Lag0019953.1 (mRNA) Sponge gourd (AG‐4) v1

Overview
NameLag0019953.1
TypemRNA
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionProtein-serine/threonine phosphatase
Locationchr5: 46920239 .. 46923443 (+)
Sequence length1308
RNA-Seq ExpressionLag0019953.1
SyntenyLag0019953.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTACCACGTGTCCTCACAGCATGAAATGGAGGTCGGGTGGGGCAGGTTTTGCAGCTGCCCGTAGAATTAGAATTGCCCGAGAGCCTCTCTTTTTTCCTGCTCGACGAAGAACTGGGTCGGAAAAAGGGAAAAATAATTTAATTTTTCCCACAGAGGAAAACACACAGCGACACGTTTTCTTCTCAGCGAAATTCCGCACCGCATTCCATTACTTCCTTATATTACACTTCGCCGATGATGATTGTCCTCACAGAGAGATTCTTGTCGGCCTGTTCCTGCTTCGGACCATTCCGATGACGGACGTTTACTGTAGAATGCCAAGCGAAGATGATGGAGATTCGCCGGCCAAATGCCGAGAGCGCCGGCGCCGTAGAATAGAGATGAGGCGATTAGCAACAGTACCGTCCGCGAATCCCTCGTCCTCGACCAGTCACCACCGGAAGGAGAATCAGACTGAGACTTCCGGTTTCGAGAAGAAGCGTGTTCGGAAGACGGACGGCGACGGTACTCCGGAGATTTCATCATCTTCGTCATCGGGGGAGGACGTGAAGACTGTAGTGGCTTCACCGTCGGTACCTCAGCCGGTGTTCGGGATGATGTCGGTGTCCGGGAGGTCACGTGAGATGGAGGACGCAGTATCGGTGAGCACGTGGCTTCTTGGTCCAGAAAATTGCCGGCGGCTACCGGTGCATTTCTTCGCCGTCTACGATGGACATGGAGGCCCTCACGTAAAGCCCTAATAGTAAAGTTTAAAATTTATTTTTCCACAAATAAGTAAATAGAAAAAAAAAAATTGGACGGCGGAAATTACCATTTAGATCACGTTAAATCATTCTCTATAGTAGCGGAAAAGGGGGTTATTATCGTCATTGTGCTGCTATAATTTGCTTTGCTTTATTCGATGGGTACAGGTGGCAGCCCTATGTAGGGAGAAGATGCACGTGTTAGTACGGGAGGAATTTTCGCGCGTGGTGGGTACGCGCCAAGAAACTGAGAGTGGCGGCGGAGGAAGCAGCACGGCGGATGAGGTTAAGGTGGAGGAGGAGGCGACTTGGAGAAGAGTGATGCGGCGGAGCTTTGAGAGAATGGATGAAGTGGCACTCAGTACTTGTGCCTGTGGGAGCGTCGGAGGCCAGTGTGGTTGCCATCCAATGGAGGTGGCTCTCGGCGGCTCCACCGCTGTCGTCGCCGTGCTCACGCCGGATCACATAATCGTCGCCAACTGCGGTGATTCACGCGCCGTCCTCTGCCGAGGAGGAAGGGCCATTCCGCTTTCCAATGATCACAAGGTTTACTCTCTCTCTCACTCTCTCTTTCTCTCCCGAAACTGTTGCTGTAATGATCTGTTTGCTCGACACGTGCTATGCTGTCAATTTTTGACGAGATGATAACGATCAAGGGTTTGTTTGTCTAATCTTGCTGCTTTTGTGATGGGCTAATGGACCATTTTAGTCTAAAAGTGCTTAACTTATGCTTTGATTTTAGATTGATTAGGTTTCCTTTAGTTTCTCATTCATTTGGCCATTTATTCTTCTTTTTTCCATTCTTTACTAATTTTGGGACAGAAGCATAATGCTTTGCAAATTCTGTCCCATCCTTAACAGCTATTAATTCCTCGGAGTTATGTTATCTTATGGATAGTGATCCTGACGTGTGATAGTTATTTTATTGACACTTGTTGCATGACTGTGGCTTAATTCTTTTGGTTTTCGATCTGGGTTTAAATTTGTTAGCCCACCTGAATATATGCGAGAAAAAATGGTTGATGAAATTTTAGATATTTAAAAGTTTCTGAAAGTGTGGAATCGGGTATCATTTCGCAAGTTACCATTGACTATTCGTTATCATCTACTTTTCTGTGTGCGCGTTACAAAGAGTTGTTTGATCATTGATGGGTCTAGCTATCTAATCTTATGTGATAAAAAAAAGTTAAACGCTGTGGCCTTGCTGCTTAAATGTTTGAGTATTTCCATGGAACTTGGTTTTTGTTTTTTGTTTTTTGTTTTTTTTGTCTAAATGTGTTTCAGATTCCTATGGGCATTCATCTCTCATACCTGTCCCACAGTTTTCCATAAGGAAAGTACATTTTAGATGAATAGAATGTTGGTGACAAGAACATATAATGATTATGTGTTCTAATAAGTAATAGTATTGGTCTATTTAATTTCACTGTGTATTCTTGCACGATATCTTTTCCAACCAGTACGCGTTTCATGAAGAACATATAATATTCTCTAACTATTTTGCTTAACCTAATTCTCAGCCTGATAGAATTGATGAACTAGCAAGGATCGAAGCTGCTGGGGGACGGGTGATTTTTGTTAATGGAGCTCGGGTTGAAGGCATCCTTGCAATGTCCCGTGCAATAGGTAATAACTACTGCTTCTGTGAACTATGACCACGATATTATCTGCTTTTGTGAAGGTGCTTAAAGTTAAACATTCCAACGTAAATTATAATTCTTCCAGTGTATGATTATTTCATAAGGCCATCTAAAGAAGTCTGATTTTGTTTTCAAGGAATTAAGAAAAGTGATCACTTTGACTCTGTCTAGGCAATTATGAAGTGGACCTTTCTTGTTAGGCACAAACCAATTGTGGTCATGTTTAGTTTCGGCACAGTTCTAAGTGATCAACTTTTCCAAATCCTGCATTCTTTTCTAAGAATTCATACATTCAAGGATTTCCTAAGAATTTGTTTGTCATACCCTCTGTCCCTAGTGCAATATATGTAAACACTCCATAGAAGGGAATGTATGAAACTTTAAATGATTGAAGGGATAGGTGAAGCTACGGTTACGTAGTGGTGTTGATCAAACACAATTTCGTGTTATTAGGTTGAGTCCATGGATAATTCACAAATTGCTAAATGGAGCAGCTATGCACATTTGGAATTTACATTTAATTTGACAAAGGAGGTCTTTATGCTCTGAAATTCACTGTGTTTGCATTCATTTATAACATTCTCTAATCTCCACAAGATCATTACTTCTTTGAATCTGAAGTAAACACTATTTACTTTGTTCTCTCTCCCCCTCTTCTCTTGGAGCATTGAACTTGATGAAGCCTCATGATACTTCTGTGTAATTGGTTCCCTGACAACATTGCAGGGCAGAAATATGAAGTAATTTCTGAACGAATGCATGATGTTGCATTTACTCATCAACAGATTCTTGGCATGATTGAATGGTCGTGTGAATAA

mRNA sequence

ATGGGTACCACGTGTCCTCACAGCATGAAATGGAGGTCGGGTGGGGCAGGTTTTGCAGCTGCCCGTAGAATTAGAATTGCCCGAGAGCCTCTCTTTTTTCCTGCTCGACGAAGAACTGGGTCGGAAAAAGGGAAAAATAATTTAATTTTTCCCACAGAGGAAAACACACAGCGACACGTTTTCTTCTCAGCGAAATTCCGCACCGCATTCCATTACTTCCTTATATTACACTTCGCCGATGATGATTGTCCTCACAGAGAGATTCTTGTCGGCCTGTTCCTGCTTCGGACCATTCCGATGACGGACGTTTACTGTAGAATGCCAAGCGAAGATGATGGAGATTCGCCGGCCAAATGCCGAGAGCGCCGGCGCCGTAGAATAGAGATGAGGCGATTAGCAACAGTACCGTCCGCGAATCCCTCGTCCTCGACCAGTCACCACCGGAAGGAGAATCAGACTGAGACTTCCGGTTTCGAGAAGAAGCGTGTTCGGAAGACGGACGGCGACGGTACTCCGGAGATTTCATCATCTTCGTCATCGGGGGAGGACGTGAAGACTGTAGTGGCTTCACCGTCGGTACCTCAGCCGGTGTTCGGGATGATGTCGGTGTCCGGGAGGTCACGTGAGATGGAGGACGCAGTATCGGTGAGCACGTGGCTTCTTGGTCCAGAAAATTGCCGGCGGCTACCGGTGCATTTCTTCGCCGTCTACGATGGACATGGAGGCCCTCACGTGGCAGCCCTATGTAGGGAGAAGATGCACGTGTTAGTACGGGAGGAATTTTCGCGCGTGGTGGGTACGCGCCAAGAAACTGAGAGTGGCGGCGGAGGAAGCAGCACGGCGGATGAGGTTAAGGTGGAGGAGGAGGCGACTTGGAGAAGAGTGATGCGGCGGAGCTTTGAGAGAATGGATGAAGTGGCACTCAGTACTTGTGCCTGTGGGAGCGTCGGAGGCCAGTGTGGTTGCCATCCAATGGAGGTGGCTCTCGGCGGCTCCACCGCTGTCGTCGCCGTGCTCACGCCGGATCACATAATCGTCGCCAACTGCGGTGATTCACGCGCCGTCCTCTGCCGAGGAGGAAGGGCCATTCCGCTTTCCAATGATCACAAGCCTGATAGAATTGATGAACTAGCAAGGATCGAAGCTGCTGGGGGACGGGTGATTTTTGTTAATGGAGCTCGGGTTGAAGGCATCCTTGCAATGTCCCGTGCAATAGGGCAGAAATATGAAGTAATTTCTGAACGAATGCATGATGTTGCATTTACTCATCAACAGATTCTTGGCATGATTGAATGGTCGTGTGAATAA

Coding sequence (CDS)

ATGGGTACCACGTGTCCTCACAGCATGAAATGGAGGTCGGGTGGGGCAGGTTTTGCAGCTGCCCGTAGAATTAGAATTGCCCGAGAGCCTCTCTTTTTTCCTGCTCGACGAAGAACTGGGTCGGAAAAAGGGAAAAATAATTTAATTTTTCCCACAGAGGAAAACACACAGCGACACGTTTTCTTCTCAGCGAAATTCCGCACCGCATTCCATTACTTCCTTATATTACACTTCGCCGATGATGATTGTCCTCACAGAGAGATTCTTGTCGGCCTGTTCCTGCTTCGGACCATTCCGATGACGGACGTTTACTGTAGAATGCCAAGCGAAGATGATGGAGATTCGCCGGCCAAATGCCGAGAGCGCCGGCGCCGTAGAATAGAGATGAGGCGATTAGCAACAGTACCGTCCGCGAATCCCTCGTCCTCGACCAGTCACCACCGGAAGGAGAATCAGACTGAGACTTCCGGTTTCGAGAAGAAGCGTGTTCGGAAGACGGACGGCGACGGTACTCCGGAGATTTCATCATCTTCGTCATCGGGGGAGGACGTGAAGACTGTAGTGGCTTCACCGTCGGTACCTCAGCCGGTGTTCGGGATGATGTCGGTGTCCGGGAGGTCACGTGAGATGGAGGACGCAGTATCGGTGAGCACGTGGCTTCTTGGTCCAGAAAATTGCCGGCGGCTACCGGTGCATTTCTTCGCCGTCTACGATGGACATGGAGGCCCTCACGTGGCAGCCCTATGTAGGGAGAAGATGCACGTGTTAGTACGGGAGGAATTTTCGCGCGTGGTGGGTACGCGCCAAGAAACTGAGAGTGGCGGCGGAGGAAGCAGCACGGCGGATGAGGTTAAGGTGGAGGAGGAGGCGACTTGGAGAAGAGTGATGCGGCGGAGCTTTGAGAGAATGGATGAAGTGGCACTCAGTACTTGTGCCTGTGGGAGCGTCGGAGGCCAGTGTGGTTGCCATCCAATGGAGGTGGCTCTCGGCGGCTCCACCGCTGTCGTCGCCGTGCTCACGCCGGATCACATAATCGTCGCCAACTGCGGTGATTCACGCGCCGTCCTCTGCCGAGGAGGAAGGGCCATTCCGCTTTCCAATGATCACAAGCCTGATAGAATTGATGAACTAGCAAGGATCGAAGCTGCTGGGGGACGGGTGATTTTTGTTAATGGAGCTCGGGTTGAAGGCATCCTTGCAATGTCCCGTGCAATAGGGCAGAAATATGAAGTAATTTCTGAACGAATGCATGATGTTGCATTTACTCATCAACAGATTCTTGGCATGATTGAATGGTCGTGTGAATAA

Protein sequence

MGTTCPHSMKWRSGGAGFAAARRIRIAREPLFFPARRRTGSEKGKNNLIFPTEENTQRHVFFSAKFRTAFHYFLILHFADDDCPHREILVGLFLLRTIPMTDVYCRMPSEDDGDSPAKCRERRRRRIEMRRLATVPSANPSSSTSHHRKENQTETSGFEKKRVRKTDGDGTPEISSSSSSGEDVKTVVASPSVPQPVFGMMSVSGRSREMEDAVSVSTWLLGPENCRRLPVHFFAVYDGHGGPHVAALCREKMHVLVREEFSRVVGTRQETESGGGGSSTADEVKVEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVLTPDHIIVANCGDSRAVLCRGGRAIPLSNDHKPDRIDELARIEAAGGRVIFVNGARVEGILAMSRAIGQKYEVISERMHDVAFTHQQILGMIEWSCE
Homology
BLAST of Lag0019953.1 vs. NCBI nr
Match: KAG7013190.1 (Subtilisin-like protease SBT1.3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 558.5 bits (1438), Expect = 5.0e-155
Identity = 307/417 (73.62%), Postives = 329/417 (78.90%), Query Frame = 0

Query: 53   EENTQRHVFFSAKFRTAFHYFLI-LHFADDDCPHREILVGLFLLRTIPMTDVYCRMPSED 112
            EE T+RHVFFSAKF  AFHY LI LHFA+DD  +REI+ G FL RTIPMT+VYCRMPS+D
Sbjct: 780  EEETKRHVFFSAKFLNAFHYLLILLHFANDDNSYREIIDGEFLFRTIPMTEVYCRMPSDD 839

Query: 113  DGDSPAKCRERRRRRIEMRRLATVPSANPSSSTS---HHRKENQTETSGFEKKRVRKTDG 172
            D DSPAKCRERRRRRIEMRRLA VPSA PS S S   HHRKENQTE+SG EKKRVRKTDG
Sbjct: 840  DIDSPAKCRERRRRRIEMRRLAAVPSAKPSPSPSTSHHHRKENQTESSGLEKKRVRKTDG 899

Query: 173  DGTPEISSSSSSGEDVKTVVASPSVPQPVFGMMSVSGRSREMEDAVSVSTWLLGPENCRR 232
              TPE+ +SSSSGEDV  VVAS  VPQPVFGMMSVSGRSREMEDAVSVST L+GPE  RR
Sbjct: 900  GDTPEVLTSSSSGEDVNAVVASSLVPQPVFGMMSVSGRSREMEDAVSVSTCLIGPE--RR 959

Query: 233  LPVHFFAVYDGHGGPHVAALCREKMHVLVREEFSRVVGTRQETESGGGGSSTADEVKVEE 292
            LPVHFFAVYDGHGGPHVA LC EKMHVLV+EE +RVV TR+ETES GGGSS A     EE
Sbjct: 960  LPVHFFAVYDGHGGPHVATLCMEKMHVLVQEELARVVNTREETESIGGGSSAA-----EE 1019

Query: 293  EATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVLTPDHIIVAN 352
            EATWRRVMRRSFERMDEVAL+TCACGSVGGQC CHPMEVALGGSTAVVAVLTP+HIIVAN
Sbjct: 1020 EATWRRVMRRSFERMDEVALNTCACGSVGGQCACHPMEVALGGSTAVVAVLTPEHIIVAN 1079

Query: 353  CGDSRAVLCRGGRAIPLSNDHK-------------------------------------P 412
            CGDSRAVLCRGGRAIPLSNDHK                                     P
Sbjct: 1080 CGDSRAVLCRGGRAIPLSNDHKGYEFVIVMLWMAILTFYSYLYDTCCATGAMGISSSISP 1139

Query: 413  DRIDELARIEAAGGRVIFVNGARVEGILAMSRAIGQKY---EVISERMHDVAFTHQQ 426
            DRIDELARIEAAGG+VIF+NGARV+GILAMSRAIG KY    VISE   ++ FT ++
Sbjct: 1140 DRIDELARIEAAGGQVIFINGARVKGILAMSRAIGDKYLKSVVISE--PEITFTKRE 1187

BLAST of Lag0019953.1 vs. NCBI nr
Match: XP_008439132.1 (PREDICTED: probable protein phosphatase 2C 75 isoform X1 [Cucumis melo])

HSP 1 Score: 545.0 bits (1403), Expect = 5.7e-151
Identity = 283/329 (86.02%), Postives = 298/329 (90.58%), Query Frame = 0

Query: 100 MTDVYCRMPSEDDGDSPAKCRERRRRRIEMRRLATVPSANPSSSTSHHRKENQTETSGFE 159
           MT+V+CRMPS DD DSPAKCRERRRRRIEMRRLATV SANPS ST+HHRKENQT TSGFE
Sbjct: 1   MTEVHCRMPSGDDEDSPAKCRERRRRRIEMRRLATVSSANPSPSTAHHRKENQTGTSGFE 60

Query: 160 KKRVRKTDGDGTPEISSSSSSGEDVKTVVASPSVPQPVFGMMSVSGRSREMEDAVSVSTW 219
           KKR +KT+GDG PEISSSSSSGEDVKTV ASPSVPQPVFGMMSVSGRSREMEDAVSVST+
Sbjct: 61  KKRGQKTEGDGNPEISSSSSSGEDVKTVKASPSVPQPVFGMMSVSGRSREMEDAVSVSTF 120

Query: 220 LLGPENCRRLPVHFFAVYDGHGGPHVAALCREKMHVLVREEFSRVVGTRQETESGGGGSS 279
           ++G EN RR  VHFFAVYDGHGGPHVAALCREKMHV V+EEFSRV+ TR+E ESGGGGSS
Sbjct: 121 VVGSENFRRQVVHFFAVYDGHGGPHVAALCREKMHVFVQEEFSRVISTREENESGGGGSS 180

Query: 280 TADEVKVEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL 339
             +EV  EEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL
Sbjct: 181 AGEEVNFEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL 240

Query: 340 TPDHIIVANCGDSRAVLCRGGRAIPLSNDHKPDRIDELARIEAAGGRVIFVNGARVEGIL 399
           TPDHIIVANCGDSRAVLCRGGRAIPLS DHKPDR DELARIEAAGGRVIFVNGARVEGIL
Sbjct: 241 TPDHIIVANCGDSRAVLCRGGRAIPLSIDHKPDRNDELARIEAAGGRVIFVNGARVEGIL 300

Query: 400 AMSRAIGQKY---EVISERMHDVAFTHQQ 426
           AMSRAIG KY    VISE   +V FT ++
Sbjct: 301 AMSRAIGDKYLKSVVISE--PEVTFTKRE 327

BLAST of Lag0019953.1 vs. NCBI nr
Match: XP_008439133.1 (PREDICTED: probable protein phosphatase 2C 75 isoform X2 [Cucumis melo])

HSP 1 Score: 540.0 bits (1390), Expect = 1.8e-149
Identity = 275/309 (89.00%), Postives = 287/309 (92.88%), Query Frame = 0

Query: 100 MTDVYCRMPSEDDGDSPAKCRERRRRRIEMRRLATVPSANPSSSTSHHRKENQTETSGFE 159
           MT+V+CRMPS DD DSPAKCRERRRRRIEMRRLATV SANPS ST+HHRKENQT TSGFE
Sbjct: 1   MTEVHCRMPSGDDEDSPAKCRERRRRRIEMRRLATVSSANPSPSTAHHRKENQTGTSGFE 60

Query: 160 KKRVRKTDGDGTPEISSSSSSGEDVKTVVASPSVPQPVFGMMSVSGRSREMEDAVSVSTW 219
           KKR +KT+GDG PEISSSSSSGEDVKTV ASPSVPQPVFGMMSVSGRSREMEDAVSVST+
Sbjct: 61  KKRGQKTEGDGNPEISSSSSSGEDVKTVKASPSVPQPVFGMMSVSGRSREMEDAVSVSTF 120

Query: 220 LLGPENCRRLPVHFFAVYDGHGGPHVAALCREKMHVLVREEFSRVVGTRQETESGGGGSS 279
           ++G EN RR  VHFFAVYDGHGGPHVAALCREKMHV V+EEFSRV+ TR+E ESGGGGSS
Sbjct: 121 VVGSENFRRQVVHFFAVYDGHGGPHVAALCREKMHVFVQEEFSRVISTREENESGGGGSS 180

Query: 280 TADEVKVEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL 339
             +EV  EEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL
Sbjct: 181 AGEEVNFEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL 240

Query: 340 TPDHIIVANCGDSRAVLCRGGRAIPLSNDHKPDRIDELARIEAAGGRVIFVNGARVEGIL 399
           TPDHIIVANCGDSRAVLCRGGRAIPLS DHKPDR DELARIEAAGGRVIFVNGARVEGIL
Sbjct: 241 TPDHIIVANCGDSRAVLCRGGRAIPLSIDHKPDRNDELARIEAAGGRVIFVNGARVEGIL 300

Query: 400 AMSRAIGQK 409
           AMSRAIG K
Sbjct: 301 AMSRAIGMK 309

BLAST of Lag0019953.1 vs. NCBI nr
Match: XP_038883683.1 (probable protein phosphatase 2C 75 isoform X1 [Benincasa hispida])

HSP 1 Score: 539.3 bits (1388), Expect = 3.1e-149
Identity = 284/331 (85.80%), Postives = 300/331 (90.63%), Query Frame = 0

Query: 100 MTDVYCRMPSEDDGDSPAKCRERRRRRIEMRRLATVPSANPSSSTSHHRKENQTETSGFE 159
           MT+VY  MPSEDD DSPAKCRERRRRRIEMRRLATV SANPSSST+HHRKENQT +SGFE
Sbjct: 1   MTEVYSIMPSEDDEDSPAKCRERRRRRIEMRRLATVSSANPSSSTTHHRKENQTGSSGFE 60

Query: 160 KKRVRKTDGDGTPEISSSSSSGEDVKTVVASPSVPQPVFGMMSVSGRSREMEDAVSVSTW 219
           KKR RKTDGDG PEISSSSSSGEDVKTV AS SVPQPVFGMMSVSGRSREMEDAVSVST 
Sbjct: 61  KKRGRKTDGDGNPEISSSSSSGEDVKTVRASASVPQPVFGMMSVSGRSREMEDAVSVSTC 120

Query: 220 LLGPENCRRLPVHFFAVYDGHGGPHVAALCREKMHVLVREEFSRVVGTRQETESGGGGSS 279
           +LGPEN RR  VHFFAVYDGHGGPHVAALCREKMHV V+EEFSRV+ TR+E ESGGGGS+
Sbjct: 121 VLGPENFRRQLVHFFAVYDGHGGPHVAALCREKMHVFVQEEFSRVICTREEGESGGGGST 180

Query: 280 TAD--EVKVEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVA 339
            A+  E++ EEEATWRRVMRRSFERMDEVALSTCACGSVGG+CGCHPMEVALGGSTAVVA
Sbjct: 181 AAEGVELEEEEEATWRRVMRRSFERMDEVALSTCACGSVGGKCGCHPMEVALGGSTAVVA 240

Query: 340 VLTPDHIIVANCGDSRAVLCRGGRAIPLSNDHKPDRIDELARIEAAGGRVIFVNGARVEG 399
           VLTPDHIIVANCGDSRAVLCRGGRAIPLSNDHKPDR DELARIEAAGGRVIFVNGARVEG
Sbjct: 241 VLTPDHIIVANCGDSRAVLCRGGRAIPLSNDHKPDRNDELARIEAAGGRVIFVNGARVEG 300

Query: 400 ILAMSRAIGQKY---EVISERMHDVAFTHQQ 426
           ILAMSRAIG KY    VISE   ++ FT ++
Sbjct: 301 ILAMSRAIGDKYLKSVVISE--PEITFTKRE 329

BLAST of Lag0019953.1 vs. NCBI nr
Match: XP_004148120.2 (probable protein phosphatase 2C 75 [Cucumis sativus] >KGN57314.1 hypothetical protein Csa_009492 [Cucumis sativus])

HSP 1 Score: 537.3 bits (1383), Expect = 1.2e-148
Identity = 281/329 (85.41%), Postives = 294/329 (89.36%), Query Frame = 0

Query: 100 MTDVYCRMPSEDDGDSPAKCRERRRRRIEMRRLATVPSANPSSSTSHHRKENQTETSGFE 159
           MT+V+C MPS DD D+PAKCRERRRRRIEMRRLATV SANPS ST+HH+KENQT TSGFE
Sbjct: 1   MTEVHCSMPSGDDEDTPAKCRERRRRRIEMRRLATVSSANPSPSTAHHQKENQTGTSGFE 60

Query: 160 KKRVRKTDGDGTPEISSSSSSGEDVKTVVASPSVPQPVFGMMSVSGRSREMEDAVSVSTW 219
           KKR +KTDGDG PEISSSSSSGEDVKTV ASPSVPQPVFGMMSVSGRSREMEDAV VST 
Sbjct: 61  KKRGQKTDGDGNPEISSSSSSGEDVKTVRASPSVPQPVFGMMSVSGRSREMEDAVCVSTC 120

Query: 220 LLGPENCRRLPVHFFAVYDGHGGPHVAALCREKMHVLVREEFSRVVGTRQETESGGGGSS 279
           +LG EN RR  VHFFAVYDGHGGPHVAALCREKMHV V+EEFSRV+ TR E ESGGGGSS
Sbjct: 121 VLGSENFRRQVVHFFAVYDGHGGPHVAALCREKMHVFVQEEFSRVISTRGENESGGGGSS 180

Query: 280 TADEVKVEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL 339
             +EVK EEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL
Sbjct: 181 AGEEVKFEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL 240

Query: 340 TPDHIIVANCGDSRAVLCRGGRAIPLSNDHKPDRIDELARIEAAGGRVIFVNGARVEGIL 399
           TPDHIIVANCGDSRAVLCRGG AIPLS DHKPDR DELARIEAAGGRVIFVNGARVEGIL
Sbjct: 241 TPDHIIVANCGDSRAVLCRGGTAIPLSIDHKPDRNDELARIEAAGGRVIFVNGARVEGIL 300

Query: 400 AMSRAIGQKY---EVISERMHDVAFTHQQ 426
           AMSRAIG KY    VISE   +V FT ++
Sbjct: 301 AMSRAIGDKYLKSVVISE--PEVTFTKRE 327

BLAST of Lag0019953.1 vs. ExPASy Swiss-Prot
Match: Q9FLI3 (Probable protein phosphatase 2C 75 OS=Arabidopsis thaliana OX=3702 GN=AHG1 PE=1 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 2.2e-73
Identity = 171/323 (52.94%), Postives = 208/323 (64.40%), Query Frame = 0

Query: 100 MTDVYCRMPSEDDGD--SPAKCRERRRRRIEMRRLATVPSANPSSSTSHHRKENQTETSG 159
           MT++Y R  S   GD  SP KCRERRRRRIEMRR A V    PSSS +  R + +  +S 
Sbjct: 1   MTEIY-RTISTGRGDDVSPTKCRERRRRRIEMRRQAAV-FGEPSSSRNRDRTDMEVYSS- 60

Query: 160 FE---KKRVRKTDGDGTP--------EISSSSSSGEDVKTVVASPSVPQPVFGMMSVSGR 219
           F+   +K+ R+++  G P          ++SS    +        +  +P++G++SV GR
Sbjct: 61  FDVPLRKQARRSEIGGLPADIGGFLAPPAASSCQKSEAPVWKGEETEDEPLYGIVSVMGR 120

Query: 220 SREMEDAVSVSTWLLGPENCRRLPVHFFAVYDGHGGPHVAALCREKMHVLVREEFSRVVG 279
           SR+MED+V+V   L  PE  R+ PVHFFAVYDGHGG  V+ LC   MH  V+EE  +   
Sbjct: 121 SRKMEDSVTVKPNLCKPEVNRQRPVHFFAVYDGHGGSQVSTLCSTTMHTFVKEELEQ--- 180

Query: 280 TRQETESGGGGSSTADEVKVEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPME 339
             +E E G    S  D V    E  WR VM+RSF+RMDE+A STC CG+    C C P E
Sbjct: 181 NLEEEEEG----SENDVV----ERKWRGVMKRSFKRMDEMATSTCVCGTSVPLCNCDPRE 240

Query: 340 VALGGSTAVVAVLTPDHIIVANCGDSRAVLCRGGRAIPLSNDHKPDRIDELARIEAAGGR 399
            A+ GSTAV AVLT DHIIVAN GDSRAVLCR G AIPLSNDHKPDR DE ARIEAAGGR
Sbjct: 241 AAISGSTAVTAVLTHDHIIVANTGDSRAVLCRNGMAIPLSNDHKPDRPDERARIEAAGGR 300

Query: 400 VIFVNGARVEGILAMSRAIGQKY 410
           V+ V+GARVEGILA SRAIG +Y
Sbjct: 301 VLVVDGARVEGILATSRAIGDRY 309

BLAST of Lag0019953.1 vs. ExPASy Swiss-Prot
Match: Q84JI0 (Probable protein phosphatase 2C 30 OS=Oryza sativa subsp. japonica OX=39947 GN=PP2C30 PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.5e-48
Identity = 139/320 (43.44%), Postives = 169/320 (52.81%), Query Frame = 0

Query: 100 MTDVYCRM--PSEDDGDSP---AKCRERRRRRIEMRRLATVPSANPSSSTSHHRKENQTE 159
           M ++ C +   S  +G  P      R  RRRR+E+RRL  V        TS  R      
Sbjct: 1   MAEICCEVVAGSSSEGKGPECDTGSRAARRRRMEIRRLRVVAERGAEEETSGKR------ 60

Query: 160 TSGFEKKRVRKTDGDGTPEISSSSSSGEDVKTVVASPSVPQPVFGMMSVSGRSREMEDAV 219
                    R+ DG G      +S+  ED         V +  +G  SV GR R+MED+V
Sbjct: 61  ---------RRLDGGG----GEASTDEED-------REVERARYGFTSVCGRRRDMEDSV 120

Query: 220 SVSTWLLGPENCRRLPVHFFAVYDGHGGPHVAALCREKMHVLVREEFSRVVGTRQETESG 279
           S     L          HFF V+DGHG  HVA  C ++MH +V +E              
Sbjct: 121 SACPGFLPGH-------HFFGVFDGHGCSHVATSCGQRMHEIVVDE-------------- 180

Query: 280 GGGSSTADEVKVEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALG---- 339
               + A    ++EEA WR VM RSF RMD  A+++ + GSV     C   E+ L     
Sbjct: 181 --AGAAAGSAGLDEEARWRGVMERSFARMDAEAVAS-SRGSVAPAPTCR-CEMQLPKCDH 240

Query: 340 -GSTAVVAVLTPDHIIVANCGDSRAVLCRGGRAIPLSNDHKPDRIDELARIEAAGGRVIF 399
            GSTAVVAVL P H++VANCGDSRAVLCRGG AIPLS DHKPDR DEL RI AAGGRVIF
Sbjct: 241 VGSTAVVAVLGPRHVVVANCGDSRAVLCRGGAAIPLSCDHKPDRPDELERIHAAGGRVIF 269

Query: 400 VNGARVEGILAMSRAIGQKY 410
            +GARV G+LAMSRAIG  Y
Sbjct: 301 WDGARVFGMLAMSRAIGDSY 269

BLAST of Lag0019953.1 vs. ExPASy Swiss-Prot
Match: Q9ZW21 (Probable protein phosphatase 2C 24 OS=Arabidopsis thaliana OX=3702 GN=At2g29380 PE=1 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 3.2e-48
Identity = 127/280 (45.36%), Postives = 158/280 (56.43%), Query Frame = 0

Query: 136 PSANPSSSTSHHRKENQTET---SGFEKKRVR-KTDGDGTPEISSSSSSGEDVKTVVASP 195
           PS+   S+ +H R+  + +T     +EK   R K +   T   S   SS ED      + 
Sbjct: 14  PSSVYESTPAHSRRRPRFQTVMHEDWEKNCKRSKQEALATRYSSIPRSSREDFSD--QNV 73

Query: 196 SVPQPVFGMMSVSGRSREMEDAVSVSTWLLGPENCRRLPVHFFAVYDGHGGPHVAALCRE 255
            V  P +G+ SV GR REMEDAV++      P+N    P H+F VYDGHG  HVAA CRE
Sbjct: 74  DVSSPRYGVSSVCGRRREMEDAVAIHPSFSSPKN-SEFPQHYFGVYDGHGCSHVAARCRE 133

Query: 256 KMHVLVREEFSRVVGTRQETESGGGGSSTADEVKVEEEATWRRVMRRSFERMDEVALSTC 315
           ++H LV+EE S                       +E+E  W+  M RSF RMD+  +S  
Sbjct: 134 RLHKLVQEELSS---------------------DMEDEEEWKTTMERSFTRMDKEVVS-W 193

Query: 316 ACGSVGGQCGCHPMEVALG--GSTAVVAVLTPDHIIVANCGDSRAVLCRGGRAIPLSNDH 375
               V   C C     A    GSTAVV+V+TPD I+VANCGDSRAVLCR G+ +PLS DH
Sbjct: 194 GDSVVTANCKCDLQTPACDSVGSTAVVSVITPDKIVVANCGDSRAVLCRNGKPVPLSTDH 253

Query: 376 KPDRIDELARIEAAGGRVIFVNGARVEGILAMSRAIGQKY 410
           KPDR DEL RIE AGGRVI+ +  RV G+LAMSRAIG  Y
Sbjct: 254 KPDRPDELDRIEGAGGRVIYWDCPRVLGVLAMSRAIGDNY 268

BLAST of Lag0019953.1 vs. ExPASy Swiss-Prot
Match: Q0J2L7 (Probable protein phosphatase 2C 68 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0325700 PE=2 SV=2)

HSP 1 Score: 190.3 bits (482), Expect = 4.7e-47
Identity = 116/234 (49.57%), Postives = 133/234 (56.84%), Query Frame = 0

Query: 178 SSSGEDVKTVVASPSVPQPVFGMMSVSGRSREMEDAVSVSTWLLGPEN--CRRLPVHFFA 237
           +S GE    VVA         G  SV+GR REMEDAVS+      P N         F+ 
Sbjct: 61  ASGGEAGLVVVAKR------HGAASVAGRRREMEDAVSLREAFAAPANGEVAAARCDFYG 120

Query: 238 VYDGHGGPHVAALCREKMHVLVREEFSRVVGTRQETESGGGGSSTADEVKVEEEATWRRV 297
           V+DGHG  HVA  CRE+MH LV EE              G GS  A      E A+W   
Sbjct: 121 VFDGHGCSHVADACRERMHELVAEEM-------------GAGSPAA---AAREPASWTET 180

Query: 298 MRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVLTPDHIIVANCGDSRAV 357
           M RSF RMD   ++ C   S  G C C   +    GSTAVVAV+    ++VANCGDSRAV
Sbjct: 181 MERSFARMDAEVIAGCRAES--GSCRCEGQKCDHVGSTAVVAVVEESRVVVANCGDSRAV 240

Query: 358 LCRGGRAIPLSNDHKPDRIDELARIEAAGGRVIFVNGARVEGILAMSRAIGQKY 410
           LCRGG  + LS+DHKPDR DEL RIEAAGGRVIF  GARV G+LAMSR+IG  Y
Sbjct: 241 LCRGGAPVQLSSDHKPDRPDELERIEAAGGRVIFWEGARVLGVLAMSRSIGDAY 270

BLAST of Lag0019953.1 vs. ExPASy Swiss-Prot
Match: Q9LNW3 (Protein phosphatase 2C 3 OS=Arabidopsis thaliana OX=3702 GN=AIP1 PE=1 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.8e-46
Identity = 136/329 (41.34%), Postives = 179/329 (54.41%), Query Frame = 0

Query: 114 DSPAKCRER-----RRRRIEMRRLATVPS-ANPSSSTSH--------HRKENQTETSGF- 173
           D  + C  R     R+ RI ++R    PS  NP++ST+         + K N+ E   F 
Sbjct: 8   DETSACESRPLWSSRKWRIGVQRFRMSPSEMNPTASTTEEEDKSEGIYNKRNKQEEYDFM 67

Query: 174 ------------EKKRVRKTDGDGTPEISSSSSSGEDVKTVVASPSVPQ----PVFGMMS 233
                       E++ V   D D    IS  +SS  DV  + +  +V +    P +G+ S
Sbjct: 68  NCASSSPSQSSPEEESVSLEDSD--VSISDGNSSVNDVAVIPSKKTVKETDLRPRYGVAS 127

Query: 234 VSGRSREMEDAVSV-STWLLGPENCRRLPVHFFAVYDGHGGPHVAALCREKMHVLVREEF 293
           V GR R+MEDAV++  +++       R   H+F VYDGHG  HVAA C+E++H LV+EE 
Sbjct: 128 VCGRRRDMEDAVALHPSFVRKQTEFSRTRWHYFGVYDGHGCSHVAARCKERLHELVQEE- 187

Query: 294 SRVVGTRQETESGGGGSSTADEVKVEEEATWRRVMRRSFERMD-EVALSTCACGSVGGQC 353
                              A   K EE   W+++M RSF RMD EV        S   +C
Sbjct: 188 -------------------ALSDKKEE---WKKMMERSFTRMDKEVVRWGETVMSANCRC 247

Query: 354 GCHPMEVALGGSTAVVAVLTPDHIIVANCGDSRAVLCRGGRAIPLSNDHKPDRIDELARI 410
                +    GSTAVV+V+TP+ IIVANCGDSRAVLCR G+A+PLS DHKPDR DEL RI
Sbjct: 248 ELQTPDCDAVGSTAVVSVITPEKIIVANCGDSRAVLCRNGKAVPLSTDHKPDRPDELDRI 307

BLAST of Lag0019953.1 vs. ExPASy TrEMBL
Match: A0A1S3AYN8 (Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103484021 PE=3 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 2.8e-151
Identity = 283/329 (86.02%), Postives = 298/329 (90.58%), Query Frame = 0

Query: 100 MTDVYCRMPSEDDGDSPAKCRERRRRRIEMRRLATVPSANPSSSTSHHRKENQTETSGFE 159
           MT+V+CRMPS DD DSPAKCRERRRRRIEMRRLATV SANPS ST+HHRKENQT TSGFE
Sbjct: 1   MTEVHCRMPSGDDEDSPAKCRERRRRRIEMRRLATVSSANPSPSTAHHRKENQTGTSGFE 60

Query: 160 KKRVRKTDGDGTPEISSSSSSGEDVKTVVASPSVPQPVFGMMSVSGRSREMEDAVSVSTW 219
           KKR +KT+GDG PEISSSSSSGEDVKTV ASPSVPQPVFGMMSVSGRSREMEDAVSVST+
Sbjct: 61  KKRGQKTEGDGNPEISSSSSSGEDVKTVKASPSVPQPVFGMMSVSGRSREMEDAVSVSTF 120

Query: 220 LLGPENCRRLPVHFFAVYDGHGGPHVAALCREKMHVLVREEFSRVVGTRQETESGGGGSS 279
           ++G EN RR  VHFFAVYDGHGGPHVAALCREKMHV V+EEFSRV+ TR+E ESGGGGSS
Sbjct: 121 VVGSENFRRQVVHFFAVYDGHGGPHVAALCREKMHVFVQEEFSRVISTREENESGGGGSS 180

Query: 280 TADEVKVEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL 339
             +EV  EEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL
Sbjct: 181 AGEEVNFEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL 240

Query: 340 TPDHIIVANCGDSRAVLCRGGRAIPLSNDHKPDRIDELARIEAAGGRVIFVNGARVEGIL 399
           TPDHIIVANCGDSRAVLCRGGRAIPLS DHKPDR DELARIEAAGGRVIFVNGARVEGIL
Sbjct: 241 TPDHIIVANCGDSRAVLCRGGRAIPLSIDHKPDRNDELARIEAAGGRVIFVNGARVEGIL 300

Query: 400 AMSRAIGQKY---EVISERMHDVAFTHQQ 426
           AMSRAIG KY    VISE   +V FT ++
Sbjct: 301 AMSRAIGDKYLKSVVISE--PEVTFTKRE 327

BLAST of Lag0019953.1 vs. ExPASy TrEMBL
Match: A0A1S3AYP6 (Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103484021 PE=3 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 8.9e-150
Identity = 275/309 (89.00%), Postives = 287/309 (92.88%), Query Frame = 0

Query: 100 MTDVYCRMPSEDDGDSPAKCRERRRRRIEMRRLATVPSANPSSSTSHHRKENQTETSGFE 159
           MT+V+CRMPS DD DSPAKCRERRRRRIEMRRLATV SANPS ST+HHRKENQT TSGFE
Sbjct: 1   MTEVHCRMPSGDDEDSPAKCRERRRRRIEMRRLATVSSANPSPSTAHHRKENQTGTSGFE 60

Query: 160 KKRVRKTDGDGTPEISSSSSSGEDVKTVVASPSVPQPVFGMMSVSGRSREMEDAVSVSTW 219
           KKR +KT+GDG PEISSSSSSGEDVKTV ASPSVPQPVFGMMSVSGRSREMEDAVSVST+
Sbjct: 61  KKRGQKTEGDGNPEISSSSSSGEDVKTVKASPSVPQPVFGMMSVSGRSREMEDAVSVSTF 120

Query: 220 LLGPENCRRLPVHFFAVYDGHGGPHVAALCREKMHVLVREEFSRVVGTRQETESGGGGSS 279
           ++G EN RR  VHFFAVYDGHGGPHVAALCREKMHV V+EEFSRV+ TR+E ESGGGGSS
Sbjct: 121 VVGSENFRRQVVHFFAVYDGHGGPHVAALCREKMHVFVQEEFSRVISTREENESGGGGSS 180

Query: 280 TADEVKVEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL 339
             +EV  EEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL
Sbjct: 181 AGEEVNFEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL 240

Query: 340 TPDHIIVANCGDSRAVLCRGGRAIPLSNDHKPDRIDELARIEAAGGRVIFVNGARVEGIL 399
           TPDHIIVANCGDSRAVLCRGGRAIPLS DHKPDR DELARIEAAGGRVIFVNGARVEGIL
Sbjct: 241 TPDHIIVANCGDSRAVLCRGGRAIPLSIDHKPDRNDELARIEAAGGRVIFVNGARVEGIL 300

Query: 400 AMSRAIGQK 409
           AMSRAIG K
Sbjct: 301 AMSRAIGMK 309

BLAST of Lag0019953.1 vs. ExPASy TrEMBL
Match: A0A0A0L8I0 (Protein-serine/threonine phosphatase OS=Cucumis sativus OX=3659 GN=Csa_3G178550 PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 5.8e-149
Identity = 281/329 (85.41%), Postives = 294/329 (89.36%), Query Frame = 0

Query: 100 MTDVYCRMPSEDDGDSPAKCRERRRRRIEMRRLATVPSANPSSSTSHHRKENQTETSGFE 159
           MT+V+C MPS DD D+PAKCRERRRRRIEMRRLATV SANPS ST+HH+KENQT TSGFE
Sbjct: 1   MTEVHCSMPSGDDEDTPAKCRERRRRRIEMRRLATVSSANPSPSTAHHQKENQTGTSGFE 60

Query: 160 KKRVRKTDGDGTPEISSSSSSGEDVKTVVASPSVPQPVFGMMSVSGRSREMEDAVSVSTW 219
           KKR +KTDGDG PEISSSSSSGEDVKTV ASPSVPQPVFGMMSVSGRSREMEDAV VST 
Sbjct: 61  KKRGQKTDGDGNPEISSSSSSGEDVKTVRASPSVPQPVFGMMSVSGRSREMEDAVCVSTC 120

Query: 220 LLGPENCRRLPVHFFAVYDGHGGPHVAALCREKMHVLVREEFSRVVGTRQETESGGGGSS 279
           +LG EN RR  VHFFAVYDGHGGPHVAALCREKMHV V+EEFSRV+ TR E ESGGGGSS
Sbjct: 121 VLGSENFRRQVVHFFAVYDGHGGPHVAALCREKMHVFVQEEFSRVISTRGENESGGGGSS 180

Query: 280 TADEVKVEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL 339
             +EVK EEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL
Sbjct: 181 AGEEVKFEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL 240

Query: 340 TPDHIIVANCGDSRAVLCRGGRAIPLSNDHKPDRIDELARIEAAGGRVIFVNGARVEGIL 399
           TPDHIIVANCGDSRAVLCRGG AIPLS DHKPDR DELARIEAAGGRVIFVNGARVEGIL
Sbjct: 241 TPDHIIVANCGDSRAVLCRGGTAIPLSIDHKPDRNDELARIEAAGGRVIFVNGARVEGIL 300

Query: 400 AMSRAIGQKY---EVISERMHDVAFTHQQ 426
           AMSRAIG KY    VISE   +V FT ++
Sbjct: 301 AMSRAIGDKYLKSVVISE--PEVTFTKRE 327

BLAST of Lag0019953.1 vs. ExPASy TrEMBL
Match: A0A5A7SWG2 (Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold440G00050 PE=3 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 7.5e-149
Identity = 274/306 (89.54%), Postives = 285/306 (93.14%), Query Frame = 0

Query: 100 MTDVYCRMPSEDDGDSPAKCRERRRRRIEMRRLATVPSANPSSSTSHHRKENQTETSGFE 159
           MT+V+CRMPS DD DSPAKCRERRRRRIEMRRLATV SANPS ST+HHRKENQT TSGFE
Sbjct: 1   MTEVHCRMPSGDDEDSPAKCRERRRRRIEMRRLATVSSANPSPSTAHHRKENQTGTSGFE 60

Query: 160 KKRVRKTDGDGTPEISSSSSSGEDVKTVVASPSVPQPVFGMMSVSGRSREMEDAVSVSTW 219
           KKR +KTDGDG PEISSSSSSGEDVKTV ASPSVPQPVFGMMSVSGRSREMEDAVSVST+
Sbjct: 61  KKRGQKTDGDGNPEISSSSSSGEDVKTVKASPSVPQPVFGMMSVSGRSREMEDAVSVSTF 120

Query: 220 LLGPENCRRLPVHFFAVYDGHGGPHVAALCREKMHVLVREEFSRVVGTRQETESGGGGSS 279
           ++G EN RR  VHFFAVYDGHGGPHVAALCREKMHV V+EEFSRV+ TR+E ESGGGGSS
Sbjct: 121 VVGSENFRRQVVHFFAVYDGHGGPHVAALCREKMHVFVQEEFSRVISTREENESGGGGSS 180

Query: 280 TADEVKVEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL 339
             +EV  EEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL
Sbjct: 181 AGEEVNFEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVL 240

Query: 340 TPDHIIVANCGDSRAVLCRGGRAIPLSNDHKPDRIDELARIEAAGGRVIFVNGARVEGIL 399
           TPDHIIVANCGDSRAVLCRGGRAIPLS DHKPDR DELARIEAAGGRVIFVNGARVEGIL
Sbjct: 241 TPDHIIVANCGDSRAVLCRGGRAIPLSIDHKPDRNDELARIEAAGGRVIFVNGARVEGIL 300

Query: 400 AMSRAI 406
           AMSRAI
Sbjct: 301 AMSRAI 306

BLAST of Lag0019953.1 vs. ExPASy TrEMBL
Match: A0A6J1CJV4 (Protein-serine/threonine phosphatase OS=Momordica charantia OX=3673 GN=LOC111011675 PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 1.3e-148
Identity = 307/432 (71.06%), Postives = 320/432 (74.07%), Query Frame = 0

Query: 12  RSGGAGFAAARRIRIAREPLF-FPARR--------RTGSEKGKNNLIFPTEENTQRHVF- 71
           R  G    AAR IR+AREPL  F ARR          GSE+ +NN+IF   EN       
Sbjct: 6   RRVGQVVLAARGIRVAREPLLCFSARRGGEVGEGKENGSER-ENNIIFLRRENDDEFPSE 65

Query: 72  -FSAKFRTAFHYFLILHFADDDCPHREILVGLFLLRTIPMTDVYCRMPSEDDGDSPAKCR 131
             SA  R     + I  FADDD P R+IL G FLLR +PMT   CRMPSED+GDSPAKCR
Sbjct: 66  PNSAPHRIPLLGYNIHFFADDDSPQRDILDGEFLLRMVPMTAACCRMPSEDEGDSPAKCR 125

Query: 132 ERRRRRIEMRRLATVPSANPSSSTSHHRKENQTETSGFEKKRVRKTDGDGTPEISSSSSS 191
           ERRRRRIEMRRLAT  SANPS STSHHRKEN       EKKRVRKTDG  TPEISSSSSS
Sbjct: 126 ERRRRRIEMRRLATTSSANPSPSTSHHRKENNQT----EKKRVRKTDGSATPEISSSSSS 185

Query: 192 GEDVKTVVASPSVPQPVFGMMSVSGRSREMEDAVSVSTWLLGPENCRRLPVHFFAVYDGH 251
           GEDVKT VASPSVPQPVFGMMSVSGRSREMEDAVS ST LLGPENCRR PVHFFAVYDGH
Sbjct: 186 GEDVKTAVASPSVPQPVFGMMSVSGRSREMEDAVSASTCLLGPENCRRRPVHFFAVYDGH 245

