Lag0032543.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0032543.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	CCHC-type domain-containing protein
Location	chr11: 34349099 .. 34349783 (-)
Sequence length	615
RNA-Seq Expression	Lag0032543.1
Synteny	Lag0032543.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAACATCTTACGAAATACTTCCAGAGTTTTGTTATAGATGTGGTAGGGTGGGTCATGTGATAAGAGAATGCGAAGAAATAAACAATGGGCCAGAAGAAAACAATATTCAGGAAGAACTTCTATTCGGAGACTGGATGAAAGTGACTCCTCTATTTGAAGGAACCGCGCCATCGGGGTTTCCAAGTGTTAAGAATGACTTCAGATGGGCAAGGGGAGGAGACCGAGGAAAAGGAAGACAGAGGAAAAGGAAGACAGAACCCCCATCAGACCAGCGGTAGTTAGGCTATCGAAAGAAATTCTAAAAATAAACAATCTGGAAACTGGTTTCACCGAAAGGAAAGAAGGGAGGCCAGAGGTCCGGCCAACAGACTACTGGAAACGGAGAAGACAGAGAGGGCAAATCCAAACCTAAAAAAAGTCACATCGGTGCCAACGACTAGTTCTCAACCTGAAAAGGAAACTCCTGGGAAAGCGACAGAGAGGACTTGTCTTCAGAAAGGAAAAGGAATACCAGGGGAAGTGTCAGAGAGGATCAGTGAAAGTGCTCAGAGGATGGATACAGAGAACGAGAAATCCAAAGAGACAACTATCAAGGACAAAGGAAAAGCAGTGTGGGAAAACAGAAAAGGACAAGAAAAAGGGACTATGGTCAACGAACTACTAAAGCAAAAGAAAGCTTAG

mRNA sequence

ATGGGAACATCTTACGAAATACTTCCAGAGTTTTGTTATAGATGTGGTAGGGTGGGTCATGTGATAAGAGAATGCGAAGAAATAAACAATGGGCCAGAAGAAAACAATATTCAGGAAGAACTTCTATTCGGAGACTGGATGAAAGTGACTCCTCTATTTGAAGGAACCGCGCCATCGGGGTTTCCAAGTGTTAAGAATGACTTCAGATGGGCAAGGGGAGGAGACCGAGGAAAAGGAAGACAGAGGAAAAGGAAGACAGAACCCCCATCAGACCAGCGGGAGGCCAGAGGTCCGGCCAACAGACTACTGGAAACGGAGAAGACAGAGAGGGCAAATCCAAACCTAAAAAAAGTCACATCGGTGCCAACGACTAGTTCTCAACCTGAAAAGGAAACTCCTGGGAAAGCGACAGAGAGGACTTGTCTTCAGAAAGGAAAAGGAATACCAGGGGAAGTGTCAGAGAGGATCAGTGAAAGTGCTCAGAGGATGGATACAGAGAACGAGAAATCCAAAGAGACAACTATCAAGGACAAAGGAAAAGCAGTGTGGGAAAACAGAAAAGGACAAGAAAAAGGGACTATGGTCAACGAACTACTAAAGCAAAAGAAAGCTTAG

Coding sequence (CDS)

ATGGGAACATCTTACGAAATACTTCCAGAGTTTTGTTATAGATGTGGTAGGGTGGGTCATGTGATAAGAGAATGCGAAGAAATAAACAATGGGCCAGAAGAAAACAATATTCAGGAAGAACTTCTATTCGGAGACTGGATGAAAGTGACTCCTCTATTTGAAGGAACCGCGCCATCGGGGTTTCCAAGTGTTAAGAATGACTTCAGATGGGCAAGGGGAGGAGACCGAGGAAAAGGAAGACAGAGGAAAAGGAAGACAGAACCCCCATCAGACCAGCGGGAGGCCAGAGGTCCGGCCAACAGACTACTGGAAACGGAGAAGACAGAGAGGGCAAATCCAAACCTAAAAAAAGTCACATCGGTGCCAACGACTAGTTCTCAACCTGAAAAGGAAACTCCTGGGAAAGCGACAGAGAGGACTTGTCTTCAGAAAGGAAAAGGAATACCAGGGGAAGTGTCAGAGAGGATCAGTGAAAGTGCTCAGAGGATGGATACAGAGAACGAGAAATCCAAAGAGACAACTATCAAGGACAAAGGAAAAGCAGTGTGGGAAAACAGAAAAGGACAAGAAAAAGGGACTATGGTCAACGAACTACTAAAGCAAAAGAAAGCTTAG

Protein sequence

MGTSYEILPEFCYRCGRVGHVIRECEEINNGPEENNIQEELLFGDWMKVTPLFEGTAPSGFPSVKNDFRWARGGDRGKGRQRKRKTEPPSDQREARGPANRLLETEKTERANPNLKKVTSVPTTSSQPEKETPGKATERTCLQKGKGIPGEVSERISESAQRMDTENEKSKETTIKDKGKAVWENRKGQEKGTMVNELLKQKKA

Homology

[Close]

BLAST of Lag0032543.1 vs. NCBI nr
Match: KAA0046998.1 (reverse transcriptase [Cucumis melo var. makuwa] >TYK04784.1 reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 63.9 bits (154), Expect = 1.8e-06
Identity = 31/88 (35.23%), Postives = 49/88 (55.68%), Query Frame = 0


Query: 4   SYEILPEFCYRCGRVGHVIRECEEINNGPEENNIQEELLFGDWMKVTPLFEGTAPSGFPS 63
           SYE LP+FCY+CGRVGHV++EC+E+ +  EE        FG W++ +   +  +      
Sbjct: 58  SYEKLPDFCYKCGRVGHVVQECKEVGDDGEEGQ-----RFGVWLRKSQTSQSISKGRREE 117

Query: 64  VKNDFRWARGGDRGKGRQRKRKTEPPSD 92
           V++ F   RG   GKG +  ++ +   D
Sbjct: 118 VRSQFTIDRGRGEGKGIEAFKRDKSSRD 140

[Close]

BLAST of Lag0032543.1 vs. NCBI nr
Match: GAU49029.1 (hypothetical protein TSUD_406820 [Trifolium subterraneum])

HSP 1 Score: 63.2 bits (152), Expect = 3.1e-06
Identity = 27/53 (50.94%), Postives = 37/53 (69.81%), Query Frame = 0


Query: 5   YEILPEFCYRCGRVGHVIRECEEINNGPEEN--NIQE-ELLFGDWMKVTPLFE 55
           YE LP FCY CGR+GH IR+C+E+     EN  +I+E EL +G WM+ +PL +
Sbjct: 154 YECLPNFCYVCGRIGHQIRDCDEVGEKDGENYEDIEESELAYGGWMRASPLLK 206

[Close]

BLAST of Lag0032543.1 vs. NCBI nr
Match: PNY15174.1 (ribonuclease H, partial [Trifolium pratense])

HSP 1 Score: 61.2 bits (147), Expect = 1.2e-05
Identity = 24/51 (47.06%), Postives = 35/51 (68.63%), Query Frame = 0


Query: 5   YEILPEFCYRCGRVGHVIRECEEINNGPE---ENNIQEELLFGDWMKVTPL 53
           YE LP FCY CGR+GH +++CEE+    E   E+  ++EL +G W+K +PL
Sbjct: 280 YERLPNFCYMCGRIGHQMKDCEEVEGNEEDGYEDIEEKELPYGSWLKASPL 330

[Close]

BLAST of Lag0032543.1 vs. NCBI nr
Match: MCI38195.1 (hypothetical protein [Trifolium medium])

HSP 1 Score: 60.8 bits (146), Expect = 1.5e-05
Identity = 26/51 (50.98%), Postives = 37/51 (72.55%), Query Frame = 0


Query: 5  YEILPEFCYRCGRVGHVIRECEEINNGPEE--NNIQE-ELLFGDWMKVTPL 53
          YE LP FCY CGR+GH I++CEE+    +E  ++I+E EL +G W+K +PL
Sbjct: 15 YERLPTFCYVCGRIGHQIKDCEEVEGKDDEGYDDIEEKELPYGSWLKASPL 65

[Close]

BLAST of Lag0032543.1 vs. NCBI nr
Match: TXG48918.1 (hypothetical protein EZV62_024793 [Acer yangbiense])

HSP 1 Score: 60.5 bits (145), Expect = 2.0e-05
Identity = 38/93 (40.86%), Postives = 47/93 (50.54%), Query Frame = 0


Query: 5   YEILPEFCYRCGRVGHVIREC--EEINNGPEENNIQEELLFGDWMKVTPLFEGTAPSGFP 64
           YE LPE CYRCGR+GHV+R C  + +   PE+ N    LLFG WMKV            P
Sbjct: 152 YEKLPEHCYRCGRLGHVVRNCPVKVVGEEPEDFN----LLFGPWMKVES----------P 211

Query: 65  SVKNDFR---------WARGGDRGKGRQRKRKT 87
           + +NDFR          + GG  G G+    KT
Sbjct: 212 AKRNDFRQRYEENIYGGSGGGGSGSGKPNFGKT 230

[Close]

BLAST of Lag0032543.1 vs. ExPASy TrEMBL
Match: A0A5D3C0K2 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G001040 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 8.7e-07
Identity = 31/88 (35.23%), Postives = 49/88 (55.68%), Query Frame = 0


Query: 4   SYEILPEFCYRCGRVGHVIRECEEINNGPEENNIQEELLFGDWMKVTPLFEGTAPSGFPS 63
           SYE LP+FCY+CGRVGHV++EC+E+ +  EE        FG W++ +   +  +      
Sbjct: 58  SYEKLPDFCYKCGRVGHVVQECKEVGDDGEEGQ-----RFGVWLRKSQTSQSISKGRREE 117

Query: 64  VKNDFRWARGGDRGKGRQRKRKTEPPSD 92
           V++ F   RG   GKG +  ++ +   D
Sbjct: 118 VRSQFTIDRGRGEGKGIEAFKRDKSSRD 140

[Close]

BLAST of Lag0032543.1 vs. ExPASy TrEMBL
Match: A0A2Z6PPZ2 (CCHC-type domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TSUD_406820 PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.5e-06
Identity = 27/53 (50.94%), Postives = 37/53 (69.81%), Query Frame = 0


Query: 5   YEILPEFCYRCGRVGHVIRECEEINNGPEEN--NIQE-ELLFGDWMKVTPLFE 55
           YE LP FCY CGR+GH IR+C+E+     EN  +I+E EL +G WM+ +PL +
Sbjct: 154 YECLPNFCYVCGRIGHQIRDCDEVGEKDGENYEDIEESELAYGGWMRASPLLK 206

[Close]

BLAST of Lag0032543.1 vs. ExPASy TrEMBL
Match: A0A2K3PIT6 (Ribonuclease H (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g011865 PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 5.7e-06
Identity = 24/51 (47.06%), Postives = 35/51 (68.63%), Query Frame = 0


Query: 5   YEILPEFCYRCGRVGHVIRECEEINNGPE---ENNIQEELLFGDWMKVTPL 53
           YE LP FCY CGR+GH +++CEE+    E   E+  ++EL +G W+K +PL
Sbjct: 280 YERLPNFCYMCGRIGHQMKDCEEVEGNEEDGYEDIEEKELPYGSWLKASPL 330

[Close]

BLAST of Lag0032543.1 vs. ExPASy TrEMBL
Match: A0A392RRI2 (CCHC-type domain-containing protein (Fragment) OS=Trifolium medium OX=97028 PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 7.4e-06
Identity = 26/51 (50.98%), Postives = 37/51 (72.55%), Query Frame = 0


Query: 5  YEILPEFCYRCGRVGHVIRECEEINNGPEE--NNIQE-ELLFGDWMKVTPL 53
          YE LP FCY CGR+GH I++CEE+    +E  ++I+E EL +G W+K +PL
Sbjct: 15 YERLPTFCYVCGRIGHQIKDCEEVEGKDDEGYDDIEEKELPYGSWLKASPL 65

[Close]

BLAST of Lag0032543.1 vs. ExPASy TrEMBL
Match: A0A5C7GW15 (CCHC-type domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_024793 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 9.7e-06
Identity = 38/93 (40.86%), Postives = 47/93 (50.54%), Query Frame = 0


Query: 5   YEILPEFCYRCGRVGHVIREC--EEINNGPEENNIQEELLFGDWMKVTPLFEGTAPSGFP 64
           YE LPE CYRCGR+GHV+R C  + +   PE+ N    LLFG WMKV            P
Sbjct: 152 YEKLPEHCYRCGRLGHVVRNCPVKVVGEEPEDFN----LLFGPWMKVES----------P 211

Query: 65  SVKNDFR---------WARGGDRGKGRQRKRKT 87
           + +NDFR          + GG  G G+    KT
Sbjct: 212 AKRNDFRQRYEENIYGGSGGGGSGSGKPNFGKT 230

The following BLAST results are available for this feature:

BLAST of Lag0032543.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0046998.1	1.8e-06	35.23	reverse transcriptase [Cucumis melo var. makuwa] >TYK04784.1 reverse transcripta...	[more]
GAU49029.1	3.1e-06	50.94	hypothetical protein TSUD_406820 [Trifolium subterraneum]	[more]
PNY15174.1	1.2e-05	47.06	ribonuclease H, partial [Trifolium pratense]	[more]
MCI38195.1	1.5e-05	50.98	hypothetical protein [Trifolium medium]	[more]
TXG48918.1	2.0e-05	40.86	hypothetical protein EZV62_024793 [Acer yangbiense]	[more]

BLAST of Lag0032543.1 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Lag0032543.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C0K2	8.7e-07	35.23	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68...	[more]
A0A2Z6PPZ2	1.5e-06	50.94	CCHC-type domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TSUD_40...	[more]
A0A2K3PIT6	5.7e-06	47.06	Ribonuclease H (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g011865 PE=4 SV...	[more]
A0A392RRI2	7.4e-06	50.98	CCHC-type domain-containing protein (Fragment) OS=Trifolium medium OX=97028 PE=4...	[more]
A0A5C7GW15	9.7e-06	40.86	CCHC-type domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_02479...	[more]

BLAST of Lag0032543.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001878	Zinc finger, CCHC-type	SMART	SM00343	c2hcfinal6	coord: 11..27 e-value: 0.0089 score: 23.9
IPR001878	Zinc finger, CCHC-type	PFAM	PF00098	zf-CCHC	coord: 12..25 e-value: 7.4E-5 score: 22.7
IPR001878	Zinc finger, CCHC-type	PROSITE	PS50158	ZF_CCHC	coord: 12..27 score: 10.377962
None	No IPR available	GENE3D	4.10.60.10		coord: 10..158 e-value: 2.4E-5 score: 26.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 114..131
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 157..189
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 69..113
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 55..189
IPR036875	Zinc finger, CCHC-type superfamily	SUPERFAMILY	57756	Retrovirus zinc finger-like domains	coord: 10..33

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0032543	Lag0032543	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0032543.1.exon2	Lag0032543.1.exon2	exon
Lag0032543.1.exon1	Lag0032543.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0032543.1	cds.Lag0032543.1_2	CDS
cds.Lag0032543.1	cds.Lag0032543.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0032543.1	Lag0032543.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090304	nucleic acid metabolic process
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0140640	catalytic activity, acting on a nucleic acid