Lcy13g016240.1 (mRNA) Sponge gourd (P93075) v1

Overview


Name	Lcy13g016240.1
Type	mRNA
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Eukaryotic aspartyl protease family protein
Location	Chr13: 45125175 .. 45126490 (-)
Sequence length	1254
RNA-Seq Expression	Lcy13g016240.1
Synteny	Lcy13g016240.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAATTTGTATCCTCTTCAAACTTCGATTTCCTCTTTCTTGGTACTGCTCTTGCAAATTCTCTCTTCCGAGCCAGCTCTCAGTGAATGCTCACTGAAACATGGGGTCCGGGTTGATCTTACACACTTCGACGCTGAGGAAAATTTCACCAAATTTGAAATGCTCCGACGAGGAATCGAGCGCGACAAGACCCGAATACAAAATTTCAATAAGACGACCATAAGTAACTTCGATGTTGAAATTCCTGTTGTTGAGAGAAAGGGAAGCTATCTTACGAACTTGTCGCTCGGGACGCCTCCAGTTTCGTTCTCTGCCATTGCCGATACTGGCAGCGACTTGATTTGGACGAGTGCAAGCCATGCTTGCAATGCTTTTAGCAGCCAAGCCCTGTGTATGATCCTACACGATCGTCTTCCTTCGCCAATGCCACTTCCTCTGTTGCATTGTGCAAAGCTTTGACCAATTCTAGTTCCAGCAGTGGTTGTGAGTATTCGTTTTGTTATGGAGACGGGTCTTTGACCAAAGGTTATTTAGCCTTCGAGACTTTGACGCTCGGAGAAGCAAACCAGCAAGCTTCTGCACCAGACATAGCTTTCGGGTGCGGGCTAAGGAACCATGTAAAGGGCCTGAAACAGGGTGCTGGCATGGTGGGATTCAGTCGAGGACCATTGTCGCTTATTTCACAGCTCGACATTCACAAATTTTCTTACTGTTTAACTCCAAATGGAACTGGGAGTCTGAACTTAGAACCTTCAACAAGTATCAACAACACTACGAATCGAGCCATCAAGGTCACACCGCTGATACAAAATCCATTTGACCCATCTTTCTACTATCTATCCCTACAAGGAATCACAGTAGGCCAGAAGTTCTTGCCCATCCCGTCCTCGTGGTTTAGACTAAAGACCGATAGCAACGGCGGCGTGATTATTGATTCTGGCACATCGATCACCTACTTAACAGAGGATGCATTTGATGTTCTGAAACCATTTTTCATTGCCCAGACAAACCTCCCAGTGAAACACTCAGCAGGCATTGGTCTTGATCTCTACTTTGAGATGCCTTCCCACAACAAAAGAAAACTTGGTGTGCCTAATTTGGTATTTCATTTCGAGGGGCTCGACTTGAAGCTTCCAATCGAGAACTATATGGTCACCAATGAGGAGCAGGTGTTGGTGTGTTTGGCAATGGGAGCTGCAGGAGCTTTGTCAATTTTCGATAGCATGCAACACCAGAATATGTTGGTTCTTCATGATCTCAAGAAGGAAGTCGTATCATTTGTTCCCACACAGTGTGCTCGGCTCGGTAACTAG

mRNA sequence

ATGGCGAATTTGTATCCTCTTCAAACTTCGATTTCCTCTTTCTTGGTACTGCTCTTGCAAATTCTCTCTTCCGAGCCAGCTCTCAGTGAATGCTCACTGAAACATGGGGTCCGGGTTGATCTTACACACTTCGACGCTGAGGAAAATTTCACCAAATTTGAAATGCTCCGACGAGGAATCGAGCGCGACAAGACCCGAATACAAAATTTCAATAAGACGACCATAAGTAACTTCGATGTTGAAATTCCTGTTGTTGAGAGAAAGGGAAGCTATCTTACGAACTTGTCGCTCGGGACGCCTCCAGTTTCGTTCTCTGCCATTGCCGATACTGGCAGCGACTTGATTTGGACGAGTGCAAGCCATGCTTGCAATGCTTTTAGCAGCCAAGCCCTGTCTTTGACCAATTCTAGTTCCAGCAGTGGTTGTGAGTATTCGTTTTGTTATGGAGACGGGTCTTTGACCAAAGGTTATTTAGCCTTCGAGACTTTGACGCTCGGAGAAGCAAACCAGCAAGCTTCTGCACCAGACATAGCTTTCGGGTGCGGGCTAAGGAACCATGTAAAGGGCCTGAAACAGGGTGCTGGCATGGTGGGATTCAGTCGAGGACCATTGTCGCTTATTTCACAGCTCGACATTCACAAATTTTCTTACTGTTTAACTCCAAATGGAACTGGGAGTCTGAACTTAGAACCTTCAACAAGTATCAACAACACTACGAATCGAGCCATCAAGGTCACACCGCTGATACAAAATCCATTTGACCCATCTTTCTACTATCTATCCCTACAAGGAATCACAGTAGGCCAGAAGTTCTTGCCCATCCCGTCCTCGTGGTTTAGACTAAAGACCGATAGCAACGGCGGCGTGATTATTGATTCTGGCACATCGATCACCTACTTAACAGAGGATGCATTTGATGTTCTGAAACCATTTTTCATTGCCCAGACAAACCTCCCAGTGAAACACTCAGCAGGCATTGGTCTTGATCTCTACTTTGAGATGCCTTCCCACAACAAAAGAAAACTTGGTGTGCCTAATTTGGTATTTCATTTCGAGGGGCTCGACTTGAAGCTTCCAATCGAGAACTATATGGTCACCAATGAGGAGCAGGTGTTGGTGTGTTTGGCAATGGGAGCTGCAGGAGCTTTGTCAATTTTCGATAGCATGCAACACCAGAATATGTTGGTTCTTCATGATCTCAAGAAGGAAGTCGTATCATTTGTTCCCACACAGTGTGCTCGGCTCGGTAACTAG

Coding sequence (CDS)

ATGGCGAATTTGTATCCTCTTCAAACTTCGATTTCCTCTTTCTTGGTACTGCTCTTGCAAATTCTCTCTTCCGAGCCAGCTCTCAGTGAATGCTCACTGAAACATGGGGTCCGGGTTGATCTTACACACTTCGACGCTGAGGAAAATTTCACCAAATTTGAAATGCTCCGACGAGGAATCGAGCGCGACAAGACCCGAATACAAAATTTCAATAAGACGACCATAAGTAACTTCGATGTTGAAATTCCTGTTGTTGAGAGAAAGGGAAGCTATCTTACGAACTTGTCGCTCGGGACGCCTCCAGTTTCGTTCTCTGCCATTGCCGATACTGGCAGCGACTTGATTTGGACGAGTGCAAGCCATGCTTGCAATGCTTTTAGCAGCCAAGCCCTGTCTTTGACCAATTCTAGTTCCAGCAGTGGTTGTGAGTATTCGTTTTGTTATGGAGACGGGTCTTTGACCAAAGGTTATTTAGCCTTCGAGACTTTGACGCTCGGAGAAGCAAACCAGCAAGCTTCTGCACCAGACATAGCTTTCGGGTGCGGGCTAAGGAACCATGTAAAGGGCCTGAAACAGGGTGCTGGCATGGTGGGATTCAGTCGAGGACCATTGTCGCTTATTTCACAGCTCGACATTCACAAATTTTCTTACTGTTTAACTCCAAATGGAACTGGGAGTCTGAACTTAGAACCTTCAACAAGTATCAACAACACTACGAATCGAGCCATCAAGGTCACACCGCTGATACAAAATCCATTTGACCCATCTTTCTACTATCTATCCCTACAAGGAATCACAGTAGGCCAGAAGTTCTTGCCCATCCCGTCCTCGTGGTTTAGACTAAAGACCGATAGCAACGGCGGCGTGATTATTGATTCTGGCACATCGATCACCTACTTAACAGAGGATGCATTTGATGTTCTGAAACCATTTTTCATTGCCCAGACAAACCTCCCAGTGAAACACTCAGCAGGCATTGGTCTTGATCTCTACTTTGAGATGCCTTCCCACAACAAAAGAAAACTTGGTGTGCCTAATTTGGTATTTCATTTCGAGGGGCTCGACTTGAAGCTTCCAATCGAGAACTATATGGTCACCAATGAGGAGCAGGTGTTGGTGTGTTTGGCAATGGGAGCTGCAGGAGCTTTGTCAATTTTCGATAGCATGCAACACCAGAATATGTTGGTTCTTCATGATCTCAAGAAGGAAGTCGTATCATTTGTTCCCACACAGTGTGCTCGGCTCGGTAACTAG

Protein sequence

MANLYPLQTSISSFLVLLLQILSSEPALSECSLKHGVRVDLTHFDAEENFTKFEMLRRGIERDKTRIQNFNKTTISNFDVEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSDLIWTSASHACNAFSSQALSLTNSSSSSGCEYSFCYGDGSLTKGYLAFETLTLGEANQQASAPDIAFGCGLRNHVKGLKQGAGMVGFSRGPLSLISQLDIHKFSYCLTPNGTGSLNLEPSTSINNTTNRAIKVTPLIQNPFDPSFYYLSLQGITVGQKFLPIPSSWFRLKTDSNGGVIIDSGTSITYLTEDAFDVLKPFFIAQTNLPVKHSAGIGLDLYFEMPSHNKRKLGVPNLVFHFEGLDLKLPIENYMVTNEEQVLVCLAMGAAGALSIFDSMQHQNMLVLHDLKKEVVSFVPTQCARLGN

Homology

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BLAST of Lcy13g016240.1 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 9.3e-77
Identity = 179/441 (40.59%), Postives = 249/441 (56.46%), Query Frame = 0


Query: 7   LQTSISSFLVLLLQI---LSSEPALSECSLKH-------GVRVDLTHFDAEENFTKFEML 66
           + +S+ SFL+ L  +   ++   + S  +L H       G ++ L H D+ +N TKF++L
Sbjct: 1   MASSLYSFLLALSIVYIFVAPTHSTSRTALNHRHEAKVTGFQIMLEHVDSGKNLTKFQLL 60

Query: 67  RRGIERDKTRIQNFNKTTISNFDVEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSDLIW 126
            R IER   R+Q           VE  V    G YL NLS+GTP   FSAI DTGSDLIW
Sbjct: 61  ERAIERGSRRLQRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIW 120

Query: 127 TSASHACNAFSS-----------------------QALSLTNSSSSSGCEYSFCYGDGSL 186
           T        F+                        QALS + + S++ C+Y++ YGDGS 
Sbjct: 121 TQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQALS-SPTCSNNFCQYTYGYGDGSE 180

Query: 187 TKGYLAFETLTLGEANQQASAPDIAFGCGLRNHVKGLKQGAGMVGFSRGPLSLISQLDIH 246
           T+G +  ETLT G      S P+I FGCG  N   G   GAG+VG  RGPLSL SQLD+ 
Sbjct: 181 TQGSMGTETLTFG----SVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVT 240

Query: 247 KFSYCLTPNGTGSLNLEPSTSINNTTNRAIKVTPLIQNPFDPSFYYLSLQGITVGQKFLP 306
           KFSYC+TP G+ + +     S+ N+       T LIQ+   P+FYY++L G++VG   LP
Sbjct: 241 KFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLP 300

Query: 307 IPSSWFRLKTDS-NGGVIIDSGTSITYLTEDAFDVLKPFFIAQTNLPVKHSAGIGLDLYF 366
           I  S F L +++  GG+IIDSGT++TY   +A+  ++  FI+Q NLPV + +  G DL F
Sbjct: 301 IDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCF 360

Query: 367 EMPSHNKRKLGVPNLVFHFEGLDLKLPIENYMVTNEEQVLVCLAMGAAG-ALSIFDSMQH 413
           + PS +   L +P  V HF+G DL+LP ENY + +    L+CLAMG++   +SIF ++Q 
Sbjct: 361 QTPS-DPSNLQIPTFVMHFDGGDLELPSENYFI-SPSNGLICLAMGSSSQGMSIFGNIQQ 420

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BLAST of Lcy13g016240.1 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 6.6e-75
Identity = 165/401 (41.15%), Postives = 229/401 (57.11%), Query Frame = 0


Query: 36  GVRVDLTHFDAEENFTKFEMLRRGIERDKTRIQNFNKTTISNFDVEIPVVERKGSYLTNL 95
           G+RVDL   D+ +N TK+E+++R I+R + R+++ N    S+  +E PV    G YL N+
Sbjct: 41  GLRVDLEQVDSGKNLTKYELIKRAIKRGERRMRSINAMLQSSSGIETPVYAGDGEYLMNV 100

Query: 96  SLGTPPVSFSAIADTGSDLIWTSASHACNAFSSQALSLTNSSSSS--------------- 155
           ++GTP  SFSAI DTGSDLIWT        FS          SSS               
Sbjct: 101 AIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLP 160

Query: 156 -------GCEYSFCYGDGSLTKGYLAFETLTLGEANQQASAPDIAFGCGLRNHVKGLKQG 215
                   C+Y++ YGDGS T+GY+A ET T     + +S P+IAFGCG  N   G   G
Sbjct: 161 SETCNNNECQYTYGYGDGSTTQGYMATETFTF----ETSSVPNIAFGCGEDNQGFGQGNG 220

Query: 216 AGMVGFSRGPLSLISQLDIHKFSYCLTPNGTGSLNLEPSTSINNTTNRAIKVTPLIQNPF 275
           AG++G   GPLSL SQL + +FSYC+T  G+ S +     S  +        T LI +  
Sbjct: 221 AGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSL 280

Query: 276 DPSFYYLSLQGITVGQKFLPIPSSWFRLKTDSNGGVIIDSGTSITYLTEDAFDVLKPFFI 335
           +P++YY++LQGITVG   L IPSS F+L+ D  GG+IIDSGT++TYL +DA++ +   F 
Sbjct: 281 NPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFT 340

Query: 336 AQTNLPVKHSAGIGLDLYFEMPSHNKRKLGVPNLVFHFEGLDLKLPIENYMVTNEEQVLV 395
            Q NLP    +  GL   F+ PS     + VP +   F+G  L L  +N +++  E V +
Sbjct: 341 DQINLPTVDESSSGLSTCFQQPSDGS-TVQVPEISMQFDGGVLNLGEQNILISPAEGV-I 400

Query: 396 CLAMGAAGAL--SIFDSMQHQNMLVLHDLKKEVVSFVPTQC 413
           CLAMG++  L  SIF ++Q Q   VL+DL+   VSFVPTQC
Sbjct: 401 CLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

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BLAST of Lcy13g016240.1 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 8.2e-49
Identity = 148/442 (33.48%), Postives = 210/442 (47.51%), Query Frame = 0


Query: 16  VLLLQILSSEPALSECSLKH--GVRVDLTHFDAEEN------FTKFEMLRRGIERDKTRI 75
           VLL   L S   LS  + K   G   DL H D+ ++       T  + LR  I R   R+
Sbjct: 8   VLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNRV 67

Query: 76  QNFNKTTISNFDVEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSDLIWTSASHACNAF- 135
            +F +   +    +I +    G YL N+S+GTPP    AIADTGSDL+WT  +   + + 
Sbjct: 68  FHFTEKD-NTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYT 127

Query: 136 --------------------SSQALSLTNSSSSS----GCEYSFCYGDGSLTKGYLAFET 195
                               SSQ  +L N +S S     C YS  YGD S TKG +A +T
Sbjct: 128 QVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDT 187

Query: 196 LTLGEAN-QQASAPDIAFGCGLRNHVKGLKQGAGMVGFSRGPLSLISQLDIH---KFSYC 255
           LTLG ++ +     +I  GCG  N     K+G+G+VG   GP+SLI QL      KFSYC
Sbjct: 188 LTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDGKFSYC 247

Query: 256 LTP-----NGTGSLNLEPSTSINNTTNRAIKVTPLIQNPFDPSFYYLSLQGITVGQKFLP 315
           L P     + T  +N   +  ++ +    +  TPLI      +FYYL+L+ I+VG K + 
Sbjct: 248 LVPLTSKKDQTSKINFGTNAIVSGS---GVVSTPLIAKASQETFYYLTLKSISVGSKQIQ 307

Query: 316 IPSSWFRLKTDSNGGVIIDSGTSITYLTEDAFDVLKPFFIAQTNLPVKHSAGIGLDLYFE 375
              S       S G +IIDSGT++T L  + +  L+    +  +   K     GL L + 
Sbjct: 308 YSGS---DSESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLCYS 367

Query: 376 MPSHNKRKLGVPNLVFHFEGLDLKLPIENYMVTNEEQVLVCLAMGAAGALSIFDSMQHQN 416
                   L VP +  HF+G D+KL   N  V   E  LVC A   + + SI+ ++   N
Sbjct: 368 ATG----DLKVPVITMHFDGADVKLDSSNAFVQVSED-LVCFAFRGSPSFSIYGNVAQMN 427

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BLAST of Lcy13g016240.1 vs. ExPASy Swiss-Prot
Match: Q7XV21 (Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2)

HSP 1 Score: 179.5 bits (454), Expect = 7.9e-44
Identity = 141/471 (29.94%), Postives = 222/471 (47.13%), Query Frame = 0


Query: 11  ISSFLVLLLQILSSEPALSECSLKHGVRVDLTHFDAE----ENFTKFEMLRRGIERDKTR 70
           +++ ++LLL  L++ PA   C+     R++L   DA      N T+ E+LRR I+R + R
Sbjct: 1   MNAAVLLLLLALAALPA--SCAPPRSFRLELASVDASAADAANLTEHELLRRAIQRSRYR 60

Query: 71  IQNFN------KTTISNFDVEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSDLIWTSAS 130
           +           +       E P++   G YL  L +GTPP  F+A  DT SDLIWT   
Sbjct: 61  LAGIGMARGEAASARKAVVAETPIMPAGGEYLVKLGIGTPPYKFTAAIDTASDLIWTQCQ 120

Query: 131 HACNAF-----------SSQALSLTNSSSS--------------SGCEYSFCYGDGSLTK 190
                +           SS   +L  SS +                C+Y++ Y   + T+
Sbjct: 121 PCTGCYHQVDPMFNPRVSSTYAALPCSSDTCDELDVHRCGHDDDESCQYTYTYSGNATTE 180

Query: 191 GYLAFETLTLGEANQQASAPDIAFGCGLRNHVKG-LKQGAGMVGFSRGPLSLISQLDIHK 250
           G LA + L +GE     +   +AFGC   +       Q +G+VG  RGPLSL+SQL + +
Sbjct: 181 GTLAVDKLVIGE----DAFRGVAFGCSTSSTGGAPPPQASGVVGLGRGPLSLVSQLSVRR 240

Query: 251 FSYCLTPNGT---GSLNL-EPSTSINNTTNRAIKVTPLIQNPFDPSFYYLSLQGITVGQK 310
           F+YCL P  +   G L L   + +  N TNR     P+ ++P  PS+YYL+L G+ +G +
Sbjct: 241 FAYCLPPPASRIPGKLVLGADADAARNATNRI--AVPMRRDPRYPSYYYLNLDGLLIGDR 300

Query: 311 FLPIPSSWFRLKT----------------------DSNG-GVIIDSGTSITYLTEDAFDV 370
            + +P +     T                      D+N  G+IID  ++IT+L    +D 
Sbjct: 301 AMSLPPTTTTTATATATAPAPAPTPSPNATAVAVGDANRYGMIIDIASTITFLEASLYDE 360

Query: 371 LKPFFIAQTNLPVKHSAGIGLDLYFEMPSHNK-RKLGVPNLVFHFEGLDLKLPIENYMVT 416
           L      +  LP    + +GLDL F +P      ++ VP +   F+G  L+L        
Sbjct: 361 LVNDLEVEIRLPRGTGSSLGLDLCFILPDGVAFDRVYVPAVALAFDGRWLRLDKARLFAE 420

[Close]

BLAST of Lcy13g016240.1 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 8.2e-41
Identity = 138/439 (31.44%), Postives = 201/439 (45.79%), Query Frame = 0


Query: 29  SECSLKHGVRVDLTHFDA-EENFTKFEMLRRGIERDKTRIQNFNKTTISNFDVEIP---- 88
           S+      + ++L H DA   N T  E+    ++RD  R++     +I+    +IP    
Sbjct: 64  SDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVK-----SIATLAAQIPGRNV 123

Query: 89  ----------------VVERKGSYLTNLSLGTPPVSFSAIADTGSDLIWTSASHACNAFS 148
                           + +  G Y T L +GTP      + DTGSD++W   +     +S
Sbjct: 124 THAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYS 183

Query: 149 ----------SQALSLTNSSS-------SSGCE-------YSFCYGDGSLTKGYLAFETL 208
                     S+  +    SS       S+GC        Y   YGDGS T G  + ETL
Sbjct: 184 QSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETL 243

Query: 209 TLGEANQQASAPDIAFGCGLRNHVKGLKQG-AGMVGFSRGPLSLISQLD---IHKFSYCL 268
           T     ++     +A GCG  N  +GL  G AG++G  +G LS   Q       KFSYCL
Sbjct: 244 TF----RRNRVKGVALGCGHDN--EGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCL 303

Query: 269 TPNGTGSLNLEPSTSI--NNTTNRAIKVTPLIQNPFDPSFYYLSLQGITVGQKFLP-IPS 328
                 S   +PS+ +  N   +R  + TPL+ NP   +FYY+ L GI+VG   +P + +
Sbjct: 304 VDRSASS---KPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTA 363

Query: 329 SWFRLKTDSNGGVIIDSGTSITYLTEDAFDVLKPFFIAQTNLPVKHSAGIGL-DLYFEMP 388
           S F+L    NGGVIIDSGTS+T L   A+  ++  F       +K +    L D  F++ 
Sbjct: 364 SLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAK-TLKRAPDFSLFDTCFDLS 423

Query: 389 SHNKRKLGVPNLVFHFEGLDLKLPIENYMVTNEEQVLVCLAM-GAAGALSIFDSMQHQNM 414
           + N+ K  VP +V HF G D+ LP  NY++  +     C A  G  G LSI  ++Q Q  
Sbjct: 424 NMNEVK--VPTVVLHFRGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGF 483

[Close]

BLAST of Lcy13g016240.1 vs. ExPASy TrEMBL
Match: A0A2N9HF56 (Peptidase A1 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS40859 PE=3 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 2.5e-101
Identity = 219/440 (49.77%), Postives = 280/440 (63.64%), Query Frame = 0


Query: 6   PLQTSISSFLVLLLQILSSEPAL--SECSLKHGVRVDLTHFDA-EENFTKFEMLRRG-IE 65
           P Q S+   LV++L +LS    L   E   K G RV LT  DA ++N T+ ++++R  I 
Sbjct: 7   PFQFSL-LLLVVILSLLSFSSGLPFDESPEKTGFRVSLTRVDAGKKNLTELQLVQRAIIN 66

Query: 66  RDKTRIQNFNKTT--ISNFDVEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSDLIWTSA 125
           R KTR+   +  T    N D++ P+    G +L +LS+GTP V F AI DTGSDLIWT  
Sbjct: 67  RVKTRLDRLSAITKNAPNDDIQAPIYVGDGEFLMHLSIGTPAVPFLAIMDTGSDLIWTQC 126

Query: 126 SHACNAFSSQ------ALS---------------LTNSSSSSGCEYSFCYGDGSLTKGYL 185
               N F+        ALS               L+NS   +GCEY   YGD S ++G+L
Sbjct: 127 KPCSNCFNQSTPVFEPALSSSFSNVTCSSELCKALSNSRCGNGCEYLNRYGDESSSEGFL 186

Query: 186 AFETLTLGEANQQASAPDIAFGCGLRNHVKGLKQGAGMVGFSRGPLSLISQLDIHKFSYC 245
           A ETLT GE+ Q+ S P+I FGCG+ N  KG  + AG+VG  RGPLSL+SQL   KFSYC
Sbjct: 187 AMETLTFGESAQRVSVPNIGFGCGVDNKGKGWNKAAGLVGLGRGPLSLVSQLGSKKFSYC 246

Query: 246 LT---PNGTGSLNLEPSTSINNTTNRAIKVTPLIQNPFDPSFYYLSLQGITVGQKFLPIP 305
           LT    N + SL       +N +    +K TPLI+NP  PSFYYLSL+GITVGQ  LPIP
Sbjct: 247 LTSIGDNKSSSLLFGSLADLNLSNGAVLKTTPLIRNPILPSFYYLSLEGITVGQTLLPIP 306

Query: 306 SSWFRLKTDSNGGVIIDSGTSITYLTEDAFDVLKPFFIAQTNLPVKHSAGIGLDLYFEMP 365
            S F+L +D  GGVI+DSGT+IT L EDAFDVLK  F AQT L V +    GLD+ F++P
Sbjct: 307 KSLFQLGSDGGGGVILDSGTTITSLHEDAFDVLKQVFKAQTKLRVSNLYTRGLDVCFDLP 366

Query: 366 SHNKRKLGVPNLVFHFEGLDLKLPIENYMVTNEEQVLVCLAMGAAGALSIFDSMQHQNML 416
           S + + + VP LVFHFEGLDL+LP+ENYM+ + +  +VCLAMGA G LSIF ++Q QNML
Sbjct: 367 SQDVKDIKVPKLVFHFEGLDLELPVENYMIADTKLGVVCLAMGATGRLSIFGNIQQQNML 426

[Close]

BLAST of Lcy13g016240.1 vs. ExPASy TrEMBL
Match: A0A5J5B830 (Peptidase A1 domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_028143 PE=3 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 4.7e-100
Identity = 208/424 (49.06%), Postives = 268/424 (63.21%), Query Frame = 0


Query: 7   LQTSISSFLVLLLQILSSEPALSECS--------LKHGVRVDLTHFDAEENFTKFEMLRR 66
           L  SI S L LLL   +  P  S  S        + +G RV L H  + +NFTK E++++
Sbjct: 8   LPFSILSLLFLLLTTETLLPLASSTSRRALHNHHIPNGFRVSLKHVHSGQNFTKLELIQQ 67

Query: 67  GIERDKTRIQNFNKTTI---SNFDVEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSDLI 126
            I R K R Q F        +  D++ PV    G +L N+S+GTP + FSAI DTGSDLI
Sbjct: 68  SINRSKNRTQRFETIATAKPTGDDIQTPVYAGDGEFLMNISIGTPAIPFSAIMDTGSDLI 127

Query: 127 WTSASHACNAFSSQALSLTNSSSSSGCEYSFCYGDGSLTKGYLAFETLT-LGEANQQASA 186
           WT  S                 +S+GC+Y + YGD S T+G++A ET T L    Q+ + 
Sbjct: 128 WTQCS---------------PCTSNGCKYMYMYGDESSTQGFMAMETFTFLDSVQQEVAI 187

Query: 187 PDIAFGCGLRNHVKGLKQGAGMVGFSRGPLSLISQLDIHKFSYCLTPNGTG--SLNLEPS 246
           P+I FGCGL N   GL QG+G+VG  RG LSL+SQL + KFSYCLT  G    S  L  S
Sbjct: 188 PNIGFGCGLNNQGSGLGQGSGLVGLGRGELSLVSQLGVPKFSYCLTSIGENKTSTLLLGS 247

Query: 247 TSINNTTNRAIKVTPLIQNPFDPSFYYLSLQGITVGQKFLPIPSSWFRLKTDSNGGVIID 306
            +  N +   I  TP+IQNPF PSFYY+SL+GITVG+  LPI  S FRL+ D +GG+IID
Sbjct: 248 LADLNYSGGGIHGTPIIQNPFQPSFYYISLEGITVGETLLPISKSLFRLREDGSGGMIID 307

Query: 307 SGTSITYLTEDAFDVLKPFFIAQTNLPVKHSA-GIGLDLYFEMPSHNKRKLGVPNLVFHF 366
           SGT+ITY+ EDAFDVLK  F++Q  L V  SA   GLDL F++P+ +   + VP L FHF
Sbjct: 308 SGTTITYIQEDAFDVLKKEFMSQMKLRVSRSADATGLDLCFDLPTKDVTDIAVPKLKFHF 367

Query: 367 EGLDLKLPIENYMVTNEEQVLVCLAMGAAGALSIFDSMQHQNMLVLHDLKKEVVSFVPTQ 416
           +G+DL+LP+ENYM+ +E   LVCLAM A GA+SIF ++Q QN+LVLHDL KE +SFVPTQ
Sbjct: 368 KGVDLELPVENYMIADENMKLVCLAMAATGAMSIFGNIQQQNLLVLHDLGKETLSFVPTQ 416

[Close]

BLAST of Lcy13g016240.1 vs. ExPASy TrEMBL
Match: A0A438KMS9 (Aspartic proteinase nepenthesin-1 OS=Vitis vinifera OX=29760 GN=nep1_0 PE=3 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 8.1e-100
Identity = 212/445 (47.64%), Postives = 275/445 (61.80%), Query Frame = 0


Query: 5   YPLQTSISSFLVLLLQIL-------SSEPALSECSLKHGVRVDLTHFDAEENFTKFEMLR 64
           YPL +    FL+L+L +L       +  P      L+ G +V L H DA  NFTK ++++
Sbjct: 6   YPLPS--LPFLLLILTVLGLPRSSSTLMPGFRRQQLETGFQVGLRHIDAGRNFTKLQLIQ 65

Query: 65  RGIERDKTRIQNFN--KTTISNFDVEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSDLI 124
           RGI R + R+Q  +   TT    D + PV    G ++ NL +GTPPV F AI DTGSDLI
Sbjct: 66  RGINRGRQRLQRMSGMATTAERNDFQPPVHVGDGEFVVNLMIGTPPVPFPAIMDTGSDLI 125

Query: 125 WTSASHACNAFSSQALSLTNSSSSS----------------------GCEYSFCYGDGSL 184
           WT   + C     Q+  + N   SS                       CEY + YGD S 
Sbjct: 126 WTQC-NPCKLCFQQSTPVFNPKRSSTFSNISCSSKLCKGVKPSKCDKSCEYRYTYGDESS 185

Query: 185 TKGYLAFETLTLGEANQQASAPDIAFGCGLRNHVKGLKQGAGMVGFSRGPLSLISQLDIH 244
           T+G++A +T+T GE+ ++ S P I FGCG+ N   G+ Q AG++G  RG LSL+SQL   
Sbjct: 186 TEGFMAMDTITFGESPKRVSIPRIGFGCGVNNRATGMDQTAGLLGLGRGVLSLVSQLGTQ 245

Query: 245 KFSYCLT---PNGTGSLNLEPSTSINNTTNRAIKVTPLIQNPFDPSFYYLSLQGITVGQK 304
           KFSYCLT    N T SL L  S + +N     I  TPLIQNPF PS+YYLSL+GITVG  
Sbjct: 246 KFSYCLTSIHENKTSSL-LFGSLAYSNFNPGKIPRTPLIQNPFLPSYYYLSLKGITVGYT 305

Query: 305 FLPIPSSWFRLKTDSNGGVIIDSGTSITYLTEDAFDVLKPFFIAQTNLPVKHSAGIGLDL 364
            LPIP   F+L  D +GG+I+DSGT+ITYL EDAFDVLK  FI+QT L V +S+  GLDL
Sbjct: 306 LLPIPEFAFQLGKDGSGGMILDSGTTITYLQEDAFDVLKNAFISQTELQVANSSTTGLDL 365

Query: 365 YFEMPSHNKRKLGVPNLVFHFEGLDLKLPIENYMVTNEEQVLVCLAMGAAGALSIFDSMQ 416
            F +P  N  ++ VP L+FHF+GLDL LP+ENYMV++ E  L+CLA+ A G+LSIF ++Q
Sbjct: 366 CFHLPVKNAAEVKVPKLIFHFKGLDLALPVENYMVSDPEMGLICLAIDATGSLSIFGNIQ 425

[Close]

BLAST of Lcy13g016240.1 vs. ExPASy TrEMBL
Match: F6H0S5 (Peptidase A1 domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_18s0001g03090 PE=3 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 2.6e-98
Identity = 209/445 (46.97%), Postives = 273/445 (61.35%), Query Frame = 0


Query: 5   YPLQTSISSFLVLLLQIL-------SSEPALSECSLKHGVRVDLTHFDAEENFTKFEMLR 64
           YPL +    FL+L+L +L       +  P      L+ G +V L H DA  NFT+ ++++
Sbjct: 6   YPLPS--LPFLLLILTVLGLPRSSSTLMPGFRRQQLETGFQVGLRHIDAGRNFTRLQLIQ 65

Query: 65  RGIERDKTRIQNFN--KTTISNFDVEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSDLI 124
           RGI R + R+Q  +   TT      + PV    G ++ NL +GTPPV F AI DTGSDLI
Sbjct: 66  RGINRGRQRLQRMSGMATTAERNGFQAPVHVGDGEFVVNLMIGTPPVPFPAIMDTGSDLI 125

Query: 125 WTSASHACNAFSSQALSLTNSSSSS----------------------GCEYSFCYGDGSL 184
           WT   + C     Q+  + N   SS                       CEY + YGD S 
Sbjct: 126 WTQC-NPCKLCFQQSTPVFNPKRSSTFSNISCSSKLCKGVKPSKCDKSCEYRYTYGDESS 185

Query: 185 TKGYLAFETLTLGEANQQASAPDIAFGCGLRNHVKGLKQGAGMVGFSRGPLSLISQLDIH 244
           T+G++A +T+T GE  ++ S P I FGCG+ N   G+ Q AG++G  RG LSL+SQL   
Sbjct: 186 TEGFMAMDTITFGELPKRVSIPRIGFGCGVNNRATGMDQTAGLLGLGRGVLSLVSQLGTQ 245

Query: 245 KFSYCLT---PNGTGSLNLEPSTSINNTTNRAIKVTPLIQNPFDPSFYYLSLQGITVGQK 304
           KFSYCLT    N T SL L  S + +N     I  TPLIQNPF PS+YYL+L+GITVG  
Sbjct: 246 KFSYCLTSIHENKTSSL-LFGSLAYSNFNPGKIPRTPLIQNPFLPSYYYLALKGITVGYT 305

Query: 305 FLPIPSSWFRLKTDSNGGVIIDSGTSITYLTEDAFDVLKPFFIAQTNLPVKHSAGIGLDL 364
            LPIP   F+L  D +GG+I+DSGT+ITYL EDAFDVLK  FI+QT L V +S+  GLDL
Sbjct: 306 LLPIPEFAFQLGKDGSGGMILDSGTTITYLQEDAFDVLKNAFISQTELQVANSSTTGLDL 365

Query: 365 YFEMPSHNKRKLGVPNLVFHFEGLDLKLPIENYMVTNEEQVLVCLAMGAAGALSIFDSMQ 416
            F +P  N  ++ VP L+FHF+GLDL LP+ENYMV++ E  L+CLA+ A G+LSIF ++Q
Sbjct: 366 CFHLPVKNAAEVKVPKLIFHFKGLDLALPVENYMVSDPEMGLICLAIDATGSLSIFGNIQ 425

[Close]

BLAST of Lcy13g016240.1 vs. ExPASy TrEMBL
Match: A0A6P4AXL6 (aspartic proteinase nepenthesin-1 OS=Ziziphus jujuba OX=326968 GN=LOC107425346 PE=3 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 1.1e-96
Identity = 212/447 (47.43%), Postives = 274/447 (61.30%), Query Frame = 0


Query: 15  LVLLLQILSSEPALSEC---------SLKH---GVRVDLTHFDAEENFTKFEMLRRGIER 74
           LVLL   L S PA S           S  H   G +V L H D  +N T F+ L+RGI+R
Sbjct: 19  LVLLFLALFSIPATSTSRKVVVQNHNSKNHQITGFKVSLKHVDYGKNLTSFQRLQRGIKR 78

Query: 75  DKTRIQNFN------KTTISNFD---VEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSD 134
            K R+Q  N      KT+ S+ D   VE PV    G +L  L++GTPP+++SAI DTGSD
Sbjct: 79  GKQRLQRLNAMVLADKTSSSSTDSSAVESPVNAGNGEFLMKLAIGTPPLAYSAIMDTGSD 138

Query: 135 LIWTSASHACNAFSSQ---------------------ALSLTNSSSSSGCEYSFCYGDGS 194
           LIWT  S   + FS                         +L +S+ S GCEY + YGD S
Sbjct: 139 LIWTQCSPCKDCFSQSTDIFNPKKSTSYSNLPCSSELCTALPSSTCSDGCEYYYAYGDYS 198

Query: 195 LTKGYLAFETLTLGEANQQASAPDIAFGCGLRNHVKGLKQGAGMVGFSRGPLSLISQLDI 254
            T+G LA ET T GE++ + S P+I FGCG  N   G  QGAG+VG  RGPLSL+SQL  
Sbjct: 199 STQGQLATETFTFGESDAEVSVPNIGFGCGEDNEGDGFNQGAGLVGLGRGPLSLVSQLKE 258

Query: 255 HKFSYCLT---PNGTGSLNLEPSTSIN-NTTNRAIKVTPLIQNPFDPSFYYLSLQGITVG 314
            KFSYCL     + + SL L    ++N +T++ AIK TPL+QNP  PSFYYLSLQGITVG
Sbjct: 259 PKFSYCLNSIDDSKSSSLLLGSLANLNGSTSSHAIKTTPLVQNPSQPSFYYLSLQGITVG 318

Query: 315 QKFLPIPSSWFRLKTDSNGGVIIDSGTSITYLTEDAFDVLKPFFIAQTNLPVKHSAGIGL 374
           ++ LPI    F L+ D +GG+IIDSGT+ITYL E A+D L+  F ++ NLPV  S   GL
Sbjct: 319 EERLPISKDTFSLQDDGSGGLIIDSGTTITYLEESAYDELRKVFSSKVNLPVDDSGSEGL 378

Query: 375 DLYFEMPSHNKRKLGVPNLVFHFEGLDLKLPIENYMVTNEEQVLVCLAMGAAGALSIFDS 416
           DL F++PS +  K+ VP L+FHF+G DL  P ENY++ + +  L+CLAMGA+  +SI  +
Sbjct: 379 DLCFKLPS-DSSKVDVPKLIFHFDGADLDFPPENYVLLDSDLGLLCLAMGASSGMSILGN 438

[Close]

BLAST of Lcy13g016240.1 vs. NCBI nr
Match: XP_038886455.1 (aspartic proteinase nepenthesin-2-like [Benincasa hispida])

HSP 1 Score: 567.8 bits (1462), Expect = 7.9e-158
Identity = 295/422 (69.91%), Postives = 338/422 (80.09%), Query Frame = 0


Query: 15  LVLLLQILSSEPALSECSLKHGVRVDLTHFDAEENFTKFEMLRRGIERDKTRIQNFNKTT 74
           L+LLL +LSS+PA SE SLK G   DLTH DA  NF+KFE+LRRGI+RDKT IQNFNK  
Sbjct: 10  LLLLLPLLSSKPAFSESSLKPGFHFDLTHVDATWNFSKFEILRRGIKRDKTWIQNFNKMI 69

Query: 75  ISNFDVEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSDLIWT----------------- 134
           ISN  V +PVVE++GSYL NLSLGTPP+SFSAI DTGSDL+WT                 
Sbjct: 70  ISNLKVRMPVVEQRGSYLMNLSLGTPPISFSAIFDTGSDLVWTQCKPCLQCFKQPTPVYD 129

Query: 135 --SASHACNAFSSQAL--SLTNSSSSSGCEYSFCYGDGSLTKGYLAFETLTLGEANQQAS 194
              +S   NA  S AL   LTNSSSS+GCEYSF Y D S T GYLAFETLTLG ANQ+ S
Sbjct: 130 PAQSSSFANATRSAALCKELTNSSSSNGCEYSFSYADESFTTGYLAFETLTLGGANQKVS 189

Query: 195 APDIAFGCGLRNHVKGLKQGAGMVGFSRGPLSLISQLDIHKFSYCLTPNGTGSLNLEPST 254
            PDIAFGCG++NHVKGL QGAG+VGF+RGPLSL+SQL I KFSYCL+ NGTGSL    ST
Sbjct: 190 TPDIAFGCGIKNHVKGLTQGAGIVGFNRGPLSLLSQLHIKKFSYCLSLNGTGSLVFGSST 249

Query: 255 SINNTTNRAIKVTPLIQNPFDPSFYYLSLQGITVGQKFLPIPSSWFRLKTDSNGGVIIDS 314
           SI+NTTNRAIK TPLIQNP++PS+YYLSLQGITVGQK+LP+PSS FRL TD +GG+IIDS
Sbjct: 250 SIDNTTNRAIKTTPLIQNPYNPSYYYLSLQGITVGQKYLPVPSSSFRLNTDGSGGMIIDS 309

Query: 315 GTSITYLTEDAFDVLKPFFIAQTNLPVKHSAGIGLDLYFEMPSHNKRKLGVPNLVFHFEG 374
           GTS+TY+TED FDVLKP F +QTNL V  S+ IGLDL F++PSHN  KL VP+L+FHFE 
Sbjct: 310 GTSMTYITEDVFDVLKPVFASQTNLQVIWSS-IGLDLCFQLPSHNNSKLDVPDLIFHFED 369

Query: 375 LDLKLPIENYMVTNEEQVLVCLAMGAAGALSIFDSMQHQNMLVLHDLKKEVVSFVPTQCA 416
           LDLKLP+ENYMV NEE  +VCLAMGAAG LSIF SMQHQNMLVLHDL+K+VVSF+PTQC+
Sbjct: 370 LDLKLPVENYMVVNEELGIVCLAMGAAGGLSIFGSMQHQNMLVLHDLEKKVVSFIPTQCS 429

[Close]

BLAST of Lcy13g016240.1 vs. NCBI nr
Match: KAA8538486.1 (hypothetical protein F0562_028143 [Nyssa sinensis])

HSP 1 Score: 374.8 bits (961), Expect = 9.8e-100
Identity = 208/424 (49.06%), Postives = 268/424 (63.21%), Query Frame = 0


Query: 7   LQTSISSFLVLLLQILSSEPALSECS--------LKHGVRVDLTHFDAEENFTKFEMLRR 66
           L  SI S L LLL   +  P  S  S        + +G RV L H  + +NFTK E++++
Sbjct: 8   LPFSILSLLFLLLTTETLLPLASSTSRRALHNHHIPNGFRVSLKHVHSGQNFTKLELIQQ 67

Query: 67  GIERDKTRIQNFNKTTI---SNFDVEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSDLI 126
            I R K R Q F        +  D++ PV    G +L N+S+GTP + FSAI DTGSDLI
Sbjct: 68  SINRSKNRTQRFETIATAKPTGDDIQTPVYAGDGEFLMNISIGTPAIPFSAIMDTGSDLI 127

Query: 127 WTSASHACNAFSSQALSLTNSSSSSGCEYSFCYGDGSLTKGYLAFETLT-LGEANQQASA 186
           WT  S                 +S+GC+Y + YGD S T+G++A ET T L    Q+ + 
Sbjct: 128 WTQCS---------------PCTSNGCKYMYMYGDESSTQGFMAMETFTFLDSVQQEVAI 187

Query: 187 PDIAFGCGLRNHVKGLKQGAGMVGFSRGPLSLISQLDIHKFSYCLTPNGTG--SLNLEPS 246
           P+I FGCGL N   GL QG+G+VG  RG LSL+SQL + KFSYCLT  G    S  L  S
Sbjct: 188 PNIGFGCGLNNQGSGLGQGSGLVGLGRGELSLVSQLGVPKFSYCLTSIGENKTSTLLLGS 247

Query: 247 TSINNTTNRAIKVTPLIQNPFDPSFYYLSLQGITVGQKFLPIPSSWFRLKTDSNGGVIID 306
            +  N +   I  TP+IQNPF PSFYY+SL+GITVG+  LPI  S FRL+ D +GG+IID
Sbjct: 248 LADLNYSGGGIHGTPIIQNPFQPSFYYISLEGITVGETLLPISKSLFRLREDGSGGMIID 307

Query: 307 SGTSITYLTEDAFDVLKPFFIAQTNLPVKHSA-GIGLDLYFEMPSHNKRKLGVPNLVFHF 366
           SGT+ITY+ EDAFDVLK  F++Q  L V  SA   GLDL F++P+ +   + VP L FHF
Sbjct: 308 SGTTITYIQEDAFDVLKKEFMSQMKLRVSRSADATGLDLCFDLPTKDVTDIAVPKLKFHF 367

Query: 367 EGLDLKLPIENYMVTNEEQVLVCLAMGAAGALSIFDSMQHQNMLVLHDLKKEVVSFVPTQ 416
           +G+DL+LP+ENYM+ +E   LVCLAM A GA+SIF ++Q QN+LVLHDL KE +SFVPTQ
Sbjct: 368 KGVDLELPVENYMIADENMKLVCLAMAATGAMSIFGNIQQQNLLVLHDLGKETLSFVPTQ 416

[Close]

BLAST of Lcy13g016240.1 vs. NCBI nr
Match: RVX22508.1 (Aspartic proteinase nepenthesin-1 [Vitis vinifera])

HSP 1 Score: 374.0 bits (959), Expect = 1.7e-99
Identity = 212/445 (47.64%), Postives = 275/445 (61.80%), Query Frame = 0


Query: 5   YPLQTSISSFLVLLLQIL-------SSEPALSECSLKHGVRVDLTHFDAEENFTKFEMLR 64
           YPL +    FL+L+L +L       +  P      L+ G +V L H DA  NFTK ++++
Sbjct: 6   YPLPS--LPFLLLILTVLGLPRSSSTLMPGFRRQQLETGFQVGLRHIDAGRNFTKLQLIQ 65

Query: 65  RGIERDKTRIQNFN--KTTISNFDVEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSDLI 124
           RGI R + R+Q  +   TT    D + PV    G ++ NL +GTPPV F AI DTGSDLI
Sbjct: 66  RGINRGRQRLQRMSGMATTAERNDFQPPVHVGDGEFVVNLMIGTPPVPFPAIMDTGSDLI 125

Query: 125 WTSASHACNAFSSQALSLTNSSSSS----------------------GCEYSFCYGDGSL 184
           WT   + C     Q+  + N   SS                       CEY + YGD S 
Sbjct: 126 WTQC-NPCKLCFQQSTPVFNPKRSSTFSNISCSSKLCKGVKPSKCDKSCEYRYTYGDESS 185

Query: 185 TKGYLAFETLTLGEANQQASAPDIAFGCGLRNHVKGLKQGAGMVGFSRGPLSLISQLDIH 244
           T+G++A +T+T GE+ ++ S P I FGCG+ N   G+ Q AG++G  RG LSL+SQL   
Sbjct: 186 TEGFMAMDTITFGESPKRVSIPRIGFGCGVNNRATGMDQTAGLLGLGRGVLSLVSQLGTQ 245

Query: 245 KFSYCLT---PNGTGSLNLEPSTSINNTTNRAIKVTPLIQNPFDPSFYYLSLQGITVGQK 304
           KFSYCLT    N T SL L  S + +N     I  TPLIQNPF PS+YYLSL+GITVG  
Sbjct: 246 KFSYCLTSIHENKTSSL-LFGSLAYSNFNPGKIPRTPLIQNPFLPSYYYLSLKGITVGYT 305

Query: 305 FLPIPSSWFRLKTDSNGGVIIDSGTSITYLTEDAFDVLKPFFIAQTNLPVKHSAGIGLDL 364
            LPIP   F+L  D +GG+I+DSGT+ITYL EDAFDVLK  FI+QT L V +S+  GLDL
Sbjct: 306 LLPIPEFAFQLGKDGSGGMILDSGTTITYLQEDAFDVLKNAFISQTELQVANSSTTGLDL 365

Query: 365 YFEMPSHNKRKLGVPNLVFHFEGLDLKLPIENYMVTNEEQVLVCLAMGAAGALSIFDSMQ 416
            F +P  N  ++ VP L+FHF+GLDL LP+ENYMV++ E  L+CLA+ A G+LSIF ++Q
Sbjct: 366 CFHLPVKNAAEVKVPKLIFHFKGLDLALPVENYMVSDPEMGLICLAIDATGSLSIFGNIQ 425

[Close]

BLAST of Lcy13g016240.1 vs. NCBI nr
Match: XP_034676400.1 (aspartic proteinase nepenthesin-1-like [Vitis riparia])

HSP 1 Score: 371.7 bits (953), Expect = 8.3e-99
Identity = 210/445 (47.19%), Postives = 274/445 (61.57%), Query Frame = 0


Query: 5   YPLQTSISSFLVLLLQIL-------SSEPALSECSLKHGVRVDLTHFDAEENFTKFEMLR 64
           YPL +    FL+L+L +L       +  P      L+ G +V L H DA  NFTK ++++
Sbjct: 6   YPLPS--LPFLLLILTVLGLPRSSSTLMPGFRRQQLETGFQVGLRHIDAGRNFTKLQLIQ 65

Query: 65  RGIERDKTRIQNFN--KTTISNFDVEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSDLI 124
           RGI+R + R+Q  +   TT    D + PV    G ++ NL +GTPPV F AI DTGSDLI
Sbjct: 66  RGIDRGRQRLQRMSGMATTAERNDFQAPVHAGDGEFVVNLMIGTPPVPFPAIMDTGSDLI 125

Query: 125 WTSASHACNAFSSQALSLTNSSSSS----------------------GCEYSFCYGDGSL 184
           WT   + C     Q+  + N   SS                       CEY + YGD S 
Sbjct: 126 WTQC-NPCKLCFQQSTPVFNPKRSSTFSNISCSSKLCEGVKPSKCDKSCEYRYTYGDESS 185

Query: 185 TKGYLAFETLTLGEANQQASAPDIAFGCGLRNHVKGLKQGAGMVGFSRGPLSLISQLDIH 244
           T+G++A +T+T GE  ++ S P I FGCG+ N   G+ Q AG++G  RG LSL+SQL   
Sbjct: 186 TEGFMAMDTITFGELPKRVSIPRIGFGCGVNNRATGMDQTAGLLGLGRGVLSLVSQLSTQ 245

Query: 245 KFSYCLT---PNGTGSLNLEPSTSINNTTNRAIKVTPLIQNPFDPSFYYLSLQGITVGQK 304
           KFSYCLT    N T SL L  S + +N     I  TPLIQNPF PS+YYLSL+GITVG  
Sbjct: 246 KFSYCLTSIHENKTSSL-LFGSLAYSNFNPGKIPRTPLIQNPFLPSYYYLSLKGITVGYT 305

Query: 305 FLPIPSSWFRLKTDSNGGVIIDSGTSITYLTEDAFDVLKPFFIAQTNLPVKHSAGIGLDL 364
            LPIP   F+L  D +GG+I+DSGT+ITYL EDAFDVLK  FI+QT L V +S+  GLDL
Sbjct: 306 LLPIPDFAFQLGKDGSGGMILDSGTTITYLQEDAFDVLKNAFISQTELQVANSSTTGLDL 365

Query: 365 YFEMPSHNKRKLGVPNLVFHFEGLDLKLPIENYMVTNEEQVLVCLAMGAAGALSIFDSMQ 416
            F +P  N  ++ VP L+FHF+GLDL LP+ENYMV++ +  L+CLA+ A G+ SIF ++Q
Sbjct: 366 CFHLPVKNAAEVKVPKLIFHFKGLDLALPVENYMVSDPDMGLICLAIDATGSPSIFGNIQ 425

[Close]

BLAST of Lcy13g016240.1 vs. NCBI nr
Match: XP_010664223.1 (PREDICTED: aspartic proteinase nepenthesin-1 [Vitis vinifera])

HSP 1 Score: 369.0 bits (946), Expect = 5.4e-98
Identity = 209/445 (46.97%), Postives = 273/445 (61.35%), Query Frame = 0


Query: 5   YPLQTSISSFLVLLLQIL-------SSEPALSECSLKHGVRVDLTHFDAEENFTKFEMLR 64
           YPL +    FL+L+L +L       +  P      L+ G +V L H DA  NFT+ ++++
Sbjct: 6   YPLPS--LPFLLLILTVLGLPRSSSTLMPGFRRQQLETGFQVGLRHIDAGRNFTRLQLIQ 65

Query: 65  RGIERDKTRIQNFN--KTTISNFDVEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSDLI 124
           RGI R + R+Q  +   TT      + PV    G ++ NL +GTPPV F AI DTGSDLI
Sbjct: 66  RGINRGRQRLQRMSGMATTAERNGFQAPVHVGDGEFVVNLMIGTPPVPFPAIMDTGSDLI 125

Query: 125 WTSASHACNAFSSQALSLTNSSSSS----------------------GCEYSFCYGDGSL 184
           WT   + C     Q+  + N   SS                       CEY + YGD S 
Sbjct: 126 WTQC-NPCKLCFQQSTPVFNPKRSSTFSNISCSSKLCKGVKPSKCDKSCEYRYTYGDESS 185

Query: 185 TKGYLAFETLTLGEANQQASAPDIAFGCGLRNHVKGLKQGAGMVGFSRGPLSLISQLDIH 244
           T+G++A +T+T GE  ++ S P I FGCG+ N   G+ Q AG++G  RG LSL+SQL   
Sbjct: 186 TEGFMAMDTITFGELPKRVSIPRIGFGCGVNNRATGMDQTAGLLGLGRGVLSLVSQLGTQ 245

Query: 245 KFSYCLT---PNGTGSLNLEPSTSINNTTNRAIKVTPLIQNPFDPSFYYLSLQGITVGQK 304
           KFSYCLT    N T SL L  S + +N     I  TPLIQNPF PS+YYL+L+GITVG  
Sbjct: 246 KFSYCLTSIHENKTSSL-LFGSLAYSNFNPGKIPRTPLIQNPFLPSYYYLALKGITVGYT 305

Query: 305 FLPIPSSWFRLKTDSNGGVIIDSGTSITYLTEDAFDVLKPFFIAQTNLPVKHSAGIGLDL 364
            LPIP   F+L  D +GG+I+DSGT+ITYL EDAFDVLK  FI+QT L V +S+  GLDL
Sbjct: 306 LLPIPEFAFQLGKDGSGGMILDSGTTITYLQEDAFDVLKNAFISQTELQVANSSTTGLDL 365

Query: 365 YFEMPSHNKRKLGVPNLVFHFEGLDLKLPIENYMVTNEEQVLVCLAMGAAGALSIFDSMQ 416
            F +P  N  ++ VP L+FHF+GLDL LP+ENYMV++ E  L+CLA+ A G+LSIF ++Q
Sbjct: 366 CFHLPVKNAAEVKVPKLIFHFKGLDLALPVENYMVSDPEMGLICLAIDATGSLSIFGNIQ 425

[Close]

BLAST of Lcy13g016240.1 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 309.3 bits (791), Expect = 4.7e-84
Identity = 191/452 (42.26%), Postives = 257/452 (56.86%), Query Frame = 0


Query: 14  FLVL---LLQILSSEPALSECSL-----KHGVRVDLTHFDAEENFTKFEMLRRGIERDKT 73
           FL+L   L+ + SS  +L + +L     + G R+ L H D+ +N TK + ++RGI R   
Sbjct: 14  FLILFSCLISVSSSRRSLIDRTLPKNLPRSGFRLSLRHVDSGKNLTKIQKIQRGINRGFH 73

Query: 74  RIQNFNKTTI--------SNFDVEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSDLIWT 133
           R+       +           +++ P     G +L  LS+G P V +SAI DTGSDLIWT
Sbjct: 74  RLNRLGAVAVLAVASKPDDTNNIKAPTHGGSGEFLMELSIGNPAVKYSAIVDTGSDLIWT 133

Query: 134 --------------------SASHA---CNAFSSQALSLTN-SSSSSGCEYSFCYGDGSL 193
                               S+S++   C++    AL  +N +     CEY + YGD S 
Sbjct: 134 QCKPCTECFDQPTPIFDPEKSSSYSKVGCSSGLCNALPRSNCNEDKDACEYLYTYGDYSS 193

Query: 194 TKGYLAFETLTLGEANQQASAPDIAFGCGLRNHVKGLKQGAGMVGFSRGPLSLISQLDIH 253
           T+G LA ET T  + N   S   I FGCG+ N   G  QG+G+VG  RGPLSLISQL   
Sbjct: 194 TRGLLATETFTFEDEN---SISGIGFGCGVENEGDGFSQGSGLVGLGRGPLSLISQLKET 253

Query: 254 KFSYCLT----PNGTGSLNL-EPSTSINNTTNRAI-----KVTPLIQNPFDPSFYYLSLQ 313
           KFSYCLT       + SL +   ++ I N T  ++     K   L++NP  PSFYYL LQ
Sbjct: 254 KFSYCLTSIEDSEASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQPSFYYLELQ 313

Query: 314 GITVGQKFLPIPSSWFRLKTDSNGGVIIDSGTSITYLTEDAFDVLKPFFIAQTNLPVKHS 373
           GITVG K L +  S F L  D  GG+IIDSGT+ITYL E AF VLK  F ++ +LPV  S
Sbjct: 314 GITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLPVDDS 373

Query: 374 AGIGLDLYFEMPSHNKRKLGVPNLVFHFEGLDLKLPIENYMVTNEEQVLVCLAMGAAGAL 416
              GLDL F++P   K  + VP ++FHF+G DL+LP ENYMV +    ++CLAMG++  +
Sbjct: 374 GSTGLDLCFKLPDAAK-NIAVPKMIFHFKGADLELPGENYMVADSSTGVLCLAMGSSNGM 433

[Close]

BLAST of Lcy13g016240.1 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 196.1 bits (497), Expect = 5.8e-50
Identity = 148/442 (33.48%), Postives = 210/442 (47.51%), Query Frame = 0


Query: 16  VLLLQILSSEPALSECSLKH--GVRVDLTHFDAEEN------FTKFEMLRRGIERDKTRI 75
           VLL   L S   LS  + K   G   DL H D+ ++       T  + LR  I R   R+
Sbjct: 8   VLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNRV 67

Query: 76  QNFNKTTISNFDVEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSDLIWTSASHACNAF- 135
            +F +   +    +I +    G YL N+S+GTPP    AIADTGSDL+WT  +   + + 
Sbjct: 68  FHFTEKD-NTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYT 127

Query: 136 --------------------SSQALSLTNSSSSS----GCEYSFCYGDGSLTKGYLAFET 195
                               SSQ  +L N +S S     C YS  YGD S TKG +A +T
Sbjct: 128 QVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDT 187

Query: 196 LTLGEAN-QQASAPDIAFGCGLRNHVKGLKQGAGMVGFSRGPLSLISQLDIH---KFSYC 255
           LTLG ++ +     +I  GCG  N     K+G+G+VG   GP+SLI QL      KFSYC
Sbjct: 188 LTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDGKFSYC 247

Query: 256 LTP-----NGTGSLNLEPSTSINNTTNRAIKVTPLIQNPFDPSFYYLSLQGITVGQKFLP 315
           L P     + T  +N   +  ++ +    +  TPLI      +FYYL+L+ I+VG K + 
Sbjct: 248 LVPLTSKKDQTSKINFGTNAIVSGS---GVVSTPLIAKASQETFYYLTLKSISVGSKQIQ 307

Query: 316 IPSSWFRLKTDSNGGVIIDSGTSITYLTEDAFDVLKPFFIAQTNLPVKHSAGIGLDLYFE 375
              S       S G +IIDSGT++T L  + +  L+    +  +   K     GL L + 
Sbjct: 308 YSGS---DSESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLCYS 367

Query: 376 MPSHNKRKLGVPNLVFHFEGLDLKLPIENYMVTNEEQVLVCLAMGAAGALSIFDSMQHQN 416
                   L VP +  HF+G D+KL   N  V   E  LVC A   + + SI+ ++   N
Sbjct: 368 ATG----DLKVPVITMHFDGADVKLDSSNAFVQVSED-LVCFAFRGSPSFSIYGNVAQMN 427

[Close]

BLAST of Lcy13g016240.1 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 170.6 bits (431), Expect = 2.6e-42
Identity = 143/448 (31.92%), Postives = 215/448 (47.99%), Query Frame = 0


Query: 13  SFLVLLLQILSSEPALSECSLKHGVRVDLTHFDA------EENFTKFEMLRRGIERDKTR 72
           +FL   L  +S   A +  + +  + V+L H D+        + T  + L     R  +R
Sbjct: 5   TFLYCSLLAISFFFASNSSANRENLTVELIHRDSPHSPLYNPHHTVSDRLNAAFLRSISR 64

Query: 73  IQNFNKTTISNFDVEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSDLIW---------- 132
            + F   T    D++  ++   G Y  ++S+GTPP    AIADTGSDL W          
Sbjct: 65  SRRFTTKT----DLQSGLISNGGEYFMSISIGTPPSKVFAIADTGSDLTWVQCKPCQQCY 124

Query: 133 -------------TSASHACNAFSSQALSLTN---SSSSSGCEYSFCYGDGSLTKGYLAF 192
                        T  + +C++ + QALS        S   C+Y + YGD S TKG +A 
Sbjct: 125 KQNSPLFDKKKSSTYKTESCDSKTCQALSEHEEGCDESKDICKYRYSYGDNSFTKGDVAT 184

Query: 193 ETLTL-GEANQQASAPDIAFGCGLRNHVKGLKQGAGMVGFSRGPLSLISQLDI---HKFS 252
           ET+++   +    S P   FGCG  N     + G+G++G   GPLSL+SQL      KFS
Sbjct: 185 ETISIDSSSGSSVSFPGTVFGCGYNNGGTFEETGSGIIGLGGGPLSLVSQLGSSIGKKFS 244

Query: 253 YCL-----TPNGTGSLNL-EPSTSINNTTNRAIKVTPLIQNPFDP-SFYYLSLQGITVGQ 312
           YCL     T NGT  +NL   S   N + + A   TPLIQ   DP ++Y+L+L+ +TVG+
Sbjct: 245 YCLSHTAATTNGTSVINLGTNSIPSNPSKDSATLTTPLIQK--DPETYYFLTLEAVTVGK 304

Query: 313 KFLPIPSSWFRLKTDSN---GGVIIDSGTSITYLTEDAFDVLKPFFIAQTNLPVKHSAGI 372
             LP     + L   S+   G +IIDSGT++T L    +D              + S   
Sbjct: 305 TKLPYTGGGYGLNGKSSKRTGNIIIDSGTTLTLLDSGFYDDFGTAVEESVTGAKRVSDPQ 364

Query: 373 GLDLYFEMPSHNKRKLGVPNLVFHFEGLDLKL-PIENYMVTNEEQVLVCLAMGAAGALSI 414
           GL L     S +K ++G+P +  HF   D+KL PI  ++  NE+   VCL+M     ++I
Sbjct: 365 GL-LTHCFKSGDK-EIGLPAITMHFTNADVKLSPINAFVKLNED--TVCLSMIPTTEVAI 424

[Close]

BLAST of Lcy13g016240.1 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 169.5 bits (428), Expect = 5.8e-42
Identity = 138/439 (31.44%), Postives = 201/439 (45.79%), Query Frame = 0


Query: 29  SECSLKHGVRVDLTHFDA-EENFTKFEMLRRGIERDKTRIQNFNKTTISNFDVEIP---- 88
           S+      + ++L H DA   N T  E+    ++RD  R++     +I+    +IP    
Sbjct: 64  SDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVK-----SIATLAAQIPGRNV 123

Query: 89  ----------------VVERKGSYLTNLSLGTPPVSFSAIADTGSDLIWTSASHACNAFS 148
                           + +  G Y T L +GTP      + DTGSD++W   +     +S
Sbjct: 124 THAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYS 183

Query: 149 ----------SQALSLTNSSS-------SSGCE-------YSFCYGDGSLTKGYLAFETL 208
                     S+  +    SS       S+GC        Y   YGDGS T G  + ETL
Sbjct: 184 QSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETL 243

Query: 209 TLGEANQQASAPDIAFGCGLRNHVKGLKQG-AGMVGFSRGPLSLISQLD---IHKFSYCL 268
           T     ++     +A GCG  N  +GL  G AG++G  +G LS   Q       KFSYCL
Sbjct: 244 TF----RRNRVKGVALGCGHDN--EGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCL 303

Query: 269 TPNGTGSLNLEPSTSI--NNTTNRAIKVTPLIQNPFDPSFYYLSLQGITVGQKFLP-IPS 328
                 S   +PS+ +  N   +R  + TPL+ NP   +FYY+ L GI+VG   +P + +
Sbjct: 304 VDRSASS---KPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTA 363

Query: 329 SWFRLKTDSNGGVIIDSGTSITYLTEDAFDVLKPFFIAQTNLPVKHSAGIGL-DLYFEMP 388
           S F+L    NGGVIIDSGTS+T L   A+  ++  F       +K +    L D  F++ 
Sbjct: 364 SLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAK-TLKRAPDFSLFDTCFDLS 423

Query: 389 SHNKRKLGVPNLVFHFEGLDLKLPIENYMVTNEEQVLVCLAM-GAAGALSIFDSMQHQNM 414
           + N+ K  VP +V HF G D+ LP  NY++  +     C A  G  G LSI  ++Q Q  
Sbjct: 424 NMNEVK--VPTVVLHFRGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGF 483

[Close]

BLAST of Lcy13g016240.1 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 167.5 bits (423), Expect = 2.2e-41
Identity = 134/447 (29.98%), Postives = 215/447 (48.10%), Query Frame = 0


Query: 16  VLLLQILSSEPALSECSLKHGVRVDLTHFDA------EENFTKFEMLRRGIERDKTRIQN 75
           +LL   L     LS         V+L H D+          T  + L     R  +R + 
Sbjct: 5   ILLCFFLFFSVTLSSSGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRSRR 64

Query: 76  FNKTTISNFDVEIPVVERKGSYLTNLSLGTPPVSFSAIADTGSDLIW------------- 135
           FN   +S  D++  ++   G +  ++++GTPP+   AIADTGSDL W             
Sbjct: 65  FNH-QLSQTDLQSGLIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKEN 124

Query: 136 ----------TSASHACNAFSSQALSLTN---SSSSSGCEYSFCYGDGSLTKGYLAFETL 195
                     T  S  C++ + QALS T      S++ C+Y + YGD S +KG +A ET+
Sbjct: 125 GPIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETV 184

Query: 196 TLGEAN-QQASAPDIAFGCGLRNHVKGLKQGAGMVGFSRGPLSLISQLD---IHKFSYCL 255
           ++  A+    S P   FGCG  N     + G+G++G   G LSLISQL      KFSYCL
Sbjct: 185 SIDSASGSPVSFPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQLGSSISKKFSYCL 244

Query: 256 -----TPNGTGSLNLEPSTSINN-TTNRAIKVTPLIQNPFDP-SFYYLSLQGITVGQKFL 315
                T NGT  +NL  ++  ++ + +  +  TPL+    +P ++YYL+L+ I+VG+K +
Sbjct: 245 SHKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDK--EPLTYYYLTLEAISVGKKKI 304

Query: 316 PIPSSWFR-----LKTDSNGGVIIDSGTSITYLTEDAFDVLKPFFIAQTNLPVKHSAGIG 375
           P   S +      + ++++G +IIDSGT++T L    FD              + S   G
Sbjct: 305 PYTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQG 364

Query: 376 LDLYFEMPSHNKRKLGVPNLVFHFEGLDLKL-PIENYMVTNEEQVLVCLAMGAAGALSIF 414
             L          ++G+P +  HF G D++L PI  ++  +E+  +VCL+M     ++I+
Sbjct: 365 --LLSHCFKSGSAEIGLPEITVHFTGADVRLSPINAFVKLSED--MVCLSMVPTTEVAIY 424

The following BLAST results are available for this feature:

BLAST of Lcy13g016240.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q766C3	9.3e-77	40.59	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	6.6e-75	41.15	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q6XBF8	8.2e-49	33.48	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]
Q7XV21	7.9e-44	29.94	Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2	[more]
Q9LNJ3	8.2e-41	31.44	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]

BLAST of Lcy13g016240.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2N9HF56	2.5e-101	49.77	Peptidase A1 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS4...	[more]
A0A5J5B830	4.7e-100	49.06	Peptidase A1 domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_0281...	[more]
A0A438KMS9	8.1e-100	47.64	Aspartic proteinase nepenthesin-1 OS=Vitis vinifera OX=29760 GN=nep1_0 PE=3 SV=1	[more]
F6H0S5	2.6e-98	46.97	Peptidase A1 domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_18s0001...	[more]
A0A6P4AXL6	1.1e-96	47.43	aspartic proteinase nepenthesin-1 OS=Ziziphus jujuba OX=326968 GN=LOC107425346 P...	[more]

BLAST of Lcy13g016240.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886455.1	7.9e-158	69.91	aspartic proteinase nepenthesin-2-like [Benincasa hispida]	[more]
KAA8538486.1	9.8e-100	49.06	hypothetical protein F0562_028143 [Nyssa sinensis]	[more]
RVX22508.1	1.7e-99	47.64	Aspartic proteinase nepenthesin-1 [Vitis vinifera]	[more]
XP_034676400.1	8.3e-99	47.19	aspartic proteinase nepenthesin-1-like [Vitis riparia]	[more]
XP_010664223.1	5.4e-98	46.97	PREDICTED: aspartic proteinase nepenthesin-1 [Vitis vinifera]	[more]

BLAST of Lcy13g016240.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G03200.1	4.7e-84	42.26	Eukaryotic aspartyl protease family protein	[more]
AT5G33340.1	5.8e-50	33.48	Eukaryotic aspartyl protease family protein	[more]
AT1G31450.1	2.6e-42	31.92	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	5.8e-42	31.44	Eukaryotic aspartyl protease family protein	[more]
AT2G35615.1	2.2e-41	29.98	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 289..300 score: 43.68 coord: 97..117 score: 43.43
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 120..228 e-value: 2.9E-20 score: 73.2
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 232..416 e-value: 3.2E-44 score: 152.8
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 73..229 e-value: 5.5E-34 score: 119.8
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 87..415
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 257..408 e-value: 2.2E-25 score: 89.3
None	No IPR available	PANTHER	PTHR47967:SF23	OS08G0469000 PROTEIN	coord: 20..414
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 20..414
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 289..300
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 106..117
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 91..408 score: 32.560482
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 91..412 e-value: 2.21256E-94 score: 282.615

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lcy13g016240	Lcy13g016240	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy13g016240.1.exon2	Lcy13g016240.1.exon2	exon
Lcy13g016240.1.exon1	Lcy13g016240.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy13g016240.1.cds2	Lcy13g016240.1.cds2	CDS
Lcy13g016240.1.cds1	Lcy13g016240.1.cds1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lcy13g016240.1	Lcy13g016240.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity