLHKKVRGKCIWCKKRSTKSSGDDNGWSYISDCGKYHVHVACVTEMALEEWY 180
           Y+IEDVEFNL+KKV+GKC+WCKKR+    G  NGWSYIS+CG+YHVHVACV EMALEEW+
Sbjct: 121 YKIEDVEFNLYKKVKGKCMWCKKRTINPHGQTNGWSYISECGEYHVHVACVIEMALEEWH 180

Query: 181 KSGGDWINDNNANQDQQLALQKVNLKAIQARGRGDGGKGNKFWRILKVFIKTIVSIVLGD 240
           K+GG+WIN NNANQDQQLALQK+NLKAI+ARGRG GGKGNK+WRILKVFI TIVSIVLGD
Sbjct: 181 KNGGNWINANNANQDQQLALQKLNLKAIKARGRGKGGKGNKYWRILKVFIGTIVSIVLGD 240

Query: 241 PTTVLASLFVELI 252
           PTTVL SLFVELI
Sbjct: 241 PTTVLVSLFVELI 253

BLAST of Lsi02G016380.1 vs. NCBI nr
Match: XP_008458930.1 (PREDICTED: uncharacterized protein LOC103498190 [Cucumis melo] >KAA0037669.1 uncharacterized protein E6C27_scaffold277G003370 [Cucumis melo var. makuwa] >TYK22812.1 uncharacterized protein E5676_scaffold311G00550 [Cucumis melo var. makuwa])

HSP 1 Score: 426.8 bits (1096), Expect = 1.3e-115
Identity = 193/254 (75.98%), Postives = 219/254 (86.22%), Query Frame = 0

Query: 1   MAKQLCNPTYLKHELELKKYLKPYTCDGCKEIGFGPRYRCEKCNFDLHKACMFPKSSPIS 60
           MA QLC P  LKH+L+LKKYLKPYTCDGCKEIGFGPRYRCE+CNFDLHKACMFP+ SP  
Sbjct: 1   MANQLCTPNKLKHKLQLKKYLKPYTCDGCKEIGFGPRYRCEECNFDLHKACMFPQDSPTF 60

Query: 61  HIFFPRSTFKFYRTPPRACHDECKRICEACTKPINGFMYHCK--KDDLDLHPCCRKLKKN 120
           H FFP+S F+F +TPP+ACHD+CK+ C+ACTK INGFMYHCK  KD+LDLHPCCR L KN
Sbjct: 61  HEFFPQSAFEFLKTPPKACHDQCKKFCDACTKTINGFMYHCKRDKDELDLHPCCRNLDKN 120

Query: 121 YQIEDVEFNLHKKVRGKCIWCKKRSTKSSGDDNGWSYISDCGKYHVHVACVTEMALEEWY 180
           Y+IE+VEFNL  KV G+C+WCKK +       NGWSYIS  GKY VHVACVTEMALEEW+
Sbjct: 121 YKIENVEFNLSNKVEGECMWCKKETINPHVKSNGWSYISKRGKYLVHVACVTEMALEEWH 180

Query: 181 KSGGDWINDNNANQDQQLALQKVNLKAIQARGRGDGGKGNKFWRILKVFIKTIVSIVLGD 240
           K+G DWIN NNANQD QLAL+KV LKAI+ARGRG+G KGNK+W+++KVFIKTIVSIVLGD
Sbjct: 181 KNGRDWINANNANQDIQLALEKVTLKAIKARGRGEGSKGNKYWKVIKVFIKTIVSIVLGD 240

Query: 241 PTTVLASLFVELIA 253
           PTTVLASLFVELIA
Sbjct: 241 PTTVLASLFVELIA 254

BLAST of Lsi02G016380.1 vs. TAIR 10
Match: AT2G16050.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 162.2 bits (409), Expect = 5.6e-40
Identity = 89/246 (36.18%), Postives = 127/246 (51.63%), Query Frame = 0

Query: 12  KHELELKKYLKPYTCDGCKEIGFGPRYRCE-----KCNFDLHKACMFPKSSPISHIFFPR 71
           +H L+     KP+ CDGC E+G G RYRC       C+FDLH  C  P +S I+H F+ +
Sbjct: 13  QHNLKFDYTEKPFKCDGCNEVGIGSRYRCSGDNHGSCDFDLHAHCALPSAS-ITHPFYKK 72

Query: 72  STFKFYRTPPRACHDECKRICEACTKPINGFMYHCKKDDLDLHPCCRKLKKNYQIEDVEF 131
            TF+F   PP       +R C AC K ++GF+YHCK    DLHPCC  L       +   
Sbjct: 73  CTFQFVARPP----GNERRYCNACEKDVSGFLYHCKACGFDLHPCCAMLPMVLDDGETNL 132

Query: 132 NLHKKVRGKCIWCKKRSTKSSGDDNGWSYISDCGKYHVHVACVTEMALEEWYKSGGDWIN 191
            L++KV   C  C K+          WSY S C KY++HVACV EM +E W +    +  
Sbjct: 133 FLYRKVSSSCHRCGKKG-------RSWSYRSSCKKYNLHVACVREMLVENWREL---YTG 192

Query: 192 DNNANQDQQLALQKVNLKAIQARGRGDGGKGNKFWRILKVFIKTIVSIVLGDPTTVLASL 251
            +  + + +  + K  L+      R   GK  K   I  + ++ ++S VLGDPTT++A +
Sbjct: 193 QHGKSIEHKSLVLKNTLEHHHRSSR--KGKVQKCCEIAGIAVQFVISAVLGDPTTLIAGV 241

Query: 252 FVELIA 253
              LI+
Sbjct: 253 VGSLIS 241

BLAST of Lsi02G016380.1 vs. TAIR 10
Match: AT2G37800.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 77.8 bits (190), Expect = 1.4e-14
Identity = 63/251 (25.10%), Postives = 98/251 (39.04%), Query Frame = 0

Query: 24  YTCDGCKEIGFGPRYRCEKCNFDLHKACMFPKSSPISHIFFPRSTFKFYRTPPRACHDEC 83
           +TCDGCK  GFG  YRC +C+++LH  C    S+ ++    P+   +     P   H   
Sbjct: 163 FTCDGCKTYGFGKTYRCTRCDYNLHDHCATCPST-LATFMHPQHELRLVFRGPEHTHQN- 222

Query: 84  KRICEACTKPINGFMYHCKKDDLDLHPCCRKLKKNYQIEDVEFNLHKKVRGK------CI 143
           KR+C+ C +   G  Y C+    D+HP C +L ++  +  V    H     +      C+
Sbjct: 223 KRMCDICDESAEGLYYQCEPCGFDVHPLCTQLPQH--VRHVPHPAHLLELSQWGASSICM 282

Query: 144 WCKKRSTKSSGDDNGWSY-ISDCGKYHVHVACVTEMAL--------------------EE 203
            C+       G    W Y    CG   VH+ C++  A                      E
Sbjct: 283 VCR-------GAIRSWRYKCGPCG-LDVHMECISSSASVAATKIQQRCFGSPQPQFYHSE 342

Query: 204 WYKSGGDWINDNNANQDQQLALQKVNLKAIQARGRGDGGKGNKFWRILKVFIKTIVSIVL 248
           +Y     + N    NQ Q       N   +Q  GR  G K   F  ++ + +  + +I+ 
Sbjct: 343 YYYHHA-YYNHGYTNQGQVHGY--ANQGQVQEPGRSKGRK--MFSILMDLTVGVVCNIIA 396

BLAST of Lsi02G016380.1 vs. TAIR 10
Match: AT1G20990.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 75.5 bits (184), Expect = 6.9e-14
Identity = 44/165 (26.67%), Postives = 69/165 (41.82%), Query Frame = 0

Query: 24  YTCDGCKEIGFGPRYRCEKCNFDLHKACMFPKSSPISHIFFPRSTFKFYRTPPRACHDEC 83
           YTC GCKE G G RY C++C++ LH+ C        SH F  +    F+  P +      
Sbjct: 87  YTCAGCKEEGAGVRYVCQECDYQLHEFCALAPPQLKSHPFHYQHQLLFFAKPAKG--GIV 146

Query: 84  KRICEACTKPINGFMYHCKKDDLDLHPCCRKLKKNYQIEDVEFNLHKKVRGKCIWCKKRS 143
           K  C+ C +   G+ + CK     +HP C  L  +     +    H  +R   +     +
Sbjct: 147 KSKCDVCGRSPKGYTFRCKACSFQMHPGCAMLSPSLSSSSLH---HHPLR---LLPSSSA 206

Query: 144 TKSSGDDNGWSYISDC--GK------------YHVHVACVTEMAL 175
             ++G D+G     +C  GK            YH+H  C  + A+
Sbjct: 207 GSTTGGDSGGFLCGECKRGKRTGRVYRCTVCDYHLHAVCAKDAAV 243

BLAST of Lsi02G016380.1 vs. TAIR 10
Match: AT2G37820.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 75.5 bits (184), Expect = 6.9e-14
Identity = 43/163 (26.38%), Postives = 68/163 (41.72%), Query Frame = 0

Query: 13  HELELKKYLKPYTCDGCKEIGFGPRYRCEKCNFDLHKAC-MFPKSSPISHIFFPRSTFKF 72
           H L     +  + CDGCK  G G  YRCE CN+DLH+ C   P + P      P+     
Sbjct: 18  HPLTEFNSIGDFICDGCKTYGSGKTYRCEPCNYDLHEYCATCPLTLPT--FIHPQHELSL 77

Query: 73  YRTPPRACHDECKRICEACTKPINGFMYHCKKDDLDLHPCCRKLKKN-----YQIEDVEF 132
                ++      R C+ C + + G  Y CK  + D+HP C +L ++     +    +EF
Sbjct: 78  VVRKQQSTRQN-DRACDICDESVEGLFYRCKICEFDVHPLCTQLPQHVRHVLHPAHHLEF 137

