Lag0042190.1 (mRNA) Sponge gourd (AG‐4) v1

BLAST of Lag0042190.1 vs. NCBI nr
Match: XP_008460558.1 (PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Cucumis melo] >KAA0045465.1 putative pectinesterase/pectinesterase inhibitor 45 [Cucumis melo var. makuwa] >TYK00244.1 putative pectinesterase/pectinesterase inhibitor 45 [Cucumis melo var. makuwa])

HSP 1 Score: 1030.8 bits (2664), Expect = 4.7e-297
Identity = 518/598 (86.62%), Postives = 555/598 (92.81%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVISVSVILLVVVAAVFVQVNSNSSKSSSKDSD 60
           MVFQDFDQISERRRAERARKFR+RVTIAV+S SVILLVV AAVFVQV S+SSKS++K+ D
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLVVAAAVFVQV-SSSSKSTTKNGD 60

Query: 61  -----GNSKDPDTPETKQVSRVDKMIVMICNSTDYKGKCETTLKDGVKTDPDSSDPKDLI 120
                GNSK P  PETKQVSRV+KMI MICNSTDYKGKCE+TLKDG+ TDP+SSDPKDLI
Sbjct: 61  SGSSTGNSKKPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGLHTDPNSSDPKDLI 120

Query: 121 KLAISAAATELKSAVKKAEGFNFATAEEKGAFEDCKVLFEDAVEELEISVSEMSRKNLGK 180
           KLAISAAA E+KSAVKKA GFNFAT EEKGAFEDCKVL EDAVEELE+S+SE+S+KN+GK
Sbjct: 121 KLAISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGK 180

Query: 181 LTAKTTPDLNNWLSAVISYQETCVDGFPDGKLKSDMEKAVKSVKELTSNSLAMISQVVSF 240
           LTAKTTPDLNNWLSAV+SY ETCVDGFP+GK+KS+MEK VK+ KELTSNSLAMISQV SF
Sbjct: 181 LTAKTTPDLNNWLSAVMSYHETCVDGFPEGKMKSEMEKVVKAGKELTSNSLAMISQVASF 240

Query: 241 FSTFEKPDGEEAATRRRLLARDGLPIWMNTQERRMLKGATAGDKPKPNVVVAKDGSGNFK 300
           FSTFE P+G  AA+RRRL+A  G+P WMN+ ERRMLKGA AG+KPKPNVVVAKDGSG FK
Sbjct: 241 FSTFEMPEG--AASRRRLMATKGVPTWMNSNERRMLKGAAAGEKPKPNVVVAKDGSGEFK 300

Query: 301 TINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGKKNFVDG 360
           TINEALAAMPAKYDGRYVIYVKEGIY+ETVVITKKM NVTMYGDGSQKSMISG KNFVDG
Sbjct: 301 TINEALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFVDG 360

Query: 361 VRTFQTATFVALGDGFLGQAIGFRNTAGPEKHQAVAARIQGDRAIFVNCRFDGYQDTLYA 420
           VRTFQTATFVALG+GFLGQAIGFRN AGPEKHQAVAAR+Q DRAIFVNCRF+GYQDTLYA
Sbjct: 361 VRTFQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYA 420

Query: 421 QAHRQYYRSCLITGTIDFIFGDAAAIFQNCNLMVRKPLDKQQNIVTAQGRTDKHETTGIV 480
           QAHRQ+YRSCLITGTIDFIFGDAAAIFQNCN+M+RKPLD QQNIVTAQGRTDKHETTGIV
Sbjct: 421 QAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNIVTAQGRTDKHETTGIV 480

Query: 481 LQNCKILPDKTLEPVKANFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKT 540
           LQNCKILPDKTLE VK+ FKSYLGRPWKEFSRTIVMES IED+IHPDGWM W+GDFALKT
Sbjct: 481 LQNCKILPDKTLEAVKSQFKSYLGRPWKEFSRTIVMESKIEDVIHPDGWMAWQGDFALKT 540

Query: 541 LYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESASVPVHAGLF 594
           LYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIES S PVH GLF
Sbjct: 541 LYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHVGLF 595

BLAST of Lag0042190.1 vs. NCBI nr
Match: XP_038893171.1 (putative pectinesterase/pectinesterase inhibitor 45 [Benincasa hispida])

HSP 1 Score: 1024.6 bits (2648), Expect = 3.3e-295
Identity = 513/599 (85.64%), Postives = 550/599 (91.82%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVISVSVILLVVVAAVFVQVNSNS------SKS 60
           MVFQDFDQISERRRAERARKFR+RVTIAV+SVSVILLV+ AAVFVQV+S+S        S
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSVSVILLVIAAAVFVQVSSSSKSTTKNGNS 60

Query: 61  SSKDSDGNSKDPDTPETKQVSRVDKMIVMICNSTDYKGKCETTLKDGVKTDPDSSDPKDL 120
            S  S+ NSK+    + KQVSRV+KMI MICNSTDYKGKCE+TLKDG+ TD +SSDPKDL
Sbjct: 61  KSSSSNENSKETVPDDVKQVSRVEKMITMICNSTDYKGKCESTLKDGIHTDSNSSDPKDL 120

Query: 121 IKLAISAAATELKSAVKKAEGFNFATAEEKGAFEDCKVLFEDAVEELEISVSEMSRKNLG 180
           IKL+ISAAA E+KSAVKKA GFNFATAEEKGAFEDCKVL EDAVEELE+S+S++S KN+G
Sbjct: 121 IKLSISAAADEVKSAVKKATGFNFATAEEKGAFEDCKVLLEDAVEELEMSMSQVSIKNMG 180

Query: 181 KLTAKTTPDLNNWLSAVISYQETCVDGFPDGKLKSDMEKAVKSVKELTSNSLAMISQVVS 240
           KLTAKTTPDLNNWLSAV+SY ETCVDGFP+GK+K++MEK VK+ KELTSNSLAMISQV S
Sbjct: 181 KLTAKTTPDLNNWLSAVMSYHETCVDGFPEGKIKTEMEKVVKNGKELTSNSLAMISQVAS 240

Query: 241 FFSTFEKPDGEEAATRRRLLARDGLPIWMNTQERRMLKGATAGDKPKPNVVVAKDGSGNF 300
           FFSTFE P+G  AATRRRL+A +G P WMN+ ERRMLKGATAGDKPKPNVVVAKDGSG+F
Sbjct: 241 FFSTFEMPEG--AATRRRLMASNGFPTWMNSNERRMLKGATAGDKPKPNVVVAKDGSGDF 300

Query: 301 KTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGKKNFVD 360
           KTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKM NVTMYGDGSQKSMISG KNFVD
Sbjct: 301 KTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMVNVTMYGDGSQKSMISGNKNFVD 360

Query: 361 GVRTFQTATFVALGDGFLGQAIGFRNTAGPEKHQAVAARIQGDRAIFVNCRFDGYQDTLY 420
           GVRTFQTATFVALG+GF  QAIGFRNTAGPEKHQAVAAR+Q DRAIF+NCRF+GYQDTLY
Sbjct: 361 GVRTFQTATFVALGEGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFLNCRFEGYQDTLY 420

Query: 421 AQAHRQYYRSCLITGTIDFIFGDAAAIFQNCNLMVRKPLDKQQNIVTAQGRTDKHETTGI 480
            QAHRQ+YRSCLITGTIDFIFGDAAA FQNCN+MVRKPLD QQNIVTAQGRTDKHETTGI
Sbjct: 421 VQAHRQFYRSCLITGTIDFIFGDAAAFFQNCNMMVRKPLDNQQNIVTAQGRTDKHETTGI 480

Query: 481 VLQNCKILPDKTLEPVKANFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALK 540
           VLQNCKILPDKTLEPVK+ FKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALK
Sbjct: 481 VLQNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALK 540

Query: 541 TLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESASVPVHAGLF 594
           TLYYAEFNNQGPGAKTD RVKWPGYKVIDKDEAAKFTIGTFLELDWIES S PVH GLF
Sbjct: 541 TLYYAEFNNQGPGAKTDGRVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHVGLF 597

BLAST of Lag0042190.1 vs. NCBI nr
Match: XP_004142361.1 (putative pectinesterase/pectinesterase inhibitor 45 [Cucumis sativus] >KGN52371.1 hypothetical protein Csa_008584 [Cucumis sativus])

HSP 1 Score: 1023.5 bits (2645), Expect = 7.5e-295
Identity = 509/596 (85.40%), Postives = 551/596 (92.45%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVISVSVILLVVVAAVFVQVNSNSSKSSSKD-- 60
           MVFQDFDQISERRRAERARKFR+RV IAV+S SVILLVV AAVFVQV+S+ S + + D  
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVIIAVVSASVILLVVAAAVFVQVSSSKSTTKNDDSG 60

Query: 61  -SDGNSKDPDTPETKQVSRVDKMIVMICNSTDYKGKCETTLKDGVKTDPDSSDPKDLIKL 120
            S GNSK P  PETKQVSRV+KMI MICNSTDYKGKCE+TLKDGV TDP+SSDPKDLIKL
Sbjct: 61  SSSGNSKQPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGVHTDPNSSDPKDLIKL 120

Query: 121 AISAAATELKSAVKKAEGFNFATAEEKGAFEDCKVLFEDAVEELEISVSEMSRKNLGKLT 180
           AISAAA E+KSAVKKA GFNFAT EEKGAFEDCKVL EDA+EELE+S+SE+++KN+GKLT
Sbjct: 121 AISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAIEELEMSMSEVNKKNMGKLT 180

Query: 181 AKTTPDLNNWLSAVISYQETCVDGFPDGKLKSDMEKAVKSVKELTSNSLAMISQVVSFFS 240
           AKTTP+LNNWLSAV+SY ETCVDGFP+GK+KSD+EK VK+ KELTSNSLAMISQV SFFS
Sbjct: 181 AKTTPNLNNWLSAVMSYHETCVDGFPEGKMKSDIEKVVKAGKELTSNSLAMISQVASFFS 240

Query: 241 TFEKPDGEEAATRRRLLARDGLPIWMNTQERRMLKGATAGDKPKPNVVVAKDGSGNFKTI 300
           TFE P+G  AA+RRRL+  +G+P WM+  +RRMLKGA AG+KPKPNVVVAKDGSG FKTI
Sbjct: 241 TFEMPEG--AASRRRLMTTNGVPTWMDRNQRRMLKGAAAGEKPKPNVVVAKDGSGEFKTI 300

Query: 301 NEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGKKNFVDGVR 360
           NEALAAMPAKYDGRYVIYVKEGIY+ETVVITKKM NVTMYGDGSQKSMISG KNFVDGVR
Sbjct: 301 NEALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFVDGVR 360

Query: 361 TFQTATFVALGDGFLGQAIGFRNTAGPEKHQAVAARIQGDRAIFVNCRFDGYQDTLYAQA 420
           TFQTATFVALG+GFLGQAIGFRN AGPEKHQAVAAR+Q DRAIFVNCRF+GYQDTLY QA
Sbjct: 361 TFQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYTQA 420

Query: 421 HRQYYRSCLITGTIDFIFGDAAAIFQNCNLMVRKPLDKQQNIVTAQGRTDKHETTGIVLQ 480
           HRQ+YRSCLITGTIDFIFGDAAAIFQNCN+M+RKPLD QQN+VTAQGRTDKHETTGIVLQ
Sbjct: 421 HRQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNMVTAQGRTDKHETTGIVLQ 480

Query: 481 NCKILPDKTLEPVKANFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLY 540
           NCKILPDKTLEPVK+ FKSYLGRPWKEFSRTIVMESTIED+IHPDGWM WEGDFALKTLY
Sbjct: 481 NCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDVIHPDGWMAWEGDFALKTLY 540

Query: 541 YAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESASVPVHAGLF 594
           YAEFNN+GPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIES S PVH GLF
Sbjct: 541 YAEFNNKGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHVGLF 594

BLAST of Lag0042190.1 vs. NCBI nr
Match: XP_022971606.1 (putative pectinesterase/pectinesterase inhibitor 45 [Cucurbita maxima])

HSP 1 Score: 947.6 bits (2448), Expect = 5.2e-272
Identity = 476/603 (78.94%), Postives = 526/603 (87.23%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVISVSVILLVVVAAVFVQVN----------SN 60
           MVFQDFDQISERRRAERARKFR RV IAV+S  +IL+ VVAAVFVQV+          + 
Sbjct: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVFVVAAVFVQVSPPTKSTTRDGAG 60

Query: 61  SSKSSSKDSDGNSKDPDTPETKQVSRVDKMIVMICNSTDYKGKCETTLKDGVKTDPDSSD 120
           +  SSS  S G+SKD  T + KQVSR  KMI MICNSTDYKGKCE+TLK GV+T PDSSD
Sbjct: 61  AGSSSSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKGKCESTLKQGVETHPDSSD 120

Query: 121 PKDLIKLAISAAATELKSAVKKAEGFNFATAEEKGAFEDCKVLFEDAVEELEISVSEMSR 180
           PKDLIKLAI+A +TE+ SA+KKA  FNF T EEKGAFEDCK+L E+AVEEL+ SVSE+SR
Sbjct: 121 PKDLIKLAIAAVSTEVNSALKKASAFNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSR 180

Query: 181 KNLGKLTAKTTPDLNNWLSAVISYQETCVDGFPDGKLKSDMEKAVKSVKELTSNSLAMIS 240
           K+LGKLT KTTPDLNNWLSAV+SY  TCVDGFP+GK K+ ME  +K+ KELTSNSLAMIS
Sbjct: 181 KDLGKLTDKTTPDLNNWLSAVMSYHATCVDGFPEGKSKTVMEATLKAGKELTSNSLAMIS 240

Query: 241 QVVSFFSTFEKPDGEEAATRRRLLARDGLPIWMNTQERRMLKGATAGDKPKPNVVVAKDG 300
           QV +FFSTFEK DG  AATRRRL+A+DGLP WM + ERRMLKG  A DKP PNVVVAKDG
Sbjct: 241 QVATFFSTFEKSDGGGAATRRRLMAKDGLPSWMTSNERRMLKGDAADDKPTPNVVVAKDG 300

Query: 301 SGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGKK 360
           SGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISG K
Sbjct: 301 SGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGNK 360

Query: 361 NFVDGVRTFQTATFVALGDGFLGQAIGFRNTAGPEKHQAVAARIQGDRAIFVNCRFDGYQ 420
           N  DGVR FQTA+FVALGDG L + IGFRNTAGPEK QAVAAR+QGDRAIFVNCRF+G+Q
Sbjct: 361 NSADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGFQ 420

Query: 421 DTLYAQAHRQYYRSCLITGTIDFIFGDAAAIFQNCNLMVRKPLDKQQNIVTAQGRTDKHE 480
           DTLYAQAHRQ+YRSCLITGT+D IFGDAAAIFQNCN M++KPLDKQ N+VTAQGR+D+HE
Sbjct: 421 DTLYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRHE 480

Query: 481 TTGIVLQNCKILPDKTLEPVKANFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGD 540
           TT  VLQNCKILPDKTLE VKA FKSYLGRP  E+SRTIVMES IEDIIHPDGWMPW+GD
Sbjct: 481 TTATVLQNCKILPDKTLEAVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDGD 540

Query: 541 FALKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESASVPVHA 594
           FALKTLYYAEF+N+GPGAKTDARVKWPG+KVIDK+EAAKFTIGTFL++DWI+S+SV VH 
Sbjct: 541 FALKTLYYAEFSNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSSSVSVHV 600

BLAST of Lag0042190.1 vs. NCBI nr
Match: KAG6596157.1 (putative pectinesterase/pectinesterase inhibitor 45, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 943.0 bits (2436), Expect = 1.3e-270
Identity = 474/601 (78.87%), Postives = 528/601 (87.85%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVISVSVILLVVVAAVFVQVNSNSSKSSSKD-- 60
           MVFQDFDQISERRRAERARKFR RV IAV+S  +IL++VVAAVFVQV S  +KS+++D  
Sbjct: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQV-SPPTKSTTRDGA 60

Query: 61  ------SDGNSKDPDTPETKQVSRVDKMIVMICNSTDYKGKCETTLKDGVKTDPDSSDPK 120
                 S G+SKD  T + KQVSR  KMI MICNSTDYKGKCE+TLK+GV+T PDSSDPK
Sbjct: 61  GSGSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKGKCESTLKEGVETHPDSSDPK 120

Query: 121 DLIKLAISAAATELKSAVKKAEGFNFATAEEKGAFEDCKVLFEDAVEELEISVSEMSRKN 180
           DLIKLAI+A +TE+ SA+KKA   NF T EEKGAFEDCK+L E+AVEEL+ SVSE+SRK+
Sbjct: 121 DLIKLAIAAVSTEVNSALKKASAMNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSRKD 180

Query: 181 LGKLTAKTTPDLNNWLSAVISYQETCVDGFPDGKLKSDMEKAVKSVKELTSNSLAMISQV 240
           LGKLT KTTPDLNNWLSAV+SY  TCVDGFP+GK K++ME  +K+ KELTSNSLAMISQV
Sbjct: 181 LGKLTEKTTPDLNNWLSAVMSYHATCVDGFPEGKSKTEMEATLKAGKELTSNSLAMISQV 240

Query: 241 VSFFSTFEKPDGEEAATRRRLLARDGLPIWMNTQERRMLKGATAGDKPKPNVVVAKDGSG 300
            +FFSTFEK DG  AATRRRL+A+DGLP WM + ERRMLK     DKP PNVVVAKDGSG
Sbjct: 241 ATFFSTFEKSDGGGAATRRRLMAKDGLPSWMTSYERRMLKRDAEDDKPTPNVVVAKDGSG 300

Query: 301 NFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGKKNF 360
           NFKTINEALAAMPAKY+GRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISG KN 
Sbjct: 301 NFKTINEALAAMPAKYEGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGNKNS 360

Query: 361 VDGVRTFQTATFVALGDGFLGQAIGFRNTAGPEKHQAVAARIQGDRAIFVNCRFDGYQDT 420
            DGVR FQTA+FVALGDG L + IGFRNTAGPEK QAVAAR+QGDRAIFVNCRF+G+QDT
Sbjct: 361 ADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGFQDT 420

Query: 421 LYAQAHRQYYRSCLITGTIDFIFGDAAAIFQNCNLMVRKPLDKQQNIVTAQGRTDKHETT 480
           LYAQAHRQ+YRSCLITGT+D IFGDAAAIFQNCN M++KPLDKQ N+VTAQGR+D+HETT
Sbjct: 421 LYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRHETT 480

Query: 481 GIVLQNCKILPDKTLEPVKANFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFA 540
             VLQNCKILPDKTLE VKA FKSYLGRP  E+SRTIVMES IEDIIHPDGWMPW+GDFA
Sbjct: 481 ATVLQNCKILPDKTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDGDFA 540

Query: 541 LKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESASVPVHAGL 594
           LKTLYYAEFNN+GPGAKTDARVKWPG+KVIDK+EAAKFTIGTFL++DWI+S+SV VH GL
Sbjct: 541 LKTLYYAEFNNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSSSVSVHVGL 600

BLAST of Lag0042190.1 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 1.1e-176
Identity = 321/614 (52.28%), Postives = 416/614 (67.75%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVISVSVILLVVVAAVFVQVN-SNSSKSSSKDS 60
           M FQDFD+I ER  AER RKFR+R+ + V+SV V+   ++   F  V   N ++   K +
Sbjct: 1   MAFQDFDKIQERVNAERKRKFRKRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTT 60

Query: 61  DGNSKDPDT-------------PETKQVSRVDKMIVMICNSTDYKGKCETTLKDGVKTDP 120
           +  SKD  T              +T +  +VDK+I  +CNST YK  C+ TLK+  K D 
Sbjct: 61  NNKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDT 120

Query: 121 DSSDPKDLIKLAISAAATELKSAVKKAEGFNFATAEEKGAFEDCKVLFEDAVEELEISVS 180
             +DP+ L+K AI A   +L    K+         ++K A   CK+L ++A EEL  S+ 
Sbjct: 121 PQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKTENKDDKDAIAQCKLLVDEAKEELGTSMK 180

Query: 181 EMSRKNLGKLTAKTTPDLNNWLSAVISYQETCVDGFPDGKLKSDMEKAVKSVKELTSNSL 240
            ++   +    AK  PDL++WLSAV+SYQETCVDGF +GKLK+++ K   S + LTSNSL
Sbjct: 181 RINDSEVNNF-AKIVPDLDSWLSAVMSYQETCVDGFEEGKLKTEIRKNFNSSQVLTSNSL 240

Query: 241 AMISQVVSFFSTFEKPDGEEAATRRRLLAR------DGLPIWMNTQERRMLKGATAGDKP 300
           AMI  +  + S+  K       TR  L AR      D +  W++ +ERRMLK A      
Sbjct: 241 AMIKSLDGYLSSVPK-----VKTRLLLEARSSAKETDHITSWLSNKERRMLK-AVDVKAL 300

Query: 301 KPNVVVAKDGSGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDG 360
           KPN  VAKDGSGNF TIN AL AMPAKY GRY IY+K GIY+E+V+I KK  NVTM GDG
Sbjct: 301 KPNATVAKDGSGNFTTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDG 360

Query: 361 SQKSMISGKKNFVDGVRTFQTATFVALGDGFLGQAIGFRNTAGPEKHQAVAARIQGDRAI 420
           SQK++++G K+    +RTF TATFVA G+GF+ Q++GFRNTAGPE HQAVA R+Q DR++
Sbjct: 361 SQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSV 420

Query: 421 FVNCRFDGYQDTLYAQAHRQYYRSCLITGTIDFIFGDAAAIFQNCNLMVRKPLDKQQNIV 480
           F+NCRF+GYQDTLYA  HRQYYRSC+I GT+DFIFGDAAAIFQNC++ +RK L  Q+N V
Sbjct: 421 FLNCRFEGYQDTLYAYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTV 480

Query: 481 TAQGRTDKHETTGIVLQNCKILPDKTLEPVKANFKSYLGRPWKEFSRTIVMESTIEDIIH 540
           TAQGR DK +TTG V+ NC + P++ L+PVKA FKSYLGRPWK  SRT+VMESTIED+I 
Sbjct: 481 TAQGRVDKFQTTGFVIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVID 540

Query: 541 PDGWMPW-EGDFALKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELD 594
           P GW+ W E DFA+ TL YAE+ N GP   T ARVKWPG++V++K+EA KFT+G FL+ +
Sbjct: 541 PVGWLRWQETDFAIDTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE 600

BLAST of Lag0042190.1 vs. ExPASy Swiss-Prot
Match: Q7Y201 (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3702 GN=PME13 PE=2 SV=2)

HSP 1 Score: 593.2 bits (1528), Expect = 3.3e-168
Identity = 314/622 (50.48%), Postives = 409/622 (65.76%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVISVSVILLVVVAAVFVQV------------- 60
           M FQDFD+I ER  A R RKFR+R+ +  +S+ V++  +V   F  V             
Sbjct: 1   MAFQDFDKIQERVNANRKRKFRKRIIVGTVSLLVVVAAIVGGAFAYVAYEKRNEQQQQQQ 60

Query: 61  ---NSNSSKSSS---KDSDGNSKDPDTPETK---------QVSRVDKMIVMICNSTDYKG 120
              N N S S +   KDSD  S  P TP  K         +  + DK+I  +C+ST Y  
Sbjct: 61  QAKNHNKSGSGNNVVKDSDKKSPSPPTPSQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQ 120

Query: 121 KCETTLKDGVKTDPDSSDPKDLIKLAISAAATELKSAVKKAEGFNFATAEEKGAFEDCKV 180
            CE TLK+         +P   +K AI A   +L   ++K         ++K A E CK+
Sbjct: 121 ICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKTENQDDKDAIEQCKL 180

Query: 181 LFEDAVEELEISVSEMSRKNLGKLTAKTTPDLNNWLSAVISYQETCVDGFPDGKLKSDME 240
           L EDA EE   S+++++   +     K  PDL +WLSAV+SYQETC+DGF +G LKS+++
Sbjct: 181 LVEDAKEETVASLNKINVTEVNSF-EKVVPDLESWLSAVMSYQETCLDGFEEGNLKSEVK 240

Query: 241 KAVKSVKELTSNSLAMISQVVSFFSTFEKPDGEEAATRRRLLARDGLPIWMNTQERRMLK 300
            +V S + LTSNSLA+I       S   K         R LL  D +P W++  +RRML+
Sbjct: 241 TSVNSSQVLTSNSLALIKTFTENLSPVMK------VVERHLL--DDIPSWVSNDDRRMLR 300

Query: 301 GATAGDKPKPNVVVAKDGSGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMA 360
            A      KPN  VAKDGSG+F TIN+AL AMP KY+GRY+IYVK+GIY+E V + KK A
Sbjct: 301 -AVDVKALKPNATVAKDGSGDFTTINDALRAMPEKYEGRYIIYVKQGIYDEYVTVDKKKA 360

Query: 361 NVTMYGDGSQKSMISGKKNFVDGVRTFQTATFVALGDGFLGQAIGFRNTAGPEKHQAVAA 420
           N+TM GDGSQK++++G K+    +RTF TATFVA G+GF+ Q++GFRNTAGPE HQAVA 
Sbjct: 361 NLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAI 420

Query: 421 RIQGDRAIFVNCRFDGYQDTLYAQAHRQYYRSCLITGTIDFIFGDAAAIFQNCNLMVRKP 480
           R+Q DR+IF+NCRF+GYQDTLYA  HRQYYRSC+I GTIDFIFGDAAAIFQNCN+ +RK 
Sbjct: 421 RVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGTIDFIFGDAAAIFQNCNIFIRKG 480

Query: 481 LDKQQNIVTAQGRTDKHETTGIVLQNCKILPDKTLEPVKANFKSYLGRPWKEFSRTIVME 540
           L  Q+N VTAQGR DK +TTG V+ NCKI  ++ L+PVK  +KSYLGRPWK +SRTI+ME
Sbjct: 481 LPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPVKEEYKSYLGRPWKNYSRTIIME 540

Query: 541 STIEDIIHPDGWMPW-EGDFALKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFT 594
           S IE++I P GW+ W E DFA+ TLYYAE+NN+G    T +RVKWPG+KVI+K+EA  +T
Sbjct: 541 SKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGDTTSRVKWPGFKVINKEEALNYT 600

BLAST of Lag0042190.1 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 9.3e-131
Identity = 254/567 (44.80%), Postives = 347/567 (61.20%), Query Frame = 0

Query: 32  VSVILLVVVAAVFVQVNSNSSKSSSKDSDGNSKDPDTPETKQVSRVDKMIVMICNSTDYK 91
           ++ +L+++V AV +  + N+S S            +     Q+      +  +C  TDYK
Sbjct: 18  ITALLVLMVVAVGITTSRNTSHS------------EKIVPVQIKTATTAVEAVCAPTDYK 77

Query: 92  GKCETTLKDGVKTDPDSSDPKDLIKLAISAAATELKSAVKKAEGFNFATA----EEKGAF 151
             C  +L   +K  PDS+ P DLIKL  +     ++ ++KKA     A A    + KGA 
Sbjct: 78  ETCVNSL---MKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGAL 137

Query: 152 EDCKVLFEDAVEELEISVSEMSRKNLGKLTAKTTPDLNNWLSAVISYQETCVDGFPD--G 211
           E C+ L  DA ++L+  +      ++ ++      DL  WLS  I+YQ+TC+D F +   
Sbjct: 138 ELCEKLMNDATDDLKKCLDNFDGFSIPQI-EDFVEDLRVWLSGSIAYQQTCMDTFEETNS 197

Query: 212 KLKSDMEKAVKSVKELTSNSLAMISQVVSFFSTFEKP--DGEEAATRRRLL-ARDGLPIW 271
           KL  DM+K  K+ +ELTSN LAMI+ + +    F      G+     R+LL A DG+P W
Sbjct: 198 KLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDGIPSW 257

Query: 272 MNTQERRMLKGATAGDKPKPNVVVAKDGSGNFKTINEALAAMPAKYDGRYVIYVKEGIYE 331
           +    RR++  AT G   K NVVVA DGSG +KTINEAL A+P      +VIY+K+G+Y 
Sbjct: 258 VGPNTRRLM--ATKGG-VKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYN 317

Query: 332 ETVVITKKMANVTMYGDGSQKSMISGKKNFVDG-VRTFQTATFVALGDGFLGQAIGFRNT 391
           E V +TKKM +VT  GDG  K+ I+G  N+  G V+T+ TAT    GD F  + IGF NT
Sbjct: 318 EKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENT 377

Query: 392 AGPEKHQAVAARIQGDRAIFVNCRFDGYQDTLYAQAHRQYYRSCLITGTIDFIFGDAAAI 451
           AGPE HQAVA R+  D A+F NC+ DGYQDTLY  +HRQ++R C ++GT+DFIFGD   +
Sbjct: 378 AGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVV 437

Query: 452 FQNCNLMVRKPLDKQQNIVTAQGRTDKHETTGIVLQNCKILPDKTLEPVKANFKSYLGRP 511
            QNCN++VRKP+  Q  ++TAQGR+DK E+TG+VLQNC I  +    PVK+  K+YLGRP
Sbjct: 438 LQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRP 497

Query: 512 WKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYYAEFNNQGPGAKTDARVKWPGYKV 571
           WKEFSRTI+M +TI+D+I P GW+PW GDFAL TLYYAE+ N GPG+    RVKWPG K 
Sbjct: 498 WKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKK 557

Query: 572 IDKDEAAKFTIGTFLELD-WIESASVP 588
           +   +A +FT   FL  + WI    VP
Sbjct: 558 LSPKQALRFTPARFLRGNLWIPPNRVP 565

BLAST of Lag0042190.1 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 461.8 bits (1187), Expect = 1.1e-128
Identity = 246/582 (42.27%), Postives = 356/582 (61.17%), Query Frame = 0

Query: 16  ERARKFRQRVTIAVISVSVILLVVVAAVFVQVNSNSSKSSSKDSDGNSKDPDTPETKQVS 75
           + +++ R+ + I + SV +I +VV   V V +N +         DG+SK        +V+
Sbjct: 7   DESKRKRRYIVITISSVLLISMVVAVTVGVSLNKH---------DGDSKG-----KAEVN 66

Query: 76  RVDKMIVMICNSTDYKGKCETTLKDGVKTDPDSSDPKDLIKLAISAAATELKSAVKKAEG 135
              K +  +C  TDY+  CE TL   +K   +++DP +L+K A +    ++  A KK++ 
Sbjct: 67  ASVKAVKDVCAPTDYRKTCEDTL---IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQT 126

Query: 136 FNFATAEEKG--AFEDCKVLFEDAVEELEISVSEMSRKNLGKLTAKTTPDLNNWLSAVIS 195
                 + +   A + CK L + A++EL  S  E+ +     L  +   +L  WLSA IS
Sbjct: 127 IMELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEF-HLLDEALINLRIWLSAAIS 186

Query: 196 YQETCVDGF--PDGKLKSDMEKAVKSVKELTSNSLAMISQVVSFFSTFEKPDGEEAATRR 255
           ++ETC++GF    G     M+KA+K+  ELT N LA+IS++ +F    + P        R
Sbjct: 187 HEETCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIP----GLNSR 246

Query: 256 RLLARDGLPIWMNTQERRMLKGATAGDKPKPNVVVAKDGSGNFKTINEALAAMPAKYDGR 315
           RLLA +G P W++ + R++L+ A A    KP++VVA+DGSG +KTINEAL  +P K +  
Sbjct: 247 RLLA-EGFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTT 306

Query: 316 YVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGKKNFVDGVRTFQTATFVALGDGF 375
           +V+++K G+Y+E V + K M+++   GDG  K++ISG KN+ DG+ T++TAT   +G+ F
Sbjct: 307 FVVHIKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYF 366

Query: 376 LGQAIGFRNTAGPEKHQAVAARIQGDRAIFVNCRFDGYQDTLYAQAHRQYYRSCLITGTI 435
           + + IGF NTAG  KHQAVA R+Q D +IF NCRFDGYQDTLY  +HRQ++R C I+GTI
Sbjct: 367 IAKNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTI 426

Query: 436 DFIFGDAAAIFQNCNLMVRKPLDKQQNIVTAQGRTDKHETTGIVLQNCKILPDKTLEPVK 495
           DF+FGDAAA+FQNC L+VRKPL  Q   +TA GR D  E+TG V Q C I  +     VK
Sbjct: 427 DFLFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVK 486

Query: 496 ANFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYYAEFNNQGPGAKTD 555
              K+YLGRPWKE+SRTI+M + I D + P GW PW GDF LKTL+Y+E  N GPG+   
Sbjct: 487 ETSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALA 546

Query: 556 ARVKWPGYKVIDKDEAAKFTIGTFLE-LDWIESASVPVHAGL 593
            RV W G K + +++  KFT   +++  DWI    VP   GL
Sbjct: 547 NRVTWAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of Lag0042190.1 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 7.4e-128
Identity = 246/583 (42.20%), Postives = 355/583 (60.89%), Query Frame = 0

Query: 16  ERARKFRQRVTIAVISVSVILLVVVAAVFVQVNSNSSKSSSKDSDGNSKDPDTPETKQVS 75
           E A++ ++ V I++ SV +I +VV   + V VN +                D    ++++
Sbjct: 8   EDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKS----------------DNAGDEEIT 67

Query: 76  RVDKMIVMICNSTDYKGKCETTLKDGVKTDPDSSDPKDLIKLAISAAATELKSAVKKAEG 135
              K I  +C  TDYK  CE TL+   K   D+SDP +L+K A +A   ++    KK++ 
Sbjct: 68  TSVKAIKDVCAPTDYKETCEDTLRKDAK---DTSDPLELVKTAFNATMKQISDVAKKSQT 127

Query: 136 FNFATAE--EKGAFEDCKVLFEDAVEELEISVSEMSRKNLGKLTAKTTPDLNNWLSAVIS 195
                 +   K A + CK L + A+ EL  S  E+ +    K+  +    L  WLSA IS
Sbjct: 128 MIELQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVD-EALVKLRIWLSATIS 187

Query: 196 YQETCVDGF--PDGKLKSDMEKAVKSVKELTSNSLAMISQVVSFFSTFEKPDGEEAATRR 255
           +++TC+DGF    G     ++KA+K+  +LT N LAM++++ ++    + P+       R
Sbjct: 188 HEQTCLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPE----MNSR 247

Query: 256 RLLARDGLPIWMNTQERRMLKGATAGDKPKPNVVVAKDGSGNFKTINEALAAMPAKYDGR 315
           RLL+++  P WM+ + RR+L    +  + KP++VVA+DGSG +KTINEAL  +P K +  
Sbjct: 248 RLLSQE-FPSWMDARARRLLNAPMS--EVKPDIVVAQDGSGQYKTINEALNFVPKKKNTT 307

Query: 316 YVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGKKNFVDGVRTFQTATFVALGDGF 375
           +V+++KEGIY+E V + + M ++   GDG  K++ISG K++ DG+ T++TAT   +GD F
Sbjct: 308 FVVHIKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHF 367

Query: 376 LGQAIGFRNTAGPEKHQAVAARIQGDRAIFVNCRFDGYQDTLYAQAHRQYYRSCLITGTI 435
           + + I F NTAG  KHQAVA R+  D +IF NC+FDGYQDTLYA +HRQ+YR C I+GTI
Sbjct: 368 IAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTI 427

Query: 436 DFIFGDAAAIFQNCNLMVRKPLDKQQNIVTAQGRTDKHETTGIVLQNCKILPDKTLEPVK 495
           DF+FGDAAA+FQNC L+VRKPL  Q   +TA GR D  E+TG VLQ C I+ +     VK
Sbjct: 428 DFLFGDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVK 487

Query: 496 ANFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYYAEFNNQGPGAKTD 555
              K+YLGRPWKE+SRTI+M + I D + P+GW PW G+F L TL+Y+E  N GPGA   
Sbjct: 488 EQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAIT 547

Query: 556 ARVKWPGYKVIDKDEAAKFTIGTFLELD-WIESASVPVHAGLF 594
            RV WPG K +  +E  KFT   +++ D WI    VP   GLF
Sbjct: 548 KRVTWPGIKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGLF 563

BLAST of Lag0042190.1 vs. ExPASy TrEMBL
Match: A0A5D3BLU5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2460G00020 PE=3 SV=1)

HSP 1 Score: 1030.8 bits (2664), Expect = 2.3e-297
Identity = 518/598 (86.62%), Postives = 555/598 (92.81%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVISVSVILLVVVAAVFVQVNSNSSKSSSKDSD 60
           MVFQDFDQISERRRAERARKFR+RVTIAV+S SVILLVV AAVFVQV S+SSKS++K+ D
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLVVAAAVFVQV-SSSSKSTTKNGD 60

Query: 61  -----GNSKDPDTPETKQVSRVDKMIVMICNSTDYKGKCETTLKDGVKTDPDSSDPKDLI 120
                GNSK P  PETKQVSRV+KMI MICNSTDYKGKCE+TLKDG+ TDP+SSDPKDLI
Sbjct: 61  SGSSTGNSKKPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGLHTDPNSSDPKDLI 120

Query: 121 KLAISAAATELKSAVKKAEGFNFATAEEKGAFEDCKVLFEDAVEELEISVSEMSRKNLGK 180
           KLAISAAA E+KSAVKKA GFNFAT EEKGAFEDCKVL EDAVEELE+S+SE+S+KN+GK
Sbjct: 121 KLAISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGK 180

Query: 181 LTAKTTPDLNNWLSAVISYQETCVDGFPDGKLKSDMEKAVKSVKELTSNSLAMISQVVSF 240
           LTAKTTPDLNNWLSAV+SY ETCVDGFP+GK+KS+MEK VK+ KELTSNSLAMISQV SF
Sbjct: 181 LTAKTTPDLNNWLSAVMSYHETCVDGFPEGKMKSEMEKVVKAGKELTSNSLAMISQVASF 240

Query: 241 FSTFEKPDGEEAATRRRLLARDGLPIWMNTQERRMLKGATAGDKPKPNVVVAKDGSGNFK 300
           FSTFE P+G  AA+RRRL+A  G+P WMN+ ERRMLKGA AG+KPKPNVVVAKDGSG FK
Sbjct: 241 FSTFEMPEG--AASRRRLMATKGVPTWMNSNERRMLKGAAAGEKPKPNVVVAKDGSGEFK 300

Query: 301 TINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGKKNFVDG 360
           TINEALAAMPAKYDGRYVIYVKEGIY+ETVVITKKM NVTMYGDGSQKSMISG KNFVDG
Sbjct: 301 TINEALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFVDG 360

Query: 361 VRTFQTATFVALGDGFLGQAIGFRNTAGPEKHQAVAARIQGDRAIFVNCRFDGYQDTLYA 420
           VRTFQTATFVALG+GFLGQAIGFRN AGPEKHQAVAAR+Q DRAIFVNCRF+GYQDTLYA
Sbjct: 361 VRTFQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYA 420

Query: 421 QAHRQYYRSCLITGTIDFIFGDAAAIFQNCNLMVRKPLDKQQNIVTAQGRTDKHETTGIV 480
           QAHRQ+YRSCLITGTIDFIFGDAAAIFQNCN+M+RKPLD QQNIVTAQGRTDKHETTGIV
Sbjct: 421 QAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNIVTAQGRTDKHETTGIV 480

Query: 481 LQNCKILPDKTLEPVKANFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKT 540
           LQNCKILPDKTLE VK+ FKSYLGRPWKEFSRTIVMES IED+IHPDGWM W+GDFALKT
Sbjct: 481 LQNCKILPDKTLEAVKSQFKSYLGRPWKEFSRTIVMESKIEDVIHPDGWMAWQGDFALKT 540

Query: 541 LYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESASVPVHAGLF 594
           LYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIES S PVH GLF
Sbjct: 541 LYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHVGLF 595

BLAST of Lag0042190.1 vs. ExPASy TrEMBL
Match: A0A1S3CCA7 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499351 PE=3 SV=1)

HSP 1 Score: 1030.8 bits (2664), Expect = 2.3e-297
Identity = 518/598 (86.62%), Postives = 555/598 (92.81%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVISVSVILLVVVAAVFVQVNSNSSKSSSKDSD 60
           MVFQDFDQISERRRAERARKFR+RVTIAV+S SVILLVV AAVFVQV S+SSKS++K+ D
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLVVAAAVFVQV-SSSSKSTTKNGD 60

Query: 61  -----GNSKDPDTPETKQVSRVDKMIVMICNSTDYKGKCETTLKDGVKTDPDSSDPKDLI 120
                GNSK P  PETKQVSRV+KMI MICNSTDYKGKCE+TLKDG+ TDP+SSDPKDLI
Sbjct: 61  SGSSTGNSKKPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGLHTDPNSSDPKDLI 120

Query: 121 KLAISAAATELKSAVKKAEGFNFATAEEKGAFEDCKVLFEDAVEELEISVSEMSRKNLGK 180
           KLAISAAA E+KSAVKKA GFNFAT EEKGAFEDCKVL EDAVEELE+S+SE+S+KN+GK
Sbjct: 121 KLAISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSEVSKKNMGK 180

Query: 181 LTAKTTPDLNNWLSAVISYQETCVDGFPDGKLKSDMEKAVKSVKELTSNSLAMISQVVSF 240
           LTAKTTPDLNNWLSAV+SY ETCVDGFP+GK+KS+MEK VK+ KELTSNSLAMISQV SF
Sbjct: 181 LTAKTTPDLNNWLSAVMSYHETCVDGFPEGKMKSEMEKVVKAGKELTSNSLAMISQVASF 240

Query: 241 FSTFEKPDGEEAATRRRLLARDGLPIWMNTQERRMLKGATAGDKPKPNVVVAKDGSGNFK 300
           FSTFE P+G  AA+RRRL+A  G+P WMN+ ERRMLKGA AG+KPKPNVVVAKDGSG FK
Sbjct: 241 FSTFEMPEG--AASRRRLMATKGVPTWMNSNERRMLKGAAAGEKPKPNVVVAKDGSGEFK 300

Query: 301 TINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGKKNFVDG 360
           TINEALAAMPAKYDGRYVIYVKEGIY+ETVVITKKM NVTMYGDGSQKSMISG KNFVDG
Sbjct: 301 TINEALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFVDG 360

Query: 361 VRTFQTATFVALGDGFLGQAIGFRNTAGPEKHQAVAARIQGDRAIFVNCRFDGYQDTLYA 420
           VRTFQTATFVALG+GFLGQAIGFRN AGPEKHQAVAAR+Q DRAIFVNCRF+GYQDTLYA
Sbjct: 361 VRTFQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYA 420

Query: 421 QAHRQYYRSCLITGTIDFIFGDAAAIFQNCNLMVRKPLDKQQNIVTAQGRTDKHETTGIV 480
           QAHRQ+YRSCLITGTIDFIFGDAAAIFQNCN+M+RKPLD QQNIVTAQGRTDKHETTGIV
Sbjct: 421 QAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNIVTAQGRTDKHETTGIV 480

Query: 481 LQNCKILPDKTLEPVKANFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKT 540
           LQNCKILPDKTLE VK+ FKSYLGRPWKEFSRTIVMES IED+IHPDGWM W+GDFALKT
Sbjct: 481 LQNCKILPDKTLEAVKSQFKSYLGRPWKEFSRTIVMESKIEDVIHPDGWMAWQGDFALKT 540

Query: 541 LYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESASVPVHAGLF 594
           LYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIES S PVH GLF
Sbjct: 541 LYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHVGLF 595

BLAST of Lag0042190.1 vs. ExPASy TrEMBL
Match: A0A0A0KX33 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G630740 PE=3 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 3.6e-295
Identity = 509/596 (85.40%), Postives = 551/596 (92.45%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVISVSVILLVVVAAVFVQVNSNSSKSSSKD-- 60
           MVFQDFDQISERRRAERARKFR+RV IAV+S SVILLVV AAVFVQV+S+ S + + D  
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVIIAVVSASVILLVVAAAVFVQVSSSKSTTKNDDSG 60

Query: 61  -SDGNSKDPDTPETKQVSRVDKMIVMICNSTDYKGKCETTLKDGVKTDPDSSDPKDLIKL 120
            S GNSK P  PETKQVSRV+KMI MICNSTDYKGKCE+TLKDGV TDP+SSDPKDLIKL
Sbjct: 61  SSSGNSKQPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGVHTDPNSSDPKDLIKL 120

Query: 121 AISAAATELKSAVKKAEGFNFATAEEKGAFEDCKVLFEDAVEELEISVSEMSRKNLGKLT 180
           AISAAA E+KSAVKKA GFNFAT EEKGAFEDCKVL EDA+EELE+S+SE+++KN+GKLT
Sbjct: 121 AISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAIEELEMSMSEVNKKNMGKLT 180

Query: 181 AKTTPDLNNWLSAVISYQETCVDGFPDGKLKSDMEKAVKSVKELTSNSLAMISQVVSFFS 240
           AKTTP+LNNWLSAV+SY ETCVDGFP+GK+KSD+EK VK+ KELTSNSLAMISQV SFFS
Sbjct: 181 AKTTPNLNNWLSAVMSYHETCVDGFPEGKMKSDIEKVVKAGKELTSNSLAMISQVASFFS 240

Query: 241 TFEKPDGEEAATRRRLLARDGLPIWMNTQERRMLKGATAGDKPKPNVVVAKDGSGNFKTI 300
           TFE P+G  AA+RRRL+  +G+P WM+  +RRMLKGA AG+KPKPNVVVAKDGSG FKTI
Sbjct: 241 TFEMPEG--AASRRRLMTTNGVPTWMDRNQRRMLKGAAAGEKPKPNVVVAKDGSGEFKTI 300

Query: 301 NEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGKKNFVDGVR 360
           NEALAAMPAKYDGRYVIYVKEGIY+ETVVITKKM NVTMYGDGSQKSMISG KNFVDGVR
Sbjct: 301 NEALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSMISGSKNFVDGVR 360

Query: 361 TFQTATFVALGDGFLGQAIGFRNTAGPEKHQAVAARIQGDRAIFVNCRFDGYQDTLYAQA 420
           TFQTATFVALG+GFLGQAIGFRN AGPEKHQAVAAR+Q DRAIFVNCRF+GYQDTLY QA
Sbjct: 361 TFQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCRFEGYQDTLYTQA 420

Query: 421 HRQYYRSCLITGTIDFIFGDAAAIFQNCNLMVRKPLDKQQNIVTAQGRTDKHETTGIVLQ 480
           HRQ+YRSCLITGTIDFIFGDAAAIFQNCN+M+RKPLD QQN+VTAQGRTDKHETTGIVLQ
Sbjct: 421 HRQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNMVTAQGRTDKHETTGIVLQ 480

Query: 481 NCKILPDKTLEPVKANFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLY 540
           NCKILPDKTLEPVK+ FKSYLGRPWKEFSRTIVMESTIED+IHPDGWM WEGDFALKTLY
Sbjct: 481 NCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDVIHPDGWMAWEGDFALKTLY 540

Query: 541 YAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESASVPVHAGLF 594
           YAEFNN+GPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIES S PVH GLF
Sbjct: 541 YAEFNNKGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTSAPVHVGLF 594

BLAST of Lag0042190.1 vs. ExPASy TrEMBL
Match: A0A6J1I3R6 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470280 PE=3 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 2.5e-272
Identity = 476/603 (78.94%), Postives = 526/603 (87.23%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVISVSVILLVVVAAVFVQVN----------SN 60
           MVFQDFDQISERRRAERARKFR RV IAV+S  +IL+ VVAAVFVQV+          + 
Sbjct: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVFVVAAVFVQVSPPTKSTTRDGAG 60

Query: 61  SSKSSSKDSDGNSKDPDTPETKQVSRVDKMIVMICNSTDYKGKCETTLKDGVKTDPDSSD 120
           +  SSS  S G+SKD  T + KQVSR  KMI MICNSTDYKGKCE+TLK GV+T PDSSD
Sbjct: 61  AGSSSSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKGKCESTLKQGVETHPDSSD 120

Query: 121 PKDLIKLAISAAATELKSAVKKAEGFNFATAEEKGAFEDCKVLFEDAVEELEISVSEMSR 180
           PKDLIKLAI+A +TE+ SA+KKA  FNF T EEKGAFEDCK+L E+AVEEL+ SVSE+SR
Sbjct: 121 PKDLIKLAIAAVSTEVNSALKKASAFNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSR 180

Query: 181 KNLGKLTAKTTPDLNNWLSAVISYQETCVDGFPDGKLKSDMEKAVKSVKELTSNSLAMIS 240
           K+LGKLT KTTPDLNNWLSAV+SY  TCVDGFP+GK K+ ME  +K+ KELTSNSLAMIS
Sbjct: 181 KDLGKLTDKTTPDLNNWLSAVMSYHATCVDGFPEGKSKTVMEATLKAGKELTSNSLAMIS 240

Query: 241 QVVSFFSTFEKPDGEEAATRRRLLARDGLPIWMNTQERRMLKGATAGDKPKPNVVVAKDG 300
           QV +FFSTFEK DG  AATRRRL+A+DGLP WM + ERRMLKG  A DKP PNVVVAKDG
Sbjct: 241 QVATFFSTFEKSDGGGAATRRRLMAKDGLPSWMTSNERRMLKGDAADDKPTPNVVVAKDG 300

Query: 301 SGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGKK 360
           SGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISG K
Sbjct: 301 SGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGNK 360

Query: 361 NFVDGVRTFQTATFVALGDGFLGQAIGFRNTAGPEKHQAVAARIQGDRAIFVNCRFDGYQ 420
           N  DGVR FQTA+FVALGDG L + IGFRNTAGPEK QAVAAR+QGDRAIFVNCRF+G+Q
Sbjct: 361 NSADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGFQ 420

Query: 421 DTLYAQAHRQYYRSCLITGTIDFIFGDAAAIFQNCNLMVRKPLDKQQNIVTAQGRTDKHE 480
           DTLYAQAHRQ+YRSCLITGT+D IFGDAAAIFQNCN M++KPLDKQ N+VTAQGR+D+HE
Sbjct: 421 DTLYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRHE 480

Query: 481 TTGIVLQNCKILPDKTLEPVKANFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGD 540
           TT  VLQNCKILPDKTLE VKA FKSYLGRP  E+SRTIVMES IEDIIHPDGWMPW+GD
Sbjct: 481 TTATVLQNCKILPDKTLEAVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDGD 540

Query: 541 FALKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESASVPVHA 594
           FALKTLYYAEF+N+GPGAKTDARVKWPG+KVIDK+EAAKFTIGTFL++DWI+S+SV VH 
Sbjct: 541 FALKTLYYAEFSNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSSSVSVHV 600

BLAST of Lag0042190.1 vs. ExPASy TrEMBL
Match: A0A6J1HGL4 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111463347 PE=3 SV=1)

HSP 1 Score: 941.0 bits (2431), Expect = 2.4e-270
Identity = 474/601 (78.87%), Postives = 526/601 (87.52%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVISVSVILLVVVAAVFVQVNSNSSKSSSKD-- 60
           MVFQDFDQISERRRAERARKFR RV IAV+S  +IL++VVAAVFVQV S  +KS+++D  
Sbjct: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQV-SPPTKSTTRDGA 60

Query: 61  ------SDGNSKDPDTPETKQVSRVDKMIVMICNSTDYKGKCETTLKDGVKTDPDSSDPK 120
                 S G+SKD  T + KQVSR  KMI MICNSTDYK KCE+TLK+GV+T PDSSDPK
Sbjct: 61  GSGSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKAKCESTLKEGVETHPDSSDPK 120

Query: 121 DLIKLAISAAATELKSAVKKAEGFNFATAEEKGAFEDCKVLFEDAVEELEISVSEMSRKN 180
           DLIKLAI+A +TE+ SA+KKA   NF T EEKGAFEDCK+L E+AVEEL+ SVSE+SRK+
Sbjct: 121 DLIKLAIAAVSTEVNSALKKASAMNFPTPEEKGAFEDCKLLLEEAVEELDRSVSELSRKD 180

Query: 181 LGKLTAKTTPDLNNWLSAVISYQETCVDGFPDGKLKSDMEKAVKSVKELTSNSLAMISQV 240
           LGKLT KTTPDLNNWLSAV+SY  TCVDGFP GK K++ME  +K+ KELTSNSLAMISQV
Sbjct: 181 LGKLTEKTTPDLNNWLSAVMSYHATCVDGFPGGKSKTEMEATLKAGKELTSNSLAMISQV 240

Query: 241 VSFFSTFEKPDGEEAATRRRLLARDGLPIWMNTQERRMLKGATAGDKPKPNVVVAKDGSG 300
            +FFSTFEK DG  AATRRRL+A+DGLP WM + ERRMLK     DKP PNVVVAKDGSG
Sbjct: 241 ATFFSTFEKSDGGGAATRRRLMAKDGLPSWMTSYERRMLKRDAEDDKPTPNVVVAKDGSG 300

Query: 301 NFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGKKNF 360
           NFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISG KN 
Sbjct: 301 NFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGNKNS 360

Query: 361 VDGVRTFQTATFVALGDGFLGQAIGFRNTAGPEKHQAVAARIQGDRAIFVNCRFDGYQDT 420
            DGVR FQTA+FVALGDG L + IGFRNTAGPEK QAVAAR+QGDRAIFVNCRF+G+QDT
Sbjct: 361 ADGVRAFQTASFVALGDGLLAKGIGFRNTAGPEKQQAVAARVQGDRAIFVNCRFEGFQDT 420

Query: 421 LYAQAHRQYYRSCLITGTIDFIFGDAAAIFQNCNLMVRKPLDKQQNIVTAQGRTDKHETT 480
           LYAQAHRQ+YRSCLITGT+D IFGDAAAIFQNCN M++KPLDKQ N+VTAQGR+D+HETT
Sbjct: 421 LYAQAHRQFYRSCLITGTVDIIFGDAAAIFQNCNFMLKKPLDKQANVVTAQGRSDRHETT 480

Query: 481 GIVLQNCKILPDKTLEPVKANFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFA 540
             VLQNCKILPDKTLE VKA FKSYLGRP  E+SRTIVMES IEDIIHPDGWMPW+GDFA
Sbjct: 481 ATVLQNCKILPDKTLEGVKAQFKSYLGRPRNEYSRTIVMESEIEDIIHPDGWMPWDGDFA 540

Query: 541 LKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESASVPVHAGL 594
           LKTLYYAEFNN+GPGAKTDARVKWPG+KVIDK+EAAKFTIGTFL++DWI+S+SV VH GL
Sbjct: 541 LKTLYYAEFNNKGPGAKTDARVKWPGFKVIDKEEAAKFTIGTFLDVDWIQSSSVSVHVGL 600

BLAST of Lag0042190.1 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 621.3 bits (1601), Expect = 7.9e-178
Identity = 321/614 (52.28%), Postives = 416/614 (67.75%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVISVSVILLVVVAAVFVQVN-SNSSKSSSKDS 60
           M FQDFD+I ER  AER RKFR+R+ + V+SV V+   ++   F  V   N ++   K +
Sbjct: 1   MAFQDFDKIQERVNAERKRKFRKRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTT 60

Query: 61  DGNSKDPDT-------------PETKQVSRVDKMIVMICNSTDYKGKCETTLKDGVKTDP 120
           +  SKD  T              +T +  +VDK+I  +CNST YK  C+ TLK+  K D 
Sbjct: 61  NNKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDT 120

Query: 121 DSSDPKDLIKLAISAAATELKSAVKKAEGFNFATAEEKGAFEDCKVLFEDAVEELEISVS 180
             +DP+ L+K AI A   +L    K+         ++K A   CK+L ++A EEL  S+ 
Sbjct: 121 PQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKTENKDDKDAIAQCKLLVDEAKEELGTSMK 180

Query: 181 EMSRKNLGKLTAKTTPDLNNWLSAVISYQETCVDGFPDGKLKSDMEKAVKSVKELTSNSL 240
            ++   +    AK  PDL++WLSAV+SYQETCVDGF +GKLK+++ K   S + LTSNSL
Sbjct: 181 RINDSEVNNF-AKIVPDLDSWLSAVMSYQETCVDGFEEGKLKTEIRKNFNSSQVLTSNSL 240

Query: 241 AMISQVVSFFSTFEKPDGEEAATRRRLLAR------DGLPIWMNTQERRMLKGATAGDKP 300
           AMI  +  + S+  K       TR  L AR      D +  W++ +ERRMLK A      
Sbjct: 241 AMIKSLDGYLSSVPK-----VKTRLLLEARSSAKETDHITSWLSNKERRMLK-AVDVKAL 300

Query: 301 KPNVVVAKDGSGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMANVTMYGDG 360
           KPN  VAKDGSGNF TIN AL AMPAKY GRY IY+K GIY+E+V+I KK  NVTM GDG
Sbjct: 301 KPNATVAKDGSGNFTTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDG 360

Query: 361 SQKSMISGKKNFVDGVRTFQTATFVALGDGFLGQAIGFRNTAGPEKHQAVAARIQGDRAI 420
           SQK++++G K+    +RTF TATFVA G+GF+ Q++GFRNTAGPE HQAVA R+Q DR++
Sbjct: 361 SQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSV 420

Query: 421 FVNCRFDGYQDTLYAQAHRQYYRSCLITGTIDFIFGDAAAIFQNCNLMVRKPLDKQQNIV 480
           F+NCRF+GYQDTLYA  HRQYYRSC+I GT+DFIFGDAAAIFQNC++ +RK L  Q+N V
Sbjct: 421 FLNCRFEGYQDTLYAYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTV 480

Query: 481 TAQGRTDKHETTGIVLQNCKILPDKTLEPVKANFKSYLGRPWKEFSRTIVMESTIEDIIH 540
           TAQGR DK +TTG V+ NC + P++ L+PVKA FKSYLGRPWK  SRT+VMESTIED+I 
Sbjct: 481 TAQGRVDKFQTTGFVIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVID 540

Query: 541 PDGWMPW-EGDFALKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELD 594
           P GW+ W E DFA+ TL YAE+ N GP   T ARVKWPG++V++K+EA KFT+G FL+ +
Sbjct: 541 PVGWLRWQETDFAIDTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE 600

BLAST of Lag0042190.1 vs. TAIR 10
Match: AT2G26450.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 593.2 bits (1528), Expect = 2.3e-169
Identity = 314/622 (50.48%), Postives = 409/622 (65.76%), Query Frame = 0

Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVISVSVILLVVVAAVFVQV------------- 60
           M FQDFD+I ER  A R RKFR+R+ +  +S+ V++  +V   F  V             
Sbjct: 1   MAFQDFDKIQERVNANRKRKFRKRIIVGTVSLLVVVAAIVGGAFAYVAYEKRNEQQQQQQ 60

Query: 61  ---NSNSSKSSS---KDSDGNSKDPDTPETK---------QVSRVDKMIVMICNSTDYKG 120
              N N S S +   KDSD  S  P TP  K         +  + DK+I  +C+ST Y  
Sbjct: 61  QAKNHNKSGSGNNVVKDSDKKSPSPPTPSQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQ 120

Query: 121 KCETTLKDGVKTDPDSSDPKDLIKLAISAAATELKSAVKKAEGFNFATAEEKGAFEDCKV 180
            CE TLK+         +P   +K AI A   +L   ++K         ++K A E CK+
Sbjct: 121 ICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKTENQDDKDAIEQCKL 180

Query: 181 LFEDAVEELEISVSEMSRKNLGKLTAKTTPDLNNWLSAVISYQETCVDGFPDGKLKSDME 240
           L EDA EE   S+++++   +     K  PDL +WLSAV+SYQETC+DGF +G LKS+++
Sbjct: 181 LVEDAKEETVASLNKINVTEVNSF-EKVVPDLESWLSAVMSYQETCLDGFEEGNLKSEVK 240

Query: 241 KAVKSVKELTSNSLAMISQVVSFFSTFEKPDGEEAATRRRLLARDGLPIWMNTQERRMLK 300
            +V S + LTSNSLA+I       S   K         R LL  D +P W++  +RRML+
Sbjct: 241 TSVNSSQVLTSNSLALIKTFTENLSPVMK------VVERHLL--DDIPSWVSNDDRRMLR 300

Query: 301 GATAGDKPKPNVVVAKDGSGNFKTINEALAAMPAKYDGRYVIYVKEGIYEETVVITKKMA 360
            A      KPN  VAKDGSG+F TIN+AL AMP KY+GRY+IYVK+GIY+E V + KK A
Sbjct: 301 -AVDVKALKPNATVAKDGSGDFTTINDALRAMPEKYEGRYIIYVKQGIYDEYVTVDKKKA 360

Query: 361 NVTMYGDGSQKSMISGKKNFVDGVRTFQTATFVALGDGFLGQAIGFRNTAGPEKHQAVAA 420
           N+TM GDGSQK++++G K+    +RTF TATFVA G+GF+ Q++GFRNTAGPE HQAVA 
Sbjct: 361 NLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAI 420

Query: 421 RIQGDRAIFVNCRFDGYQDTLYAQAHRQYYRSCLITGTIDFIFGDAAAIFQNCNLMVRKP 480
           R+Q DR+IF+NCRF+GYQDTLYA  HRQYYRSC+I GTIDFIFGDAAAIFQNCN+ +RK 
Sbjct: 421 RVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGTIDFIFGDAAAIFQNCNIFIRKG 480

Query: 481 LDKQQNIVTAQGRTDKHETTGIVLQNCKILPDKTLEPVKANFKSYLGRPWKEFSRTIVME 540
           L  Q+N VTAQGR DK +TTG V+ NCKI  ++ L+PVK  +KSYLGRPWK +SRTI+ME
Sbjct: 481 LPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPVKEEYKSYLGRPWKNYSRTIIME 540

Query: 541 STIEDIIHPDGWMPW-EGDFALKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFT 594
           S IE++I P GW+ W E DFA+ TLYYAE+NN+G    T +RVKWPG+KVI+K+EA  +T
Sbjct: 541 SKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGDTTSRVKWPGFKVINKEEALNYT 600

BLAST of Lag0042190.1 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 468.8 bits (1205), Expect = 6.6e-132
Identity = 254/567 (44.80%), Postives = 347/567 (61.20%), Query Frame = 0

Query: 32  VSVILLVVVAAVFVQVNSNSSKSSSKDSDGNSKDPDTPETKQVSRVDKMIVMICNSTDYK 91
           ++ +L+++V AV +  + N+S S            +     Q+      +  +C  TDYK
Sbjct: 18  ITALLVLMVVAVGITTSRNTSHS------------EKIVPVQIKTATTAVEAVCAPTDYK 77

Query: 92  GKCETTLKDGVKTDPDSSDPKDLIKLAISAAATELKSAVKKAEGFNFATA----EEKGAF 151
             C  +L   +K  PDS+ P DLIKL  +     ++ ++KKA     A A    + KGA 
Sbjct: 78  ETCVNSL---MKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGAL 137

Query: 152 EDCKVLFEDAVEELEISVSEMSRKNLGKLTAKTTPDLNNWLSAVISYQETCVDGFPD--G 211
           E C+ L  DA ++L+  +      ++ ++      DL  WLS  I+YQ+TC+D F +   
Sbjct: 138 ELCEKLMNDATDDLKKCLDNFDGFSIPQI-EDFVEDLRVWLSGSIAYQQTCMDTFEETNS 197

Query: 212 KLKSDMEKAVKSVKELTSNSLAMISQVVSFFSTFEKP--DGEEAATRRRLL-ARDGLPIW 271
           KL  DM+K  K+ +ELTSN LAMI+ + +    F      G+     R+LL A DG+P W
Sbjct: 198 KLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDGIPSW 257

Query: 272 MNTQERRMLKGATAGDKPKPNVVVAKDGSGNFKTINEALAAMPAKYDGRYVIYVKEGIYE 331
           +    RR++  AT G   K NVVVA DGSG +KTINEAL A+P      +VIY+K+G+Y 
Sbjct: 258 VGPNTRRLM--ATKGG-VKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYN 317

Query: 332 ETVVITKKMANVTMYGDGSQKSMISGKKNFVDG-VRTFQTATFVALGDGFLGQAIGFRNT 391
           E V +TKKM +VT  GDG  K+ I+G  N+  G V+T+ TAT    GD F  + IGF NT
Sbjct: 318 EKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENT 377

Query: 392 AGPEKHQAVAARIQGDRAIFVNCRFDGYQDTLYAQAHRQYYRSCLITGTIDFIFGDAAAI 451
           AGPE HQAVA R+  D A+F NC+ DGYQDTLY  +HRQ++R C ++GT+DFIFGD   +
Sbjct: 378 AGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVV 437

Query: 452 FQNCNLMVRKPLDKQQNIVTAQGRTDKHETTGIVLQNCKILPDKTLEPVKANFKSYLGRP 511
            QNCN++VRKP+  Q  ++TAQGR+DK E+TG+VLQNC I  +    PVK+  K+YLGRP
Sbjct: 438 LQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRP 497

Query: 512 WKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYYAEFNNQGPGAKTDARVKWPGYKV 571
           WKEFSRTI+M +TI+D+I P GW+PW GDFAL TLYYAE+ N GPG+    RVKWPG K 
Sbjct: 498 WKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKK 557

Query: 572 IDKDEAAKFTIGTFLELD-WIESASVP 588
           +   +A +FT   FL  + WI    VP
Sbjct: 558 LSPKQALRFTPARFLRGNLWIPPNRVP 565

BLAST of Lag0042190.1 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 461.8 bits (1187), Expect = 8.1e-130
Identity = 246/582 (42.27%), Postives = 356/582 (61.17%), Query Frame = 0

Query: 16  ERARKFRQRVTIAVISVSVILLVVVAAVFVQVNSNSSKSSSKDSDGNSKDPDTPETKQVS 75
           + +++ R+ + I + SV +I +VV   V V +N +         DG+SK        +V+
Sbjct: 7   DESKRKRRYIVITISSVLLISMVVAVTVGVSLNKH---------DGDSKG-----KAEVN 66

Query: 76  RVDKMIVMICNSTDYKGKCETTLKDGVKTDPDSSDPKDLIKLAISAAATELKSAVKKAEG 135
              K +  +C  TDY+  CE TL   +K   +++DP +L+K A +    ++  A KK++ 
Sbjct: 67  ASVKAVKDVCAPTDYRKTCEDTL---IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQT 126

Query: 136 FNFATAEEKG--AFEDCKVLFEDAVEELEISVSEMSRKNLGKLTAKTTPDLNNWLSAVIS 195
                 + +   A + CK L + A++EL  S  E+ +     L  +   +L  WLSA IS
Sbjct: 127 IMELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEF-HLLDEALINLRIWLSAAIS 186

Query: 196 YQETCVDGF--PDGKLKSDMEKAVKSVKELTSNSLAMISQVVSFFSTFEKPDGEEAATRR 255
           ++ETC++GF    G     M+KA+K+  ELT N LA+IS++ +F    + P        R
Sbjct: 187 HEETCLEGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIP----GLNSR 246

Query: 256 RLLARDGLPIWMNTQERRMLKGATAGDKPKPNVVVAKDGSGNFKTINEALAAMPAKYDGR 315
           RLLA +G P W++ + R++L+ A A    KP++VVA+DGSG +KTINEAL  +P K +  
Sbjct: 247 RLLA-EGFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTT 306

Query: 316 YVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGKKNFVDGVRTFQTATFVALGDGF 375
           +V+++K G+Y+E V + K M+++   GDG  K++ISG KN+ DG+ T++TAT   +G+ F
Sbjct: 307 FVVHIKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYF 366

Query: 376 LGQAIGFRNTAGPEKHQAVAARIQGDRAIFVNCRFDGYQDTLYAQAHRQYYRSCLITGTI 435
           + + IGF NTAG  KHQAVA R+Q D +IF NCRFDGYQDTLY  +HRQ++R C I+GTI
Sbjct: 367 IAKNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTI 426

Query: 436 DFIFGDAAAIFQNCNLMVRKPLDKQQNIVTAQGRTDKHETTGIVLQNCKILPDKTLEPVK 495
           DF+FGDAAA+FQNC L+VRKPL  Q   +TA GR D  E+TG V Q C I  +     VK
Sbjct: 427 DFLFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVK 486

Query: 496 ANFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYYAEFNNQGPGAKTD 555
              K+YLGRPWKE+SRTI+M + I D + P GW PW GDF LKTL+Y+E  N GPG+   
Sbjct: 487 ETSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALA 546

Query: 556 ARVKWPGYKVIDKDEAAKFTIGTFLE-LDWIESASVPVHAGL 593
            RV W G K + +++  KFT   +++  DWI    VP   GL
Sbjct: 547 NRVTWAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of Lag0042190.1 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 459.1 bits (1180), Expect = 5.2e-129
Identity = 246/583 (42.20%), Postives = 355/583 (60.89%), Query Frame = 0

Query: 16  ERARKFRQRVTIAVISVSVILLVVVAAVFVQVNSNSSKSSSKDSDGNSKDPDTPETKQVS 75
           E A++ ++ V I++ SV +I +VV   + V VN +                D    ++++
Sbjct: 8   EDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKS----------------DNAGDEEIT 67

Query: 76  RVDKMIVMICNSTDYKGKCETTLKDGVKTDPDSSDPKDLIKLAISAAATELKSAVKKAEG 135
              K I  +C  TDYK  CE TL+   K   D+SDP +L+K A +A   ++    KK++ 
Sbjct: 68  TSVKAIKDVCAPTDYKETCEDTLRKDAK---DTSDPLELVKTAFNATMKQISDVAKKSQT 127

Query: 136 FNFATAE--EKGAFEDCKVLFEDAVEELEISVSEMSRKNLGKLTAKTTPDLNNWLSAVIS 195
                 +   K A + CK L + A+ EL  S  E+ +    K+  +    L  WLSA IS
Sbjct: 128 MIELQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVD-EALVKLRIWLSATIS 187

Query: 196 YQETCVDGF--PDGKLKSDMEKAVKSVKELTSNSLAMISQVVSFFSTFEKPDGEEAATRR 255
           +++TC+DGF    G     ++KA+K+  +LT N LAM++++ ++    + P+       R
Sbjct: 188 HEQTCLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPE----MNSR 247

Query: 256 RLLARDGLPIWMNTQERRMLKGATAGDKPKPNVVVAKDGSGNFKTINEALAAMPAKYDGR 315
           RLL+++  P WM+ + RR+L    +  + KP++VVA+DGSG +KTINEAL  +P K +  
Sbjct: 248 RLLSQE-FPSWMDARARRLLNAPMS--EVKPDIVVAQDGSGQYKTINEALNFVPKKKNTT 307

Query: 316 YVIYVKEGIYEETVVITKKMANVTMYGDGSQKSMISGKKNFVDGVRTFQTATFVALGDGF 375
           +V+++KEGIY+E V + + M ++   GDG  K++ISG K++ DG+ T++TAT   +GD F
Sbjct: 308 FVVHIKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHF 367

Query: 376 LGQAIGFRNTAGPEKHQAVAARIQGDRAIFVNCRFDGYQDTLYAQAHRQYYRSCLITGTI 435
           + + I F NTAG  KHQAVA R+  D +IF NC+FDGYQDTLYA +HRQ+YR C I+GTI
Sbjct: 368 IAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTI 427

Query: 436 DFIFGDAAAIFQNCNLMVRKPLDKQQNIVTAQGRTDKHETTGIVLQNCKILPDKTLEPVK 495
           DF+FGDAAA+FQNC L+VRKPL  Q   +TA GR D  E+TG VLQ C I+ +     VK
Sbjct: 428 DFLFGDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVK 487

Query: 496 ANFKSYLGRPWKEFSRTIVMESTIEDIIHPDGWMPWEGDFALKTLYYAEFNNQGPGAKTD 555
              K+YLGRPWKE+SRTI+M + I D + P+GW PW G+F L TL+Y+E  N GPGA   
Sbjct: 488 EQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAIT 547

Query: 556 ARVKWPGYKVIDKDEAAKFTIGTFLELD-WIESASVPVHAGLF 594
            RV WPG K +  +E  KFT   +++ D WI    VP   GLF
Sbjct: 548 KRVTWPGIKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGLF 563