Lcy03g017600.1 (mRNA) Sponge gourd (P93075) v1

Overview


Name	Lcy03g017600.1
Type	mRNA
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Phospholipase A2-alpha
Location	Chr03: 51397092 .. 51400037 (+)
Sequence length	462
RNA-Seq Expression	Lcy03g017600.1
Synteny	Lcy03g017600.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTCGATTTCAGTTCTTGAAGTTGTTGCTTCTTCTTCTCTGCCTCGGCCTTGCCTCTGCGCCCGCTCACGCCCTCAACATCGGCGTTCAGGCCCTCGATGCCTCAATCACTATGGTTCTCTTCTTCTTCTTCACTTTCTGCTTCTTGATTTTGTCTTTGGTTTCATTAATTTCATCTGTTTGGTTTGTTTTGCAGAGCAAAGAATGCAGTAGAAAGTGCGAGTCGGAGTTCTGTTCAGGTTCTTCTCATTAATTATCTCTCATTCTCTAATTTTTGTGGGTTTTCTTTTTTTGTGTTGATTTCGTGTTGTTGTTCGTTCTTTGCAGTGCCTCCACTTTTGAGATATGGCAAATATTGTGGGCTCATGTATAGCGGCTGCCCTGGCGAACGCCCCTGCGATGGCTTGGATGCTTGCTGTATGAAGCACGATGCCTGTGTTGTTGCCAAGAACAGTCAGTTTTCTTTTCTCTCTCTTGTTTCTCTTTCCTTTTTGGAACCCAACAATACTTGTCTACACACGGGTCAATTGAGAAATTAGCTGAAAAGGTTGGAGTAACACTGTTGGATGTTCTGAGAAAGAACTTGATTAATGTATGTCTTGAATAGGAGAAATTATTCGATAATACTGCTGCAATGAGACTAACAGTTTTGCACTGTTGGATATAAAAGAAAAATTATAGAAGTGTTTCGCTTTTATATCCAACGGTTAGTAGGGACAAGTAGATTTGCAAATTCACATTCGACGGTTACTGTAAGACAACCAACATTGTGGTTGAACCTCATTGATAACAGTTTTTTTTTTAAGTTCAACAATACTTGGGGGTGAGGGATTTGAACTTCTTATCTCTTGGTCGAGAATACATGTCAATTACCACTAAGCTATGCTCGTTTTGGCCTCATTGATAACAGTTATTAGGAGTTGATAGATTTTGAATCTACATCCATCTGTTGTTAGAGGTAGTCAAATTGTGGCGGTAGGGTTGAATAATTTTTTTGGGTTAATGGGATTTTTTTTTAGAAAAAAAGTTTTGAGTAGATAAGTGTGAATCTTGTTGACATATTTAACTGCTAAAAATGAACCAAGTCGGAACGTAGTAAGAGCTATTGTTGGGTAGACAAGTATTGTTCCTTTCAAACATGCTTTTTACACACGAATTATGTACCACTTTGTATCTTTCTTTGGCTTTGTTTATGTTATTAAAGTTGTTTTTTTTTTTTTTTTTTGAAGAAAGTTATTAAAGTTGTTGGTATCACTTTGGCGTGACATGTTTATTGAATAAATTTTGGTGTGATTAGTGTGATGTGATAGGACAAGTCACGACAACGCTTTGTTAGTGTGATGTGAGATAGAGTTGGATAGGTAATCCCTGTCATTATAGGAATTTTGTAGGAGTATGATTCAAAAATTAGAGATGTATTGAACTTTTCTTTTGGAAATGAAGGGATGTGGGTGATAGGATATTGAAGTTTAGGGAATAAATTTACAATTTAATCTTGCTTTACACTTAATAAGGTGTTGTTTTGTCCAATTAATATTTTTGAAGTTAGGTTGTTTGATGAGAAGGCTAAATTTTGTCTCTTTGTTTGAGTGAACCAATAAACCTCACATGCAAGATGCCTAAGAAGGTCTATTTATAATTATAGCTTACTTTATGATTAATTAAATGAAGTCTATTTATTAAAAGAAAACAGATAATTCCAGCCTATAATTTTCATTTTCAATTTCTTAATGTTCAATAGAATTAAGTGGAGCCTTATTGTTCTCCTTAAAATTGGAGTTTCCTACCGAACTCAATTTTCAAAATGTCTAATTTCATTTTTTTTTAAACTCTCAAATTTAATTCATTTTAACTTTCAAGAGTTATATTATAAACTATAATTTTTTTTAAAAAAATATATTTCTTTTGATTATTGTCTTTATTTATCACTTTTAACAAATAGGTACTATAGTTTCAAATATGTACATTAATGCGAGAACATGTATTTGAGTCATATGTGTTGGTTGAAATAAGTATTAGGTGAGTGAATATATGTGTGTGTTTGATAATAAGGTGAGCAAATATTAGAGAGAAAAGTAATAATGAGAACTACAATAGATCATTTTTTAAGCTTAAACACTAAAATAGACGATATGAAAGTTTAAGAATCAAGATGGGTATTTTACTAAAATAGATGTCAACATAAGAAAAACTTGGTTATAATCCATGAGCAAAATAACCAAGAGTAAACTAAAACATATTTTGTTCTCAAAAACAATATATTTTGGTAGTCTATAATCCATCTATACAATATTTTGTTTTCAACGACAAGCTCTTTCAAGTTACTTATAAAAGTAAAGCATGAAAAACTACCTGGATTGATCAGGTGGCCAAAAGTCAAAGTGGTCGAGCATAGTTTAGTAATAATTGATATGTATTCCTCTTGAAGTCGGAGATTCAAATTCTCATGCTCCACATTTGTTGTCCTAAAAAAAACTAGTGGTTAGGAAGAGCCATGTAAATATGAAGGGCTTTGAGAGAATGAGTTCAAGAGTCACGGTGACTATCTACATAGAATTTAATATCTTAAGAATTACTTTGACAACCAAATCATGAAGTAGGGTCAGGTGGTCCTCCCAAGGTCCATGAAACTAATTCCTTCTAAAACATAAAGCATTCGTAAAAACAAAGGACTACAAAAGGAAGTTAAAAGAAGAATCGTGAATGAAACACATGTTACGGGTAAAACTTTGCACATATATAAATGGTGGGTTGTGTTTGTTTTGTAGATGACTATCTAAGCCAAGAGTGCAGCCAAAGCTTCTTGAATTGCATGGAGAAGTTCAAGAAATCAAAATCAACTCCTTTCAAAGGAAACAAATGCCAAATTGATGAAGTCATTGATGTCATCTCCTTTGTCATGGAAGCTGCCCTTATTGCAGGCAGAGTTTTTCACAAGCCCTAG

mRNA sequence

ATGACTCGATTTCAGTTCTTGAAGTTGTTGCTTCTTCTTCTCTGCCTCGGCCTTGCCTCTGCGCCCGCTCACGCCCTCAACATCGGCGTTCAGGCCCTCGATGCCTCAATCACTATGAGCAAAGAATGCAGTAGAAAGTGCGAGTCGGAGTTCTGTTCAGTGCCTCCACTTTTGAGATATGGCAAATATTGTGGGCTCATGTATAGCGGCTGCCCTGGCGAACGCCCCTGCGATGGCTTGGATGCTTGCTGTATGAAGCACGATGCCTGTGTTGTTGCCAAGAACAATGACTATCTAAGCCAAGAGTGCAGCCAAAGCTTCTTGAATTGCATGGAGAAGTTCAAGAAATCAAAATCAACTCCTTTCAAAGGAAACAAATGCCAAATTGATGAAGTCATTGATGTCATCTCCTTTGTCATGGAAGCTGCCCTTATTGCAGGCAGAGTTTTTCACAAGCCCTAG

Coding sequence (CDS)

ATGACTCGATTTCAGTTCTTGAAGTTGTTGCTTCTTCTTCTCTGCCTCGGCCTTGCCTCTGCGCCCGCTCACGCCCTCAACATCGGCGTTCAGGCCCTCGATGCCTCAATCACTATGAGCAAAGAATGCAGTAGAAAGTGCGAGTCGGAGTTCTGTTCAGTGCCTCCACTTTTGAGATATGGCAAATATTGTGGGCTCATGTATAGCGGCTGCCCTGGCGAACGCCCCTGCGATGGCTTGGATGCTTGCTGTATGAAGCACGATGCCTGTGTTGTTGCCAAGAACAATGACTATCTAAGCCAAGAGTGCAGCCAAAGCTTCTTGAATTGCATGGAGAAGTTCAAGAAATCAAAATCAACTCCTTTCAAAGGAAACAAATGCCAAATTGATGAAGTCATTGATGTCATCTCCTTTGTCATGGAAGCTGCCCTTATTGCAGGCAGAGTTTTTCACAAGCCCTAG

Protein sequence

MTRFQFLKLLLLLLCLGLASAPAHALNIGVQALDASITMSKECSRKCESEFCSVPPLLRYGKYCGLMYSGCPGERPCDGLDACCMKHDACVVAKNNDYLSQECSQSFLNCMEKFKKSKSTPFKGNKCQIDEVIDVISFVMEAALIAGRVFHKP

Homology

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BLAST of Lcy03g017600.1 vs. ExPASy Swiss-Prot
Match: Q8S8N6 (Phospholipase A2-alpha OS=Arabidopsis thaliana OX=3702 GN=PLA2-ALPHA PE=1 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 2.3e-57
Identity = 103/144 (71.53%), Postives = 116/144 (80.56%), Query Frame = 0


Query: 10  LLLLLCLGLASAPAHALNIGVQALDASITMSKECSRKCESEFCSVPPLLRYGKYCGLMYS 69
           ++L   L   S    ALN+GVQ +  SI+++KECSRKCESEFCSVPP LRYGKYCGL+YS
Sbjct: 5   IILFSFLLFFSVSVSALNVGVQLIHPSISLTKECSRKCESEFCSVPPFLRYGKYCGLLYS 64

Query: 70  GCPGERPCDGLDACCMKHDACVVAKNNDYLSQECSQSFLNCMEKFKKSKSTPFKGNKCQI 129
           GCPGERPCDGLD+CCMKHDACV +KNNDYLSQECSQ F+NCM  F + K   FKGNKC  
Sbjct: 65  GCPGERPCDGLDSCCMKHDACVQSKNNDYLSQECSQKFINCMNNFSQKKQPTFKGNKCDA 124

Query: 130 DEVIDVISFVMEAALIAGRVFHKP 154
           DEVIDVIS VMEAALIAG+V  KP
Sbjct: 125 DEVIDVISIVMEAALIAGKVLKKP 148

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BLAST of Lcy03g017600.1 vs. ExPASy Swiss-Prot
Match: Q10E50 (Phospholipase A2 homolog 3 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-III PE=1 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.3e-44
Identity = 84/141 (59.57%), Postives = 106/141 (75.18%), Query Frame = 0


Query: 13  LLCLGLASAPAHALNIGVQALDASITMSKECSRKCESEFCSVPPLLRYGKYCGLMYSGCP 72
           LL   + + PA ALNIG+Q+     + +  CSR CES+ C+ PPLLRYGKYCG++YSGCP
Sbjct: 25  LLLFAVVAPPAAALNIGLQSAGDGASKAGLCSRTCESDHCTTPPLLRYGKYCGILYSGCP 84

Query: 73  GERPCDGLDACCMKHDACVVAKNNDYLSQECSQSFLNCMEKFKKSKSTPFKGNKCQIDEV 132
           GE+PCD LDACCM HD CV AK NDYLS  C++  L C+ + ++  ST F+GNKC IDEV
Sbjct: 85  GEQPCDELDACCMHHDNCVQAK-NDYLSTACNEELLECLARLREGSST-FQGNKCMIDEV 144

Query: 133 IDVISFVMEAALIAGRVFHKP 154
           IDVIS V+EAA++AGR+ HKP
Sbjct: 145 IDVISLVIEAAVVAGRLLHKP 163

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BLAST of Lcy03g017600.1 vs. ExPASy Swiss-Prot
Match: Q9XG81 (Probable phospholipase A2 homolog 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-II PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 5.5e-35
Identity = 76/152 (50.00%), Postives = 100/152 (65.79%), Query Frame = 0


Query: 6   FLKL-----LLLLLCLGLASAPAHALNIGVQALDASITMSKECSRKCESEFCSVPPLLRY 65
           FLKL     +LLLL L  AS     LNIG   L ++    + CSR CES+FC++ PLLRY
Sbjct: 4   FLKLAPRCSVLLLLLLVTAS---RGLNIG-DLLGSTPAKDQGCSRTCESQFCTIAPLLRY 63

Query: 66  GKYCGLMYSGCPGERPCDGLDACCMKHDACVVAKNNDYLSQECSQSFLNCMEKFKKSKST 125
           GKYCG++YSGCPGERPCD LDACCM HD CV   N+DYL+  C+++ L+C+++       
Sbjct: 64  GKYCGILYSGCPGERPCDALDACCMVHDHCVDTHNDDYLNTMCNENLLSCIDRV---SGA 123

Query: 126 PFKGNKCQIDEVIDVISFVMEAALIAGRVFHK 153
            F GNKC + +   VI  V+E A+ AG++ HK
Sbjct: 124 TFPGNKCNVGQTASVIRGVIETAVFAGKILHK 148

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BLAST of Lcy03g017600.1 vs. ExPASy Swiss-Prot
Match: Q9XG80 (Probable phospholipase A2 homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-I PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.5e-16
Identity = 44/107 (41.12%), Postives = 61/107 (57.01%), Query Frame = 0


Query: 43  CSRKCESEFCSVPPLLRYGKYCGLMYSGCPGERPCDGLDACCMKHDACVVAKNNDYLSQE 102
           CSR C +  C     +RYGKYCG+ +SGC GE PCD LDACC  HD CV  K    +S +
Sbjct: 29  CSRSCAALNCDSVG-IRYGKYCGVGWSGCDGEEPCDDLDACCRDHDHCVDKKG--LMSVK 88

Query: 103 CSQSFLNCMEKFKKSKSTPFKGNKCQIDEVIDVISFVMEAALIAGRV 150
           C + F NCM K KK+    F   KC  +  +  ++  M+ A++  ++
Sbjct: 89  CHEKFKNCMRKVKKAGKIGF-SRKCPYEMAMATMTSGMDMAIMLSQL 131

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BLAST of Lcy03g017600.1 vs. ExPASy Swiss-Prot
Match: Q8GV50 (Phospholipase A2-delta OS=Arabidopsis thaliana OX=3702 GN=PLA2-DELTA PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.3e-16
Identity = 42/93 (45.16%), Postives = 58/93 (62.37%), Query Frame = 0


Query: 25  ALNIGVQALDASITMSKECSRKCESEFCSVPPLLRYGKYCGLMYSGCPGERPCDGLDACC 84
           AL + V  L A +   ++CS+ C ++ C+V   +RYGKYCG+ Y GCPGE PCD LD CC
Sbjct: 11  ALGLTVFLLLAVVHSQEKCSKTCIAQKCNVLG-IRYGKYCGIGYFGCPGEPPCDDLDDCC 70

Query: 85  MKHDACVVAKNNDYLSQECSQSFLNCMEKFKKS 118
           M HD CV  K   Y+  +C + F  C+ + K+S
Sbjct: 71  MTHDNCVDLKGMTYV--DCHKQFQRCVNELKQS 100

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BLAST of Lcy03g017600.1 vs. ExPASy TrEMBL
Match: A0A5D3DYM2 (Phospholipase A2-alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G001950 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.7e-71
Identity = 132/149 (88.59%), Postives = 141/149 (94.63%), Query Frame = 0


Query: 5   QFLKLLLLLLCLGLASAPAHALNIGVQALDASITMSKECSRKCESEFCSVPPLLRYGKYC 64
           QFL LLLLLL   +A  P+HALNIGVQALDASITMSK+CSRKCESEFCSVPPLLRYGKYC
Sbjct: 3   QFLLLLLLLLLPSIAFLPSHALNIGVQALDASITMSKDCSRKCESEFCSVPPLLRYGKYC 62

Query: 65  GLMYSGCPGERPCDGLDACCMKHDACVVAKNNDYLSQECSQSFLNCMEKFKKSKSTPFKG 124
           GL+YSGCPGERPCDGLDACCMKHDACVVAKN+DYLSQECSQSFLNCME FK+S+S PFKG
Sbjct: 63  GLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSRPFKG 122

Query: 125 NKCQIDEVIDVISFVMEAALIAGRVFHKP 154
           NKCQ+DEVIDVISFVMEAALIAG+VFHKP
Sbjct: 123 NKCQVDEVIDVISFVMEAALIAGKVFHKP 151

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BLAST of Lcy03g017600.1 vs. ExPASy TrEMBL
Match: A0A1S3BWG7 (phospholipase A2-alpha OS=Cucumis melo OX=3656 GN=LOC103494216 PE=4 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 2.4e-70
Identity = 133/156 (85.26%), Postives = 143/156 (91.67%), Query Frame = 0


Query: 1   MTRFQFLKLLLLLLCL---GLASAPAHALNIGVQALDASITMSKECSRKCESEFCSVPPL 60
           M +F  L LLLLLL L    +A  P+HALNIGVQALDASITMSK+CSRKCESEFCSVPPL
Sbjct: 1   MPQFLLLLLLLLLLLLLLPSIAFLPSHALNIGVQALDASITMSKDCSRKCESEFCSVPPL 60

Query: 61  LRYGKYCGLMYSGCPGERPCDGLDACCMKHDACVVAKNNDYLSQECSQSFLNCMEKFKKS 120
           LRYGKYCGL+YSGCPGERPCDGLDACCMKHDACVVAKN+DYLSQECSQSFLNCME FK+S
Sbjct: 61  LRYGKYCGLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRS 120

Query: 121 KSTPFKGNKCQIDEVIDVISFVMEAALIAGRVFHKP 154
           +S PFKGNKCQ+DEVIDVISFVMEAALIAG+VFHKP
Sbjct: 121 RSRPFKGNKCQVDEVIDVISFVMEAALIAGKVFHKP 156

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BLAST of Lcy03g017600.1 vs. ExPASy TrEMBL
Match: A0A6J1F1D2 (phospholipase A2-alpha OS=Cucurbita moschata OX=3662 GN=LOC111438456 PE=4 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 6.0e-69
Identity = 122/142 (85.92%), Postives = 134/142 (94.37%), Query Frame = 0


Query: 12  LLLCLGLASAPAHALNIGVQALDASITMSKECSRKCESEFCSVPPLLRYGKYCGLMYSGC 71
           LLLCL + SAP HALN+GVQALDASIT+SK+CSRKCESEFCSVPPLLRYGKYCGL+YSGC
Sbjct: 11  LLLCLAIVSAPTHALNVGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGC 70

Query: 72  PGERPCDGLDACCMKHDACVVAKNNDYLSQECSQSFLNCMEKFKKSKSTPFKGNKCQIDE 131
           PGERPCDGLDACCM HDACV AKNNDYLS+ECS+SFLNCMEKFK+SKS PFKGNKC+I E
Sbjct: 71  PGERPCDGLDACCMNHDACVAAKNNDYLSEECSKSFLNCMEKFKRSKSAPFKGNKCEISE 130

Query: 132 VIDVISFVMEAALIAGRVFHKP 154
           V++VISFVMEAAL+AGRV HKP
Sbjct: 131 VVEVISFVMEAALLAGRVLHKP 152

[Close]

BLAST of Lcy03g017600.1 vs. ExPASy TrEMBL
Match: A0A6J1IA72 (phospholipase A2-alpha OS=Cucurbita maxima OX=3661 GN=LOC111472609 PE=4 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 3.9e-68
Identity = 120/142 (84.51%), Postives = 133/142 (93.66%), Query Frame = 0


Query: 12  LLLCLGLASAPAHALNIGVQALDASITMSKECSRKCESEFCSVPPLLRYGKYCGLMYSGC 71
           LLLCL + SAP HALN+GVQALDASIT+SK+CSRKCESEFCSVPPLLRYGKYCGL+YSGC
Sbjct: 11  LLLCLAIVSAPTHALNVGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGC 70

Query: 72  PGERPCDGLDACCMKHDACVVAKNNDYLSQECSQSFLNCMEKFKKSKSTPFKGNKCQIDE 131
           PGERPCDGLDACCM HDACV AKNNDYLS+ECS+SFLNCMEKFK+SKS PFKGNKC+I E
Sbjct: 71  PGERPCDGLDACCMNHDACVAAKNNDYLSEECSKSFLNCMEKFKRSKSAPFKGNKCEISE 130

Query: 132 VIDVISFVMEAALIAGRVFHKP 154
           V++VISFVM+ AL+AGRV HKP
Sbjct: 131 VVEVISFVMDVALLAGRVLHKP 152

[Close]

BLAST of Lcy03g017600.1 vs. ExPASy TrEMBL
Match: A0A0A0LUV2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G433610 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 5.1e-68
Identity = 124/146 (84.93%), Postives = 136/146 (93.15%), Query Frame = 0


Query: 8   KLLLLLLCLGLASAPAHALNIGVQALDASITMSKECSRKCESEFCSVPPLLRYGKYCGLM 67
           + LLLLL   +   P+HALNIGVQALDAS+TMSK+CSRKCESEFCSVPPLLRYGKYCGL+
Sbjct: 3   QFLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLL 62

Query: 68  YSGCPGERPCDGLDACCMKHDACVVAKNNDYLSQECSQSFLNCMEKFKKSKSTPFKGNKC 127
           YSGCPGE PCDGLDACCMKHDACVVAKN+DYLSQECSQSFLNCME FK+S+S+PFKGNKC
Sbjct: 63  YSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKC 122

Query: 128 QIDEVIDVISFVMEAALIAGRVFHKP 154
           Q+DEVI+VIS VMEAALIAGRVFHKP
Sbjct: 123 QVDEVIEVISLVMEAALIAGRVFHKP 148

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BLAST of Lcy03g017600.1 vs. NCBI nr
Match: TYK28548.1 (phospholipase A2-alpha [Cucumis melo var. makuwa])

HSP 1 Score: 278.5 bits (711), Expect = 3.5e-71
Identity = 132/149 (88.59%), Postives = 141/149 (94.63%), Query Frame = 0


Query: 5   QFLKLLLLLLCLGLASAPAHALNIGVQALDASITMSKECSRKCESEFCSVPPLLRYGKYC 64
           QFL LLLLLL   +A  P+HALNIGVQALDASITMSK+CSRKCESEFCSVPPLLRYGKYC
Sbjct: 3   QFLLLLLLLLLPSIAFLPSHALNIGVQALDASITMSKDCSRKCESEFCSVPPLLRYGKYC 62

Query: 65  GLMYSGCPGERPCDGLDACCMKHDACVVAKNNDYLSQECSQSFLNCMEKFKKSKSTPFKG 124
           GL+YSGCPGERPCDGLDACCMKHDACVVAKN+DYLSQECSQSFLNCME FK+S+S PFKG
Sbjct: 63  GLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSRPFKG 122

Query: 125 NKCQIDEVIDVISFVMEAALIAGRVFHKP 154
           NKCQ+DEVIDVISFVMEAALIAG+VFHKP
Sbjct: 123 NKCQVDEVIDVISFVMEAALIAGKVFHKP 151

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BLAST of Lcy03g017600.1 vs. NCBI nr
Match: XP_008453533.1 (PREDICTED: phospholipase A2-alpha [Cucumis melo])

HSP 1 Score: 274.6 bits (701), Expect = 5.1e-70
Identity = 133/156 (85.26%), Postives = 143/156 (91.67%), Query Frame = 0


Query: 1   MTRFQFLKLLLLLLCL---GLASAPAHALNIGVQALDASITMSKECSRKCESEFCSVPPL 60
           M +F  L LLLLLL L    +A  P+HALNIGVQALDASITMSK+CSRKCESEFCSVPPL
Sbjct: 1   MPQFLLLLLLLLLLLLLLPSIAFLPSHALNIGVQALDASITMSKDCSRKCESEFCSVPPL 60

Query: 61  LRYGKYCGLMYSGCPGERPCDGLDACCMKHDACVVAKNNDYLSQECSQSFLNCMEKFKKS 120
           LRYGKYCGL+YSGCPGERPCDGLDACCMKHDACVVAKN+DYLSQECSQSFLNCME FK+S
Sbjct: 61  LRYGKYCGLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRS 120

Query: 121 KSTPFKGNKCQIDEVIDVISFVMEAALIAGRVFHKP 154
           +S PFKGNKCQ+DEVIDVISFVMEAALIAG+VFHKP
Sbjct: 121 RSRPFKGNKCQVDEVIDVISFVMEAALIAGKVFHKP 156

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BLAST of Lcy03g017600.1 vs. NCBI nr
Match: XP_038878664.1 (phospholipase A2-alpha [Benincasa hispida])

HSP 1 Score: 270.8 bits (691), Expect = 7.3e-69
Identity = 127/143 (88.81%), Postives = 136/143 (95.10%), Query Frame = 0


Query: 11  LLLLCLGLASAPAHALNIGVQALDASITMSKECSRKCESEFCSVPPLLRYGKYCGLMYSG 70
           LLLL L +A  P+ ALNIGVQALDASITMSK+CSRKCESEFCSVPPLLRYGKYCGL+YSG
Sbjct: 5   LLLLLLFIAFLPSRALNIGVQALDASITMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSG 64

Query: 71  CPGERPCDGLDACCMKHDACVVAKNNDYLSQECSQSFLNCMEKFKKSKSTPFKGNKCQID 130
           CPGERPCDGLDACCMKHDACVVAKN+DYLSQECSQSF+NCME FK+SKS PFKGNKC++D
Sbjct: 65  CPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFINCMENFKRSKSAPFKGNKCEVD 124

Query: 131 EVIDVISFVMEAALIAGRVFHKP 154
           EVIDVISFVMEAALIAGRVFHKP
Sbjct: 125 EVIDVISFVMEAALIAGRVFHKP 147

[Close]

BLAST of Lcy03g017600.1 vs. NCBI nr
Match: XP_022932135.1 (phospholipase A2-alpha [Cucurbita moschata] >KAG6587708.1 Phospholipase A2-alpha, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021676.1 Phospholipase A2-alpha [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 270.0 bits (689), Expect = 1.2e-68
Identity = 122/142 (85.92%), Postives = 134/142 (94.37%), Query Frame = 0


Query: 12  LLLCLGLASAPAHALNIGVQALDASITMSKECSRKCESEFCSVPPLLRYGKYCGLMYSGC 71
           LLLCL + SAP HALN+GVQALDASIT+SK+CSRKCESEFCSVPPLLRYGKYCGL+YSGC
Sbjct: 11  LLLCLAIVSAPTHALNVGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGC 70

Query: 72  PGERPCDGLDACCMKHDACVVAKNNDYLSQECSQSFLNCMEKFKKSKSTPFKGNKCQIDE 131
           PGERPCDGLDACCM HDACV AKNNDYLS+ECS+SFLNCMEKFK+SKS PFKGNKC+I E
Sbjct: 71  PGERPCDGLDACCMNHDACVAAKNNDYLSEECSKSFLNCMEKFKRSKSAPFKGNKCEISE 130

Query: 132 VIDVISFVMEAALIAGRVFHKP 154
           V++VISFVMEAAL+AGRV HKP
Sbjct: 131 VVEVISFVMEAALLAGRVLHKP 152

[Close]

BLAST of Lcy03g017600.1 vs. NCBI nr
Match: XP_023529843.1 (phospholipase A2-alpha [Cucurbita pepo subsp. pepo])

HSP 1 Score: 270.0 bits (689), Expect = 1.2e-68
Identity = 122/143 (85.31%), Postives = 135/143 (94.41%), Query Frame = 0


Query: 11  LLLLCLGLASAPAHALNIGVQALDASITMSKECSRKCESEFCSVPPLLRYGKYCGLMYSG 70
           LLLLCL + SAP HALN+GVQALDASIT+SK+CSRKCESEFCSVPPLLRYGKYCGL+YSG
Sbjct: 10  LLLLCLAIVSAPTHALNVGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSG 69

Query: 71  CPGERPCDGLDACCMKHDACVVAKNNDYLSQECSQSFLNCMEKFKKSKSTPFKGNKCQID 130
           CPGERPCDGLDACCM HDACV AKNNDYLS+ECS+SFLNCMEKFK+SK+ PFKGNKC+I 
Sbjct: 70  CPGERPCDGLDACCMNHDACVAAKNNDYLSEECSKSFLNCMEKFKRSKAVPFKGNKCEIS 129

Query: 131 EVIDVISFVMEAALIAGRVFHKP 154
           EV++VISFVMEAAL+AGRV HKP
Sbjct: 130 EVVEVISFVMEAALLAGRVLHKP 152

[Close]

BLAST of Lcy03g017600.1 vs. TAIR 10
Match: AT2G06925.1 (Phospholipase A2 family protein )

HSP 1 Score: 223.0 bits (567), Expect = 1.6e-58
Identity = 103/144 (71.53%), Postives = 116/144 (80.56%), Query Frame = 0


Query: 10  LLLLLCLGLASAPAHALNIGVQALDASITMSKECSRKCESEFCSVPPLLRYGKYCGLMYS 69
           ++L   L   S    ALN+GVQ +  SI+++KECSRKCESEFCSVPP LRYGKYCGL+YS
Sbjct: 5   IILFSFLLFFSVSVSALNVGVQLIHPSISLTKECSRKCESEFCSVPPFLRYGKYCGLLYS 64

Query: 70  GCPGERPCDGLDACCMKHDACVVAKNNDYLSQECSQSFLNCMEKFKKSKSTPFKGNKCQI 129
           GCPGERPCDGLD+CCMKHDACV +KNNDYLSQECSQ F+NCM  F + K   FKGNKC  
Sbjct: 65  GCPGERPCDGLDSCCMKHDACVQSKNNDYLSQECSQKFINCMNNFSQKKQPTFKGNKCDA 124

Query: 130 DEVIDVISFVMEAALIAGRVFHKP 154
           DEVIDVIS VMEAALIAG+V  KP
Sbjct: 125 DEVIDVISIVMEAALIAGKVLKKP 148

[Close]

BLAST of Lcy03g017600.1 vs. TAIR 10
Match: AT4G29470.1 (Phospholipase A2 family protein )

HSP 1 Score: 86.3 bits (212), Expect = 2.4e-17
Identity = 42/93 (45.16%), Postives = 58/93 (62.37%), Query Frame = 0


Query: 25  ALNIGVQALDASITMSKECSRKCESEFCSVPPLLRYGKYCGLMYSGCPGERPCDGLDACC 84
           AL + V  L A +   ++CS+ C ++ C+V   +RYGKYCG+ Y GCPGE PCD LD CC
Sbjct: 11  ALGLTVFLLLAVVHSQEKCSKTCIAQKCNVLG-IRYGKYCGIGYFGCPGEPPCDDLDDCC 70

Query: 85  MKHDACVVAKNNDYLSQECSQSFLNCMEKFKKS 118
           M HD CV  K   Y+  +C + F  C+ + K+S
Sbjct: 71  MTHDNCVDLKGMTYV--DCHKQFQRCVNELKQS 100

[Close]

BLAST of Lcy03g017600.1 vs. TAIR 10
Match: AT4G29460.1 (Phospholipase A2 family protein )

HSP 1 Score: 84.7 bits (208), Expect = 6.9e-17
Identity = 39/83 (46.99%), Postives = 52/83 (62.65%), Query Frame = 0


Query: 35  ASITMSKECSRKCESEFCSVPPLLRYGKYCGLMYSGCPGERPCDGLDACCMKHDACVVAK 94
           A ++  ++CS  C ++ C+    +RYGKYCG+ Y GCPGE PCD LDACCM HD CV  K
Sbjct: 21  AVVSSQEKCSNTCIAQNCNSLG-IRYGKYCGIGYFGCPGEPPCDDLDACCMTHDNCVDLK 80

Query: 95  NNDYLSQECSQSFLNCMEKFKKS 118
              Y++  C + F  C+ K  KS
Sbjct: 81  GMTYVN--CHKQFKRCVNKLSKS 100

[Close]

BLAST of Lcy03g017600.1 vs. TAIR 10
Match: AT2G19690.2 (phospholipase A2-beta )

HSP 1 Score: 84.3 bits (207), Expect = 9.0e-17
Identity = 45/123 (36.59%), Postives = 67/123 (54.47%), Query Frame = 0


Query: 37  ITMSKECSRKCESEFCSVPPLLRYGKYCGLMYSGCPGERPCDGLDACCMKHDACVVAKNN 96
           +  S+EC+R C ++ C     +RYGKYCG+ +SGCPGE PCD LDACC  HD CV  + N
Sbjct: 25  VARSEECTRTCIAQNCDTLS-IRYGKYCGIGHSGCPGEEPCDDLDACCKIHDHCV--ELN 84

Query: 97  DYLSQECSQSFLNCME------KFKKSKSTPFKGNKCQIDEVIDVISFVMEAALIAGRV- 153
              +  C + F  C+       K  K+K   F   KC    VI  ++  M+  ++  ++ 
Sbjct: 85  GMTNISCHKKFQRCVNRLSKAIKQSKNKKVGF-STKCPYSVVIPTVNQGMDIGILFSQLA 143

[Close]

BLAST of Lcy03g017600.1 vs. TAIR 10
Match: AT2G19690.1 (phospholipase A2-beta )

HSP 1 Score: 82.8 bits (203), Expect = 2.6e-16
Identity = 44/115 (38.26%), Postives = 62/115 (53.91%), Query Frame = 0


Query: 37  ITMSKECSRKCESEFCSVPPLLRYGKYCGLMYSGCPGERPCDGLDACCMKHDACVVAKNN 96
           +  S+EC+R C ++ C     +RYGKYCG+ +SGCPGE PCD LDACC  HD CV  + N
Sbjct: 25  VARSEECTRTCIAQNCDTLS-IRYGKYCGIGHSGCPGEEPCDDLDACCKIHDHCV--ELN 84

Query: 97  DYLSQECSQSFLNCME------KFKKSKSTPFKGNKCQIDEVIDVISFVMEAALI 146
              +  C + F  C+       K  K+K   F   KC    VI  ++  M+  ++
Sbjct: 85  GMTNISCHKKFQRCVNRLSKAIKQSKNKKVGF-STKCPYSVVIPTVNQGMDIGIL 135

The following BLAST results are available for this feature:

BLAST of Lcy03g017600.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S8N6	2.3e-57	71.53	Phospholipase A2-alpha OS=Arabidopsis thaliana OX=3702 GN=PLA2-ALPHA PE=1 SV=1	[more]
Q10E50	1.3e-44	59.57	Phospholipase A2 homolog 3 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-III ...	[more]
Q9XG81	5.5e-35	50.00	Probable phospholipase A2 homolog 2 OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]
Q9XG80	1.5e-16	41.12	Probable phospholipase A2 homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]
Q8GV50	3.3e-16	45.16	Phospholipase A2-delta OS=Arabidopsis thaliana OX=3702 GN=PLA2-DELTA PE=2 SV=1	[more]

BLAST of Lcy03g017600.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DYM2	1.7e-71	88.59	Phospholipase A2-alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold6...	[more]
A0A1S3BWG7	2.4e-70	85.26	phospholipase A2-alpha OS=Cucumis melo OX=3656 GN=LOC103494216 PE=4 SV=1	[more]
A0A6J1F1D2	6.0e-69	85.92	phospholipase A2-alpha OS=Cucurbita moschata OX=3662 GN=LOC111438456 PE=4 SV=1	[more]
A0A6J1IA72	3.9e-68	84.51	phospholipase A2-alpha OS=Cucurbita maxima OX=3661 GN=LOC111472609 PE=4 SV=1	[more]
A0A0A0LUV2	5.1e-68	84.93	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G433610 PE=4 SV=1	[more]

BLAST of Lcy03g017600.1 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK28548.1	3.5e-71	88.59	phospholipase A2-alpha [Cucumis melo var. makuwa]	[more]
XP_008453533.1	5.1e-70	85.26	PREDICTED: phospholipase A2-alpha [Cucumis melo]	[more]
XP_038878664.1	7.3e-69	88.81	phospholipase A2-alpha [Benincasa hispida]	[more]
XP_022932135.1	1.2e-68	85.92	phospholipase A2-alpha [Cucurbita moschata] >KAG6587708.1 Phospholipase A2-alpha...	[more]
XP_023529843.1	1.2e-68	85.31	phospholipase A2-alpha [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lcy03g017600.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G06925.1	1.6e-58	71.53	Phospholipase A2 family protein	[more]
AT4G29470.1	2.4e-17	45.16	Phospholipase A2 family protein	[more]
AT4G29460.1	6.9e-17	46.99	Phospholipase A2 family protein	[more]
AT2G19690.2	9.0e-17	36.59	phospholipase A2-beta	[more]
AT2G19690.1	2.6e-16	38.26	phospholipase A2-beta	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036444	Phospholipase A2 domain superfamily	GENE3D	1.20.90.10	Phospholipase A2 domain	coord: 24..153 e-value: 6.7E-47 score: 160.4
IPR036444	Phospholipase A2 domain superfamily	SUPERFAMILY	48619	Phospholipase A2, PLA2	coord: 58..152
IPR001211	Phospholipase A2	PANTHER	PTHR11716	PHOSPHOLIPASE A2 FAMILY MEMBER	coord: 11..150
None	No IPR available	PANTHER	PTHR11716:SF87	PHOSPHOLIPASE A2 HOMOLOG 2-RELATED	coord: 11..150
None	No IPR available	CDD	cd04706	PLA2_plant	coord: 37..153 e-value: 3.57368E-60 score: 179.937
IPR033113	Phospholipase A2, histidine active site	PROSITE	PS00118	PA2_HIS	coord: 83..90

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lcy03g017600	Lcy03g017600	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy03g017600.1.exon1	Lcy03g017600.1.exon1	exon
Lcy03g017600.1.exon2	Lcy03g017600.1.exon2	exon
Lcy03g017600.1.exon3	Lcy03g017600.1.exon3	exon
Lcy03g017600.1.exon4	Lcy03g017600.1.exon4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lcy03g017600.1.cds1	Lcy03g017600.1.cds1	CDS
Lcy03g017600.1.cds2	Lcy03g017600.1.cds2	CDS
Lcy03g017600.1.cds3	Lcy03g017600.1.cds3	CDS
Lcy03g017600.1.cds4	Lcy03g017600.1.cds4	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lcy03g017600.1	Lcy03g017600.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050482	arachidonic acid secretion
biological_process	GO:0016042	lipid catabolic process
biological_process	GO:0006644	phospholipid metabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0005794	Golgi apparatus
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0004623	phospholipase A2 activity
molecular_function	GO:0047498	calcium-dependent phospholipase A2 activity
molecular_function	GO:0005543	phospholipid binding