Query: 133 NLHKKVRGKCIWCKKRSTKSSGDDNGWSYISDCGKYHVHVACV 170
                    C+ C        G    W Y  +  ++ +H+ C+
Sbjct: 138 --RPSGASTCMVC-------HGPCQSWRYRCELCRFDIHMECI 168

BLAST of Lsi02G016380.1 vs. TAIR 10
Match: AT2G37810.1 (Cysteine/Histidine-rich C1 domain family protein )

HSP 1 Score: 65.9 bits (159), Expect = 5.5e-11
Identity = 41/150 (27.33%), Postives = 58/150 (38.67%), Query Frame = 0

Query: 24  YTCDGCKEIGFGPRYRCEKCNFDLHKACMFPKSSPISHIFFPRSTFKFYRTPPRACHDEC 83
           +TC GCK  GFG  YRC  C++ LH  C    + P + I F     +      R   +  
Sbjct: 29  FTCGGCKTYGFGKTYRCAPCDYVLHDHC---ATCPFTLISFMHPQHEL-----RLFVNGS 88

Query: 84  KRICEACTKPINGFMYHCKKDDLDLHPCCRKLKKNYQIEDVEFNLHKKVRGKCIWCKKRS 143
           + +C  C + + G  YHC+    D+HP C   + +  +  V    H     +C       
Sbjct: 89  ENMCNICHRLVEGVYYHCETCGFDVHPLC--TQHHQHVSYVPHPAHLLELSQC-GASNTC 148

Query: 144 TKSSGDDNGWSYISDCGKYHVHVACVTEMA 174
               G    W Y        VHV CV   A
Sbjct: 149 MVCRGAILSWRYKCGPCMLDVHVECVNSSA 167

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7Y0E87.5e-0545.28Probable nucleoredoxin 1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405... [more]
Q7Y0F21.7e-0437.50Probable nucleoredoxin 1-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405... [more]
Match NameE-valueIdentityDescription
A0A0A0KS981.8e-13184.92Phorbol-ester/DAG-type domain-containing protein OS=Cucumis sativus OX=3659 GN=C... [more]
A0A5A7T4E44.5e-13083.73Phorbol-ester/DAG-type domain-containing protein OS=Cucumis melo var. makuwa OX=... [more]
A0A0A0KNT14.1e-12379.84Phorbol-ester/DAG-type domain-containing protein OS=Cucumis sativus OX=3659 GN=C... [more]
A0A5A7T8P16.3e-11675.98Phorbol-ester/DAG-type domain-containing protein OS=Cucumis melo var. makuwa OX=... [more]
A0A1S3C8J66.3e-11675.98uncharacterized protein LOC103498190 OS=Cucumis melo OX=3656 GN=LOC103498190 PE=... [more]
Match NameE-valueIdentityDescription
XP_038891268.17.6e-13284.92uncharacterized protein LOC120080612 [Benincasa hispida][more]
XP_011656238.13.8e-13184.92uncharacterized protein LOC105435689 [Cucumis sativus] >KGN51307.1 hypothetical ... [more]
KAA0037668.19.3e-13083.73uncharacterized protein E6C27_scaffold277G003360 [Cucumis melo var. makuwa] >TYK... [more]
XP_011656237.18.5e-12379.84uncharacterized protein LOC105435688 [Cucumis sativus] >KGN51305.1 hypothetical ... [more]
XP_008458930.11.3e-11575.98PREDICTED: uncharacterized protein LOC103498190 [Cucumis melo] >KAA0037669.1 unc... [more]
Match NameE-valueIdentityDescription
AT2G16050.15.6e-4036.18Cysteine/Histidine-rich C1 domain family protein [more]
AT2G37800.11.4e-1425.10Cysteine/Histidine-rich C1 domain family protein [more]
AT1G20990.16.9e-1426.67Cysteine/Histidine-rich C1 domain family protein [more]
AT2G37820.16.9e-1426.38Cysteine/Histidine-rich C1 domain family protein [more]
AT2G37810.15.5e-1127.33Cysteine/Histidine-rich C1 domain family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004146DC1PFAMPF03107C1_2coord: 12..52
e-value: 3.8E-9
score: 36.7
NoneNo IPR availableGENE3D3.30.60.20coord: 11..56
e-value: 3.6E-8
score: 35.0
NoneNo IPR availablePANTHERPTHR46477:SF5SUBFAMILY NOT NAMEDcoord: 8..251
NoneNo IPR availablePANTHERPTHR46477CYSTEINE/HISTIDINE-RICH C1 DOMAIN FAMILY PROTEINcoord: 8..251
NoneNo IPR availableSUPERFAMILY57889Cysteine-rich domaincoord: 59..119
NoneNo IPR availableSUPERFAMILY57889Cysteine-rich domaincoord: 7..53
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainPROSITEPS50081ZF_DAG_PE_2coord: 12..51
score: 8.553101

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi02G016380Lsi02G016380gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi02G016380.1.exon.1Lsi02G016380.1.exon.1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi02G016380.1.CDS.1Lsi02G016380.1.CDS.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi02G016380.1Lsi02G016380.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
cellular_component GO:0016021 integral component of membrane