Lag0029380.1 (mRNA) Sponge gourd (AG‐4) v1

BLAST of Lag0029380.1 vs. NCBI nr
Match: XP_022923630.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita moschata])

HSP 1 Score: 1005.7 bits (2599), Expect = 1.6e-289
Identity = 492/576 (85.42%), Postives = 527/576 (91.49%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           M+ LSKCDANYA LTPITFLKRASA Y+DRTSIIYEGTRFTWRQTY+RC RLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           V KN VVSV+APN+PALYEMHFAVPMAGAILNTINTRLD+ NIA+ILRHSEAK+ FVDY+
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFVSMEVDD 180
           +IQ+AK+AL  L A S   LP VVVIDDIDSPTGAR G LEYEQL+H GDVGF  +EVDD
Sbjct: 121 FIQEAKDALRQLMAAS-LPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDD 180

Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
           EWDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240

Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIGSIVN 300
           TWGIAARGGTNVCMRNTTAFDI+RNI+LHHVTHMCCAPIVFRIIL+A+ G  +R G +VN
Sbjct: 241 TWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVN 300

Query: 301 VMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKLKARQ 360
           V+TGGAPPPA LLEKMEALGF+ITHAYGLTEATGP LVCEWQEKWN+LPADEQAKLKARQ
Sbjct: 301 VLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQ 360

Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
           GISILTLADVDVKNL+TM+SVPHDGRT+GEIVLRGSSIMKGYFKDPAATA AF+NGWFLT
Sbjct: 361 GISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLT 420

Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGES 480
           GDVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGES
Sbjct: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGES 480

Query: 481 PCAFVAVH---GGVSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAK 540
           PCAFV VH   G VSEAE+I FCRKN+SHYM PKKVVFVAELPKT TGKIQKFKLREQAK
Sbjct: 481 PCAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAK 540

Query: 541 LFNVSEKEVAGGNKSDQTDGGRQSGYDEHVLTRSRL 574
           L   SEK V+GGNKS + DGGRQ  Y +HV+  SRL
Sbjct: 541 LLVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 575

BLAST of Lag0029380.1 vs. NCBI nr
Match: XP_023513711.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1005.0 bits (2597), Expect = 2.6e-289
Identity = 492/576 (85.42%), Postives = 527/576 (91.49%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           M+ LSKCDANYA LTPITFLKRASA Y+DRTSIIYEGTRFTWRQTYERC RLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLSRLN 60

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           V KN VVSV+APN+PALYEMHFAVPMAGAILNTINTRLD+ NIA+ILRHSEAK+ FVDY+
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFVSMEVDD 180
           +IQ+AK+AL  L A S   LP VVVIDDIDSPTGAR G LEYEQL+H GDVGF  +EVDD
Sbjct: 121 FIQEAKDALRQLMAAS-LPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDD 180

Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
           EWDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLV+GWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVVGWEMGNAPVYLWTLPMFHCNGWTF 240

Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIGSIVN 300
           TWGIAARGGTNVCMRNTTAFDI+RNI+LHHVTHMCCAPIVFRIIL+A+ G  +R G +VN
Sbjct: 241 TWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVN 300

Query: 301 VMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKLKARQ 360
           V+TGGAPPPA LLEKMEALGF+ITHAYGLTEATGP LVCEWQEKWN+LPADEQAKLKARQ
Sbjct: 301 VLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQ 360

Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
           GISILTLADVDVKNL+TM+SVPHDGRT+GEIVLRGSSIMKGYFKDPAATA AF+NGWFLT
Sbjct: 361 GISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLT 420

Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGES 480
           GDVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGES
Sbjct: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGES 480

Query: 481 PCAFVAVH---GGVSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAK 540
           PCAFV VH   G VSEAE+I FCRKN+SHYM PKKVVFVAELPKT TGKIQKFKLREQAK
Sbjct: 481 PCAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAK 540

Query: 541 LFNVSEKEVAGGNKSDQTDGGRQSGYDEHVLTRSRL 574
           L   SEK V+GGNKS + DGGRQ  Y +HV+  SRL
Sbjct: 541 LLVGSEKRVSGGNKSGRHDGGRQPEYGQHVMAMSRL 575

BLAST of Lag0029380.1 vs. NCBI nr
Match: KAG6602004.1 (Benzoate--CoA ligase, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1001.9 bits (2589), Expect = 2.2e-288
Identity = 491/576 (85.24%), Postives = 526/576 (91.32%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           M+ LSKCDANYA LTPITFLKRASA Y+DRTSIIYEGTRFTWRQTY+RC RLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           V KN VVSV+APN+PALYEMHFAVPMAGAILNTINTRLD+ NIA+ILRHSEAK+ FVDY+
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFVSMEVDD 180
           +IQ+AK+AL  L A S   LP VVVIDDIDSPTGAR G LEYEQL+H GDVGF  +EVDD
Sbjct: 121 FIQEAKDALRQLMAAS-LPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDD 180

Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
           EWDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240

Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIGSIVN 300
           TWGIAARGGTNVCMRNTTAFDI+RNI+LHHVTHMCCAPIVFRIIL+A+ G  +R G +VN
Sbjct: 241 TWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVN 300

Query: 301 VMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKLKARQ 360
           V+TGGAPPPA LLEKMEALGF+ITHAYGLTEATGP LVCEWQEKWN+LPADEQAKLKARQ
Sbjct: 301 VLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQ 360

Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
           GISILTLADVDVKNL+TM+SVPHDGRT+GEIVLRGSSIMKGYFKD AATA AF+NGWFLT
Sbjct: 361 GISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDLAATAKAFQNGWFLT 420

Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGES 480
           GDVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGES
Sbjct: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGES 480

Query: 481 PCAFVAVH---GGVSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAK 540
           PCAFV VH   G VSEAE+I FCRKN+SHYM PKKVVFVAELPKT TGKIQKFKLREQAK
Sbjct: 481 PCAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAK 540

Query: 541 LFNVSEKEVAGGNKSDQTDGGRQSGYDEHVLTRSRL 574
           L   SEK V+GGNKS + DGGRQ  Y +HV+  SRL
Sbjct: 541 LLVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 575

BLAST of Lag0029380.1 vs. NCBI nr
Match: XP_022990938.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita maxima])

HSP 1 Score: 997.7 bits (2578), Expect = 4.2e-287
Identity = 488/576 (84.72%), Postives = 524/576 (90.97%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           M+ LSKCDAN+A LTPITFLKRASA Y+DRTSIIYEGT FTWRQTYERC  LASSL RLN
Sbjct: 1   MNSLSKCDANFASLTPITFLKRASAFYADRTSIIYEGTSFTWRQTYERCCHLASSLSRLN 60

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           V KN VVSV+APN+PALYEMHFAVPMAGAILNTINTRLD+ NIA+ILRHSEAK+ FVDY+
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFVSMEVDD 180
           +IQ+AK+AL  L A S   LP VVVIDDIDSPTGAR G LEYEQL+H GDVGF  +EVDD
Sbjct: 121 FIQEAKDALRQLMAAS-LPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDD 180

Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
           EWDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240

Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIGSIVN 300
           TWGIAARGGTNVC+RNTTAFDI+RNI+LHHVTHMCCAPIVFRIIL+A+ G  +R G +VN
Sbjct: 241 TWGIAARGGTNVCIRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVN 300

Query: 301 VMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKLKARQ 360
           V+TGGAPPPA LLEKMEALGF+ITHAYGLTEATGP LVCEWQEKWN+LPADEQAKLKARQ
Sbjct: 301 VLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQ 360

Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
           GISILTLADVDVKNL+TM+SVPHDGRT+GEIVLRGSSIMKGYFKDPAATA AF+NGWFLT
Sbjct: 361 GISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLT 420

Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGES 480
           GDVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGES
Sbjct: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGES 480

Query: 481 PCAFVAVH---GGVSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAK 540
           PCAFV VH   G VSEAE+I FCRKN+SHYM PKKV+FVAELPKT TGKIQKFKLREQAK
Sbjct: 481 PCAFVVVHSDSGKVSEAEMIEFCRKNMSHYMAPKKVLFVAELPKTLTGKIQKFKLREQAK 540

Query: 541 LFNVSEKEVAGGNKSDQTDGGRQSGYDEHVLTRSRL 574
           L   SEK V+GGNKS + DGGR   Y EHV+  SRL
Sbjct: 541 LLVASEKRVSGGNKSGRHDGGRLPEYGEHVMAMSRL 575

BLAST of Lag0029380.1 vs. NCBI nr
Match: XP_011655134.1 (butyrate--CoA ligase AAE11, peroxisomal [Cucumis sativus] >KGN51049.2 hypothetical protein Csa_023114 [Cucumis sativus])

HSP 1 Score: 976.5 bits (2523), Expect = 1.0e-280
Identity = 482/580 (83.10%), Postives = 524/580 (90.34%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           M+ L KCDANY+ LTPITFLKRASA Y+DRTSIIYEGTRFTW+QTYERC RLASSLCRL 
Sbjct: 1   MEMLLKCDANYSALTPITFLKRASAFYADRTSIIYEGTRFTWKQTYERCCRLASSLCRLT 60

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           V+KNDVVSV+APN+PALYEMHFAVPMAGAILNTINTRLDIKNIA+ILRHSEAK+FFVDY+
Sbjct: 61  VSKNDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDIKNIALILRHSEAKIFFVDYQ 120

Query: 121 YIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFVSMEVDD 180
           YIQ+AK+AL +L AES S +PLVVVIDDID+PTGARLG+LEYEQLI+ GD  F  +EVDD
Sbjct: 121 YIQEAKDALRLLVAES-SPIPLVVVIDDIDTPTGARLGNLEYEQLIYDGDANFTPVEVDD 180

Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
           EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240

Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIGSIVN 300
           TWGIAARGGTN+CMRNTTA DIFRNI+LH VTHMCCAPIVF IIL+ADN   +R+   VN
Sbjct: 241 TWGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFTIILEADNRDRRRVAWPVN 300

Query: 301 VMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKLKARQ 360
           V+TGGAPPPAPLLEKMEALGF+ITHAYGLTEATGP LVCEWQEKWNVLPAD+QA LKARQ
Sbjct: 301 VLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQ 360

Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
           GISILTLADVDVKNL TMESVPHDGRT+GEIVLRGSS+MKGYFKDPAATAAA +NGWFLT
Sbjct: 361 GISILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPAATAAALKNGWFLT 420

Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGES 480
           GD+GVI+PDGY+EIKDRSKDVIISGGENISSVE+ETVLYRHPQV+EAAVVAMPHPRWGES
Sbjct: 421 GDIGVIYPDGYVEIKDRSKDVIISGGENISSVEVETVLYRHPQVSEAAVVAMPHPRWGES 480

Query: 481 PCAFVAVHGG-----VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQ 540
           PCAFV +  G     VSEAE++AFCRKNLSHYMVPKKVVFVAELPK + GK+QKF LREQ
Sbjct: 481 PCAFVVLQDGGGSGRVSEAEMVAFCRKNLSHYMVPKKVVFVAELPK-ANGKVQKFMLREQ 540

Query: 541 AKLFNVSEKEVAG--GNKSDQTDGGRQSGYDEHVLTRSRL 574
           AK   VSEK +    GNKS    G R+S Y EHV+  SRL
Sbjct: 541 AKYLEVSEKRIVSNMGNKS----GNRRSDYGEHVIAMSRL 574

BLAST of Lag0029380.1 vs. ExPASy Swiss-Prot
Match: I3PB36 (Trans-cinnamate:CoA ligase, peroxisomal OS=Petunia hybrida OX=4102 GN=CNL PE=1 SV=1)

HSP 1 Score: 745.0 bits (1922), Expect = 6.5e-214
Identity = 363/553 (65.64%), Postives = 431/553 (77.94%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           MD+L KC ANY PLTP+TFL RA  SY++RTSIIY G RFTW QTY+RC RLASSL  LN
Sbjct: 1   MDELPKCGANYVPLTPLTFLTRAFKSYANRTSIIYAGARFTWEQTYKRCCRLASSLQSLN 60

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           + KNDVVSV+APN+PA YEMHFAVPMAGA+LNTINTRLD  NIAIIL+HSEAKL FVDYE
Sbjct: 61  IVKNDVVSVLAPNVPATYEMHFAVPMAGAVLNTINTRLDPMNIAIILKHSEAKLLFVDYE 120

Query: 121 YIQQAKEALHVLAAESPST--------LPLVVVIDDIDSPTG-ARLGSLEYEQLIHRGDV 180
           Y+++A++AL +L + +  T        +P V++IDD+ SPT   +   LEYEQL+H+G+ 
Sbjct: 121 YLEKARKALELLMSTNFITAQNSKKISMPQVILIDDLYSPTRIQQQDQLEYEQLVHQGNP 180

Query: 181 GFVSME-VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTL 240
            +     VDDEWD I LNYTSGTTS PKGVVYSHRGAFLSTL+ +MGWEMG  PVYLW+L
Sbjct: 181 EYAPENIVDDEWDPIVLNYTSGTTSEPKGVVYSHRGAFLSTLNTIMGWEMGTEPVYLWSL 240

Query: 241 PMFHCNGWTFTWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNG 300
           PMFH NGWT TWGIAARGGTNVC+RNTTA +I+ NI LH VTHMCCAP VF I+L+A   
Sbjct: 241 PMFHINGWTLTWGIAARGGTNVCIRNTTAQEIYSNITLHKVTHMCCAPTVFNILLEAKPH 300

Query: 301 SPKRIGSIVNVMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPA 360
             + I + V VM GGAPPP  L+ K+E LGF++ H YG+TEA G  LVCEWQ +WN L  
Sbjct: 301 ERREITTPVQVMVGGAPPPTTLIGKIEELGFHVVHCYGITEAGGTTLVCEWQSEWNKLSR 360

Query: 361 DEQAKLKARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATA 420
           ++QA LKARQGIS+L L DVDVKN  TM+SVPH+G+T GEI LRGSSIMKGYFK+  A +
Sbjct: 361 EDQANLKARQGISVLALEDVDVKNSKTMQSVPHNGKTMGEICLRGSSIMKGYFKNDKANS 420

Query: 421 AAFRNGWFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVV 480
             F+NGWFLTGDV VIH DGYLEIKDR KD+IISGGENISS+E+E  + +HP V EAAVV
Sbjct: 421 QVFKNGWFLTGDVAVIHQDGYLEIKDRCKDIIISGGENISSIEVENAILKHPSVIEAAVV 480

Query: 481 AMPHPRWGESPCAFV--AVHGGVSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQ 540
           AMPHPRWGE+PCAFV    +  + EA+II  C+K L  +MVPK V F+ ELPKT TGK++
Sbjct: 481 AMPHPRWGETPCAFVIKTKNPEIKEADIIVHCKKELPGFMVPKHVQFLEELPKTGTGKVK 540

Query: 541 KFKLREQAKLFNV 542
           K +LRE AK F +
Sbjct: 541 KLQLREMAKSFGI 553

BLAST of Lag0029380.1 vs. ExPASy Swiss-Prot
Match: Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 7.7e-199
Identity = 340/563 (60.39%), Postives = 421/563 (74.78%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           MD L  C+AN  PLTPITFLKRAS  Y +RTSIIY  TRFTW QTY+RC RLA+SL  LN
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           +T+NDVVS++APN+PA+YEMHF+VPM GA+LN INTRLD K IAIILRH+E K+ FVDYE
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 YIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFVS----M 180
           +    +E L ++        P +++I++IDS T      L+YE LI +G+    S     
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASMF 180

Query: 181 EVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            V +E D I+LNYTSGTT+ PKGVV SH+GA+LS LS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIG 300
           GWT TW +AARGGTNVC+R+ TA +I++NI+LH VTHM C P VFR +L+          
Sbjct: 241 GWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKS 300

Query: 301 SIVNVMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKL 360
           S V V+TGG+ PPA L++K+E LGF++ H YGLTEATGP L CEWQ++WN LP  +Q +L
Sbjct: 301 SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIEL 360

Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNG 420
           + RQG+  LTLADVDVKN  T+ESVP DG+T GEIV++GSS+MKGY K+P AT+ AF++G
Sbjct: 361 QQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  TGD+GVIHPDGY+EIKDRSKD+IISGGENISS+E+E VLY + +V EAAVVAMPHP 
Sbjct: 421 WLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMPHPL 480

Query: 481 WGESPCAFVAVHGG-----VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFK 540
           WGE+PCAFV +  G      SE ++I +CR+N+ H+M PKKVVF  ELPK S GKI K K
Sbjct: 481 WGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILKSK 540

Query: 541 LREQAKLFNVSEKEVAGGNKSDQ 555
           LR+ AK   V E + AG  K  Q
Sbjct: 541 LRDIAKALVVREDD-AGSKKVHQ 562

BLAST of Lag0029380.1 vs. ExPASy Swiss-Prot
Match: Q9SS01 (Benzoate--CoA ligase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE20 PE=1 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 5.5e-197
Identity = 341/569 (59.93%), Postives = 423/569 (74.34%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           MD L+ C+AN  PLTP+TFLKRAS  Y +RTSIIY  TRFTW QTY+RC RLA+SL  LN
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           ++KNDVVSV+APN PALYEMHFAVPMAGA+LN INTRLD  +IA ILRH++ K+ F+D  
Sbjct: 61  ISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRS 120

Query: 121 YIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGD----VGFVSM 180
           +   A+E+LH+L++E  +    V+ I + D P  A    L+YE LI RG+    +     
Sbjct: 121 FEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLIQRGEPTPSMVARMF 180

Query: 181 EVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            + DE D I+LNYTSGTT+ PKGVV SHRGA+L TLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIG 300
           GWTFTWG AARGGT+VCMR+ TA +I++NI++H+VTHMCC P VF I+L  ++       
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRS 300

Query: 301 SIVNVMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKL 360
             V+V+TGG+PPPA L++K++ LGF + HAYG TEATGP L CEWQ++WN LP ++Q +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNG 420
           KARQGISIL LADVDVKN +T +S P DG+T GEI+++GSSIMKGY K+P AT  AF++G
Sbjct: 361 KARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKATFEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  TGDVGVIHPDG++EIKDRSKD+IISGGENISSVE+E VLY++P+V E AVVAMPHP 
Sbjct: 421 WLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFVAVHGG-------------VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTS 540
           WGE+PCAFV +                  E  +I +CR+NL H+M P+KVVF+ ELPK  
Sbjct: 481 WGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKNG 540

Query: 541 TGKIQKFKLREQAK------LFNVSEKEV 547
            GKI K KLR+ AK        NV  KEV
Sbjct: 541 NGKILKPKLRDIAKGLVVEDEINVIAKEV 569

BLAST of Lag0029380.1 vs. ExPASy Swiss-Prot
Match: Q9SS00 (Probable acyl-activating enzyme 12, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE12 PE=2 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 2.7e-196
Identity = 336/562 (59.79%), Postives = 423/562 (75.27%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           MD L+ C+AN  PLTPITFLKRAS  Y +RTSIIY  TRFTW QTY+RC RLA+SL  LN
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           + KNDVVSV+APN PA+YEMHFAVPMAGA+LN INTRLD  +IA ILRH++ K+ F+   
Sbjct: 61  IGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRS 120

Query: 121 YIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFVSME--- 180
           +   A+E L +L++E  +    V+ I +ID P        +YE LI RG+   + +    
Sbjct: 121 FEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQRGEPTPLLLARMF 180

Query: 181 -VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            + DE D I+LNYTSGTT+ PKGVV SHRGA+LSTLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 CIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIG 300
           GWTFTWG AARGGT+VCMR+ TA +I++NI++H+VTHMCC P VF I+L  ++       
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSHRS 300

Query: 301 SIVNVMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKL 360
             V+V+TGG+PPPA L++K++ LGF + HAYGLTEATGP L CEWQ++WN LP ++Q +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNG 420
           KARQG+SIL L +VDV+N +T ESVP DG+T GEIV++GSSIMKGY K+P AT  AF++G
Sbjct: 361 KARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  +GDVGVIHPDG++EIKDRSKD+IISGGENISSVE+E ++Y++P+V E AVVAMPHP 
Sbjct: 421 WLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFVAVHGG------------VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTST 540
           WGE+PCAFV +  G              E ++I +CR+NL H+M P+KVVF+ ELPK   
Sbjct: 481 WGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPKNGN 540

Query: 541 GKIQKFKLREQAKLFNVSEKEV 547
           GKI K KLR+ AK   V+E EV
Sbjct: 541 GKILKPKLRDIAKGL-VAEDEV 561

BLAST of Lag0029380.1 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 6.6e-166
Identity = 283/550 (51.45%), Postives = 390/550 (70.91%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           M+   K  ANY PLTPI+FL R++  Y+DR SI+Y   ++TWRQT +RC R+AS+L +L 
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           ++  DVVSV+APN+PA+ E+HF VPMAGA+L T+N R D   +A++LRHS  K+ F D++
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 YIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGS---LEYEQLIHRGDVGFVSME 180
           ++Q A+ A  +L+ +      LV++ + +      +  S   +EYE ++  G   F  + 
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 181 VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNG 240
             DE D+I++NYTSGTTS+PKGVVYSHRGA+L++L+ V+  EM ++P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 241 WTFTWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIGS 300
           W   WG+ A GGTN+C+RN TA  IF NI  H VTHM  AP +  +I++A     K +  
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 301 IVNVMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKLK 360
            V+ +TG APPPA ++ KME LGF + H+YGLTE  GPG +C W+ +W+ LP +EQAK+K
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

Query: 361 ARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGW 420
           ARQG++ L L ++ VK+  TM ++P DG T GE+V RG+++M GY K+P AT  AF+ GW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422

Query: 421 FLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRW 480
           F +GD+GV HPDGY+E+KDRSKD+IISGGENISS+E+E+ L+ HP V EAAVVA P   W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482

Query: 481 GESPCAFVAVHGG--VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQ 540
           GE+ CAFV +  G   S  E+I++CR  L HYM P+ +VF  +LPKTSTGK+QKF LR +
Sbjct: 483 GETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVF-EDLPKTSTGKVQKFVLRTK 542

Query: 541 AK-LFNVSEK 545
           AK L ++S+K
Sbjct: 543 AKALVSLSKK 551

BLAST of Lag0029380.1 vs. ExPASy TrEMBL
Match: A0A6J1ECD9 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111431279 PE=4 SV=1)

HSP 1 Score: 1005.7 bits (2599), Expect = 7.5e-290
Identity = 492/576 (85.42%), Postives = 527/576 (91.49%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           M+ LSKCDANYA LTPITFLKRASA Y+DRTSIIYEGTRFTWRQTY+RC RLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           V KN VVSV+APN+PALYEMHFAVPMAGAILNTINTRLD+ NIA+ILRHSEAK+ FVDY+
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFVSMEVDD 180
           +IQ+AK+AL  L A S   LP VVVIDDIDSPTGAR G LEYEQL+H GDVGF  +EVDD
Sbjct: 121 FIQEAKDALRQLMAAS-LPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDD 180

Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
           EWDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240

Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIGSIVN 300
           TWGIAARGGTNVCMRNTTAFDI+RNI+LHHVTHMCCAPIVFRIIL+A+ G  +R G +VN
Sbjct: 241 TWGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVN 300

Query: 301 VMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKLKARQ 360
           V+TGGAPPPA LLEKMEALGF+ITHAYGLTEATGP LVCEWQEKWN+LPADEQAKLKARQ
Sbjct: 301 VLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQ 360

Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
           GISILTLADVDVKNL+TM+SVPHDGRT+GEIVLRGSSIMKGYFKDPAATA AF+NGWFLT
Sbjct: 361 GISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLT 420

Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGES 480
           GDVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGES
Sbjct: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGES 480

Query: 481 PCAFVAVH---GGVSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAK 540
           PCAFV VH   G VSEAE+I FCRKN+SHYM PKKVVFVAELPKT TGKIQKFKLREQAK
Sbjct: 481 PCAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAK 540

Query: 541 LFNVSEKEVAGGNKSDQTDGGRQSGYDEHVLTRSRL 574
           L   SEK V+GGNKS + DGGRQ  Y +HV+  SRL
Sbjct: 541 LLVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 575

BLAST of Lag0029380.1 vs. ExPASy TrEMBL
Match: A0A6J1JUR4 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111487678 PE=4 SV=1)

HSP 1 Score: 997.7 bits (2578), Expect = 2.0e-287
Identity = 488/576 (84.72%), Postives = 524/576 (90.97%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           M+ LSKCDAN+A LTPITFLKRASA Y+DRTSIIYEGT FTWRQTYERC  LASSL RLN
Sbjct: 1   MNSLSKCDANFASLTPITFLKRASAFYADRTSIIYEGTSFTWRQTYERCCHLASSLSRLN 60

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           V KN VVSV+APN+PALYEMHFAVPMAGAILNTINTRLD+ NIA+ILRHSEAK+ FVDY+
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFVSMEVDD 180
           +IQ+AK+AL  L A S   LP VVVIDDIDSPTGAR G LEYEQL+H GDVGF  +EVDD
Sbjct: 121 FIQEAKDALRQLMAAS-LPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDD 180

Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
           EWDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240

Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIGSIVN 300
           TWGIAARGGTNVC+RNTTAFDI+RNI+LHHVTHMCCAPIVFRIIL+A+ G  +R G +VN
Sbjct: 241 TWGIAARGGTNVCIRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVN 300

Query: 301 VMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKLKARQ 360
           V+TGGAPPPA LLEKMEALGF+ITHAYGLTEATGP LVCEWQEKWN+LPADEQAKLKARQ
Sbjct: 301 VLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQ 360

Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
           GISILTLADVDVKNL+TM+SVPHDGRT+GEIVLRGSSIMKGYFKDPAATA AF+NGWFLT
Sbjct: 361 GISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLT 420

Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGES 480
           GDVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGES
Sbjct: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGES 480

Query: 481 PCAFVAVH---GGVSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAK 540
           PCAFV VH   G VSEAE+I FCRKN+SHYM PKKV+FVAELPKT TGKIQKFKLREQAK
Sbjct: 481 PCAFVVVHSDSGKVSEAEMIEFCRKNMSHYMAPKKVLFVAELPKTLTGKIQKFKLREQAK 540

Query: 541 LFNVSEKEVAGGNKSDQTDGGRQSGYDEHVLTRSRL 574
           L   SEK V+GGNKS + DGGR   Y EHV+  SRL
Sbjct: 541 LLVASEKRVSGGNKSGRHDGGRLPEYGEHVMAMSRL 575

BLAST of Lag0029380.1 vs. ExPASy TrEMBL
Match: A0A5A7TXW0 (Butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold284G00160 PE=4 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 1.4e-280
Identity = 485/581 (83.48%), Postives = 524/581 (90.19%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           M+ L KCDANY  LTPITFLKRASA Y+DRTSIIYEGTRFTW QTYERC RLASSL RL+
Sbjct: 1   MEMLLKCDANYTALTPITFLKRASAFYADRTSIIYEGTRFTWNQTYERCCRLASSLRRLS 60

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           V+KN VVSV+APN+PALYEMHFAVPMAGAILNTINTRLD+KNIA+ILRHSEAK+FFVDY+
Sbjct: 61  VSKNHVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVKNIALILRHSEAKIFFVDYQ 120

Query: 121 YIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFVSMEVDD 180
           YIQ+AK+AL +L AES  T+PLVVVIDDID+PTGARLG+LEYEQLIH GD  F  +EVDD
Sbjct: 121 YIQEAKDALRLLVAES-FTIPLVVVIDDIDTPTGARLGNLEYEQLIHDGDANFTPVEVDD 180

Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
           EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240

Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIGSIVN 300
           TWGIAARGGTN+CMRNTTA DIFRNI+LH VTHMCCAPIVFRIIL+ADNG  +R+   VN
Sbjct: 241 TWGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFRIILEADNGDRRRVAWPVN 300

Query: 301 VMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKLKARQ 360
           V+TGGAPPPA LLEKMEALGF+ITHAYGLTEATGP LVCEWQEKWNVLPAD+QA LKARQ
Sbjct: 301 VLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQ 360

Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
           GISILTLADVDVKNL TMESVPHDGRT+GEIVLRGSS+MKGYFKDP ATAAAF+NGWFLT
Sbjct: 361 GISILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPEATAAAFKNGWFLT 420

Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGES 480
           GD+GVI+PDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGES
Sbjct: 421 GDIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGES 480

Query: 481 PCAFVAVHGG-----VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQ 540
           PCAFV +HGG     VSEAE+IAFCRKNLSHYMVPKKVVF+AELPK + GK+QKF LREQ
Sbjct: 481 PCAFVVLHGGGGSGRVSEAEMIAFCRKNLSHYMVPKKVVFMAELPK-ANGKVQKFMLREQ 540

Query: 541 AKLFNVSEKEVAG--GNKSDQTDGGRQSGY-DEHVLTRSRL 574
           AK   VSEK      GNKS    G R+S Y D+HV+  SRL
Sbjct: 541 AKYLEVSEKRRVSNLGNKS----GNRRSDYGDQHVIAMSRL 575

BLAST of Lag0029380.1 vs. ExPASy TrEMBL
Match: A0A1S3CIM1 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103501376 PE=4 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 1.4e-280
Identity = 485/581 (83.48%), Postives = 524/581 (90.19%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           M+ L KCDANY  LTPITFLKRASA Y+DRTSIIYEGTRFTW QTYERC RLASSL RL+
Sbjct: 1   MEMLLKCDANYTALTPITFLKRASAFYADRTSIIYEGTRFTWNQTYERCCRLASSLRRLS 60

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           V+KN VVSV+APN+PALYEMHFAVPMAGAILNTINTRLD+KNIA+ILRHSEAK+FFVDY+
Sbjct: 61  VSKNHVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVKNIALILRHSEAKIFFVDYQ 120

Query: 121 YIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFVSMEVDD 180
           YIQ+AK+AL +L AES  T+PLVVVIDDID+PTGARLG+LEYEQLIH GD  F  +EVDD
Sbjct: 121 YIQEAKDALRLLVAES-FTIPLVVVIDDIDTPTGARLGNLEYEQLIHDGDANFTPVEVDD 180

Query: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240
           EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF
Sbjct: 181 EWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTF 240

Query: 241 TWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIGSIVN 300
           TWGIAARGGTN+CMRNTTA DIFRNI+LH VTHMCCAPIVFRIIL+ADNG  +R+   VN
Sbjct: 241 TWGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFRIILEADNGDRRRVAWPVN 300

Query: 301 VMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKLKARQ 360
           V+TGGAPPPA LLEKMEALGF+ITHAYGLTEATGP LVCEWQEKWNVLPAD+QA LKARQ
Sbjct: 301 VLTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQ 360

Query: 361 GISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLT 420
           GISILTLADVDVKNL TMESVPHDGRT+GEIVLRGSS+MKGYFKDP ATAAAF+NGWFLT
Sbjct: 361 GISILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPEATAAAFKNGWFLT 420

Query: 421 GDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGES 480
           GD+GVI+PDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGES
Sbjct: 421 GDIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGES 480

Query: 481 PCAFVAVHGG-----VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQ 540
           PCAFV +HGG     VSEAE+IAFCRKNLSHYMVPKKVVF+AELPK + GK+QKF LREQ
Sbjct: 481 PCAFVVLHGGGGSGRVSEAEMIAFCRKNLSHYMVPKKVVFMAELPK-ANGKVQKFMLREQ 540

Query: 541 AKLFNVSEKEVAG--GNKSDQTDGGRQSGY-DEHVLTRSRL 574
           AK   VSEK      GNKS    G R+S Y D+HV+  SRL
Sbjct: 541 AKYLEVSEKRRVSNLGNKS----GNRRSDYGDQHVIAMSRL 575

BLAST of Lag0029380.1 vs. ExPASy TrEMBL
Match: A0A0A0KT40 (Acyl:coa ligase acetate-coa synthetase-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G319910 PE=4 SV=1)

HSP 1 Score: 973.8 bits (2516), Expect = 3.2e-280
Identity = 481/577 (83.36%), Postives = 522/577 (90.47%), Query Frame = 0

Query: 4   LSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLNVTK 63
           L KCDANY+ LTPITFLKRASA Y+DRTSIIYEGTRFTW+QTYERC RLASSLCRL V+K
Sbjct: 2   LLKCDANYSALTPITFLKRASAFYADRTSIIYEGTRFTWKQTYERCCRLASSLCRLTVSK 61

Query: 64  NDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYEYIQ 123
           NDVVSV+APN+PALYEMHFAVPMAGAILNTINTRLDIKNIA+ILRHSEAK+FFVDY+YIQ
Sbjct: 62  NDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDIKNIALILRHSEAKIFFVDYQYIQ 121

Query: 124 QAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFVSMEVDDEWD 183
           +AK+AL +L AES S +PLVVVIDDID+PTGARLG+LEYEQLI+ GD  F  +EVDDEWD
Sbjct: 122 EAKDALRLLVAES-SPIPLVVVIDDIDTPTGARLGNLEYEQLIYDGDANFTPVEVDDEWD 181

Query: 184 SIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWG 243
           SIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWG
Sbjct: 182 SIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWG 241

Query: 244 IAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIGSIVNVMT 303
           IAARGGTN+CMRNTTA DIFRNI+LH VTHMCCAPIVF IIL+ADN   +R+   VNV+T
Sbjct: 242 IAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFTIILEADNRDRRRVAWPVNVLT 301

Query: 304 GGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKLKARQGIS 363
           GGAPPPAPLLEKMEALGF+ITHAYGLTEATGP LVCEWQEKWNVLPAD+QA LKARQGIS
Sbjct: 302 GGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQGIS 361

Query: 364 ILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGWFLTGDV 423
           ILTLADVDVKNL TMESVPHDGRT+GEIVLRGSS+MKGYFKDPAATAAA +NGWFLTGD+
Sbjct: 362 ILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPAATAAALKNGWFLTGDI 421

Query: 424 GVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESPCA 483
           GVI+PDGY+EIKDRSKDVIISGGENISSVE+ETVLYRHPQV+EAAVVAMPHPRWGESPCA
Sbjct: 422 GVIYPDGYVEIKDRSKDVIISGGENISSVEVETVLYRHPQVSEAAVVAMPHPRWGESPCA 481

Query: 484 FVAVHGG-----VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKL 543
           FV +  G     VSEAE++AFCRKNLSHYMVPKKVVFVAELPK + GK+QKF LREQAK 
Sbjct: 482 FVVLQDGGGSGRVSEAEMVAFCRKNLSHYMVPKKVVFVAELPK-ANGKVQKFMLREQAKY 541

Query: 544 FNVSEKEVAG--GNKSDQTDGGRQSGYDEHVLTRSRL 574
             VSEK +    GNKS    G R+S Y EHV+  SRL
Sbjct: 542 LEVSEKRIVSNMGNKS----GNRRSDYGEHVIAMSRL 572

BLAST of Lag0029380.1 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 694.9 bits (1792), Expect = 5.5e-200
Identity = 340/563 (60.39%), Postives = 421/563 (74.78%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           MD L  C+AN  PLTPITFLKRAS  Y +RTSIIY  TRFTW QTY+RC RLA+SL  LN
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           +T+NDVVS++APN+PA+YEMHF+VPM GA+LN INTRLD K IAIILRH+E K+ FVDYE
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 YIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFVS----M 180
           +    +E L ++        P +++I++IDS T      L+YE LI +G+    S     
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASMF 180

Query: 181 EVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            V +E D I+LNYTSGTT+ PKGVV SH+GA+LS LS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIG 300
           GWT TW +AARGGTNVC+R+ TA +I++NI+LH VTHM C P VFR +L+          
Sbjct: 241 GWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKS 300

Query: 301 SIVNVMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKL 360
           S V V+TGG+ PPA L++K+E LGF++ H YGLTEATGP L CEWQ++WN LP  +Q +L
Sbjct: 301 SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIEL 360

Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNG 420
           + RQG+  LTLADVDVKN  T+ESVP DG+T GEIV++GSS+MKGY K+P AT+ AF++G
Sbjct: 361 QQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  TGD+GVIHPDGY+EIKDRSKD+IISGGENISS+E+E VLY + +V EAAVVAMPHP 
Sbjct: 421 WLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMPHPL 480

Query: 481 WGESPCAFVAVHGG-----VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFK 540
           WGE+PCAFV +  G      SE ++I +CR+N+ H+M PKKVVF  ELPK S GKI K K
Sbjct: 481 WGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILKSK 540

Query: 541 LREQAKLFNVSEKEVAGGNKSDQ 555
           LR+ AK   V E + AG  K  Q
Sbjct: 541 LRDIAKALVVREDD-AGSKKVHQ 562

BLAST of Lag0029380.1 vs. TAIR 10
Match: AT1G65880.1 (benzoyloxyglucosinolate 1 )

HSP 1 Score: 688.7 bits (1776), Expect = 3.9e-198
Identity = 341/569 (59.93%), Postives = 423/569 (74.34%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           MD L+ C+AN  PLTP+TFLKRAS  Y +RTSIIY  TRFTW QTY+RC RLA+SL  LN
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           ++KNDVVSV+APN PALYEMHFAVPMAGA+LN INTRLD  +IA ILRH++ K+ F+D  
Sbjct: 61  ISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRS 120

Query: 121 YIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGD----VGFVSM 180
           +   A+E+LH+L++E  +    V+ I + D P  A    L+YE LI RG+    +     
Sbjct: 121 FEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLIQRGEPTPSMVARMF 180

Query: 181 EVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            + DE D I+LNYTSGTT+ PKGVV SHRGA+L TLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIG 300
           GWTFTWG AARGGT+VCMR+ TA +I++NI++H+VTHMCC P VF I+L  ++       
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRS 300

Query: 301 SIVNVMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKL 360
             V+V+TGG+PPPA L++K++ LGF + HAYG TEATGP L CEWQ++WN LP ++Q +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNG 420
           KARQGISIL LADVDVKN +T +S P DG+T GEI+++GSSIMKGY K+P AT  AF++G
Sbjct: 361 KARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKATFEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  TGDVGVIHPDG++EIKDRSKD+IISGGENISSVE+E VLY++P+V E AVVAMPHP 
Sbjct: 421 WLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFVAVHGG-------------VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTS 540
           WGE+PCAFV +                  E  +I +CR+NL H+M P+KVVF+ ELPK  
Sbjct: 481 WGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKNG 540

Query: 541 TGKIQKFKLREQAK------LFNVSEKEV 547
            GKI K KLR+ AK        NV  KEV
Sbjct: 541 NGKILKPKLRDIAKGLVVEDEINVIAKEV 569

BLAST of Lag0029380.1 vs. TAIR 10
Match: AT1G65890.1 (acyl activating enzyme 12 )

HSP 1 Score: 686.4 bits (1770), Expect = 1.9e-197
Identity = 336/562 (59.79%), Postives = 423/562 (75.27%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           MD L+ C+AN  PLTPITFLKRAS  Y +RTSIIY  TRFTW QTY+RC RLA+SL  LN
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           + KNDVVSV+APN PA+YEMHFAVPMAGA+LN INTRLD  +IA ILRH++ K+ F+   
Sbjct: 61  IGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRS 120

Query: 121 YIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGSLEYEQLIHRGDVGFVSME--- 180
           +   A+E L +L++E  +    V+ I +ID P        +YE LI RG+   + +    
Sbjct: 121 FEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQRGEPTPLLLARMF 180

Query: 181 -VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            + DE D I+LNYTSGTT+ PKGVV SHRGA+LSTLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 CIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIG 300
           GWTFTWG AARGGT+VCMR+ TA +I++NI++H+VTHMCC P VF I+L  ++       
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSHRS 300

Query: 301 SIVNVMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKL 360
             V+V+TGG+PPPA L++K++ LGF + HAYGLTEATGP L CEWQ++WN LP ++Q +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNG 420
           KARQG+SIL L +VDV+N +T ESVP DG+T GEIV++GSSIMKGY K+P AT  AF++G
Sbjct: 361 KARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  +GDVGVIHPDG++EIKDRSKD+IISGGENISSVE+E ++Y++P+V E AVVAMPHP 
Sbjct: 421 WLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFVAVHGG------------VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTST 540
           WGE+PCAFV +  G              E ++I +CR+NL H+M P+KVVF+ ELPK   
Sbjct: 481 WGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPKNGN 540

Query: 541 GKIQKFKLREQAKLFNVSEKEV 547
           GKI K KLR+ AK   V+E EV
Sbjct: 541 GKILKPKLRDIAKGL-VAEDEV 561

BLAST of Lag0029380.1 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 585.5 bits (1508), Expect = 4.7e-167
Identity = 283/550 (51.45%), Postives = 390/550 (70.91%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIYEGTRFTWRQTYERCRRLASSLCRLN 60
           M+   K  ANY PLTPI+FL R++  Y+DR SI+Y   ++TWRQT +RC R+AS+L +L 
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  VTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDYE 120
           ++  DVVSV+APN+PA+ E+HF VPMAGA+L T+N R D   +A++LRHS  K+ F D++
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 YIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLGS---LEYEQLIHRGDVGFVSME 180
           ++Q A+ A  +L+ +      LV++ + +      +  S   +EYE ++  G   F  + 
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 181 VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNG 240
             DE D+I++NYTSGTTS+PKGVVYSHRGA+L++L+ V+  EM ++P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 241 WTFTWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKRIGS 300
           W   WG+ A GGTN+C+RN TA  IF NI  H VTHM  AP +  +I++A     K +  
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 301 IVNVMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQAKLK 360
            V+ +TG APPPA ++ KME LGF + H+YGLTE  GPG +C W+ +W+ LP +EQAK+K
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

Query: 361 ARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFRNGW 420
           ARQG++ L L ++ VK+  TM ++P DG T GE+V RG+++M GY K+P AT  AF+ GW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422

Query: 421 FLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRW 480
           F +GD+GV HPDGY+E+KDRSKD+IISGGENISS+E+E+ L+ HP V EAAVVA P   W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482

Query: 481 GESPCAFVAVHGG--VSEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQ 540
           GE+ CAFV +  G   S  E+I++CR  L HYM P+ +VF  +LPKTSTGK+QKF LR +
Sbjct: 483 GETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVF-EDLPKTSTGKVQKFVLRTK 542

Query: 541 AK-LFNVSEK 545
           AK L ++S+K
Sbjct: 543 AKALVSLSKK 551

BLAST of Lag0029380.1 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 579.7 bits (1493), Expect = 2.6e-165
Identity = 289/547 (52.83%), Postives = 380/547 (69.47%), Query Frame = 0

Query: 1   MDKLSKCDANYAPLTPITFLKRASASYSDRTSIIY-EGTRFTWRQTYERCRRLASSLCRL 60
           M+++  C AN  PLTPI FL+RA+  Y D TSI+Y   T +TWR+T  RC R+ASSL  +
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  NVTKNDVVSVIAPNIPALYEMHFAVPMAGAILNTINTRLDIKNIAIILRHSEAKLFFVDY 120
            + ++DVVSV++PN PA+YE+ FAVPM+GAILN INTRLD + ++++LRH  +KL FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 EYIQQAKEALHVLAAESPSTLPLVVVIDDIDSPTGARLG-----SLEYEQLIHRGDVGFV 180
             +  A EA+ ++  + P    LV + D  +    A +      S  Y+ LIHRGD+ F 
Sbjct: 121 FSVDLAVEAISMMTTDPPI---LVFIADKEEEGGDADVADRTKFSYTYDDLIHRGDLDFK 180

Query: 181 SMEVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFH 240
            +  + EWD + LNYTSGTTSAPKGVV+ HRG F+ ++  ++ W +   PVYLWTLP+FH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFH 240

Query: 241 CNGWTFTWGIAARGGTNVCMRNTTAFDIFRNIDLHHVTHMCCAPIVFRIILDADNGSPKR 300
            NGW++ WGIAA GGTNVC+R   A  I+R I  H VTHMC AP+V  ++   +   P  
Sbjct: 241 ANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQP-- 300

Query: 301 IGSIVNVMTGGAPPPAPLLEKMEALGFYITHAYGLTEATGPGLVCEWQEKWNVLPADEQA 360
           +   VN++T GAPPPA +L + E++GF I+H YGLTE  G  + C W+ +WN LPA ++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 KLKARQGISILTLADVDVKNLDTMESVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAAFR 420
           +LKARQG+  +   ++DV + ++  SV  +G T GEIV+RGSSIM GY KDP  T  A +
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKALK 420

Query: 421 NGWFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPH 480
           NGWF TGDVGVIH DGYLEIKDRSKD+II+GGEN+SSVE+ETVLY +P V E AVVA P 
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPD 480

Query: 481 PRWGESPCAFVAVHGGV----SEAEIIAFCRKNLSHYMVPKKVVFVAELPKTSTGKIQKF 538
             WGE+PCAFV++  G+    +E E+I +CRK +  YMVPK V FV ELPKTSTGK+ KF
Sbjct: 481 VFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKF 540