Lag0029407.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0029407.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	RING-type E3 ubiquitin transferase
Location	chr8: 38672890 .. 38673858 (+)
Sequence length	969
RNA-Seq Expression	Lag0029407.1
Synteny	Lag0029407.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCTCCGCCTCCAATCTCGCCGCCGATAAGCCTTTCTTCTGCCACCAGTGTGATCGCGCCGTCACTGTTACTGTATCCACCTCCTCCGATCCTCTCTGCCCATTTTGCAACGGGGGGTTTCTTGAAGAGTACGAGAATTTCAGTTCCAACTCCAATGCCAATCTTGATCCTAGGTCATTTTTTCTTCCCGATCCTTTTCGTAGTTTTAATCCCTTTGTTTTCTCCTCTTCGAGGGTCGTTGATCTTCAAAACCCTGGTTTATTTTCAAGAACTTTGAGTCCGGCTCCCCAGCCTTGGAATAGGCATCAATCCGATTCGTCAAATCAGTCGGAGCCGTTCGACCCATTTGTTTTTCTTCAGAATCATTTACGGGGCATCTTCGATAGTGGTGCCGATGTTGTGTTTGAAATTCAGGGTAATCCTTCGCACTCTGGCGTTTCAATGCCTCAGAATGTTGGCGATTACTTCATCGGACCTGGCCTCGAGCAGCTCATTCAATTGCTGGCCGAGAATGATCCGAACCGATATGGAACTCCACCGGCTTCGAAATCGGCGATTGAGAGGCTTCCGACCGTGTCTGTTTGTGACGATCTTCTGAATTCGGAAATGAACCAGTGTGCTGTCTGCATAGATGGGTTTGAAAAAGGTATTCAGGTTAAGCAGATGCCTTGCAAGCATGTATTTCACGATTATTGTATTTTACCTTGGCTTGAATTGCACAATTCATGCCCTGTTTGCCGATTCGAGTTGCCTACGGATGATTCTGATTACGAGAATCGAACTCGTGGTCGCCGGGTGCAGGCGAATGAATCTTCCGGAGAAGAATCAGGGTCAGGACACGGTGGGAGCAGTAGGGAGCGTGTTGAGAGGAGGTTTAGAGTGCCGCTATGGTGGCCATTTGGAGGCAGGAACGATGGGAGCGACTCTGGTGGTGGAAATGACAGCGACGGAGAGGGTAGGCCGTAG

mRNA sequence

ATGTCCTCCGCCTCCAATCTCGCCGCCGATAAGCCTTTCTTCTGCCACCAGTGTGATCGCGCCGTCACTGTTACTGTATCCACCTCCTCCGATCCTCTCTGCCCATTTTGCAACGGGGGGTTTCTTGAAGAGTACGAGAATTTCAGTTCCAACTCCAATGCCAATCTTGATCCTAGGTCATTTTTTCTTCCCGATCCTTTTCGTAGTTTTAATCCCTTTGTTTTCTCCTCTTCGAGGGTCGTTGATCTTCAAAACCCTGGTTTATTTTCAAGAACTTTGAGTCCGGCTCCCCAGCCTTGGAATAGGCATCAATCCGATTCGTCAAATCAGTCGGAGCCGTTCGACCCATTTGTTTTTCTTCAGAATCATTTACGGGGCATCTTCGATAGTGGTGCCGATGTTGTGTTTGAAATTCAGGGTAATCCTTCGCACTCTGGCGTTTCAATGCCTCAGAATGTTGGCGATTACTTCATCGGACCTGGCCTCGAGCAGCTCATTCAATTGCTGGCCGAGAATGATCCGAACCGATATGGAACTCCACCGGCTTCGAAATCGGCGATTGAGAGGCTTCCGACCGTGTCTGTTTGTGACGATCTTCTGAATTCGGAAATGAACCAGTGTGCTGTCTGCATAGATGGGTTTGAAAAAGGTATTCAGGTTAAGCAGATGCCTTGCAAGCATGTATTTCACGATTATTGTATTTTACCTTGGCTTGAATTGCACAATTCATGCCCTGTTTGCCGATTCGAGTTGCCTACGGATGATTCTGATTACGAGAATCGAACTCGTGGTCGCCGGGTGCAGGCGAATGAATCTTCCGGAGAAGAATCAGGGTCAGGACACGGTGGGAGCAGTAGGGAGCGTGTTGAGAGGAGGTTTAGAGTGCCGCTATGGTGGCCATTTGGAGGCAGGAACGATGGGAGCGACTCTGGTGGTGGAAATGACAGCGACGGAGAGGGTAGGCCGTAG

Coding sequence (CDS)

ATGTCCTCCGCCTCCAATCTCGCCGCCGATAAGCCTTTCTTCTGCCACCAGTGTGATCGCGCCGTCACTGTTACTGTATCCACCTCCTCCGATCCTCTCTGCCCATTTTGCAACGGGGGGTTTCTTGAAGAGTACGAGAATTTCAGTTCCAACTCCAATGCCAATCTTGATCCTAGGTCATTTTTTCTTCCCGATCCTTTTCGTAGTTTTAATCCCTTTGTTTTCTCCTCTTCGAGGGTCGTTGATCTTCAAAACCCTGGTTTATTTTCAAGAACTTTGAGTCCGGCTCCCCAGCCTTGGAATAGGCATCAATCCGATTCGTCAAATCAGTCGGAGCCGTTCGACCCATTTGTTTTTCTTCAGAATCATTTACGGGGCATCTTCGATAGTGGTGCCGATGTTGTGTTTGAAATTCAGGGTAATCCTTCGCACTCTGGCGTTTCAATGCCTCAGAATGTTGGCGATTACTTCATCGGACCTGGCCTCGAGCAGCTCATTCAATTGCTGGCCGAGAATGATCCGAACCGATATGGAACTCCACCGGCTTCGAAATCGGCGATTGAGAGGCTTCCGACCGTGTCTGTTTGTGACGATCTTCTGAATTCGGAAATGAACCAGTGTGCTGTCTGCATAGATGGGTTTGAAAAAGGTATTCAGGTTAAGCAGATGCCTTGCAAGCATGTATTTCACGATTATTGTATTTTACCTTGGCTTGAATTGCACAATTCATGCCCTGTTTGCCGATTCGAGTTGCCTACGGATGATTCTGATTACGAGAATCGAACTCGTGGTCGCCGGGTGCAGGCGAATGAATCTTCCGGAGAAGAATCAGGGTCAGGACACGGTGGGAGCAGTAGGGAGCGTGTTGAGAGGAGGTTTAGAGTGCCGCTATGGTGGCCATTTGGAGGCAGGAACGATGGGAGCGACTCTGGTGGTGGAAATGACAGCGACGGAGAGGGTAGGCCGTAG

Protein sequence

MSSASNLAADKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEEYENFSSNSNANLDPRSFFLPDPFRSFNPFVFSSSRVVDLQNPGLFSRTLSPAPQPWNRHQSDSSNQSEPFDPFVFLQNHLRGIFDSGADVVFEIQGNPSHSGVSMPQNVGDYFIGPGLEQLIQLLAENDPNRYGTPPASKSAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHVFHDYCILPWLELHNSCPVCRFELPTDDSDYENRTRGRRVQANESSGEESGSGHGGSSRERVERRFRVPLWWPFGGRNDGSDSGGGNDSDGEGRP

Homology

[Close]

BLAST of Lag0029407.1 vs. NCBI nr
Match: XP_023554317.1 (E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023554326.1 E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023554330.1 E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023554338.1 E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023554347.1 E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023554356.1 E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023554365.1 E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023554375.1 E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023554385.1 E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023554392.1 E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023554402.1 E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 565.8 bits (1457), Expect = 2.3e-157
Identity = 271/318 (85.22%), Postives = 287/318 (90.25%), Query Frame = 0


Query: 5   SNLAADKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEEYENFSSNSNANLDPRSFFLP 64
           S+ A DK FFCHQCDR VTV VS S +PLCPFCN GFLEEY+ FS  SNANLD RSFFLP
Sbjct: 2   SSAAVDKSFFCHQCDRDVTVAVSNSLEPLCPFCNEGFLEEYDTFS--SNANLDSRSFFLP 61

Query: 65  DPFRSFNPFVFSSSRVVDLQNPGLFSRTLSPAPQPWNRHQSDSSNQSEPFDPFVFLQNHL 124
           DPFR FNP  FSSS VVDLQNPGLFSRTLSPA Q WNR+QSDSS Q EPFDPFVFLQNHL
Sbjct: 62  DPFRDFNPLAFSSSTVVDLQNPGLFSRTLSPASQLWNRNQSDSSGQPEPFDPFVFLQNHL 121

Query: 125 RGIFDSGADVVFEIQGNPSHSGVSMPQNVGDYFIGPGLEQLIQLLAENDPNRYGTPPASK 184
           RGIFDSGADVVFEIQG+PS SGVSMPQNVGDYFIGPGLEQLIQLLAENDPNR+GTPPASK
Sbjct: 122 RGIFDSGADVVFEIQGHPSRSGVSMPQNVGDYFIGPGLEQLIQLLAENDPNRHGTPPASK 181

Query: 185 SAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHVFHDYCILPWLELHNSC 244
           SAIE+L  V+V +DLLNSEMNQCAVC+DGFEKGI+VKQMPCKH+FHDYC+LPWLELHNSC
Sbjct: 182 SAIEKLQNVTVTEDLLNSEMNQCAVCLDGFEKGIEVKQMPCKHIFHDYCLLPWLELHNSC 241

Query: 245 PVCRFELPTDDSDYENRTRGRRVQANESSGEESGSGHGGSSRERVERRFRVPLWWPFGGR 304
           PVCRFEL TDDSDYENRTRGRRVQANES+GEE GSG+ GSS ERVER FRVPLWWPFGGR
Sbjct: 242 PVCRFELATDDSDYENRTRGRRVQANESNGEEPGSGNSGSSSERVERSFRVPLWWPFGGR 301

Query: 305 NDGSDSGGGNDSDGEGRP 323
           NDGS SGGGNDS+GEG P
Sbjct: 302 NDGSGSGGGNDSEGEGSP 317

[Close]

BLAST of Lag0029407.1 vs. NCBI nr
Match: XP_022960730.1 (E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 565.8 bits (1457), Expect = 2.3e-157
Identity = 270/318 (84.91%), Postives = 287/318 (90.25%), Query Frame = 0


Query: 5   SNLAADKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEEYENFSSNSNANLDPRSFFLP 64
           S+ A DK FFCHQCDR VTV VS S +PLCPFCN GFLEEY+ FS  SNANLD RSFFLP
Sbjct: 2   SSAAVDKSFFCHQCDRDVTVAVSNSLEPLCPFCNEGFLEEYDTFS--SNANLDSRSFFLP 61

Query: 65  DPFRSFNPFVFSSSRVVDLQNPGLFSRTLSPAPQPWNRHQSDSSNQSEPFDPFVFLQNHL 124
           DPFR FNP  FSSS VVDLQNPGLFSRTLSPA Q WNRHQSDSS Q EPFDPFVFLQNHL
Sbjct: 62  DPFRDFNPLAFSSSTVVDLQNPGLFSRTLSPASQLWNRHQSDSSGQPEPFDPFVFLQNHL 121

Query: 125 RGIFDSGADVVFEIQGNPSHSGVSMPQNVGDYFIGPGLEQLIQLLAENDPNRYGTPPASK 184
           RGIFDSGADVVFEIQG+PS SGVSMPQN+GDYFIGPGLEQLIQLLAENDPNR+GTPPASK
Sbjct: 122 RGIFDSGADVVFEIQGHPSRSGVSMPQNLGDYFIGPGLEQLIQLLAENDPNRHGTPPASK 181

Query: 185 SAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHVFHDYCILPWLELHNSC 244
           SAIE+L  V+V ++LLNSEMNQCAVC+DGFEKGI+VKQMPCKH+FHDYC+LPWLELHNSC
Sbjct: 182 SAIEKLQNVTVTEELLNSEMNQCAVCLDGFEKGIEVKQMPCKHIFHDYCLLPWLELHNSC 241

Query: 245 PVCRFELPTDDSDYENRTRGRRVQANESSGEESGSGHGGSSRERVERRFRVPLWWPFGGR 304
           PVCRFEL TDDSDYENRTRGRRVQANES+GEE GSG+ GSS ERVER FRVPLWWPFGGR
Sbjct: 242 PVCRFELATDDSDYENRTRGRRVQANESNGEEPGSGNSGSSSERVERSFRVPLWWPFGGR 301

Query: 305 NDGSDSGGGNDSDGEGRP 323
           NDGS SGGGNDS+GEG P
Sbjct: 302 NDGSGSGGGNDSEGEGSP 317

[Close]

BLAST of Lag0029407.1 vs. NCBI nr
Match: KAG7032683.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 564.7 bits (1454), Expect = 5.1e-157
Identity = 270/318 (84.91%), Postives = 287/318 (90.25%), Query Frame = 0


Query: 5   SNLAADKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEEYENFSSNSNANLDPRSFFLP 64
           S+ A DK FFCHQCDR VTV VS S +PLCPFCN GFLEEY+ FS  SNANLD RSFFLP
Sbjct: 2   SSAAVDKSFFCHQCDRDVTVAVSNSLEPLCPFCNEGFLEEYDTFS--SNANLDSRSFFLP 61

Query: 65  DPFRSFNPFVFSSSRVVDLQNPGLFSRTLSPAPQPWNRHQSDSSNQSEPFDPFVFLQNHL 124
           DPFR FNP  FSSS VVDLQNPGLFSRTLSPA Q WNR+QSDSS Q EPFDPFVFLQNHL
Sbjct: 62  DPFRDFNPLAFSSSTVVDLQNPGLFSRTLSPASQLWNRNQSDSSGQPEPFDPFVFLQNHL 121

Query: 125 RGIFDSGADVVFEIQGNPSHSGVSMPQNVGDYFIGPGLEQLIQLLAENDPNRYGTPPASK 184
           RGIFDSGADVVFEIQG+PS SGVSMPQN+GDYFIGPGLEQLIQLLAENDPNR+GTPPASK
Sbjct: 122 RGIFDSGADVVFEIQGHPSRSGVSMPQNLGDYFIGPGLEQLIQLLAENDPNRHGTPPASK 181

Query: 185 SAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHVFHDYCILPWLELHNSC 244
           SAIE+L  V+V D+LLNSEMNQCAVC+DGFEKGI+VKQMPCKH+FHDYC+LPWLELHNSC
Sbjct: 182 SAIEKLQNVTVTDELLNSEMNQCAVCLDGFEKGIEVKQMPCKHIFHDYCLLPWLELHNSC 241

Query: 245 PVCRFELPTDDSDYENRTRGRRVQANESSGEESGSGHGGSSRERVERRFRVPLWWPFGGR 304
           PVCRFEL TDDSDYENRTRGRRVQANES+GEE GSG+ GSS ERVER FRVPLWWPFGGR
Sbjct: 242 PVCRFELATDDSDYENRTRGRRVQANESNGEEPGSGNSGSSSERVERSFRVPLWWPFGGR 301

Query: 305 NDGSDSGGGNDSDGEGRP 323
           NDGS SGGGNDS+GEG P
Sbjct: 302 NDGSGSGGGNDSEGEGSP 317

[Close]

BLAST of Lag0029407.1 vs. NCBI nr
Match: KAG6601985.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 564.7 bits (1454), Expect = 5.1e-157
Identity = 270/318 (84.91%), Postives = 287/318 (90.25%), Query Frame = 0


Query: 5   SNLAADKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEEYENFSSNSNANLDPRSFFLP 64
           S+ A DK FFCHQCDR VTV VS S +PLCPFCN GFLEEY+ FS  SNANLD RSFFLP
Sbjct: 2   SSAAVDKSFFCHQCDRDVTVAVSNSLEPLCPFCNEGFLEEYDTFS--SNANLDSRSFFLP 61

Query: 65  DPFRSFNPFVFSSSRVVDLQNPGLFSRTLSPAPQPWNRHQSDSSNQSEPFDPFVFLQNHL 124
           DPFR FNP  FSSS VVDLQNPGLFSRTLSPA Q WNR+QSDSS Q EPFDPFVFLQNHL
Sbjct: 62  DPFRDFNPLAFSSSTVVDLQNPGLFSRTLSPASQLWNRNQSDSSGQPEPFDPFVFLQNHL 121

Query: 125 RGIFDSGADVVFEIQGNPSHSGVSMPQNVGDYFIGPGLEQLIQLLAENDPNRYGTPPASK 184
           RGIFDSGADVVFEIQG+PS SGVSMPQN+GDYFIGPGLEQLIQLLAENDPNR+GTPPASK
Sbjct: 122 RGIFDSGADVVFEIQGHPSRSGVSMPQNLGDYFIGPGLEQLIQLLAENDPNRHGTPPASK 181

Query: 185 SAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHVFHDYCILPWLELHNSC 244
           SAIE+L  V+V D+LLNSEMNQCAVC+DGFEKGI+VKQMPCKH+FHDYC+LPWLELHNSC
Sbjct: 182 SAIEKLQNVTVTDELLNSEMNQCAVCLDGFEKGIEVKQMPCKHIFHDYCLLPWLELHNSC 241

Query: 245 PVCRFELPTDDSDYENRTRGRRVQANESSGEESGSGHGGSSRERVERRFRVPLWWPFGGR 304
           PVCRFEL TDDSDYENRTRGRRVQANES+GEE GSG+ GSS ERVER FRVPLWWPFGGR
Sbjct: 242 PVCRFELATDDSDYENRTRGRRVQANESNGEEPGSGNSGSSSERVERSFRVPLWWPFGGR 301

Query: 305 NDGSDSGGGNDSDGEGRP 323
           NDGS SGGGNDS+GEG P
Sbjct: 302 NDGSGSGGGNDSEGEGSP 317

[Close]

BLAST of Lag0029407.1 vs. NCBI nr
Match: XP_022990139.1 (E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 561.2 bits (1445), Expect = 5.7e-156
Identity = 270/318 (84.91%), Postives = 286/318 (89.94%), Query Frame = 0


Query: 5   SNLAADKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEEYENFSSNSNANLDPRSFFLP 64
           S+ A DK FFCHQCDR VTV VS S +PLCPFCN GFLEEY+ FS  SNANLD RSFFL 
Sbjct: 2   SSAAVDKSFFCHQCDRDVTVAVSNSLEPLCPFCNEGFLEEYDTFS--SNANLDSRSFFLL 61

Query: 65  DPFRSFNPFVFSSSRVVDLQNPGLFSRTLSPAPQPWNRHQSDSSNQSEPFDPFVFLQNHL 124
           DPFR FNP  FSSS VVDLQNPGLFSRTLSPA Q WNR+QSDSS Q EPFDPFVFLQNHL
Sbjct: 62  DPFRDFNPLAFSSSTVVDLQNPGLFSRTLSPASQLWNRNQSDSSGQPEPFDPFVFLQNHL 121

Query: 125 RGIFDSGADVVFEIQGNPSHSGVSMPQNVGDYFIGPGLEQLIQLLAENDPNRYGTPPASK 184
           RGIFDSGADVVFEIQG+PS SGVSMPQNVGDYFIGPGLEQLIQLLAENDPNR+GTPPASK
Sbjct: 122 RGIFDSGADVVFEIQGHPSLSGVSMPQNVGDYFIGPGLEQLIQLLAENDPNRHGTPPASK 181

Query: 185 SAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHVFHDYCILPWLELHNSC 244
           SAIE+L  V+V +DLLNSEMNQCAVC+DGFEKGI+VKQMPCKH+FHDYC+LPWLELHNSC
Sbjct: 182 SAIEKLQNVTVTEDLLNSEMNQCAVCLDGFEKGIEVKQMPCKHIFHDYCLLPWLELHNSC 241

Query: 245 PVCRFELPTDDSDYENRTRGRRVQANESSGEESGSGHGGSSRERVERRFRVPLWWPFGGR 304
           PVCRFEL TDDSDYENRTRGRRVQANES+GEE GSG+ GSS ERVER FRVPLWWPFGGR
Sbjct: 242 PVCRFELATDDSDYENRTRGRRVQANESNGEEPGSGNSGSSSERVERSFRVPLWWPFGGR 301

Query: 305 NDGSDSGGGNDSDGEGRP 323
           NDGS SGGGNDS+GEG P
Sbjct: 302 NDGSSSGGGNDSEGEGSP 317

[Close]

BLAST of Lag0029407.1 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 3.3e-90
Identity = 177/324 (54.63%), Postives = 214/324 (66.05%), Query Frame = 0


Query: 2   SSASNLAADKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEEYENFSSNSNANLDPRS- 61
           S+A+    DK FFC+QC++ VT+++S+S+DP CP CN GFLEEYE+ + N + N +P S 
Sbjct: 10  SAAAAPEVDKMFFCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSS 69

Query: 62  ---FFLPDPFRSFNPFVFSSSRVVDLQNPGL-FSRTLSPAPQPWNRHQSDSSNQSEPFDP 121
              F + DPF +  P +F SS        G+ F     P+ QP  R  +  + QS+ FDP
Sbjct: 70  DSFFPMADPFSTLLPLIFGSSAAAP---SGMDFMSLFGPSMQPQAR-STQQNPQSDAFDP 129

Query: 122 FVFLQNHLRGIFDSGADVVFEIQGNPSHSGVSMPQNVGDYFIGPGLEQLIQLLAENDPNR 181
           F FLQNHL+ +  SG    F I+ +PS  G  MP N GDYF GPGLEQLIQ LAENDPNR
Sbjct: 130 FTFLQNHLQTLRSSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNR 189

Query: 182 YGTPPASKSAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHVFHDYCILP 241
           YGTPPASKSAI+ LPTV V  D+L SEMNQCAVC+D FE G  VKQMPCKHVFH  C+LP
Sbjct: 190 YGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLP 249

Query: 242 WLELHNSCPVCRFELPTDDSDYENRTRGRRVQANESSGEESGSGHGGSSRERVERRFRVP 301
           WLELHNSCPVCRFELPTDD DYENR++G     ++ SG+  GS  G  +      RF + 
Sbjct: 250 WLELHNSCPVCRFELPTDDPDYENRSQG-----SQGSGDGQGSVEGQQT-----PRFSIQ 309

Query: 302 LWWPFGGRNDGSDSGGGNDSDGEG 321
           L WPF  R DGS SG G    G G
Sbjct: 310 LPWPF-RRQDGSGSGSGAPGTGGG 318

[Close]

BLAST of Lag0029407.1 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 1.5e-87
Identity = 173/329 (52.58%), Postives = 205/329 (62.31%), Query Frame = 0


Query: 10  DKPFFCHQCDRAVTVTVS-TSSDPLCPFCNGGFLEEYENFSSNSNA---NLDPRS----- 69
           +KPFFC+QC+R V VT+S  SSDP CP CN GFLEEY+N + N  +   N +P S     
Sbjct: 20  NKPFFCYQCNRTVNVTISPPSSDPTCPICNEGFLEEYDNPNPNQGSGFLNPNPNSIPFHD 79

Query: 70  --FFLPDPFRSFNPFVF--------SSSRVVDLQNPGLFSRTLSPAPQPWNRHQSDSSNQ 129
               L DPF S  P +F        SSS  +D  NP L   T S    P+          
Sbjct: 80  LFLTLSDPFASLLPLLFPSSSSTTTSSSASIDPNNPSLSGPTRSGRGDPF---------- 139

Query: 130 SEPFDPFVFLQNHLRGIFDSGADVVFEIQGNPSHSGVSMPQNVGDYFIGPGLEQLIQLLA 189
              FDPF F+QNHL  +  SGA + F IQ NPS  G  +P N+GDYFIGPGLEQLIQ LA
Sbjct: 140 --AFDPFTFIQNHLNDLRSSGAQIEFVIQNNPSDQGFRLPANIGDYFIGPGLEQLIQQLA 199

Query: 190 ENDPNRYGTPPASKSAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHVFH 249
           ENDPNRYGTPPASKSAIE LP V++    LNSE NQCAVC+D FE+G + KQMPCKH++H
Sbjct: 200 ENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYH 259

Query: 250 DYCILPWLELHNSCPVCRFELPTDDSDYENRTRGRRVQANESSGEESGSGHGGSSRERVE 309
             C+LPWLELHNSCPVCR ELPTDD DYE R RG +  +  + G+ SG    G +R  VE
Sbjct: 260 KDCLLPWLELHNSCPVCRHELPTDDPDYERRVRGAQGTSGGNDGDNSGQRSDGDNR-TVE 319

Query: 310 RRFRVPLWWPFGGRNDGSDSGGGNDSDGE 320
           R FR+ L WPF  R  G   G   ++  E
Sbjct: 320 RSFRISLPWPFQARGPGPAPGDNAETRQE 335

[Close]

BLAST of Lag0029407.1 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.9e-37
Identity = 115/335 (34.33%), Postives = 161/335 (48.06%), Query Frame = 0


Query: 1   MSSASNLAADKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEE--------YENFSSNS 60
           MSS+ N       +CH+C RAV +      +P+C +C GGF+EE        ++ F S+ 
Sbjct: 1   MSSSRN-----THWCHRCQRAVRL---HGQEPVCFYCGGGFVEELDMAQASPFDMFRSHR 60

Query: 61  NANLDPRSFFLPDPFRSFNPFVFSSSRVVDLQNP-GLFSRTLSPAPQPWNRHQSDSSNQS 120
                 ++F L D F      VF  +R+ +  +   +  RT+S  P+ +           
Sbjct: 61  GVVERDQTFDLMDAFS-----VFMRNRLAERSHDREIRGRTISSGPENF----------- 120

Query: 121 EPFDPFVFL--QNHLRGIFDSGADVVFEIQGNPSHSGVSMPQ-NVGDYFIGPGLEQLIQL 180
               P +    Q   R   D+  + +F    N    G+ + + N GDYF GPGLE+L + 
Sbjct: 121 PGLAPLLIFGGQVPYRLTGDNAVEALF----NGGSPGIGITRGNTGDYFFGPGLEELFEQ 180

Query: 181 LAENDPNRYGTPPASKSAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHV 240
           L+     R G PPA +SAI+ LPT+ +    L S  + C VC D FE G + KQMPC H+
Sbjct: 181 LSAGTTRR-GPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHI 240

Query: 241 FHDYCILPWLELHNSCPVCRFELP--TDDSDYENRTRGRRVQANESSGEESGSGHGGSSR 300
           +H  CI+PWL  HNSCPVCR ELP  +  S  +NRT   R   + SS   S S   G+  
Sbjct: 241 YHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRENGN-- 300

Query: 301 ERVERRFRVPLWWPFGGRNDGSDS----GGGNDSD 318
              ERR     +WPF      S S    GG  +SD
Sbjct: 301 ---ERRNPFSSFWPFRSSGSSSSSTQNRGGTRNSD 301

[Close]

BLAST of Lag0029407.1 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 4.0e-35
Identity = 99/297 (33.33%), Postives = 141/297 (47.47%), Query Frame = 0


Query: 14  FCHQCDRAVTVTVSTSSDPLCPFCNGGFLEEYENFSSNSNANLDPRSFFLPDPFRSFNPF 73
           +CH+C RAV +    + D +C +C GGF+EE +   S ++ +++    F  D   +F+ F
Sbjct: 9   WCHRCQRAVWL---RARDAVCSYCGGGFVEEIDIGPSRAHRDVERDPTF--DLMEAFSAF 68

Query: 74  VFSSSRVVDLQNPGLFSRTLSPAPQPWNRHQSDSSNQSEPFDPFVFL--QNHLRGIFDSG 133
           +   SR+ +      + R +S       R  S  S       P +    Q   R      
Sbjct: 69  M--RSRLAERS----YDREIS------GRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDN 128

Query: 134 ADVVFEIQGNPSHSGVSMPQNVGDYFIGPGLEQLIQLLAENDPNRYGTPPASKSAIERLP 193
           + V   + G     G++   N GDYF GPGLE+LI+ L+    +R G PPA KS+I+ LP
Sbjct: 129 SSVEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHR-GPPPAPKSSIDALP 188

Query: 194 TVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHVFHDYCILPWLELHNSCPVCRFEL 253
           T+ +    L S  + C VC D FE   + KQMPC H++H  CI+PWL  HNSCPVCR EL
Sbjct: 189 TIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKEL 248

Query: 254 PTDDSDYENRTRGRRVQANESSGEESGSGHGGSSRERVERRFRVPLWWPFGGRNDGS 309
           P+  S               SS  +S      + RE   RR      WPF   +  S
Sbjct: 249 PSRGS---------------SSSTQSSQNRSTNGRENSRRRNIFSNLWPFRSSSSSS 272

[Close]

BLAST of Lag0029407.1 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 1.7e-33
Identity = 100/301 (33.22%), Postives = 142/301 (47.18%), Query Frame = 0


Query: 8   AADKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEEYENFSSNSNANLDPRSFFLP--- 67
           AA   ++CH+C++A+   +    +  CP C GGF+EE  +       N  P   F     
Sbjct: 4   AAITRYWCHECEQAIEEAM--VDEIKCPSCGGGFVEEMTDEEIERLTNRQPEPGFSQWNP 63

Query: 68  ---------------DPFRSFNPFVFSSSRVVDLQNPGLFSRTLSPAPQPW-NRHQSDSS 127
                          D  R F  F+    R   L+      R L        +  + DSS
Sbjct: 64  IEHPGETMDSDDEDNDLGREFEGFIRRHRRASTLR------RVLDSIHDDLADDQERDSS 123

Query: 128 NQSEPFDPFVFLQNHLRGIFDSGADVVFEIQGNPSHSGVSMPQNVGDYFIGPGLEQLIQL 187
                F+  + LQ  +    +   D      G+ +  G+     + +Y +G GL  L+Q 
Sbjct: 124 ILINAFNQALALQGSVLDPDEGQGDQ----GGSTNDDGL-----LEEYVLGAGLSLLLQH 183

Query: 188 LAENDPNRYGTPPASKSAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHV 247
           LAE+DP+R GTPPA K A+E LPTV +       E+  C+VC+D  E G Q KQMPC+H 
Sbjct: 184 LAESDPSRNGTPPAKKEAVEALPTVKI------EEVVSCSVCLDDLEVGSQAKQMPCEHK 243

Query: 248 FHDYCILPWLELHNSCPVCRFELPTDDS-DYENRTRGRRVQANESSGEESGSGHGGSSRE 289
           FH  CILPWLELH+SCPVCRFELP++++ D    +   RV+ +       G G+   S  
Sbjct: 244 FHSSCILPWLELHSSCPVCRFELPSEETKDLNEPSNIGRVEDSHEEVRADGPGNVSESSN 281

[Close]

BLAST of Lag0029407.1 vs. ExPASy TrEMBL
Match: A0A6J1H9Y0 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461423 PE=4 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.1e-157
Identity = 270/318 (84.91%), Postives = 287/318 (90.25%), Query Frame = 0


Query: 5   SNLAADKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEEYENFSSNSNANLDPRSFFLP 64
           S+ A DK FFCHQCDR VTV VS S +PLCPFCN GFLEEY+ FS  SNANLD RSFFLP
Sbjct: 2   SSAAVDKSFFCHQCDRDVTVAVSNSLEPLCPFCNEGFLEEYDTFS--SNANLDSRSFFLP 61

Query: 65  DPFRSFNPFVFSSSRVVDLQNPGLFSRTLSPAPQPWNRHQSDSSNQSEPFDPFVFLQNHL 124
           DPFR FNP  FSSS VVDLQNPGLFSRTLSPA Q WNRHQSDSS Q EPFDPFVFLQNHL
Sbjct: 62  DPFRDFNPLAFSSSTVVDLQNPGLFSRTLSPASQLWNRHQSDSSGQPEPFDPFVFLQNHL 121

Query: 125 RGIFDSGADVVFEIQGNPSHSGVSMPQNVGDYFIGPGLEQLIQLLAENDPNRYGTPPASK 184
           RGIFDSGADVVFEIQG+PS SGVSMPQN+GDYFIGPGLEQLIQLLAENDPNR+GTPPASK
Sbjct: 122 RGIFDSGADVVFEIQGHPSRSGVSMPQNLGDYFIGPGLEQLIQLLAENDPNRHGTPPASK 181

Query: 185 SAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHVFHDYCILPWLELHNSC 244
           SAIE+L  V+V ++LLNSEMNQCAVC+DGFEKGI+VKQMPCKH+FHDYC+LPWLELHNSC
Sbjct: 182 SAIEKLQNVTVTEELLNSEMNQCAVCLDGFEKGIEVKQMPCKHIFHDYCLLPWLELHNSC 241

Query: 245 PVCRFELPTDDSDYENRTRGRRVQANESSGEESGSGHGGSSRERVERRFRVPLWWPFGGR 304
           PVCRFEL TDDSDYENRTRGRRVQANES+GEE GSG+ GSS ERVER FRVPLWWPFGGR
Sbjct: 242 PVCRFELATDDSDYENRTRGRRVQANESNGEEPGSGNSGSSSERVERSFRVPLWWPFGGR 301

Query: 305 NDGSDSGGGNDSDGEGRP 323
           NDGS SGGGNDS+GEG P
Sbjct: 302 NDGSGSGGGNDSEGEGSP 317

[Close]

BLAST of Lag0029407.1 vs. ExPASy TrEMBL
Match: A0A6J1JR94 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111487122 PE=4 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 2.8e-156
Identity = 270/318 (84.91%), Postives = 286/318 (89.94%), Query Frame = 0


Query: 5   SNLAADKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEEYENFSSNSNANLDPRSFFLP 64
           S+ A DK FFCHQCDR VTV VS S +PLCPFCN GFLEEY+ FS  SNANLD RSFFL 
Sbjct: 2   SSAAVDKSFFCHQCDRDVTVAVSNSLEPLCPFCNEGFLEEYDTFS--SNANLDSRSFFLL 61

Query: 65  DPFRSFNPFVFSSSRVVDLQNPGLFSRTLSPAPQPWNRHQSDSSNQSEPFDPFVFLQNHL 124
           DPFR FNP  FSSS VVDLQNPGLFSRTLSPA Q WNR+QSDSS Q EPFDPFVFLQNHL
Sbjct: 62  DPFRDFNPLAFSSSTVVDLQNPGLFSRTLSPASQLWNRNQSDSSGQPEPFDPFVFLQNHL 121

Query: 125 RGIFDSGADVVFEIQGNPSHSGVSMPQNVGDYFIGPGLEQLIQLLAENDPNRYGTPPASK 184
           RGIFDSGADVVFEIQG+PS SGVSMPQNVGDYFIGPGLEQLIQLLAENDPNR+GTPPASK
Sbjct: 122 RGIFDSGADVVFEIQGHPSLSGVSMPQNVGDYFIGPGLEQLIQLLAENDPNRHGTPPASK 181

Query: 185 SAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHVFHDYCILPWLELHNSC 244
           SAIE+L  V+V +DLLNSEMNQCAVC+DGFEKGI+VKQMPCKH+FHDYC+LPWLELHNSC
Sbjct: 182 SAIEKLQNVTVTEDLLNSEMNQCAVCLDGFEKGIEVKQMPCKHIFHDYCLLPWLELHNSC 241

Query: 245 PVCRFELPTDDSDYENRTRGRRVQANESSGEESGSGHGGSSRERVERRFRVPLWWPFGGR 304
           PVCRFEL TDDSDYENRTRGRRVQANES+GEE GSG+ GSS ERVER FRVPLWWPFGGR
Sbjct: 242 PVCRFELATDDSDYENRTRGRRVQANESNGEEPGSGNSGSSSERVERSFRVPLWWPFGGR 301

Query: 305 NDGSDSGGGNDSDGEGRP 323
           NDGS SGGGNDS+GEG P
Sbjct: 302 NDGSSSGGGNDSEGEGSP 317

[Close]

BLAST of Lag0029407.1 vs. ExPASy TrEMBL
Match: A0A6J1CK28 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111011877 PE=4 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 5.4e-152
Identity = 266/330 (80.61%), Postives = 290/330 (87.88%), Query Frame = 0


Query: 1   MSSASNLAA-------DKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEEYENFSSNSN 60
           MS A NLAA       DKPFFCHQCDRAVT+TVSTSSDPLCPFCN GFLEEYEN  S+SN
Sbjct: 1   MSFAFNLAAGANSADRDKPFFCHQCDRAVTITVSTSSDPLCPFCNEGFLEEYENSYSDSN 60

Query: 61  ANLDPRSFFLPDPFRSFNPFVF-SSSRVVDLQNPGLFSRTLSPAPQPWNRHQSDSSNQSE 120
            +LD      PDPFR FNP VF SSS V+DLQNPGLFSRT+ PA QPWNR+QSDS+ Q E
Sbjct: 61  DDLDRAPIHFPDPFRGFNPLVFSSSSSVIDLQNPGLFSRTMRPASQPWNRNQSDSTIQPE 120

Query: 121 PFDPFVFLQNHLRGIFDSGADVVFEIQGNPSHSGVSMPQNVGDYFIGPGLEQLIQLLAEN 180
           PFDPF+FLQNHLRGIFDSGADVVFEIQG+PSHS +SMPQNVGDYF+GPGLEQLIQLLAEN
Sbjct: 121 PFDPFLFLQNHLRGIFDSGADVVFEIQGHPSHSVLSMPQNVGDYFVGPGLEQLIQLLAEN 180

Query: 181 DPNRYGTPPASKSAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHVFHDY 240
           DPNRYGTPPASKSAIE+LP V+V +DLLNSEMNQCAVCID F+ GI+VKQMPCKHVFHD 
Sbjct: 181 DPNRYGTPPASKSAIEKLPAVTVTEDLLNSEMNQCAVCIDEFQIGIKVKQMPCKHVFHDD 240

Query: 241 CILPWLELHNSCPVCRFELPTDDSDYENRTRGRRVQANESSGEESGSGHGGSSRERVERR 300
           C+LPWLELHNSCPVCRFELPTDDSDYENRTRGRRVQ NE+SG++SG  + GS  ERVERR
Sbjct: 241 CLLPWLELHNSCPVCRFELPTDDSDYENRTRGRRVQENEASGQQSGLDNSGSG-ERVERR 300

Query: 301 FRVPLWWPFGGRNDGSDSGGGNDSDGEGRP 323
           FRVPLWWPFGGRN+GSDSGGGNDS+ +GRP
Sbjct: 301 FRVPLWWPFGGRNEGSDSGGGNDSEEDGRP 329

[Close]

BLAST of Lag0029407.1 vs. ExPASy TrEMBL
Match: A0A6J1FJU6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111444684 PE=4 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 6.1e-148
Identity = 262/316 (82.91%), Postives = 277/316 (87.66%), Query Frame = 0


Query: 2   SSASNLAADKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEEYENFSSNSNANLDPRSF 61
           S+AS  A DKPFFCHQCDRAVTV VS SSDPLCPFCN GFLEEYEN +SNSN NLD  + 
Sbjct: 3   STASLAAVDKPFFCHQCDRAVTVIVSLSSDPLCPFCNEGFLEEYENSNSNSNVNLDRGTL 62

Query: 62  FLPDPFRSFNPFVFSSSRVVDLQNPGLFSRTLSPAPQPWNRHQSDSSNQSEPFDPFVFLQ 121
            LPDPFR FNP V SSS VVDLQNPGLFSRTLS A       QS+SS Q EP+DPFVFLQ
Sbjct: 63  LLPDPFRGFNPLVVSSSTVVDLQNPGLFSRTLSLA------SQSNSSVQPEPYDPFVFLQ 122

Query: 122 NHLRGIFDSGADVVFEIQGNPSHSGVSMPQNVGDYFIGPGLEQLIQLLAENDPNRYGTPP 181
           NHLRGIFDSGADVVFEI  + SHSG SMPQNVGDYFIGPGLEQLIQLLA+NDPNRYGTPP
Sbjct: 123 NHLRGIFDSGADVVFEIPRHASHSGFSMPQNVGDYFIGPGLEQLIQLLADNDPNRYGTPP 182

Query: 182 ASKSAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHVFHDYCILPWLELH 241
           ASKSAIE+LPTV+V +DLLNSEMNQCAVCIDGFEK  +VKQMPCKHVFHDYCILPWLELH
Sbjct: 183 ASKSAIEKLPTVTVTEDLLNSEMNQCAVCIDGFEKDTKVKQMPCKHVFHDYCILPWLELH 242

Query: 242 NSCPVCRFELPTDDSDYENRTRGRRVQANESSGEESGSGHGGSSRERVERRFRVPLWWPF 301
           NSCPVCRFELPTDDSDYENRTRG +VQANESSGEESGSG+ GS+  RVERRFRVPLWWPF
Sbjct: 243 NSCPVCRFELPTDDSDYENRTRGSQVQANESSGEESGSGNRGSNSGRVERRFRVPLWWPF 302

Query: 302 GGRNDGSDSGGGNDSD 318
           GGRNDGSDSG GN S+
Sbjct: 303 GGRNDGSDSGDGNVSE 312

[Close]

BLAST of Lag0029407.1 vs. ExPASy TrEMBL
Match: A0A6J1K1C4 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111489246 PE=4 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 4.4e-146
Identity = 258/316 (81.65%), Postives = 275/316 (87.03%), Query Frame = 0


Query: 2   SSASNLAADKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEEYENFSSNSNANLDPRSF 61
           S+AS  A DKPFFCHQCDRAVTV +S SSDPLCPFCN GFLEEYEN +SNSN NLD  + 
Sbjct: 3   STASLAAVDKPFFCHQCDRAVTVILSPSSDPLCPFCNEGFLEEYENSNSNSNVNLDRGTL 62

Query: 62  FLPDPFRSFNPFVFSSSRVVDLQNPGLFSRTLSPAPQPWNRHQSDSSNQSEPFDPFVFLQ 121
            LPDPFR FNP V SSS VVDLQNPGLFSRTLS A       QS+SS Q E +DPFVFLQ
Sbjct: 63  LLPDPFRGFNPLVVSSSTVVDLQNPGLFSRTLSLA------SQSNSSVQPEQYDPFVFLQ 122

Query: 122 NHLRGIFDSGADVVFEIQGNPSHSGVSMPQNVGDYFIGPGLEQLIQLLAENDPNRYGTPP 181
           NHLRGIFDSGADVVFEI  + SHSG SMPQNVGDYFIGPGLEQLIQLLA+NDPNRYGTPP
Sbjct: 123 NHLRGIFDSGADVVFEIPRHASHSGFSMPQNVGDYFIGPGLEQLIQLLADNDPNRYGTPP 182

Query: 182 ASKSAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHVFHDYCILPWLELH 241
           ASKS IE+LP V+V +DLLNSEMNQCAVCIDGFEK  +VKQMPCKHVFHDYCILPWLELH
Sbjct: 183 ASKSVIEKLPNVTVTEDLLNSEMNQCAVCIDGFEKDTEVKQMPCKHVFHDYCILPWLELH 242

Query: 242 NSCPVCRFELPTDDSDYENRTRGRRVQANESSGEESGSGHGGSSRERVERRFRVPLWWPF 301
           NSCPVCRFELPTDDSDYENRTRG +VQANESSGEESGSG+ GS+R RVERRFRVPLWWPF
Sbjct: 243 NSCPVCRFELPTDDSDYENRTRGSQVQANESSGEESGSGNRGSNRGRVERRFRVPLWWPF 302

Query: 302 GGRNDGSDSGGGNDSD 318
           GGRNDG+DSG GN S+
Sbjct: 303 GGRNDGNDSGDGNVSE 312

[Close]

BLAST of Lag0029407.1 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 333.2 bits (853), Expect = 2.3e-91
Identity = 177/324 (54.63%), Postives = 214/324 (66.05%), Query Frame = 0


Query: 2   SSASNLAADKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEEYENFSSNSNANLDPRS- 61
           S+A+    DK FFC+QC++ VT+++S+S+DP CP CN GFLEEYE+ + N + N +P S 
Sbjct: 10  SAAAAPEVDKMFFCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSS 69

Query: 62  ---FFLPDPFRSFNPFVFSSSRVVDLQNPGL-FSRTLSPAPQPWNRHQSDSSNQSEPFDP 121
              F + DPF +  P +F SS        G+ F     P+ QP  R  +  + QS+ FDP
Sbjct: 70  DSFFPMADPFSTLLPLIFGSSAAAP---SGMDFMSLFGPSMQPQAR-STQQNPQSDAFDP 129

Query: 122 FVFLQNHLRGIFDSGADVVFEIQGNPSHSGVSMPQNVGDYFIGPGLEQLIQLLAENDPNR 181
           F FLQNHL+ +  SG    F I+ +PS  G  MP N GDYF GPGLEQLIQ LAENDPNR
Sbjct: 130 FTFLQNHLQTLRSSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNR 189

Query: 182 YGTPPASKSAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHVFHDYCILP 241
           YGTPPASKSAI+ LPTV V  D+L SEMNQCAVC+D FE G  VKQMPCKHVFH  C+LP
Sbjct: 190 YGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLP 249

Query: 242 WLELHNSCPVCRFELPTDDSDYENRTRGRRVQANESSGEESGSGHGGSSRERVERRFRVP 301
           WLELHNSCPVCRFELPTDD DYENR++G     ++ SG+  GS  G  +      RF + 
Sbjct: 250 WLELHNSCPVCRFELPTDDPDYENRSQG-----SQGSGDGQGSVEGQQT-----PRFSIQ 309

Query: 302 LWWPFGGRNDGSDSGGGNDSDGEG 321
           L WPF  R DGS SG G    G G
Sbjct: 310 LPWPF-RRQDGSGSGSGAPGTGGG 318

[Close]

BLAST of Lag0029407.1 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 162.2 bits (409), Expect = 7.2e-40
Identity = 92/192 (47.92%), Postives = 113/192 (58.85%), Query Frame = 0


Query: 142 PSHSGVSMPQ--NVGDYFIGPGLEQLIQLLAENDPNRYGTPPASKSAIERLPTVSVCDDL 201
           PS    + P   ++GDYFIGPGL+ L+Q LAENDPNR GTPPA K A+E LPTV + + L
Sbjct: 198 PSDQNQNHPSLTSLGDYFIGPGLDLLLQHLAENDPNRQGTPPARKEAVEALPTVKIMEPL 257

Query: 202 LNSEMNQCAVCIDGFEKGIQVKQMPCKHVFHDYCILPWLELHNSCPVCRFELPTD-DSDY 261
                 QC+VC+D FEKG + K+MPCKH FH  CI+PWLELH+SCPVCRFELP+  D D 
Sbjct: 258 ------QCSVCLDDFEKGTEAKEMPCKHKFHVRCIVPWLELHSSCPVCRFELPSSADDDD 317

Query: 262 ENRTRGRRV----QANESSGEESGSGHGGSSRERVE-------RRFRVPLWWPFGGRNDG 320
           E +T   RV       E+S        G + R R +       RRF  P  WPF G    
Sbjct: 318 ETKTDSERVLRTRNVRETSNGNVVENVGNADRGREDEVRSGNGRRFSFP--WPFSGLFSS 377

[Close]

BLAST of Lag0029407.1 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 157.9 bits (398), Expect = 1.4e-38
Identity = 115/335 (34.33%), Postives = 161/335 (48.06%), Query Frame = 0


Query: 1   MSSASNLAADKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEE--------YENFSSNS 60
           MSS+ N       +CH+C RAV +      +P+C +C GGF+EE        ++ F S+ 
Sbjct: 1   MSSSRN-----THWCHRCQRAVRL---HGQEPVCFYCGGGFVEELDMAQASPFDMFRSHR 60

Query: 61  NANLDPRSFFLPDPFRSFNPFVFSSSRVVDLQNP-GLFSRTLSPAPQPWNRHQSDSSNQS 120
                 ++F L D F      VF  +R+ +  +   +  RT+S  P+ +           
Sbjct: 61  GVVERDQTFDLMDAFS-----VFMRNRLAERSHDREIRGRTISSGPENF----------- 120

Query: 121 EPFDPFVFL--QNHLRGIFDSGADVVFEIQGNPSHSGVSMPQ-NVGDYFIGPGLEQLIQL 180
               P +    Q   R   D+  + +F    N    G+ + + N GDYF GPGLE+L + 
Sbjct: 121 PGLAPLLIFGGQVPYRLTGDNAVEALF----NGGSPGIGITRGNTGDYFFGPGLEELFEQ 180

Query: 181 LAENDPNRYGTPPASKSAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHV 240
           L+     R G PPA +SAI+ LPT+ +    L S  + C VC D FE G + KQMPC H+
Sbjct: 181 LSAGTTRR-GPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHI 240

Query: 241 FHDYCILPWLELHNSCPVCRFELP--TDDSDYENRTRGRRVQANESSGEESGSGHGGSSR 300
           +H  CI+PWL  HNSCPVCR ELP  +  S  +NRT   R   + SS   S S   G+  
Sbjct: 241 YHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRENGN-- 300

Query: 301 ERVERRFRVPLWWPFGGRNDGSDS----GGGNDSD 318
              ERR     +WPF      S S    GG  +SD
Sbjct: 301 ---ERRNPFSSFWPFRSSGSSSSSTQNRGGTRNSD 301

[Close]

BLAST of Lag0029407.1 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 157.9 bits (398), Expect = 1.4e-38
Identity = 115/335 (34.33%), Postives = 161/335 (48.06%), Query Frame = 0


Query: 1   MSSASNLAADKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEE--------YENFSSNS 60
           MSS+ N       +CH+C RAV +      +P+C +C GGF+EE        ++ F S+ 
Sbjct: 1   MSSSRN-----THWCHRCQRAVRL---HGQEPVCFYCGGGFVEELDMAQASPFDMFRSHR 60

Query: 61  NANLDPRSFFLPDPFRSFNPFVFSSSRVVDLQNP-GLFSRTLSPAPQPWNRHQSDSSNQS 120
                 ++F L D F      VF  +R+ +  +   +  RT+S  P+ +           
Sbjct: 61  GVVERDQTFDLMDAFS-----VFMRNRLAERSHDREIRGRTISSGPENF----------- 120

Query: 121 EPFDPFVFL--QNHLRGIFDSGADVVFEIQGNPSHSGVSMPQ-NVGDYFIGPGLEQLIQL 180
               P +    Q   R   D+  + +F    N    G+ + + N GDYF GPGLE+L + 
Sbjct: 121 PGLAPLLIFGGQVPYRLTGDNAVEALF----NGGSPGIGITRGNTGDYFFGPGLEELFEQ 180

Query: 181 LAENDPNRYGTPPASKSAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHV 240
           L+     R G PPA +SAI+ LPT+ +    L S  + C VC D FE G + KQMPC H+
Sbjct: 181 LSAGTTRR-GPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHI 240

Query: 241 FHDYCILPWLELHNSCPVCRFELP--TDDSDYENRTRGRRVQANESSGEESGSGHGGSSR 300
           +H  CI+PWL  HNSCPVCR ELP  +  S  +NRT   R   + SS   S S   G+  
Sbjct: 241 YHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRENGN-- 300

Query: 301 ERVERRFRVPLWWPFGGRNDGSDS----GGGNDSD 318
              ERR     +WPF      S S    GG  +SD
Sbjct: 301 ---ERRNPFSSFWPFRSSGSSSSSTQNRGGTRNSD 301

[Close]

BLAST of Lag0029407.1 vs. TAIR 10
Match: AT2G40830.3 (RING-H2 finger C1A )

HSP 1 Score: 157.9 bits (398), Expect = 1.4e-38
Identity = 115/335 (34.33%), Postives = 161/335 (48.06%), Query Frame = 0


Query: 1   MSSASNLAADKPFFCHQCDRAVTVTVSTSSDPLCPFCNGGFLEE--------YENFSSNS 60
           MSS+ N       +CH+C RAV +      +P+C +C GGF+EE        ++ F S+ 
Sbjct: 1   MSSSRN-----THWCHRCQRAVRL---HGQEPVCFYCGGGFVEELDMAQASPFDMFRSHR 60

Query: 61  NANLDPRSFFLPDPFRSFNPFVFSSSRVVDLQNP-GLFSRTLSPAPQPWNRHQSDSSNQS 120
                 ++F L D F      VF  +R+ +  +   +  RT+S  P+ +           
Sbjct: 61  GVVERDQTFDLMDAFS-----VFMRNRLAERSHDREIRGRTISSGPENF----------- 120

Query: 121 EPFDPFVFL--QNHLRGIFDSGADVVFEIQGNPSHSGVSMPQ-NVGDYFIGPGLEQLIQL 180
               P +    Q   R   D+  + +F    N    G+ + + N GDYF GPGLE+L + 
Sbjct: 121 PGLAPLLIFGGQVPYRLTGDNAVEALF----NGGSPGIGITRGNTGDYFFGPGLEELFEQ 180

Query: 181 LAENDPNRYGTPPASKSAIERLPTVSVCDDLLNSEMNQCAVCIDGFEKGIQVKQMPCKHV 240
           L+     R G PPA +SAI+ LPT+ +    L S  + C VC D FE G + KQMPC H+
Sbjct: 181 LSAGTTRR-GPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHI 240

Query: 241 FHDYCILPWLELHNSCPVCRFELP--TDDSDYENRTRGRRVQANESSGEESGSGHGGSSR 300
           +H  CI+PWL  HNSCPVCR ELP  +  S  +NRT   R   + SS   S S   G+  
Sbjct: 241 YHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRENGN-- 300

Query: 301 ERVERRFRVPLWWPFGGRNDGSDS----GGGNDSD 318
              ERR     +WPF      S S    GG  +SD
Sbjct: 301 ---ERRNPFSSFWPFRSSGSSSSSTQNRGGTRNSD 301

The following BLAST results are available for this feature:

BLAST of Lag0029407.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023554317.1	2.3e-157	85.22	E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucurbita pepo subsp. pepo] >...	[more]
XP_022960730.1	2.3e-157	84.91	E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucurbita moschata]	[more]
KAG7032683.1	5.1e-157	84.91	E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6601985.1	5.1e-157	84.91	E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022990139.1	5.7e-156	84.91	E3 ubiquitin-protein ligase RING1-like isoform X1 [Cucurbita maxima]	[more]

BLAST of Lag0029407.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPN7	3.3e-90	54.63	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	1.5e-87	52.58	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
O22197	1.9e-37	34.33	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q94AK4	4.0e-35	33.33	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]
Q6AVN2	1.7e-33	33.22	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]

BLAST of Lag0029407.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H9Y0	1.1e-157	84.91	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461423...	[more]
A0A6J1JR94	2.8e-156	84.91	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111487122 P...	[more]
A0A6J1CK28	5.4e-152	80.61	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101187...	[more]
A0A6J1FJU6	6.1e-148	82.91	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111444684...	[more]
A0A6J1K1C4	4.4e-146	81.65	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111489246 P...	[more]

BLAST of Lag0029407.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19950.1	2.3e-91	54.63	RING/U-box superfamily protein	[more]
AT5G56340.1	7.2e-40	47.92	RING/U-box superfamily protein	[more]
AT2G40830.2	1.4e-38	34.33	RING-H2 finger C1A	[more]
AT2G40830.1	1.4e-38	34.33	RING-H2 finger C1A	[more]
AT2G40830.3	1.4e-38	34.33	RING-H2 finger C1A	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 207..247 e-value: 2.1E-6 score: 37.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 207..248 score: 12.326995
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 170..254 e-value: 2.0E-21 score: 77.5
None	No IPR available	PFAM	PF13923	zf-C3HC4_2	coord: 206..247 e-value: 1.3E-12 score: 47.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 255..270
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 255..322
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 92..111
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 8..296
None	No IPR available	PANTHER	PTHR15710:SF75	E3 UBIQUITIN-PROTEIN LIGASE RING1	coord: 8..296
None	No IPR available	CDD	cd16667	RING-H2_RNF126_like	coord: 206..248 e-value: 1.54313E-21 score: 83.9064
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 186..256
IPR039525	E3 ubiquitin-protein ligase RNF126-like, zinc-ribbon	PFAM	PF14369	zinc_ribbon_9	coord: 13..45 e-value: 1.4E-14 score: 54.1

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0029407	Lag0029407	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0029407.1.exon1	Lag0029407.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0029407.1	cds.Lag0029407.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0029407.1	Lag0029407.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity