Lcy12g017850.1 (mRNA) Sponge gourd (P93075) v1

Overview


Name	Lcy12g017850.1
Type	mRNA
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	RNase H domain-containing protein
Location	Chr12: 45375161 .. 45376065 (-)
Sequence length	661
RNA-Seq Expression	Lcy12g017850.1
Synteny	Lcy12g017850.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTAGATTTATAAAGTTAGATATTTTATTTGGAGAACTCTCCATCATTGTATTCCCTGTATGGTGAATCTCAGAAAGCATCATGTTCCAGTAGAGGTAACGTGCCCATTATGCTTGGATGACTTCGAAGATACGGTCCATGCTTTGTTCTTGTATCGCCGAGCTCATGAAGTTTGGTCTATGCTGGGTTTATGGGAGGAGATTGGCCCAGATCTGATGAGGGAGGTTCAAGATCGATGGATATATATATGTAATAGAGCTTCCACAAGTATCCTTGAAAGGATATGTGTGAGTGCTTGGGCCATATGGAAAGATCGTAATAATATCGTACACCAACGTTCAGTTCCATCAGTGGACTTATTATGTGGTTGGATCCAGGAGTATATCTCGGAGTTCCAGGGGATTAATCAAAAGTCGGGTCGTCGAGTTCAACCGATGGATAAGGTGTCTGATATGATTTCAAGTGCAGGCAATTATCTTGCATGTTGATGCGGCATGTGATGATGTTGGTAGGCGTAGTGGGGTGGGTGCCGTTATTCGAGATAAGGAGGGACAGTTGGTGGCTTCTCTTTCAATGGGAACGTCTATTTACTCGGCTCCTTTATGCGCCGAGGCTGTAGCTGTTCTTGAGGGTCTCAAGTTGACAATTCAGCAGAATATTCGTTGTGTGACAGTTTGTTCGGATTCCCTTGCGTTGATCCAAATTCTGCTCCATAGAAGTCGATGTCCTGTAGACTGTGCCAACATTGTTGCGAACATTCATCTTGTAGGGGGATCGTTCCAAACCATCAATTTTTGTCATGTAAATCGTGAGTATAATTTGTTAGCTCATGAATTAGCTCTATTAGGATTATGTTCACCTCTTGTGTTTGGTATAGGGATTTCCCATAGTGGGTTGTTATTCTGA

mRNA sequence

TTAGATTTATAAAGTTAGATATTTTATTTGGAGAACTCTCCATCATTGTATTCCCTGTATGGTGAATCTCAGAAAGCATCATGTTCCAGTAGAGGTAACGTGCCCATTATGCTTGGATGACTTCGAAGATACGGTCCATGCTTTGTTCTTGTATCGCCGAGCTCATGAAGTTTGGTCTATGCTGGGTTTATGGGAGGAGATTGGCCCAGATCTGATGAGGGAGGCAATTATCTTGCATGTTGATGCGGCATGTGATGATGTTGGTAGGCGTAGTGGGGTGGGTGCCGTTATTCGAGATAAGGAGGGACAGTTGGTGGCTTCTCTTTCAATGGGAACGTCTATTTACTCGGCTCCTTTATGCGCCGAGGCTGTAGCTGTTCTTGAGGGTCTCAAGTTGACAATTCAGCAGAATATTCGTTGTGTGACAGTTTGTTCGGATTCCCTTGCGTTGATCCAAATTCTGCTCCATAGAAGTCGATGTCCTGTAGACTGTGCCAACATTGTTGCGAACATTCATCTTGTAGGGGGATCGTTCCAAACCATCAATTTTTGTCATGTAAATCGTGAGTATAATTTGTTAGCTCATGAATTAGCTCTATTAGGATTATGTTCACCTCTTGTGTTTGGTATAGGGATTTCCCATAGTGGGTTGTTATTCTGA

Coding sequence (CDS)

ATGGTGAATCTCAGAAAGCATCATGTTCCAGTAGAGGTAACGTGCCCATTATGCTTGGATGACTTCGAAGATACGGTCCATGCTTTGTTCTTGTATCGCCGAGCTCATGAAGTTTGGTCTATGCTGGGTTTATGGGAGGAGATTGGCCCAGATCTGATGAGGGAGGCAATTATCTTGCATGTTGATGCGGCATGTGATGATGTTGGTAGGCGTAGTGGGGTGGGTGCCGTTATTCGAGATAAGGAGGGACAGTTGGTGGCTTCTCTTTCAATGGGAACGTCTATTTACTCGGCTCCTTTATGCGCCGAGGCTGTAGCTGTTCTTGAGGGTCTCAAGTTGACAATTCAGCAGAATATTCGTTGTGTGACAGTTTGTTCGGATTCCCTTGCGTTGATCCAAATTCTGCTCCATAGAAGTCGATGTCCTGTAGACTGTGCCAACATTGTTGCGAACATTCATCTTGTAGGGGGATCGTTCCAAACCATCAATTTTTGTCATGTAAATCGTGAGTATAATTTGTTAGCTCATGAATTAGCTCTATTAGGATTATGTTCACCTCTTGTGTTTGGTATAGGGATTTCCCATAGTGGGTTGTTATTCTGA

Protein sequence

MVNLRKHHVPVEVTCPLCLDDFEDTVHALFLYRRAHEVWSMLGLWEEIGPDLMREAIILHVDAACDDVGRRSGVGAVIRDKEGQLVASLSMGTSIYSAPLCAEAVAVLEGLKLTIQQNIRCVTVCSDSLALIQILLHRSRCPVDCANIVANIHLVGGSFQTINFCHVNREYNLLAHELALLGLCSPLVFGIGISHSGLLF

Homology

[Close]

BLAST of Lcy12g017850.1 vs. ExPASy TrEMBL
Match: A0A6J1CNR4 (uncharacterized protein LOC111012950 OS=Momordica charantia OX=3673 GN=LOC111012950 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 4.1e-09
Identity = 52/142 (36.62%), Postives = 78/142 (54.93%), Query Frame = 0


Query: 41  MLGLWEEIGPDLMREAIILHVDAACDDVGRRSGVGAVIRDKEGQLVASLSMGTSIYSAPL 100
           M+GL    G  L+   I ++VD AC     R+ +G V R+++GQ++A+ S     Y  PL
Sbjct: 17  MVGLLRVTGRLLLTGWIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAYHDPL 76

Query: 101 CAEAVAVLEGLKLTIQQNIRCVTVCSDSLALIQILLHRSRCPVD-CANIV--ANIHLVGG 160
            AE++A+LEGL+L    NI+ V   SDSL LI +L   +  P+  C + V   +I ++  
Sbjct: 77  MAESLALLEGLRLADHLNIQQVQFESDSLQLINLL---TEDPISWCGSNVWLEDIRVLAR 136

Query: 161 SFQTINFCHVNREYNLLAHELA 180
           +F   NF HV R  N  A  +A
Sbjct: 137 NFIDFNFLHVCRSSNKAARRVA 155

[Close]

BLAST of Lcy12g017850.1 vs. ExPASy TrEMBL
Match: A0A2P6QT88 (Putative ribonuclease H-like domain, reverse transcriptase zinc-binding domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4g0402201 PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 5.4e-09
Identity = 46/141 (32.62%), Postives = 75/141 (53.19%), Query Frame = 0


Query: 45  WEEIGPDLMREAIILHVDAACDDVGRRSGVGAVIRDKEGQLVASLSMGTSIYSAPLCAEA 104
           WE   P   +    L+ D AC+  G  SG+GAVIR++ G+LV +L++  +    P   EA
Sbjct: 423 WERPPPQWCK----LNFDGACEANGGNSGLGAVIRNELGELVGALAVPFAATLKPSAVEA 482

Query: 105 VAVLEGLKLTIQQNIRCVTVCSDSLALIQILLHRSRCPVDCANIVANIHLVGGSFQTINF 164
           +A+ EGL+ +    IR + V  D+L +I  L        +   ++  + L+   F+ +N+
Sbjct: 483 LALQEGLRYSRLLGIRNLEVEGDALCIINSLSQTENDLTEIGAVLEGVRLLLQEFEFVNW 542

Query: 165 CHVNREYNLLAHELALLGLCS 186
            HV ++ N +AHELA   L S
Sbjct: 543 KHVRKKANQVAHELARSALKS 559

[Close]

BLAST of Lcy12g017850.1 vs. ExPASy TrEMBL
Match: A0A803QP51 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 5.4e-09
Identity = 56/183 (30.60%), Postives = 83/183 (45.36%), Query Frame = 0


Query: 15   CPLCLDDFEDTVHALFLYRRAHEVWSMLGLWEEIGPDLMREA-----------IILHVDA 74
            C LC + +E   HALFLY+ A +VW     +  I       A           + ++VDA
Sbjct: 1073 CSLCSNAWESVEHALFLYKHAKKVWHSAAFYYSIKDSTGAAANSKWFHPPLNELKMNVDA 1132

Query: 75   ACDDVGRRSGVGAVIRDKEGQLVASLSMGTSIYSAPLCA-------EAVAVLEGLKLTIQ 134
            ACD    + GVG +I++  GQ+VA+       YS PL         EA A+L GL    Q
Sbjct: 1133 ACDVSHNKIGVGLIIQNSSGQVVAA-------YSKPLTGRYKSQEIEAKALLTGLDWATQ 1192

Query: 135  QNIRCVTVCSDSLALIQILLHRSRCPVDCANIVANIHLVGGSFQTINFCHVNREYNLLAH 180
             N+    + SDSL L+  +  +S       ++V +I        ++   +V R+ N  AH
Sbjct: 1193 CNLSVNHLESDSLVLVNSINSKSTAISSFGDLVLDIKNRLSYLFSVCVSYVKRDANQAAH 1248

[Close]

BLAST of Lcy12g017850.1 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.6e-08
Identity = 48/128 (37.50%), Postives = 64/128 (50.00%), Query Frame = 0


Query: 59   LHVDAACDDVGRRSGVGAVIRDKEGQLVASLSMGTSIYSAPLCAEAVAVLEGLKLTIQQN 118
            L+ DAAC   G  +  G +IRD    LVA+ S+      +PL AE   +LEGLK     N
Sbjct: 1304 LNTDAACR--GASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCILEGLKFAAASN 1363

Query: 119  IRCVTVCSDSLALIQILLHRSRCPVDCANIVANIHLVGGSFQTINFCHVNREYNLLAHEL 178
               + V SDSL  IQ++ +      D  N V  I  +   F  I+F H +R+ N  AH L
Sbjct: 1364 FTHLEVESDSLLAIQLIRNEIHTRGDEQNWVMEIQALTCCFAFISFSHSSRQCNRAAHGL 1423

Query: 179  ALLGLCSP 187
            A  G+ SP
Sbjct: 1424 AKWGITSP 1429

[Close]

BLAST of Lcy12g017850.1 vs. ExPASy TrEMBL
Match: A0A803NTU1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 2.7e-08
Identity = 46/127 (36.22%), Postives = 69/127 (54.33%), Query Frame = 0


Query: 59   LHVDAACDDVGRRSGVGAVIRDKEGQLVASLSMGTSIYSAPLCAEAVAVLEGLKLTIQQN 118
            ++VDA   ++      G VIRD  GQ++ S S  +S   + L AE+VA++ GLKL +   
Sbjct: 895  VNVDAGVYNMNGSCSSGVVIRDYSGQVLCSSSGFSSRPLSALPAESVAIISGLKLALAAG 954

Query: 119  IRCVTVCSDSLALIQILLHRSRCPVDCANIVANIHLVGGSFQTINFCHVNREYNLLAHEL 178
            ++   V SD L  I ++ + SR   D  N++  I  +  +F  + FC   R+ NLLAH L
Sbjct: 955  VKKNRVASDCLNAINLINNPSRSISDVDNLLEEITGLSCNFDLVEFCFELRDANLLAHSL 1014

Query: 179  ALLGLCS 186
            A L L S
Sbjct: 1015 AKLALKS 1021

[Close]

BLAST of Lcy12g017850.1 vs. NCBI nr
Match: XP_042974784.1 (uncharacterized protein LOC122306423 [Carya illinoinensis])

HSP 1 Score: 77.4 bits (189), Expect = 1.5e-10
Identity = 59/204 (28.92%), Postives = 95/204 (46.57%), Query Frame = 0


Query: 2    VNLRKHHVPVEVTCPLCLDDFEDTVHAL---FLYRR--------------AHEVWSMLGL 61
            +NL+K HV  + TC LC    ED  HAL    LY                  + +  + L
Sbjct: 916  LNLKKRHVLDDATCVLCNQGMEDAAHALRNKQLYENISMPFNVTVNNALALEKEYEQVHL 975

Query: 62   WEEIGPDLMR---------EAIILHVDAACDDVGRRSGVGAVIRDKEGQLVASLSMGTSI 121
            ++   P + +         + + L++D A       +GVG V+R+ +G+++ + S     
Sbjct: 976  FDASNPKISKVVRWHPPPSDFLKLNIDGATFPELSVAGVGVVLRNHKGEVIVACSKVEKE 1035

Query: 122  YSAPLCAEAVAVLEGLKLTIQQNIRCVTVCSDSLALIQILLHRSRCPVDCANIVANIHLV 180
             S+    EAVA+L GL+L +Q  I  + + +D L L+  L   S C  D A I+ +I  +
Sbjct: 1036 VSSAEFIEAVALLRGLQLCVQWGIPKLMLETDCLILVNALNGNSECLTDFAFILQDIRRL 1095

[Close]

BLAST of Lcy12g017850.1 vs. NCBI nr
Match: XP_042950087.1 (uncharacterized protein LOC122282202 [Carya illinoinensis])

HSP 1 Score: 76.6 bits (187), Expect = 2.6e-10
Identity = 60/209 (28.71%), Postives = 90/209 (43.06%), Query Frame = 0


Query: 2    VNLRKHHVPVEVTCPLCLDDFEDTVHALFLYRRAHEVWSML-GLWEEIGPDLM------- 61
            +NL+K HV  + TC LC    ED  HALF       VW +     + + PDL        
Sbjct: 967  LNLKKKHVLDDATCGLCNQGMEDAAHALFFCSEVRSVWGVFCSQMDNMQPDLSFWDLANL 1026

Query: 62   -----------------------REAIILHVDAACDDVGRRSGVGAVIRDKEGQLVASLS 121
                                    + + L++D A       + +G V+RD+ G+++ + S
Sbjct: 1027 AHVQLFDVSNQKITQVVRWHPPPNDFLKLNIDGATFPEHSVARIGVVLRDQYGEVIVACS 1086

Query: 122  MGTSIYSAPLCAEAVAVLEGLKLTIQQNIRCVTVCSDSLALIQILLHRSRCPVDCANIVA 180
                  S+    EAVA+L    L  Q  +  +T+ +D L L+  L   S C  D A I+ 
Sbjct: 1087 KVEKEVSSAEFIEAVALL----LCTQWGVPKITLETDCLVLVNALNENSVCLTDFAFILQ 1146

[Close]

BLAST of Lcy12g017850.1 vs. NCBI nr
Match: XP_042950314.1 (uncharacterized protein LOC122282427 [Carya illinoinensis])

HSP 1 Score: 74.7 bits (182), Expect = 1.0e-09
Identity = 54/189 (28.57%), Postives = 85/189 (44.97%), Query Frame = 0


Query: 1   MVNLRKHHVPVEVTCPLCLDDFEDTVHALFLYRRAHEVW----------SMLGLWEEIGP 60
           +VNLR+  V  E  C  CLD+ ED  HAL +      VW            L  W+    
Sbjct: 496 LVNLRRKKVVEESLCCFCLDNEEDLNHALLICPTNQIVWQKYFPLFKQLQQLTKWQAPPE 555

Query: 61  DLMREAIILHVDAACDDVGRRSGVGAVIRDKEGQLVASLSMGTSIYSAPLCAEAVAVLEG 120
             ++    ++VD A     R++G+G V+RD  G ++ + S            E +A+L G
Sbjct: 556 GFLK----VNVDGAIFADQRKAGIGVVLRDTNGAILMAASKIEMEVDRAATIETIAILRG 615

Query: 121 LKLTIQQNIRCVTVCSDSLALIQILLHRSRCPVDCANIVANIHLVGGSFQTINFCHVNRE 180
           ++L +   I  + + +D L ++Q L           N+ A+I+ +   FQ     HVNR 
Sbjct: 616 IQLCLPLGIPKLIIETDCLHVVQELQSSEASYASAGNLFADINHLMMRFQEAQVIHVNRM 675

[Close]

BLAST of Lcy12g017850.1 vs. NCBI nr
Match: XP_022142956.1 (uncharacterized protein LOC111012950 [Momordica charantia])

HSP 1 Score: 71.6 bits (174), Expect = 8.5e-09
Identity = 52/142 (36.62%), Postives = 78/142 (54.93%), Query Frame = 0


Query: 41  MLGLWEEIGPDLMREAIILHVDAACDDVGRRSGVGAVIRDKEGQLVASLSMGTSIYSAPL 100
           M+GL    G  L+   I ++VD AC     R+ +G V R+++GQ++A+ S     Y  PL
Sbjct: 17  MVGLLRVTGRLLLTGWIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAYHDPL 76

Query: 101 CAEAVAVLEGLKLTIQQNIRCVTVCSDSLALIQILLHRSRCPVD-CANIV--ANIHLVGG 160
            AE++A+LEGL+L    NI+ V   SDSL LI +L   +  P+  C + V   +I ++  
Sbjct: 77  MAESLALLEGLRLADHLNIQQVQFESDSLQLINLL---TEDPISWCGSNVWLEDIRVLAR 136

Query: 161 SFQTINFCHVNREYNLLAHELA 180
           +F   NF HV R  N  A  +A
Sbjct: 137 NFIDFNFLHVCRSSNKAARRVA 155

[Close]

BLAST of Lcy12g017850.1 vs. NCBI nr
Match: PRQ37402.1 (putative ribonuclease H-like domain, reverse transcriptase zinc-binding domain-containing protein [Rosa chinensis])

HSP 1 Score: 71.2 bits (173), Expect = 1.1e-08
Identity = 46/141 (32.62%), Postives = 75/141 (53.19%), Query Frame = 0


Query: 45  WEEIGPDLMREAIILHVDAACDDVGRRSGVGAVIRDKEGQLVASLSMGTSIYSAPLCAEA 104
           WE   P   +    L+ D AC+  G  SG+GAVIR++ G+LV +L++  +    P   EA
Sbjct: 423 WERPPPQWCK----LNFDGACEANGGNSGLGAVIRNELGELVGALAVPFAATLKPSAVEA 482

Query: 105 VAVLEGLKLTIQQNIRCVTVCSDSLALIQILLHRSRCPVDCANIVANIHLVGGSFQTINF 164
           +A+ EGL+ +    IR + V  D+L +I  L        +   ++  + L+   F+ +N+
Sbjct: 483 LALQEGLRYSRLLGIRNLEVEGDALCIINSLSQTENDLTEIGAVLEGVRLLLQEFEFVNW 542

Query: 165 CHVNREYNLLAHELALLGLCS 186
            HV ++ N +AHELA   L S
Sbjct: 543 KHVRKKANQVAHELARSALKS 559

The following BLAST results are available for this feature:

BLAST of Lcy12g017850.1 vs.


Match Name	E-value	Identity	Description

BLAST of Lcy12g017850.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CNR4	4.1e-09	36.62	uncharacterized protein LOC111012950 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A2P6QT88	5.4e-09	32.62	Putative ribonuclease H-like domain, reverse transcriptase zinc-binding domain-c...	[more]
A0A803QP51	5.4e-09	30.60	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A6J1DX30	1.6e-08	37.50	uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A803NTU1	2.7e-08	36.22	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]

BLAST of Lcy12g017850.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_042974784.1	1.5e-10	28.92	uncharacterized protein LOC122306423 [Carya illinoinensis]	[more]
XP_042950087.1	2.6e-10	28.71	uncharacterized protein LOC122282202 [Carya illinoinensis]	[more]
XP_042950314.1	1.0e-09	28.57	uncharacterized protein LOC122282427 [Carya illinoinensis]	[more]
XP_022142956.1	8.5e-09	36.62	uncharacterized protein LOC111012950 [Momordica charantia]	[more]
PRQ37402.1	1.1e-08	32.62	putative ribonuclease H-like domain, reverse transcriptase zinc-binding domain-c...	[more]

BLAST of Lcy12g017850.1 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 61..179 e-value: 2.7E-18 score: 66.0
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 56..185 e-value: 3.4E-16 score: 61.4
None	No IPR available	PANTHER	PTHR47723	OS05G0353850 PROTEIN	coord: 59..185
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 59..179 e-value: 2.93646E-18 score: 75.0432
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 57..186

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lcy12g017850	Lcy12g017850	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy12g017850.1.exon2	Lcy12g017850.1.exon2	exon
Lcy12g017850.1.exon1	Lcy12g017850.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy12g017850.1.cds2	Lcy12g017850.1.cds2	CDS
Lcy12g017850.1.cds1	Lcy12g017850.1.cds1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy12g017850.1.utr5p1	Lcy12g017850.1.utr5p1	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lcy12g017850.1	Lcy12g017850.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity