Lcy09g001600.1 (mRNA) Sponge gourd (P93075) v1

Overview


Name	Lcy09g001600.1
Type	mRNA
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Cytoplasmic tRNA 2-thiolation protein 1
Location	Chr09: 1344762 .. 1345171 (-)
Sequence length	410
RNA-Seq Expression	Lcy09g001600.1
Synteny	Lcy09g001600.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACAACAACGAGAAGAAAGGAGGAGGAGGCTTCTTTCAAATGCCACTTCACTATCCAAGATACTCGAAGAAGGACTACGAGAACATGCCTGAATGGAAGCTCGACCGCCTTCTCGTCGAGTACGGCCTCCCGACTCGTGGCGACTTGCCCTACAAACGGCAGTTCGCAATGGGCGCTTTCCTTTGGCCTGATTTCCACCGTAGCTTTAAAGATCCCTCTCTTCTCAACAAAAAACTTGTGTAGTTAAAGTTGAAGCCCTTGCAGCCTTTTGGCTCTCTCTCTCTTTGTATTTGGTCTATAATGTGTAAGAGTTCCATAGGGCAGTGTTGGTGGGAGTCTCTCAAAAACACCATCCATTTCCCTAGCTTTTAATTCTTCACTTTCATGTCCATCATGCAATGAAATAT

mRNA sequence

ATGGACAACAACGAGAAGAAAGGAGGAGGAGGCTTCTTTCAAATGCCACTTCACTATCCAAGATACTCGAAGAAGGACTACGAGAACATGCCTGAATGGAAGCTCGACCGCCTTCTCGTCGAGTACGGCCTCCCGACTCGTGGCGACTTGCCCTACAAACGGCAGTTCGCAATGGGCGCTTTCCTTTGGCCTGATTTCCACCGTAGCTTTAAAGATCCCTCTCTTCTCAACAAAAAACTTGTGTAGTTAAAGTTGAAGCCCTTGCAGCCTTTTGGCTCTCTCTCTCTTTGTATTTGGTCTATAATGTGTAAGAGTTCCATAGGGCAGTGTTGGTGGGAGTCTCTCAAAAACACCATCCATTTCCCTAGCTTTTAATTCTTCACTTTCATGTCCATCATGCAATGAAATAT

Coding sequence (CDS)

ATGGACAACAACGAGAAGAAAGGAGGAGGAGGCTTCTTTCAAATGCCACTTCACTATCCAAGATACTCGAAGAAGGACTACGAGAACATGCCTGAATGGAAGCTCGACCGCCTTCTCGTCGAGTACGGCCTCCCGACTCGTGGCGACTTGCCCTACAAACGGCAGTTCGCAATGGGCGCTTTCCTTTGGCCTGATTTCCACCGTAGCTTTAAAGATCCCTCTCTTCTCAACAAAAAACTTGTGTAG

Protein sequence

MDNNEKKGGGGFFQMPLHYPRYSKKDYENMPEWKLDRLLVEYGLPTRGDLPYKRQFAMGAFLWPDFHRSFKDPSLLNKKLV

Homology

[Close]

BLAST of Lcy09g001600.1 vs. ExPASy TrEMBL
Match: A0A2P5ALC9 (Uncharacterized protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_320780 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 3.1e-24
Identity = 53/76 (69.74%), Postives = 63/76 (82.89%), Query Frame = 0


Query: 5  EKKGGGGFFQMPLHYPRYSKKDYENMPEWKLDRLLVEYGLPTRGDLPYKRQFAMGAFLWP 64
          +   G  +FQMPLHYPRY+KKDY++MPEWKLDRLL EYGL T GDL YKR FA+GAFLWP
Sbjct: 25 DSNNGVSYFQMPLHYPRYTKKDYQDMPEWKLDRLLAEYGLSTHGDLAYKRDFAIGAFLWP 84

Query: 65 DFHRSFKDPSLLNKKL 81
          D+ R+F D SLL+KK+
Sbjct: 85 DY-RTFTDASLLDKKV 99

[Close]

BLAST of Lcy09g001600.1 vs. ExPASy TrEMBL
Match: A0A2P5CX72 (Cytoplasmic tRNA 2-thiolation protein OS=Trema orientale OX=63057 GN=TorRG33x02_270200 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 5.3e-24
Identity = 54/87 (62.07%), Postives = 64/87 (73.56%), Query Frame = 0


Query: 5   EKKGGGGFFQMPLHYPRYSKKDYENMPEWKLDRLLVEYGLPTRGDLPYKRQFAMGAFLWP 64
           +   G  +FQMPLHYPRY+KKDY++MPEWKLDRLL EYGL T GDL YKR FA+GAFLWP
Sbjct: 25  DSNNGVSYFQMPLHYPRYTKKDYQDMPEWKLDRLLAEYGLSTHGDLAYKRDFAIGAFLWP 84

Query: 65  DFH-----------RSFKDPSLLNKKL 81
           D+H           R+F D SLL+KK+
Sbjct: 85  DYHQDRKPSSSLSYRTFTDASLLDKKV 111

[Close]

BLAST of Lcy09g001600.1 vs. ExPASy TrEMBL
Match: A0A061FTQ6 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Theobroma cacao OX=3641 GN=TCM_042740 PE=4 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 9.1e-24
Identity = 50/64 (78.12%), Postives = 57/64 (89.06%), Query Frame = 0


Query: 4  NEKKGGGGFFQMPLHYPRYSKKDYENMPEWKLDRLLVEYGLPTRGDLPYKRQFAMGAFLW 63
          +EK+ G  FFQMPLHYPRY++KDY++MPEWKLDRLL EYGL  +GDL YKRQFAMGAFLW
Sbjct: 3  SEKQNGVSFFQMPLHYPRYTQKDYQDMPEWKLDRLLAEYGLSNKGDLAYKRQFAMGAFLW 62

Query: 64 PDFH 68
          PDFH
Sbjct: 63 PDFH 66

[Close]

BLAST of Lcy09g001600.1 vs. ExPASy TrEMBL
Match: W9SNS4 (Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_010408 PE=4 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 9.1e-24
Identity = 52/85 (61.18%), Postives = 65/85 (76.47%), Query Frame = 0


Query: 5  EKKGGGGFFQMPLHYPRYSKKDYENMPEWKLDRLLVEYGLPTRGDLPYKRQFAMGAFLWP 64
          + + G  +FQMPLHYPRY+K+DY++MPEWKLD+LL +YGLPT GDL YKR FA+GAFLWP
Sbjct: 7  KNESGVSYFQMPLHYPRYTKQDYQDMPEWKLDQLLAQYGLPTHGDLAYKRDFAIGAFLWP 66

Query: 65 DFH---------RSFKDPSLLNKKL 81
          DFH         R+  D SLL+KK+
Sbjct: 67 DFHQGSKPPPPYRTINDASLLDKKI 91

[Close]

BLAST of Lcy09g001600.1 vs. ExPASy TrEMBL
Match: A0A1R3I234 (Uncharacterized protein OS=Corchorus olitorius OX=93759 GN=COLO4_25500 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.6e-23
Identity = 49/67 (73.13%), Postives = 59/67 (88.06%), Query Frame = 0


Query: 1  MDNNEKKGGGGFFQMPLHYPRYSKKDYENMPEWKLDRLLVEYGLPTRGDLPYKRQFAMGA 60
          M++++K+ G  FFQMPLHYPRY+KKDY+ M EWK+DRLL +YGL T+GDL YKRQFAMGA
Sbjct: 1  MESSKKQNGVSFFQMPLHYPRYTKKDYQEMAEWKVDRLLADYGLSTKGDLDYKRQFAMGA 60

Query: 61 FLWPDFH 68
          FLWPDFH
Sbjct: 61 FLWPDFH 67

[Close]

BLAST of Lcy09g001600.1 vs. NCBI nr
Match: KAG6578420.1 (hypothetical protein SDJN03_22868, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015984.1 hypothetical protein SDJN02_21088, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 146.7 bits (369), Expect = 8.4e-32
Identity = 68/83 (81.93%), Postives = 74/83 (89.16%), Query Frame = 0


Query: 1  MDNNEKK-GGGGFFQMPLHYPRYSKKDYENMPEWKLDRLLVEYGLPTRGDLPYKRQFAMG 60
          M+NNEKK GG  FFQMPLHYPRYSK DYENMPEWKLDRLL+EYGLPT G+LPYKRQFAMG
Sbjct: 1  MENNEKKQGGDNFFQMPLHYPRYSKMDYENMPEWKLDRLLLEYGLPTHGNLPYKRQFAMG 60

Query: 61 AFLWPDFHRSFK-DPSLLNKKLV 82
          AFLW D+H +FK DPSLLN KL+
Sbjct: 61 AFLWHDYHPTFKLDPSLLNNKLM 83

[Close]

BLAST of Lcy09g001600.1 vs. NCBI nr
Match: PON37357.1 (hypothetical protein PanWU01x14_320780 [Parasponia andersonii])

HSP 1 Score: 120.6 bits (301), Expect = 6.5e-24
Identity = 53/76 (69.74%), Postives = 63/76 (82.89%), Query Frame = 0


Query: 5  EKKGGGGFFQMPLHYPRYSKKDYENMPEWKLDRLLVEYGLPTRGDLPYKRQFAMGAFLWP 64
          +   G  +FQMPLHYPRY+KKDY++MPEWKLDRLL EYGL T GDL YKR FA+GAFLWP
Sbjct: 25 DSNNGVSYFQMPLHYPRYTKKDYQDMPEWKLDRLLAEYGLSTHGDLAYKRDFAIGAFLWP 84

Query: 65 DFHRSFKDPSLLNKKL 81
          D+ R+F D SLL+KK+
Sbjct: 85 DY-RTFTDASLLDKKV 99

[Close]

BLAST of Lcy09g001600.1 vs. NCBI nr
Match: PON65649.1 (cytoplasmic tRNA 2-thiolation protein [Trema orientale])

HSP 1 Score: 119.8 bits (299), Expect = 1.1e-23
Identity = 54/87 (62.07%), Postives = 64/87 (73.56%), Query Frame = 0


Query: 5   EKKGGGGFFQMPLHYPRYSKKDYENMPEWKLDRLLVEYGLPTRGDLPYKRQFAMGAFLWP 64
           +   G  +FQMPLHYPRY+KKDY++MPEWKLDRLL EYGL T GDL YKR FA+GAFLWP
Sbjct: 25  DSNNGVSYFQMPLHYPRYTKKDYQDMPEWKLDRLLAEYGLSTHGDLAYKRDFAIGAFLWP 84

Query: 65  DFH-----------RSFKDPSLLNKKL 81
           D+H           R+F D SLL+KK+
Sbjct: 85  DYHQDRKPSSSLSYRTFTDASLLDKKV 111

[Close]

BLAST of Lcy09g001600.1 vs. NCBI nr
Match: XP_010108400.1 (uncharacterized protein LOC21393700 [Morus notabilis] >EXC19391.1 hypothetical protein L484_010408 [Morus notabilis])

HSP 1 Score: 119.0 bits (297), Expect = 1.9e-23
Identity = 52/85 (61.18%), Postives = 65/85 (76.47%), Query Frame = 0


Query: 5  EKKGGGGFFQMPLHYPRYSKKDYENMPEWKLDRLLVEYGLPTRGDLPYKRQFAMGAFLWP 64
          + + G  +FQMPLHYPRY+K+DY++MPEWKLD+LL +YGLPT GDL YKR FA+GAFLWP
Sbjct: 7  KNESGVSYFQMPLHYPRYTKQDYQDMPEWKLDQLLAQYGLPTHGDLAYKRDFAIGAFLWP 66

Query: 65 DFH---------RSFKDPSLLNKKL 81
          DFH         R+  D SLL+KK+
Sbjct: 67 DFHQGSKPPPPYRTINDASLLDKKI 91

[Close]

BLAST of Lcy09g001600.1 vs. NCBI nr
Match: EOY18099.1 (Cytoplasmic tRNA 2-thiolation protein 1 [Theobroma cacao])

HSP 1 Score: 119.0 bits (297), Expect = 1.9e-23
Identity = 50/64 (78.12%), Postives = 57/64 (89.06%), Query Frame = 0


Query: 4  NEKKGGGGFFQMPLHYPRYSKKDYENMPEWKLDRLLVEYGLPTRGDLPYKRQFAMGAFLW 63
          +EK+ G  FFQMPLHYPRY++KDY++MPEWKLDRLL EYGL  +GDL YKRQFAMGAFLW
Sbjct: 3  SEKQNGVSFFQMPLHYPRYTQKDYQDMPEWKLDRLLAEYGLSNKGDLAYKRQFAMGAFLW 62

Query: 64 PDFH 68
          PDFH
Sbjct: 63 PDFH 66

[Close]

BLAST of Lcy09g001600.1 vs. TAIR 10
Match: AT3G55570.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G41761.1); Has 128 Blast hits to 128 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 128; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 102.1 bits (253), Expect = 2.2e-22
Identity = 46/78 (58.97%), Postives = 58/78 (74.36%), Query Frame = 0


Query: 4  NEKKGGGGFFQMPLHYPRYSKKDYENMPEWKLDRLLVEYGLPTRGDLPYKRQFAMGAFLW 63
          N K G G  F+MPLHYPRYSK+DY++MPEWKLDR+L +YGL T GDL +KR FA+GAFLW
Sbjct: 23 NGKNGEGSVFRMPLHYPRYSKEDYQDMPEWKLDRVLADYGLSTYGDLAHKRDFAIGAFLW 82

Query: 64 PDFHRSFKDPSLLNKKLV 82
               S ++P L  +K +
Sbjct: 83 ----ISTRNPKLYQEKSI 96

[Close]

BLAST of Lcy09g001600.1 vs. TAIR 10
Match: AT5G41761.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G55570.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 87.4 bits (215), Expect = 5.6e-18
Identity = 37/63 (58.73%), Postives = 49/63 (77.78%), Query Frame = 0


Query: 1  MDNNEKKGGGGFFQMPLHYPRYSKKDYENMPEWKLDRLLVEYGLPTRGDLPYKRQFAMGA 60
          +++++ +     FQ+PLHYP+Y+K DYE MPEW+LDRLL EYGLP  GD   KR+FA+GA
Sbjct: 33 INHDKPQNQSSSFQIPLHYPKYTKSDYEKMPEWQLDRLLREYGLPVIGDSYEKRKFAIGA 92

Query: 61 FLW 64
          FLW
Sbjct: 93 FLW 95

[Close]

BLAST of Lcy09g001600.1 vs. TAIR 10
Match: AT3G09950.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G41761.1); Has 128 Blast hits to 128 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 128; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 72.0 bits (175), Expect = 2.5e-13
Identity = 35/64 (54.69%), Postives = 41/64 (64.06%), Query Frame = 0


Query: 3  NNEKKGGGGFFQMPLHYPRYSKKDYENMPEWKLDRLLVEYGLPTRGD--LPYKRQFAMGA 62
          N   K     F+MPLHYPRY+K+DYE M EW+LD LL EYGL    D  L  KR FA+  
Sbjct: 26 NGAVKAPSSGFKMPLHYPRYTKEDYEEMEEWRLDLLLSEYGLLAFHDNTLHEKRAFAIDT 85

Query: 63 FLWP 65
          F+WP
Sbjct: 86 FIWP 89

[Close]

BLAST of Lcy09g001600.1 vs. TAIR 10
Match: AT5G55620.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G09950.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 71.2 bits (173), Expect = 4.2e-13
Identity = 31/53 (58.49%), Postives = 38/53 (71.70%), Query Frame = 0


Query: 13 FQMPLHYPRYSKKDYENMPEWKLDRLLVEYGLPTRGDLPYKRQFAMGAFLWPD 66
          FQ+PLHYP+YSK DYE M + +LD LL +YG    G L  KR FA+ +FLWPD
Sbjct: 47 FQVPLHYPKYSKSDYEVMDDLRLDLLLKQYGFSFEGSLEDKRVFAIESFLWPD 99

[Close]

BLAST of Lcy09g001600.1 vs. TAIR 10
Match: AT3G11405.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G55570.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 67.8 bits (164), Expect = 4.6e-12
Identity = 32/52 (61.54%), Postives = 40/52 (76.92%), Query Frame = 0


Query: 13  FQMPLHYPRYSKKDYENMPEWKLDRLLVEYGLPTR-GDLPYKRQFAMGAFLW 64
           FQMPL YP Y+K+ Y+ M E +LDRLL  YGLPT  G+L  K++FA+GAFLW
Sbjct: 57  FQMPLQYPNYAKEQYDIMSEEELDRLLKLYGLPTDIGNLSCKKEFAVGAFLW 108

The following BLAST results are available for this feature:

BLAST of Lcy09g001600.1 vs.


Match Name	E-value	Identity	Description

BLAST of Lcy09g001600.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2P5ALC9	3.1e-24	69.74	Uncharacterized protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_320780 PE...	[more]
A0A2P5CX72	5.3e-24	62.07	Cytoplasmic tRNA 2-thiolation protein OS=Trema orientale OX=63057 GN=TorRG33x02_...	[more]
A0A061FTQ6	9.1e-24	78.13	Cytoplasmic tRNA 2-thiolation protein 1 OS=Theobroma cacao OX=3641 GN=TCM_042740...	[more]
W9SNS4	9.1e-24	61.18	Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_010408 PE=4 SV=1	[more]
A0A1R3I234	1.6e-23	73.13	Uncharacterized protein OS=Corchorus olitorius OX=93759 GN=COLO4_25500 PE=4 SV=1	[more]

BLAST of Lcy09g001600.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6578420.1	8.4e-32	81.93	hypothetical protein SDJN03_22868, partial [Cucurbita argyrosperma subsp. sorori...	[more]
PON37357.1	6.5e-24	69.74	hypothetical protein PanWU01x14_320780 [Parasponia andersonii]	[more]
PON65649.1	1.1e-23	62.07	cytoplasmic tRNA 2-thiolation protein [Trema orientale]	[more]
XP_010108400.1	1.9e-23	61.18	uncharacterized protein LOC21393700 [Morus notabilis] >EXC19391.1 hypothetical p...	[more]
EOY18099.1	1.9e-23	78.13	Cytoplasmic tRNA 2-thiolation protein 1 [Theobroma cacao]	[more]

BLAST of Lcy09g001600.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G55570.1	2.2e-22	58.97	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT5G41761.1	5.6e-18	58.73	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT3G09950.1	2.5e-13	54.69	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT5G55620.1	4.2e-13	58.49	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT3G11405.1	4.6e-12	61.54	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33513:SF4	OS01G0221400 PROTEIN	coord: 6..65
None	No IPR available	PANTHER	PTHR33513	OS06G0523300 PROTEIN	coord: 6..65

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lcy09g001600	Lcy09g001600	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy09g001600.1.exon1	Lcy09g001600.1.exon1	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy09g001600.1.utr3p1	Lcy09g001600.1.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy09g001600.1.cds1	Lcy09g001600.1.cds1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lcy09g001600.1	Lcy09g001600.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016779	nucleotidyltransferase activity