Lcy01g018100.1 (mRNA) Sponge gourd (P93075) v1

BLAST of Lcy01g018100.1 vs. ExPASy Swiss-Prot
Match: O82777 (Subtilisin-like protease SBT3 OS=Solanum lycopersicum OX=4081 GN=sbt3 PE=1 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 6.4e-193
Identity = 363/754 (48.14%), Postives = 501/754 (66.45%), Query Frame = 0

Query: 12  ETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSSKLIHSYKH 71
           +   YIVH+D + MP  F  HH W+S+T+ S+       ++ SS      + KL++SY +
Sbjct: 23  QRSTYIVHLDKSLMPNVFTDHHHWHSSTIDSI-----KASVPSSVDRFHSAPKLVYSYDN 82

Query: 72  AINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSGLLPISNYGSD 131
            ++GFSA L+  EL AL+K PG++S+ +D +V+  TTH+S FL L+ +SGL P S  G D
Sbjct: 83  VLHGFSAVLSKDELAALKKLPGFISAYKDRTVEPHTTHTSDFLKLNPSSGLWPASGLGQD 142

Query: 132 VIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGARFFNKGLIA 191
           VI+ V+D+G+WPES SF DDGM EIP RWKG C+ G  F  S+CN+KLIGA +FNKG++A
Sbjct: 143 VIVAVLDSGIWPESASFQDDGMPEIPKRWKGICKPGTQFNASMCNRKLIGANYFNKGILA 202

Query: 192 KFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRARVAIYKAVW 251
             P + I+MNS RDT+GHGTH ++  AG++ K  S FGY  G ARGVAPRAR+A+YK  +
Sbjct: 203 NDPTVNITMNSARDTDGHGTHCASITAGNFAKGVSHFGYAPGTARGVAPRARLAVYKFSF 262

Query: 252 AEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAMERGIFVATSAGN 311
            EG + SD+IAA+D A+ DGVD+IS+S G   +PLYED I++A++ AM +G+ V+ SAGN
Sbjct: 263 NEGTFTSDLIAAMDQAVADGVDMISISYGYRFIPLYEDAISIASFGAMMKGVLVSASAGN 322

Query: 312 NGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLNSAMTVSPLPIVY 371
            GP +G+++NG+PW+L VA+G  DR F GT+TL NG+ + G SLFP  + +  S  P++Y
Sbjct: 323 RGPGIGSLNNGSPWILCVASGHTDRTFAGTLTLGNGLKIRGWSLFPARAFVRDS--PVIY 382

Query: 372 ---MGRCHNSKKLKRV---GYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISNISDWDN 431
              +  C + + L +V      IV+C+D  + S  +++  +  A++   +FIS       
Sbjct: 383 NKTLSDCSSEELLSQVENPENTIVICDDNGDFSDQMRI--ITRARLKAAIFISEDPGVFR 442

Query: 432 LIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSRGPSESC 491
               P P + ++   G  + +Y+K S  P A + F +T L TKPAP VA  S+RGPS S 
Sbjct: 443 SATFPNPGVVVNKKEGKQVINYVKNSVTPTATITFQETYLDTKPAPVVAASSARGPSRSY 502

Query: 492 PFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVL-SGTSMSCPHAAGVAALLKG 551
             + KPDI+APG  ILA++P NV AT + +  + S   +L SGTSM+ PHAAG+AA+LK 
Sbjct: 503 LGISKPDILAPGVLILAAYPPNVFATSIGTNILLSTDYILESGTSMAAPHAAGIAAMLKA 562

Query: 552 AHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMDPGLVY 611
           AHP WS +AIRSAMMTTAD LDNTR PIKD  D NK ATPL MG+GHV+PN+A+DPGLVY
Sbjct: 563 AHPEWSPSAIRSAMMTTADPLDNTRKPIKD-SDNNKAATPLDMGAGHVDPNRALDPGLVY 622

Query: 612 DMGIQDYVNLLCALNYTKNQIQTITRST-SNNCENPSLDLNYPSFIIIVNASDSKATREI 671
           D   QDYVNLLC+LN+T+ Q +TI RS+ S+NC NPS DLNYPSFI + +   +    E 
Sbjct: 623 DATPQDYVNLLCSLNFTEEQFKTIARSSASHNCSNPSADLNYPSFIALYSIEGNFTLLE- 682

Query: 672 SREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKI-----EGR 731
            ++F R VTN+G+   TYKAKL A     ++V P+ L FK KN+K S+ L I     EG+
Sbjct: 683 -QKFKRTVTNVGKGAATYKAKLKAPKNSTISVSPQILVFKNKNEKQSYTLTIRYIGDEGQ 742

Query: 732 ARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSL 753
           +R         G ++WVE  G H V+SPIV + +
Sbjct: 743 SR-------NVGSITWVEQNGNHSVRSPIVTSPI 757

BLAST of Lcy01g018100.1 vs. ExPASy Swiss-Prot
Match: Q9FHA4 (Subtilisin-like protease SBT1.9 OS=Arabidopsis thaliana OX=3702 GN=SBT1.9 PE=2 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 3.2e-168
Identity = 337/766 (43.99%), Postives = 471/766 (61.49%), Query Frame = 0

Query: 4   FWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSS 63
           F++     ET  YI+HMDL+A P PF+ H SW+S TL+SV+ N +               
Sbjct: 13  FFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVITNRK--------------P 72

Query: 64  KLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSGLL 123
           K+I++Y  +++GFSA LT SEL+ L+  PGYVS  +D  V++ TT S  F+GL+S SG  
Sbjct: 73  KIIYAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNSTSGTW 132

Query: 124 PISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGAR 183
           P+SNYG+ ++IG++DTG+WP+S SF+DDG+  +PS+WKG CE  +    SLCNKKLIGA+
Sbjct: 133 PVSNYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFNS---SSLCNKKLIGAK 192

Query: 184 FFNKGLIAKFPNITIS----MNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 243
            FNKGL A  P++  +     +S  DT GHGTH +  AAG++VK AS+F Y  G A G+A
Sbjct: 193 VFNKGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTASGIA 252

Query: 244 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGV--------NGVPLYEDPI 303
           P A +AIYKA W EG Y SDVIAAID AI DGV VISLSLG+        +G  L  DPI
Sbjct: 253 PHAHLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLENDPI 312

Query: 304 AVATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVL 363
           AVA++AA+++G+FV TS GN+GP   ++ NGAPW++ V AGT+ R F GT+T  N VS  
Sbjct: 313 AVASFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNRVSFS 372

Query: 364 GSSLFPLNSAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKV 423
             SLFP        P+  +  G   N    K +  +IVVC   +  ++  ++  ++S   
Sbjct: 373 FPSLFPGEFPSVQFPVTYIESGSVEN----KTLANRIVVC--NENINIGSKLHQIRSTGA 432

Query: 424 AVGVFISN-ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGS-SDPKARVNFHKTILGTKP 483
           A  V I++ + +  + I+  FP  F+       I+ Y   + ++  A++ F KT++GTKP
Sbjct: 433 AAVVLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEFRKTVIGTKP 492

Query: 484 APSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTS 543
           AP V  YSSRGP  S P +LKPDI+APG  IL++WP     T   + P++S FN+L+GTS
Sbjct: 493 APEVGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFSGFNLLTGTS 552

Query: 544 MSCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGS 603
           M+ PH AGVAAL+K  HP WS +AI+SA+MTTA  LDN                PLA+G+
Sbjct: 553 MAAPHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN----------------PLAVGA 612

Query: 604 GHVNPNKAMDPGLVYDMGIQDYVNLLC-ALNYTKNQIQTITRST-SNNCENPSLDLNYPS 663
           GHV+ NK ++PGL+YD   QD++N LC     ++  I  ITRS  S+ C+ PS  LNYPS
Sbjct: 613 GHVSTNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKKPSPYLNYPS 672

Query: 664 FIIIVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKND 723
            I    +  S       + F R +TN+GE   +Y  ++  + GL V V+PKKL F  KN+
Sbjct: 673 IIAYFTSDQSS-----PKIFKRTLTNVGEAKRSYIVRVRGLKGLNVVVEPKKLMFSEKNE 732

Query: 724 KLSFKLKIEGRARVKRETDVVFGYLSWV-EVGGKHIVQSPIVVTSL 753
           KLS+ +++E    +  + +VV+G +SWV E   +  V   +V TSL
Sbjct: 733 KLSYTVRLESPRGL--QENVVYGLVSWVDEDEAEFEVSCSVVATSL 732

BLAST of Lcy01g018100.1 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 1.3e-153
Identity = 315/757 (41.61%), Postives = 443/757 (58.52%), Query Frame = 0

Query: 11  TETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSSKLIHSYK 70
           T  + YI+ ++ +  P+ F +HH WY++ L+S                    S L+++Y 
Sbjct: 25  TAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS-------------------ESSLLYTYT 84

Query: 71  HAINGFSASLTPSELEALQKSPGYVSSV-RDSSVQVDTTHSSHFLGLSSNSGLLPISNYG 130
            + +GFSA L  +E ++L  S   +  +  D    + TT +  FLGL+S  G+  + +  
Sbjct: 85  TSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSS 144

Query: 131 SDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGARFFNKGL 190
           + VIIGV+DTGVWPES SF+D  M EIPS+WKGECESG+ F   LCNKKLIGAR F+KG 
Sbjct: 145 NGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGF 204

Query: 191 -IAKFPNITISMNST--RDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRARVAI 250
            +A     +    S   RD +GHGTHTSTTAAGS V+ ASF GY +G ARG+A RARVA 
Sbjct: 205 QMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVAT 264

Query: 251 YKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAMERGIFVA 310
           YK  W+ G + SD++AA+D AILDGVDV+SLSLG    P Y D IA+  ++AMERG+FV+
Sbjct: 265 YKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVS 324

Query: 311 TSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLNSAMTVSP 370
            SAGN+GP   +V N APWV+ V AGT+DRDF     L NG  + G SL+     M   P
Sbjct: 325 CSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS-GVGMGTKP 384

Query: 371 LPIVYMGRCHNSKKLKRVGY--------KIVVCEDKDEHSLSLQVDNVQSAKVAVG---V 430
           L +VY     +S  L   G         KIVVC    +  ++ +V+     + A G   +
Sbjct: 385 LELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVC----DRGVNARVEKGAVVRDAGGLGMI 444

Query: 431 FISNISDWDNLIQTP--FPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSV 490
             +  +  + L+      P+I +    G+++++Y+K  S P A + F  T+L  KP+P V
Sbjct: 445 MANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVV 504

Query: 491 ARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCP 550
           A +SSRGP+   P +LKPD++ PG  ILA W   +  T ++     ++FN++SGTSMSCP
Sbjct: 505 AAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCP 564

Query: 551 HAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVN 610
           H +G+A LLK AHP WS +AI+SA+MTTA VLDNT  P+ D  D N L+ P A GSGHV+
Sbjct: 565 HISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAAD-NSLSNPYAHGSGHVD 624

Query: 611 PNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLD---LNYPSFII 670
           P KA+ PGLVYD+  ++Y+  LC+L+YT + I  I +  S NC     D   LNYPSF +
Sbjct: 625 PQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSV 684

Query: 671 IVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLS 730
           +         + + R +TR VTN+G   + YK  +     + ++VKP KL FK   +K  
Sbjct: 685 LFGG------KRVVR-YTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKR 744

Query: 731 FKLKIEGRARVKRETDVVFGYLSWVEVGGKHIVQSPI 748
           + +    +  V       FG ++W     +H V+SP+
Sbjct: 745 YTVTFVSKKGVSMTNKAEFGSITW--SNPQHEVRSPV 747

BLAST of Lcy01g018100.1 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 3.7e-148
Identity = 314/757 (41.48%), Postives = 449/757 (59.31%), Query Frame = 0

Query: 16  YIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSSKLIHSYKHAING 75
           YIVHM  + MP  F  H +WY ++L S+ D                S++L+++Y++AI+G
Sbjct: 32  YIVHMAKSQMPSSFDLHSNWYDSSLRSISD----------------SAELLYTYENAIHG 91

Query: 76  FSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSG-LLPISNYGSDVII 135
           FS  LT  E ++L   PG +S + +   ++ TT +  FLGL  ++  L P +   SDV++
Sbjct: 92  FSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVV 151

Query: 136 GVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGARFFNKGLIAKFP 195
           GV+DTGVWPES S++D+G   IPS WKG CE+G +F  SLCN+KLIGARFF +G  +   
Sbjct: 152 GVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMG 211

Query: 196 NITIS--MNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRARVAIYKAVWA 255
            I  S    S RD +GHGTHTS+TAAGS V+ AS  GY SG ARG+APRARVA+YK  W 
Sbjct: 212 PIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWL 271

Query: 256 EGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAMERGIFVATSAGNN 315
            G + SD++AAID AI D V+V+S+SLG      Y D +A+  +AAMERGI V+ SAGN 
Sbjct: 272 GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNA 331

Query: 316 GPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLNSAMTVSPLPIVYM 375
           GP   ++ N APW+  V AGT+DRDF     L NG +  G SLF    A+    LP +Y 
Sbjct: 332 GPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK-GEALPDKLLPFIYA 391

Query: 376 GRCHNSKK----------LKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN-IS 435
           G   N+             ++V  KIV+C+     +  +Q  +V  A   VG+ ++N  +
Sbjct: 392 GNASNATNGNLCMTGTLIPEKVKGKIVMCD--RGINARVQKGDVVKAAGGVGMILANTAA 451

Query: 436 DWDNLIQTP--FPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 495
           + + L+      P+  +    G+II+ Y+    +P A ++   T++G KP+P VA +SSR
Sbjct: 452 NGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSR 511

Query: 496 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 555
           GP+   P +LKPD++APG  ILA+W      T + S     +FN++SGTSMSCPH +G+A
Sbjct: 512 GPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLA 571

Query: 556 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 615
           ALLK  HP WS AAIRSA+MTTA        P+ D+    K +TP   G+GHV+P  A +
Sbjct: 572 ALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIA-TGKPSTPFDHGAGHVSPTTATN 631

Query: 616 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPS-----LDLNYPSFIIIVNA 675
           PGL+YD+  +DY+  LCALNYT  QI++++R   N   +PS      DLNYPSF + V+ 
Sbjct: 632 PGLIYDLTTEDYLGFLCALNYTSPQIRSVSR--RNYTCDPSKSYSVADLNYPSFAVNVDG 691

Query: 676 SDSKATREISREFTRMVTNIGEKGTTYKAKLIA-MDGLRVTVKPKKLKFKWKNDKLSFKL 735
                    + ++TR VT++G  G TY  K+ +   G++++V+P  L FK  N+K S+ +
Sbjct: 692 VG-------AYKYTRTVTSVGGAG-TYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTV 751

Query: 736 KIEGRARVKRETDVVFGYLSWVEVGGKHIVQSPIVVT 751
                +  K      FG + W +  GKH+V SP+ ++
Sbjct: 752 TFTVDSS-KPSGSNSFGSIEWSD--GKHVVGSPVAIS 755

BLAST of Lcy01g018100.1 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 5.3e-147
Identity = 319/766 (41.64%), Postives = 443/766 (57.83%), Query Frame = 0

Query: 14  ENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSSKLIHSYKHAI 73
           + +I  +D  +MP  F +H+ WYS   +                     S+++H Y    
Sbjct: 26  KTFIFRIDGGSMPSIFPTHYHWYSTEFAE-------------------ESRIVHVYHTVF 85

Query: 74  NGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSGLLPISNYGSDVI 133
           +GFSA +TP E + L+  P  ++   D   ++ TT S  FLGL +  GL   S+YGSDVI
Sbjct: 86  HGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDVI 145

Query: 134 IGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGARFFNKGLIAKF 193
           IGV DTG+WPE  SF+D  +  IP RW+G CESGA F    CN+K+IGARFF KG  A  
Sbjct: 146 IGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAV 205

Query: 194 ---PNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRARVAIYKAV 253
               N T+   S RD +GHGTHTS+TAAG +  +AS  GY SG+A+GVAP+AR+A YK  
Sbjct: 206 IGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVC 265

Query: 254 WAE-GNYVSDVIAAIDHAILDGVDVISLSL-GVNGV--PLYEDPIAVATYAAMERGIFVA 313
           W + G   SD++AA D A+ DGVDVIS+S+ G +G+  P Y DPIA+ +Y A  +GIFV+
Sbjct: 266 WKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVS 325

Query: 314 TSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLF---PLNSAMT 373
           +SAGN GP   +V N APWV  V A T+DR+F     L +G  + G SL+   PLN  M 
Sbjct: 326 SSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNGRM- 385

Query: 374 VSPLPIVYMGR---------CHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVG 433
               P+VY G+           N+   K+V  KIV+C+      ++  +  V      VG
Sbjct: 386 ---FPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGL--VVKKAGGVG 445

Query: 434 VFISN-ISDWDNLIQTP--FPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAP 493
           + ++N  S+ + L+      P+  +    G+ IK Y     +P A ++F  TI+G KPAP
Sbjct: 446 MILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAP 505

Query: 494 SVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMS 553
            +A +S RGP+   P +LKPD++APG  ILA+W   V  T + S P  ++FN+LSGTSM+
Sbjct: 506 VIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMA 565

Query: 554 CPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGH 613
           CPH +G AALLK AHP WS A IRSAMMTT +++DN+   + D     K ATP   GSGH
Sbjct: 566 CPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLID-ESTGKSATPYDYGSGH 625

Query: 614 VNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSN--NCENPSL-DLNYPSF 673
           +N  +AM+PGLVYD+   DY+  LC++ Y    IQ ITR+         PS  +LNYPS 
Sbjct: 626 LNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPS- 685

Query: 674 IIIVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDK 733
             I     +     +S+   R  TN+G+    Y+A++ +  G+ VTVKP +L F     +
Sbjct: 686 --ITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKR 745

Query: 734 LSFKLKIEGRAR--VKRETDVVFGYLSWVEVGGKHIVQSPIVVTSL 753
            S+ + +    R  V  ET  VFG ++W + GGKH+V+SPIVVT +
Sbjct: 746 RSYAVTVTVNTRNVVLGETGAVFGSVTWFD-GGKHVVRSPIVVTQM 761

BLAST of Lcy01g018100.1 vs. ExPASy TrEMBL
Match: A0A6J1HH07 (subtilisin-like protease SBT1.9 OS=Cucurbita moschata OX=3662 GN=LOC111462861 PE=3 SV=1)

HSP 1 Score: 1238.4 bits (3203), Expect = 0.0e+00
Identity = 630/763 (82.57%), Postives = 689/763 (90.30%), Query Frame = 0

Query: 1   MAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDNSRLGTMASSSSSS 60
           M IF    T T+T+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD S L T  +SSSSS
Sbjct: 14  MVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT-TTSSSSS 73

Query: 61  SLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSN 120
           SL SKLIH YKHAINGF+ASLTPS+L+AL+ SPGYVSS+RDSSV+ DTTHSS+FL LS N
Sbjct: 74  SLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTTHSSNFLALSPN 133

Query: 121 SGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKL 180
           SGLLPISNYGSDVIIG VDTGVWPES SFNDDG+S+IPSRWKGECESG HF  SLCNKKL
Sbjct: 134 SGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNASLCNKKL 193

Query: 181 IGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 240
           IG RFFNKGLI+KFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG G ARGVA
Sbjct: 194 IGGRFFNKGLISKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVA 253

Query: 241 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAM 300
           PRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG++GVPLYEDP+A+AT+AAM
Sbjct: 254 PRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAM 313

Query: 301 ERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLN 360
           ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTITL NGVSVLGSSLFPLN
Sbjct: 314 ERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLGSSLFPLN 373

Query: 361 SAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN 420
           SA+ +SPLPIV+MG+CHN KKLKRVG+KIVVCED DE+SL LQVDNV+SAK+AVGVFISN
Sbjct: 374 SAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKIAVGVFISN 433

Query: 421 ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 480
           ISDWDNLIQT FPSIFL+ Y GN+IKDYIK SS+PKARVNFHKTI+GTKPAPSVARYSSR
Sbjct: 434 ISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAPSVARYSSR 493

Query: 481 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 540
           GPSESCPFVLKPDIMAPGDAILASWPQNVAATDV SRPIYSKFNVLSGTSM+CPHAAGVA
Sbjct: 494 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSGTSMACPHAAGVA 553

Query: 541 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 600
           ALLKGAHP WS AAIRSAMMTTAD++DNT+T IKDLG++NK+ATPLAMGSGHVNPNKA+D
Sbjct: 554 ALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAID 613

Query: 601 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSKA 660
           PGL+YDMGI+DY NLLCALNYTKNQIQTITRSTSN+CE   LDLNYPSFI+ VNASDS+ 
Sbjct: 614 PGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPSFIMTVNASDSQT 673

Query: 661 TR-EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGR 720
            R E+SREF R VTNIGEKG TY+AK+  M GL VTV+P KLKFK KN  LSFKLKI G 
Sbjct: 674 GRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLSFKLKIRGH 733

Query: 721 ARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSLKSKVKSHRN 762
             +KRE+DVVFGYL+WVEVGG H VQSPIVV ++ + ++SH N
Sbjct: 734 VSIKRESDVVFGYLNWVEVGGGHRVQSPIVV-AVVAGLRSHWN 774

BLAST of Lcy01g018100.1 vs. ExPASy TrEMBL
Match: A0A6J1K9W2 (subtilisin-like protease SBT1.9 OS=Cucurbita maxima OX=3661 GN=LOC111491986 PE=3 SV=1)

HSP 1 Score: 1233.0 bits (3189), Expect = 0.0e+00
Identity = 628/756 (83.07%), Postives = 681/756 (90.08%), Query Frame = 0

Query: 1   MAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDNSRLGTMASSSSSS 60
           M I     T TET+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD S L T  ++SSSS
Sbjct: 14  MVILLARSTLTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT--TTSSSS 73

Query: 61  SLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSN 120
           SL SKLIH YKHAINGF+A+LTPS+L+AL+ SPGYVSSVRDSSV+ DTTHSS+FL L+ N
Sbjct: 74  SLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNFLALTPN 133

Query: 121 SGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKL 180
           SGLLPISNYGSDVIIG VDTGVWPES SFND+ +S+IPSRWKGECESG HF  SLCNKKL
Sbjct: 134 SGLLPISNYGSDVIIGFVDTGVWPESESFNDEAISKIPSRWKGECESGTHFNASLCNKKL 193

Query: 181 IGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 240
           IG RFFNKGLIAKFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG G ARGVA
Sbjct: 194 IGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVA 253

Query: 241 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAM 300
           PRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG++GVPLYEDP+A+AT+AAM
Sbjct: 254 PRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAM 313

Query: 301 ERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLN 360
           ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLF LN
Sbjct: 314 ERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFVLN 373

Query: 361 SAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN 420
           SA+ +SPLPIV+MG+CHN KKLKRVG+KIVVCED DE+SL LQVDNVQSAK+AVGVFISN
Sbjct: 374 SAIVLSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQSAKIAVGVFISN 433

Query: 421 ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 480
           ISDWDNLIQT FPSIFL+ Y GN+IKDYI  SS+PKARVNFHKTI+GTKPAPSVARYSSR
Sbjct: 434 ISDWDNLIQTSFPSIFLNAYHGNVIKDYIMRSSNPKARVNFHKTIIGTKPAPSVARYSSR 493

Query: 481 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 540
           GPSESCP VLKPDIMAPGDAILASWPQ VAATDV SRPIYSKFNVLSGTSM+CPHAAGVA
Sbjct: 494 GPSESCPLVLKPDIMAPGDAILASWPQKVAATDVYSRPIYSKFNVLSGTSMACPHAAGVA 553

Query: 541 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 600
           ALLKGAHP WS AAIRSAMMTTADV+DNT+T IKDLG++NK+ATPLAMGSGHVNPNKA+D
Sbjct: 554 ALLKGAHPGWSPAAIRSAMMTTADVVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAID 613

Query: 601 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSKA 660
           PGL+YD+GI+DY NLLCALNYTKNQIQTITRS SN+CE PSLDLNYPSFII VNASDS++
Sbjct: 614 PGLIYDIGIEDYTNLLCALNYTKNQIQTITRSPSNDCEKPSLDLNYPSFIITVNASDSES 673

Query: 661 -TREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGR 720
             REISREF R VTNIGEKG TY+AK+  M GL VTV+PK LKFK KN  LSFKLKI G 
Sbjct: 674 GRREISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKMLKFKRKNQNLSFKLKIRGH 733

Query: 721 ARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSLKS 755
             VKRE+DVVFGYL+WVEVGG H VQSPIVV  L+S
Sbjct: 734 VSVKRESDVVFGYLNWVEVGGGHRVQSPIVVAGLRS 767

BLAST of Lcy01g018100.1 vs. ExPASy TrEMBL
Match: A0A5A7V589 (Subtilisin-like protease SBT1.9 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00840 PE=3 SV=1)

HSP 1 Score: 1183.3 bits (3060), Expect = 0.0e+00
Identity = 595/752 (79.12%), Postives = 660/752 (87.77%), Query Frame = 0

Query: 3   IFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLS 62
           IFWL    TET NYIVHM+ AAMPKPFAS HSWYSAT+SSVL +S   + +SSSSSSS  
Sbjct: 14  IFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSSSSSSSSSSSFP 73

Query: 63  SKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSGL 122
           SKLIH+Y HAI+GF ASLTPS+LEAL+ SPGY+SSV DSSV VDTTHSSHFLGLSSN GL
Sbjct: 74  SKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHGL 133

Query: 123 LPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGA 182
           LPIS YGSDVIIG VDTG+WP+S SFNDDGMSEIPSRWKGECES  HF  S CN KLIGA
Sbjct: 134 LPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFNVSFCNNKLIGA 193

Query: 183 RFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRA 242
           RFFNKGLIA+FPN TISMNSTRDT GHGTHTST AAGSYVKEASFFGYG G ARGVAPRA
Sbjct: 194 RFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFGYGRGTARGVAPRA 253

Query: 243 RVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAMERG 302
           RVAIYKA+W EGN VSDV+AAID AI DGVDVISLS+G++G+PLY DP+++AT+AA+ERG
Sbjct: 254 RVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYNDPVSIATFAAVERG 313

Query: 303 IFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLNSAM 362
           IFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITL+NGVSVLGSSLFPLN+ M
Sbjct: 314 IFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNTTM 373

Query: 363 TVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISNISD 422
            +SPLPIV+MG C N KKLKR+GYKIVVCED D +SL+ QVDNVQ+AKV +G+FISNI D
Sbjct: 374 GLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAKVTLGIFISNIFD 433

Query: 423 WDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSRGPS 482
           WDNLIQTPFPSIF++PY GNIIKDYI  SSDPKA VNFHKTILGTKPAP+VARYSSRGPS
Sbjct: 434 WDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKPAPTVARYSSRGPS 493

Query: 483 ESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVAALL 542
           +SCPFVLKPDIMAPGDAILASWPQN+ A DVNS PIY+KFNV+SGTSMSCPHAAGVAALL
Sbjct: 494 QSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSMSCPHAAGVAALL 553

Query: 543 KGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMDPGL 602
           KGAHP+WS AAIRSAMMTTADV+DNT+T IKD+G+ NK ATPLAMGSGHVNPNKA+DP L
Sbjct: 554 KGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSGHVNPNKAVDPDL 613

Query: 603 VYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSK-ATR 662
           +YD+GIQDYVN+LCALNYT+NQI+ ITRS SNNCENPSLDLNYPSFIII N+SDSK   R
Sbjct: 614 IYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIIIANSSDSKTGKR 673

Query: 663 EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGRARV 722
           +I  EF R +T IGE   TY+AKL  M G +V VKP+ L+FK KN KLSF+LKI G A  
Sbjct: 674 KILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQKLSFELKIAGSA-- 733

Query: 723 KRETDVVFGYLSWVEVGGKHIVQSPIVVTSLK 754
             E++VVFGYLSW EVGG+HIVQSPIVV  ++
Sbjct: 734 -EESNVVFGYLSWAEVGGRHIVQSPIVVAGMR 762

BLAST of Lcy01g018100.1 vs. ExPASy TrEMBL
Match: A0A1S3BBC3 (subtilisin-like protease SBT1.9 OS=Cucumis melo OX=3656 GN=LOC103487840 PE=3 SV=1)

HSP 1 Score: 1183.3 bits (3060), Expect = 0.0e+00
Identity = 595/752 (79.12%), Postives = 660/752 (87.77%), Query Frame = 0

Query: 3   IFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLS 62
           IFWL    TET NYIVHM+ AAMPKPFAS HSWYSAT+SSVL +S   + +SSSSSSS  
Sbjct: 14  IFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSSSSSSSSSSSFP 73

Query: 63  SKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSGL 122
           SKLIH+Y HAI+GF ASLTPS+LEAL+ SPGY+SSV DSSV VDTTHSSHFLGLSSN GL
Sbjct: 74  SKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFLGLSSNHGL 133

Query: 123 LPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGA 182
           LPIS YGSDVIIG VDTG+WP+S SFNDDGMSEIPSRWKGECES  HF  S CN KLIGA
Sbjct: 134 LPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFNVSFCNNKLIGA 193

Query: 183 RFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRA 242
           RFFNKGLIA+FPN TISMNSTRDT GHGTHTST AAGSYVKEASFFGYG G ARGVAPRA
Sbjct: 194 RFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFGYGRGTARGVAPRA 253

Query: 243 RVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAMERG 302
           RVAIYKA+W EGN VSDV+AAID AI DGVDVISLS+G++G+PLY DP+++AT+AA+ERG
Sbjct: 254 RVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYNDPVSIATFAAVERG 313

Query: 303 IFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLNSAM 362
           IFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITL+NGVSVLGSSLFPLN+ M
Sbjct: 314 IFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSVLGSSLFPLNTTM 373

Query: 363 TVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISNISD 422
            +SPLPIV+MG C N KKLKR+GYKIVVCED D +SL+ QVDNVQ+AKV +G+FISNI D
Sbjct: 374 GLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAKVTLGIFISNIFD 433

Query: 423 WDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSRGPS 482
           WDNLIQTPFPSIF++PY GNIIKDYI  SSDPKA VNFHKTILGTKPAP+VARYSSRGPS
Sbjct: 434 WDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKPAPTVARYSSRGPS 493

Query: 483 ESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVAALL 542
           +SCPFVLKPDIMAPGDAILASWPQN+ A DVNS PIY+KFNV+SGTSMSCPHAAGVAALL
Sbjct: 494 QSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSMSCPHAAGVAALL 553

Query: 543 KGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMDPGL 602
           KGAHP+WS AAIRSAMMTTADV+DNT+T IKD+G+ NK ATPLAMGSGHVNPNKA+DP L
Sbjct: 554 KGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSGHVNPNKAVDPDL 613

Query: 603 VYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSK-ATR 662
           +YD+GIQDYVN+LCALNYT+NQI+ ITRS SNNCENPSLDLNYPSFIII N+SDSK   R
Sbjct: 614 IYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIIIANSSDSKTGKR 673

Query: 663 EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGRARV 722
           +I  EF R +T IGE   TY+AKL  M G +V VKP+ L+FK KN KLSF+LKI G A  
Sbjct: 674 KILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQKLSFELKIAGSA-- 733

Query: 723 KRETDVVFGYLSWVEVGGKHIVQSPIVVTSLK 754
             E++VVFGYLSW EVGG+HIVQSPIVV  ++
Sbjct: 734 -EESNVVFGYLSWAEVGGRHIVQSPIVVAGMR 762

BLAST of Lcy01g018100.1 vs. ExPASy TrEMBL
Match: A0A6J1BPR9 (subtilisin-like protease SBT1.9 OS=Momordica charantia OX=3673 GN=LOC111004540 PE=3 SV=1)

HSP 1 Score: 1177.2 bits (3044), Expect = 0.0e+00
Identity = 603/768 (78.52%), Postives = 669/768 (87.11%), Query Frame = 0

Query: 1   MAIFW--LPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSS 60
           +AIFW  LP   TET+NYIVHMDLAAMPKPFA+HH+WYSATLSSVLDN RLG        
Sbjct: 18  IAIFWFLLPSASTETDNYIVHMDLAAMPKPFATHHTWYSATLSSVLDNPRLG-------- 77

Query: 61  SSLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSS 120
             L SKLIH+Y HAINGFSAS+TPSEL+ALQKSPGYVSSV DSS+QVDTTHSSHFLGLSS
Sbjct: 78  -MLPSKLIHTYNHAINGFSASITPSELKALQKSPGYVSSVPDSSLQVDTTHSSHFLGLSS 137

Query: 121 NSGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKK 180
           +SG+L +SNYGSDVIIG VDTGVW ES SF+D+GM EIPSRWKG+CESG H     CNKK
Sbjct: 138 DSGVL-LSNYGSDVIIGFVDTGVWAESESFSDEGMPEIPSRWKGKCESGTH-----CNKK 197

Query: 181 LIGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGV 240
           LIGARFFN+GLIAKFPN+TISMN T DTNGHGTHT+ TAAG YVK+ASFFGYG G ARGV
Sbjct: 198 LIGARFFNRGLIAKFPNVTISMNYTNDTNGHGTHTAATAAGGYVKDASFFGYGRGTARGV 257

Query: 241 APRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAA 300
           APRARVAIYKA+W EGNYVSDVIAAID AILD VDV+SLSLG++G  LYEDP+A+AT+AA
Sbjct: 258 APRARVAIYKAIWEEGNYVSDVIAAIDQAILDSVDVLSLSLGLDGALLYEDPVAIATFAA 317

Query: 301 MERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPL 360
           +ERGI V TSAGN GPQLGTVHNGAPWVLNVAAGTMDRDFGGTI L NGVSVLGSSLFPL
Sbjct: 318 VERGILVTTSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIRLGNGVSVLGSSLFPL 377

Query: 361 NSAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDE---HSLSLQVDNVQSAKVAVGV 420
           NS M  S LP+V+MG C+N KKLK+VG+KIVVCEDKDE   +SLSLQVDNV++AKV VGV
Sbjct: 378 NSGMATSKLPLVFMGGCYNLKKLKKVGHKIVVCEDKDEYNSYSLSLQVDNVETAKVGVGV 437

Query: 421 FISNISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVAR 480
           FISN+SDWDN +QT FPSIFLSP  GNIIK YI+ SS+PKARV+FHKTI G KPAPSVAR
Sbjct: 438 FISNLSDWDNSVQTSFPSIFLSPSNGNIIKHYIRTSSNPKARVSFHKTIFGRKPAPSVAR 497

Query: 481 YSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHA 540
           YSSRGP+ESCPFVLKPDIMAPGDAILASWP  VAATDVNSRPIYSKFNVLSGTSMSCPHA
Sbjct: 498 YSSRGPAESCPFVLKPDIMAPGDAILASWPLKVAATDVNSRPIYSKFNVLSGTSMSCPHA 557

Query: 541 AGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPN 600
           AGVAALLK AHPRWS AAIRSAMMTTADV+DNT+TPIKDLG++NKLATPLAMGSGHVNPN
Sbjct: 558 AGVAALLKAAHPRWSPAAIRSAMMTTADVVDNTQTPIKDLGEKNKLATPLAMGSGHVNPN 617

Query: 601 KAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNAS 660
           KA+DPGL+YDM IQDYVN+LCALNY KNQIQTITRS SNNC+NPSLDLNYPSFI+IVNAS
Sbjct: 618 KAVDPGLIYDMEIQDYVNVLCALNYNKNQIQTITRSASNNCKNPSLDLNYPSFIVIVNAS 677

Query: 661 DSKATREISREFTRMVTNIGEKGTTYKAKLIAM--DGLRVTVKPKKLKFKWKNDKLSFKL 720
           DS    EISREF R VTN+ E+ TTY+AKL  M  +G+RVTVKP KL+FK KN KLSF +
Sbjct: 678 DSNTEMEISREFKRTVTNVVEEATTYEAKLTPMNGNGVRVTVKPHKLEFKGKNHKLSFTV 737

Query: 721 KIE-GRARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSLKSKVKSHR 761
           K++ G   V+R+ +VVF YLSWVEVGG H+ +SPIVVT L   ++SH+
Sbjct: 738 KVQLGHWIVQRDPNVVFCYLSWVEVGGGHVARSPIVVTDLNFMLRSHQ 770

BLAST of Lcy01g018100.1 vs. NCBI nr
Match: XP_023546329.1 (subtilisin-like protease SBT1.9 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1245.0 bits (3220), Expect = 0.0e+00
Identity = 631/756 (83.47%), Postives = 685/756 (90.61%), Query Frame = 0

Query: 1   MAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDNSRLGTMASSSSSS 60
           M IF    T TET+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD S L T  ++SSSS
Sbjct: 14  MVIFLARSTVTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT--TTSSSS 73

Query: 61  SLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSN 120
           SL SKLIH YKHAINGF+A+LTPS+L+AL+ SPGYVSSVRDSSV+ DTTHSS+FL LS N
Sbjct: 74  SLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNFLALSPN 133

Query: 121 SGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKL 180
           SGLLPISNYGSDVIIG VDTGVWPES SFNDDG+S+IPSRWKGECESG HF  SLCNKKL
Sbjct: 134 SGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNASLCNKKL 193

Query: 181 IGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 240
           IG RFFNKGLIAKFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG G ARGVA
Sbjct: 194 IGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVA 253

Query: 241 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAM 300
           PRARVAIYKA+W EGN VSDVIAAID AI DGVDVI+LSLG++ VPLYEDP+A+AT+AAM
Sbjct: 254 PRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVIALSLGIDRVPLYEDPVAIATFAAM 313

Query: 301 ERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLN 360
           ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTITL NGVSVLGSSLFPLN
Sbjct: 314 ERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLGSSLFPLN 373

Query: 361 SAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN 420
           SA+ +SPLPI +MG+CHN KKLKRVG+KIVVCED DE+SL LQVDNVQSAK+AVGVFISN
Sbjct: 374 SAIALSPLPIFFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQSAKIAVGVFISN 433

Query: 421 ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 480
           ISDWDNLIQT FPSIFL+ Y GN++KDYI  SS+PKARVNFHKTI+GTKPAPSVARYSSR
Sbjct: 434 ISDWDNLIQTSFPSIFLNAYHGNVLKDYINRSSNPKARVNFHKTIIGTKPAPSVARYSSR 493

Query: 481 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 540
           GPSESCPFVLKPDIMAPGDAILASWPQNVAATDV SRPIYSKFNVLSGTSM+CPHAAGVA
Sbjct: 494 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSGTSMACPHAAGVA 553

Query: 541 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 600
           ALLKGAHP WS AAIRSAMMTTAD++DNT+T IKDLG++NK+ATPLAMGSGHVNPNKA+D
Sbjct: 554 ALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAID 613

Query: 601 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSKA 660
           PGL+YDMGI+DY NL+CALNYTKNQIQTITRSTSN+CE PSLDLNYPSFII VNASDS+ 
Sbjct: 614 PGLIYDMGIEDYTNLICALNYTKNQIQTITRSTSNDCEKPSLDLNYPSFIITVNASDSET 673

Query: 661 TR-EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGR 720
            R EISREF R VTNIGEKG TY+AK+  M GL VTV+PKKLKFK KN  LSFKLKI G 
Sbjct: 674 GRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKKLKFKRKNQNLSFKLKIRGH 733

Query: 721 ARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSLKS 755
             VKRE+DVVFGYL+WVEVGG H VQSPIVVT++ S
Sbjct: 734 VSVKRESDVVFGYLNWVEVGGGHRVQSPIVVTNMSS 767

BLAST of Lcy01g018100.1 vs. NCBI nr
Match: XP_022962414.1 (subtilisin-like protease SBT1.9 [Cucurbita moschata])

HSP 1 Score: 1238.4 bits (3203), Expect = 0.0e+00
Identity = 630/763 (82.57%), Postives = 689/763 (90.30%), Query Frame = 0

Query: 1   MAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDNSRLGTMASSSSSS 60
           M IF    T T+T+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD S L T  +SSSSS
Sbjct: 14  MVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT-TTSSSSS 73

Query: 61  SLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSN 120
           SL SKLIH YKHAINGF+ASLTPS+L+AL+ SPGYVSS+RDSSV+ DTTHSS+FL LS N
Sbjct: 74  SLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTTHSSNFLALSPN 133

Query: 121 SGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKL 180
           SGLLPISNYGSDVIIG VDTGVWPES SFNDDG+S+IPSRWKGECESG HF  SLCNKKL
Sbjct: 134 SGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNASLCNKKL 193

Query: 181 IGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 240
           IG RFFNKGLI+KFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG G ARGVA
Sbjct: 194 IGGRFFNKGLISKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVA 253

Query: 241 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAM 300
           PRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG++GVPLYEDP+A+AT+AAM
Sbjct: 254 PRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAM 313

Query: 301 ERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLN 360
           ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTITL NGVSVLGSSLFPLN
Sbjct: 314 ERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLGSSLFPLN 373

Query: 361 SAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN 420
           SA+ +SPLPIV+MG+CHN KKLKRVG+KIVVCED DE+SL LQVDNV+SAK+AVGVFISN
Sbjct: 374 SAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKIAVGVFISN 433

Query: 421 ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 480
           ISDWDNLIQT FPSIFL+ Y GN+IKDYIK SS+PKARVNFHKTI+GTKPAPSVARYSSR
Sbjct: 434 ISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAPSVARYSSR 493

Query: 481 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 540
           GPSESCPFVLKPDIMAPGDAILASWPQNVAATDV SRPIYSKFNVLSGTSM+CPHAAGVA
Sbjct: 494 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSGTSMACPHAAGVA 553

Query: 541 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 600
           ALLKGAHP WS AAIRSAMMTTAD++DNT+T IKDLG++NK+ATPLAMGSGHVNPNKA+D
Sbjct: 554 ALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAID 613

Query: 601 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSKA 660
           PGL+YDMGI+DY NLLCALNYTKNQIQTITRSTSN+CE   LDLNYPSFI+ VNASDS+ 
Sbjct: 614 PGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPSFIMTVNASDSQT 673

Query: 661 TR-EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGR 720
            R E+SREF R VTNIGEKG TY+AK+  M GL VTV+P KLKFK KN  LSFKLKI G 
Sbjct: 674 GRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLSFKLKIRGH 733

Query: 721 ARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSLKSKVKSHRN 762
             +KRE+DVVFGYL+WVEVGG H VQSPIVV ++ + ++SH N
Sbjct: 734 VSIKRESDVVFGYLNWVEVGGGHRVQSPIVV-AVVAGLRSHWN 774

BLAST of Lcy01g018100.1 vs. NCBI nr
Match: KAG6598336.1 (Subtilisin-like protease 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1236.1 bits (3197), Expect = 0.0e+00
Identity = 630/763 (82.57%), Postives = 686/763 (89.91%), Query Frame = 0

Query: 1   MAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDNSRLGTMASSSSSS 60
           + IF    T T+T+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD S L T  SSSSSS
Sbjct: 14  IVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRTTTSSSSSS 73

Query: 61  SLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSN 120
              SKLIH YKHAINGF+A+LTPS+L+AL+ SPGYVSSVRDSSV+ DTTHSS+FL LS N
Sbjct: 74  --PSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNFLALSPN 133

Query: 121 SGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKL 180
           SGLLPISNYGSDVIIG VDTGVWPES SFNDDG+ +IPSRWKGECESG HF  SLCNKKL
Sbjct: 134 SGLLPISNYGSDVIIGFVDTGVWPESESFNDDGIPKIPSRWKGECESGTHFNASLCNKKL 193

Query: 181 IGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 240
           IG RFFNKGLIAKFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG G ARGVA
Sbjct: 194 IGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVA 253

Query: 241 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAM 300
           PRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG++GVPLYEDP+A+AT+AAM
Sbjct: 254 PRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAM 313

Query: 301 ERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLN 360
           ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTITL NGVSVLGSSLFPLN
Sbjct: 314 ERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLGSSLFPLN 373

Query: 361 SAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN 420
            A+ +SPLPIV+MG+CHN KKLKRVG+KIVVCED DE+SL LQVDNV+SAK+AVGVFISN
Sbjct: 374 LAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKIAVGVFISN 433

Query: 421 ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 480
           ISDWDNLIQT FPSIFL+ Y GN+IKDYIK SS+PKARVNFHKTI+GTKPAPSVARYSSR
Sbjct: 434 ISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAPSVARYSSR 493

Query: 481 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 540
           GPSESCPFVLKPDIMAPGDAILASWPQNVAATDV SRPIYSK+NVLSGTSM+CPHAAGVA
Sbjct: 494 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKYNVLSGTSMACPHAAGVA 553

Query: 541 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 600
           ALLKGAHP WS AAIRSAMMTTAD++DNT+T IKDLG++NK+ATPLAMGSGHVNPNKA+D
Sbjct: 554 ALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAID 613

Query: 601 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSKA 660
           PGL+YDMGI+DY NLLCALNYTKNQIQTITRSTSN+CE P LDLNYPSFII VNASDS+ 
Sbjct: 614 PGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKPLLDLNYPSFIITVNASDSQT 673

Query: 661 TR-EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGR 720
            R EISREF R VTNIGEKG TY+AK+  M GL VTV+P KLKFK KN  LSFKLKI G 
Sbjct: 674 GRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLSFKLKIRGH 733

Query: 721 ARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSLKSKVKSHRN 762
             VKRE+DVVFGYL+WVEVGG H VQSPIVV ++ + ++SH N
Sbjct: 734 VSVKRESDVVFGYLNWVEVGGGHRVQSPIVV-AVVAGLRSHWN 773

BLAST of Lcy01g018100.1 vs. NCBI nr
Match: KAG7029306.1 (Subtilisin-like protease SBT1.9, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1234.6 bits (3193), Expect = 0.0e+00
Identity = 630/763 (82.57%), Postives = 685/763 (89.78%), Query Frame = 0

Query: 1   MAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDNSRLGTMASSSSSS 60
           M IF    T T+T+NYIVHMDLAAMP  FA+HHSWYSATLSSV LD S L T  SSSSSS
Sbjct: 14  MVIFLARSTLTQTDNYIVHMDLAAMPNSFATHHSWYSATLSSVLLDTSPLRTTTSSSSSS 73

Query: 61  SLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSN 120
              SKLIH YKHAINGF+A+LTPS+L+AL+ SPGYVSSVRDSSV+ DTTHSS+FL LS N
Sbjct: 74  --PSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNFLALSPN 133

Query: 121 SGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKL 180
           SGLLPISNYGSDVIIG VDTGVWPES SFNDDG+S+IPSRWKGECESG HF  SLCNKKL
Sbjct: 134 SGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFNASLCNKKL 193

Query: 181 IGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 240
           IG RFFNKGLIAKFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG G ARGVA
Sbjct: 194 IGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVA 253

Query: 241 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAM 300
           PRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG++GVPLYEDP+A+AT+AAM
Sbjct: 254 PRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAM 313

Query: 301 ERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLN 360
           ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTITL NGVSVLGSSLFPLN
Sbjct: 314 ERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVLGSSLFPLN 373

Query: 361 SAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN 420
            A+ +SPLPIV+MG+CHN KKLKRVG+KIVVCED DE+SL LQVDNV+SAK+AVGVFISN
Sbjct: 374 LAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKIAVGVFISN 433

Query: 421 ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 480
           ISDWDNLIQT FPSIFL+ Y GN+IKDYIK SS+PKARVNFHKTI+GTKPAPSVARYSSR
Sbjct: 434 ISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAPSVARYSSR 493

Query: 481 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 540
           GPSESCPFVLKPDIMAPGDAILASWPQNVAATDV SRPIYSK+NVLSGTSM+CPHAAGVA
Sbjct: 494 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKYNVLSGTSMACPHAAGVA 553

Query: 541 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 600
           ALLKGAHP WS AAIRSAMMTTAD++DNT+T IKDLG++NK+ATPLAMGSGHVNPNKA+D
Sbjct: 554 ALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAID 613

Query: 601 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSKA 660
           PGL+YDMGI+DY NLLCALNYTKNQIQTITRSTSN+CE P LDLNYPSFII VNASDS+ 
Sbjct: 614 PGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKPLLDLNYPSFIITVNASDSQT 673

Query: 661 TR-EISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGR 720
            R EISREF R VTNIGEKG TY+AK+  M GL VTV+P KLKFK KN  LSFKLKI G 
Sbjct: 674 GRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLSFKLKIRGH 733

Query: 721 ARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSLKSKVKSHRN 762
             VKRE+DVVFGYL+WVEVGG H VQSPIVV ++ + ++SH N
Sbjct: 734 VSVKRESDVVFGYLNWVEVGGGHRVQSPIVV-AVVAGLRSHWN 773

BLAST of Lcy01g018100.1 vs. NCBI nr
Match: XP_022996869.1 (subtilisin-like protease SBT1.9 [Cucurbita maxima])

HSP 1 Score: 1233.0 bits (3189), Expect = 0.0e+00
Identity = 628/756 (83.07%), Postives = 681/756 (90.08%), Query Frame = 0

Query: 1   MAIFWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSV-LDNSRLGTMASSSSSS 60
           M I     T TET+NYIVHMDLAAMPKPFA+HHSWYSATLSSV LD S L T  ++SSSS
Sbjct: 14  MVILLARSTLTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT--TTSSSS 73

Query: 61  SLSSKLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSN 120
           SL SKLIH YKHAINGF+A+LTPS+L+AL+ SPGYVSSVRDSSV+ DTTHSS+FL L+ N
Sbjct: 74  SLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSSNFLALTPN 133

Query: 121 SGLLPISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKL 180
           SGLLPISNYGSDVIIG VDTGVWPES SFND+ +S+IPSRWKGECESG HF  SLCNKKL
Sbjct: 134 SGLLPISNYGSDVIIGFVDTGVWPESESFNDEAISKIPSRWKGECESGTHFNASLCNKKL 193

Query: 181 IGARFFNKGLIAKFPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 240
           IG RFFNKGLIAKFPN+TISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG G ARGVA
Sbjct: 194 IGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGQGTARGVA 253

Query: 241 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAM 300
           PRARVAIYKA+W EGN VSDVIAAID AI DGVDVISLSLG++GVPLYEDP+A+AT+AAM
Sbjct: 254 PRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAM 313

Query: 301 ERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLN 360
           ERGIFVATSAGN GPQ GTVH+GAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLF LN
Sbjct: 314 ERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFVLN 373

Query: 361 SAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN 420
           SA+ +SPLPIV+MG+CHN KKLKRVG+KIVVCED DE+SL LQVDNVQSAK+AVGVFISN
Sbjct: 374 SAIVLSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQSAKIAVGVFISN 433

Query: 421 ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 480
           ISDWDNLIQT FPSIFL+ Y GN+IKDYI  SS+PKARVNFHKTI+GTKPAPSVARYSSR
Sbjct: 434 ISDWDNLIQTSFPSIFLNAYHGNVIKDYIMRSSNPKARVNFHKTIIGTKPAPSVARYSSR 493

Query: 481 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 540
           GPSESCP VLKPDIMAPGDAILASWPQ VAATDV SRPIYSKFNVLSGTSM+CPHAAGVA
Sbjct: 494 GPSESCPLVLKPDIMAPGDAILASWPQKVAATDVYSRPIYSKFNVLSGTSMACPHAAGVA 553

Query: 541 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 600
           ALLKGAHP WS AAIRSAMMTTADV+DNT+T IKDLG++NK+ATPLAMGSGHVNPNKA+D
Sbjct: 554 ALLKGAHPGWSPAAIRSAMMTTADVVDNTQTLIKDLGNKNKVATPLAMGSGHVNPNKAID 613

Query: 601 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLDLNYPSFIIIVNASDSKA 660
           PGL+YD+GI+DY NLLCALNYTKNQIQTITRS SN+CE PSLDLNYPSFII VNASDS++
Sbjct: 614 PGLIYDIGIEDYTNLLCALNYTKNQIQTITRSPSNDCEKPSLDLNYPSFIITVNASDSES 673

Query: 661 -TREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLSFKLKIEGR 720
             REISREF R VTNIGEKG TY+AK+  M GL VTV+PK LKFK KN  LSFKLKI G 
Sbjct: 674 GRREISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKMLKFKRKNQNLSFKLKIRGH 733

Query: 721 ARVKRETDVVFGYLSWVEVGGKHIVQSPIVVTSLKS 755
             VKRE+DVVFGYL+WVEVGG H VQSPIVV  L+S
Sbjct: 734 VSVKRESDVVFGYLNWVEVGGGHRVQSPIVVAGLRS 767

BLAST of Lcy01g018100.1 vs. TAIR 10
Match: AT5G67090.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 593.6 bits (1529), Expect = 2.3e-169
Identity = 337/766 (43.99%), Postives = 471/766 (61.49%), Query Frame = 0

Query: 4   FWLPFTFTETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSS 63
           F++     ET  YI+HMDL+A P PF+ H SW+S TL+SV+ N +               
Sbjct: 13  FFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVITNRK--------------P 72

Query: 64  KLIHSYKHAINGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSGLL 123
           K+I++Y  +++GFSA LT SEL+ L+  PGYVS  +D  V++ TT S  F+GL+S SG  
Sbjct: 73  KIIYAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNSTSGTW 132

Query: 124 PISNYGSDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGAR 183
           P+SNYG+ ++IG++DTG+WP+S SF+DDG+  +PS+WKG CE  +    SLCNKKLIGA+
Sbjct: 133 PVSNYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFNS---SSLCNKKLIGAK 192

Query: 184 FFNKGLIAKFPNITIS----MNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVA 243
            FNKGL A  P++  +     +S  DT GHGTH +  AAG++VK AS+F Y  G A G+A
Sbjct: 193 VFNKGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTASGIA 252

Query: 244 PRARVAIYKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGV--------NGVPLYEDPI 303
           P A +AIYKA W EG Y SDVIAAID AI DGV VISLSLG+        +G  L  DPI
Sbjct: 253 PHAHLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLENDPI 312

Query: 304 AVATYAAMERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVL 363
           AVA++AA+++G+FV TS GN+GP   ++ NGAPW++ V AGT+ R F GT+T  N VS  
Sbjct: 313 AVASFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNRVSFS 372

Query: 364 GSSLFPLNSAMTVSPLPIVYMGRCHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKV 423
             SLFP        P+  +  G   N    K +  +IVVC   +  ++  ++  ++S   
Sbjct: 373 FPSLFPGEFPSVQFPVTYIESGSVEN----KTLANRIVVC--NENINIGSKLHQIRSTGA 432

Query: 424 AVGVFISN-ISDWDNLIQTPFPSIFLSPYRGNIIKDYIKGS-SDPKARVNFHKTILGTKP 483
           A  V I++ + +  + I+  FP  F+       I+ Y   + ++  A++ F KT++GTKP
Sbjct: 433 AAVVLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEFRKTVIGTKP 492

Query: 484 APSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTS 543
           AP V  YSSRGP  S P +LKPDI+APG  IL++WP     T   + P++S FN+L+GTS
Sbjct: 493 APEVGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFSGFNLLTGTS 552

Query: 544 MSCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGS 603
           M+ PH AGVAAL+K  HP WS +AI+SA+MTTA  LDN                PLA+G+
Sbjct: 553 MAAPHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN----------------PLAVGA 612

Query: 604 GHVNPNKAMDPGLVYDMGIQDYVNLLC-ALNYTKNQIQTITRST-SNNCENPSLDLNYPS 663
           GHV+ NK ++PGL+YD   QD++N LC     ++  I  ITRS  S+ C+ PS  LNYPS
Sbjct: 613 GHVSTNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKKPSPYLNYPS 672

Query: 664 FIIIVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKND 723
            I    +  S       + F R +TN+GE   +Y  ++  + GL V V+PKKL F  KN+
Sbjct: 673 IIAYFTSDQSS-----PKIFKRTLTNVGEAKRSYIVRVRGLKGLNVVVEPKKLMFSEKNE 732

Query: 724 KLSFKLKIEGRARVKRETDVVFGYLSWV-EVGGKHIVQSPIVVTSL 753
           KLS+ +++E    +  + +VV+G +SWV E   +  V   +V TSL
Sbjct: 733 KLSYTVRLESPRGL--QENVVYGLVSWVDEDEAEFEVSCSVVATSL 732

BLAST of Lcy01g018100.1 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 545.0 bits (1403), Expect = 9.3e-155
Identity = 315/757 (41.61%), Postives = 443/757 (58.52%), Query Frame = 0

Query: 11  TETENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSSKLIHSYK 70
           T  + YI+ ++ +  P+ F +HH WY++ L+S                    S L+++Y 
Sbjct: 25  TAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS-------------------ESSLLYTYT 84

Query: 71  HAINGFSASLTPSELEALQKSPGYVSSV-RDSSVQVDTTHSSHFLGLSSNSGLLPISNYG 130
            + +GFSA L  +E ++L  S   +  +  D    + TT +  FLGL+S  G+  + +  
Sbjct: 85  TSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSS 144

Query: 131 SDVIIGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGARFFNKGL 190
           + VIIGV+DTGVWPES SF+D  M EIPS+WKGECESG+ F   LCNKKLIGAR F+KG 
Sbjct: 145 NGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGF 204

Query: 191 -IAKFPNITISMNST--RDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRARVAI 250
            +A     +    S   RD +GHGTHTSTTAAGS V+ ASF GY +G ARG+A RARVA 
Sbjct: 205 QMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVAT 264

Query: 251 YKAVWAEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAMERGIFVA 310
           YK  W+ G + SD++AA+D AILDGVDV+SLSLG    P Y D IA+  ++AMERG+FV+
Sbjct: 265 YKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVS 324

Query: 311 TSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLNSAMTVSP 370
            SAGN+GP   +V N APWV+ V AGT+DRDF     L NG  + G SL+     M   P
Sbjct: 325 CSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS-GVGMGTKP 384

Query: 371 LPIVYMGRCHNSKKLKRVGY--------KIVVCEDKDEHSLSLQVDNVQSAKVAVG---V 430
           L +VY     +S  L   G         KIVVC    +  ++ +V+     + A G   +
Sbjct: 385 LELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVC----DRGVNARVEKGAVVRDAGGLGMI 444

Query: 431 FISNISDWDNLIQTP--FPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSV 490
             +  +  + L+      P+I +    G+++++Y+K  S P A + F  T+L  KP+P V
Sbjct: 445 MANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVV 504

Query: 491 ARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCP 550
           A +SSRGP+   P +LKPD++ PG  ILA W   +  T ++     ++FN++SGTSMSCP
Sbjct: 505 AAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCP 564

Query: 551 HAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVN 610
           H +G+A LLK AHP WS +AI+SA+MTTA VLDNT  P+ D  D N L+ P A GSGHV+
Sbjct: 565 HISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAAD-NSLSNPYAHGSGHVD 624

Query: 611 PNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSLD---LNYPSFII 670
           P KA+ PGLVYD+  ++Y+  LC+L+YT + I  I +  S NC     D   LNYPSF +
Sbjct: 625 PQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSV 684

Query: 671 IVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDKLS 730
           +         + + R +TR VTN+G   + YK  +     + ++VKP KL FK   +K  
Sbjct: 685 LFGG------KRVVR-YTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKR 744

Query: 731 FKLKIEGRARVKRETDVVFGYLSWVEVGGKHIVQSPI 748
           + +    +  V       FG ++W     +H V+SP+
Sbjct: 745 YTVTFVSKKGVSMTNKAEFGSITW--SNPQHEVRSPV 747

BLAST of Lcy01g018100.1 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 526.9 bits (1356), Expect = 2.6e-149
Identity = 314/757 (41.48%), Postives = 449/757 (59.31%), Query Frame = 0

Query: 16  YIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSSKLIHSYKHAING 75
           YIVHM  + MP  F  H +WY ++L S+ D                S++L+++Y++AI+G
Sbjct: 32  YIVHMAKSQMPSSFDLHSNWYDSSLRSISD----------------SAELLYTYENAIHG 91

Query: 76  FSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSG-LLPISNYGSDVII 135
           FS  LT  E ++L   PG +S + +   ++ TT +  FLGL  ++  L P +   SDV++
Sbjct: 92  FSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVV 151

Query: 136 GVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGARFFNKGLIAKFP 195
           GV+DTGVWPES S++D+G   IPS WKG CE+G +F  SLCN+KLIGARFF +G  +   
Sbjct: 152 GVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMG 211

Query: 196 NITIS--MNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRARVAIYKAVWA 255
            I  S    S RD +GHGTHTS+TAAGS V+ AS  GY SG ARG+APRARVA+YK  W 
Sbjct: 212 PIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWL 271

Query: 256 EGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAMERGIFVATSAGNN 315
            G + SD++AAID AI D V+V+S+SLG      Y D +A+  +AAMERGI V+ SAGN 
Sbjct: 272 GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNA 331

Query: 316 GPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLNSAMTVSPLPIVYM 375
           GP   ++ N APW+  V AGT+DRDF     L NG +  G SLF    A+    LP +Y 
Sbjct: 332 GPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK-GEALPDKLLPFIYA 391

Query: 376 GRCHNSKK----------LKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN-IS 435
           G   N+             ++V  KIV+C+     +  +Q  +V  A   VG+ ++N  +
Sbjct: 392 GNASNATNGNLCMTGTLIPEKVKGKIVMCD--RGINARVQKGDVVKAAGGVGMILANTAA 451

Query: 436 DWDNLIQTP--FPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAPSVARYSSR 495
           + + L+      P+  +    G+II+ Y+    +P A ++   T++G KP+P VA +SSR
Sbjct: 452 NGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSR 511

Query: 496 GPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMSCPHAAGVA 555
           GP+   P +LKPD++APG  ILA+W      T + S     +FN++SGTSMSCPH +G+A
Sbjct: 512 GPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLA 571

Query: 556 ALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGHVNPNKAMD 615
           ALLK  HP WS AAIRSA+MTTA        P+ D+    K +TP   G+GHV+P  A +
Sbjct: 572 ALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIA-TGKPSTPFDHGAGHVSPTTATN 631

Query: 616 PGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPS-----LDLNYPSFIIIVNA 675
           PGL+YD+  +DY+  LCALNYT  QI++++R   N   +PS      DLNYPSF + V+ 
Sbjct: 632 PGLIYDLTTEDYLGFLCALNYTSPQIRSVSR--RNYTCDPSKSYSVADLNYPSFAVNVDG 691

Query: 676 SDSKATREISREFTRMVTNIGEKGTTYKAKLIA-MDGLRVTVKPKKLKFKWKNDKLSFKL 735
                    + ++TR VT++G  G TY  K+ +   G++++V+P  L FK  N+K S+ +
Sbjct: 692 VG-------AYKYTRTVTSVGGAG-TYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTV 751

Query: 736 KIEGRARVKRETDVVFGYLSWVEVGGKHIVQSPIVVT 751
                +  K      FG + W +  GKH+V SP+ ++
Sbjct: 752 TFTVDSS-KPSGSNSFGSIEWSD--GKHVVGSPVAIS 755

BLAST of Lcy01g018100.1 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 523.1 bits (1346), Expect = 3.8e-148
Identity = 319/766 (41.64%), Postives = 443/766 (57.83%), Query Frame = 0

Query: 14  ENYIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSSKLIHSYKHAI 73
           + +I  +D  +MP  F +H+ WYS   +                     S+++H Y    
Sbjct: 26  KTFIFRIDGGSMPSIFPTHYHWYSTEFAE-------------------ESRIVHVYHTVF 85

Query: 74  NGFSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSSNSGLLPISNYGSDVI 133
           +GFSA +TP E + L+  P  ++   D   ++ TT S  FLGL +  GL   S+YGSDVI
Sbjct: 86  HGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDVI 145

Query: 134 IGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGARFFNKGLIAKF 193
           IGV DTG+WPE  SF+D  +  IP RW+G CESGA F    CN+K+IGARFF KG  A  
Sbjct: 146 IGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAV 205

Query: 194 ---PNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRARVAIYKAV 253
               N T+   S RD +GHGTHTS+TAAG +  +AS  GY SG+A+GVAP+AR+A YK  
Sbjct: 206 IGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVC 265

Query: 254 WAE-GNYVSDVIAAIDHAILDGVDVISLSL-GVNGV--PLYEDPIAVATYAAMERGIFVA 313
           W + G   SD++AA D A+ DGVDVIS+S+ G +G+  P Y DPIA+ +Y A  +GIFV+
Sbjct: 266 WKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVS 325

Query: 314 TSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLF---PLNSAMT 373
           +SAGN GP   +V N APWV  V A T+DR+F     L +G  + G SL+   PLN  M 
Sbjct: 326 SSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNGRM- 385

Query: 374 VSPLPIVYMGR---------CHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVG 433
               P+VY G+           N+   K+V  KIV+C+      ++  +  V      VG
Sbjct: 386 ---FPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGL--VVKKAGGVG 445

Query: 434 VFISN-ISDWDNLIQTP--FPSIFLSPYRGNIIKDYIKGSSDPKARVNFHKTILGTKPAP 493
           + ++N  S+ + L+      P+  +    G+ IK Y     +P A ++F  TI+G KPAP
Sbjct: 446 MILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAP 505

Query: 494 SVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSMS 553
            +A +S RGP+   P +LKPD++APG  ILA+W   V  T + S P  ++FN+LSGTSM+
Sbjct: 506 VIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMA 565

Query: 554 CPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSGH 613
           CPH +G AALLK AHP WS A IRSAMMTT +++DN+   + D     K ATP   GSGH
Sbjct: 566 CPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLID-ESTGKSATPYDYGSGH 625

Query: 614 VNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSN--NCENPSL-DLNYPSF 673
           +N  +AM+PGLVYD+   DY+  LC++ Y    IQ ITR+         PS  +LNYPS 
Sbjct: 626 LNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPS- 685

Query: 674 IIIVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWKNDK 733
             I     +     +S+   R  TN+G+    Y+A++ +  G+ VTVKP +L F     +
Sbjct: 686 --ITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKR 745

Query: 734 LSFKLKIEGRAR--VKRETDVVFGYLSWVEVGGKHIVQSPIVVTSL 753
            S+ + +    R  V  ET  VFG ++W + GGKH+V+SPIVVT +
Sbjct: 746 RSYAVTVTVNTRNVVLGETGAVFGSVTWFD-GGKHVVRSPIVVTQM 761

BLAST of Lcy01g018100.1 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 517.3 bits (1331), Expect = 2.1e-146
Identity = 319/768 (41.54%), Postives = 446/768 (58.07%), Query Frame = 0

Query: 16  YIVHMDLAAMPKPFASHHSWYSATLSSVLDNSRLGTMASSSSSSSLSSKLIHSYKHAING 75
           YIVH+D  A P  F +H  WY+++L+S+               +S    +IH+Y    +G
Sbjct: 28  YIVHVDHEAKPSIFPTHFHWYTSSLASL---------------TSSPPSIIHTYDTVFHG 87

Query: 76  FSASLTPSELEALQKSPGYVSSVRDSSVQVDTTHSSHFLGLSS--NSGLLPISNYGSDVI 135
           FSA LT  +   L   P  +S + +    + TT S  FLGL S   +GLL  S++GSD++
Sbjct: 88  FSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLV 147

Query: 136 IGVVDTGVWPESGSFNDDGMSEIPSRWKGECESGAHFIGSLCNKKLIGARFFNKGLIAK- 195
           IGV+DTGVWPE  SF+D G+  +P +WKG+C +   F  S CN+KL+GARFF  G  A  
Sbjct: 148 IGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATN 207

Query: 196 -FPNITISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYGSGIARGVAPRARVAIYKAVW 255
              N T    S RD++GHGTHT++ +AG YV  AS  GY  G+A G+AP+AR+A YK  W
Sbjct: 208 GKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCW 267

Query: 256 AEGNYVSDVIAAIDHAILDGVDVISLSLGVNGVPLYEDPIAVATYAAMERGIFVATSAGN 315
             G Y SD++AA D A+ DGVDVISLS+G   VP Y D IA+  + A++RGIFV+ SAGN
Sbjct: 268 NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGN 327

Query: 316 NGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLNNGVSVLGSSLFPLNSAMTVSPLPIVY 375
            GP   TV N APW+  V AGT+DRDF   + L NG  + G S++           P+VY
Sbjct: 328 GGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVY 387

Query: 376 MGR------------CHNSKKLKRVGYKIVVCEDKDEHSLSLQVDNVQSAKVAVGVFISN 435
            G                S     V  KIV+C D+  +S + + + V+     +G+ I+N
Sbjct: 388 GGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLC-DRGINSRATKGEIVRK-NGGLGMIIAN 447

Query: 436 -ISDWDNLIQT--PFPSIFLSPYRGNIIKDYI------KGSSDPKARVNFHKTILGTKPA 495
            + D + L+      P+  +    G+ I+ YI      + S  P A + F  T LG +PA
Sbjct: 448 GVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPA 507

Query: 496 PSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVNSRPIYSKFNVLSGTSM 555
           P VA +S+RGP+   P +LKPD++APG  ILA+WP  +  + V S    ++FN+LSGTSM
Sbjct: 508 PVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSM 567

Query: 556 SCPHAAGVAALLKGAHPRWSLAAIRSAMMTTADVLDNTRTPIKDLGDRNKLATPLAMGSG 615
           +CPH +G+AALLK AHP WS AAIRSA++TTA  +DN+  P+ D    N  ++ +  GSG
Sbjct: 568 ACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGN-TSSVMDYGSG 627

Query: 616 HVNPNKAMDPGLVYDMGIQDYVNLLCALNYTKNQIQTITRSTSNNCENPSL-----DLNY 675
           HV+P KAMDPGLVYD+   DY+N LC  NYT+  I TITR  + +C+         +LNY
Sbjct: 628 HVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA-DCDGARRAGHVGNLNY 687

Query: 676 PSFIIIVNASDSKATREISREFTRMVTNIGEKGTTYKAKLIAMDGLRVTVKPKKLKFKWK 735
           PSF ++          ++S  F R VTN+G+  + Y+ K+    G  VTV+P+KL F+  
Sbjct: 688 PSFSVVF---QQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRV 747

Query: 736 NDKLSFKLKIEGRARVKRE---TDVVFGYLSWVEVGGKHIVQSPIVVT 751
             KLSF ++++    VK     T+V  G++ W +  GK  V SP+VVT
Sbjct: 748 GQKLSFVVRVK-TTEVKLSPGATNVETGHIVWSD--GKRNVTSPLVVT 770