Lcy09g011440.1 (mRNA) Sponge gourd (P93075) v1

Overview


Name	Lcy09g011440.1
Type	mRNA
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	E3 ubiquitin ligase BIG BROTHER-related
Location	Chr09: 12469318 .. 12469947 (+)
Sequence length	630
RNA-Seq Expression	Lcy09g011440.1
Synteny	Lcy09g011440.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGAGCAAGAGGATAAGCAACTTCCCCAAAGGATTTCCCTTGCTTATGTGGAACAGGTGAACTCAGATTTTATCATGGCCTTGGCAATGCAGCAGCAGGACCACGAGAGGGCCTATACAATGCTCGAGACCATTGAAAGTGACAGCGACGAAGAAGAAAGTTCAGATTCCAACAGCAACAATGGTCTCGACACCTATGATTTCTTGCAAAGCCAAGAACTTCGTTGTGCCCGGTGGGATTTTCTGGCAGACGATGAGGAGACTAACGATGAAAATGAAGATATGGACGAAGACGAAGGGGATTTTGAGGATTTTGATTTGGATGAGCTAAGCTATGAAGAGTTGATTGCATTAGGGGAGTTCATTGGAGAAGAAAAGAGGGGGCTACCGATGAACGAAATACCTTCGTGCTTGCACTCGAGCAAGTTTCGAACAATCGAAAACAGAAGCGGTATAGATCGGTGTGTGATTTGCCAAGTTGAATATGAAGATGGTGAAGAATTGGCTTCTCTTCCTTGTGAGCATCCTTACCATTCAGAGTGTATAAGCAAATGGCTTCAAGTCAAAAGGGTTTGTCCAATATGTGGTACAGAAGTTTCATCCCCAAAGGCTTCTAAAAATGCATAA

mRNA sequence

ATGGAAGAGCAAGAGGATAAGCAACTTCCCCAAAGGATTTCCCTTGCTTATGTGGAACAGGTGAACTCAGATTTTATCATGGCCTTGGCAATGCAGCAGCAGGACCACGAGAGGGCCTATACAATGCTCGAGACCATTGAAAGTGACAGCGACGAAGAAGAAAGTTCAGATTCCAACAGCAACAATGGTCTCGACACCTATGATTTCTTGCAAAGCCAAGAACTTCGTTGTGCCCGGTGGGATTTTCTGGCAGACGATGAGGAGACTAACGATGAAAATGAAGATATGGACGAAGACGAAGGGGATTTTGAGGATTTTGATTTGGATGAGCTAAGCTATGAAGAGTTGATTGCATTAGGGGAGTTCATTGGAGAAGAAAAGAGGGGGCTACCGATGAACGAAATACCTTCGTGCTTGCACTCGAGCAAGTTTCGAACAATCGAAAACAGAAGCGGTATAGATCGGTGTGTGATTTGCCAAGTTGAATATGAAGATGGTGAAGAATTGGCTTCTCTTCCTTGTGAGCATCCTTACCATTCAGAGTGTATAAGCAAATGGCTTCAAGTCAAAAGGGTTTGTCCAATATGTGGTACAGAAGTTTCATCCCCAAAGGCTTCTAAAAATGCATAA

Coding sequence (CDS)

ATGGAAGAGCAAGAGGATAAGCAACTTCCCCAAAGGATTTCCCTTGCTTATGTGGAACAGGTGAACTCAGATTTTATCATGGCCTTGGCAATGCAGCAGCAGGACCACGAGAGGGCCTATACAATGCTCGAGACCATTGAAAGTGACAGCGACGAAGAAGAAAGTTCAGATTCCAACAGCAACAATGGTCTCGACACCTATGATTTCTTGCAAAGCCAAGAACTTCGTTGTGCCCGGTGGGATTTTCTGGCAGACGATGAGGAGACTAACGATGAAAATGAAGATATGGACGAAGACGAAGGGGATTTTGAGGATTTTGATTTGGATGAGCTAAGCTATGAAGAGTTGATTGCATTAGGGGAGTTCATTGGAGAAGAAAAGAGGGGGCTACCGATGAACGAAATACCTTCGTGCTTGCACTCGAGCAAGTTTCGAACAATCGAAAACAGAAGCGGTATAGATCGGTGTGTGATTTGCCAAGTTGAATATGAAGATGGTGAAGAATTGGCTTCTCTTCCTTGTGAGCATCCTTACCATTCAGAGTGTATAAGCAAATGGCTTCAAGTCAAAAGGGTTTGTCCAATATGTGGTACAGAAGTTTCATCCCCAAAGGCTTCTAAAAATGCATAA

Protein sequence

MEEQEDKQLPQRISLAYVEQVNSDFIMALAMQQQDHERAYTMLETIESDSDEEESSDSNSNNGLDTYDFLQSQELRCARWDFLADDEETNDENEDMDEDEGDFEDFDLDELSYEELIALGEFIGEEKRGLPMNEIPSCLHSSKFRTIENRSGIDRCVICQVEYEDGEELASLPCEHPYHSECISKWLQVKRVCPICGTEVSSPKASKNA

Homology

[Close]

BLAST of Lcy09g011440.1 vs. ExPASy Swiss-Prot
Match: Q9LT17 (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.5e-22
Identity = 83/259 (32.05%), Postives = 126/259 (48.65%), Query Frame = 0


Query: 12  RISLAYVEQVNSDFIMALAMQQQDHERAYTML---------------------------- 71
           R+    + Q+++D  +ALA   Q+ ERAY ML                            
Sbjct: 85  RVPFTNLSQIDAD--LALARTLQEQERAYMMLTMNSEISDYGSWETGSYVYDEDEFDDPE 144

Query: 72  -ETIESDSDEEESSDSNSNNGLD-----------------------TYD-----FLQSQE 131
            E  + D DE E+ D    +GLD                       T D      LQ  E
Sbjct: 145 NEDEDDDEDEYETDDDPQEDGLDVNVHANEDDQEDDGNSDIEEVAYTDDEAYARALQEAE 204

Query: 132 LR--CARWDFLAD--DEETNDENEDMDEDEGDFEDFDLDELSYEELIALGEFIGEEKRGL 191
            R   AR   L+   +    D  ++    +  +++ D DELSYEEL+ALG+ +G E RGL
Sbjct: 205 ERDMAARLSALSGLANRVVEDLEDESHTSQDAWDEMDPDELSYEELLALGDIVGTESRGL 264

Query: 192 PMNEIPSCLHSSKFRTIENRSGI-DRCVICQVEYEDGEELASLPCEHPYHSECISKWLQV 209
             + I S L S +++  +N++G  + CVIC+++YED E+L  LPC+H YHSECI+ WL++
Sbjct: 265 SADTIAS-LPSKRYKEGDNQNGTNESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKI 324

[Close]

BLAST of Lcy09g011440.1 vs. ExPASy Swiss-Prot
Match: Q8L649 (E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.7e-18
Identity = 68/202 (33.66%), Postives = 100/202 (49.50%), Query Frame = 0


Query: 28  ALAMQQQDHERAYTMLETIESDSDEEESSDSNSNNGLDTYDFLQSQELRCARWDF----- 87
           A  M  QD+   Y  + T         S +++     D  D L    +    WD+     
Sbjct: 43  AATMHPQDN--LYWTMNTNAYKFGFSGSDNASFYGSYDMNDHLSRMSIGRTNWDYHPMVN 102

Query: 88  LADDEET----------NDENEDMDE---DEGD--------FEDFDLDELSYEELIALGE 147
           +ADD E            DE+ + +E   +E D         +D D D ++YEEL+ LGE
Sbjct: 103 VADDPENTVARSVQIGDTDEHSEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGE 162

Query: 148 FIGEEKRGLPMNEIPSCLHSSKFR---TIENRSGIDRCVICQVEYEDGEELASLPCEHPY 201
            +G E RGL   E+   L + K++       +   +RCVICQ++Y+ GE   +LPC+H Y
Sbjct: 163 AVGTESRGL-SQELIETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVY 222

[Close]

BLAST of Lcy09g011440.1 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.1e-17
Identity = 42/98 (42.86%), Postives = 59/98 (60.20%), Query Frame = 0


Query: 107 DLDELSYEELIALGEFIGEEKRGLPMNEIPSCLH-----SSKFRTIENRSGIDRCVICQV 166
           D+D +SYEEL+ALGE IG+   GL    I   +      SS   ++E    I+ C ICQ 
Sbjct: 602 DVDNMSYEELLALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIEPCCICQE 661

Query: 167 EYEDGEELASLPCEHPYHSECISKWLQVKRVCPICGTE 200
           EY +G+ L +L C H +H +CI +W+ +K +CPIC TE
Sbjct: 662 EYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

[Close]

BLAST of Lcy09g011440.1 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 87.8 bits (216), Expect = 1.6e-16
Identity = 40/92 (43.48%), Postives = 54/92 (58.70%), Query Frame = 0


Query: 107 DLDELSYEELIALGEFIGEEKRGLPMNEIPSCLHSSKFRTIENRSGIDR--CVICQVEYE 166
           D+D +SYEEL+AL E IG    GL   E+   L   KF +    + ++   C ICQ EY 
Sbjct: 569 DIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRLEASVEEEPCCICQEEYV 628

Query: 167 DGEELASLPCEHPYHSECISKWLQVKRVCPIC 197
           DG++L +L C H +H  C+ +WL VK  CPIC
Sbjct: 629 DGDDLGTLDCGHDFHVGCVRQWLVVKNTCPIC 660

[Close]

BLAST of Lcy09g011440.1 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 3.0e-15
Identity = 48/143 (33.57%), Postives = 77/143 (53.85%), Query Frame = 0


Query: 57  DSNSNNGLDTYDFLQSQELRCARWDFLADDEETNDENEDMDEDEGDFEDFDLDELSYEEL 116
           D+N+NN       L+ +++       L D    +D   DM          D+D +SYEEL
Sbjct: 552 DANNNN-------LRLEDVMLLNHSVLFDGATGHDRYRDM--------RLDVDNMSYEEL 611

Query: 117 IALGEFIGEEKRGLPMNEIPSCLHSSKFRT-IENRSGIDRCVICQVEYEDGEELASLPCE 176
           +AL E IG+   G+    I + L   K+++  ++    + C +CQ EY +GE++ +L C 
Sbjct: 612 LALEERIGDVCTGVNEETISNRLKQRKYKSNTKSPQDAEPCCVCQEEYTEGEDMGTLECG 671

Query: 177 HPYHSECISKWLQVKRVCPICGT 199
           H +HS+CI +WL+ K +CPIC T
Sbjct: 672 HEFHSQCIKEWLKQKNLCPICKT 679

[Close]

BLAST of Lcy09g011440.1 vs. ExPASy TrEMBL
Match: A0A6J1CJH0 (E3 ubiquitin ligase BIG BROTHER-related OS=Momordica charantia OX=3673 GN=LOC111011883 PE=4 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 4.3e-94
Identity = 179/205 (87.32%), Postives = 191/205 (93.17%), Query Frame = 0


Query: 1   MEEQEDKQLPQRISLAYVEQVNSDFIMALAMQQQDHERAYTMLETIESDSDEEESSDSNS 60
           MEEQEDKQ PQRISLAYVEQVNSDFIMALAMQQQ+HERAYTMLETIESDSDE+ES DSNS
Sbjct: 1   MEEQEDKQPPQRISLAYVEQVNSDFIMALAMQQQEHERAYTMLETIESDSDEDESFDSNS 60

Query: 61  NNGLDTYDFLQSQELRCARWDFLADDEETNDENEDMDEDEGDFEDFDLDELSYEELIALG 120
           N GLDTYD LQSQE   +RWDFLADDEET DE+ D D DE + +DFDLDEL+YEEL+ALG
Sbjct: 61  NTGLDTYDPLQSQEFH-SRWDFLADDEETTDEDMDEDVDEDELDDFDLDELTYEELVALG 120

Query: 121 EFIGEEKRGLPMNEIPSCLHSSKFRTIENRSGIDRCVICQVEYEDGEELASLPCEHPYHS 180
           EFIGEEKRGLPMNEIPSCLHS KFRTIEN+SG+DRCVICQVEYEDGEELA+LPCEHPYHS
Sbjct: 121 EFIGEEKRGLPMNEIPSCLHSGKFRTIENKSGMDRCVICQVEYEDGEELAALPCEHPYHS 180

Query: 181 ECISKWLQVKRVCPICGTEVSSPKA 206
           ECISKWLQ+KRVCPICGTEVSSPKA
Sbjct: 181 ECISKWLQIKRVCPICGTEVSSPKA 204

[Close]

BLAST of Lcy09g011440.1 vs. ExPASy TrEMBL
Match: A0A6J1GTM7 (E3 ubiquitin ligase BIG BROTHER-related OS=Cucurbita moschata OX=3662 GN=LOC111457005 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 6.5e-90
Identity = 181/212 (85.38%), Postives = 193/212 (91.04%), Query Frame = 0


Query: 1   MEEQEDKQLPQRISLAYVEQVNSDFIMALAMQQQDHERAYTMLETIESDSDEEESSDSNS 60
           MEEQEDKQLPQRIS AYVEQVN+DFIMALAMQQQ+HER YTMLETIESDS+E+E SDSNS
Sbjct: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60

Query: 61  NNGLD-TYDFLQSQELRCARWDFLADD-EETNDENEDMDED-EGDFEDFDLDELSYEELI 120
           +NGLD T D  QSQEL  + W FLADD EET D+NE MDED E D E+FDLDELSYEELI
Sbjct: 61  SNGLDHTNDSFQSQELH-SPWGFLADDEEETTDDNEYMDEDEEEDIEEFDLDELSYEELI 120

Query: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFRTIENRSGIDRCVICQVEYEDGEELASLPCEHP 180
           ALGEFIGEEKRGLPMNEIPSCLHSSKF+TIEN+SGIDRCVICQVEYED EELA+LPCEHP
Sbjct: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP 180

Query: 181 YHSECISKWLQVKRVCPICGTEVSSPKASKNA 210
           YHSECISKWLQ+KRVCPICGTEVSSPK SKNA
Sbjct: 181 YHSECISKWLQIKRVCPICGTEVSSPKPSKNA 211

[Close]

BLAST of Lcy09g011440.1 vs. ExPASy TrEMBL
Match: A0A6J1JR32 (E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucurbita maxima OX=3661 GN=LOC111487582 PE=4 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 1.1e-89
Identity = 181/212 (85.38%), Postives = 194/212 (91.51%), Query Frame = 0


Query: 1   MEEQEDKQLPQRISLAYVEQVNSDFIMALAMQQQDHERAYTMLETIESDSDEEESSDSNS 60
           MEEQEDKQLPQRIS AYVEQVN+DFIMALAMQQQ+HER YTMLETIESDS+E+E SDSNS
Sbjct: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60

Query: 61  NNGLD-TYDFLQSQELRCARWDFLADD-EETNDENEDMDED-EGDFEDFDLDELSYEELI 120
           +NGLD T D LQSQEL  + W FLADD EET D+NE MDED E D E+FDLDELSYEELI
Sbjct: 61  SNGLDHTNDSLQSQELH-SPWGFLADDEEETTDDNEYMDEDEEEDIEEFDLDELSYEELI 120

Query: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFRTIENRSGIDRCVICQVEYEDGEELASLPCEHP 180
           ALGEFIGEEKRGLPMNEIPSCLHSSKF+TIEN+SGIDRCVICQVEYED EELA+LPCEHP
Sbjct: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP 180

Query: 181 YHSECISKWLQVKRVCPICGTEVSSPKASKNA 210
           YHSECISKWLQ+KRVCPICGTEVSS K+SKNA
Sbjct: 181 YHSECISKWLQIKRVCPICGTEVSSSKSSKNA 211

[Close]

BLAST of Lcy09g011440.1 vs. ExPASy TrEMBL
Match: A0A0A0KFS4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G382920 PE=4 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 1.1e-86
Identity = 173/217 (79.72%), Postives = 188/217 (86.64%), Query Frame = 0


Query: 1   MEEQEDK--------QLPQRISLAYVEQVNSDFIMALAMQQQDHERAYTMLETIESDSDE 60
           MEEQED         QLPQRISLAYVEQVN+D IMALAMQQQ+HE AYT LETI SDS+E
Sbjct: 1   MEEQEDNKQQQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHELAYTTLETIASDSEE 60

Query: 61  EESSDSNSNNGLDTYDFLQSQELRCARWDFLADDEETNDENEDMDEDE-GDFEDFDLDEL 120
           +E+SDSNSNNGLDT    Q QEL  +RW FL DDEET D N+DM+EDE GDFE FDLDEL
Sbjct: 61  DENSDSNSNNGLDTNATSQRQEL-VSRWAFLEDDEETTDGNDDMEEDEDGDFEGFDLDEL 120

Query: 121 SYEELIALGEFIGEEKRGLPMNEIPSCLHSSKFRTIENRSGIDRCVICQVEYEDGEELAS 180
           +YEELIALGEFIGEEKRGLP+NEIPSCLHSSKF+TIENRSGIDRCVICQVEY+DGEELA+
Sbjct: 121 TYEELIALGEFIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQVEYDDGEELAA 180

Query: 181 LPCEHPYHSECISKWLQVKRVCPICGTEVSSPKASKN 209
           LPCEHPYHSECI +WLQ+KRVCPICGTEVSSP  SKN
Sbjct: 181 LPCEHPYHSECIGEWLQIKRVCPICGTEVSSPNGSKN 216

[Close]

BLAST of Lcy09g011440.1 vs. ExPASy TrEMBL
Match: A0A6J1K3Q9 (E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucurbita maxima OX=3661 GN=LOC111489783 PE=4 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 2.2e-85
Identity = 175/211 (82.94%), Postives = 183/211 (86.73%), Query Frame = 0


Query: 1   MEEQEDK-QLPQRISLAYVEQVNSDFIMALAMQQQDHERAYTMLETIESDSDEEESSDSN 60
           M EQEDK QLPQRISL YVEQVNSD IMALAMQQQ+HE AYT L TIESDSDE+E SDSN
Sbjct: 1   MGEQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDSDEDERSDSN 60

Query: 61  SNNGLDTYDFLQSQELRCARWDFLADDEETNDENEDMDE-DEGDFEDFDLDELSYEELIA 120
           SNNGLDT   LQSQ        FLAD+EET DENEDMDE DE  FEDFD DEL+YEELIA
Sbjct: 61  SNNGLDTNASLQSQ--------FLADNEETTDENEDMDEDDEEGFEDFDFDELTYEELIA 120

Query: 121 LGEFIGEEKRGLPMNEIPSCLHSSKFRTIENRSGIDRCVICQVEYEDGEELASLPCEHPY 180
           LGEFIGEEKRGLP NEIPSCLHSSKFR+IEN SGIDRCVICQVEYEDGEELA+LPCEHPY
Sbjct: 121 LGEFIGEEKRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAALPCEHPY 180

Query: 181 HSECISKWLQVKRVCPICGTEVSSPKASKNA 210
           HS+CI KW+Q KRVCPICGTEVSSPKASKNA
Sbjct: 181 HSDCIVKWVQTKRVCPICGTEVSSPKASKNA 203

[Close]

BLAST of Lcy09g011440.1 vs. NCBI nr
Match: XP_022141536.1 (E3 ubiquitin ligase BIG BROTHER-related [Momordica charantia])

HSP 1 Score: 354.0 bits (907), Expect = 9.0e-94
Identity = 179/205 (87.32%), Postives = 191/205 (93.17%), Query Frame = 0


Query: 1   MEEQEDKQLPQRISLAYVEQVNSDFIMALAMQQQDHERAYTMLETIESDSDEEESSDSNS 60
           MEEQEDKQ PQRISLAYVEQVNSDFIMALAMQQQ+HERAYTMLETIESDSDE+ES DSNS
Sbjct: 1   MEEQEDKQPPQRISLAYVEQVNSDFIMALAMQQQEHERAYTMLETIESDSDEDESFDSNS 60

Query: 61  NNGLDTYDFLQSQELRCARWDFLADDEETNDENEDMDEDEGDFEDFDLDELSYEELIALG 120
           N GLDTYD LQSQE   +RWDFLADDEET DE+ D D DE + +DFDLDEL+YEEL+ALG
Sbjct: 61  NTGLDTYDPLQSQEFH-SRWDFLADDEETTDEDMDEDVDEDELDDFDLDELTYEELVALG 120

Query: 121 EFIGEEKRGLPMNEIPSCLHSSKFRTIENRSGIDRCVICQVEYEDGEELASLPCEHPYHS 180
           EFIGEEKRGLPMNEIPSCLHS KFRTIEN+SG+DRCVICQVEYEDGEELA+LPCEHPYHS
Sbjct: 121 EFIGEEKRGLPMNEIPSCLHSGKFRTIENKSGMDRCVICQVEYEDGEELAALPCEHPYHS 180

Query: 181 ECISKWLQVKRVCPICGTEVSSPKA 206
           ECISKWLQ+KRVCPICGTEVSSPKA
Sbjct: 181 ECISKWLQIKRVCPICGTEVSSPKA 204

[Close]

BLAST of Lcy09g011440.1 vs. NCBI nr
Match: XP_038886321.1 (E3 ubiquitin ligase BIG BROTHER-related-like [Benincasa hispida] >XP_038886322.1 E3 ubiquitin ligase BIG BROTHER-related-like [Benincasa hispida] >XP_038886323.1 E3 ubiquitin ligase BIG BROTHER-related-like [Benincasa hispida])

HSP 1 Score: 340.5 bits (872), Expect = 1.0e-89
Identity = 178/211 (84.36%), Postives = 193/211 (91.47%), Query Frame = 0


Query: 1   MEEQEDK-QLPQRISLAYVEQVNSDFIMALAMQQQDHERAYTMLETIESDSDEEESSDSN 60
           MEEQEDK QLPQRISLA+VEQVNSD IMALAMQQQ+HE AYTMLETIESDSDE+E+SDSN
Sbjct: 1   MEEQEDKQQLPQRISLAHVEQVNSDLIMALAMQQQEHEMAYTMLETIESDSDEDENSDSN 60

Query: 61  SNNGLDTYDFLQSQELRCARWDFLADDEETNDENEDMDEDE-GDFEDFDLDELSYEELIA 120
           SN+GL+T    Q QEL  +RW FL DDEET DENEDM+EDE GDFEDFDLDEL+YEELIA
Sbjct: 61  SNHGLNTNASSQRQEL-LSRWAFLEDDEETADENEDMEEDEDGDFEDFDLDELTYEELIA 120

Query: 121 LGEFIGEEKRGLPMNEIPSCLHSSKFRTIENRSGIDRCVICQVEYEDGEELASLPCEHPY 180
           LGEFIG EKRGLP+NEIPSCLH SKF+TIENRSGIDRCVICQVEY++GEELA+LPCEHPY
Sbjct: 121 LGEFIGTEKRGLPINEIPSCLHPSKFQTIENRSGIDRCVICQVEYDEGEELAALPCEHPY 180

Query: 181 HSECISKWLQVKRVCPICGTEVSSPKASKNA 210
           HSECI KWLQ+KRVCPICGTEVSSPK SKNA
Sbjct: 181 HSECIGKWLQIKRVCPICGTEVSSPKGSKNA 210

[Close]

BLAST of Lcy09g011440.1 vs. NCBI nr
Match: XP_022954880.1 (E3 ubiquitin ligase BIG BROTHER-related [Cucurbita moschata])

HSP 1 Score: 340.1 bits (871), Expect = 1.3e-89
Identity = 181/212 (85.38%), Postives = 193/212 (91.04%), Query Frame = 0


Query: 1   MEEQEDKQLPQRISLAYVEQVNSDFIMALAMQQQDHERAYTMLETIESDSDEEESSDSNS 60
           MEEQEDKQLPQRIS AYVEQVN+DFIMALAMQQQ+HER YTMLETIESDS+E+E SDSNS
Sbjct: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60

Query: 61  NNGLD-TYDFLQSQELRCARWDFLADD-EETNDENEDMDED-EGDFEDFDLDELSYEELI 120
           +NGLD T D  QSQEL  + W FLADD EET D+NE MDED E D E+FDLDELSYEELI
Sbjct: 61  SNGLDHTNDSFQSQELH-SPWGFLADDEEETTDDNEYMDEDEEEDIEEFDLDELSYEELI 120

Query: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFRTIENRSGIDRCVICQVEYEDGEELASLPCEHP 180
           ALGEFIGEEKRGLPMNEIPSCLHSSKF+TIEN+SGIDRCVICQVEYED EELA+LPCEHP
Sbjct: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP 180

Query: 181 YHSECISKWLQVKRVCPICGTEVSSPKASKNA 210
           YHSECISKWLQ+KRVCPICGTEVSSPK SKNA
Sbjct: 181 YHSECISKWLQIKRVCPICGTEVSSPKPSKNA 211

[Close]

BLAST of Lcy09g011440.1 vs. NCBI nr
Match: XP_022990800.1 (E3 ubiquitin ligase BIG BROTHER-related-like [Cucurbita maxima])

HSP 1 Score: 339.3 bits (869), Expect = 2.3e-89
Identity = 181/212 (85.38%), Postives = 194/212 (91.51%), Query Frame = 0


Query: 1   MEEQEDKQLPQRISLAYVEQVNSDFIMALAMQQQDHERAYTMLETIESDSDEEESSDSNS 60
           MEEQEDKQLPQRIS AYVEQVN+DFIMALAMQQQ+HER YTMLETIESDS+E+E SDSNS
Sbjct: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60

Query: 61  NNGLD-TYDFLQSQELRCARWDFLADD-EETNDENEDMDED-EGDFEDFDLDELSYEELI 120
           +NGLD T D LQSQEL  + W FLADD EET D+NE MDED E D E+FDLDELSYEELI
Sbjct: 61  SNGLDHTNDSLQSQELH-SPWGFLADDEEETTDDNEYMDEDEEEDIEEFDLDELSYEELI 120

Query: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFRTIENRSGIDRCVICQVEYEDGEELASLPCEHP 180
           ALGEFIGEEKRGLPMNEIPSCLHSSKF+TIEN+SGIDRCVICQVEYED EELA+LPCEHP
Sbjct: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP 180

Query: 181 YHSECISKWLQVKRVCPICGTEVSSPKASKNA 210
           YHSECISKWLQ+KRVCPICGTEVSS K+SKNA
Sbjct: 181 YHSECISKWLQIKRVCPICGTEVSSSKSSKNA 211

[Close]

BLAST of Lcy09g011440.1 vs. NCBI nr
Match: KAG7032814.1 (E3 ubiquitin ligase BIG BROTHER-related, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 338.2 bits (866), Expect = 5.1e-89
Identity = 180/213 (84.51%), Postives = 193/213 (90.61%), Query Frame = 0


Query: 1   MEEQEDKQLPQRISLAYVEQVNSDFIMALAMQQQDHERAYTMLETIESDSDEEESSDSNS 60
           MEEQEDKQLPQRIS AYVEQVN+DFIMALAMQQQ+HER YTMLETIESDS+E+E SDSNS
Sbjct: 1   MEEQEDKQLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSNS 60

Query: 61  NNGLD-TYDFLQSQELRCARWDFLAD--DEETNDENEDMDED-EGDFEDFDLDELSYEEL 120
           +NGLD T D  QSQEL  + W FLAD  +EET D+NE MDED E D E+FDLDELSYEEL
Sbjct: 61  SNGLDHTNDSFQSQELH-SPWGFLADEEEEETTDDNEYMDEDAEEDIEEFDLDELSYEEL 120

Query: 121 IALGEFIGEEKRGLPMNEIPSCLHSSKFRTIENRSGIDRCVICQVEYEDGEELASLPCEH 180
           IALGEFIGEEKRGLPMNEIPSCLHSSKF+TIEN+SGIDRCVICQVEYED EELA+LPCEH
Sbjct: 121 IALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEH 180

Query: 181 PYHSECISKWLQVKRVCPICGTEVSSPKASKNA 210
           PYHSECISKWLQ+KRVCPICGTEVSSPK SKNA
Sbjct: 181 PYHSECISKWLQIKRVCPICGTEVSSPKPSKNA 212

[Close]

BLAST of Lcy09g011440.1 vs. TAIR 10
Match: AT3G47180.1 (RING/U-box superfamily protein )

HSP 1 Score: 145.2 bits (365), Expect = 5.9e-35
Identity = 90/216 (41.67%), Postives = 131/216 (60.65%), Query Frame = 0


Query: 2   EEQEDKQLPQRI-SLAYVEQVNSDFIMALAMQQQD-----HERAYTMLETIESDSDEEES 61
           +E+E KQ P ++  LA  EQ NS+  +A +          H+ + +M+  IESD + E+ 
Sbjct: 4   QEEEPKQPPNKLPDLALFEQANSEVALAASQANSHFAHAMHDSSPSMISMIESDEESEDE 63

Query: 62  SDSNSNNGLDTYDFLQSQELRCARWDFLADDEETNDENEDMD-----EDEGDF----EDF 121
            + N N     Y++  S         F  D++E N+  ED +     E+E DF    ++ 
Sbjct: 64  EEINEN----YYEYFDSN-------GFGVDEDEINEFLEDQESNSNLEEEDDFLEEEDEI 123

Query: 122 DLDELSYEELIALGEFIGEEKRGLPMNEIPSCLHSSKFRTIENRSGIDRCVICQVEYEDG 181
           D D+LSYEELIALG+FIG E RGL   EI +CL++S +    N++ IDRCV+CQ+E+E+ 
Sbjct: 124 DPDQLSYEELIALGDFIGVENRGLTPIEISTCLNASTYVFSHNKNEIDRCVVCQMEFEER 183

Query: 182 EELASL-PCEHPYHSECISKWLQVKRVCPICGTEVS 202
           E L  L PC+HPYHSECI+KWL+ K++CPIC +E S
Sbjct: 184 ESLVVLRPCDHPYHSECITKWLETKKICPICCSEPS 208

[Close]

BLAST of Lcy09g011440.1 vs. TAIR 10
Match: AT3G19910.1 (RING/U-box superfamily protein )

HSP 1 Score: 107.8 bits (268), Expect = 1.0e-23
Identity = 83/259 (32.05%), Postives = 126/259 (48.65%), Query Frame = 0


Query: 12  RISLAYVEQVNSDFIMALAMQQQDHERAYTML---------------------------- 71
           R+    + Q+++D  +ALA   Q+ ERAY ML                            
Sbjct: 85  RVPFTNLSQIDAD--LALARTLQEQERAYMMLTMNSEISDYGSWETGSYVYDEDEFDDPE 144

Query: 72  -ETIESDSDEEESSDSNSNNGLD-----------------------TYD-----FLQSQE 131
            E  + D DE E+ D    +GLD                       T D      LQ  E
Sbjct: 145 NEDEDDDEDEYETDDDPQEDGLDVNVHANEDDQEDDGNSDIEEVAYTDDEAYARALQEAE 204

Query: 132 LR--CARWDFLAD--DEETNDENEDMDEDEGDFEDFDLDELSYEELIALGEFIGEEKRGL 191
            R   AR   L+   +    D  ++    +  +++ D DELSYEEL+ALG+ +G E RGL
Sbjct: 205 ERDMAARLSALSGLANRVVEDLEDESHTSQDAWDEMDPDELSYEELLALGDIVGTESRGL 264

Query: 192 PMNEIPSCLHSSKFRTIENRSGI-DRCVICQVEYEDGEELASLPCEHPYHSECISKWLQV 209
             + I S L S +++  +N++G  + CVIC+++YED E+L  LPC+H YHSECI+ WL++
Sbjct: 265 SADTIAS-LPSKRYKEGDNQNGTNESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKI 324

[Close]

BLAST of Lcy09g011440.1 vs. TAIR 10
Match: AT3G63530.1 (RING/U-box superfamily protein )

HSP 1 Score: 94.4 bits (233), Expect = 1.2e-19
Identity = 68/202 (33.66%), Postives = 100/202 (49.50%), Query Frame = 0


Query: 28  ALAMQQQDHERAYTMLETIESDSDEEESSDSNSNNGLDTYDFLQSQELRCARWDF----- 87
           A  M  QD+   Y  + T         S +++     D  D L    +    WD+     
Sbjct: 43  AATMHPQDN--LYWTMNTNAYKFGFSGSDNASFYGSYDMNDHLSRMSIGRTNWDYHPMVN 102

Query: 88  LADDEET----------NDENEDMDE---DEGD--------FEDFDLDELSYEELIALGE 147
           +ADD E            DE+ + +E   +E D         +D D D ++YEEL+ LGE
Sbjct: 103 VADDPENTVARSVQIGDTDEHSEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGE 162

Query: 148 FIGEEKRGLPMNEIPSCLHSSKFR---TIENRSGIDRCVICQVEYEDGEELASLPCEHPY 201
            +G E RGL   E+   L + K++       +   +RCVICQ++Y+ GE   +LPC+H Y
Sbjct: 163 AVGTESRGL-SQELIETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVY 222

[Close]

BLAST of Lcy09g011440.1 vs. TAIR 10
Match: AT3G63530.2 (RING/U-box superfamily protein )

HSP 1 Score: 94.4 bits (233), Expect = 1.2e-19
Identity = 68/202 (33.66%), Postives = 100/202 (49.50%), Query Frame = 0


Query: 28  ALAMQQQDHERAYTMLETIESDSDEEESSDSNSNNGLDTYDFLQSQELRCARWDF----- 87
           A  M  QD+   Y  + T         S +++     D  D L    +    WD+     
Sbjct: 43  AATMHPQDN--LYWTMNTNAYKFGFSGSDNASFYGSYDMNDHLSRMSIGRTNWDYHPMVN 102

Query: 88  LADDEET----------NDENEDMDE---DEGD--------FEDFDLDELSYEELIALGE 147
           +ADD E            DE+ + +E   +E D         +D D D ++YEEL+ LGE
Sbjct: 103 VADDPENTVARSVQIGDTDEHSEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGE 162

Query: 148 FIGEEKRGLPMNEIPSCLHSSKFR---TIENRSGIDRCVICQVEYEDGEELASLPCEHPY 201
            +G E RGL   E+   L + K++       +   +RCVICQ++Y+ GE   +LPC+H Y
Sbjct: 163 AVGTESRGL-SQELIETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVY 222

[Close]

BLAST of Lcy09g011440.1 vs. TAIR 10
Match: AT2G15530.1 (RING/U-box superfamily protein )

HSP 1 Score: 91.7 bits (226), Expect = 7.7e-19
Identity = 42/98 (42.86%), Postives = 59/98 (60.20%), Query Frame = 0


Query: 107 DLDELSYEELIALGEFIGEEKRGLPMNEIPSCLH-----SSKFRTIENRSGIDRCVICQV 166
           D+D +SYEEL+ALGE IG+   GL    I   +      SS   ++E    I+ C ICQ 
Sbjct: 602 DVDNMSYEELLALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIEPCCICQE 661

Query: 167 EYEDGEELASLPCEHPYHSECISKWLQVKRVCPICGTE 200
           EY +G+ L +L C H +H +CI +W+ +K +CPIC TE
Sbjct: 662 EYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

The following BLAST results are available for this feature:

BLAST of Lcy09g011440.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LT17	1.5e-22	32.05	E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR P...	[more]
Q8L649	1.7e-18	33.66	E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE...	[more]
Q9ZQF9	1.1e-17	42.86	E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV...	[more]
Q7XTV7	1.6e-16	43.48	Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399...	[more]
Q9FMM4	3.0e-15	33.57	Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]

BLAST of Lcy09g011440.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CJH0	4.3e-94	87.32	E3 ubiquitin ligase BIG BROTHER-related OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1GTM7	6.5e-90	85.38	E3 ubiquitin ligase BIG BROTHER-related OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1JR32	1.1e-89	85.38	E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A0A0KFS4	1.1e-86	79.72	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G382920 P...	[more]
A0A6J1K3Q9	2.2e-85	82.94	E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]

BLAST of Lcy09g011440.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141536.1	9.0e-94	87.32	E3 ubiquitin ligase BIG BROTHER-related [Momordica charantia]	[more]
XP_038886321.1	1.0e-89	84.36	E3 ubiquitin ligase BIG BROTHER-related-like [Benincasa hispida] >XP_038886322.1...	[more]
XP_022954880.1	1.3e-89	85.38	E3 ubiquitin ligase BIG BROTHER-related [Cucurbita moschata]	[more]
XP_022990800.1	2.3e-89	85.38	E3 ubiquitin ligase BIG BROTHER-related-like [Cucurbita maxima]	[more]
KAG7032814.1	5.1e-89	84.51	E3 ubiquitin ligase BIG BROTHER-related, partial [Cucurbita argyrosperma subsp. ...	[more]

BLAST of Lcy09g011440.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G47180.1	5.9e-35	41.67	RING/U-box superfamily protein	[more]
AT3G19910.1	1.0e-23	32.05	RING/U-box superfamily protein	[more]
AT3G63530.1	1.2e-19	33.66	RING/U-box superfamily protein	[more]
AT3G63530.2	1.2e-19	33.66	RING/U-box superfamily protein	[more]
AT2G15530.1	7.7e-19	42.86	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 156..196 e-value: 1.5E-5 score: 34.5
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 155..196 e-value: 1.8E-11 score: 44.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 156..196 score: 11.486073
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 133..206 e-value: 1.3E-17 score: 64.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 42..65
None	No IPR available	PANTHER	PTHR46400:SF8	E3 UBIQUITIN LIGASE BIG BROTHER-RELATED-LIKE	coord: 1..205
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 155..203
IPR033276	E3 ubiquitin-protein ligase BIG BROTHER	PANTHER	PTHR46400	RING/U-BOX SUPERFAMILY PROTEIN	coord: 1..205

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lcy09g011440	Lcy09g011440	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy09g011440.1.exon1	Lcy09g011440.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy09g011440.1.cds1	Lcy09g011440.1.cds1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lcy09g011440.1	Lcy09g011440.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046621	negative regulation of organ growth
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
cellular_component	GO:0019774	proteasome core complex, beta-subunit complex
molecular_function	GO:0004842	ubiquitin-protein transferase activity
molecular_function	GO:0004298	threonine-type endopeptidase activity
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding