Lcy12g019760.1 (mRNA) Sponge gourd (P93075) v1

BLAST of Lcy12g019760.1 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 1.6e-83
Identity = 176/478 (36.82%), Postives = 254/478 (53.14%), Query Frame = 0

Query: 55  NRWLIFISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSK-------LPDEN 114
           +RW+IF+S++++  +     P+     ++    N  S NG  LGLL         +P   
Sbjct: 85  HRWVIFVSIIVRKLIAIFGKPMEWTGYLVEFFLNLFSLNGNFLGLLYNILHGKVVMPHRG 144

Query: 115 SPDYRSVVGFTDWR------KDLDSSIKPDDTFRYYGA-------LTMMAAKVSYEFLPF 174
           S  + S +G  D R      + L   I   D ++  G        L MMA+K++YE    
Sbjct: 145 SETFISAIGHLDGRINLYKSETLTKEIGEPDFWQKIGIGHRDLMDLCMMASKLAYENEKV 204

Query: 175 IQSVVNDHWKMKMLHYYAFWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQ 234
           +++VVN HWKM  + +Y  WNDF+ + +TQ F+  +   D+N+ +++FRGT P DA DW 
Sbjct: 205 VRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDANLILVSFRGTEPFDADDWI 264

Query: 235 VDADFSWYEIEGIGRIHSGFMKALGLQKATG--------WPKNL------------PPSP 294
            D D+SWYEI  +G++H GF++ALGL   T         +  NL            PPS 
Sbjct: 265 TDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFVNNLKFANLENVHATIPPSE 324

Query: 295 DNR-------------------------------------QFAYYTLRQQLRDIAKANDK 354
            ++                                     + AYY +R +L+ + K +  
Sbjct: 325 SSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPERTAYYVVRSKLKRLLKEHKN 384

Query: 355 ARFIITGHSLGGALATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHK 414
           A+F++TGHSLGGALA LF  +L  H+E  ++E+L  +YTYGQPRVG++Q   FM   +  
Sbjct: 385 AKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYGQPRVGNRQLGRFMEAHLEH 444

Query: 415 YGFKYYRYVYSNDLVPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLI 456
              KY+R VY NDLVPR+P D   F +KHFG C ++NSLY  + + E+PN NYF + +L+
Sbjct: 445 PVPKYFRVVYCNDLVPRLPYDNKTFLFKHFGICQYYNSLYIEQNINEEPNPNYFGMRFLV 504

BLAST of Lcy12g019760.1 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 6.5e-82
Identity = 169/491 (34.42%), Postives = 269/491 (54.79%), Query Frame = 0

Query: 11  SYMILKPENANLLDLFLF-ILPFGFRKRKFIDCPEGKEDS-----YRSFANRWLIFISML 70
           +Y+I++P     +DLF + +      K KF++ P+ +E S       +  +RW+I +S+L
Sbjct: 18  NYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDHRWVIVVSIL 77

Query: 71  LQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKL-------PDENSPDYRSVVGF 130
           ++  +  +  P+     ++    N  S NGG  GLL +L       P+  S  + S +G 
Sbjct: 78  VRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIPERGSVTFVSTIGQ 137

Query: 131 TDWRKDL-----------------DSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVN 190
            D R  L                    +K +   R    L +MA+K++YE    +++VV+
Sbjct: 138 LDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYENAKVVENVVD 197

Query: 191 DHWKMKMLHYYAFWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFS 250
            HWKM ++ +   WND+Q + +TQ F+F +   D+N+ VI+FRGT P DA DW  D D+S
Sbjct: 198 LHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDADDWGTDFDYS 257

Query: 251 WYEIEGIGRIHSGFMKALGL---QKATGWPKNLPPSPDNRQ-------------FAYYTL 310
           WYE+  +G++H GF++A+GL      T +  NL     + +              AYY +
Sbjct: 258 WYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLLDMVERSAYYAV 317

Query: 311 RQQLRDIAKANDKARFIITGHSLGGALATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGD 370
           R  L+ +   ++ ARF++TGHSLGGALA LF TLL  ++E+ ++++L  VYT+GQPR+G+
Sbjct: 318 RVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFGQPRIGN 377

Query: 371 QQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIPADGIVFEYKHFGGCVFFNSLYKGKILRE 430
           ++   FM   +++   +Y+R VY ND+VPR+P D   F YKHFG C+F++S Y      +
Sbjct: 378 REVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLFYDSFYNETKAED 437

Query: 431 QPNKNYFSLVWLIPKYLFAWWELIRSFIIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHF 456
           +P+ N + L + I  ++ A WEL+R   +    G  Y EG      R+ GL+IPG + H 
Sbjct: 438 EPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHGPDYKEGWFRILFRLMGLVIPGLSDHC 497

BLAST of Lcy12g019760.1 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 1.1e-76
Identity = 177/505 (35.05%), Postives = 262/505 (51.88%), Query Frame = 0

Query: 9   SKSYMILKPENANLLDLFLFILPFGFRK-RKFIDCPEGKEDSYRSFANRW--LIFISMLL 68
           S S++I+ P+    LDLF  I+        KF    + +  +  ++ +RW   +FI  +L
Sbjct: 9   STSHLIVSPDEGTFLDLFKHIVLSDLGSGAKFFRASDQRVPATAAYYSRWPVSVFICKIL 68

Query: 69  QLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLS-------KLPDENSPDYRSVVGFT 128
           QLF    AA L  L   LL   NF   N G LG+L        K+P     D+ S +G+ 
Sbjct: 69  QLF-QMPAAMLGHLTDFLL---NFYYQNHGFLGILRNIFLIRLKIPKRGEADFISTIGYL 128

Query: 129 DWRKDL------------------DSSIKPDDTFRYYGA--------LTMMAAKVSYEFL 188
           D R DL                  + S+K     +  GA        L +MA+K++YE  
Sbjct: 129 DSRMDLHGTPMVSHQADEVISNADNPSLKEGHNSKIKGALGNRSLMDLCIMASKLAYENT 188

Query: 189 PFIQSVVNDHWKMKMLHYYAFWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYD 248
             ++ VV +HWKM  +  Y   N FQ+   T AFIF +   D+N+ VI+FRGT P    +
Sbjct: 189 KVVERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANLIVISFRGTGPFSIPN 248

Query: 249 WQVDADFSWYEIEGIGRIHSGFMKALGL------------------QKATGWPKNLPPSP 308
           W  D DFS   +   G +H GF++A+GL                     T   K    +P
Sbjct: 249 WCTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINTKSPGSITELRKESEMAP 308

Query: 309 DNRQFA----YYTLRQQLRDIAKANDKARFIITGHSLGGALATLFVTLLSFHQESTLLEK 368
           D+  +A    Y+     L+ + K +  A+F++TGHSLGGALA LF  +L   QE+ +L++
Sbjct: 309 DHLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAILFTCILEIQQETEVLDR 368

Query: 369 LQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIPADGIVFEYKHFGGC 428
           L  VYT+GQPR+G+     FM N ++    +Y+R VY ND+VPR+P D + F ++HFG C
Sbjct: 369 LLNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDMVPRVPFDDVFFTFEHFGTC 428

Query: 429 VFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFIIPIVKGSVYYEGLLMKGV 456
           ++++S + G   +E+P++N F +   I  ++ AWWEL RSFI+  V G+ Y E    +  
Sbjct: 429 IYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFILNHVYGAEYKETWESRMF 488

BLAST of Lcy12g019760.1 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.3e-10
Identity = 58/197 (29.44%), Postives = 90/197 (45.69%), Query Frame = 0

Query: 173 FWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGI---GR 232
           F   F++        F  +   + + V++FRG+  ++  +W  + +F   EI  I    R
Sbjct: 73  FLYSFEDSGVGDVTGFLALDNTNKLIVLSFRGSRSIE--NWIGNLNFDLKEINDICSGCR 132

Query: 233 IHSGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGG 292
            H GF         + W           +    TLRQ++ D  + +   R + TGHSLGG
Sbjct: 133 GHDGF--------TSSW-----------RSVADTLRQKVEDAVREHPDYRVVFTGHSLGG 192

Query: 293 ALATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSN 352
           ALAT+    L  +           V++YG PRVG++ FAEF+     + G   YR  ++N
Sbjct: 193 ALATVAGADLRGNGYDI------DVFSYGAPRVGNRAFAEFLT---VQTGGTLYRITHTN 237

Query: 353 DLVPRIPADGIVFEYKH 367
           D+VPR+P     F Y H
Sbjct: 253 DIVPRLPPR--EFGYSH 237

BLAST of Lcy12g019760.1 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 1.1e-09
Identity = 58/179 (32.40%), Postives = 80/179 (44.69%), Query Frame = 0

Query: 194 DSNITV-IAFRGTSPLDAYDWQVDADF---SWYEIEGIGRIHSGFMKALGLQKATGWPKN 253
           DS  T+ I FRG+S +   +W  D  F   S+  + G  ++H GF+ + G          
Sbjct: 164 DSEKTIYIVFRGSSSI--RNWIADLTFVPVSYPPVSGT-KVHKGFLDSYG---------- 223

Query: 254 LPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLFVTLLSFHQESTLLE 313
                 N   A       + D  K     +  +TGHSLGGA A L   L  + +E  L  
Sbjct: 224 ---EVQNELVA------TVLDQFKQYPSYKVAVTGHSLGGATA-LLCALDLYQREEGLSS 283

Query: 314 KLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIPADGIVFEYKHFG 369
               +YT GQPRVGD  FA ++V+T    G  Y R V   D+VP +P     F + H G
Sbjct: 284 SNLFLYTQGQPRVGDPAFANYVVST----GIPYRRTVNERDIVPHLPP--AAFGFLHAG 313

BLAST of Lcy12g019760.1 vs. ExPASy TrEMBL
Match: A0A1S3BUY0 (uncharacterized protein LOC103493549 OS=Cucumis melo OX=3656 GN=LOC103493549 PE=4 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 2.2e-210
Identity = 348/483 (72.05%), Postives = 406/483 (84.06%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M+  E+EFSK+Y+ILKPENANLLDLFLF+LPFG RK+KFIDCP GKED + +FA+RWLIF
Sbjct: 1   MVLMENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFADRWLIF 60

Query: 61  ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKL--------PDENSPDYR 120
           +SMLLQ  L  IA PLA LD+ LLK FNFISFNGG+LG+LSK+          E+S +Y 
Sbjct: 61  VSMLLQFILALIATPLAKLDAFLLKLFNFISFNGGLLGILSKILRGKGFVKVKEDSSEYT 120

Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
           SVVGF DWR+DLDSSIK + +FRYY ALT+MA K+SYE  PF+QSVVND WKMK+L Y+ 
Sbjct: 121 SVVGFADWRRDLDSSIKSEKSFRYYSALTVMATKISYESKPFVQSVVNDRWKMKLLGYFN 180

Query: 181 FWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHS 240
           FWNDFQ K TTQAF F+N A D N+ ++AFRGTSPLDAYDWQVD D SWYEIEG+GRIHS
Sbjct: 181 FWNDFQGKATTQAFTFENTAIDPNVVIVAFRGTSPLDAYDWQVDVDLSWYEIEGVGRIHS 240

Query: 241 GFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALA 300
           GFMKALGLQKATGWPK LP +    +FAYYTLR+QLRDIAKAN+KARFI TGHSLGGALA
Sbjct: 241 GFMKALGLQKATGWPKELPKT-QTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALA 300

Query: 301 TLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLV 360
           TLFVT+LS H EST+LEKL +VYTYGQPRVGDQQFA+FMVN + KYGFKY+RYVYS DLV
Sbjct: 301 TLFVTVLSLHDESTILEKLDSVYTYGQPRVGDQQFAKFMVNCVQKYGFKYHRYVYSYDLV 360

Query: 361 PRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFI 420
           PR+P+D ++F+YKHFG CV+FNSLYKG+I++EQPN+NYFSL+W+IPKYL AWWELIRSFI
Sbjct: 361 PRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNRNYFSLLWVIPKYLSAWWELIRSFI 420

Query: 421 IPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNYV 476
           IP VKG  YYE LLMKG R+ GL+IPG +AHFP +YVN TRLG L +PD +EDP LG  +
Sbjct: 421 IPFVKGFDYYESLLMKGARLVGLLIPGLSAHFPLNYVNVTRLGKLNVPDEVEDPFLGYEI 480

BLAST of Lcy12g019760.1 vs. ExPASy TrEMBL
Match: A0A0A0L2D5 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639870 PE=4 SV=1)

HSP 1 Score: 735.3 bits (1897), Expect = 1.6e-208
Identity = 347/483 (71.84%), Postives = 404/483 (83.64%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M+  E+EFSK+Y+ILKPENANLLDLFLF+LPFG RK+KFIDCP GKED + +F +RWLI 
Sbjct: 1   MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV 60

Query: 61  ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKL--------PDENSPDYR 120
           +SMLLQ  L  IA PLA LD+ LLK FNFISFNGG LG+LSK+         +E+S +Y 
Sbjct: 61  VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT 120

Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
           SVVGF DWR+DLDSSIKP+ +FRYY ALT MA K+SYE  PF+QSVVND WKMK+L Y+ 
Sbjct: 121 SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN 180

Query: 181 FWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHS 240
           FWNDFQ +TTTQAF+F+N A D N+T++AFRGTSPLDAYDWQVD DFSWYEIE +G IHS
Sbjct: 181 FWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHS 240

Query: 241 GFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALA 300
           GFMKALGLQKATGWPK LP +    +FAYYTLR+QLRDIAKAN+KARFI TGHSLGGALA
Sbjct: 241 GFMKALGLQKATGWPKELPKT-QTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALA 300

Query: 301 TLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLV 360
           TLFVT+L  H EST+LEKL +VYTYGQPRVGDQQFA+FM+N I KYGFKY+RYVYS DLV
Sbjct: 301 TLFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLV 360

Query: 361 PRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFI 420
           PR+P+D ++F+YKHFG CV+FNSLYKG+I++EQPNKNYFSL+W+IPKYL AWWELIRSFI
Sbjct: 361 PRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFI 420

Query: 421 IPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNYV 476
           IP VKG  YYE LLMKG R+ GL+IPG  AHFP +YVN TRLG L +PD +EDPIL   +
Sbjct: 421 IPFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEI 480

BLAST of Lcy12g019760.1 vs. ExPASy TrEMBL
Match: A0A6J1FNK8 (uncharacterized protein LOC111445886 OS=Cucurbita moschata OX=3662 GN=LOC111445886 PE=4 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 1.2e-206
Identity = 350/484 (72.31%), Postives = 403/484 (83.26%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M+ KE EFSK+YMILKP +A LLDLFLF+LPFG RK KFIDCPEG EDSY +F +RW IF
Sbjct: 1   MVWKECEFSKNYMILKPNDAKLLDLFLFLLPFGLRKTKFIDCPEGNEDSYNNFTDRWSIF 60

Query: 61  ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKL--------PDENSPDYR 120
           IS+LLQ+ L A A PLA LDS L+  FNFISFNGGILGLL+K+        P+E+S DY 
Sbjct: 61  ISVLLQIILAAAATPLAKLDSFLVNLFNFISFNGGILGLLAKILKGKGLVKPNEDSSDYT 120

Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
           SVVGF DWR DLDSSI   DTFRYYG LT+MAAK++YE  PF+QSVVND WKMK+L ++ 
Sbjct: 121 SVVGFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFN 180

Query: 181 FWNDFQNKTTTQAFIFQNIATDS-NITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIH 240
           FWNDFQ K TTQAF+FQN ATD  NI VIAFRGTSPLD YDWQVDADFSWY+IEG+GRIH
Sbjct: 181 FWNDFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTYDWQVDADFSWYDIEGVGRIH 240

Query: 241 SGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGAL 300
           SGFMKALGLQKATGWPK L  +     FAYYTLRQ+LRDIAKAN KARFIITGHSLGGAL
Sbjct: 241 SGFMKALGLQKATGWPKEL--TKPQHDFAYYTLRQKLRDIAKANAKARFIITGHSLGGAL 300

Query: 301 ATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDL 360
           ATLFVTLLS H E+ +LEKLQ VYTYGQPRVGD++FAEFMVN++ +YGFKYYRYVYS+DL
Sbjct: 301 ATLFVTLLSLHDETPILEKLQGVYTYGQPRVGDRRFAEFMVNSVQQYGFKYYRYVYSSDL 360

Query: 361 VPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSF 420
           VPRIP+DG++F+YKHFG  ++FN+LYKG+I++ QPNKNYFSL+W+IPKYL AWWEL+RSF
Sbjct: 361 VPRIPSDGVIFKYKHFGRSIYFNTLYKGRIVKGQPNKNYFSLLWVIPKYLSAWWELVRSF 420

Query: 421 IIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNY 476
           I P+V G  YYE LLM   R+ GLIIPG AAHFP +YVNSTRLG L+ PD +EDPIL + 
Sbjct: 421 ITPLVWGFDYYESLLMSAARIIGLIIPGLAAHFPLNYVNSTRLGKLKAPDEVEDPILEDD 480

BLAST of Lcy12g019760.1 vs. ExPASy TrEMBL
Match: A0A6J1CEI9 (uncharacterized protein LOC111010491 OS=Momordica charantia OX=3673 GN=LOC111010491 PE=4 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 3.7e-205
Identity = 344/470 (73.19%), Postives = 401/470 (85.32%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKR-KFIDCPEGKEDSY-RSFANRWL 60
           M SKEHEFSK+Y+ILKP+NAN++DLFLF+LPFGFRKR KFIDCPEGKEDSY  SF NRWL
Sbjct: 1   MASKEHEFSKNYLILKPQNANIVDLFLFLLPFGFRKRTKFIDCPEGKEDSYTSSFTNRWL 60

Query: 61  IFISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKL--------PDENSPD 120
           IFIS++LQ+ L AIA PLA LD+ LL  FNFISFNGGILGLLS++        PDENS +
Sbjct: 61  IFISIVLQIILLAIATPLAKLDAFLLNVFNFISFNGGILGLLSRILKGESLVKPDENSAE 120

Query: 121 YRSVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHY 180
           Y SVVGF DWR+DLD SIK ++ FRYYG LT+MAA++SYE  PF+ SVVND WKMK+L +
Sbjct: 121 YTSVVGFADWRRDLDKSIKAEEIFRYYGGLTVMAAQLSYESKPFVLSVVNDRWKMKLLGH 180

Query: 181 YAFWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRI 240
           + FWNDFQ K TTQAF+FQN A D N+TV+AFRGTSPLDAYDWQVDADFSWY+IEG+GRI
Sbjct: 181 FNFWNDFQGKATTQAFMFQNTAADPNVTVVAFRGTSPLDAYDWQVDADFSWYDIEGVGRI 240

Query: 241 HSGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGA 300
           HSGFMKALGLQKATGWP+ L  S     FAYYTLRQ+LRDIAK+ND A+FIITGHSLGGA
Sbjct: 241 HSGFMKALGLQKATGWPEELVKS--KHHFAYYTLRQKLRDIAKSNDNAKFIITGHSLGGA 300

Query: 301 LATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSND 360
           LA LFVT+LS H++S +LEKLQAVYTY QPRVGD++FAEFMVNT+ KYGFKY+RYVYS+D
Sbjct: 301 LAALFVTVLSLHRDSAVLEKLQAVYTYSQPRVGDRRFAEFMVNTVKKYGFKYHRYVYSSD 360

Query: 361 LVPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRS 420
           LVPR+P DG++F+YKHFG C++FNSLYKG+I++E+PNKNYFSL+W++PKYL AWWELIRS
Sbjct: 361 LVPRVPFDGVIFKYKHFGRCIYFNSLYKGRIVKEEPNKNYFSLIWVMPKYLNAWWELIRS 420

Query: 421 FIIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIP 461
           FI+P+VKG  Y+E LLMKG RV GL IPG AAH P DYVNS RLG    P
Sbjct: 421 FIVPLVKGFDYHESLLMKGARVVGLFIPGLAAHIPTDYVNSIRLGKFNPP 468

BLAST of Lcy12g019760.1 vs. ExPASy TrEMBL
Match: A0A6J1J3Y9 (uncharacterized protein LOC111481121 OS=Cucurbita maxima OX=3661 GN=LOC111481121 PE=4 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 1.8e-204
Identity = 347/484 (71.69%), Postives = 404/484 (83.47%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M+SK+ EFSK+YMILKP +A LLDLFLF+LPFG RK KFIDCPEG E SY +F +RW IF
Sbjct: 1   MVSKKCEFSKNYMILKPNDAKLLDLFLFLLPFGLRKTKFIDCPEGNEASYSNFTDRWFIF 60

Query: 61  ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKL--------PDENSPDYR 120
           IS+LLQ+ L A A PL+ LDS L+  FNFISFNGGILGLLSK+        P+E+S DY 
Sbjct: 61  ISILLQIILAAAATPLSKLDSFLVNLFNFISFNGGILGLLSKILKGKGLVKPNEDSSDYT 120

Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
           SV+GF DWR DLDSSI   DTFRYYG LT+MAAK++YE  PF+QSVVND WKMK+L ++ 
Sbjct: 121 SVLGFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFN 180

Query: 181 FWNDFQNKTTTQAFIFQNIATDS-NITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIH 240
           FWNDFQ K TTQAF+FQN ATD  NI VIAFRGTSPLD  DWQV+ADFSWY+IEG+GRIH
Sbjct: 181 FWNDFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTDDWQVNADFSWYDIEGLGRIH 240

Query: 241 SGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGAL 300
           SGFMKALGLQKATGWPK L  +     FAYY+LRQ+LRDIAKAN KARFIITGHSLGGAL
Sbjct: 241 SGFMKALGLQKATGWPKEL--TKPQHDFAYYSLRQKLRDIAKANAKARFIITGHSLGGAL 300

Query: 301 ATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDL 360
           ATLFVTLLS H E+T+LEKLQ VYTYGQPRVGD++FAEFMVNT+ KYGFKYYRYVYS+DL
Sbjct: 301 ATLFVTLLSLHDETTILEKLQGVYTYGQPRVGDRRFAEFMVNTVQKYGFKYYRYVYSSDL 360

Query: 361 VPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSF 420
           VPRIP+DG++F+YKHFG  ++FNSLYKG+I++EQPNKNYFSL+W+IPKYL AWWEL+RSF
Sbjct: 361 VPRIPSDGVIFKYKHFGRSIYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELVRSF 420

Query: 421 IIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNY 476
           I P++ G  +YE LLM   R+ GLIIPG AAHFP +YVNS RLG L++PD IEDPIL + 
Sbjct: 421 ITPLMWGFDHYESLLMSAARMVGLIIPGLAAHFPLNYVNSIRLGKLKVPDEIEDPILEDD 480

BLAST of Lcy12g019760.1 vs. NCBI nr
Match: XP_038899591.1 (triacylglycerol lipase OBL1-like [Benincasa hispida])

HSP 1 Score: 760.0 bits (1961), Expect = 1.3e-215
Identity = 361/479 (75.37%), Postives = 413/479 (86.22%), Query Frame = 0

Query: 5   EHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIFISML 64
           E EFSK+Y+ILKPENANLLDLFLF+LPFG RKRKFIDCP GKED +++F +RWLIFISML
Sbjct: 5   ESEFSKNYVILKPENANLLDLFLFLLPFGLRKRKFIDCPLGKEDPFKNFRDRWLIFISML 64

Query: 65  LQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKL--------PDENSPDYRSVVG 124
           LQ  L  IA PLA LDS LLK  NFISFNGG+ G+LSK+         +E+SP+Y SVVG
Sbjct: 65  LQFILAIIATPLAKLDSFLLKLLNFISFNGGVSGILSKILKGKSFLEVNEDSPEYTSVVG 124

Query: 125 FTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYAFWND 184
           F DWR+DLDSSIKPDDTFRYY ALT+MAAKVSYE  PF+QSVVND WKMK+L Y+ FWND
Sbjct: 125 FADWRRDLDSSIKPDDTFRYYSALTVMAAKVSYESKPFVQSVVNDRWKMKLLGYFNFWND 184

Query: 185 FQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMK 244
           FQ K TTQAF+F+N A D N+ V+AFRGTS LD+YDWQVDADFSWYEIEG+GRIHSGFMK
Sbjct: 185 FQGKATTQAFMFENTAIDPNVIVVAFRGTSLLDSYDWQVDADFSWYEIEGVGRIHSGFMK 244

Query: 245 ALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLFV 304
           ALGLQKATGWPK LP S   ++FAYYT+RQ+LRDIAK NDKARFIITGHSLGGALATLFV
Sbjct: 245 ALGLQKATGWPKELPKS-QLQEFAYYTIRQKLRDIAKTNDKARFIITGHSLGGALATLFV 304

Query: 305 TLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIP 364
           T+LS H EST+LEKL+ +YTYGQPRVGDQQFA+FMVNT+ KYGFKY+RYVYS+DLVPR+P
Sbjct: 305 TVLSLHDESTILEKLEGIYTYGQPRVGDQQFAKFMVNTVQKYGFKYHRYVYSSDLVPRLP 364

Query: 365 ADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFIIPIV 424
           +D I+F+YKHFG CVFFN+LY+G+I++EQPNKNYFSLVW+IPKYL AWWELIRS IIP+V
Sbjct: 365 SDAILFKYKHFGRCVFFNTLYRGRIVKEQPNKNYFSLVWVIPKYLSAWWELIRSLIIPLV 424

Query: 425 KGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNYVEAD 476
           KG  YYE LLMKG RV GL IPG AAHFP +YVNSTRLG L +PD +EDPILG+ +E D
Sbjct: 425 KGFDYYESLLMKGGRVVGLFIPGLAAHFPLNYVNSTRLGKLNVPDKVEDPILGDDIEDD 482

BLAST of Lcy12g019760.1 vs. NCBI nr
Match: XP_008452563.1 (PREDICTED: uncharacterized protein LOC103493549 [Cucumis melo])

HSP 1 Score: 741.5 bits (1913), Expect = 4.6e-210
Identity = 348/483 (72.05%), Postives = 406/483 (84.06%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M+  E+EFSK+Y+ILKPENANLLDLFLF+LPFG RK+KFIDCP GKED + +FA+RWLIF
Sbjct: 1   MVLMENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFADRWLIF 60

Query: 61  ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKL--------PDENSPDYR 120
           +SMLLQ  L  IA PLA LD+ LLK FNFISFNGG+LG+LSK+          E+S +Y 
Sbjct: 61  VSMLLQFILALIATPLAKLDAFLLKLFNFISFNGGLLGILSKILRGKGFVKVKEDSSEYT 120

Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
           SVVGF DWR+DLDSSIK + +FRYY ALT+MA K+SYE  PF+QSVVND WKMK+L Y+ 
Sbjct: 121 SVVGFADWRRDLDSSIKSEKSFRYYSALTVMATKISYESKPFVQSVVNDRWKMKLLGYFN 180

Query: 181 FWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHS 240
           FWNDFQ K TTQAF F+N A D N+ ++AFRGTSPLDAYDWQVD D SWYEIEG+GRIHS
Sbjct: 181 FWNDFQGKATTQAFTFENTAIDPNVVIVAFRGTSPLDAYDWQVDVDLSWYEIEGVGRIHS 240

Query: 241 GFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALA 300
           GFMKALGLQKATGWPK LP +    +FAYYTLR+QLRDIAKAN+KARFI TGHSLGGALA
Sbjct: 241 GFMKALGLQKATGWPKELPKT-QTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALA 300

Query: 301 TLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLV 360
           TLFVT+LS H EST+LEKL +VYTYGQPRVGDQQFA+FMVN + KYGFKY+RYVYS DLV
Sbjct: 301 TLFVTVLSLHDESTILEKLDSVYTYGQPRVGDQQFAKFMVNCVQKYGFKYHRYVYSYDLV 360

Query: 361 PRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFI 420
           PR+P+D ++F+YKHFG CV+FNSLYKG+I++EQPN+NYFSL+W+IPKYL AWWELIRSFI
Sbjct: 361 PRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNRNYFSLLWVIPKYLSAWWELIRSFI 420

Query: 421 IPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNYV 476
           IP VKG  YYE LLMKG R+ GL+IPG +AHFP +YVN TRLG L +PD +EDP LG  +
Sbjct: 421 IPFVKGFDYYESLLMKGARLVGLLIPGLSAHFPLNYVNVTRLGKLNVPDEVEDPFLGYEI 480

BLAST of Lcy12g019760.1 vs. NCBI nr
Match: XP_011654113.1 (uncharacterized protein LOC101203640 [Cucumis sativus] >KGN55199.1 hypothetical protein Csa_012910 [Cucumis sativus])

HSP 1 Score: 735.3 bits (1897), Expect = 3.3e-208
Identity = 347/483 (71.84%), Postives = 404/483 (83.64%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M+  E+EFSK+Y+ILKPENANLLDLFLF+LPFG RK+KFIDCP GKED + +F +RWLI 
Sbjct: 1   MVLIENEFSKNYVILKPENANLLDLFLFLLPFGLRKKKFIDCPYGKEDYFTNFTDRWLIV 60

Query: 61  ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKL--------PDENSPDYR 120
           +SMLLQ  L  IA PLA LD+ LLK FNFISFNGG LG+LSK+         +E+S +Y 
Sbjct: 61  VSMLLQFILSLIATPLAKLDAFLLKLFNFISFNGGFLGILSKILRGKGFVKVNEDSAEYT 120

Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
           SVVGF DWR+DLDSSIKP+ +FRYY ALT MA K+SYE  PF+QSVVND WKMK+L Y+ 
Sbjct: 121 SVVGFADWRRDLDSSIKPEQSFRYYSALTAMATKISYESKPFVQSVVNDRWKMKLLGYFN 180

Query: 181 FWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHS 240
           FWNDFQ +TTTQAF+F+N A D N+T++AFRGTSPLDAYDWQVD DFSWYEIE +G IHS
Sbjct: 181 FWNDFQGRTTTQAFMFKNTAIDPNVTIVAFRGTSPLDAYDWQVDFDFSWYEIEDVGLIHS 240

Query: 241 GFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALA 300
           GFMKALGLQKATGWPK LP +    +FAYYTLR+QLRDIAKAN+KARFI TGHSLGGALA
Sbjct: 241 GFMKALGLQKATGWPKELPKT-QTHEFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALA 300

Query: 301 TLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLV 360
           TLFVT+L  H EST+LEKL +VYTYGQPRVGDQQFA+FM+N I KYGFKY+RYVYS DLV
Sbjct: 301 TLFVTVLCLHDESTILEKLDSVYTYGQPRVGDQQFAKFMLNCIQKYGFKYHRYVYSYDLV 360

Query: 361 PRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFI 420
           PR+P+D ++F+YKHFG CV+FNSLYKG+I++EQPNKNYFSL+W+IPKYL AWWELIRSFI
Sbjct: 361 PRVPSDAVLFKYKHFGRCVYFNSLYKGRIVKEQPNKNYFSLLWVIPKYLSAWWELIRSFI 420

Query: 421 IPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNYV 476
           IP VKG  YYE LLMKG R+ GL+IPG  AHFP +YVN TRLG L +PD +EDPIL   +
Sbjct: 421 IPFVKGFDYYESLLMKGARLVGLVIPGLTAHFPLNYVNVTRLGKLNVPDEVEDPILRYEI 480

BLAST of Lcy12g019760.1 vs. NCBI nr
Match: XP_022940183.1 (uncharacterized protein LOC111445886 [Cucurbita moschata])

HSP 1 Score: 729.2 bits (1881), Expect = 2.4e-206
Identity = 350/484 (72.31%), Postives = 403/484 (83.26%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M+ KE EFSK+YMILKP +A LLDLFLF+LPFG RK KFIDCPEG EDSY +F +RW IF
Sbjct: 1   MVWKECEFSKNYMILKPNDAKLLDLFLFLLPFGLRKTKFIDCPEGNEDSYNNFTDRWSIF 60

Query: 61  ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKL--------PDENSPDYR 120
           IS+LLQ+ L A A PLA LDS L+  FNFISFNGGILGLL+K+        P+E+S DY 
Sbjct: 61  ISVLLQIILAAAATPLAKLDSFLVNLFNFISFNGGILGLLAKILKGKGLVKPNEDSSDYT 120

Query: 121 SVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYA 180
           SVVGF DWR DLDSSI   DTFRYYG LT+MAAK++YE  PF+QSVVND WKMK+L ++ 
Sbjct: 121 SVVGFADWRTDLDSSIPLHDTFRYYGGLTIMAAKIAYESKPFVQSVVNDRWKMKLLGFFN 180

Query: 181 FWNDFQNKTTTQAFIFQNIATDS-NITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIH 240
           FWNDFQ K TTQAF+FQN ATD  NI VIAFRGTSPLD YDWQVDADFSWY+IEG+GRIH
Sbjct: 181 FWNDFQGKATTQAFMFQNTATDDPNIIVIAFRGTSPLDTYDWQVDADFSWYDIEGVGRIH 240

Query: 241 SGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGAL 300
           SGFMKALGLQKATGWPK L  +     FAYYTLRQ+LRDIAKAN KARFIITGHSLGGAL
Sbjct: 241 SGFMKALGLQKATGWPKEL--TKPQHDFAYYTLRQKLRDIAKANAKARFIITGHSLGGAL 300

Query: 301 ATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDL 360
           ATLFVTLLS H E+ +LEKLQ VYTYGQPRVGD++FAEFMVN++ +YGFKYYRYVYS+DL
Sbjct: 301 ATLFVTLLSLHDETPILEKLQGVYTYGQPRVGDRRFAEFMVNSVQQYGFKYYRYVYSSDL 360

Query: 361 VPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSF 420
           VPRIP+DG++F+YKHFG  ++FN+LYKG+I++ QPNKNYFSL+W+IPKYL AWWEL+RSF
Sbjct: 361 VPRIPSDGVIFKYKHFGRSIYFNTLYKGRIVKGQPNKNYFSLLWVIPKYLSAWWELVRSF 420

Query: 421 IIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDPILGNY 476
           I P+V G  YYE LLM   R+ GLIIPG AAHFP +YVNSTRLG L+ PD +EDPIL + 
Sbjct: 421 ITPLVWGFDYYESLLMSAARIIGLIIPGLAAHFPLNYVNSTRLGKLKAPDEVEDPILEDD 480

BLAST of Lcy12g019760.1 vs. NCBI nr
Match: XP_022139642.1 (uncharacterized protein LOC111010491 [Momordica charantia])

HSP 1 Score: 724.2 bits (1868), Expect = 7.7e-205
Identity = 344/470 (73.19%), Postives = 401/470 (85.32%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKR-KFIDCPEGKEDSY-RSFANRWL 60
           M SKEHEFSK+Y+ILKP+NAN++DLFLF+LPFGFRKR KFIDCPEGKEDSY  SF NRWL
Sbjct: 1   MASKEHEFSKNYLILKPQNANIVDLFLFLLPFGFRKRTKFIDCPEGKEDSYTSSFTNRWL 60

Query: 61  IFISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKL--------PDENSPD 120
           IFIS++LQ+ L AIA PLA LD+ LL  FNFISFNGGILGLLS++        PDENS +
Sbjct: 61  IFISIVLQIILLAIATPLAKLDAFLLNVFNFISFNGGILGLLSRILKGESLVKPDENSAE 120

Query: 121 YRSVVGFTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHY 180
           Y SVVGF DWR+DLD SIK ++ FRYYG LT+MAA++SYE  PF+ SVVND WKMK+L +
Sbjct: 121 YTSVVGFADWRRDLDKSIKAEEIFRYYGGLTVMAAQLSYESKPFVLSVVNDRWKMKLLGH 180

Query: 181 YAFWNDFQNKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRI 240
           + FWNDFQ K TTQAF+FQN A D N+TV+AFRGTSPLDAYDWQVDADFSWY+IEG+GRI
Sbjct: 181 FNFWNDFQGKATTQAFMFQNTAADPNVTVVAFRGTSPLDAYDWQVDADFSWYDIEGVGRI 240

Query: 241 HSGFMKALGLQKATGWPKNLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGA 300
           HSGFMKALGLQKATGWP+ L  S     FAYYTLRQ+LRDIAK+ND A+FIITGHSLGGA
Sbjct: 241 HSGFMKALGLQKATGWPEELVKS--KHHFAYYTLRQKLRDIAKSNDNAKFIITGHSLGGA 300

Query: 301 LATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSND 360
           LA LFVT+LS H++S +LEKLQAVYTY QPRVGD++FAEFMVNT+ KYGFKY+RYVYS+D
Sbjct: 301 LAALFVTVLSLHRDSAVLEKLQAVYTYSQPRVGDRRFAEFMVNTVKKYGFKYHRYVYSSD 360

Query: 361 LVPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRS 420
           LVPR+P DG++F+YKHFG C++FNSLYKG+I++E+PNKNYFSL+W++PKYL AWWELIRS
Sbjct: 361 LVPRVPFDGVIFKYKHFGRCIYFNSLYKGRIVKEEPNKNYFSLIWVMPKYLNAWWELIRS 420

Query: 421 FIIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIP 461
           FI+P+VKG  Y+E LLMKG RV GL IPG AAH P DYVNS RLG    P
Sbjct: 421 FIVPLVKGFDYHESLLMKGARVVGLFIPGLAAHIPTDYVNSIRLGKFNPP 468

BLAST of Lcy12g019760.1 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 425.6 bits (1093), Expect = 5.2e-119
Identity = 215/470 (45.74%), Postives = 297/470 (63.19%), Query Frame = 0

Query: 7   EFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSY-RSFANRWLIFISMLL 66
           +F  SY ++ P  A+ LDL L +        +FID P      + RSFA+RW++ +++ L
Sbjct: 7   KFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAIFL 66

