Lsi02G007770.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi02G007770.1 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 1.4e-227
Identity = 395/616 (64.12%), Postives = 474/616 (76.95%), Query Frame = 0

Query: 4   NINRFDPQSAAEKAVSVIGFGYDLCNDIRLSYCKPGPSGSKLIDIDFTRTRDLVLPAGVV 63
           N  R D  SAAEKAVSVIG GYDLC+D+R S CK  P GS+L++ID TR RDL+ P G+V
Sbjct: 5   NFTRLDAHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIV 64

Query: 64  VPNVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCWQND 123
           V NVS+SI CDKGERTR RSD++SFNQMSE FNQ + LSGKIPSG FN MF    CW  D
Sbjct: 65  VNNVSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKD 124

Query: 124 AGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGV 183
           A S ++LA+DG FI+LY++E+ +  +TL ++VKREVPSSWD AALA FIEKYGTHVVVGV
Sbjct: 125 ASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGV 184

Query: 184 KMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPAPAKI-----KDE 243
            MGGKDVI ++Q+R+SN E  ++QK LK   DE+F  D       + +PA +     K+E
Sbjct: 185 TMGGKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDP----VESKSPASVYSGKPKEE 244

Query: 244 YSLPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISM 303
             L W L+  F  S+   V+ H+K +E+  + IRRGGVD+GQSH +WLST+SQ+PNVISM
Sbjct: 245 NLLQWGLQ-PFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISM 304

Query: 304 SFVPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRH 363
            FVPITSL++G+ G GFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAPVY DLPL  R 
Sbjct: 305 CFVPITSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRR 364

Query: 364 RKQASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKAL 423
            KQ+SPSL+F+LMGPKLYVNT+KVDS  RPVTG+R FLEGKK +HLAIHLQHLS  P +L
Sbjct: 365 SKQSSPSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSL 424

Query: 424 QLSDDVSYEPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIGDDFASIVTKAWFEVKV 483
            LS D +YEPI+EP EKGY+ PVKW IFSHVCT PV+YNG+   DD ASIVTKAW EVK 
Sbjct: 425 HLSHDDTYEPIEEPVEKGYYVPVKWGIFSHVCTYPVQYNGAR-SDDTASIVTKAWLEVKG 484

Query: 484 IGMRKVLFLRLGFSMVASAGIRRSDWEGPSMTSRKSGLISTFISTRFSTGLH----QPEE 543
           +GMRKVLFLRLGFS+ ASA  R+S W+  S  SRKSG+ S  ISTR STGL       + 
Sbjct: 485 MGMRKVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKP 544

Query: 544 QPKIELNSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISI 603
           Q KI++NSAVYP GP    +  K+L+++DTKE++RGPE  PGYWVVTGAKLCVE  +ISI
Sbjct: 545 QSKIDINSAVYPRGPSPPVKP-KLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISI 604

Query: 604 KVKYSLLTILSEDSMI 611
           K KYSLLT++SEDS++
Sbjct: 605 KAKYSLLTVISEDSLV 612

BLAST of Lsi02G007770.1 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 3.2e-179
Identity = 319/610 (52.30%), Postives = 421/610 (69.02%), Query Frame = 0

Query: 7   RFDPQSAAEKAVSVIGFGYDLCNDIRLSYCKPGPSGSKLIDI-DFTRTRDLVLPAGVVVP 66
           R     AAE A+  IG GYDL  D+RL YCK G   S+L+DI +     ++VLP G+ +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 126
           NVS SI CDKGER RFRSD++ F QM+E FNQ+LSL+GKIPSG FN MF    CWQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 STRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 186
            T++LAFDG FI+LY++ L+KS + L E VK+ VPS+WDPAALA FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDA---NGGFTSNPAPAKIKDEYSLP 246
           GGKDVI+ +Q   S ++  D+QK+LK++AD++F E +   N G     A +K++ +    
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQ-- 243

Query: 247 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVD-VGQSHNQWLSTISQSPNVISMSFV 306
             LR A  +S+     +++  ++   +  RRGG D     HN+WL T+   P+VISMSF+
Sbjct: 244 -RLRFADTSSLG----SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFI 303

Query: 307 PITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQ 366
           PITSL+NGV G+GFLSHA+NLYLRYKPPIEEL QFLEFQLPRQWAPV+++LPL P+ ++Q
Sbjct: 304 PITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQ 363

Query: 367 ASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKALQLS 426
           +  SL+F+  GPKLYVNTT VD   RP+TG+RL+LEG++S+ LAIHLQHLS+LPK  QL 
Sbjct: 364 SCASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLE 423

Query: 427 DDVSYEPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIGDDFASIVTKAWFEVKVIGM 486
           DD++     E  ++ Y+E V W  +SHVCT PVE       DD  S+VT A   V+  G 
Sbjct: 424 DDLNRSIRQESHDRRYYEKVNWKNYSHVCTEPVE------SDDDLSVVTGAQLHVESHGF 483

Query: 487 RKVLFLRLGFSMVASAG-IRRSDWEGPSMTSRKSGLISTFISTRFSTGLHQPEEQPKIEL 546
           + VLFLRL FS V  A  ++ S+W+     + KSGLIST IS  F+     P     + +
Sbjct: 484 KNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNI 543

Query: 547 NSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 606
           NSA+YP GPP+ ++A K+L  +DT EM RGP+ SPGYWVV+GA+L VE  +IS+KVKYSL
Sbjct: 544 NSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 600

Query: 607 LTILSEDSMI 611
            T +  D +I
Sbjct: 604 FTPILGDEVI 600

BLAST of Lsi02G007770.1 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 6.2e-130
Identity = 254/621 (40.90%), Postives = 377/621 (60.71%), Query Frame = 0

Query: 9   DPQSAAEKAVSVIGFGYDLCNDIRLSYCKPGPSGS---KLIDIDFTRTRDLVLPAGVVVP 68
           D     E AV  +G G+DL  D RL YCK G   +   +L+ +D T+ R+L +P   V  
Sbjct: 4   DGGDVIETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQ 63

Query: 69  NVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLK-GCWQNDA 128
           NVS  INCDKGERTRFRSD++ FN+MSE FNQ+ S++GKIPSG FN  FG + G W  DA
Sbjct: 64  NVSADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDA 123

Query: 129 GSTRSLAFDGCFITLYNIELEK-SHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGV 188
            + +SL  D   +TL+N+ +   + + L+++V+  VPSSWDP  LA FIE+YGTHV+ GV
Sbjct: 124 ANVKSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGV 183

Query: 189 KMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDA-------NGGFTSNPAPAKIK 248
            +GG+DV+ +RQ + S+++   ++  L  L D+ F+          N  +  + +  K  
Sbjct: 184 SVGGQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFP 243

Query: 249 DEYSLPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVI 308
           + +++     +    +     IN   G  +T I  +RGG    +SH++WL T+   P+ I
Sbjct: 244 EAFNV---FDDKQTVAFNNFSINSQNG--ITVICAKRGGDGRAKSHSEWLITVPDKPDAI 303

Query: 309 SMSFVPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPL-A 368
           + +F+PITSL+  V G+G LSHA++LYLRYKPP+ +LQ FL+F  PR WAPV+ DLP  A
Sbjct: 304 NFNFIPITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGA 363

Query: 369 PRHRKQASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLP 428
             +   A P+L    MGPKLYVNTT V S   PVTG+R FLEGKK + LAIHLQHL    
Sbjct: 364 APNMASAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTR 423

Query: 429 KAL--QLSDDVSYEPIDE-PEEKGYFEPVKWSIFSHVCTAPVEYNGSLI--------GDD 488
             +  +++D+  +   D+  +   YFEP+    FSHVCT PV+Y+ + I         +D
Sbjct: 424 TTVGEKITDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQND 483

Query: 489 FASIVTKAWFEVKVIGMRKVLFLRLGFSMVASAGIRRSDW-EGPSMTSRKSGLISTFIST 548
            A IVT A  EVK  G + VL LRL ++ V+   + ++ W  GP  TS+KSG+ S+    
Sbjct: 484 VAFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMP 543

Query: 549 RFSTGLHQ---PEEQPKIELNSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVV 602
             S  +H     +++ ++ L+S V+P GPP+ +   K++  +D  ++ RGP++SPG+W+V
Sbjct: 544 LTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLV 603

BLAST of Lsi02G007770.1 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 8.4e-119
Identity = 240/593 (40.47%), Postives = 348/593 (58.68%), Query Frame = 0

Query: 17  AVSVIGFGYDLCNDIRLSYCKPGPSGSKLIDIDFTRTRDLVLPAGVVVPNVSTSINCDKG 76
           A+  +G G+D+ +D+RL YCK  P GS+L+ I+  + RDL L  G ++PNV   I+C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKGAP-GSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 77  ERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCWQNDAGSTRSLAFDGCF 136
                R  V SF++M+E FN +  + G IP G FN MF   G WQ DA ST+SLA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 137 ITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKDVIFMRQL 196
           I LY+++L K  + L  +++R VPSSWDPA+LA FIE YGTH+V  V +GG+DV+++RQ 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 197 RESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPAPAKIKDEYSLPWELRNAFAASIKPP 256
           + S +  ++++  +  +   +F E  +   T    P K KD+                  
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHEAESQSIT---GPLKYKDK------------------ 263

Query: 257 VINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITSLMNGVRGNGFL 316
                   ++T I+ RRGG D+ QSH +W  T+  +P++I+M+F PI SL+ GV G   L
Sbjct: 264 --------DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 323

Query: 317 SHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPSLKFTLMGPKLY 376
           + A+ LYL YKPPIE+LQ FL++Q+ R WAP  ++L    + ++    SL+F+LMGPKL+
Sbjct: 324 TRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLF 383

Query: 377 VNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKALQLSDDVSYEPIDEPEEKG 436
           ++  +V    +PVTG+RL LEG K + L+IHLQHL +LPK LQ   D S+ PI  P+ +G
Sbjct: 384 ISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWD-SHVPIGAPKWQG 443

Query: 437 -------YFEPVKWSIFSHVCTAPVEYNGSLIGD-DFASIVTKAWFEVKVIGMRKVLFLR 496
                  +FEP+KW  FSHV T+P+E+  + IGD     IVT A   V   G + VL L+
Sbjct: 444 PEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLK 503

Query: 497 LGFSMVASAGIRRSDWEGPSMTSRKSGLISTFISTRFSTGLHQPEEQPKIELNSAVYPDG 556
           L FS V    IRRS W+   + S          S R   G   P      E         
Sbjct: 504 LLFSKVPGCTIRRSVWDHTPVAS----------SGRLEPG--GPSTSSSTE--------- 559

Query: 557 PPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLL 602
             ++ ++ K+  I+D+ EM++GP++ PG+W+VTGAKL VE  +I ++VKYSLL
Sbjct: 564 -EVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of Lsi02G007770.1 vs. ExPASy TrEMBL
Match: A0A5D3DSF4 (MACPF domain-containing protein NSL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G001020 PE=4 SV=1)

HSP 1 Score: 1194.1 bits (3088), Expect = 0.0e+00
Identity = 589/610 (96.56%), Postives = 597/610 (97.87%), Query Frame = 0

Query: 1   MSFNINRFDPQSAAEKAVSVIGFGYDLCNDIRLSYCKPGPSGSKLIDIDFTRTRDLVLPA 60
           MSFNINRF+PQSAAEKAVSV+GFGYDLCND+RLS CKPGPSGSKLIDIDFTR+RDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVVGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNV TSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPAPAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLAD+KFSEDANGGFTSN   AKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKALQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPK LQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDVSYEPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIGDDFASIVTKAWFEVKVIG 480
           SDD+SY PIDEPEEKGYFEPVKWSIFSHVCTAPVEY+ SLI DD ASIVTKAWFEVKVIG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSMTSRKSGLISTFISTRFSTGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPS TSRKSGLISTFISTRFS GLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLN+IDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Lsi02G007770.1 vs. ExPASy TrEMBL
Match: A0A0A0LV66 (MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G075560 PE=4 SV=1)

HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 589/610 (96.56%), Postives = 595/610 (97.54%), Query Frame = 0

Query: 1   MSFNINRFDPQSAAEKAVSVIGFGYDLCNDIRLSYCKPGPSGSKLIDIDFTRTRDLVLPA 60
           MSFNINRF+PQSAAEKAVSVIGFGYDLCND+RLS CKPGPSGSKLIDIDFTR+RDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNV TSINCDKGERTRFRSDVISFNQMSEL NQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPAPAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLAD+KFSEDANGG TSNP  AKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKALQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNN PVTGVRLFLEGKKSDHLAIHLQHLSTLPK LQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDVSYEPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIGDDFASIVTKAWFEVKVIG 480
           SDD+SY PIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLI DD ASIVTKAWFEVK IG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSMTSRKSGLISTFISTRFSTGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPS TSRKSGLISTFISTRFS GLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLN+IDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Lsi02G007770.1 vs. ExPASy TrEMBL
Match: A0A6J1JYX1 (MACPF domain-containing protein NSL1 OS=Cucurbita maxima OX=3661 GN=LOC111490102 PE=4 SV=1)

HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 560/610 (91.80%), Postives = 585/610 (95.90%), Query Frame = 0

Query: 1   MSFNINRFDPQSAAEKAVSVIGFGYDLCNDIRLSYCKPGPSGSKLIDIDFTRTRDLVLPA 60
           MSFN+NRFDPQSAAEKAVSVIGFG+DLCND+RLS CKPGPSGSKLIDID TRT+DLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVS SI CDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPS FFN+MFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGS++SL FDGCFITLYNIELE+SHITLSEQVKREVPSSWDPAALA+FIE YGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPAPAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESN+EQT+VQKKLK+LADE+FSEDANGGF SN A AKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQTEVQKKLKQLADERFSEDANGGFMSNSAAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +PW+LR+AFAASIKPP+I HSKGDEL  IY+RRGGVDVGQSHNQWLST+SQSPNVI MSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELMTIYVRRGGVDVGQSHNQWLSTVSQSPNVILMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA RHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKALQL 420
           QASPSLKFTLMGPKLYVNTTKVDSN+RPVTGVRLFLEGKKSDHLAIHLQHLSTLPK LQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDVSYEPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIGDDFASIVTKAWFEVKVIG 480
           SDD+SY PIDEPEEKGYF+PVKWSIFSHVCTAPV+YNGSLI DD ASIVTKAWFEVKVIG
Sbjct: 421 SDDLSYGPIDEPEEKGYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSMTSRKSGLISTFISTRFSTGLHQPEEQPKIEL 540
           MRKVLFLRLGFS VASA IRRS+WEGPS +SRKSGLISTFISTRFSTGL QPE QPKIEL
Sbjct: 481 MRKVLFLRLGFSTVASAMIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600
           NSAVYPDGPPLT RAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTVRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Lsi02G007770.1 vs. ExPASy TrEMBL
Match: A0A6J1GVB1 (MACPF domain-containing protein NSL1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457211 PE=4 SV=1)

HSP 1 Score: 1136.7 bits (2939), Expect = 0.0e+00
Identity = 554/610 (90.82%), Postives = 583/610 (95.57%), Query Frame = 0

Query: 1   MSFNINRFDPQSAAEKAVSVIGFGYDLCNDIRLSYCKPGPSGSKLIDIDFTRTRDLVLPA 60
           MSFN+NRFDPQSAAEKAVSVIGFG+DLCND+RLS CKPGPSGSKLIDID TRT+DLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVS SI CDKGERTRFRSDV+SFNQMSELFNQQLSLSGKIPS FFN+MFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVVSFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGS++SL FDGCFITLYNIELE+SHITLSEQVKREVPSSWDPAALA+FIE YGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPAPAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESN+EQ +VQKKLK+LADE+FSED NGGF SN   AKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDVNGGFMSNSTAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +PW+LR+AFAASIKPP+I HSKGDELT IY+RRGGVDVGQSHNQWLST+SQSPNVISMSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSL+NGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA R RK
Sbjct: 301 VPITSLLNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRLRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKALQL 420
           QASPSLKFTLMGPKLYVNTTKVDSN+RPVTGVRLFLEGKKSDHLAIHLQHLSTLPK +QL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQL 420

Query: 421 SDDVSYEPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIGDDFASIVTKAWFEVKVIG 480
           SDD+SY PIDEPEEKGYF+PVKWSIFSHVCTAPV+YNGSLI DD ASIVTKAWFEVKVIG
Sbjct: 421 SDDLSYGPIDEPEEKGYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSMTSRKSGLISTFISTRFSTGLHQPEEQPKIEL 540
           MRKVLFLRLGFS VASA IRRS+WEGPS +SRKSGLISTFISTRFSTGL QPE QPKIEL
Sbjct: 481 MRKVLFLRLGFSTVASAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600
           NSAVYPDGPPLT RAMKMLN+IDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTVRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Lsi02G007770.1 vs. ExPASy TrEMBL
Match: A0A5A7UMD4 (MACPF domain-containing protein NSL1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G001940 PE=4 SV=1)

HSP 1 Score: 1123.6 bits (2905), Expect = 0.0e+00
Identity = 564/610 (92.46%), Postives = 578/610 (94.75%), Query Frame = 0

Query: 1   MSFNINRFDPQSAAEKAVSVIGFGYDLCNDIRLSYCKPGPSGSKLIDIDFTRTRDLVLPA 60
           MSFNINRF+PQSAAEKAVSV+GFGYDLCND+RLS CKPGPSGSKLIDIDFTR+RDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVVGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNV TSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPAPAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLAD+KFSEDANGGFTSN   AKIK    
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIK---- 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +P +  + + +   P   +H    ELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 VPTQ-EHKWLSPFGPFHWSH----ELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKALQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPK LQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDVSYEPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIGDDFASIVTKAWFEVKVIG 480
           SDD+SY PIDEPEEKGYFEPVKWSIFSHVCTAPVEY+ SLI DD ASIVTKAWFEVKVIG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSMTSRKSGLISTFISTRFSTGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPS TSRKSGLISTFISTRFS GLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLN+IDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 601

BLAST of Lsi02G007770.1 vs. NCBI nr
Match: XP_038894492.1 (MACPF domain-containing protein NSL1 [Benincasa hispida])

HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 597/610 (97.87%), Postives = 602/610 (98.69%), Query Frame = 0

Query: 1   MSFNINRFDPQSAAEKAVSVIGFGYDLCNDIRLSYCKPGPSGSKLIDIDFTRTRDLVLPA 60
           MSFNINRFDPQSAAEKAVSVIGFGYDLCND+RLS CKPGPSGSKLIDIDFTRTRDLVLP 
Sbjct: 1   MSFNINRFDPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRTRDLVLPG 60

Query: 61  GVVVPNVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPAPAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPA AKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPAAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKALQL 420
           QASPSLKFTLMGPKLYVNTTKVDS+NRPVTGVRLFLEGKKSDHLAIHLQHLSTLPK LQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSHNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDVSYEPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIGDDFASIVTKAWFEVKVIG 480
           SDD+SY PIDEPEEKGYFEPV+WSIFSHVCTAPVEYNGSLI DD ASIVTKAWFEVKVIG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVRWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSMTSRKSGLISTFISTRFSTGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPS TSRKSGLISTFISTRFS+GLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSSGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Lsi02G007770.1 vs. NCBI nr
Match: TYK26438.1 (MACPF domain-containing protein NSL1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1194.1 bits (3088), Expect = 0.0e+00
Identity = 589/610 (96.56%), Postives = 597/610 (97.87%), Query Frame = 0

Query: 1   MSFNINRFDPQSAAEKAVSVIGFGYDLCNDIRLSYCKPGPSGSKLIDIDFTRTRDLVLPA 60
           MSFNINRF+PQSAAEKAVSV+GFGYDLCND+RLS CKPGPSGSKLIDIDFTR+RDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVVGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNV TSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPAPAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLAD+KFSEDANGGFTSN   AKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGFTSNSVAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKALQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPK LQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDVSYEPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIGDDFASIVTKAWFEVKVIG 480
           SDD+SY PIDEPEEKGYFEPVKWSIFSHVCTAPVEY+ SLI DD ASIVTKAWFEVKVIG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYHDSLIDDDSASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSMTSRKSGLISTFISTRFSTGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPS TSRKSGLISTFISTRFS GLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLN+IDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Lsi02G007770.1 vs. NCBI nr
Match: XP_004146657.1 (MACPF domain-containing protein NSL1 [Cucumis sativus] >KGN64702.1 hypothetical protein Csa_013635 [Cucumis sativus])

HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 589/610 (96.56%), Postives = 595/610 (97.54%), Query Frame = 0

Query: 1   MSFNINRFDPQSAAEKAVSVIGFGYDLCNDIRLSYCKPGPSGSKLIDIDFTRTRDLVLPA 60
           MSFNINRF+PQSAAEKAVSVIGFGYDLCND+RLS CKPGPSGSKLIDIDFTR+RDLVLPA
Sbjct: 1   MSFNINRFNPQSAAEKAVSVIGFGYDLCNDLRLSSCKPGPSGSKLIDIDFTRSRDLVLPA 60

Query: 61  GVVVPNVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNV TSINCDKGERTRFRSDVISFNQMSEL NQQLSLSGKIPSGFFNTMFGLKGCW
Sbjct: 61  GVVVPNVPTSINCDKGERTRFRSDVISFNQMSELCNQQLSLSGKIPSGFFNTMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV
Sbjct: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPAPAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLAD+KFSEDANGG TSNP  AKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADDKFSEDANGGLTSNPVAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF
Sbjct: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKALQL 420
           QASPSLKFTLMGPKLYVNTTKVDSNN PVTGVRLFLEGKKSDHLAIHLQHLSTLPK LQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNNLPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDVSYEPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIGDDFASIVTKAWFEVKVIG 480
           SDD+SY PIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLI DD ASIVTKAWFEVK IG
Sbjct: 421 SDDLSYRPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIDDDSASIVTKAWFEVKGIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSMTSRKSGLISTFISTRFSTGLHQPEEQPKIEL 540
           MRKVLFLRLGFSMVASAGIRRSDWEGPS TSRKSGLISTFISTRFS GLHQPEEQPKIEL
Sbjct: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSTTSRKSGLISTFISTRFSAGLHQPEEQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600
           NSAVYPDGPPLTSRAMKMLN+IDTKEMVRGPENSPGYWVVTGAKLCVEG RISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTSRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGGRISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Lsi02G007770.1 vs. NCBI nr
Match: XP_022994356.1 (MACPF domain-containing protein NSL1 [Cucurbita maxima])

HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 560/610 (91.80%), Postives = 585/610 (95.90%), Query Frame = 0

Query: 1   MSFNINRFDPQSAAEKAVSVIGFGYDLCNDIRLSYCKPGPSGSKLIDIDFTRTRDLVLPA 60
           MSFN+NRFDPQSAAEKAVSVIGFG+DLCND+RLS CKPGPSGSKLIDID TRT+DLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVS SI CDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPS FFN+MFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGS++SL FDGCFITLYNIELE+SHITLSEQVKREVPSSWDPAALA+FIE YGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALAKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPAPAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESN+EQT+VQKKLK+LADE+FSEDANGGF SN A AKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQTEVQKKLKQLADERFSEDANGGFMSNSAAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +PW+LR+AFAASIKPP+I HSKGDEL  IY+RRGGVDVGQSHNQWLST+SQSPNVI MSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELMTIYVRRGGVDVGQSHNQWLSTVSQSPNVILMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA RHRK
Sbjct: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKALQL 420
           QASPSLKFTLMGPKLYVNTTKVDSN+RPVTGVRLFLEGKKSDHLAIHLQHLSTLPK LQL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTLQL 420

Query: 421 SDDVSYEPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIGDDFASIVTKAWFEVKVIG 480
           SDD+SY PIDEPEEKGYF+PVKWSIFSHVCTAPV+YNGSLI DD ASIVTKAWFEVKVIG
Sbjct: 421 SDDLSYGPIDEPEEKGYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSMTSRKSGLISTFISTRFSTGLHQPEEQPKIEL 540
           MRKVLFLRLGFS VASA IRRS+WEGPS +SRKSGLISTFISTRFSTGL QPE QPKIEL
Sbjct: 481 MRKVLFLRLGFSTVASAMIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600
           NSAVYPDGPPLT RAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTVRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Lsi02G007770.1 vs. NCBI nr
Match: KAG6573287.1 (MACPF domain-containing protein NSL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 556/610 (91.15%), Postives = 585/610 (95.90%), Query Frame = 0

Query: 1   MSFNINRFDPQSAAEKAVSVIGFGYDLCNDIRLSYCKPGPSGSKLIDIDFTRTRDLVLPA 60
           MSFN+NRFDPQSAAEKAVSVIGFG+DLCND+RLS CKPGPSGSKLIDID TRT+DLVLPA
Sbjct: 1   MSFNVNRFDPQSAAEKAVSVIGFGFDLCNDLRLSSCKPGPSGSKLIDIDLTRTKDLVLPA 60

Query: 61  GVVVPNVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCW 120
           GVVVPNVS SI CDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPS FFN+MFGLKGCW
Sbjct: 61  GVVVPNVSNSIKCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSAFFNSMFGLKGCW 120

Query: 121 QNDAGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVV 180
           QNDAGS++SL FDGCFITLYNIELE+SHITLSEQVKREVPSSWDPAAL++FIE YGTHVV
Sbjct: 121 QNDAGSSKSLGFDGCFITLYNIELERSHITLSEQVKREVPSSWDPAALSKFIENYGTHVV 180

Query: 181 VGVKMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPAPAKIKDEYS 240
           VGVKMGGKDVIFMRQLRESN+EQ +VQKKLK+LADE+FSEDANGGF SN   AKIKDEYS
Sbjct: 181 VGVKMGGKDVIFMRQLRESNIEQAEVQKKLKQLADERFSEDANGGFMSNSTAAKIKDEYS 240

Query: 241 LPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSF 300
           +PW+LR+AFAASIKPP+I HSKGDELT IY+RRGGVDVGQSHNQWLST+SQSPNVISMSF
Sbjct: 241 MPWQLRHAFAASIKPPIITHSKGDELTTIYVRRGGVDVGQSHNQWLSTVSQSPNVISMSF 300

Query: 301 VPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRK 360
           VPITSL+NGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLA RHRK
Sbjct: 301 VPITSLLNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLATRHRK 360

Query: 361 QASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKALQL 420
           QASPSLKFTLMGPKLYVNTTKVDSN+RPVTGVRLFLEGKKSDHLAIHLQHLSTLPK +QL
Sbjct: 361 QASPSLKFTLMGPKLYVNTTKVDSNSRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKTIQL 420

Query: 421 SDDVSYEPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIGDDFASIVTKAWFEVKVIG 480
           SDD+SY PIDEPEEKGYF+PVKWSIFSHVCTAPV+YNGSLI DD ASIVTKAWFEVKVIG
Sbjct: 421 SDDLSYGPIDEPEEKGYFQPVKWSIFSHVCTAPVQYNGSLIDDDTASIVTKAWFEVKVIG 480

Query: 481 MRKVLFLRLGFSMVASAGIRRSDWEGPSMTSRKSGLISTFISTRFSTGLHQPEEQPKIEL 540
           MRKVLFLRLGFS VASA IRRS+WEGPS +SRKSGLISTFISTRFSTGL QPE QPKIEL
Sbjct: 481 MRKVLFLRLGFSTVASAKIRRSEWEGPSTSSRKSGLISTFISTRFSTGLQQPEAQPKIEL 540

Query: 541 NSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600
           NSAVYPDGPPLT RAMKMLN+IDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL
Sbjct: 541 NSAVYPDGPPLTVRAMKMLNVIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 600

Query: 601 LTILSEDSMI 611
           LTILSEDSMI
Sbjct: 601 LTILSEDSMI 610

BLAST of Lsi02G007770.1 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 790.4 bits (2040), Expect = 1.0e-228
Identity = 395/616 (64.12%), Postives = 474/616 (76.95%), Query Frame = 0

Query: 4   NINRFDPQSAAEKAVSVIGFGYDLCNDIRLSYCKPGPSGSKLIDIDFTRTRDLVLPAGVV 63
           N  R D  SAAEKAVSVIG GYDLC+D+R S CK  P GS+L++ID TR RDL+ P G+V
Sbjct: 5   NFTRLDAHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIV 64

Query: 64  VPNVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCWQND 123
           V NVS+SI CDKGERTR RSD++SFNQMSE FNQ + LSGKIPSG FN MF    CW  D
Sbjct: 65  VNNVSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKD 124

Query: 124 AGSTRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGV 183
           A S ++LA+DG FI+LY++E+ +  +TL ++VKREVPSSWD AALA FIEKYGTHVVVGV
Sbjct: 125 ASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGV 184

Query: 184 KMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPAPAKI-----KDE 243
            MGGKDVI ++Q+R+SN E  ++QK LK   DE+F  D       + +PA +     K+E
Sbjct: 185 TMGGKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDP----VESKSPASVYSGKPKEE 244

Query: 244 YSLPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISM 303
             L W L+  F  S+   V+ H+K +E+  + IRRGGVD+GQSH +WLST+SQ+PNVISM
Sbjct: 245 NLLQWGLQ-PFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISM 304

Query: 304 SFVPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRH 363
            FVPITSL++G+ G GFLSHAVNLYLRYKPPIEEL QFLEFQLPRQWAPVY DLPL  R 
Sbjct: 305 CFVPITSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRR 364

Query: 364 RKQASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKAL 423
            KQ+SPSL+F+LMGPKLYVNT+KVDS  RPVTG+R FLEGKK +HLAIHLQHLS  P +L
Sbjct: 365 SKQSSPSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSL 424

Query: 424 QLSDDVSYEPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIGDDFASIVTKAWFEVKV 483
            LS D +YEPI+EP EKGY+ PVKW IFSHVCT PV+YNG+   DD ASIVTKAW EVK 
Sbjct: 425 HLSHDDTYEPIEEPVEKGYYVPVKWGIFSHVCTYPVQYNGAR-SDDTASIVTKAWLEVKG 484

Query: 484 IGMRKVLFLRLGFSMVASAGIRRSDWEGPSMTSRKSGLISTFISTRFSTGLH----QPEE 543
           +GMRKVLFLRLGFS+ ASA  R+S W+  S  SRKSG+ S  ISTR STGL       + 
Sbjct: 485 MGMRKVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKP 544

Query: 544 QPKIELNSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISI 603
           Q KI++NSAVYP GP    +  K+L+++DTKE++RGPE  PGYWVVTGAKLCVE  +ISI
Sbjct: 545 QSKIDINSAVYPRGPSPPVKP-KLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISI 604

Query: 604 KVKYSLLTILSEDSMI 611
           K KYSLLT++SEDS++
Sbjct: 605 KAKYSLLTVISEDSLV 612

BLAST of Lsi02G007770.1 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 629.8 bits (1623), Expect = 2.3e-180
Identity = 319/610 (52.30%), Postives = 421/610 (69.02%), Query Frame = 0

Query: 7   RFDPQSAAEKAVSVIGFGYDLCNDIRLSYCKPGPSGSKLIDI-DFTRTRDLVLPAGVVVP 66
           R     AAE A+  IG GYDL  D+RL YCK G   S+L+DI +     ++VLP G+ +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 126
           NVS SI CDKGER RFRSD++ F QM+E FNQ+LSL+GKIPSG FN MF    CWQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 STRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 186
            T++LAFDG FI+LY++ L+KS + L E VK+ VPS+WDPAALA FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDA---NGGFTSNPAPAKIKDEYSLP 246
           GGKDVI+ +Q   S ++  D+QK+LK++AD++F E +   N G     A +K++ +    
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQ-- 243

Query: 247 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVD-VGQSHNQWLSTISQSPNVISMSFV 306
             LR A  +S+     +++  ++   +  RRGG D     HN+WL T+   P+VISMSF+
Sbjct: 244 -RLRFADTSSLG----SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFI 303

Query: 307 PITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQ 366
           PITSL+NGV G+GFLSHA+NLYLRYKPPIEEL QFLEFQLPRQWAPV+++LPL P+ ++Q
Sbjct: 304 PITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQ 363

Query: 367 ASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKALQLS 426
           +  SL+F+  GPKLYVNTT VD   RP+TG+RL+LEG++S+ LAIHLQHLS+LPK  QL 
Sbjct: 364 SCASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLE 423

Query: 427 DDVSYEPIDEPEEKGYFEPVKWSIFSHVCTAPVEYNGSLIGDDFASIVTKAWFEVKVIGM 486
           DD++     E  ++ Y+E V W  +SHVCT PVE       DD  S+VT A   V+  G 
Sbjct: 424 DDLNRSIRQESHDRRYYEKVNWKNYSHVCTEPVE------SDDDLSVVTGAQLHVESHGF 483

Query: 487 RKVLFLRLGFSMVASAG-IRRSDWEGPSMTSRKSGLISTFISTRFSTGLHQPEEQPKIEL 546
           + VLFLRL FS V  A  ++ S+W+     + KSGLIST IS  F+     P     + +
Sbjct: 484 KNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNI 543

Query: 547 NSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSL 606
           NSA+YP GPP+ ++A K+L  +DT EM RGP+ SPGYWVV+GA+L VE  +IS+KVKYSL
Sbjct: 544 NSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 600

Query: 607 LTILSEDSMI 611
            T +  D +I
Sbjct: 604 FTPILGDEVI 600

BLAST of Lsi02G007770.1 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 466.1 bits (1198), Expect = 4.4e-131
Identity = 254/621 (40.90%), Postives = 377/621 (60.71%), Query Frame = 0

Query: 9   DPQSAAEKAVSVIGFGYDLCNDIRLSYCKPGPSGS---KLIDIDFTRTRDLVLPAGVVVP 68
           D     E AV  +G G+DL  D RL YCK G   +   +L+ +D T+ R+L +P   V  
Sbjct: 4   DGGDVIETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQ 63

Query: 69  NVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLK-GCWQNDA 128
           NVS  INCDKGERTRFRSD++ FN+MSE FNQ+ S++GKIPSG FN  FG + G W  DA
Sbjct: 64  NVSADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDA 123

Query: 129 GSTRSLAFDGCFITLYNIELEK-SHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGV 188
            + +SL  D   +TL+N+ +   + + L+++V+  VPSSWDP  LA FIE+YGTHV+ GV
Sbjct: 124 ANVKSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGV 183

Query: 189 KMGGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDA-------NGGFTSNPAPAKIK 248
            +GG+DV+ +RQ + S+++   ++  L  L D+ F+          N  +  + +  K  
Sbjct: 184 SVGGQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFP 243

Query: 249 DEYSLPWELRNAFAASIKPPVINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVI 308
           + +++     +    +     IN   G  +T I  +RGG    +SH++WL T+   P+ I
Sbjct: 244 EAFNV---FDDKQTVAFNNFSINSQNG--ITVICAKRGGDGRAKSHSEWLITVPDKPDAI 303

Query: 309 SMSFVPITSLMNGVRGNGFLSHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPL-A 368
           + +F+PITSL+  V G+G LSHA++LYLRYKPP+ +LQ FL+F  PR WAPV+ DLP  A
Sbjct: 304 NFNFIPITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGA 363

Query: 369 PRHRKQASPSLKFTLMGPKLYVNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLP 428
             +   A P+L    MGPKLYVNTT V S   PVTG+R FLEGKK + LAIHLQHL    
Sbjct: 364 APNMASAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTR 423

Query: 429 KAL--QLSDDVSYEPIDE-PEEKGYFEPVKWSIFSHVCTAPVEYNGSLI--------GDD 488
             +  +++D+  +   D+  +   YFEP+    FSHVCT PV+Y+ + I         +D
Sbjct: 424 TTVGEKITDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQND 483

Query: 489 FASIVTKAWFEVKVIGMRKVLFLRLGFSMVASAGIRRSDW-EGPSMTSRKSGLISTFIST 548
            A IVT A  EVK  G + VL LRL ++ V+   + ++ W  GP  TS+KSG+ S+    
Sbjct: 484 VAFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMP 543

Query: 549 RFSTGLHQ---PEEQPKIELNSAVYPDGPPLTSRAMKMLNIIDTKEMVRGPENSPGYWVV 602
             S  +H     +++ ++ L+S V+P GPP+ +   K++  +D  ++ RGP++SPG+W+V
Sbjct: 544 LTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLV 603

BLAST of Lsi02G007770.1 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 429.1 bits (1102), Expect = 6.0e-120
Identity = 240/593 (40.47%), Postives = 348/593 (58.68%), Query Frame = 0

Query: 17  AVSVIGFGYDLCNDIRLSYCKPGPSGSKLIDIDFTRTRDLVLPAGVVVPNVSTSINCDKG 76
           A+  +G G+D+ +D+RL YCK  P GS+L+ I+  + RDL L  G ++PNV   I+C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKGAP-GSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 77  ERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCWQNDAGSTRSLAFDGCF 136
                R  V SF++M+E FN +  + G IP G FN MF   G WQ DA ST+SLA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 137 ITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKMGGKDVIFMRQL 196
           I LY+++L K  + L  +++R VPSSWDPA+LA FIE YGTH+V  V +GG+DV+++RQ 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 197 RESNVEQTDVQKKLKKLADEKFSEDANGGFTSNPAPAKIKDEYSLPWELRNAFAASIKPP 256
           + S +  ++++  +  +   +F E  +   T    P K KD+                  
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHEAESQSIT---GPLKYKDK------------------ 263

Query: 257 VINHSKGDELTAIYIRRGGVDVGQSHNQWLSTISQSPNVISMSFVPITSLMNGVRGNGFL 316
                   ++T I+ RRGG D+ QSH +W  T+  +P++I+M+F PI SL+ GV G   L
Sbjct: 264 --------DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 323

Query: 317 SHAVNLYLRYKPPIEELQQFLEFQLPRQWAPVYADLPLAPRHRKQASPSLKFTLMGPKLY 376
           + A+ LYL YKPPIE+LQ FL++Q+ R WAP  ++L    + ++    SL+F+LMGPKL+
Sbjct: 324 TRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLF 383

Query: 377 VNTTKVDSNNRPVTGVRLFLEGKKSDHLAIHLQHLSTLPKALQLSDDVSYEPIDEPEEKG 436
           ++  +V    +PVTG+RL LEG K + L+IHLQHL +LPK LQ   D S+ PI  P+ +G
Sbjct: 384 ISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWD-SHVPIGAPKWQG 443

Query: 437 -------YFEPVKWSIFSHVCTAPVEYNGSLIGD-DFASIVTKAWFEVKVIGMRKVLFLR 496
                  +FEP+KW  FSHV T+P+E+  + IGD     IVT A   V   G + VL L+
Sbjct: 444 PEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLK 503

Query: 497 LGFSMVASAGIRRSDWEGPSMTSRKSGLISTFISTRFSTGLHQPEEQPKIELNSAVYPDG 556
           L FS V    IRRS W+   + S          S R   G   P      E         
Sbjct: 504 LLFSKVPGCTIRRSVWDHTPVAS----------SGRLEPG--GPSTSSSTE--------- 559

Query: 557 PPLTSRAMKMLNIIDTKEMVRGPENSPGYWVVTGAKLCVEGARISIKVKYSLL 602
             ++ ++ K+  I+D+ EM++GP++ PG+W+VTGAKL VE  +I ++VKYSLL
Sbjct: 564 -EVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of Lsi02G007770.1 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 331.6 bits (849), Expect = 1.3e-90
Identity = 169/327 (51.68%), Postives = 225/327 (68.81%), Query Frame = 0

Query: 7   RFDPQSAAEKAVSVIGFGYDLCNDIRLSYCKPGPSGSKLIDI-DFTRTRDLVLPAGVVVP 66
           R     AAE A+  IG GYDL  D+RL YCK G   S+L+DI +     ++VLP G+ +P
Sbjct: 4   RLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIP 63

Query: 67  NVSTSINCDKGERTRFRSDVISFNQMSELFNQQLSLSGKIPSGFFNTMFGLKGCWQNDAG 126
           NVS SI CDKGER RFRSD++ F QM+E FNQ+LSL+GKIPSG FN MF    CWQ DA 
Sbjct: 64  NVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAA 123

Query: 127 STRSLAFDGCFITLYNIELEKSHITLSEQVKREVPSSWDPAALAEFIEKYGTHVVVGVKM 186
            T++LAFDG FI+LY++ L+KS + L E VK+ VPS+WDPAALA FI+ YGTH++V VKM
Sbjct: 124 YTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKM 183

Query: 187 GGKDVIFMRQLRESNVEQTDVQKKLKKLADEKFSEDA---NGGFTSNPAPAKIKDEYSLP 246
           GGKDVI+ +Q   S ++  D+QK+LK++AD++F E +   N G     A +K++ +    
Sbjct: 184 GGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQ-- 243

Query: 247 WELRNAFAASIKPPVINHSKGDELTAIYIRRGGVD-VGQSHNQWLSTISQSPNVISMSFV 306
             LR A  +S+     +++  ++   +  RRGG D     HN+WL T+   P+VISMSF+
Sbjct: 244 -RLRFADTSSLG----SYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFI 303

Query: 307 PITSLMNGVRGNGFLSHAVNLYLRYKP 329
           PITSL+NGV G+GFLSHA+NLYLR KP
Sbjct: 304 PITSLLNGVPGSGFLSHAINLYLRCKP 323