Lag0031255.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0031255.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Ribonuclease 3-like protein 1
Location	chr11: 6344969 .. 6347949 (-)
Sequence length	597
RNA-Seq Expression	Lag0031255.1
Synteny	Lag0031255.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAATACTCCTTCTTCATACCCTCGAAAATTAAATGTCGATCTCAAGAATCTTCCTCCAATCAATAATCTGCGCATTGGAAATGGAAATCGCTCCTCAATTCCGGTAAAACTCGGTGGTTTTCCCGCGACTGTGTTCGATTTTCTTTGGAATTCGAAAGGAATTTGATGTTCGAGTTGAAAATTTTCAGGTTAAATTTGAGAAATCCAGGTATATTAGGCGCGTTCCTCAGTTTATGCCCCCGAATCACCGGCCGGAAATGAAGCCCAACGAATACTCTGATTCCGCTCGAGCTCTGAATACGAAGACGGTTCCAGAGGCTTCGTCGGATTCCTTCGATAAAAAGAAAGAGAATAGCCGGGAGATTGCTTCGACTTGTAGTTGCGGACATGGCTCGACCAAAGAAGGTACCGATTCAAACCTAGTTCCTAAACTTGATTGCTGTTTGGATTTCGAAATAGATGATTCCTGTGATTTTTCATCATTTTCATGGATTATTCTTTTCCTTTTCTTCGTTATTTGCTGGTTTTCTTCTAATTGATTGATTTGAAAATGCAATTGAATGTTCATCTCACTTGTATTCCATCAGGAGAAGTCTTTATCGCCCATTTCTCCCCAATCCCTCTCCCACTTCGTCGTCATTAGCATTCGCCAGTAATTTTAACGTGCAGTTACAAACCCAAAAAAGAACAAAGAAAAAAGAAAAAAACAATAAAGGGTTTTCTATGGTAAGTATTTGATCTCAACACCTCCAAAACTGAACTCTGAATTATTTCCCTGATTGTGTTTGAAGATCATCATTACTTCCTTTCTTATTAGAGAGCGGGATGGATGTTATCATCTATATCATTTTTTTTCTTGTTTGTCTTCAATTCTTCTTAGCTGCCTTGTTTTCTAAAAGAAATGAAGTTGTACGTGAATTTTGTATTCCAACTATACAATTAATTTCAACAATAACAGTAACATTATAAATTATCCAATAGAAATCTTTAGTGGCTGAAAGTTGTGAGTATAGTTTTCTAAAGTAGGCCATTAGTTTCTTAAAATAACAAGTTACTCGTTAACCCTCAAGGGGTGACCTGCCCGTAAGAGCTGGAATCTCAAAATCCCACTTCAAATATGTAAATAGAAAACTCACGCCGAGTATGATAACCATTTGGTTTTTGAAAATTATGTTTGTTTTCTTTCAATTTTCTTCCTATGGTTTAAATTCTTAGTCAAATTCCAATAACAAAAACAAGTTTTTTGAAAACTACGGTCCCGTTTGATAACCATTTCATTTTTGGTTTTTGGTTTTTGAAAGTCAAGCCTACAAACACCACTTTTATTCACAAGTTTCTTTGTTTTGTTATCTACAACTTTTCCTTGTTTTCAAAAACCAAGTCAAATTTTGAAAACAAAAAAAAAGTAGTTTTCAAAAACTTGTTTTTGTTTTTAGAATTTGACTTGGAATTCAAATGTGTCGTTGAGAAAGATGAAAACCATTGTAAGGAAATTGGGAAGAAACAAGCATAATTTTCAAAAATCAAAACCAAATAGTTCTCAAACGAGACCTACTTTTTTTAGTTTCAAAACTTGACTTGTTTTTAAAACATGGGAAGAATGTAGATAACAAAACAAAGAAATATAAGTGAAAGTATGGTTTATAAACTTAATTTTTAAACGGAAAATCAAATAGTTATCCAATCTTCCTTAATATTTAAAATCTTTGTTGTCTTTCTCAAAAAAAAAAAAAAAAAAAAAATCTTTGTTGTCTTTTAGAGTTGACTTTGGAGCTGAGCTCCTTGGAGACTAATAGAGTGGGTCCCATTCTCTAGTTACCAATTAAAAGAAAAAACTCAGGAATAAAGACAGACAAAACTAACTTAGTAATCAGGTACTCAAACATGTTTATACCCTTTTTTGTTTGAAAATCGAAAGTGATTTTGAAACTCAACTAGCAAAAAGCACATAGGCTCTTGCACTTTGATGGCTTACTCGAGTATTATCGACACTTCATTTCATTTAACTTGCCTTCCTTATCTCAGCTTGAGTACATTGGCCTTTTCAATTATACTGCTTATTCTGATTCCCATAAAATTTACCATGGCGTTTCAAAAGCACTGTAAATGCTCAGAAATTTCCATTTTGGGTATTCAAAGTTTGCTTTAATGGTATAACTTCACCTTTCCATTTTAGGTAATTCAGAAAAGAGAGCGGCAAAGTCCCTTCTGTTTGAGATTTGCACTGCAAATCACTGGCAGCCTCCCCTGTTTGAATGCTGCGAGGAAGAAGGTCCAAGCCACGTGAAAAAGTAAGCTCAATGACTAATCATCAATATGAGTAAGCGTTAGTAGTTATGAAGGACTAACCCTTAATTCGAGTAACATATAGTGCCAATCTTTCAACACCCTCACAAAAGTGAAGGTATATTTTGGAGTGATTTTGAACCTGACAAAAGTGATTTTAATTATTTCAAAATAACTTATGAACATGTTCTTGGTTCTTGTCTTGTGGATTACTCGCTAAAACTCGAAAAATCTCCATTCTAACACCTACTTTTTGAAGCTTTTCTAACGTTTCTGCCTTTTACCTTCAAAATGGGGATAACTAGATGTCACTATGGATGTTTTTGAGAAGATGAAAGGTTACCAAGAGAGAGCTGTTCAAGTTTCTTTTTAGCTTCAATTTCCTCATTGTTTGGATTTGACATGTTCATAGCCATCTGTGTGCAAACATGAGCTAGAGCATAGATTTGAGAGTATTGATTTCCAAACAGGATTCAATAGAAGCGTTATTTAATGGGATTTTTGCAACTGATGTAAATTTGATTTTGATTTAGGTACAGGTTCAAGGTTATTGTTGAGATGAAGGGAGCTTCTGAAGCAGTTTTGGAATGCTATGGGAACCCTCAAACAAGAAAGAAAGTAGCAGCAGAGCATGCAGCAGAAGGAGCATTATGGTATTTAAAGCATTTGGGATATCGTTCTAAAAGATAA

mRNA sequence

ATGGAGAATACTCCTTCTTCATACCCTCGAAAATTAAATGTCGATCTCAAGAATCTTCCTCCAATCAATAATCTGCGCATTGGAAATGGAAATCGCTCCTCAATTCCGGTTAAATTTGAGAAATCCAGGTATATTAGGCGCGTTCCTCAGTTTATGCCCCCGAATCACCGGCCGGAAATGAAGCCCAACGAATACTCTGATTCCGCTCGAGCTCTGAATACGAAGACGGTTCCAGAGGCTTCGTCGGATTCCTTCGATAAAAAGAAAGAGAATAGCCGGGAGATTGCTTCGACTTGTAGTTGCGGACATGGCTCGACCAAAGAAGGTAATTCAGAAAAGAGAGCGGCAAAGTCCCTTCTGTTTGAGATTTGCACTGCAAATCACTGGCAGCCTCCCCTGTTTGAATGCTGCGAGGAAGAAGGTCCAAGCCACGTGAAAAAGTACAGGTTCAAGGTTATTGTTGAGATGAAGGGAGCTTCTGAAGCAGTTTTGGAATGCTATGGGAACCCTCAAACAAGAAAGAAAGTAGCAGCAGAGCATGCAGCAGAAGGAGCATTATGGTATTTAAAGCATTTGGGATATCGTTCTAAAAGATAA

Coding sequence (CDS)

ATGGAGAATACTCCTTCTTCATACCCTCGAAAATTAAATGTCGATCTCAAGAATCTTCCTCCAATCAATAATCTGCGCATTGGAAATGGAAATCGCTCCTCAATTCCGGTTAAATTTGAGAAATCCAGGTATATTAGGCGCGTTCCTCAGTTTATGCCCCCGAATCACCGGCCGGAAATGAAGCCCAACGAATACTCTGATTCCGCTCGAGCTCTGAATACGAAGACGGTTCCAGAGGCTTCGTCGGATTCCTTCGATAAAAAGAAAGAGAATAGCCGGGAGATTGCTTCGACTTGTAGTTGCGGACATGGCTCGACCAAAGAAGGTAATTCAGAAAAGAGAGCGGCAAAGTCCCTTCTGTTTGAGATTTGCACTGCAAATCACTGGCAGCCTCCCCTGTTTGAATGCTGCGAGGAAGAAGGTCCAAGCCACGTGAAAAAGTACAGGTTCAAGGTTATTGTTGAGATGAAGGGAGCTTCTGAAGCAGTTTTGGAATGCTATGGGAACCCTCAAACAAGAAAGAAAGTAGCAGCAGAGCATGCAGCAGAAGGAGCATTATGGTATTTAAAGCATTTGGGATATCGTTCTAAAAGATAA

Protein sequence

MENTPSSYPRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPEMKPNEYSDSARALNTKTVPEASSDSFDKKKENSREIASTCSCGHGSTKEGNSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGNPQTRKKVAAEHAAEGALWYLKHLGYRSKR

Homology

[Close]

BLAST of Lag0031255.1 vs. NCBI nr
Match: XP_038878793.1 (ribonuclease 3-like protein 1 isoform X3 [Benincasa hispida])

HSP 1 Score: 303.5 bits (776), Expect = 1.3e-78
Identity = 160/205 (78.05%), Postives = 170/205 (82.93%), Query Frame = 0


Query: 1   MENTPSSYPRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPE- 60
           MENT  S PR+LN +LKNLPPINN  IG GN SSI  KFEKSRYIRRVPQF+P NHRP  
Sbjct: 1   MENT-HSCPRRLNFNLKNLPPINNSLIGTGNHSSISAKFEKSRYIRRVPQFIPQNHRPNE 60

Query: 61  -----MKPNEYSDSARALNTKTVPEASSDSFDKKKENSREIASTCS-CGHGSTKEGNSEK 120
                MKP   S SARALN+KT+PEA SDSF +KK+NS+EIAS  S CGHGSTKEG  EK
Sbjct: 61  YAGLPMKP-ACSGSARALNSKTIPEAPSDSFVQKKDNSQEIASRSSCCGHGSTKEGTPEK 120

Query: 121 RAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGNPQTR 180
           RAAKSLLFEICTANHWQPPLFECCEEEGPSH KKYRFKV VEMKGASE VLECYGN QTR
Sbjct: 121 RAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVTVEMKGASETVLECYGNLQTR 180

Query: 181 KKVAAEHAAEGALWYLKHLGYRSKR 199
           KKVAAEHAAEGA+WYLK+LGY  KR
Sbjct: 181 KKVAAEHAAEGAIWYLKNLGYCFKR 203

[Close]

BLAST of Lag0031255.1 vs. NCBI nr
Match: XP_038878769.1 (ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida] >XP_038878776.1 ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 303.5 bits (776), Expect = 1.3e-78
Identity = 160/205 (78.05%), Postives = 170/205 (82.93%), Query Frame = 0


Query: 1   MENTPSSYPRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPE- 60
           MENT  S PR+LN +LKNLPPINN  IG GN SSI  KFEKSRYIRRVPQF+P NHRP  
Sbjct: 18  MENT-HSCPRRLNFNLKNLPPINNSLIGTGNHSSISAKFEKSRYIRRVPQFIPQNHRPNE 77

Query: 61  -----MKPNEYSDSARALNTKTVPEASSDSFDKKKENSREIASTCS-CGHGSTKEGNSEK 120
                MKP   S SARALN+KT+PEA SDSF +KK+NS+EIAS  S CGHGSTKEG  EK
Sbjct: 78  YAGLPMKP-ACSGSARALNSKTIPEAPSDSFVQKKDNSQEIASRSSCCGHGSTKEGTPEK 137

Query: 121 RAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGNPQTR 180
           RAAKSLLFEICTANHWQPPLFECCEEEGPSH KKYRFKV VEMKGASE VLECYGN QTR
Sbjct: 138 RAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVTVEMKGASETVLECYGNLQTR 197

Query: 181 KKVAAEHAAEGALWYLKHLGYRSKR 199
           KKVAAEHAAEGA+WYLK+LGY  KR
Sbjct: 198 KKVAAEHAAEGAIWYLKNLGYCFKR 220

[Close]

BLAST of Lag0031255.1 vs. NCBI nr
Match: XP_022153429.1 (uncharacterized protein LOC111020939 isoform X1 [Momordica charantia])

HSP 1 Score: 302.8 bits (774), Expect = 2.2e-78
Identity = 155/195 (79.49%), Postives = 166/195 (85.13%), Query Frame = 0


Query: 1   MENTPSSYPRKLNVDLKNLPPINNLRIGNGNRS-SIPVKFEKSRYIRRVPQFMPPNHRPE 60
           ME TP  YPR+LNV+LKNLPPIN  R GNGN   SIP KFEKSRYIRRVP F PPNHRPE
Sbjct: 1   MEQTP-PYPRRLNVNLKNLPPINP-RTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPE 60

Query: 61  MKPNEYSDSARALNTKTVPEASSDSFDKKKENSREIASTCSCGHGSTKEGNSEKRAAKSL 120
           MKP    D+A ALNTKT+PEASSDSF  K ENSREIASTCSCGH S KEG  EKRAAKSL
Sbjct: 61  MKP----DTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSL 120

Query: 121 LFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGNPQTRKKVAAE 180
           LFE+CTAN+W+PPLFECCEEEGPSH KK+RFKVIVEMKGA +AVLECYGN Q RKKVAA+
Sbjct: 121 LFEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLECYGNSQARKKVAAQ 180

Query: 181 HAAEGALWYLKHLGY 195
           +AAEGALW LKHLGY
Sbjct: 181 NAAEGALWCLKHLGY 189

[Close]

BLAST of Lag0031255.1 vs. NCBI nr
Match: XP_004152191.1 (uncharacterized protein LOC101212514 [Cucumis sativus] >KGN52916.1 hypothetical protein Csa_014441 [Cucumis sativus])

HSP 1 Score: 299.3 bits (765), Expect = 2.5e-77
Identity = 153/209 (73.21%), Postives = 170/209 (81.34%), Query Frame = 0


Query: 1   MENTPSSYPRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPEM 60
           MEN PS  P +LN++LKNLPPINN R  +G+ SSIP KFEKSRYIRRVPQFMP +HRPE+
Sbjct: 19  MENNPSCPPPRLNINLKNLPPINNPRNVSGSHSSIPAKFEKSRYIRRVPQFMPSDHRPEI 78

Query: 61  KPNE---------YSDSARALNTKTVPEA-SSDSFDKKKENSREIASTCS-CGHGSTKEG 120
           + N+         YSDSARALNTKT P A S+DSF +KK+  +++AS CS C H STKEG
Sbjct: 79  RSNDYAGLPMKLAYSDSARALNTKTFPVAPSADSFVRKKDTCKDLASRCSCCRHDSTKEG 138

Query: 121 NSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGN 180
             EKRAAKSLLFEICTANHWQPPLFECCEEEGPSH KKYRFKV +EMKG  EAV+ECYGN
Sbjct: 139 TPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIEMKGDCEAVVECYGN 198

Query: 181 PQTRKKVAAEHAAEGALWYLKHLGYRSKR 199
            QTRKKVAAEHAAEGALWYL HLGYR KR
Sbjct: 199 LQTRKKVAAEHAAEGALWYLNHLGYRFKR 227

[Close]

BLAST of Lag0031255.1 vs. NCBI nr
Match: XP_038878786.1 (ribonuclease 3-like protein 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 288.5 bits (737), Expect = 4.4e-74
Identity = 152/194 (78.35%), Postives = 161/194 (82.99%), Query Frame = 0


Query: 1   MENTPSSYPRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPE- 60
           MENT  S PR+LN +LKNLPPINN  IG GN SSI  KFEKSRYIRRVPQF+P NHRP  
Sbjct: 18  MENT-HSCPRRLNFNLKNLPPINNSLIGTGNHSSISAKFEKSRYIRRVPQFIPQNHRPNE 77

Query: 61  -----MKPNEYSDSARALNTKTVPEASSDSFDKKKENSREIASTCS-CGHGSTKEGNSEK 120
                MKP   S SARALN+KT+PEA SDSF +KK+NS+EIAS  S CGHGSTKEG  EK
Sbjct: 78  YAGLPMKP-ACSGSARALNSKTIPEAPSDSFVQKKDNSQEIASRSSCCGHGSTKEGTPEK 137

Query: 121 RAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGNPQTR 180
           RAAKSLLFEICTANHWQPPLFECCEEEGPSH KKYRFKV VEMKGASE VLECYGN QTR
Sbjct: 138 RAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVTVEMKGASETVLECYGNLQTR 197

Query: 181 KKVAAEHAAEGALW 188
           KKVAAEHAAEGA+W
Sbjct: 198 KKVAAEHAAEGAIW 209

[Close]

BLAST of Lag0031255.1 vs. ExPASy Swiss-Prot
Match: A7LFZ6 (Endoribonuclease Dicer homolog 4 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL4 PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.1e-24
Identity = 48/89 (53.93%), Postives = 69/89 (77.53%), Query Frame = 0


Query: 109  GNSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYG 168
            G+   + A+S LFE+C AN+W+PP F+ C+EEGPSH++K+ +KV+VE+KGAS  +LEC+ 
Sbjct: 1563 GDMVHKTARSFLFELCAANYWKPPEFKLCKEEGPSHLRKFTYKVVVEIKGASATLLECHS 1622

Query: 169  NPQTRKKVAAEHAAEGALWYLKHLGYRSK 198
            + + +KK A EHAA+GALW LK LG+  K
Sbjct: 1623 DGKLQKKAAQEHAAQGALWCLKQLGHLPK 1651

[Close]

BLAST of Lag0031255.1 vs. ExPASy Swiss-Prot
Match: P84634 (Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2)

HSP 1 Score: 112.1 bits (279), Expect = 7.4e-24
Identity = 49/88 (55.68%), Postives = 61/88 (69.32%), Query Frame = 0


Query: 107  KEGNSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLEC 166
            K G    + AKSLL E C AN W+PP FECCEEEGP H+K + +KVI+E++ A    LEC
Sbjct: 1613 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLEC 1672

Query: 167  YGNPQTRKKVAAEHAAEGALWYLKHLGY 195
            YG  +  KK AAEHAA+ A+W LKH G+
Sbjct: 1673 YGEARATKKGAAEHAAQAAIWCLKHSGF 1700

[Close]

BLAST of Lag0031255.1 vs. ExPASy Swiss-Prot
Match: Q9M8N2 (Ribonuclease 3-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RTL1 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 7.6e-21
Identity = 60/187 (32.09%), Postives = 105/187 (56.15%), Query Frame = 0


Query: 10  RKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPEMKPNEYSDSA 69
           R + + LK++PP+        + SSIP     ++    V        + + K  E  ++ 
Sbjct: 15  RSIIISLKDIPPL--------DPSSIPSMKPMAQDHHNV-GMQRFQEKTDFKFEEEDNAI 74

Query: 70  RALNTKTVPEASSDSFDKKKE---NSREIASTCSCGHGSTKEGNSEKRAAKSLLFEICTA 129
            + +   +   S+ S   +K+      E  +T +       E  + + +AKS+L E+C +
Sbjct: 75  SSFSNIQIDPNSTRSISLEKKLAPKPDEEHTTTTKPISKDDESKTRRGSAKSVLHEMCAS 134

Query: 130 NHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGAS-EAVLECYGNPQTRKKVAAEHAAEGA 189
             W+PP++ECC  +GP H++ + +KV+VE++ +S + VLEC+G+P+ +KK AAEHAAEGA
Sbjct: 135 KRWRPPVYECCNVDGPCHLRLFTYKVMVEIRDSSGKTVLECFGDPRRKKKAAAEHAAEGA 192

Query: 190 LWYLKHL 193
           LWYL+H+
Sbjct: 195 LWYLEHV 192

[Close]

BLAST of Lag0031255.1 vs. ExPASy TrEMBL
Match: A0A6J1DJ12 (uncharacterized protein LOC111020939 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020939 PE=4 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 1.1e-78
Identity = 155/195 (79.49%), Postives = 166/195 (85.13%), Query Frame = 0


Query: 1   MENTPSSYPRKLNVDLKNLPPINNLRIGNGNRS-SIPVKFEKSRYIRRVPQFMPPNHRPE 60
           ME TP  YPR+LNV+LKNLPPIN  R GNGN   SIP KFEKSRYIRRVP F PPNHRPE
Sbjct: 1   MEQTP-PYPRRLNVNLKNLPPINP-RTGNGNHHFSIPAKFEKSRYIRRVPHFTPPNHRPE 60

Query: 61  MKPNEYSDSARALNTKTVPEASSDSFDKKKENSREIASTCSCGHGSTKEGNSEKRAAKSL 120
           MKP    D+A ALNTKT+PEASSDSF  K ENSREIASTCSCGH S KEG  EKRAAKSL
Sbjct: 61  MKP----DTAGALNTKTIPEASSDSFVAKNENSREIASTCSCGHRSAKEGTLEKRAAKSL 120

Query: 121 LFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGNPQTRKKVAAE 180
           LFE+CTAN+W+PPLFECCEEEGPSH KK+RFKVIVEMKGA +AVLECYGN Q RKKVAA+
Sbjct: 121 LFEVCTANYWKPPLFECCEEEGPSHAKKFRFKVIVEMKGACDAVLECYGNSQARKKVAAQ 180

Query: 181 HAAEGALWYLKHLGY 195
           +AAEGALW LKHLGY
Sbjct: 181 NAAEGALWCLKHLGY 189

[Close]

BLAST of Lag0031255.1 vs. ExPASy TrEMBL
Match: A0A0A0KYI7 (DRBM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006230 PE=4 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 1.2e-77
Identity = 153/209 (73.21%), Postives = 170/209 (81.34%), Query Frame = 0


Query: 1   MENTPSSYPRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPEM 60
           MEN PS  P +LN++LKNLPPINN R  +G+ SSIP KFEKSRYIRRVPQFMP +HRPE+
Sbjct: 19  MENNPSCPPPRLNINLKNLPPINNPRNVSGSHSSIPAKFEKSRYIRRVPQFMPSDHRPEI 78

Query: 61  KPNE---------YSDSARALNTKTVPEA-SSDSFDKKKENSREIASTCS-CGHGSTKEG 120
           + N+         YSDSARALNTKT P A S+DSF +KK+  +++AS CS C H STKEG
Sbjct: 79  RSNDYAGLPMKLAYSDSARALNTKTFPVAPSADSFVRKKDTCKDLASRCSCCRHDSTKEG 138

Query: 121 NSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGN 180
             EKRAAKSLLFEICTANHWQPPLFECCEEEGPSH KKYRFKV +EMKG  EAV+ECYGN
Sbjct: 139 TPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHAKKYRFKVRIEMKGDCEAVVECYGN 198

Query: 181 PQTRKKVAAEHAAEGALWYLKHLGYRSKR 199
            QTRKKVAAEHAAEGALWYL HLGYR KR
Sbjct: 199 LQTRKKVAAEHAAEGALWYLNHLGYRFKR 227

[Close]

BLAST of Lag0031255.1 vs. ExPASy TrEMBL
Match: A0A5D3E0Z9 (Ribonuclease 3-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001930 PE=4 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 2.8e-74
Identity = 147/210 (70.00%), Postives = 168/210 (80.00%), Query Frame = 0


Query: 1   MENTPSSYPRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPEM 60
           ME    S P +LN++LKNLPPINN R   G  SSIP KFEKSRYIRRVPQF+P +HRPE+
Sbjct: 19  MEKNNPSCPPRLNINLKNLPPINNPRNVTGTHSSIPAKFEKSRYIRRVPQFIPFDHRPEI 78

Query: 61  KPNEY----------SDSARALNTKTVPEA-SSDSFDKKKENSREIASTCS-CGHGSTKE 120
           + N Y          SDSARA+NTKT+P A S+D F +KK+N +++AS CS C HGSTKE
Sbjct: 79  RSNNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKDNCKDLASRCSCCRHGSTKE 138

Query: 121 GNSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYG 180
           G  EKRAAKSLLFEICTANHWQPPLFECCEEEGPSH +KYRFKV +EMKG  EAV+ECYG
Sbjct: 139 GTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVSIEMKGDCEAVVECYG 198

Query: 181 NPQTRKKVAAEHAAEGALWYLKHLGYRSKR 199
           N QTRKK+AAEHAAEGALWYLK+LGYR +R
Sbjct: 199 NLQTRKKMAAEHAAEGALWYLKNLGYRFER 228

[Close]

BLAST of Lag0031255.1 vs. ExPASy TrEMBL
Match: A0A1S3BXK0 (uncharacterized protein LOC103494682 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494682 PE=4 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 2.8e-74
Identity = 147/210 (70.00%), Postives = 168/210 (80.00%), Query Frame = 0


Query: 1   MENTPSSYPRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPEM 60
           ME    S P +LN++LKNLPPINN R   G  SSIP KFEKSRYIRRVPQF+P +HRPE+
Sbjct: 19  MEKNNPSCPPRLNINLKNLPPINNPRNVTGTHSSIPAKFEKSRYIRRVPQFIPFDHRPEI 78

Query: 61  KPNEY----------SDSARALNTKTVPEA-SSDSFDKKKENSREIASTCS-CGHGSTKE 120
           + N Y          SDSARA+NTKT+P A S+D F +KK+N +++AS CS C HGSTKE
Sbjct: 79  RSNNYPGLPAMKLACSDSARAMNTKTIPVAPSADYFVQKKDNCKDLASRCSCCRHGSTKE 138

Query: 121 GNSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYG 180
           G  EKRAAKSLLFEICTANHWQPPLFECCEEEGPSH +KYRFKV +EMKG  EAV+ECYG
Sbjct: 139 GTPEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHARKYRFKVSIEMKGDCEAVVECYG 198

Query: 181 NPQTRKKVAAEHAAEGALWYLKHLGYRSKR 199
           N QTRKK+AAEHAAEGALWYLK+LGYR +R
Sbjct: 199 NLQTRKKMAAEHAAEGALWYLKNLGYRFER 228

[Close]

BLAST of Lag0031255.1 vs. ExPASy TrEMBL
Match: A0A6J1GWD5 (ribonuclease 3-like protein 1 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111458030 PE=4 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.5e-67
Identity = 137/204 (67.16%), Postives = 155/204 (75.98%), Query Frame = 0


Query: 1   MENTPSSYPRKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPEM 60
           MENTPS  PR +NV+LKNLPPI+N  +GN N SSIP KFEKSRYI RVPQFMPPNH P++
Sbjct: 1   MENTPSC-PRSVNVNLKNLPPIHNPCVGNANHSSIPAKFEKSRYIGRVPQFMPPNHSPDV 60

Query: 61  KPNEYS---------DSARALNTKTVPEASSDSFDKKKENSREIASTCS-CGHGSTKEGN 120
           +PNEY          DSAR+ N +T P+       ++KEN  + AS  S C H STKEG 
Sbjct: 61  RPNEYDGVPMNPACFDSARSPNARTTPK-------ERKENFGDSASRSSGCSHDSTKEGT 120

Query: 121 SEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLECYGNP 180
           S K++AKSLL+EICTANHW+PPLFECCEEEGPSH KKY FKVIVEMKG     LECYG P
Sbjct: 121 SVKKSAKSLLYEICTANHWKPPLFECCEEEGPSHAKKYTFKVIVEMKGDCGEDLECYGKP 180

Query: 181 QTRKKVAAEHAAEGALWYLKHLGY 195
           QTRKKVAAE AAEGA+WYLKHLGY
Sbjct: 181 QTRKKVAAEDAAEGAVWYLKHLGY 196

[Close]

BLAST of Lag0031255.1 vs. TAIR 10
Match: AT5G20320.1 (dicer-like 4 )

HSP 1 Score: 112.1 bits (279), Expect = 5.2e-25
Identity = 49/88 (55.68%), Postives = 61/88 (69.32%), Query Frame = 0


Query: 107  KEGNSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLEC 166
            K G    + AKSLL E C AN W+PP FECCEEEGP H+K + +KVI+E++ A    LEC
Sbjct: 1613 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLEC 1672

Query: 167  YGNPQTRKKVAAEHAAEGALWYLKHLGY 195
            YG  +  KK AAEHAA+ A+W LKH G+
Sbjct: 1673 YGEARATKKGAAEHAAQAAIWCLKHSGF 1700

[Close]

BLAST of Lag0031255.1 vs. TAIR 10
Match: AT5G20320.2 (dicer-like 4 )

HSP 1 Score: 112.1 bits (279), Expect = 5.2e-25
Identity = 49/88 (55.68%), Postives = 61/88 (69.32%), Query Frame = 0


Query: 107  KEGNSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASEAVLEC 166
            K G    + AKSLL E C AN W+PP FECCEEEGP H+K + +KVI+E++ A    LEC
Sbjct: 1599 KAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMTLEC 1658

Query: 167  YGNPQTRKKVAAEHAAEGALWYLKHLGY 195
            YG  +  KK AAEHAA+ A+W LKH G+
Sbjct: 1659 YGEARATKKGAAEHAAQAAIWCLKHSGF 1686

[Close]

BLAST of Lag0031255.1 vs. TAIR 10
Match: AT1G80650.1 (RNAse THREE-like protein 1 )

HSP 1 Score: 102.1 bits (253), Expect = 5.4e-22
Identity = 60/187 (32.09%), Postives = 105/187 (56.15%), Query Frame = 0


Query: 10  RKLNVDLKNLPPINNLRIGNGNRSSIPVKFEKSRYIRRVPQFMPPNHRPEMKPNEYSDSA 69
           R + + LK++PP+        + SSIP     ++    V        + + K  E  ++ 
Sbjct: 15  RSIIISLKDIPPL--------DPSSIPSMKPMAQDHHNV-GMQRFQEKTDFKFEEEDNAI 74

Query: 70  RALNTKTVPEASSDSFDKKKE---NSREIASTCSCGHGSTKEGNSEKRAAKSLLFEICTA 129
            + +   +   S+ S   +K+      E  +T +       E  + + +AKS+L E+C +
Sbjct: 75  SSFSNIQIDPNSTRSISLEKKLAPKPDEEHTTTTKPISKDDESKTRRGSAKSVLHEMCAS 134

Query: 130 NHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGAS-EAVLECYGNPQTRKKVAAEHAAEGA 189
             W+PP++ECC  +GP H++ + +KV+VE++ +S + VLEC+G+P+ +KK AAEHAAEGA
Sbjct: 135 KRWRPPVYECCNVDGPCHLRLFTYKVMVEIRDSSGKTVLECFGDPRRKKKAAAEHAAEGA 192

Query: 190 LWYLKHL 193
           LWYL+H+
Sbjct: 195 LWYLEHV 192

[Close]

BLAST of Lag0031255.1 vs. TAIR 10
Match: AT4G00420.2 (Double-stranded RNA-binding domain (DsRBD)-containing protein )

HSP 1 Score: 99.4 bits (246), Expect = 3.5e-21
Identity = 48/90 (53.33%), Postives = 64/90 (71.11%), Query Frame = 0


Query: 106 TKEGNSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASE-AVL 165
           T E  +++ +AKS L+ +C+  HW+ PL+E    EGP H+K +  KV VEMK  S   VL
Sbjct: 75  TTEEETQRSSAKSQLYNLCSVRHWKAPLYEYI-AEGPCHMKIFTGKVTVEMKEDSRITVL 134

Query: 166 ECYGNPQTRKKVAAEHAAEGALWYLKHLGY 195
           EC+GNPQ +KK+AAE AAE ALWYLK++GY
Sbjct: 135 ECFGNPQYKKKIAAEQAAEAALWYLKNVGY 163

[Close]

BLAST of Lag0031255.1 vs. TAIR 10
Match: AT4G00420.3 (Double-stranded RNA-binding domain (DsRBD)-containing protein )

HSP 1 Score: 99.4 bits (246), Expect = 3.5e-21
Identity = 48/90 (53.33%), Postives = 64/90 (71.11%), Query Frame = 0


Query: 106 TKEGNSEKRAAKSLLFEICTANHWQPPLFECCEEEGPSHVKKYRFKVIVEMKGASE-AVL 165
           T E  +++ +AKS L+ +C+  HW+ PL+E    EGP H+K +  KV VEMK  S   VL
Sbjct: 53  TTEEETQRSSAKSQLYNLCSVRHWKAPLYEYI-AEGPCHMKIFTGKVTVEMKEDSRITVL 112

Query: 166 ECYGNPQTRKKVAAEHAAEGALWYLKHLGY 195
           EC+GNPQ +KK+AAE AAE ALWYLK++GY
Sbjct: 113 ECFGNPQYKKKIAAEQAAEAALWYLKNVGY 141

The following BLAST results are available for this feature:

BLAST of Lag0031255.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878793.1	1.3e-78	78.05	ribonuclease 3-like protein 1 isoform X3 [Benincasa hispida]	[more]
XP_038878769.1	1.3e-78	78.05	ribonuclease 3-like protein 1 isoform X1 [Benincasa hispida] >XP_038878776.1 rib...	[more]
XP_022153429.1	2.2e-78	79.49	uncharacterized protein LOC111020939 isoform X1 [Momordica charantia]	[more]
XP_004152191.1	2.5e-77	73.21	uncharacterized protein LOC101212514 [Cucumis sativus] >KGN52916.1 hypothetical ...	[more]
XP_038878786.1	4.4e-74	78.35	ribonuclease 3-like protein 1 isoform X2 [Benincasa hispida]	[more]

BLAST of Lag0031255.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7LFZ6	1.1e-24	53.93	Endoribonuclease Dicer homolog 4 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL...	[more]
P84634	7.4e-24	55.68	Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2	[more]
Q9M8N2	7.6e-21	32.09	Ribonuclease 3-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RTL1 PE=2 SV=1	[more]

BLAST of Lag0031255.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DJ12	1.1e-78	79.49	uncharacterized protein LOC111020939 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A0A0KYI7	1.2e-77	73.21	DRBM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006230 PE=4 S...	[more]
A0A5D3E0Z9	2.8e-74	70.00	Ribonuclease 3-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3BXK0	2.8e-74	70.00	uncharacterized protein LOC103494682 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1GWD5	1.5e-67	67.16	ribonuclease 3-like protein 1 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of Lag0031255.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20320.1	5.2e-25	55.68	dicer-like 4	[more]
AT5G20320.2	5.2e-25	55.68	dicer-like 4	[more]
AT1G80650.1	5.4e-22	32.09	RNAse THREE-like protein 1	[more]
AT4G00420.2	3.5e-21	53.33	Double-stranded RNA-binding domain (DsRBD)-containing protein	[more]
AT4G00420.3	3.5e-21	53.33	Double-stranded RNA-binding domain (DsRBD)-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014720	Double-stranded RNA-binding domain	SMART	SM00358	DRBM_3	coord: 116..190 e-value: 2.8E-8 score: 43.5
IPR014720	Double-stranded RNA-binding domain	PROSITE	PS50137	DS_RBD	coord: 115..191 score: 9.173605
None	No IPR available	GENE3D	3.30.160.20		coord: 122..191 e-value: 4.4E-22 score: 80.4
None	No IPR available	PFAM	PF14709	DND1_DSRM	coord: 116..190 e-value: 2.2E-14 score: 53.5
None	No IPR available	PANTHER	PTHR14950:SF67	DOUBLE-STRANDED RNA-BINDING DOMAIN (DSRBD)-CONTAINING PROTEIN	coord: 15..198
None	No IPR available	PANTHER	PTHR14950	DICER-RELATED	coord: 15..198
None	No IPR available	SUPERFAMILY	54768	dsRNA-binding domain-like	coord: 116..194

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0031255	Lag0031255	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0031255.1.exon4	Lag0031255.1.exon4	exon
Lag0031255.1.exon3	Lag0031255.1.exon3	exon
Lag0031255.1.exon2	Lag0031255.1.exon2	exon
Lag0031255.1.exon1	Lag0031255.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0031255.1	cds.Lag0031255.1_4	CDS
cds.Lag0031255.1	cds.Lag0031255.1_3	CDS
cds.Lag0031255.1	cds.Lag0031255.1_2	CDS
cds.Lag0031255.1	cds.Lag0031255.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0031255.1	Lag0031255.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030422	production of siRNA involved in RNA interference
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0004525	ribonuclease III activity
molecular_function	GO:0003723	RNA binding