Query: 67  QLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKL-------PDENSPDYRSVVGFT 126
           Q  L  ++ P A +   L    N ++ NGG   L+  L       PD++S  Y S +G +
Sbjct: 67  QKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGKLVKPDKSSATYTSFIGCS 126

Query: 127 DWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYAFWNDFQ 186
           D R +LD  I    +  Y   L++MA+K+SYE  P+I SVV + WKM ++  Y F+N FQ
Sbjct: 127 DRRIELDEKINV-GSIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNAFQ 186

Query: 187 NKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMKAL 246
               TQAF+F+  +T+ ++ V++FRGT P +A DW  D D SWYE++ +G++H+GF +AL
Sbjct: 187 ESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRAL 246

Query: 247 GLQKATGWPK-NLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLFVT 306
           GLQK  GWPK N+       Q+AYYT+RQ LRD    N   ++I+TGHSLGGALA LF  
Sbjct: 247 GLQK-DGWPKENISLL---HQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPA 306

Query: 307 LLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIPA 366
           +L+ H E  LL+KL+ +YT+GQPRVGD+ F EFM   + K+G +Y R+VY+ND+VPR+P 
Sbjct: 307 ILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPF 366

Query: 367 DG-IVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFIIPIV 426
           D   +F YKH+G C  FNSLYKGK+  + PN NYF+L+WLIP+ L   WE IRSFI+   
Sbjct: 367 DDKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQLLTGLWEFIRSFILQFW 426

Query: 427 KGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIEDP 467
           KG  Y E  LM+ VRV G++ PG + HFP DYVNSTRLG L  P     P
Sbjct: 427 KGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLVRPPPTTTP 471

BLAST of Lcy12g019760.1 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 419.5 bits (1077), Expect = 3.8e-117
Identity = 205/464 (44.18%), Postives = 299/464 (64.44%), Query Frame = 0

Query: 9   SKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGK-EDSYRSFANRWLIFISMLLQL 68
           +K+Y +L P  A + DL   +       RKFI   E + ED    F  RW+IF+S+++Q 
Sbjct: 4   TKNYFVLDPREATVSDLIHLLFSSDLGDRKFIHSSEERIEDDLSRFRRRWIIFVSIVIQK 63

Query: 69  FLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKL-------PDENSPDYRSVVGFTDW 128
            +     PL  +   L    N +S NGG L +L  L       P++ S  + S+ G  D 
Sbjct: 64  LIILFKKPLYFVGFSLACWLNLLSSNGGFLMILPNLFKGNIIWPEKASATFASLNGNLDR 123

Query: 129 RKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYAFWNDFQNK 188
           R +L+  ++     RY   L++MA+K+SYE + F+ SV+++HWKM +L +Y+ WN +Q +
Sbjct: 124 RVELNPKVERGSK-RYKAMLSIMASKLSYENINFVSSVLHNHWKMDLLGFYSCWNGYQKQ 183

Query: 189 TTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMKALGL 248
            +T+  + ++ +TD N+ +++FRGT P DA DW  D D SWYE++ +G+IH GFMKALGL
Sbjct: 184 RSTEVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEVKNVGKIHGGFMKALGL 243

Query: 249 QKATGWPK--NLPPSPD-NRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLFVT 308
           QK  GWPK  NL  + +    +AYYT+R+ L++I   N  ++FI+TGHSLGGALA LF  
Sbjct: 244 QK-EGWPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKFILTGHSLGGALAILFTA 303

Query: 309 LLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIPA 368
           +L  H E  +LE+L+ VYT+GQPRVGD++F  FM +++ K+  KY RYVY ND+VPR+P 
Sbjct: 304 VLVMHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKDSLKKFDVKYERYVYCNDMVPRLPF 363

Query: 369 DGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFIIPIVK 428
           D     +KHFG C++++S YKGK+  E+PNKNYF+LVW++PK + A WELIRSF++P  K
Sbjct: 364 DDKTLMFKHFGACLYYDSFYKGKVEEEEPNKNYFNLVWVLPKIMNALWELIRSFVMPYWK 423

Query: 429 GSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPD 462
           G  + EG  ++  RV  L+IPG  AHFP +Y+N T LG   +PD
Sbjct: 424 GGEFREGWFLRCFRVVALLIPGLPAHFPNEYINVTLLG--DLPD 463

BLAST of Lcy12g019760.1 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 391.3 bits (1004), Expect = 1.1e-108
Identity = 204/476 (42.86%), Postives = 284/476 (59.66%), Query Frame = 0

Query: 1   MISKEHEFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSYRSFANRWLIF 60
           M S + +  + Y+IL+PE     +L   +      K + +D  E +E S+R   +RWLIF
Sbjct: 2   MNSDDDDEPRGYLILRPEELRPWELVRLLFSGDIEKPRSVDSSETEEHSFR---HRWLIF 61

Query: 61  ISMLLQLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSK----LPDENSPDYRSVVG 120
           +S++L   L   +  LA + S L    NF+S N    GL  +    +P   S +Y+S +G
Sbjct: 62  VSLVLLKLLRFFSKLLALVGSALEFSLNFLS-NNSFSGLFLRGEVVMPQRTSENYQSFIG 121

Query: 121 FTDWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYAFWND 180
             D R  LD ++  +D  +YY AL++MA+K++YE    I+ VV +HW MK L    +WN+
Sbjct: 122 HLDTRVSLDMTLNREDGEKYYAALSIMASKIAYENAARIKHVVENHWNMKYLGLVDYWNE 181

Query: 181 FQNKTTTQAFIFQNIAT------DSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRI 240
           +Q K TTQAFI     T           V+AFRGT   ++ DW  D D +W+E+  IG I
Sbjct: 182 YQEKETTQAFIMSTDETTTRSNGQETTVVVAFRGTELFNSEDWCSDFDITWFELPNIGNI 241

Query: 241 HSGFMKALGLQKATGWPKNLPPSPDNRQ-FAYYTLRQQLRDIAKANDKARFIITGHSLGG 300
           H GFMKALGLQ    WPK    +PD +   AYY++R  L+ +   N   +F++TGHSLGG
Sbjct: 242 HGGFMKALGLQNNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQNKNTKFVLTGHSLGG 301

Query: 301 ALATLFVTLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSN 360
           ALA LF  +L  H E+ LLE++Q VYTYGQPRVGD +F EFM   + KY  KYYR+VY+N
Sbjct: 302 ALAILFTAVLVIHHETELLERIQGVYTYGQPRVGDSKFGEFMEKKLEKYNIKYYRFVYNN 361

Query: 361 DLVPRIPADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIR 420
           D+VPR+P D     +KHFG C++++  Y+ K++REQ ++N+F L  +I     A  E IR
Sbjct: 362 DIVPRLPYDDKDLMFKHFGTCIYYDQNYQAKVMREQSDENFFLLRGIIKMMWSAILEFIR 421

Query: 421 SFIIPIVKGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLLQIPDVIED 466
           SF I   KGS Y EG L+KG R  G+I+PG + H PQDYVN+TRL L  +  V  D
Sbjct: 422 SFTIVAEKGSEYSEGWLLKGGRALGIIVPGVSNHTPQDYVNATRLTLPCVFQVYRD 473

BLAST of Lcy12g019760.1 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 379.4 bits (973), Expect = 4.3e-105
Identity = 197/467 (42.18%), Postives = 283/467 (60.60%), Query Frame = 0

Query: 10  KSYMILKPENANLLDLFLFILPFGFRKRKFIDCP-EGKEDSYRSFANRWLIFISMLLQLF 69
           K+Y +L P  A + DL   +       RKF+D   E  E+    F  RW+IF+S+++Q  
Sbjct: 5   KNYFVLDPREATVTDLMRLLFYSDLENRKFVDTSVENLENRLCEFRGRWIIFVSIVVQKL 64

Query: 70  LGAIAAPLAALDSILLKHFNFISFNGGILGLLSKL-------PDENSPDYRSVVGFTDWR 129
           L  +  PL+ L   L    N  S NGG   +   L       P++ S  + S+ G  D +
Sbjct: 65  LIILRKPLSFLGFALGFWLNLPSSNGGFFKIFLNLVKGRFIWPEKTSATFASINGNLDQK 124

Query: 130 KD--LDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYAFWNDFQN 189
            +  L  SIK  D  RY   L++MA+K++YE   FI+SV+ DHW+M +L +Y+  NDF  
Sbjct: 125 VELGLGRSIKIGDE-RYKPLLSIMASKLAYENEHFIRSVLQDHWQMDLLGFYSCPNDFDQ 184

Query: 190 KTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMKALG 249
             +T+  + ++   + N+ V++FRGT P +A DW  D D SW+ +  +G+IH GFMKALG
Sbjct: 185 TRSTEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNVMNVGKIHGGFMKALG 244

Query: 250 LQKATGWPKNLPPSPDNR---QFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLFV 309
           L K  GW + +          Q AYYT+ +QL+++ + N  ++FI++GHSLGGALA LF 
Sbjct: 245 LPK-EGWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKFILSGHSLGGALAILFT 304

Query: 310 TLLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIP 369
            +L  H E  +LE+L+ VYT+GQPRVGD+ F  +M + + ++  KY RYVY ND+VPR+P
Sbjct: 305 AVLIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKDKLKEFDVKYKRYVYCNDMVPRLP 364

Query: 370 ADGIVFEYKHFGGCVFFNSLYKGKILREQPNKNYFSLVWLIPKYLFAWWELIRSFIIPIV 429
            D     +KHFGGC++ +S YKGK+  E+PNKNYF++ W+IPK + A WELIRSFII   
Sbjct: 365 FDDKTLMFKHFGGCLYCDSFYKGKVEEEEPNKNYFNIFWVIPKIINAVWELIRSFIISCW 424

Query: 430 KGSVYYEGLLMKGVRVFGLIIPGAAAHFPQDYVNSTRLGLL--QIPD 462
           +G  Y EG L+   R+  L+IPG  AHFP +YVN   LG    Q+PD
Sbjct: 425 QGREYREGWLLTCFRLVALLIPGLPAHFPNEYVNVALLGNFPPQVPD 469

BLAST of Lcy12g019760.1 vs. TAIR 10
Match: AT1G45201.2 (triacylglycerol lipase-like 1 )

HSP 1 Score: 336.3 bits (861), Expect = 4.2e-92
Identity = 170/385 (44.16%), Postives = 243/385 (63.12%), Query Frame = 0

Query: 7   EFSKSYMILKPENANLLDLFLFILPFGFRKRKFIDCPEGKEDSY-RSFANRWLIFISMLL 66
           +F  SY ++ P  A+ LDL L +        +FID P      + RSFA+RW++ +++ L
Sbjct: 7   KFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAIFL 66

Query: 67  QLFLGAIAAPLAALDSILLKHFNFISFNGGILGLLSKL-------PDENSPDYRSVVGFT 126
           Q  L  ++ P A +   L    N ++ NGG   L+  L       PD++S  Y S +G +
Sbjct: 67  QKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGKLVKPDKSSATYTSFIGCS 126

Query: 127 DWRKDLDSSIKPDDTFRYYGALTMMAAKVSYEFLPFIQSVVNDHWKMKMLHYYAFWNDFQ 186
           D R +LD  I    +  Y   L++MA+K+SYE  P+I SVV + WKM ++  Y F+N FQ
Sbjct: 127 DRRIELDEKINV-GSIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNAFQ 186

Query: 187 NKTTTQAFIFQNIATDSNITVIAFRGTSPLDAYDWQVDADFSWYEIEGIGRIHSGFMKAL 246
               TQAF+F+  +T+ ++ V++FRGT P +A DW  D D SWYE++ +G++H+GF +AL
Sbjct: 187 ESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRAL 246

Query: 247 GLQKATGWPK-NLPPSPDNRQFAYYTLRQQLRDIAKANDKARFIITGHSLGGALATLFVT 306
           GLQK  GWPK N+       Q+AYYT+RQ LRD    N   ++I+TGHSLGGALA LF  
Sbjct: 247 GLQK-DGWPKENISLL---HQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPA 306

Query: 307 LLSFHQESTLLEKLQAVYTYGQPRVGDQQFAEFMVNTIHKYGFKYYRYVYSNDLVPRIPA 366
           +L+ H E  LL+KL+ +YT+GQPRVGD+ F EFM   + K+G +Y R+VY+ND+VPR+P 
Sbjct: 307 ILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPF 366

Query: 367 DG-IVFEYKHFGGCVFFNSLYKGKI 382
           D   +F YKH+G C  FNSLYKGK+
Sbjct: 367 DDKYLFSYKHYGPCNSFNSLYKGKV 386