Lsi02G006690.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G006690.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	(3S,6E)-nerolidol synthase 1-like
Location	chr02: 5987233 .. 5992813 (-)
Sequence length	543
RNA-Seq Expression	Lsi02G006690.1
Synteny	Lsi02G006690.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGGTTAAAGAGAAGACGTAAAAGTGATTTACTTATAGTATTCATTGGATTTGTGTCTATCCACGCTATGATGTTAGGATCACACATTTTCTTGCTTGAATGTATTCCCACATAATTCAATTGACGGGTTATACATCGTTTGCCAACTAATTTATACCTTTTTGGACAACCATTTAATTTTTTTTTCAAAAAAATTAATTTTATAAATACTAATTAATTCAATTGCTCAGATTGATTGAGCACGTGAAAAAGATAGTAGGAGTAGATTATAGTCTTCTAAGTAAAGAAACATGATTGTCGATGAAGAGAAATAAAATTTTCAAATATCTTGTCAGAGTGGAATTTTGGTAATCTTTTGACAAAATATCGGACGAGTAGTTGTTAGAAATGAGAAAGTTATGGACTCCAAACACTATAAAAAGAGCTTATGACTTAAGTTTTAAATCGTCCAAGTTATAAATATTTTAAGTTTGGGTGTACTTTTTTTCATTTTTTTATTCTCTAGTTTGTAAGTAGGAAAAAAATGTGAGTGATATACAAAAGTTTTGAGGTAATGTTCTTGTAATTGAGATTTGTGAAGAAAATTCTATGTGATTATAATAATTTTTTACATAGTGGATATTTGACTGTAGTTTTTCTCCAGTTTAGGAGTTTTCAAGTAAATTCTTGTGTTTTCTATTCTTGTTGTTTCTTTTAATTTCTCAATTAATTTCTACTTGGTACACGTTGTAGATGTTTTTTTTTTTCAAAATTAAGAATGTTTTCAAAATCGAATCAAATTTTAAAGACTAAAAAAATGCAGTTTTTGAAGAAAAAAAACTTTTGGAATTTGAATTAAAATTCAATTAATTATTAGGAAAAATAAATTTGTGCCATGGAGTTTGGGAAAAAAGAAGCACAGTTTTAAAAAATAGAAAATAAAAACTAAATTATCTAACCTACTTTTTAGTTTTTGATTTTTAAAAATTGTGTTATTTTCTCACAATTCTCCACAATGTTTTCCATCTTTCCTCAAGAAACATTTGTATTTATAGCCAAACTTTTTTTTTTAAAACATATTATTTAATTTTTTATTTTTTAAAACTTTGCTAAGTAGAAAACATTCCTAAAAAGTATATAGAAACAAAATTATTGGTGAAGTATATATCAACTTTGTTTTCAAAATTAAAAAACAAAAGCCTAAGATCCTCTCGGATAACAATTTGGTTTTTGTTGTAAAATCGAGAAGAAAAACGGACACCACAACAATACAAATATTAGAGAAATATAATATAATAATAAAATATATATAAAATAATTTAAATAAAAAACTCTCCAAAATTTTCCACTATTTCCAAACTTTTTGGATTTCTCACCAATGTGTGTATCTCATAAAATCCACAAAATGTCACTATTTATAGGCAATTTGTCAAGTAGGAGGTGGATTACATGGACACTAACAGTGGGTTATCTTGAGACACATGGATAATATATGGGTAAATATAGGATTGACACATGGCCAATGGATACTAAGCATGAACACCTAATTATATTTTTGTTTTAATGTCTACTTTTTAGAAGTGTTTTTAAAATCTAAGTTAAAGTATTAAAAGCAAAGATAATTAAAAGAAAAAAAACACAAATGGGCTAAGAATTCAATCTTATTAAAAATGAAAATCATGCTAGGAAAAATTGTGAGAAAAGAATGTACAATTTTCTCAACATATTTCTAAAATGAAGTCATGGGCTATTTCTAGCCATATTTATTTATTCTGGCAAAATTTTCTCAACAAAAGAAAAGCATGAAGTACAATTTTCTACTTATTGCTATTTACAAGGTTTATTTCAACCTTCTATTTCTAGCTATATATTTGTTAGGTTTTCACAAAATATTAGAAATTTAGTTGTTTCACATCTAAAAAGTTTAAGGAAGTTAGAGCTCCAAATACCATAAAAGAAATTTATGCTTTTAGTTTCAAATCGTCGCAGTAAGCTTAGGTGTGCTAAATATAGTTAAATTAATTCTCTTTTGTATTCTCCAATTTGAAAATGGAAAAAATGGTGTGAATAGTATAAAATCTTTGTGAGAGTACTCTTGTAATTGAGATTATAAGAGAAAATTATTCTGTATGATTGTAATAATTTTTCACATAGTGATTTCTTTTTTGGTGGGTCATAGTTTTTCTTCGAGTTTAGCTTATTCTAGTGGGAAATTTTTTTTCAACAATATTCACATGGAAGACGATCAACAAGAACCAGAAAGCAATAATCTATCTCAGAAATTCAACATGTCCACACGAAAAAATACTCTCAACACGTATGAGAACAACACATTAAATACCCAAGAGGCCAACTAGTCTACTACATGATTATGTAATGGACTAATATATTGAAATTATCGCACATCTGCTCCACAAGGGACTTGACAATATTCAAATTCACCTTACTACATTCAATTTGTCCATTCCAAATTGTCATAATTCTTATCTGTCAGACTATTTAATAAATTTATGTTGACAGTTTGGAATGGACAAATTTATTTTTATGGACATTTCACCACCAAGGAACATGAAACACGTGTGCGGTAGAAATAAGGAGACACTGACACATCATTTTTTAAAAAAATTAAAACCAGACATAATAAGCCATATTTATTAAAATATTATTTTATACACGAAATATTAAATCAATATTTTTAAGAGAATTTTTAAAAATAGAAAAATTTTGAAAATTATTTACACAAAATAACAAAATTTTTAAGTAGTTGTAATAGACGGATAGAAATGATAGAAGTCTATCCCTGATAGATGCTAATAGAAGTCTATCAATATCTATTCATATTTTTTTTATTATTTTTTTATAAATAATTTGATATTTTTTCTACGTGTAAAAATTTCTCATATGTGTATGCATTTCTGTTGGACCCTCGTAGATTTTAAACCTGCGATACTCCCACTCGTGTTAAACACAAATATGTTAACAAACTAAATGTTTATGCTTCTTAGTTCATTAATTAATTATATTTATTATGTTTAAGCAAGTGTACTATAAATGCCAACTAGTGTTTGCGTATATCTATTGTGCACAACTTAACCAATGTTTAACGCATGTATTTATTCCCCTCCCAAAAAAATGCATGTATTTATTTATTTGTTTATTTATTAATTTTTTGAAACAATCCAAATAAAAGAATTACATGGTTCTATGACCAAATAAGAAAAGAGAAATCTTTTGAATACAATTATACAAAGAGACTACCCATGACGGGCCAAAATCACGTCATGGCGCACTCAATCCGGAACTACCTTCTTTCGCCAGGGCATGTGTCATGGTATTGGAACTGCGCGACACGTAATGAAAGCGCACTCAAAGAAGACTCAATCACCCCACCAAAGCCCACCACCAAAGAGCCTGTCTCACCAGCCGCATCCACATGTAATATGCTCAAATGTTTATATGAGATATTAAACATTGGAGGAACTCCTTATATAGATGAAGAATAAACGCATGTATTTATTGTGGCAAAAAGAGTAATATTTTAAATAAGATTATCTTCACGCATATATTTATTGTGGTAAAACGTGTGATATGTTTAAATAACATTATTAAGATTATTTAAGAAGTTTCTTGAAACGCAATTGGAACTATTGGATCTTCCATAAAGTACAAGGCCAATTTGTGGCTATTAAATTCTTCACATTGTTCAAGGAAGATTTGGAGTCATTGGACTTGCTTGATAATCAAATTTATTGGCTATAAATAGGTGGAGACAATATAAAGAGTTTCTTCTTATCAGAGAGTTTATTATTCTTTTATAAGAAAGAAAGTCTGTCACTTCTATTTTATATAGTGAAATTCTTCTATCCTTGCATGTAGTTTTTATCCTAATTTATTTAGGAGTTTTTAACATAAAATCACCTTGTGTTATTCTCTCATTTCTTCTTCTCCATTATTACTATTCTCAATCTATTTTAGCTGCAATCTTAATCTATTCGATCACAATCCTTTCACAACATGATACGTGTCTAAAACGTGACCATGCTTCTTAACCCTAACGATGAATTAATGTTACAATTATGCAAAAATATGGCAAACACATGTGTTATATGGCCCATGCTACATACTGCAATAATAAGTTTCGTAGTCTATAAGAAAGCATAGAGAGGGGTAAGGTCGACACACTATCATCATATCATCAGTCTCTTCTTCATCAAAACACAAAAGGAATATTTGAAACTCTAGTGGCAAATCAAAATCCTCCTCTCATTATTAGTATACTCATATATTCATATATTCATATTCTATGGCCCAACTCATTTATCATCAACTCTTCCTTGCTTCTCCCTGCCTAAAAATATTCATATCTCAAACAAAAATTCCTCACAAAGTTTACACTACCAACGCATGGCTGCGATTGAATAATCCTCTTGAAAGTTCTTTTGCTAAAAAATGGAGCATTCATGCCTTGGATTCTTCTTCCTCGAAGCTGATCTCCAATTTGAAATTTGTATATATTATTATATATTATTTTAGCTGATTGATTTTTTCTCTTTTTATTTTTTATTTTTTTTATATATGATGCCATGATGGGTGTGTGTAGGAGGAGTTTGGGCTTGAAACTCCATTGGGTAAAGTGAAGATTTTAAAGGAAATGCTAAGCCAAACAAGAGATTTGTGTTGGGAATGTTTGGAAATAATTGATGCAGCCCAACGGCTTGGCATTGACAATCACTTCCAAGAGGAGATTGAGGCTGTTCTTAAGAGTCAATACGATTTTATGAATGCAAACCATTTTGATGGAGACATGGATCTTCACAAAGCTGCCCTTCTTTTTCGACTTTTGAGACAAGGATACCTTGTGTCCGCAGGTTCAGATTCCACTTTTAAACTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTCTTTTTTTTTTCTTGAGTGATTTTAGAATGATGGCTTGTTTGGACCCTTTTAAATCACTTCCAGTATGTTAAATATTGAGAAACTATTATAAATAGAAAAAATATCAAACTATATTTGTAAATCGTTTGATAATTTCTATTTCTAAAAATAGCCCTAAAATATTAATACAAGTTTGGGAGTGATTTTGAAATTGTTAAAAATCATTTATGTCATGTTTCAAAATCACTCTCAACACGTCTTTAATCACTTAAAGTTAATTTCATATTTTATTTTACACTTTTAAATACAATTTTCATGTCTTACAAATTGGTTTTGAATGACTAAAAGTATGTTTAAGAATGATTTTGAAAATAAAAAAGTGATTTTTTAAAATTATTTTGACTATTATAGTGCATGTTTGGAGTTAATTTTGAAATTGTTATAATCACTTTTATCATGTTTGAAATTACTCTCAAACACTCCTTTAATCACTCAAAATTAATTTAATTAACAATTAGTTTTACACTTTTAAATATAATTTTCCTATCACATTTCCATGTTAAAATAGATGTTTGATTATCATAATGTAATCTTGGCCAATTACTTGAGAGAGGCTTTTTCTTTTTTCTTTTTTTTTTTTTTTTAAAGTGATTGATAGAGATTTTATTTTTAAAATCTATTAA

mRNA sequence

ATGATGTATACTCATATATTCATATATTCATATTCTATGGCCCAACTCATTTATCATCAACTCTTCCTTGCTTCTCCCTGCCTAAAAATATTCATATCTCAAACAAAAATTCCTCACAAAGTTTACACTACCAACGCATGGCTGCGATTGAATAATCCTCTTGAAAGTTCTTTTGCTAAAAAATGGAGCATTCATGCCTTGGATTCTTCTTCCTCGAAGCTGATCTCCAATTTGAAATTTGAGGAGTTTGGGCTTGAAACTCCATTGGGTAAAGTGAAGATTTTAAAGGAAATGCTAAGCCAAACAAGAGATTTGTGTTGGGAATGTTTGGAAATAATTGATGCAGCCCAACGGCTTGGCATTGACAATCACTTCCAAGAGGAGATTGAGGCTGTTCTTAAGAGTCAATACGATTTTATGAATGCAAACCATTTTGATGGAGACATGGATCTTCACAAAGCTGCCCTTCTTTTTCGACTTTTGAGACAAGGATACCTTGTGTCCGCAGTGATTGATAGAGATTTTATTTTTAAAATCTATTAA

Coding sequence (CDS)

ATGATGTATACTCATATATTCATATATTCATATTCTATGGCCCAACTCATTTATCATCAACTCTTCCTTGCTTCTCCCTGCCTAAAAATATTCATATCTCAAACAAAAATTCCTCACAAAGTTTACACTACCAACGCATGGCTGCGATTGAATAATCCTCTTGAAAGTTCTTTTGCTAAAAAATGGAGCATTCATGCCTTGGATTCTTCTTCCTCGAAGCTGATCTCCAATTTGAAATTTGAGGAGTTTGGGCTTGAAACTCCATTGGGTAAAGTGAAGATTTTAAAGGAAATGCTAAGCCAAACAAGAGATTTGTGTTGGGAATGTTTGGAAATAATTGATGCAGCCCAACGGCTTGGCATTGACAATCACTTCCAAGAGGAGATTGAGGCTGTTCTTAAGAGTCAATACGATTTTATGAATGCAAACCATTTTGATGGAGACATGGATCTTCACAAAGCTGCCCTTCTTTTTCGACTTTTGAGACAAGGATACCTTGTGTCCGCAGTGATTGATAGAGATTTTATTTTTAAAATCTATTAA

Protein sequence

MMYTHIFIYSYSMAQLIYHQLFLASPCLKIFISQTKIPHKVYTTNAWLRLNNPLESSFAKKWSIHALDSSSSKLISNLKFEEFGLETPLGKVKILKEMLSQTRDLCWECLEIIDAAQRLGIDNHFQEEIEAVLKSQYDFMNANHFDGDMDLHKAALLFRLLRQGYLVSAVIDRDFIFKIY

Homology

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BLAST of Lsi02G006690.1 vs. ExPASy Swiss-Prot
Match: B9RXW4 (Probable terpene synthase 13 OS=Ricinus communis OX=3988 GN=TPS13 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.1e-09
Identity = 56/151 (37.09%), Postives = 80/151 (52.98%), Query Frame = 0


Query: 23  LASPCLKIFISQTKIPHKVYTTNAWLRLNN-PLESSF-AKKWSIH-ALDSSSSKLISNLK 82
           +A   + IFI     P     T+  L  N+   +S+F    WSI+  L S  S    +L 
Sbjct: 1   MALSSISIFIHCLVDPLPRKQTSKTLPSNHIDFKSAFNVANWSINQTLVSPLSAQCIHLT 60

Query: 83  FEEFGLETPLGKVKILKEMLSQTRDLCWECLEIIDAAQRLGIDNHFQEEIEAVLKSQYDF 142
            E+  ++    + +  K +L +  +   E L +IDA QRLGID+HFQ+EI+ +L+ QY  
Sbjct: 61  DEDVRIKE--RRTRAFKHILRKEGEGSHEVLAMIDAIQRLGIDHHFQDEIDEILQRQYTI 120

Query: 143 MNANHFDGDMDLHKAALLFRLLRQ-GYLVSA 170
            +   +  D DLH  AL FRLLRQ GY VSA
Sbjct: 121 PS---YYNDNDLHGLALRFRLLRQGGYNVSA 146

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BLAST of Lsi02G006690.1 vs. ExPASy Swiss-Prot
Match: Q84UV0 (S-(+)-linalool synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TPS14 PE=2 SV=2)

HSP 1 Score: 62.0 bits (149), Expect = 7.9e-09
Identity = 47/117 (40.17%), Postives = 65/117 (55.56%), Query Frame = 0


Query: 63  SIHALDSSSSKLISNLKFEEFGLETPLGKVKILKEMLSQTRDLCWECLEIIDAAQRLGID 122
           ++HA  S+ SK   N    E+  E     +K +K +LS   D+  E LE+ID  Q LGID
Sbjct: 50  NVHASVSNPSKQFHNKTSLEYLHEL---NIKKIKNILSANVDVPSENLEMIDVIQSLGID 109

Query: 123 NHFQEEIEAVLKSQYDFMNANHFDGDMDLHKAALLFRLLRQ-GYLVSAVIDRDFIFK 179
            HF++EIE  L   Y       F+G  DLH+ AL FRLLRQ G+ V  +I ++ + K
Sbjct: 110 LHFRQEIEQTLHMIY--KEGLQFNG--DLHEIALRFRLLRQEGHYVQEIIFKNILDK 159

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BLAST of Lsi02G006690.1 vs. ExPASy Swiss-Prot
Match: P0CV94 ((3S,6E)-nerolidol synthase 1 OS=Fragaria ananassa OX=3747 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 3.9e-08
Identity = 38/87 (43.68%), Postives = 52/87 (59.77%), Query Frame = 0


Query: 91  KVKILKEMLSQTRDL-CWECLEIIDAAQRLGIDNHFQEEIEAVLKSQYDFMNANHFDGDM 150
           K+++LK +L    +L   E L +IDA QRLGID +FQ EI+ +L  Q   ++A       
Sbjct: 21  KLELLKTVLRNVAELDALEGLNMIDAVQRLGIDYNFQREIDEILHKQMSIVSARD----- 80

Query: 151 DLHKAALLFRLLRQ-GYLVSAVIDRDF 176
           DLH+ AL FRLLRQ GY V   +  +F
Sbjct: 81  DLHEVALRFRLLRQHGYFVPEDVFNNF 102

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BLAST of Lsi02G006690.1 vs. ExPASy Swiss-Prot
Match: P0CV95 ((3S,6E)-nerolidol synthase 2, chloroplastic/mitochondrial OS=Fragaria ananassa OX=3747 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 6.7e-08
Identity = 37/87 (42.53%), Postives = 52/87 (59.77%), Query Frame = 0


Query: 91  KVKILKEMLSQTRDL-CWECLEIIDAAQRLGIDNHFQEEIEAVLKSQYDFMNANHFDGDM 150
           K+++ + +L    +L   E L +IDA QRLGID HFQ EI+ +L  Q   ++A+      
Sbjct: 80  KLELFRNVLRNVAELDALEGLNMIDAVQRLGIDFHFQREIDEILHKQMSNVSASD----- 139

Query: 151 DLHKAALLFRLLRQ-GYLVSAVIDRDF 176
           DLH+ AL FRLLRQ GY V   +  +F
Sbjct: 140 DLHEVALRFRLLRQHGYFVPEDVFNNF 161

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BLAST of Lsi02G006690.1 vs. ExPASy Swiss-Prot
Match: J7LJN5 (Beta-caryophyllene synthase OS=Phyla dulcis OX=542674 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 6.7e-08
Identity = 37/81 (45.68%), Postives = 51/81 (62.96%), Query Frame = 0


Query: 95  LKEMLSQTRDLCWECLEIIDAAQRLGIDNHFQEEIEAVLKSQY-----DFMNANHFDGDM 154
           ++++L  T D   + LE+IDA QRLG+D+HFQ+EIE  L+  Y     D  + +H     
Sbjct: 55  VEKLLGATHDDSLQKLELIDAIQRLGVDHHFQKEIEKSLQDIYLRCCNDKDDYDH--NQT 114

Query: 155 DLHKAALLFRLLR-QGYLVSA 170
           DLH  AL FRLLR QGY +S+
Sbjct: 115 DLHTVALRFRLLRQQGYNISS 133

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BLAST of Lsi02G006690.1 vs. ExPASy TrEMBL
Match: A0A1S4E662 ((3S,6E)-nerolidol synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103489227 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 2.3e-51
Identity = 120/170 (70.59%), Postives = 136/170 (80.00%), Query Frame = 0


Query: 13  MAQLIYHQLFLA-SPCLKIFISQTKIPHKVYTTNAWLRLNNPLESSFAKKWSIHALDSSS 72
           MAQLIY Q FLA  P  KIFISQTKIPHK +  NAWL+LNNPLE SFA+K  IHAL+SSS
Sbjct: 1   MAQLIYLQSFLAFPPRPKIFISQTKIPHKAHNNNAWLQLNNPLEKSFAEKGFIHALNSSS 60

Query: 73  SK---LISNLKFEEFGLETPLGKVKILKEMLSQTR-DLCWECLEIIDAAQRLGIDNHFQE 132
           S    +IS+LK EE G+ETP  KVKILKE+L++TR DLC ECLEIIDAAQRLGIDN F+E
Sbjct: 61  SSNKIIISDLKIEELGVETPARKVKILKELLTETRDDLCLECLEIIDAAQRLGIDNQFEE 120

Query: 133 EIEAVLKSQYDFMNANHFDGDMDLHKAALLFRLLR-QGYLVSAVIDRDFI 177
           EIE VLK QY  +NA HFDGD+DLHKAALLFRLLR Q YLVS  + + F+
Sbjct: 121 EIEDVLKRQYALINAYHFDGDVDLHKAALLFRLLRQQRYLVSQDVFKVFL 170

[Close]

BLAST of Lsi02G006690.1 vs. ExPASy TrEMBL
Match: A0A0A0LX82 (Terpene_synth domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G070080 PE=4 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 3.3e-50
Identity = 113/157 (71.97%), Postives = 128/157 (81.53%), Query Frame = 0


Query: 17  IYHQLFLASPCLKIFISQTKIPHKVYTTNAWLRLNNPLESSFAKKWSIHALDSSSSK--- 76
           +Y Q FLASPC KIFI QTK PHKV   NAWL+LN+PLE SFA+K  IHAL+SSSS    
Sbjct: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC 60

Query: 77  LISNLKFEEFGLETPLGKVKILKEMLSQTR-DLCWECLEIIDAAQRLGIDNHFQEEIEAV 136
           +ISNLK EEFG+ETP  KV+ILK++L++TR DLCWE LEIIDAAQ LGIDN F+EEIE V
Sbjct: 61  IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV 120

Query: 137 LKSQYDFMNANHFDGDMDLHKAALLFRLLR-QGYLVS 169
           LK QYD +NA  FDGDMDLHKAALLFRLLR Q YL+S
Sbjct: 121 LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLIS 157

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BLAST of Lsi02G006690.1 vs. ExPASy TrEMBL
Match: A0A5D3BI68 ((3S,6E)-nerolidol synthase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G006300 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 5.7e-18
Identity = 58/98 (59.18%), Postives = 70/98 (71.43%), Query Frame = 0


Query: 84  GLETPLGKVK--ILKEMLSQTRDLCWECLEIIDAAQRLGIDNHFQEEIEAVLKSQYDFMN 143
           G+E   G +K  ILK++L +  D  WECL +IDA QRLGID HFQEEIEAVL+ QY   N
Sbjct: 19  GIEYGKGAIKGRILKDVLREIGD-PWECLNLIDATQRLGIDYHFQEEIEAVLQRQYVLFN 78

Query: 144 ANHFDGDMDLHKAALLFRLLR-QGYLVSAVIDRDFIFK 179
           A  F+ + DLHKAALLFRL R QGYLVSA + + F+ K
Sbjct: 79  AVQFNPETDLHKAALLFRLFRQQGYLVSADVFKSFMDK 115

[Close]

BLAST of Lsi02G006690.1 vs. ExPASy TrEMBL
Match: A0A1S3B3I5 ((3S,6E)-nerolidol synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103485759 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 5.7e-18
Identity = 66/135 (48.89%), Postives = 84/135 (62.22%), Query Frame = 0


Query: 50  LNNPLESSFAKKWSIHALDSSSSKLISNL---KFEEFGLETPLGKVK--ILKEMLSQTRD 109
           +NNPL +        H+L S+  K + +         G+E   G +K  ILK++L +  D
Sbjct: 32  INNPLLNKQIISLPHHSLLSTPLKPLHSKIQPSILTNGIEYGKGAIKGRILKDVLREIGD 91

Query: 110 LCWECLEIIDAAQRLGIDNHFQEEIEAVLKSQYDFMNANHFDGDMDLHKAALLFRLLR-Q 169
             WECL +IDA QRLGID HFQEEIEAVL+ QY   NA  F+ + DLHKAALLFRL R Q
Sbjct: 92  -PWECLNLIDATQRLGIDYHFQEEIEAVLQRQYVLFNAVQFNPETDLHKAALLFRLFRQQ 151

Query: 170 GYLVSAVIDRDFIFK 179
           GYLVSA + + F+ K
Sbjct: 152 GYLVSADVFKSFMDK 165

[Close]

BLAST of Lsi02G006690.1 vs. ExPASy TrEMBL
Match: A0A0A0LUC8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G068570 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 9.7e-18
Identity = 55/89 (61.80%), Postives = 66/89 (74.16%), Query Frame = 0


Query: 91  KVKILKEMLSQTRDLCWECLEIIDAAQRLGIDNHFQEEIEAVLKSQYDFMNANHFDGDMD 150
           K +ILK++L +  D  WECL +IDA QRLGID HFQEEIEAVL+ QY   NA  ++ D D
Sbjct: 74  KGRILKDVLREIGD-PWECLNLIDATQRLGIDYHFQEEIEAVLQRQYVLFNAIQYNPDTD 133

Query: 151 LHKAALLFRLLR-QGYLVSAVIDRDFIFK 179
           LHKAALLFRL R QGYLVSA + + F+ K
Sbjct: 134 LHKAALLFRLFRQQGYLVSADVFKSFMDK 161

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BLAST of Lsi02G006690.1 vs. NCBI nr
Match: XP_038893983.1 ((3S,6E)-nerolidol synthase 1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 239.6 bits (610), Expect = 2.1e-59
Identity = 128/166 (77.11%), Postives = 138/166 (83.13%), Query Frame = 0


Query: 13  MAQLIYHQLFLASPCLKIFISQTKIPHKVYTTNAWLRL-NNPLESSFAKKWSIHALDSSS 72
           MAQLIYHQ  LAS   KI +SQTKIPHKV+   A L+L NNP    FAKKWSI+ALDSSS
Sbjct: 1   MAQLIYHQPILASSTPKILMSQTKIPHKVHHKKALLQLKNNPPRKPFAKKWSIYALDSSS 60

Query: 73  SKLISNLKFEEFGLETPLGKVKILKEMLSQTRDLCWECLEIIDAAQRLGIDNHFQEEIEA 132
           SK ISNLK EEFGLE P GKVKILKE L QTRDLCWECLEIIDAAQRLGIDNHF+EEIE 
Sbjct: 61  SKPISNLKIEEFGLEAPDGKVKILKEALKQTRDLCWECLEIIDAAQRLGIDNHFKEEIET 120

Query: 133 VLKSQYDFMNANHFDGDMDLHKAALLFRLLR-QGYLVSAVIDRDFI 177
           +LK QYD +NANHFDGDMDLHKAALLFRLLR QGYLVSA + + F+
Sbjct: 121 ILKRQYDLINANHFDGDMDLHKAALLFRLLRQQGYLVSADMFKVFL 166

[Close]

BLAST of Lsi02G006690.1 vs. NCBI nr
Match: XP_038893982.1 ((3S,6E)-nerolidol synthase 1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 239.6 bits (610), Expect = 2.1e-59
Identity = 128/166 (77.11%), Postives = 138/166 (83.13%), Query Frame = 0


Query: 13  MAQLIYHQLFLASPCLKIFISQTKIPHKVYTTNAWLRL-NNPLESSFAKKWSIHALDSSS 72
           MAQLIYHQ  LAS   KI +SQTKIPHKV+   A L+L NNP    FAKKWSI+ALDSSS
Sbjct: 1   MAQLIYHQPILASSTPKILMSQTKIPHKVHHKKALLQLKNNPPRKPFAKKWSIYALDSSS 60

Query: 73  SKLISNLKFEEFGLETPLGKVKILKEMLSQTRDLCWECLEIIDAAQRLGIDNHFQEEIEA 132
           SK ISNLK EEFGLE P GKVKILKE L QTRDLCWECLEIIDAAQRLGIDNHF+EEIE 
Sbjct: 61  SKPISNLKIEEFGLEAPDGKVKILKEALKQTRDLCWECLEIIDAAQRLGIDNHFKEEIET 120

Query: 133 VLKSQYDFMNANHFDGDMDLHKAALLFRLLR-QGYLVSAVIDRDFI 177
           +LK QYD +NANHFDGDMDLHKAALLFRLLR QGYLVSA + + F+
Sbjct: 121 ILKRQYDLINANHFDGDMDLHKAALLFRLLRQQGYLVSADMFKVFL 166

[Close]

BLAST of Lsi02G006690.1 vs. NCBI nr
Match: XP_016903707.1 (PREDICTED: (3S,6E)-nerolidol synthase 1-like [Cucumis melo])

HSP 1 Score: 211.8 bits (538), Expect = 4.7e-51
Identity = 120/170 (70.59%), Postives = 136/170 (80.00%), Query Frame = 0


Query: 13  MAQLIYHQLFLA-SPCLKIFISQTKIPHKVYTTNAWLRLNNPLESSFAKKWSIHALDSSS 72
           MAQLIY Q FLA  P  KIFISQTKIPHK +  NAWL+LNNPLE SFA+K  IHAL+SSS
Sbjct: 1   MAQLIYLQSFLAFPPRPKIFISQTKIPHKAHNNNAWLQLNNPLEKSFAEKGFIHALNSSS 60

Query: 73  SK---LISNLKFEEFGLETPLGKVKILKEMLSQTR-DLCWECLEIIDAAQRLGIDNHFQE 132
           S    +IS+LK EE G+ETP  KVKILKE+L++TR DLC ECLEIIDAAQRLGIDN F+E
Sbjct: 61  SSNKIIISDLKIEELGVETPARKVKILKELLTETRDDLCLECLEIIDAAQRLGIDNQFEE 120

Query: 133 EIEAVLKSQYDFMNANHFDGDMDLHKAALLFRLLR-QGYLVSAVIDRDFI 177
           EIE VLK QY  +NA HFDGD+DLHKAALLFRLLR Q YLVS  + + F+
Sbjct: 121 EIEDVLKRQYALINAYHFDGDVDLHKAALLFRLLRQQRYLVSQDVFKVFL 170

[Close]

BLAST of Lsi02G006690.1 vs. NCBI nr
Match: XP_011652894.1 (myrcene synthase, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 208.0 bits (528), Expect = 6.8e-50
Identity = 113/157 (71.97%), Postives = 128/157 (81.53%), Query Frame = 0


Query: 17  IYHQLFLASPCLKIFISQTKIPHKVYTTNAWLRLNNPLESSFAKKWSIHALDSSSSK--- 76
           +Y Q FLASPC KIFI QTK PHKV   NAWL+LN+PLE SFA+K  IHAL+SSSS    
Sbjct: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC 60

Query: 77  LISNLKFEEFGLETPLGKVKILKEMLSQTR-DLCWECLEIIDAAQRLGIDNHFQEEIEAV 136
           +ISNLK EEFG+ETP  KV+ILK++L++TR DLCWE LEIIDAAQ LGIDN F+EEIE V
Sbjct: 61  IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV 120

Query: 137 LKSQYDFMNANHFDGDMDLHKAALLFRLLR-QGYLVS 169
           LK QYD +NA  FDGDMDLHKAALLFRLLR Q YL+S
Sbjct: 121 LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLIS 157

[Close]

BLAST of Lsi02G006690.1 vs. NCBI nr
Match: XP_011652895.1 (probable terpene synthase 13 isoform X2 [Cucumis sativus])

HSP 1 Score: 208.0 bits (528), Expect = 6.8e-50
Identity = 113/157 (71.97%), Postives = 128/157 (81.53%), Query Frame = 0


Query: 17  IYHQLFLASPCLKIFISQTKIPHKVYTTNAWLRLNNPLESSFAKKWSIHALDSSSSK--- 76
           +Y Q FLASPC KIFI QTK PHKV   NAWL+LN+PLE SFA+K  IHAL+SSSS    
Sbjct: 1   MYLQSFLASPCPKIFIYQTKNPHKVQNNNAWLQLNDPLEKSFARKGCIHALNSSSSSNKC 60

Query: 77  LISNLKFEEFGLETPLGKVKILKEMLSQTR-DLCWECLEIIDAAQRLGIDNHFQEEIEAV 136
           +ISNLK EEFG+ETP  KV+ILK++L++TR DLCWE LEIIDAAQ LGIDN F+EEIE V
Sbjct: 61  IISNLKIEEFGVETPARKVEILKDILTETRDDLCWERLEIIDAAQWLGIDNQFEEEIEDV 120

Query: 137 LKSQYDFMNANHFDGDMDLHKAALLFRLLR-QGYLVS 169
           LK QYD +NA  FDGDMDLHKAALLFRLLR Q YL+S
Sbjct: 121 LKRQYDLINAYRFDGDMDLHKAALLFRLLRQQRYLIS 157

[Close]

BLAST of Lsi02G006690.1 vs. TAIR 10
Match: AT1G61680.1 (terpene synthase 14 )

HSP 1 Score: 62.0 bits (149), Expect = 5.6e-10
Identity = 47/117 (40.17%), Postives = 65/117 (55.56%), Query Frame = 0


Query: 63  SIHALDSSSSKLISNLKFEEFGLETPLGKVKILKEMLSQTRDLCWECLEIIDAAQRLGID 122
           ++HA  S+ SK   N    E+  E     +K +K +LS   D+  E LE+ID  Q LGID
Sbjct: 50  NVHASVSNPSKQFHNKTSLEYLHEL---NIKKIKNILSANVDVPSENLEMIDVIQSLGID 109

Query: 123 NHFQEEIEAVLKSQYDFMNANHFDGDMDLHKAALLFRLLRQ-GYLVSAVIDRDFIFK 179
            HF++EIE  L   Y       F+G  DLH+ AL FRLLRQ G+ V  +I ++ + K
Sbjct: 110 LHFRQEIEQTLHMIY--KEGLQFNG--DLHEIALRFRLLRQEGHYVQEIIFKNILDK 159

[Close]

BLAST of Lsi02G006690.1 vs. TAIR 10
Match: AT1G61680.2 (terpene synthase 14 )

HSP 1 Score: 58.5 bits (140), Expect = 6.2e-09
Identity = 43/101 (42.57%), Postives = 56/101 (55.45%), Query Frame = 0


Query: 63  SIHALDSSSSKLISNLKFEEFGLETPLGKVKILKEMLSQTRDLCWECLEIIDAAQRLGID 122
           ++HA  S+ SK   N    E+  E     +K +K +LS   D+  E LE+ID  Q LGID
Sbjct: 50  NVHASVSNPSKQFHNKTSLEYLHEL---NIKKIKNILSANVDVPSENLEMIDVIQSLGID 109

Query: 123 NHFQEEIEAVLKSQYDFMNANHFDGDMDLHKAALLFRLLRQ 164
            HF++EIE  L   Y       F+G  DLH+ AL FRLLRQ
Sbjct: 110 LHFRQEIEQTLHMIY--KEGLQFNG--DLHEIALRFRLLRQ 143

[Close]

BLAST of Lsi02G006690.1 vs. TAIR 10
Match: AT2G24210.1 (terpene synthase 10 )

HSP 1 Score: 51.6 bits (122), Expect = 7.6e-07
Identity = 39/109 (35.78%), Postives = 56/109 (51.38%), Query Frame = 0


Query: 61  KWSIHALDSSSSKLISNLKFEEFGLETPLGKVKILKEMLSQTRDLCWECLEIIDAAQRLG 120
           +W  H L S  +K   + +      E  L K K+ ++ML+  +    + LE ID  Q+LG
Sbjct: 55  RWDHHHLLSVENKFAKDKRVR----ERDLLKEKV-RKMLNDEQKTYLDQLEFIDDLQKLG 114

Query: 121 IDNHFQEEIEAVLKSQYDFMNANHFDGDMDLHKAALLFRLLRQ-GYLVS 169
           +  HF+ EI+ +L S Y     N    + DLH  AL FRL RQ G+ VS
Sbjct: 115 VSYHFEAEIDNILTSSYKKDRTN--IQESDLHATALEFRLFRQHGFNVS 156

[Close]

BLAST of Lsi02G006690.1 vs. TAIR 10
Match: AT3G25810.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 48.9 bits (115), Expect = 4.9e-06
Identity = 37/91 (40.66%), Postives = 54/91 (59.34%), Query Frame = 0


Query: 93  KILKEMLSQTRDLCWECLEIIDAAQRLGIDNHFQEEIEAVLKSQYDFMNANHF---DGDM 152
           K+L+E+ +++R    E LE+ID  Q+LG+  HF++EI  +L + +   N  +    D + 
Sbjct: 87  KMLEEVETKSR---LEKLELIDDLQKLGVSYHFEQEINNIL-TNFHLENGKNIWKCDKEE 146

Query: 153 DLHKAALLFRLLRQ-GYLVSAVIDRDFIFKI 180
           DLH  AL FRLLRQ G+ VS  I    I KI
Sbjct: 147 DLHATALEFRLLRQHGFGVSEDIFDVIIDKI 173

[Close]

BLAST of Lsi02G006690.1 vs. TAIR 10
Match: AT3G25820.1 (terpene synthase-like sequence-1,8-cineole )

HSP 1 Score: 47.8 bits (112), Expect = 1.1e-05
Identity = 33/79 (41.77%), Postives = 44/79 (55.70%), Query Frame = 0


Query: 93  KILKEMLSQTRDLCWECLEIIDAAQRLGIDNHFQEEIEAVLKSQYDFMNANHF--DGDMD 152
           K +K ML   +    E LE+ID  Q+LG+  HF+ EI   L   +  M  N +  D + D
Sbjct: 83  KKVKSMLDDEKKSRLEQLELIDDLQKLGVSYHFEIEINDTLTDLHLKMGRNCWKCDKEED 142

Query: 153 LHKAALLFRLLRQ-GYLVS 169
           LH  +L FRLLRQ G+ VS
Sbjct: 143 LHATSLEFRLLRQHGFDVS 161

The following BLAST results are available for this feature:

BLAST of Lsi02G006690.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B9RXW4	2.1e-09	37.09	Probable terpene synthase 13 OS=Ricinus communis OX=3988 GN=TPS13 PE=3 SV=1	[more]
Q84UV0	7.9e-09	40.17	S-(+)-linalool synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TPS14 ...	[more]
P0CV94	3.9e-08	43.68	(3S,6E)-nerolidol synthase 1 OS=Fragaria ananassa OX=3747 PE=1 SV=1	[more]
P0CV95	6.7e-08	42.53	(3S,6E)-nerolidol synthase 2, chloroplastic/mitochondrial OS=Fragaria ananassa O...	[more]
J7LJN5	6.7e-08	45.68	Beta-caryophyllene synthase OS=Phyla dulcis OX=542674 PE=1 SV=1	[more]

BLAST of Lsi02G006690.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4E662	2.3e-51	70.59	(3S,6E)-nerolidol synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103489227 PE=4 S...	[more]
A0A0A0LX82	3.3e-50	71.97	Terpene_synth domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G0700...	[more]
A0A5D3BI68	5.7e-18	59.18	(3S,6E)-nerolidol synthase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3B3I5	5.7e-18	48.89	(3S,6E)-nerolidol synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103485759 PE=4 S...	[more]
A0A0A0LUC8	9.7e-18	61.80	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G068570 PE=4 SV=1	[more]

BLAST of Lsi02G006690.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893983.1	2.1e-59	77.11	(3S,6E)-nerolidol synthase 1-like isoform X2 [Benincasa hispida]	[more]
XP_038893982.1	2.1e-59	77.11	(3S,6E)-nerolidol synthase 1-like isoform X1 [Benincasa hispida]	[more]
XP_016903707.1	4.7e-51	70.59	PREDICTED: (3S,6E)-nerolidol synthase 1-like [Cucumis melo]	[more]
XP_011652894.1	6.8e-50	71.97	myrcene synthase, chloroplastic isoform X1 [Cucumis sativus]	[more]
XP_011652895.1	6.8e-50	71.97	probable terpene synthase 13 isoform X2 [Cucumis sativus]	[more]

BLAST of Lsi02G006690.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G61680.1	5.6e-10	40.17	terpene synthase 14	[more]
AT1G61680.2	6.2e-09	42.57	terpene synthase 14	[more]
AT2G24210.1	7.6e-07	35.78	terpene synthase 10	[more]
AT3G25810.1	4.9e-06	40.66	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT3G25820.1	1.1e-05	41.77	terpene synthase-like sequence-1,8-cineole	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036965	Terpene synthase, N-terminal domain superfamily	GENE3D	1.50.10.130		coord: 68..178 e-value: 7.6E-19 score: 69.8
IPR001906	Terpene synthase, N-terminal domain	PFAM	PF01397	Terpene_synth	coord: 94..169 e-value: 4.0E-16 score: 59.3
None	No IPR available	PANTHER	PTHR31225:SF148	(3S,6E)-NEROLIDOL SYNTHASE 2, CHLOROPLASTIC/MITOCHONDRIAL-LIKE	coord: 77..171
None	No IPR available	PANTHER	PTHR31225	OS04G0344100 PROTEIN-RELATED	coord: 77..171
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	SUPERFAMILY	48239	Terpenoid cyclases/Protein prenyltransferases	coord: 94..169

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi02G006690	Lsi02G006690	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi02G006690.1.exon.4	Lsi02G006690.1.exon.4	exon
Lsi02G006690.1.exon.3	Lsi02G006690.1.exon.3	exon
Lsi02G006690.1.exon.2	Lsi02G006690.1.exon.2	exon
Lsi02G006690.1.exon.1	Lsi02G006690.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi02G006690.1.CDS.4	Lsi02G006690.1.CDS.4	CDS
Lsi02G006690.1.CDS.3	Lsi02G006690.1.CDS.3	CDS
Lsi02G006690.1.CDS.2	Lsi02G006690.1.CDS.2	CDS
Lsi02G006690.1.CDS.1	Lsi02G006690.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi02G006690.1	Lsi02G006690.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044237	cellular metabolic process
biological_process	GO:0071704	organic substance metabolic process
biological_process	GO:0044238	primary metabolic process
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0010333	terpene synthase activity
molecular_function	GO:0000287	magnesium ion binding