Lag0029255.1 (mRNA) Sponge gourd (AG‐4) v1

BLAST of Lag0029255.1 vs. NCBI nr
Match: XP_038884439.1 (protein NODULATION SIGNALING PATHWAY 2 [Benincasa hispida])

HSP 1 Score: 767.3 bits (1980), Expect = 8.6e-218
Identity = 406/533 (76.17%), Postives = 433/533 (81.24%), Query Frame = 0

Query: 2   IQTVNSPMALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVDWEPFPGTHVDF 61
           I ++NSPMALA+DN F ++ LS+YSTSTNNSDD  HLAGNWNY SP+VDWE FP TH DF
Sbjct: 16  INSLNSPMALAMDNLFNEITLSNYSTSTNNSDDHRHLAGNWNYGSPIVDWETFPDTHNDF 75

Query: 62  HDLFDSMMAVDAPPYPPRAAEVDSGSNCNSGSSPAEDQEEEEEEAKAEDREEEEYKGLRL 121
           HDL DSM+ +D PP                  SP ED+E           EEE++KGLRL
Sbjct: 76  HDLVDSMICIDNPP------------------SPTEDRE-----------EEEDFKGLRL 135

Query: 122 YHLLMAAADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAV 181
           YHLL+AAADA+FGDHKSRDLAHVILVRLNELVSPSHGTNL+RLTAYYAQAFQ LLD   V
Sbjct: 136 YHLLVAAADAVFGDHKSRDLAHVILVRLNELVSPSHGTNLQRLTAYYAQAFQDLLDSTPV 195

Query: 182 SGGAGGGSNKPHHHHHHSHHHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEA 241
                  SNK HHH    +HHLHRDDH+PTDVLAAFQLLQEMSPYVKF HFTANQAILEA
Sbjct: 196 -------SNKTHHH----NHHLHRDDHTPTDVLAAFQLLQEMSPYVKFSHFTANQAILEA 255

Query: 242 VADDRRVHIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATCRRSIATV 301
           VA+DRRVHIVDYDIMEG QWASLMQAFVS    P APHLRITAISRG  G   RRSI TV
Sbjct: 256 VAEDRRVHIVDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISRGANG---RRSIGTV 315

Query: 302 QETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFIYRTP 361
           QETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLV+GEALVVNC+LHLPHF YR+P
Sbjct: 316 QETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCILHLPHFSYRSP 375

Query: 362 ESIASFLSGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKK 421
           ESI SFLSG K+LNPR+VTLVEEEIGHGPT+D DYKVQFLDSLERYSAIYDSL A  P K
Sbjct: 376 ESIGSFLSGTKSLNPRIVTLVEEEIGHGPTIDVDYKVQFLDSLERYSAIYDSLEAVIPMK 435

Query: 422 NRARALVERVFLGPRISATLTRIGQ-------PQANCSWGERLEKLGFKAAAISFANHCQ 481
           NRARALVERVFLGPRISATL RIGQ        + NC WGE+LEK+G K A ISFANHCQ
Sbjct: 436 NRARALVERVFLGPRISATLRRIGQRRWATAGAEENCLWGEKLEKMGLKPATISFANHCQ 495

Query: 482 ARLLLDLFNDGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSCSSSLCSSSDSE 528
           ARLLL LFNDGYRVEELGNNKLVLGWKSKRLLS SIWVSSS SSS  S SDSE
Sbjct: 496 ARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSASIWVSSSLSSSSSSLSDSE 502

BLAST of Lag0029255.1 vs. NCBI nr
Match: XP_023551167.1 (nodulation-signaling pathway 2 protein isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 758.1 bits (1956), Expect = 5.2e-215
Identity = 406/526 (77.19%), Postives = 431/526 (81.94%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVDWEPFPGTHVDFHDLFDSM 68
           MA+A+DN F  +A+SDYSTSTNNSDD                         DFHDLFDS+
Sbjct: 1   MAMAVDNAFNGVAVSDYSTSTNNSDD-------------------------DFHDLFDSI 60

Query: 69  MAVDAPPYPPRAAEVDSGSNCNSGSSPAEDQEEEEEEAKAEDREEEEYKGLRLYHLLMAA 128
           M++DA PY P+  EVD G+NCNS S+PAED+EE          EEEE+K LRLYHLLMA 
Sbjct: 61  MSIDALPYSPQVVEVDGGTNCNSPSNPAEDREE----------EEEEFKSLRLYHLLMAV 120

Query: 129 ADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSGGAGGG 188
           ADA+FGDHKSRDLA VIL+RLNELVS SHGTNLERLTAYYAQ+FQ LLD AAVS   GGG
Sbjct: 121 ADAMFGDHKSRDLAQVILIRLNELVSTSHGTNLERLTAYYAQSFQDLLDYAAVS---GGG 180

Query: 189 SNKPHHHHHHSHHHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRV 248
           SNKP        HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRV
Sbjct: 181 SNKP--------HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRV 240

Query: 249 HIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATCRRSIATVQETGRRL 308
           HIVDY+IMEG QWASLMQA VSRKD PPAPHLRITAISR   G + RR I TVQETGRRL
Sbjct: 241 HIVDYNIMEGIQWASLMQALVSRKDSPPAPHLRITAISR---GPSSRRWIGTVQETGRRL 300

Query: 309 VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFIYRTPESIASFL 368
           VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNC+LHLPHF   +PESI SFL
Sbjct: 301 VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIDSFL 360

Query: 369 SGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALV 428
           +GAKTLNPRLVTLVEEEI HGPT+DGDYKVQFLDSLERYSAIYDSL AG P KNRAR LV
Sbjct: 361 AGAKTLNPRLVTLVEEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLV 420

Query: 429 ERVFLGPRISATLTRIGQPQ-------ANCSWGERLEKLGFKAAAISFANHCQARLLLDL 488
           E+VFLGPRISATL RIGQPQ        NCSWGERLEK+G KAAAISFANHCQARLL+DL
Sbjct: 421 EKVFLGPRISATLKRIGQPQRAATAAEENCSWGERLEKMGLKAAAISFANHCQARLLVDL 476

Query: 489 FNDGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSCSSSLCSSSDSE 528
           FNDGYRVEELG+NKLVLGWKSKRLLS SIW SSS SSS  SSSDSE
Sbjct: 481 FNDGYRVEELGSNKLVLGWKSKRLLSVSIWASSSLSSS-SSSSDSE 476

BLAST of Lag0029255.1 vs. NCBI nr
Match: XP_008445682.1 (PREDICTED: nodulation-signaling pathway 2 protein [Cucumis melo] >KAA0063953.1 nodulation-signaling pathway 2 protein [Cucumis melo var. makuwa] >TYK05186.1 nodulation-signaling pathway 2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 756.5 bits (1952), Expect = 1.5e-214
Identity = 401/527 (76.09%), Postives = 428/527 (81.21%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVDWEPFPGTHVDFHDLFDSM 68
           MALAI+NPF D+ALS+YSTSTNNSDD  HLAGNWNY SP+VDWE F GTH DF D+FDS 
Sbjct: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60

Query: 69  MAVDAPPYPPRAAEVDSGSNCNSGSSPAEDQEEEEEEAKAEDR-EEEEYKGLRLYHLLMA 128
           + ++ PP+                                EDR EE+E+KGLRLYHLL+A
Sbjct: 61  ICIENPPF------------------------------LTEDRDEEDEFKGLRLYHLLIA 120

Query: 129 AADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSGGAGG 188
           AADA+FGDH+S DLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQ LLD A V      
Sbjct: 121 AADAVFGDHRSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLDSAPV------ 180

Query: 189 GSNKPHHHHHHSHHHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRR 248
            SNK HHH+HH H   HRDDH+PTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVA+DRR
Sbjct: 181 -SNKTHHHNHHIH---HRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRR 240

Query: 249 VHIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATCRRSIATVQETGRR 308
           VHIVDYDIMEG QWASLMQAFVS    P APHLRITAISRG  G   RRSI TVQETGRR
Sbjct: 241 VHIVDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISRGANG---RRSIGTVQETGRR 300

Query: 309 LVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFIYRTPESIASF 368
           LVAFAASIGQPFSFHQCKLDSDESFRPSGLKLV+GEALVVNCMLHLPHF YR+PESIASF
Sbjct: 301 LVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASF 360

Query: 369 LSGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARAL 428
           LSGAK+L+PR+VTLVEEEIGHGPT+DGDYKVQFLDSLERYSAIYDSL A  P KNRARAL
Sbjct: 361 LSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARAL 420

Query: 429 VERVFLGPRISATLTRIGQ-------PQANCSWGERLEKLGFKAAAISFANHCQARLLLD 488
           VERVFLGPRISATL RIGQ        + NC WGE+LEK+G K   ISFANHCQARLLL 
Sbjct: 421 VERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLG 480

Query: 489 LFNDGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSCSSSLCSSSDSE 528
           LFNDGYRVEELGNNKLVLGWKSKRLLS SIW SS+ SSS  S SDSE
Sbjct: 481 LFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDSE 481

BLAST of Lag0029255.1 vs. NCBI nr
Match: KAG6578977.1 (Protein NODULATION SIGNALING PATHWAY 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 755.7 bits (1950), Expect = 2.6e-214
Identity = 403/526 (76.62%), Postives = 430/526 (81.75%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVDWEPFPGTHVDFHDLFDSM 68
           MA+A+DN F  +A+SDYSTSTNNSDD                         DFHDLFDS+
Sbjct: 1   MAMAVDNAFNGVAVSDYSTSTNNSDD-------------------------DFHDLFDSI 60

Query: 69  MAVDAPPYPPRAAEVDSGSNCNSGSSPAEDQEEEEEEAKAEDREEEEYKGLRLYHLLMAA 128
           M++DA PY P+  E+D G+NCNS S+PAE +EE          EEEE++ LRLYHLLMA 
Sbjct: 61  MSIDALPYSPQVVEIDGGTNCNSPSNPAEGREE----------EEEEFRSLRLYHLLMAV 120

Query: 129 ADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSGGAGGG 188
           ADA+FGDHKSR+LA VIL+RLNELVS SHGTNLERLTAYYAQAFQ LLDCAAVS   GGG
Sbjct: 121 ADAMFGDHKSRELAQVILIRLNELVSTSHGTNLERLTAYYAQAFQDLLDCAAVS---GGG 180

Query: 189 SNKPHHHHHHSHHHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRV 248
            NKP        HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRV
Sbjct: 181 PNKP--------HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRV 240

Query: 249 HIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATCRRSIATVQETGRRL 308
           HIVDY+IMEG QWASLMQA VSRKD PPAPHLRITAISR   G + RR I TVQETGRRL
Sbjct: 241 HIVDYNIMEGIQWASLMQALVSRKDSPPAPHLRITAISR---GPSSRRWIGTVQETGRRL 300

Query: 309 VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFIYRTPESIASFL 368
           VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNC+LHLPHF   +PESIASFL
Sbjct: 301 VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIASFL 360

Query: 369 SGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALV 428
           +GAKTLNPRLVTLVEEEI HGPT+DGDYKVQFLDSLERYSAIYDSL AG P KNRAR LV
Sbjct: 361 AGAKTLNPRLVTLVEEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLV 420

Query: 429 ERVFLGPRISATLTRIGQPQ-------ANCSWGERLEKLGFKAAAISFANHCQARLLLDL 488
           E+VFLGPRISATL RIGQPQ        NC WGERLEK+G KAAAISFANHCQARLL+DL
Sbjct: 421 EKVFLGPRISATLRRIGQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDL 476

Query: 489 FNDGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSCSSSLCSSSDSE 528
           FNDGYRVEELG+NKLVLGWKSKRLLS SIW SSS SSS  SSSDSE
Sbjct: 481 FNDGYRVEELGSNKLVLGWKSKRLLSVSIWASSSLSSS-SSSSDSE 476

BLAST of Lag0029255.1 vs. NCBI nr
Match: KAG7016501.1 (Nodulation-signaling pathway 2 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 755.0 bits (1948), Expect = 4.4e-214
Identity = 402/526 (76.43%), Postives = 430/526 (81.75%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVDWEPFPGTHVDFHDLFDSM 68
           MA+A+DN F  +A+SDYSTSTNNSDD                         DFHDLFDS+
Sbjct: 1   MAMAVDNAFNGVAVSDYSTSTNNSDD-------------------------DFHDLFDSI 60

Query: 69  MAVDAPPYPPRAAEVDSGSNCNSGSSPAEDQEEEEEEAKAEDREEEEYKGLRLYHLLMAA 128
           M++DA PY P+  E+D G+NCNS S+PAE +EE          EEEE++ LRLYHLLMA 
Sbjct: 61  MSIDALPYSPQVVEIDGGTNCNSPSNPAEGREE----------EEEEFRSLRLYHLLMAV 120

Query: 129 ADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSGGAGGG 188
           ADA+FGDHKSR+LA VIL+RLNELVS SHGTNLER+TAYYAQAFQ LLDCAAVS   GGG
Sbjct: 121 ADAMFGDHKSRELAQVILIRLNELVSTSHGTNLERITAYYAQAFQDLLDCAAVS---GGG 180

Query: 189 SNKPHHHHHHSHHHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRV 248
            NKP        HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRV
Sbjct: 181 PNKP--------HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRV 240

Query: 249 HIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATCRRSIATVQETGRRL 308
           HIVDY+IMEG QWASLMQA VSRKD PPAPHLRITAISR   G + RR I TVQETGRRL
Sbjct: 241 HIVDYNIMEGIQWASLMQALVSRKDSPPAPHLRITAISR---GPSSRRWIGTVQETGRRL 300

Query: 309 VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFIYRTPESIASFL 368
           VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNC+LHLPHF   +PESIASFL
Sbjct: 301 VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIASFL 360

Query: 369 SGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALV 428
           +GAKTLNPRLVTLVEEEI HGPT+DGDYKVQFLDSLERYSAIYDSL AG P KNRAR LV
Sbjct: 361 AGAKTLNPRLVTLVEEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLV 420

Query: 429 ERVFLGPRISATLTRIGQPQ-------ANCSWGERLEKLGFKAAAISFANHCQARLLLDL 488
           E+VFLGPRISATL RIGQPQ        NC WGERLEK+G KAAAISFANHCQARLL+DL
Sbjct: 421 EKVFLGPRISATLRRIGQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDL 476

Query: 489 FNDGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSCSSSLCSSSDSE 528
           FNDGYRVEELG+NKLVLGWKSKRLLS SIW SSS SSS  SSSDSE
Sbjct: 481 FNDGYRVEELGSNKLVLGWKSKRLLSVSIWASSSLSSS-SSSSDSE 476

BLAST of Lag0029255.1 vs. ExPASy Swiss-Prot
Match: Q5NE24 (Protein NODULATION SIGNALING PATHWAY 2 OS=Medicago truncatula OX=3880 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.4e-146
Identity = 291/524 (55.53%), Postives = 365/524 (69.66%), Query Frame = 0

Query: 19  DLALSDYSTSTNNSDDAHHLAGNWNYLSPVVDWEPFPGTHVDFHDLFDSMMAVDAPPYPP 78
           DL  S +S+ TN           WN+ SP+V+W+ F G   DFH L D+++         
Sbjct: 11  DLHFSGHSSLTNTPTSDEDYGCTWNHWSPIVNWDTFTGAPDDFHHLMDTIIEDRTTVLEQ 70

Query: 79  RAAEVDSGSNCNSGSSPAEDQEEEEEE--------AKAEDREEEEYKGLRLYHLLMAAAD 138
            +  + +     + ++   D+EEEE E        A       ++ KGL+L HLLMA A+
Sbjct: 71  LSPSITT----TTTTTTTTDEEEEEMETTTTTTTTAIKTHEVGDDSKGLKLVHLLMAGAE 130

Query: 139 ALFGDHKSRDLAHVILVRLNELVSP-SHGTNLERLTAYYAQAFQGLLDCAAVSGGAGGGS 198
           AL G  K+RDLA VIL+RL ELVS  ++G+N+ERL A++ +A  GLL+      GAGG  
Sbjct: 131 ALTGSTKNRDLARVILIRLKELVSQHANGSNMERLAAHFTEALHGLLE------GAGGAH 190

Query: 199 NKPHHHHHHSHHHLHRDDH-SPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRV 258
           N  HHH+++ H+      H +  D LAAFQLLQ+MSPYVKFGHFTANQAI+EAVA +RRV
Sbjct: 191 NNHHHHNNNKHYLTTNGPHDNQNDTLAAFQLLQDMSPYVKFGHFTANQAIIEAVAHERRV 250

Query: 259 HIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATCRRSIATVQETGRRL 318
           H++DYDIMEG QWASL+Q+  S  +G   PHLRITA+SR G G   RRSIATVQETGRRL
Sbjct: 251 HVIDYDIMEGVQWASLIQSLASNNNG---PHLRITALSRTGTG---RRSIATVQETGRRL 310

Query: 319 VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFIYRTPESIASFL 378
            +FAAS+GQPFSFH C+LDSDE+FRPS LKLVRGEALV NCML+LPH  YR PES+ASFL
Sbjct: 311 TSFAASLGQPFSFHHCRLDSDETFRPSALKLVRGEALVFNCMLNLPHLSYRAPESVASFL 370

Query: 379 SGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALV 438
           +GAKTLNP+LVTLVEEE+G   ++ G +  +F+DSL  YSA++DSL AGFP +NRAR LV
Sbjct: 371 NGAKTLNPKLVTLVEEEVG---SVIGGFVERFMDSLHHYSAVFDSLEAGFPMQNRARTLV 430

Query: 439 ERVFLGPRISATLTRI---GQPQANCSWGERLEKLGFKAAAISFANHCQARLLLDLFNDG 498
           ERVF GPRI+ +L RI   G  +   SWGE L ++GF+   +SFANHCQA+LLL LFNDG
Sbjct: 431 ERVFFGPRIAGSLGRIYRTGGEEERRSWGEWLGEVGFRGVPVSFANHCQAKLLLGLFNDG 490

Query: 499 YRVEE--LGNNKLVLGWKSKRLLSTSIWVSSSCSSSLCSSSDSE 528
           YRVEE  +G+NKLVL WKS+RLLS S+W         CSSSDS+
Sbjct: 491 YRVEEVGVGSNKLVLDWKSRRLLSASLWT--------CSSSDSD 507

BLAST of Lag0029255.1 vs. ExPASy Swiss-Prot
Match: Q2PEG7 (Protein NODULATION SIGNALING PATHWAY 2 OS=Lotus japonicus OX=34305 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 3.8e-144
Identity = 292/515 (56.70%), Postives = 353/515 (68.54%), Query Frame = 0

Query: 20  LALSDYSTSTNN-SDDAHHLAGNWNYLSPVVDWEPFPGTHVDFHDLFDSMMAVDAPPYPP 79
           L  S +ST TN  S D  +   +WN+ SPVV+W+ F G   DFH L DSM+         
Sbjct: 11  LDFSGHSTLTNTPSSDNDNYGCSWNHWSPVVNWDAFTGNQDDFHHLIDSMI--------- 70

Query: 80  RAAEVDSGSNCNSGSSPAEDQEEEEEEA-------------KAEDREEEEYKGLRLYHLL 139
              + ++G   +  ++    +EEEEEEA                +  ++++KGLRL HLL
Sbjct: 71  --DDNNTGPAFSDHTASTTSEEEEEEEATTTTMTTTTTTTTTTPEAADDDFKGLRLVHLL 130

Query: 140 MAAADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSGGA 199
           MA A+AL G +K+R+LA VILVRL ELVS + GTN+ERL AY+ +A QGLL+      GA
Sbjct: 131 MAGAEALTGANKNRELARVILVRLKELVSHTDGTNMERLAAYFTEALQGLLE------GA 190

Query: 200 GGGSNKPHHHHHHSHHHLHRDDHSP-TDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAD 259
           GG  N    HH     H     H P  D LAAFQLLQ+MSPYVKFGHFTANQAI+EAVA 
Sbjct: 191 GGAYNSSSKHHVIGGPH-----HEPQNDALAAFQLLQDMSPYVKFGHFTANQAIVEAVAH 250

Query: 260 DRRVHIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATCRRSIATVQET 319
           +RRVHIVDYDIMEG QWASLMQA  S  +G   PHLRITA+SR G G   RRS+ATVQET
Sbjct: 251 ERRVHIVDYDIMEGVQWASLMQALASNPNG---PHLRITALSRSGVG---RRSMATVQET 310

Query: 320 GRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFIYRTPESI 379
           GRRL AFA S+GQPFSFH  +L+SDE+FRP+GLKLVRGEALV NCML+LPH  YR+P S+
Sbjct: 311 GRRLTAFATSLGQPFSFHHSRLESDETFRPAGLKLVRGEALVFNCMLNLPHLTYRSPNSV 370

Query: 380 ASFLSGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRA 439
           ASFL+ AK L PRLVT+VEEE+G   +  G +  +F+DSL  +SA++DSL AGFP + RA
Sbjct: 371 ASFLTAAKALRPRLVTVVEEEVG---SALGGFVERFMDSLHHFSAVFDSLEAGFPMQGRA 430

Query: 440 RALVERVFLGPRISATLTRI----GQPQANCSWGERLEKLGFKAAAISFANHCQARLLLD 499
           RALVERVFLGPRI  +L RI    G  +   SW E L   GF   A+S ANHCQ+ LLL 
Sbjct: 431 RALVERVFLGPRIVGSLARIYRTGGGGEERGSWREWLRAAGFSGVAVSSANHCQSNLLLG 490

Query: 500 LFNDGYRVEELGNNKLVLGWKSKRLLSTSIWVSSS 516
           LFNDGYRVEELG+NKLVL WK++RLLS S+W  SS
Sbjct: 491 LFNDGYRVEELGSNKLVLHWKTRRLLSASLWTCSS 494

BLAST of Lag0029255.1 vs. ExPASy Swiss-Prot
Match: Q84Q92 (Protein NODULATION SIGNALING PATHWAY 2 OS=Oryza sativa subsp. japonica OX=39947 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 2.5e-119
Identity = 262/542 (48.34%), Postives = 323/542 (59.59%), Query Frame = 0

Query: 11  LAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVDWEPF-----PGTHVDFHDLF 70
           +A D  F+    +  ++S ++ DD   +   W  LSPV DW  F      G H D H L 
Sbjct: 8   VAGDFEFSGCGSTTTTSSASSLDDGTGMCYAWGELSPVADWANFCCSDDDGGH-DLHGLI 67

Query: 71  DSMMAVDAPPYPPRAAEVDSGSNC---------------NSGSSPAEDQEEEEEEAKAED 130
           +SM+  D          VD                     +GS+P+            +D
Sbjct: 68  ESMLCDDT------LVGVDDDGQAGLHHADMFRDDLYCYGNGSNPSSTTTTNPGSPVFDD 127

Query: 131 REEE-EYKGLRLYHLLMAAADALFGDHKSRDLAHVILVRLNELVS-----PSHGTNLERL 190
             +    KGLRL HLLMAAA+AL G HKSR+LA VILVRL E+VS      +  +N+ERL
Sbjct: 128 PTQGCPEKGLRLLHLLMAAAEALSGPHKSRELARVILVRLKEMVSHTASANAAASNMERL 187

Query: 191 TAYYAQAFQGLLDCAAVSGGAGGGSNKPHHHHHHSHHHLHRDDHSPTDVLAAFQLLQEMS 250
            A++  A QGLLD +   GG+G  +     HHH              DVL AFQ+LQ+MS
Sbjct: 188 AAHFTDALQGLLDGSHPVGGSGRQAAAAASHHHAG------------DVLTAFQMLQDMS 247

Query: 251 PYVKFGHFTANQAILEAVADDRRVHIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITA 310
           PY+KFGHFTANQAILEAV+ DRRVHIVDYDI EG QWASLMQA  SR DG PAPHLRITA
Sbjct: 248 PYMKFGHFTANQAILEAVSGDRRVHIVDYDIAEGIQWASLMQAMTSRADGVPAPHLRITA 307

Query: 311 ISRGGGGATCRRSIATVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEA 370
           +SR GGG         VQE GRRL AFAASIGQPFSF QC+LDSDE FRP+ +++V+GEA
Sbjct: 308 VSRSGGGGA-----RAVQEAGRRLSAFAASIGQPFSFGQCRLDSDERFRPATVRMVKGEA 367

Query: 371 LVVNCMLH---LPHFIYRTPESIASFLSGAKTLNPRLVTLVEEEIGHGPTMDGD------ 430
           LV NC+LH       I R   S+ASFLSG   L  +LVT+VEEE       DGD      
Sbjct: 368 LVANCVLHQAAATTTIRRPTGSVASFLSGMAALGAKLVTVVEEEGEAEKDDDGDSAGDAA 427

Query: 431 ---YKVQFLDSLERYSAIYDSLGAGFPKKNRARALVERVFLGPRISATLTRIGQ---PQA 490
              +  QF++ L RYSA++DSL AGFP ++R R LVERV L P I+  ++R  +    + 
Sbjct: 428 AGGFVRQFMEELHRYSAVWDSLEAGFPTQSRVRGLVERVILAPNIAGAVSRAYRGVDGEG 487

Query: 491 NCSWGERLEKLGFKAAAISFANHCQARLLLDLFNDGYRVEELGNNKLVLGWKSKRLLSTS 512
            C WG+ +   GF A  +S  NH QARLLL LFNDGY VEE G NK+VLGWK++RL+S S
Sbjct: 488 RCGWGQWMRGSGFTAVPLSCFNHSQARLLLGLFNDGYTVEETGPNKIVLGWKARRLMSAS 525

BLAST of Lag0029255.1 vs. ExPASy Swiss-Prot
Match: Q9SUF5 (Scarecrow-like protein 26 OS=Arabidopsis thaliana OX=3702 GN=SCL26 PE=2 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 8.8e-117
Identity = 251/508 (49.41%), Postives = 317/508 (62.40%), Query Frame = 0

Query: 16  PFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPV-VDWEPFPGTHVDFHDLFDSMMAVDAP 75
           P+ D     +ST T+    A   + N   L+ + +DW+       DF D+ +S+M  +  
Sbjct: 4   PYEDFLDLFFSTHTDPLATAASTSSNGYSLNDLDIDWD------CDFRDVIESIMGDEGA 63

Query: 76  PYPPRAAEV----DSGSNCNSGSSPAEDQEEEEEEAKAEDREEEEYKGLRLYHLLMAAAD 135
              P +  V    D    CNS S+         +     + + +E KGLRL HLL+AAAD
Sbjct: 64  MMEPESEAVPMLHDQEGLCNSASTGL----SVADGVSFGEPKTDESKGLRLVHLLVAAAD 123

Query: 136 ALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSGGAGGGSN 195
           A  G +KSR+L  VIL RL +LVSP   TN+ERL A++      LL+  +V         
Sbjct: 124 ASTGANKSRELTRVILARLKDLVSPGDRTNMERLAAHFTNGLSKLLERDSV--------- 183

Query: 196 KPHHHHHHSHHHLHRDD-HSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVH 255
                        HRDD +   DV++AF+LLQ MSPYV FG+ TA QAILEAV  +RR+H
Sbjct: 184 --------LCPQQHRDDVYDQADVISAFELLQNMSPYVNFGYLTATQAILEAVKYERRIH 243

Query: 256 IVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATCRRSIATVQETGRRLV 315
           IVDYDI EG QWASLMQA VSR  GP A HLRITA+SR   G   ++S+A VQETGRRL 
Sbjct: 244 IVDYDINEGVQWASLMQALVSRNTGPSAQHLRITALSRATNG---KKSVAAVQETGRRLT 303

Query: 316 AFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFIYRTPESIASFLS 375
           AFA SIGQPFS+  CKLD++ +F  S LKLVRGEA+V+NCMLHLP F ++TP S+ SFLS
Sbjct: 304 AFADSIGQPFSYQHCKLDTN-AFSTSSLKLVRGEAVVINCMLHLPRFSHQTPSSVISFLS 363

Query: 376 GAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVE 435
            AKTLNP+LVTLV EE+G        Y+  F+D L ++SAI+DSL AG    N AR  VE
Sbjct: 364 EAKTLNPKLVTLVHEEVGLMGNQGFLYR--FMDLLHQFSAIFDSLEAGLSIANPARGFVE 423

Query: 436 RVFLGPRISATLTRI----GQPQANCSWGERLEKLGFKAAAISFANHCQARLLLDLFNDG 495
           RVF+GP ++  LTRI     + ++  SW + LE  GFK   +SF N CQA+LLL LFNDG
Sbjct: 424 RVFIGPWVANWLTRITANDAEVESFASWPQWLETNGFKPLEVSFTNRCQAKLLLSLFNDG 478

Query: 496 YRVEELGNNKLVLGWKSKRLLSTSIWVS 514
           +RVEELG N LVLGWKS+RL+S S W S
Sbjct: 484 FRVEELGQNGLVLGWKSRRLVSASFWAS 478

BLAST of Lag0029255.1 vs. ExPASy Swiss-Prot
Match: A2ZHL0 (Protein SCARECROW 2 OS=Oryza sativa subsp. indica OX=39946 GN=SCR2 PE=3 SV=2)

HSP 1 Score: 201.4 bits (511), Expect = 2.5e-50
Identity = 149/444 (33.56%), Postives = 232/444 (52.25%), Query Frame = 0

Query: 75  PYPPRAAEVDSGSNCNSGSSPAEDQEEEEEEAKAEDREEEEYKGLRLYHLLMAAADALFG 134
           P PP A E  + +   + ++ A  +E +EE+     R++ + +GL L  LL+  A+++  
Sbjct: 250 PKPPTAEETAAAAAAAAAAAAAAAKERKEEQR----RKQRDEEGLHLLTLLLQCAESVNA 309

Query: 135 DHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSGGAGGGSNKPHH 194
           D  + D AH  L+ + EL +P  GT+ +R+ AY+A+A    L  + +   A   S  P  
Sbjct: 310 D--NLDEAHRALLEIAELATP-FGTSTQRVAAYFAEAMSARLVSSCLGLYAPLPSPSPAG 369

Query: 195 HHHHSHHHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHIVDYD 254
              H              V AAFQ+   +SP+VKF HFTANQAI EA   + RVHI+D D
Sbjct: 370 ARVHGR------------VAAAFQVFNGISPFVKFSHFTANQAIQEAFEREERVHIIDLD 429

Query: 255 IMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATCRRSIATVQETGRRLVAFAAS 314
           IM+G QW  L     SR  GP  P +R+T +           S+  ++ TG+RL  FA +
Sbjct: 430 IMQGLQWPGLFHILASRPGGP--PRVRLTGLG---------ASMEALEATGKRLSDFADT 489

Query: 315 IGQPFSFHQCKL-DSDESFRPSGLKLVRGEALVVNCMLHLPHFIYRTPESIASFLSGAKT 374
           +G PF F  C + D   +  P  L + R EA+ V+    L H +Y    S ++ L   + 
Sbjct: 490 LGLPFEF--CPVADKAGNLDPEKLGVTRREAVAVH---WLRHSLYDVTGSDSNTLWLIQR 549

Query: 375 LNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVERVFL 434
           L P++VT+VE+++ H     G +  +F++++  YSA++DSL A + + +  R +VE+  L
Sbjct: 550 LAPKVVTMVEQDLSH----SGSFLARFVEAIHYYSALFDSLDASYSEDSPERHVVEQQLL 609

Query: 435 GPRISATLTRIGQPQAN-----CSWGERLEKLGFKAAAISFANHCQARLLLDLF-NDGYR 494
              I   L  +G P         SW E+L + GF+ ++++ +   QA LLL +F +DGY 
Sbjct: 610 SREIRNVLA-VGGPARTGDVKFGSWREKLAQSGFRVSSLAGSAAAQAALLLGMFPSDGYT 652

Query: 495 VEELGNNKLVLGWKSKRLLSTSIW 512
           + E  N  L LGWK   LL+ S W
Sbjct: 670 LIE-ENGALKLGWKDLCLLTASAW 652

BLAST of Lag0029255.1 vs. ExPASy TrEMBL
Match: A0A5A7V767 (Nodulation-signaling pathway 2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1415G00580 PE=3 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 7.3e-215
Identity = 401/527 (76.09%), Postives = 428/527 (81.21%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVDWEPFPGTHVDFHDLFDSM 68
           MALAI+NPF D+ALS+YSTSTNNSDD  HLAGNWNY SP+VDWE F GTH DF D+FDS 
Sbjct: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60

Query: 69  MAVDAPPYPPRAAEVDSGSNCNSGSSPAEDQEEEEEEAKAEDR-EEEEYKGLRLYHLLMA 128
           + ++ PP+                                EDR EE+E+KGLRLYHLL+A
Sbjct: 61  ICIENPPF------------------------------LTEDRDEEDEFKGLRLYHLLIA 120

Query: 129 AADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSGGAGG 188
           AADA+FGDH+S DLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQ LLD A V      
Sbjct: 121 AADAVFGDHRSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLDSAPV------ 180

Query: 189 GSNKPHHHHHHSHHHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRR 248
            SNK HHH+HH H   HRDDH+PTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVA+DRR
Sbjct: 181 -SNKTHHHNHHIH---HRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRR 240

Query: 249 VHIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATCRRSIATVQETGRR 308
           VHIVDYDIMEG QWASLMQAFVS    P APHLRITAISRG  G   RRSI TVQETGRR
Sbjct: 241 VHIVDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISRGANG---RRSIGTVQETGRR 300

Query: 309 LVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFIYRTPESIASF 368
           LVAFAASIGQPFSFHQCKLDSDESFRPSGLKLV+GEALVVNCMLHLPHF YR+PESIASF
Sbjct: 301 LVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASF 360

Query: 369 LSGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARAL 428
           LSGAK+L+PR+VTLVEEEIGHGPT+DGDYKVQFLDSLERYSAIYDSL A  P KNRARAL
Sbjct: 361 LSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARAL 420

Query: 429 VERVFLGPRISATLTRIGQ-------PQANCSWGERLEKLGFKAAAISFANHCQARLLLD 488
           VERVFLGPRISATL RIGQ        + NC WGE+LEK+G K   ISFANHCQARLLL 
Sbjct: 421 VERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLG 480

Query: 489 LFNDGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSCSSSLCSSSDSE 528
           LFNDGYRVEELGNNKLVLGWKSKRLLS SIW SS+ SSS  S SDSE
Sbjct: 481 LFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDSE 481

BLAST of Lag0029255.1 vs. ExPASy TrEMBL
Match: A0A1S3BE54 (nodulation-signaling pathway 2 protein OS=Cucumis melo OX=3656 GN=LOC103488636 PE=3 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 7.3e-215
Identity = 401/527 (76.09%), Postives = 428/527 (81.21%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVDWEPFPGTHVDFHDLFDSM 68
           MALAI+NPF D+ALS+YSTSTNNSDD  HLAGNWNY SP+VDWE F GTH DF D+FDS 
Sbjct: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60

Query: 69  MAVDAPPYPPRAAEVDSGSNCNSGSSPAEDQEEEEEEAKAEDR-EEEEYKGLRLYHLLMA 128
           + ++ PP+                                EDR EE+E+KGLRLYHLL+A
Sbjct: 61  ICIENPPF------------------------------LTEDRDEEDEFKGLRLYHLLIA 120

Query: 129 AADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSGGAGG 188
           AADA+FGDH+S DLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQ LLD A V      
Sbjct: 121 AADAVFGDHRSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLDSAPV------ 180

Query: 189 GSNKPHHHHHHSHHHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRR 248
            SNK HHH+HH H   HRDDH+PTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVA+DRR
Sbjct: 181 -SNKTHHHNHHIH---HRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRR 240

Query: 249 VHIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATCRRSIATVQETGRR 308
           VHIVDYDIMEG QWASLMQAFVS    P APHLRITAISRG  G   RRSI TVQETGRR
Sbjct: 241 VHIVDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISRGANG---RRSIGTVQETGRR 300

Query: 309 LVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFIYRTPESIASF 368
           LVAFAASIGQPFSFHQCKLDSDESFRPSGLKLV+GEALVVNCMLHLPHF YR+PESIASF
Sbjct: 301 LVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASF 360

Query: 369 LSGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARAL 428
           LSGAK+L+PR+VTLVEEEIGHGPT+DGDYKVQFLDSLERYSAIYDSL A  P KNRARAL
Sbjct: 361 LSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARAL 420

Query: 429 VERVFLGPRISATLTRIGQ-------PQANCSWGERLEKLGFKAAAISFANHCQARLLLD 488
           VERVFLGPRISATL RIGQ        + NC WGE+LEK+G K   ISFANHCQARLLL 
Sbjct: 421 VERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLG 480

Query: 489 LFNDGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSCSSSLCSSSDSE 528
           LFNDGYRVEELGNNKLVLGWKSKRLLS SIW SS+ SSS  S SDSE
Sbjct: 481 LFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDSE 481

BLAST of Lag0029255.1 vs. ExPASy TrEMBL
Match: A0A6J1JUC4 (nodulation-signaling pathway 2 protein OS=Cucurbita maxima OX=3661 GN=LOC111489805 PE=3 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 8.1e-214
Identity = 402/526 (76.43%), Postives = 430/526 (81.75%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVDWEPFPGTHVDFHDLFDSM 68
           MA+A+DN F  +A+S+YSTSTNNSDD                         DFHDLFDS+
Sbjct: 1   MAMAVDNAFNGVAVSNYSTSTNNSDD-------------------------DFHDLFDSI 60

Query: 69  MAVDAPPYPPRAAEVDSGSNCNSGSSPAEDQEEEEEEAKAEDREEEEYKGLRLYHLLMAA 128
           M++DA PY P+  EVD G+NCNS S+PAED+EE          EEEE+K LRLYHLLMA 
Sbjct: 61  MSIDALPYSPQVVEVDGGTNCNSPSNPAEDREE----------EEEEFKSLRLYHLLMAV 120

Query: 129 ADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSGGAGGG 188
           ADA+FGD+KSR+LA VIL+RLNELVS SHGTNLERLTAYYAQAFQ LLDCAAVSGG    
Sbjct: 121 ADAMFGDNKSRELAQVILIRLNELVSTSHGTNLERLTAYYAQAFQDLLDCAAVSGGV--- 180

Query: 189 SNKPHHHHHHSHHHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRV 248
            NKP        HHLHRDDHSPTDVLAAFQLLQ+MSPYVKFGHFTANQAILEAVADDRRV
Sbjct: 181 PNKP--------HHLHRDDHSPTDVLAAFQLLQDMSPYVKFGHFTANQAILEAVADDRRV 240

Query: 249 HIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATCRRSIATVQETGRRL 308
           HIVDY+IMEG QWASLMQAFVSRKD PPAPHLRIT ISR   G + RR I TVQETGRRL
Sbjct: 241 HIVDYNIMEGIQWASLMQAFVSRKDSPPAPHLRITVISR---GPSSRRWIGTVQETGRRL 300

Query: 309 VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFIYRTPESIASFL 368
           VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNC+LHLPHF   +PESIASFL
Sbjct: 301 VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNSSPESIASFL 360

Query: 369 SGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALV 428
           +GAKTLNPRLVTLVEEEI HGPT+DGDYKVQFLDSLERYSAIYDSL AG P KNRAR LV
Sbjct: 361 AGAKTLNPRLVTLVEEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLV 420

Query: 429 ERVFLGPRISATLTRIGQPQ-------ANCSWGERLEKLGFKAAAISFANHCQARLLLDL 488
           E+VFLGPRISATL RIGQPQ        NC WGERLEK+G KAAAISFANHCQARLL+DL
Sbjct: 421 EKVFLGPRISATLRRIGQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDL 476

Query: 489 FNDGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSCSSSLCSSSDSE 528
           FNDGYRVEELG+NKLVLGWKSKRLLS SIW SSS SSS  SSSDSE
Sbjct: 481 FNDGYRVEELGSNKLVLGWKSKRLLSVSIWASSSSSSS-SSSSDSE 476

BLAST of Lag0029255.1 vs. ExPASy TrEMBL
Match: A0A6J1FK48 (nodulation-signaling pathway 2 protein OS=Cucurbita moschata OX=3662 GN=LOC111444761 PE=3 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 6.8e-213
Identity = 400/526 (76.05%), Postives = 429/526 (81.56%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVDWEPFPGTHVDFHDLFDSM 68
           MA+A+DN F  +A+SDYSTSTNNS+D                         DFHDLFDS+
Sbjct: 1   MAMAVDNAFNGVAVSDYSTSTNNSND-------------------------DFHDLFDSI 60

Query: 69  MAVDAPPYPPRAAEVDSGSNCNSGSSPAEDQEEEEEEAKAEDREEEEYKGLRLYHLLMAA 128
           M++DA PY P+  E+D G+NCNS S+PAE +EE          EEEE++ LRLYHLLMA 
Sbjct: 61  MSIDALPYSPQVVEIDGGTNCNSPSNPAEGREE----------EEEEFRSLRLYHLLMAV 120

Query: 129 ADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSGGAGGG 188
           ADA+FGDHKSR+LA VIL+RLNELVS SHGTNLER+TAYYAQAFQ LLDCAAVS   GGG
Sbjct: 121 ADAMFGDHKSRELAQVILIRLNELVSTSHGTNLERITAYYAQAFQDLLDCAAVS---GGG 180

Query: 189 SNKPHHHHHHSHHHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRV 248
            NKP        HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRV
Sbjct: 181 PNKP--------HHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRV 240

Query: 249 HIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATCRRSIATVQETGRRL 308
           HIVDY+IMEG QWASLMQA VSRKD PPAPHLRITAISR   G + RR I TVQETGRRL
Sbjct: 241 HIVDYNIMEGIQWASLMQALVSRKDSPPAPHLRITAISR---GPSSRRWIGTVQETGRRL 300

Query: 309 VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFIYRTPESIASFL 368
           VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNC+LHLPHF   +PESIASFL
Sbjct: 301 VAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIASFL 360

Query: 369 SGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALV 428
           +GAKTLNPRLVTLVEEEI HGPT+DGDYKVQFLDSLERYSAIYDSL AG P KNRAR LV
Sbjct: 361 AGAKTLNPRLVTLVEEEIEHGPTIDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLV 420

Query: 429 ERVFLGPRISATLTRIGQPQ-------ANCSWGERLEKLGFKAAAISFANHCQARLLLDL 488
           E+VFLGPRISATL RI QPQ        NC WGERLEK+G KAAAISFANHCQARLL+DL
Sbjct: 421 EKVFLGPRISATLRRIEQPQRAATAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDL 476

Query: 489 FNDGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSCSSSLCSSSDSE 528
           FNDGYRVEELG+NKLVLGWKSKRLLS SIW SSS SSS  SSSDSE
Sbjct: 481 FNDGYRVEELGSNKLVLGWKSKRLLSVSIWASSSLSSS-SSSSDSE 476

BLAST of Lag0029255.1 vs. ExPASy TrEMBL
Match: A0A0A0KDX4 (GRAS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G040600 PE=3 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 8.4e-211
Identity = 399/527 (75.71%), Postives = 424/527 (80.46%), Query Frame = 0

Query: 9   MALAIDNPFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPVVDWEPFPGTHVDFHDLFDSM 68
           MALAI+NP  D+ALS+YSTSTNNSDD  HLAGNWNY SP+VDWE F GTH DF D+FDS 
Sbjct: 1   MALAINNPPKDIALSNYSTSTNNSDDHCHLAGNWNYGSPIVDWETFSGTHNDFLDVFDST 60

Query: 69  MAVDAPPYPPRAAEVDSGSNCNSGSSPAEDQEEEEEEAKAEDR-EEEEYKGLRLYHLLMA 128
           + ++ PP+                                EDR EE+E+KGLRLYHLL A
Sbjct: 61  ICIENPPF------------------------------LTEDRDEEDEFKGLRLYHLLTA 120

Query: 129 AADALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSGGAGG 188
           AADA+ GDHKS DLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQ LLD A V      
Sbjct: 121 AADAVLGDHKSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLDSAPV------ 180

Query: 189 GSNKPHHHHHHSHHHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRR 248
            +NK HHH+HH H    RDDH+PTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVA+DRR
Sbjct: 181 -ANKTHHHNHHIH---QRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRR 240

Query: 249 VHIVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATCRRSIATVQETGRR 308
           VHIVDYDIMEG QWASLMQAFVS    P APHLRITAISRG  G   RRSI TVQETGRR
Sbjct: 241 VHIVDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISRGANG---RRSIGTVQETGRR 300

Query: 309 LVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFIYRTPESIASF 368
           LVAFAASIGQPFSFHQCKLDSDESFRPSGLKLV+GEALVVNCMLHLPHF YR+PESIASF
Sbjct: 301 LVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASF 360

Query: 369 LSGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARAL 428
           LSGAK+L+PR+VTLVEEEIGHGPT+DGDYKVQFLDSLERYSAIYDSL A  P KNRARAL
Sbjct: 361 LSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARAL 420

Query: 429 VERVFLGPRISATLTRIGQ-------PQANCSWGERLEKLGFKAAAISFANHCQARLLLD 488
           VERVFLGPRISATL RIGQ        + NC WGE+LEK+G K   ISFANHCQARLLL 
Sbjct: 421 VERVFLGPRISATLRRIGQRRWSTAEMEDNCLWGEKLEKMGLKTVKISFANHCQARLLLG 478

Query: 489 LFNDGYRVEELGNNKLVLGWKSKRLLSTSIWVSSSCSSSLCSSSDSE 528
           LFNDGYRVEELGNNKLVLGWKSKRLLS SIW SSS SSSL   SDSE
Sbjct: 481 LFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSSSSSSL---SDSE 478

BLAST of Lag0029255.1 vs. TAIR 10
Match: AT4G08250.1 (GRAS family transcription factor )

HSP 1 Score: 422.2 bits (1084), Expect = 6.3e-118
Identity = 251/508 (49.41%), Postives = 317/508 (62.40%), Query Frame = 0

Query: 16  PFTDLALSDYSTSTNNSDDAHHLAGNWNYLSPV-VDWEPFPGTHVDFHDLFDSMMAVDAP 75
           P+ D     +ST T+    A   + N   L+ + +DW+       DF D+ +S+M  +  
Sbjct: 4   PYEDFLDLFFSTHTDPLATAASTSSNGYSLNDLDIDWD------CDFRDVIESIMGDEGA 63

Query: 76  PYPPRAAEV----DSGSNCNSGSSPAEDQEEEEEEAKAEDREEEEYKGLRLYHLLMAAAD 135
              P +  V    D    CNS S+         +     + + +E KGLRL HLL+AAAD
Sbjct: 64  MMEPESEAVPMLHDQEGLCNSASTGL----SVADGVSFGEPKTDESKGLRLVHLLVAAAD 123

Query: 136 ALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSGGAGGGSN 195
           A  G +KSR+L  VIL RL +LVSP   TN+ERL A++      LL+  +V         
Sbjct: 124 ASTGANKSRELTRVILARLKDLVSPGDRTNMERLAAHFTNGLSKLLERDSV--------- 183

Query: 196 KPHHHHHHSHHHLHRDD-HSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVH 255
                        HRDD +   DV++AF+LLQ MSPYV FG+ TA QAILEAV  +RR+H
Sbjct: 184 --------LCPQQHRDDVYDQADVISAFELLQNMSPYVNFGYLTATQAILEAVKYERRIH 243

Query: 256 IVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATCRRSIATVQETGRRLV 315
           IVDYDI EG QWASLMQA VSR  GP A HLRITA+SR   G   ++S+A VQETGRRL 
Sbjct: 244 IVDYDINEGVQWASLMQALVSRNTGPSAQHLRITALSRATNG---KKSVAAVQETGRRLT 303

Query: 316 AFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNCMLHLPHFIYRTPESIASFLS 375
           AFA SIGQPFS+  CKLD++ +F  S LKLVRGEA+V+NCMLHLP F ++TP S+ SFLS
Sbjct: 304 AFADSIGQPFSYQHCKLDTN-AFSTSSLKLVRGEAVVINCMLHLPRFSHQTPSSVISFLS 363

Query: 376 GAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVE 435
            AKTLNP+LVTLV EE+G        Y+  F+D L ++SAI+DSL AG    N AR  VE
Sbjct: 364 EAKTLNPKLVTLVHEEVGLMGNQGFLYR--FMDLLHQFSAIFDSLEAGLSIANPARGFVE 423

Query: 436 RVFLGPRISATLTRI----GQPQANCSWGERLEKLGFKAAAISFANHCQARLLLDLFNDG 495
           RVF+GP ++  LTRI     + ++  SW + LE  GFK   +SF N CQA+LLL LFNDG
Sbjct: 424 RVFIGPWVANWLTRITANDAEVESFASWPQWLETNGFKPLEVSFTNRCQAKLLLSLFNDG 478

Query: 496 YRVEELGNNKLVLGWKSKRLLSTSIWVS 514
           +RVEELG N LVLGWKS+RL+S S W S
Sbjct: 484 FRVEELGQNGLVLGWKSRRLVSASFWAS 478

BLAST of Lag0029255.1 vs. TAIR 10
Match: AT3G54220.1 (GRAS family transcription factor )

HSP 1 Score: 194.5 bits (493), Expect = 2.1e-49
Identity = 148/453 (32.67%), Postives = 233/453 (51.43%), Query Frame = 0

Query: 70  AVDAPPYPPRAAEVDSGSNCNSGSSPAEDQEEEEEEAKAEDREEEEYKGLRLYHLLMAAA 129
           + DAPP P            N+    AE   E +EE K + ++EE   GL L  LL+  A
Sbjct: 246 STDAPPQPETVTATVPAVQTNT----AEALRERKEEIKRQKQDEE---GLHLLTLLLQCA 305

Query: 130 DALFGDHKSRDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQGLLDCAAVSGGAGGGS 189
           +A+  D+     A+ +L+ +++L +P +GT+ +R+ AY+++A    L    ++   G  +
Sbjct: 306 EAVSADNLEE--ANKLLLEISQLSTP-YGTSAQRVAAYFSEAMSARL----LNSCLGIYA 365

Query: 190 NKPHHHHHHSHHHLHRDDHSPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVH 249
             P      +H            +++AFQ+   +SP VKF HFTANQAI EA   +  VH
Sbjct: 366 ALPSRWMPQTH---------SLKMVSAFQVFNGISPLVKFSHFTANQAIQEAFEKEDSVH 425

Query: 250 IVDYDIMEGSQWASLMQAFVSRKDGPPAPHLRITAISRGGGGATCRRSIATVQETGRRLV 309
           I+D DIM+G QW  L     SR  GP  PH+R+T +           S+  +Q TG+RL 
Sbjct: 426 IIDLDIMQGLQWPGLFHILASRPGGP--PHVRLTGLG---------TSMEALQATGKRLS 485

Query: 310 AFAASIGQPFSFHQCKL-DSDESFRPSGLKLVRGEALVVNCMLHLPHFIYRTPESIASFL 369
            FA  +G PF F  C L +   +     L + + EA+ V+    L H +Y    S A  L
Sbjct: 486 DFADKLGLPFEF--CPLAEKVGNLDTERLNVRKREAVAVH---WLQHSLYDVTGSDAHTL 545

Query: 370 SGAKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALV 429
              + L P++VT+VE+++ H     G +  +F++++  YSA++DSLGA + +++  R +V
Sbjct: 546 WLLQRLAPKVVTVVEQDLSHA----GSFLGRFVEAIHYYSALFDSLGASYGEESEERHVV 605

Query: 430 ERVFLGPRISATLTRIGQPQANC-----SWGERLEKLGFKAAAISFANHCQARLLLDLF- 489
           E+  L   I   L  +G P  +      SW E++++ GFK  +++     QA LLL +F 
Sbjct: 606 EQQLLSKEIRNVLA-VGGPSRSGEVKFESWREKMQQCGFKGISLAGNAATQATLLLGMFP 653

Query: 490 NDGYRVEELGNNKLVLGWKSKRLLSTSIWVSSS 516
           +DGY + +  N  L LGWK   LL+ S W   S
Sbjct: 666 SDGYTLVD-DNGTLKLGWKDLSLLTASAWTPRS 653

BLAST of Lag0029255.1 vs. TAIR 10
Match: AT5G41920.1 (GRAS family transcription factor )

HSP 1 Score: 181.0 bits (458), Expect = 2.4e-45
Identity = 133/422 (31.52%), Postives = 205/422 (48.58%), Query Frame = 0

Query: 93  SSPAEDQEEEEEEAKAEDREEEEYKGLRLYHLLMAAADALFGDHKSRDLAHVILVRLNEL 152
           SS      +   E   E  E +    ++L  LL+  A+ +  DH     A  +L  ++E+
Sbjct: 12  SSDDPSSAKRRIEFPEETLENDGAAAIKLLSLLLQCAEYVATDHLRE--ASTLLSEISEI 71

Query: 153 VSPSHGTNLERLTAYYAQAFQGLLDCAAVSGGAGGGSNKPHHHHHHSHHHLHRDDHSPTD 212
            SP  G++ ER+ AY+AQA Q  +  + +SG     S KP                    
Sbjct: 72  CSP-FGSSPERVVAYFAQALQTRVISSYLSGACSPLSEKP------------LTVVQSQK 131

Query: 213 VLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHIVDYDIMEGSQWASLMQAFVSRK 272
           + +A Q    +SP +KF HFTANQAI +A+  +  VHI+D D+M+G QW +L     SR 
Sbjct: 132 IFSALQTYNSVSPLIKFSHFTANQAIFQALDGEDSVHIIDLDVMQGLQWPALFHILASRP 191

Query: 273 DGPPAPHLRITAISRGGGGATCRRSIATVQETGRRLVAFAASIGQPFSFHQCKLDSDESF 332
                   ++ +I   G G+    S   +  TGRRL  FA+S+  PF FH  +       
Sbjct: 192 -------RKLRSIRITGFGS----SSDLLASTGRRLADFASSLNLPFEFHPIEGIIGNLI 251

Query: 333 RPSGLKLVRGEALVVNCMLHLPHFIYRTPESIASFLSGAKTLNPRLVTLVEEEIGHGPTM 392
            PS L   +GEA+VV+ M    H +Y    +    L   + L P L+T+VE+E+ +    
Sbjct: 252 DPSQLATRQGEAVVVHWM---QHRLYDVTGNNLETLEILRRLKPNLITVVEQELSYDD-- 311

Query: 393 DGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVERVFLGPRISATLTRIGQPQANCS 452
            G +  +F+++L  YSA++D+LG G  +++  R  VE++ LG  I   +   G  +    
Sbjct: 312 GGSFLGRFVEALHYYSALFDALGDGLGEESGERFTVEQIVLGTEIRNIVAHGGGRRKRMK 371

Query: 453 WGERLEKLGFKAAAISFANHCQARLLLDLFN-DGYRVEELGNNKLVLGWKSKRLLSTSIW 512
           W E L ++GF+  ++      QA LLL +   +GY + E  N  L LGWK   LL+ S W
Sbjct: 372 WKEELSRVGFRPVSLRGNPATQAGLLLGMLPWNGYTLVE-ENGTLRLGWKDLSLLTASAW 401

Query: 513 VS 514
            S
Sbjct: 432 KS 401

BLAST of Lag0029255.1 vs. TAIR 10
Match: AT1G55580.1 (GRAS family transcription factor )

HSP 1 Score: 180.6 bits (457), Expect = 3.2e-45
Identity = 118/328 (35.98%), Postives = 171/328 (52.13%), Query Frame = 0

Query: 203 LHRDDHSPTDVLAAFQL-LQEMSPYVKFGHFTANQAILEAVA--DDRRVHIVDYDIMEGS 262
           L R  ++ +D  + + L L +++P+++FGH TANQAIL+A    D+  +HI+D DI +G 
Sbjct: 131 LFRTKNNNSDFESCYYLWLNQLTPFIRFGHLTANQAILDATETNDNGALHILDLDISQGL 190

Query: 263 QWASLMQAFVSRKDGP--PAPHLRITAISRGGGGATCRRSIATVQETGRRLVAFAASIGQ 322
           QW  LMQA   R   P  P P LRIT          C R +  +  TG RL  FA S+G 
Sbjct: 191 QWPPLMQALAERSSNPSSPPPSLRITG---------CGRDVTGLNRTGDRLTRFADSLGL 250

Query: 323 PFSFHQCKLDSDE------SFRPSGLKLVRGEALVVNCMLHLPHFIYRTP-ESIASFLSG 382
            F FH   +  ++        R   L  V+GE + VNC +H  H I+    + I  FLS 
Sbjct: 251 QFQFHTLVIVEEDLAGLLLQIRLLALSAVQGETIAVNC-VHFLHKIFNDDGDMIGHFLSA 310

Query: 383 AKTLNPRLVTLVEEEIGHGPTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVER 442
            K+LN R+VT+ E E  HG   D  +  +F ++++ Y AI+DSL A  P  +R R  +E+
Sbjct: 311 IKSLNSRIVTMAEREANHG---DHSFLNRFSEAVDHYMAIFDSLEATLPPNSRERLTLEQ 370

Query: 443 VFLGPRI----SATLTRIGQPQANCS-WGERLEKLGFKAAAISFANHCQARLLLDLF--N 502
            + G  I    +A  T   Q       W E +++ GF    I      QA+LLL L   +
Sbjct: 371 RWFGKEILDVVAAEETERKQRHRRFEIWEEMMKRFGFVNVPIGSFALSQAKLLLRLHYPS 430

Query: 503 DGYRVEELGNNKLVLGWKSKRLLSTSIW 512
           +GY ++ L NN L LGW+++ L S S W
Sbjct: 431 EGYNLQFL-NNSLFLGWQNRPLFSVSSW 444

BLAST of Lag0029255.1 vs. TAIR 10
Match: AT3G03450.1 (RGA-like 2 )

HSP 1 Score: 172.9 bits (437), Expect = 6.6e-43
Identity = 131/429 (30.54%), Postives = 211/429 (49.18%), Query Frame = 0

Query: 100 EEEEEEAKAEDREEEEYKGLRLYHLLMAAADALFGDHKSRDLAHVILVRLNELVSPSHGT 159
           E  +E  ++    + +  G+RL H L+A A+A+    ++ +LA  ++ R+  L     G 
Sbjct: 159 ESSDESTRSVVLVDSQETGVRLVHALVACAEAI--HQENLNLADALVKRVGTLAGSQAGA 218

Query: 160 NLERLTAYYAQAFQGLLDCAAVSGGAGGGSNKPHHHHHHSHHHLHRDDHSPTDVLAA--- 219
            + ++  Y+AQA                               ++RD  + TDV AA   
Sbjct: 219 -MGKVATYFAQALA---------------------------RRIYRDYTAETDVCAAVNP 278

Query: 220 -FQLLQEM-----SPYVKFGHFTANQAILEAVADDRRVHIVDYDIMEGSQWASLMQAFVS 279
            F+ + EM      PY+KF HFTANQAILEAV   RRVH++D  + +G QW +LMQA   
Sbjct: 279 SFEEVLEMHFYESCPYLKFAHFTANQAILEAVTTARRVHVIDLGLNQGMQWPALMQALAL 338

Query: 280 RKDGPPAPHLRITAISRGGGGATCRRSIATVQETGRRLVAFAASIGQPFSFHQCKLDSDE 339
           R  GPP+   R+T I     G     +  ++Q+ G +L  FA ++G  F F     +S  
Sbjct: 339 RPGGPPS--FRLTGI-----GPPQTENSDSLQQLGWKLAQFAQNMGVEFEFKGLAAESLS 398

Query: 340 SFRPSGLKL-VRGEALVVNCMLHLPHFIYRTPESIASFLSGAKTLNPRLVTLVEEEIGHG 399
              P   +     E LVVN +  L   + R+  SI   L+  K + P +VT+VE+E  H 
Sbjct: 399 DLEPEMFETRPESETLVVNSVFELHRLLARS-GSIEKLLNTVKAIKPSIVTVVEQEANHN 458

Query: 400 PTMDGDYKVQFLDSLERYSAIYDSLGAGFPKKNRARALVERVFLGPRISATLT-----RI 459
             +  D   +F ++L  YS+++DSL   +   ++ R + E V+LG +I   +      R+
Sbjct: 459 GIVFLD---RFNEALHYYSSLFDSLEDSYSLPSQDRVMSE-VYLGRQILNVVAAEGSDRV 518

Query: 460 GQPQANCSWGERLEKLGFKAAAISFANHCQARLLLDLF--NDGYRVEELGNNKLVLGWKS 512
            + +    W  R++  GF    +  +   QA +LL L+   DGYRVEE  +  L++GW++
Sbjct: 519 ERHETAAQWRIRMKSAGFDPIHLGSSAFKQASMLLSLYATGDGYRVEE-NDGCLMIGWQT 544