Lsi02G008240.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G008240.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Rho_N domain-containing protein
Location	chr02: 7680936 .. 7682391 (-)
Sequence length	1071
RNA-Seq Expression	Lsi02G008240.1
Synteny	Lsi02G008240.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCAATAGTTTTCCAGCCTCGGATTCTAATCCGCTTTCCCAATTTGGTTTCTTTGGGAAGGAGACCCACGTTCGCTTCGAAAGGTATCAAGCTTGAAACTCCCAAGTCCCAAGGAACCAGTCTTGAATTTCGCAAATCTATTGTTTGTCCATTTCATTCAGCTTTGTCTGCTTAGCTAACTATTCAAAGTGAACAATCTATTTGAGTTTTGTCATCACTTTTTATTGCAGAATTTGTAGATCTTGTGTACTTCACAAGCTGAGGGATTGATTTTTGATGCAGCTTGTTAAATTTGGCAGACATTGCAGATGTTTATCCCAGTAAGAGCATTCAACTATCTGTTACAAACAATTGGCCAGATGGAAATGCAGGGAATCGGCCTCCTCGTAGAATCTCTGCGCCAGGAAAAAGGAGGAAGAATGAACCTTCTTCGAGGAAAACAGAAGCCCACAAGGAGGAAGACCTAAAAAAACCAAAATTCAATAACCAGGAGGAAATAATTGCTCTGTTCAGAAAGATTCAGACTTCCATTGCTAAAGAATCCGCAACCTCTGATGATGAATCCTGCAAGGATGAACAACAAGGAGCCGATTCTATTTTAGAGACTCTTCGTGAATCAAGGAAGCAAGTGAAAGGTAATTTCTTGATTGTCTCGTCTGACGCTCTTGTGGGAAATTTTGTCCACCTTTAAATCTATGAATGCATCTCGCAAGAACGAAAATTTATAGTTTATAAACATCTCGCAAGAAACAAGTGACCACTATTAATTGGCAATATGATTCCATTCCCGAACCAACATACATATCAAGTACATTTAGCAATATCATTGTCAATATACATTGTCACAAGGTATGATTGAAAAAATTTCGATCTTATCTTTGCTAAATAATAGAGTATGGACAGGCAAAACTTCTAAGAAGGCAGGAGCTAAAGTGTTGAGAAGTAAAGGCACGTCTGAAGAGAAGGAAATGCAGGATCCTTCACCACCAGCTGCAGATTTCCAGTTGGTTCGACCACTCTCTAAATTTGTGAAGAGATCACCAATTCCGTCTCCCCCACGAGGCCGTGGTTCACACCTTAGAGTTGAGGCATCTCAGGCCATAGCTGAAAGCAGGGAGTTGAAGTTCACAAGTATAGAGAATATGAAACTTACCGAGCTGAAAGCACTAGCAAAATCTAGAGGAATTAAGGGTTACTCCAAATTGAAGAAAAATGAGCTCATGGAAGTCCTGGGATCCTAAATCATGTCAGGGCATTTTGAGGAGAAATCATCGGTCTCTTCTATTTTTATTTTTTTACTTTTCCTTTGGTGTTCCTCTTTCTACTTGCTTCACATGTTTTGATAACAAAATTGCAAGCTGGTCTTCCAGTGGATCGTAACATTCAAAAACCTTTGTTCATACATTAAATCTATAACGATGTATCTAGAATTTAATTCTCGAAAATTATTC

mRNA sequence

ATGGAAGCAATAGTTTTCCAGCCTCGGATTCTAATCCGCTTTCCCAATTTGGTTTCTTTGGGAAGGAGACCCACGTTCGCTTCGAAAGGTATCAAGCTTGAAACTCCCAAGTCCCAAGGAACCAGTCTTGAATTTCGCAAATCTATTGTTTGTCCATTTCATTCAGCTTTCTTGTTAAATTTGGCAGACATTGCAGATGTTTATCCCAGTAAGAGCATTCAACTATCTGTTACAAACAATTGGCCAGATGGAAATGCAGGGAATCGGCCTCCTCGTAGAATCTCTGCGCCAGGAAAAAGGAGGAAGAATGAACCTTCTTCGAGGAAAACAGAAGCCCACAAGGAGGAAGACCTAAAAAAACCAAAATTCAATAACCAGGAGGAAATAATTGCTCTGTTCAGAAAGATTCAGACTTCCATTGCTAAAGAATCCGCAACCTCTGATGATGAATCCTGCAAGGATGAACAACAAGGAGCCGATTCTATTTTAGAGACTCTTCGTGAATCAAGGAAGCAAGTGAAAGGCAAAACTTCTAAGAAGGCAGGAGCTAAAGTGTTGAGAAGTAAAGGCACGTCTGAAGAGAAGGAAATGCAGGATCCTTCACCACCAGCTGCAGATTTCCAGTTGGTTCGACCACTCTCTAAATTTGTGAAGAGATCACCAATTCCGTCTCCCCCACGAGGCCGTGGTTCACACCTTAGAGTTGAGGCATCTCAGGCCATAGCTGAAAGCAGGGAGTTGAAGTTCACAAGTATAGAGAATATGAAACTTACCGAGCTGAAAGCACTAGCAAAATCTAGAGGAATTAAGGGTTACTCCAAATTGAAGAAAAATGAGCTCATGGAAGTCCTGGGATCCTAAATCATGTCAGGGCATTTTGAGGAGAAATCATCGGTCTCTTCTATTTTTATTTTTTTACTTTTCCTTTGGTGTTCCTCTTTCTACTTGCTTCACATGTTTTGATAACAAAATTGCAAGCTGGTCTTCCAGTGGATCGTAACATTCAAAAACCTTTGTTCATACATTAAATCTATAACGATGTATCTAGAATTTAATTCTCGAAAATTATTC

Coding sequence (CDS)

ATGGAAGCAATAGTTTTCCAGCCTCGGATTCTAATCCGCTTTCCCAATTTGGTTTCTTTGGGAAGGAGACCCACGTTCGCTTCGAAAGGTATCAAGCTTGAAACTCCCAAGTCCCAAGGAACCAGTCTTGAATTTCGCAAATCTATTGTTTGTCCATTTCATTCAGCTTTCTTGTTAAATTTGGCAGACATTGCAGATGTTTATCCCAGTAAGAGCATTCAACTATCTGTTACAAACAATTGGCCAGATGGAAATGCAGGGAATCGGCCTCCTCGTAGAATCTCTGCGCCAGGAAAAAGGAGGAAGAATGAACCTTCTTCGAGGAAAACAGAAGCCCACAAGGAGGAAGACCTAAAAAAACCAAAATTCAATAACCAGGAGGAAATAATTGCTCTGTTCAGAAAGATTCAGACTTCCATTGCTAAAGAATCCGCAACCTCTGATGATGAATCCTGCAAGGATGAACAACAAGGAGCCGATTCTATTTTAGAGACTCTTCGTGAATCAAGGAAGCAAGTGAAAGGCAAAACTTCTAAGAAGGCAGGAGCTAAAGTGTTGAGAAGTAAAGGCACGTCTGAAGAGAAGGAAATGCAGGATCCTTCACCACCAGCTGCAGATTTCCAGTTGGTTCGACCACTCTCTAAATTTGTGAAGAGATCACCAATTCCGTCTCCCCCACGAGGCCGTGGTTCACACCTTAGAGTTGAGGCATCTCAGGCCATAGCTGAAAGCAGGGAGTTGAAGTTCACAAGTATAGAGAATATGAAACTTACCGAGCTGAAAGCACTAGCAAAATCTAGAGGAATTAAGGGTTACTCCAAATTGAAGAAAAATGAGCTCATGGAAGTCCTGGGATCCTAA

Protein sequence

MEAIVFQPRILIRFPNLVSLGRRPTFASKGIKLETPKSQGTSLEFRKSIVCPFHSAFLLNLADIADVYPSKSIQLSVTNNWPDGNAGNRPPRRISAPGKRRKNEPSSRKTEAHKEEDLKKPKFNNQEEIIALFRKIQTSIAKESATSDDESCKDEQQGADSILETLRESRKQVKGKTSKKAGAKVLRSKGTSEEKEMQDPSPPAADFQLVRPLSKFVKRSPIPSPPRGRGSHLRVEASQAIAESRELKFTSIENMKLTELKALAKSRGIKGYSKLKKNELMEVLGS

Homology

[Close]

BLAST of Lsi02G008240.1 vs. ExPASy Swiss-Prot
Match: Q94K75 (Rho-N domain-containing protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RHON1 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 3.2e-04
Identity = 24/51 (47.06%), Postives = 35/51 (68.63%), Query Frame = 0


Query: 236 EASQAIAESRELKFTSIENMKLTELKALAKSRGIKGYSKLKKNELMEVLGS 287
           +A +   E+ E     +  +KL EL+ +AKSRG+KG SK+KK EL+E+LGS
Sbjct: 348 DADEDSDEAEEEAVKDLSELKLVELRGIAKSRGLKGLSKMKKAELVELLGS 398

[Close]

BLAST of Lsi02G008240.1 vs. ExPASy TrEMBL
Match: A0A0A0LX66 (Rho_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G470300 PE=4 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 4.3e-84
Identity = 200/289 (69.20%), Postives = 218/289 (75.43%), Query Frame = 0


Query: 1   MEAIVFQPRILIRFPNLVSLGRRPTFASKGIKLETPKSQGTSLEFRKSIVCPFHSAFLLN 60
           MEA+VF PRILIRFPNL+SL RRPTFASK                               
Sbjct: 1   MEAVVFLPRILIRFPNLISLRRRPTFASK------------------------------- 60

Query: 61  LADIADVYPSKSIQLSVTNNWPDGNAGNRPPRRISAPGKRRKNEPSSRKTEAHK-EEDLK 120
             D+ADVYPSK+IQ SV+ + PDGNAGNRPPRR S PGK RK+E SSRKTE  K EE +K
Sbjct: 61  --DVADVYPSKNIQPSVSKSRPDGNAGNRPPRRNSLPGKARKSESSSRKTETPKDEESVK 120

Query: 121 KPKFNNQEEIIALFRKIQTSIAKESATS-DDESCKDEQQGADSILETLRESRKQVKGKTS 180
           K + N+QEE+IALFRKIQTSIAKESA+S D+ES KDE     SILETLRESRKQ+KGKTS
Sbjct: 121 KSETNDQEEVIALFRKIQTSIAKESASSIDEESRKDENA---SILETLRESRKQLKGKTS 180

Query: 181 KKAGAKVLRSKGTSEEKEMQDPS-PPAADFQLVRPLSKFVKRSPIPSPPRGRGSHLRVEA 240
           KKAGAKVLRSKG SEEKEM DPS PPAADF+LVRP SKFVKRSPIP         L+V+A
Sbjct: 181 KKAGAKVLRSKGKSEEKEMHDPSPPPAADFKLVRPPSKFVKRSPIP---------LKVDA 240

Query: 241 SQAIAESRELKFTSIENMKLTELKALAKSRGIKGYSKLKKNELMEVLGS 287
           SQAIAESRELKF S ENMKLTELKALAKSRGIKGYSKLKKNELME+L S
Sbjct: 241 SQAIAESRELKFPSTENMKLTELKALAKSRGIKGYSKLKKNELMEILRS 244

[Close]

BLAST of Lsi02G008240.1 vs. ExPASy TrEMBL
Match: A0A5A7UMB7 (SAP-like protein BP-73 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G002410 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 9.2e-79
Identity = 184/244 (75.41%), Postives = 198/244 (81.15%), Query Frame = 0


Query: 45  FRKSIVCPFHSAFLLNLADIADVYPSKSIQLSVTNNWPDGNAGNRPPRRISAPGKRRKNE 104
           F K     F S  LLNLADIADVYPSK+IQLSV+NN PDGNA NRPPRR S PGK RK+E
Sbjct: 42  FEKKTHVRFES--LLNLADIADVYPSKNIQLSVSNNRPDGNARNRPPRRNSLPGKTRKSE 101

Query: 105 PSSRKTEAHK-EEDLKKPKFNNQEEIIALFRKIQTSIAKESATSDDESCKDEQQGADSIL 164
            SSRK EA K EE++KK K N+QEEIIALFRKIQ SIAKESA+S DE    ++ GA SIL
Sbjct: 102 SSSRKKEAPKNEENIKKSKPNDQEEIIALFRKIQASIAKESASSIDEESHKDEHGATSIL 161

Query: 165 ETLRESRKQVKGKTSKKAGAKVLRSKGTSEEKEMQDPS-PPAADFQLVRPLSKFVKRSPI 224
           ETLRE RKQ+KGKTSKKAGAKV RSKGTSEEKEM DPS PPAADF+LVRP SKFVKRSPI
Sbjct: 162 ETLREPRKQLKGKTSKKAGAKVFRSKGTSEEKEMHDPSPPPAADFKLVRPPSKFVKRSPI 221

Query: 225 PSPPRGRGSHLRVEASQAIAESRELKFTSIENMKLTELKALAKSRGIKGYSKLKKNELME 284
           P          +V+ASQAIAESRELKF SIENMKL ELKALAKSRGIKGYSKLKKNELME
Sbjct: 222 PP---------KVDASQAIAESRELKFPSIENMKLAELKALAKSRGIKGYSKLKKNELME 274

Query: 285 VLGS 287
           +L S
Sbjct: 282 ILRS 274

[Close]

BLAST of Lsi02G008240.1 vs. ExPASy TrEMBL
Match: A0A1S3BFQ9 (SAP-like protein BP-73 OS=Cucumis melo OX=3656 GN=LOC103489342 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 9.2e-79
Identity = 184/244 (75.41%), Postives = 198/244 (81.15%), Query Frame = 0


Query: 45  FRKSIVCPFHSAFLLNLADIADVYPSKSIQLSVTNNWPDGNAGNRPPRRISAPGKRRKNE 104
           F K     F S  LLNLADIADVYPSK+IQLSV+NN PDGNA NRPPRR S PGK RK+E
Sbjct: 42  FEKKTHVRFES--LLNLADIADVYPSKNIQLSVSNNRPDGNARNRPPRRNSLPGKTRKSE 101

Query: 105 PSSRKTEAHK-EEDLKKPKFNNQEEIIALFRKIQTSIAKESATSDDESCKDEQQGADSIL 164
            SSRK EA K EE++KK K N+QEEIIALFRKIQ SIAKESA+S DE    ++ GA SIL
Sbjct: 102 SSSRKKEAPKNEENIKKSKPNDQEEIIALFRKIQASIAKESASSIDEESHKDEHGATSIL 161

Query: 165 ETLRESRKQVKGKTSKKAGAKVLRSKGTSEEKEMQDPS-PPAADFQLVRPLSKFVKRSPI 224
           ETLRE RKQ+KGKTSKKAGAKV RSKGTSEEKEM DPS PPAADF+LVRP SKFVKRSPI
Sbjct: 162 ETLREPRKQLKGKTSKKAGAKVFRSKGTSEEKEMHDPSPPPAADFKLVRPPSKFVKRSPI 221

Query: 225 PSPPRGRGSHLRVEASQAIAESRELKFTSIENMKLTELKALAKSRGIKGYSKLKKNELME 284
           P          +V+ASQAIAESRELKF SIENMKL ELKALAKSRGIKGYSKLKKNELME
Sbjct: 222 PP---------KVDASQAIAESRELKFPSIENMKLAELKALAKSRGIKGYSKLKKNELME 274

Query: 285 VLGS 287
           +L S
Sbjct: 282 ILRS 274

[Close]

BLAST of Lsi02G008240.1 vs. ExPASy TrEMBL
Match: A0A6J1CGN3 (uncharacterized protein LOC111010657 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010657 PE=4 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 4.0e-74
Identity = 184/288 (63.89%), Postives = 206/288 (71.53%), Query Frame = 0


Query: 1   MEAIVFQPRILIRFPNLVSLGRRPTFASKGIKLETPKSQGTSLEFRKSIVCPFHSAFLLN 60
           MEA+VFQ R L RFPNLVS GRRP FA                                 
Sbjct: 1   MEAVVFQSRTL-RFPNLVSFGRRPIFA--------------------------------- 60

Query: 61  LADIADVYPSKSIQLSVTNNWPDGNAGNRPPRRISAPGKRRKNEPSSRKTEAHKEEDLKK 120
           L +IA V  SK IQ+SVT+N   GNAG RPPRR S PG+ RKNEP+  + EA   EDLK 
Sbjct: 61  LKEIAAVNSSKGIQVSVTSN-GGGNAGQRPPRRSSGPGRTRKNEPN--RNEA--AEDLKN 120

Query: 121 PKFNNQEEIIALFRKIQTSIAKESATSDDESCKDEQQGADSILETLRESRKQVKGKTSKK 180
           PK NNQEEIIALFRKIQTSIAK+SAT+ DE   +++ GA+SILE+LRESRKQVKG+TSKK
Sbjct: 121 PKSNNQEEIIALFRKIQTSIAKDSATTKDEDSHEDEIGAESILESLRESRKQVKGRTSKK 180

Query: 181 AGAKVLRSKGTSEEKEMQDPSPPAADFQLVRPLSKFVKRSPIPSPP--RGRGSHLRVEAS 240
           AG KVLR KG SEE EM   S PAA+F+LVRP SKFVKRSPIPSPP   G  S LR E S
Sbjct: 181 AGVKVLRRKGISEEPEMYHTS-PAAEFKLVRPPSKFVKRSPIPSPPGRNGSRSQLREEPS 240

Query: 241 QAIAESRELKFTSIENMKLTELKALAKSRGIKGYSKLKKNELMEVLGS 287
           QAIAESRE+KF S+ENMKLTELKA+AKSRGIKGYSKLKKNEL+E+L S
Sbjct: 241 QAIAESREMKFPSVENMKLTELKAVAKSRGIKGYSKLKKNELLELLRS 248

[Close]

BLAST of Lsi02G008240.1 vs. ExPASy TrEMBL
Match: A0A6J1CF22 (uncharacterized protein LOC111010657 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010657 PE=4 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 2.7e-70
Identity = 168/236 (71.19%), Postives = 189/236 (80.08%), Query Frame = 0


Query: 53  FHSAFLLNLADIADVYPSKSIQLSVTNNWPDGNAGNRPPRRISAPGKRRKNEPSSRKTEA 112
           F    LLNLA+IA V  SK IQ+SVT+N   GNAG RPPRR S PG+ RKNEP+  + EA
Sbjct: 40  FRFESLLNLAEIAAVNSSKGIQVSVTSN-GGGNAGQRPPRRSSGPGRTRKNEPN--RNEA 99

Query: 113 HKEEDLKKPKFNNQEEIIALFRKIQTSIAKESATSDDESCKDEQQGADSILETLRESRKQ 172
              EDLK PK NNQEEIIALFRKIQTSIAK+SAT+ DE   +++ GA+SILE+LRESRKQ
Sbjct: 100 --AEDLKNPKSNNQEEIIALFRKIQTSIAKDSATTKDEDSHEDEIGAESILESLRESRKQ 159

Query: 173 VKGKTSKKAGAKVLRSKGTSEEKEMQDPSPPAADFQLVRPLSKFVKRSPIPSPP--RGRG 232
           VKG+TSKKAG KVLR KG SEE EM   S PAA+F+LVRP SKFVKRSPIPSPP   G  
Sbjct: 160 VKGRTSKKAGVKVLRRKGISEEPEMYHTS-PAAEFKLVRPPSKFVKRSPIPSPPGRNGSR 219

Query: 233 SHLRVEASQAIAESRELKFTSIENMKLTELKALAKSRGIKGYSKLKKNELMEVLGS 287
           S LR E SQAIAESRE+KF S+ENMKLTELKA+AKSRGIKGYSKLKKNEL+E+L S
Sbjct: 220 SQLREEPSQAIAESREMKFPSVENMKLTELKAVAKSRGIKGYSKLKKNELLELLRS 269

[Close]

BLAST of Lsi02G008240.1 vs. NCBI nr
Match: XP_038893911.1 (SAP-like protein BP-73 isoform X2 [Benincasa hispida])

HSP 1 Score: 407.1 bits (1045), Expect = 1.2e-109
Identity = 233/290 (80.34%), Postives = 251/290 (86.55%), Query Frame = 0


Query: 1   MEAIVFQPRILIRFPNLVSLGRRPTFASKGIKLETPKSQGTSLEFRKSIVCPFHSAF--L 60
           MEA++FQPRILIRFP LVSLGRRPTFASKGI+ E              IV  F +    L
Sbjct: 1   MEAVIFQPRILIRFPKLVSLGRRPTFASKGIEPE--------------IVLNFANPLFNL 60

Query: 61  LNLADIADVYPSKSIQLSVTNNWPDGNAGNRPPRRISAPGKRRKNEPSSRKTEAH-KEED 120
           LNLADIADVYPSK IQLSV+NN PDG AGNRPPRRISAPGK RKNEPSSRKTEAH  EED
Sbjct: 61  LNLADIADVYPSKGIQLSVSNNRPDGKAGNRPPRRISAPGKTRKNEPSSRKTEAHMNEED 120

Query: 121 LKKPKFNNQEEIIALFRKIQTSIAKESATS-DDESCKDEQQGADSILETLRESRKQVKGK 180
           LKK K+NNQEEIIALFRKI+TSIAKESA+S D+ESCKDE  GA+SILETLRESRKQVKGK
Sbjct: 121 LKKSKYNNQEEIIALFRKIETSIAKESASSNDEESCKDE-HGAESILETLRESRKQVKGK 180

Query: 181 TSKKAGAKVLRSKGTSEEKEMQDPSPPAADFQLVRPLSKFVKRSPIPSPPRGRGSHLRVE 240
           +SKKAGAK LRS+GTSEEKE+ DPSPPAADFQLVRP SKFVKRSPIPSPPRG GSH RV+
Sbjct: 181 SSKKAGAKALRSEGTSEEKEIHDPSPPAADFQLVRPPSKFVKRSPIPSPPRGNGSHPRVD 240

Query: 241 ASQAIAESRELKFTSIENMKLTELKALAKSRGIKGYSKLKKNELMEVLGS 287
           A+QAIAESRELKF SI+NMKLTELKALAKSRGIKGYSKLKKNEL+E+LGS
Sbjct: 241 ATQAIAESRELKFPSIKNMKLTELKALAKSRGIKGYSKLKKNELIELLGS 275

[Close]

BLAST of Lsi02G008240.1 vs. NCBI nr
Match: XP_038893910.1 (SAP-like protein BP-73 isoform X1 [Benincasa hispida])

HSP 1 Score: 401.4 bits (1030), Expect = 6.7e-108
Identity = 233/294 (79.25%), Postives = 251/294 (85.37%), Query Frame = 0


Query: 1   MEAIVFQPRILIRFPNLVSLGRRPTFASKGIKLETPKSQGTSLEFRKSIVCPFHSAF--L 60
           MEA++FQPRILIRFP LVSLGRRPTFASKGI+ E              IV  F +    L
Sbjct: 1   MEAVIFQPRILIRFPKLVSLGRRPTFASKGIEPE--------------IVLNFANPLFNL 60

Query: 61  LNLADIADVYPSKSIQLSVTNNWPDGNAGNRPPRRISAPGKRRKNEPSSRKTEAH-KEED 120
           LNLADIADVYPSK IQLSV+NN PDG AGNRPPRRISAPGK RKNEPSSRKTEAH  EED
Sbjct: 61  LNLADIADVYPSKGIQLSVSNNRPDGKAGNRPPRRISAPGKTRKNEPSSRKTEAHMNEED 120

Query: 121 LKKPKFNNQEEIIALFRKIQTSIAKESATS-DDESCKDEQQGADSILETLRESRKQVK-- 180
           LKK K+NNQEEIIALFRKI+TSIAKESA+S D+ESCKDE  GA+SILETLRESRKQVK  
Sbjct: 121 LKKSKYNNQEEIIALFRKIETSIAKESASSNDEESCKDE-HGAESILETLRESRKQVKEL 180

Query: 181 --GKTSKKAGAKVLRSKGTSEEKEMQDPSPPAADFQLVRPLSKFVKRSPIPSPPRGRGSH 240
             GK+SKKAGAK LRS+GTSEEKE+ DPSPPAADFQLVRP SKFVKRSPIPSPPRG GSH
Sbjct: 181 WTGKSSKKAGAKALRSEGTSEEKEIHDPSPPAADFQLVRPPSKFVKRSPIPSPPRGNGSH 240

Query: 241 LRVEASQAIAESRELKFTSIENMKLTELKALAKSRGIKGYSKLKKNELMEVLGS 287
            RV+A+QAIAESRELKF SI+NMKLTELKALAKSRGIKGYSKLKKNEL+E+LGS
Sbjct: 241 PRVDATQAIAESRELKFPSIKNMKLTELKALAKSRGIKGYSKLKKNELIELLGS 279

[Close]

BLAST of Lsi02G008240.1 vs. NCBI nr
Match: XP_038893912.1 (SAP-like protein BP-73 isoform X3 [Benincasa hispida])

HSP 1 Score: 382.1 bits (980), Expect = 4.2e-102
Identity = 222/292 (76.03%), Postives = 238/292 (81.51%), Query Frame = 0


Query: 1   MEAIVFQPRILIRFPNLVSLGRRPTFASKGIKLETPKSQGTSLEFRKSIVCPFHSAFLLN 60
           MEA++FQPRILIRFP LVSLGRRPTFASK                               
Sbjct: 1   MEAVIFQPRILIRFPKLVSLGRRPTFASK------------------------------- 60

Query: 61  LADIADVYPSKSIQLSVTNNWPDGNAGNRPPRRISAPGKRRKNEPSSRKTEAH-KEEDLK 120
             DIADVYPSK IQLSV+NN PDG AGNRPPRRISAPGK RKNEPSSRKTEAH  EEDLK
Sbjct: 61  --DIADVYPSKGIQLSVSNNRPDGKAGNRPPRRISAPGKTRKNEPSSRKTEAHMNEEDLK 120

Query: 121 KPKFNNQEEIIALFRKIQTSIAKESATS-DDESCKDEQQGADSILETLRESRKQVK---- 180
           K K+NNQEEIIALFRKI+TSIAKESA+S D+ESCKDE  GA+SILETLRESRKQVK    
Sbjct: 121 KSKYNNQEEIIALFRKIETSIAKESASSNDEESCKDE-HGAESILETLRESRKQVKELWT 180

Query: 181 GKTSKKAGAKVLRSKGTSEEKEMQDPSPPAADFQLVRPLSKFVKRSPIPSPPRGRGSHLR 240
           GK+SKKAGAK LRS+GTSEEKE+ DPSPPAADFQLVRP SKFVKRSPIPSPPRG GSH R
Sbjct: 181 GKSSKKAGAKALRSEGTSEEKEIHDPSPPAADFQLVRPPSKFVKRSPIPSPPRGNGSHPR 240

Query: 241 VEASQAIAESRELKFTSIENMKLTELKALAKSRGIKGYSKLKKNELMEVLGS 287
           V+A+QAIAESRELKF SI+NMKLTELKALAKSRGIKGYSKLKKNEL+E+LGS
Sbjct: 241 VDATQAIAESRELKFPSIKNMKLTELKALAKSRGIKGYSKLKKNELIELLGS 258

[Close]

BLAST of Lsi02G008240.1 vs. NCBI nr
Match: KGN65614.1 (hypothetical protein Csa_019894 [Cucumis sativus])

HSP 1 Score: 321.2 bits (822), Expect = 8.8e-84
Identity = 200/289 (69.20%), Postives = 218/289 (75.43%), Query Frame = 0


Query: 1   MEAIVFQPRILIRFPNLVSLGRRPTFASKGIKLETPKSQGTSLEFRKSIVCPFHSAFLLN 60
           MEA+VF PRILIRFPNL+SL RRPTFASK                               
Sbjct: 1   MEAVVFLPRILIRFPNLISLRRRPTFASK------------------------------- 60

Query: 61  LADIADVYPSKSIQLSVTNNWPDGNAGNRPPRRISAPGKRRKNEPSSRKTEAHK-EEDLK 120
             D+ADVYPSK+IQ SV+ + PDGNAGNRPPRR S PGK RK+E SSRKTE  K EE +K
Sbjct: 61  --DVADVYPSKNIQPSVSKSRPDGNAGNRPPRRNSLPGKARKSESSSRKTETPKDEESVK 120

Query: 121 KPKFNNQEEIIALFRKIQTSIAKESATS-DDESCKDEQQGADSILETLRESRKQVKGKTS 180
           K + N+QEE+IALFRKIQTSIAKESA+S D+ES KDE     SILETLRESRKQ+KGKTS
Sbjct: 121 KSETNDQEEVIALFRKIQTSIAKESASSIDEESRKDENA---SILETLRESRKQLKGKTS 180

Query: 181 KKAGAKVLRSKGTSEEKEMQDPS-PPAADFQLVRPLSKFVKRSPIPSPPRGRGSHLRVEA 240
           KKAGAKVLRSKG SEEKEM DPS PPAADF+LVRP SKFVKRSPIP         L+V+A
Sbjct: 181 KKAGAKVLRSKGKSEEKEMHDPSPPPAADFKLVRPPSKFVKRSPIP---------LKVDA 240

Query: 241 SQAIAESRELKFTSIENMKLTELKALAKSRGIKGYSKLKKNELMEVLGS 287
           SQAIAESRELKF S ENMKLTELKALAKSRGIKGYSKLKKNELME+L S
Sbjct: 241 SQAIAESRELKFPSTENMKLTELKALAKSRGIKGYSKLKKNELMEILRS 244

[Close]

BLAST of Lsi02G008240.1 vs. NCBI nr
Match: XP_008446702.1 (PREDICTED: SAP-like protein BP-73 [Cucumis melo] >KAA0057053.1 SAP-like protein BP-73 [Cucumis melo var. makuwa])

HSP 1 Score: 303.5 bits (776), Expect = 1.9e-78
Identity = 184/244 (75.41%), Postives = 198/244 (81.15%), Query Frame = 0


Query: 45  FRKSIVCPFHSAFLLNLADIADVYPSKSIQLSVTNNWPDGNAGNRPPRRISAPGKRRKNE 104
           F K     F S  LLNLADIADVYPSK+IQLSV+NN PDGNA NRPPRR S PGK RK+E
Sbjct: 42  FEKKTHVRFES--LLNLADIADVYPSKNIQLSVSNNRPDGNARNRPPRRNSLPGKTRKSE 101

Query: 105 PSSRKTEAHK-EEDLKKPKFNNQEEIIALFRKIQTSIAKESATSDDESCKDEQQGADSIL 164
            SSRK EA K EE++KK K N+QEEIIALFRKIQ SIAKESA+S DE    ++ GA SIL
Sbjct: 102 SSSRKKEAPKNEENIKKSKPNDQEEIIALFRKIQASIAKESASSIDEESHKDEHGATSIL 161

Query: 165 ETLRESRKQVKGKTSKKAGAKVLRSKGTSEEKEMQDPS-PPAADFQLVRPLSKFVKRSPI 224
           ETLRE RKQ+KGKTSKKAGAKV RSKGTSEEKEM DPS PPAADF+LVRP SKFVKRSPI
Sbjct: 162 ETLREPRKQLKGKTSKKAGAKVFRSKGTSEEKEMHDPSPPPAADFKLVRPPSKFVKRSPI 221

Query: 225 PSPPRGRGSHLRVEASQAIAESRELKFTSIENMKLTELKALAKSRGIKGYSKLKKNELME 284
           P          +V+ASQAIAESRELKF SIENMKL ELKALAKSRGIKGYSKLKKNELME
Sbjct: 222 PP---------KVDASQAIAESRELKFPSIENMKLAELKALAKSRGIKGYSKLKKNELME 274

Query: 285 VLGS 287
           +L S
Sbjct: 282 ILRS 274

[Close]

BLAST of Lsi02G008240.1 vs. TAIR 10
Match: AT4G18740.1 (Rho termination factor )

HSP 1 Score: 92.0 bits (227), Expect = 8.1e-19
Identity = 76/192 (39.58%), Postives = 106/192 (55.21%), Query Frame = 0


Query: 104 EPSSRKTEAHKEEDLKKPKFNNQEEIIALFRKIQTSIAK------ESATSDDESCKDEQQ 163
           +PS R+T          P  +NQEEII+L ++IQ+SI+K      E   + DES K E+ 
Sbjct: 81  KPSDRRT--------SNPSKSNQEEIISLLKRIQSSISKGESRGVEEEKNSDESSK-EKP 140

Query: 164 GADSILETLRESRKQVKGKTSKKAGAKVLRSKGTSEEKEMQDPSPPAADFQLVRPLSKFV 223
              +IL+ L +SRK+ +G TS K                     PP    +L RP S FV
Sbjct: 141 LTKAILDVLEKSRKKTEGDTSVK-------------------EKPPKRQVELPRPPSSFV 200

Query: 224 KRSPIPSPPRGRGSHLRVEAS-QAIAE--SRELKFTSIENMKLTELKALAKSRGIKGYSK 283
           KR+P+ S   G    L V  S +A+ +   +E K + IE MKL ELK +AK+RGIKGYSK
Sbjct: 201 KRTPLSSSASGPRGKLPVSNSDKALGKLTKKEEKASLIETMKLAELKEVAKNRGIKGYSK 244

Query: 284 LKKNELMEVLGS 287
           L+K+EL+E++ S
Sbjct: 261 LRKSELLELIRS 244

[Close]

BLAST of Lsi02G008240.1 vs. TAIR 10
Match: AT4G18740.2 (Rho termination factor )

HSP 1 Score: 71.2 bits (173), Expect = 1.5e-12
Identity = 65/189 (34.39%), Postives = 90/189 (47.62%), Query Frame = 0


Query: 104 EPSSRKTEAHKEEDLKKPKFNNQEEIIALFRKIQTSIAK------ESATSDDESCKDEQQ 163
           +PS R+T          P  +NQEEII+L ++IQ+SI+K      E   + DES K E+ 
Sbjct: 81  KPSDRRT--------SNPSKSNQEEIISLLKRIQSSISKGESRGVEEEKNSDESSK-EKP 140

Query: 164 GADSILETLRESRKQVKGKTSKKAGAKVLRSKGTSEEKEMQDPSPPAADFQLVRPLSKFV 223
              +IL+ L +SRK+ +G TS K                     PP    +L RP S FV
Sbjct: 141 LTKAILDVLEKSRKKTEGDTSVK-------------------EKPPKRQVELPRPPSSFV 200

Query: 224 KRSPIPSPPRGRGSHLRVEASQAIAESRELKFTSIENMKLTELKALAKSRGIKGYSKLKK 283
           KR+P+ S   G                              ELK +AK+RGIKGYSKL+K
Sbjct: 201 KRTPLSSSASGP----------------------------RELKEVAKNRGIKGYSKLRK 213

Query: 284 NELMEVLGS 287
           +EL+E++ S
Sbjct: 261 SELLELIRS 213

[Close]

BLAST of Lsi02G008240.1 vs. TAIR 10
Match: AT4G18740.3 (Rho termination factor )

HSP 1 Score: 71.2 bits (173), Expect = 1.5e-12
Identity = 65/189 (34.39%), Postives = 90/189 (47.62%), Query Frame = 0


Query: 104 EPSSRKTEAHKEEDLKKPKFNNQEEIIALFRKIQTSIAK------ESATSDDESCKDEQQ 163
           +PS R+T          P  +NQEEII+L ++IQ+SI+K      E   + DES K E+ 
Sbjct: 56  KPSDRRT--------SNPSKSNQEEIISLLKRIQSSISKGESRGVEEEKNSDESSK-EKP 115

Query: 164 GADSILETLRESRKQVKGKTSKKAGAKVLRSKGTSEEKEMQDPSPPAADFQLVRPLSKFV 223
              +IL+ L +SRK+ +G TS K                     PP    +L RP S FV
Sbjct: 116 LTKAILDVLEKSRKKTEGDTSVK-------------------EKPPKRQVELPRPPSSFV 175

Query: 224 KRSPIPSPPRGRGSHLRVEASQAIAESRELKFTSIENMKLTELKALAKSRGIKGYSKLKK 283
           KR+P+ S   G                              ELK +AK+RGIKGYSKL+K
Sbjct: 176 KRTPLSSSASGP----------------------------RELKEVAKNRGIKGYSKLRK 188

Query: 284 NELMEVLGS 287
           +EL+E++ S
Sbjct: 236 SELLELIRS 188

[Close]

BLAST of Lsi02G008240.1 vs. TAIR 10
Match: AT1G06190.1 (Rho termination factor )

HSP 1 Score: 47.4 bits (111), Expect = 2.3e-05
Identity = 24/51 (47.06%), Postives = 35/51 (68.63%), Query Frame = 0


Query: 236 EASQAIAESRELKFTSIENMKLTELKALAKSRGIKGYSKLKKNELMEVLGS 287
           +A +   E+ E     +  +KL EL+ +AKSRG+KG SK+KK EL+E+LGS
Sbjct: 348 DADEDSDEAEEEAVKDLSELKLVELRGIAKSRGLKGLSKMKKAELVELLGS 398

The following BLAST results are available for this feature:

BLAST of Lsi02G008240.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94K75	3.2e-04	47.06	Rho-N domain-containing protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of Lsi02G008240.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LX66	4.3e-84	69.20	Rho_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G470300 PE=4 ...	[more]
A0A5A7UMB7	9.2e-79	75.41	SAP-like protein BP-73 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold9...	[more]
A0A1S3BFQ9	9.2e-79	75.41	SAP-like protein BP-73 OS=Cucumis melo OX=3656 GN=LOC103489342 PE=4 SV=1	[more]
A0A6J1CGN3	4.0e-74	63.89	uncharacterized protein LOC111010657 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1CF22	2.7e-70	71.19	uncharacterized protein LOC111010657 isoform X1 OS=Momordica charantia OX=3673 G...	[more]

BLAST of Lsi02G008240.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893911.1	1.2e-109	80.34	SAP-like protein BP-73 isoform X2 [Benincasa hispida]	[more]
XP_038893910.1	6.7e-108	79.25	SAP-like protein BP-73 isoform X1 [Benincasa hispida]	[more]
XP_038893912.1	4.2e-102	76.03	SAP-like protein BP-73 isoform X3 [Benincasa hispida]	[more]
KGN65614.1	8.8e-84	69.20	hypothetical protein Csa_019894 [Cucumis sativus]	[more]
XP_008446702.1	1.9e-78	75.41	PREDICTED: SAP-like protein BP-73 [Cucumis melo] >KAA0057053.1 SAP-like protein ...	[more]

BLAST of Lsi02G008240.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G18740.1	8.1e-19	39.58	Rho termination factor	[more]
AT4G18740.2	1.5e-12	34.39	Rho termination factor	[more]
AT4G18740.3	1.5e-12	34.39	Rho termination factor	[more]
AT1G06190.1	2.3e-05	47.06	Rho termination factor	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011112	Rho termination factor, N-terminal	SMART	SM00959	Rho_N_2_a	coord: 251..286 e-value: 8.0E-4 score: 28.7
IPR011112	Rho termination factor, N-terminal	PFAM	PF07498	Rho_N	coord: 253..282 e-value: 1.2E-8 score: 34.7
None	No IPR available	GENE3D	1.10.720.10		coord: 241..281 e-value: 3.2E-7 score: 32.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 97..123
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 78..123
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 186..200
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 167..206
None	No IPR available	PANTHER	PTHR34449:SF2	RHO TERMINATION FACTOR	coord: 1..285
None	No IPR available	PANTHER	PTHR34449	RHO TERMINATION FACTOR	coord: 1..285
IPR036269	Rho termination factor, N-terminal domain superfamily	SUPERFAMILY	68912	Rho N-terminal domain-like	coord: 248..282

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi02G008240	Lsi02G008240	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi02G008240.1.exon.3	Lsi02G008240.1.exon.3	exon
Lsi02G008240.1.exon.2	Lsi02G008240.1.exon.2	exon
Lsi02G008240.1.exon.1	Lsi02G008240.1.exon.1	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi02G008240.1.three_prime_UTR.1	Lsi02G008240.1.three_prime_UTR.1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi02G008240.1.CDS.3	Lsi02G008240.1.CDS.3	CDS
Lsi02G008240.1.CDS.2	Lsi02G008240.1.CDS.2	CDS
Lsi02G008240.1.CDS.1	Lsi02G008240.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi02G008240.1	Lsi02G008240.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006353	DNA-templated transcription, termination