Query: 252 GGPHVAALCREKMHVLVREEFSRVVGTRQETESGGGGSST-------ADEVKVEEEATWR 311
           GGPHVAALCREKMHVLV+EE +RVVGTR E ESGGG SS                     
Sbjct: 246 GGPHVAALCREKMHVLVQEELARVVGTRDEAESGGGSSSAXXXXXXXXXXXXXXXXXXXX 305

Query: 312 RVMRRSFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVLTPDHIIVANCGDSR 371
                     DEVALSTCACGSVGGQCGCHPMEVALGGSTAVVA LTP+HI+VANCGDSR
Sbjct: 306 XXXXXXXXXXDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAALTPNHIVVANCGDSR 365

Query: 372 AVLCRGGRAIPLSNDHKPDRIDELARIEAAGGRVIFVNGARVEGILAMSRAIGQKY---E 423
           AVLCRGGRAIPLSNDHKPDRIDELARIEAAGGRVIFVNGARVEGILAMSRAIG KY    
Sbjct: 366 AVLCRGGRAIPLSNDHKPDRIDELARIEAAGGRVIFVNGARVEGILAMSRAIGDKYLKSV 425

BLAST of Lag0019953.1 vs. TAIR 10
Match: AT5G51760.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.6e-74
Identity = 171/323 (52.94%), Postives = 208/323 (64.40%), Query Frame = 0

Query: 100 MTDVYCRMPSEDDGD--SPAKCRERRRRRIEMRRLATVPSANPSSSTSHHRKENQTETSG 159
           MT++Y R  S   GD  SP KCRERRRRRIEMRR A V    PSSS +  R + +  +S 
Sbjct: 1   MTEIY-RTISTGRGDDVSPTKCRERRRRRIEMRRQAAV-FGEPSSSRNRDRTDMEVYSS- 60

Query: 160 FE---KKRVRKTDGDGTP--------EISSSSSSGEDVKTVVASPSVPQPVFGMMSVSGR 219
           F+   +K+ R+++  G P          ++SS    +        +  +P++G++SV GR
Sbjct: 61  FDVPLRKQARRSEIGGLPADIGGFLAPPAASSCQKSEAPVWKGEETEDEPLYGIVSVMGR 120

Query: 220 SREMEDAVSVSTWLLGPENCRRLPVHFFAVYDGHGGPHVAALCREKMHVLVREEFSRVVG 279
           SR+MED+V+V   L  PE  R+ PVHFFAVYDGHGG  V+ LC   MH  V+EE  +   
Sbjct: 121 SRKMEDSVTVKPNLCKPEVNRQRPVHFFAVYDGHGGSQVSTLCSTTMHTFVKEELEQ--- 180

Query: 280 TRQETESGGGGSSTADEVKVEEEATWRRVMRRSFERMDEVALSTCACGSVGGQCGCHPME 339
             +E E G    S  D V    E  WR VM+RSF+RMDE+A STC CG+    C C P E
Sbjct: 181 NLEEEEEG----SENDVV----ERKWRGVMKRSFKRMDEMATSTCVCGTSVPLCNCDPRE 240

Query: 340 VALGGSTAVVAVLTPDHIIVANCGDSRAVLCRGGRAIPLSNDHKPDRIDELARIEAAGGR 399
            A+ GSTAV AVLT DHIIVAN GDSRAVLCR G AIPLSNDHKPDR DE ARIEAAGGR
Sbjct: 241 AAISGSTAVTAVLTHDHIIVANTGDSRAVLCRNGMAIPLSNDHKPDRPDERARIEAAGGR 300

Query: 400 VIFVNGARVEGILAMSRAIGQKY 410
           V+ V+GARVEGILA SRAIG +Y
Sbjct: 301 VLVVDGARVEGILATSRAIGDRY 309

BLAST of Lag0019953.1 vs. TAIR 10
Match: AT2G29380.1 (highly ABA-induced PP2C gene 3 )

HSP 1 Score: 194.1 bits (492), Expect = 2.3e-49
Identity = 127/280 (45.36%), Postives = 158/280 (56.43%), Query Frame = 0

Query: 136 PSANPSSSTSHHRKENQTET---SGFEKKRVR-KTDGDGTPEISSSSSSGEDVKTVVASP 195
           PS+   S+ +H R+  + +T     +EK   R K +   T   S   SS ED      + 
Sbjct: 14  PSSVYESTPAHSRRRPRFQTVMHEDWEKNCKRSKQEALATRYSSIPRSSREDFSD--QNV 73

Query: 196 SVPQPVFGMMSVSGRSREMEDAVSVSTWLLGPENCRRLPVHFFAVYDGHGGPHVAALCRE 255
            V  P +G+ SV GR REMEDAV++      P+N    P H+F VYDGHG  HVAA CRE
Sbjct: 74  DVSSPRYGVSSVCGRRREMEDAVAIHPSFSSPKN-SEFPQHYFGVYDGHGCSHVAARCRE 133

Query: 256 KMHVLVREEFSRVVGTRQETESGGGGSSTADEVKVEEEATWRRVMRRSFERMDEVALSTC 315
           ++H LV+EE S                       +E+E  W+  M RSF RMD+  +S  
Sbjct: 134 RLHKLVQEELSS---------------------DMEDEEEWKTTMERSFTRMDKEVVS-W 193

Query: 316 ACGSVGGQCGCHPMEVALG--GSTAVVAVLTPDHIIVANCGDSRAVLCRGGRAIPLSNDH 375
               V   C C     A    GSTAVV+V+TPD I+VANCGDSRAVLCR G+ +PLS DH
Sbjct: 194 GDSVVTANCKCDLQTPACDSVGSTAVVSVITPDKIVVANCGDSRAVLCRNGKPVPLSTDH 253

Query: 376 KPDRIDELARIEAAGGRVIFVNGARVEGILAMSRAIGQKY 410
           KPDR DEL RIE AGGRVI+ +  RV G+LAMSRAIG  Y
Sbjct: 254 KPDRPDELDRIEGAGGRVIYWDCPRVLGVLAMSRAIGDNY 268

BLAST of Lag0019953.1 vs. TAIR 10
Match: AT1G07430.1 (highly ABA-induced PP2C gene 2 )

HSP 1 Score: 188.3 bits (477), Expect = 1.3e-47
Identity = 136/329 (41.34%), Postives = 179/329 (54.41%), Query Frame = 0

Query: 114 DSPAKCRER-----RRRRIEMRRLATVPS-ANPSSSTSH--------HRKENQTETSGF- 173
           D  + C  R     R+ RI ++R    PS  NP++ST+         + K N+ E   F 
Sbjct: 8   DETSACESRPLWSSRKWRIGVQRFRMSPSEMNPTASTTEEEDKSEGIYNKRNKQEEYDFM 67

Query: 174 ------------EKKRVRKTDGDGTPEISSSSSSGEDVKTVVASPSVPQ----PVFGMMS 233
                       E++ V   D D    IS  +SS  DV  + +  +V +    P +G+ S
Sbjct: 68  NCASSSPSQSSPEEESVSLEDSD--VSISDGNSSVNDVAVIPSKKTVKETDLRPRYGVAS 127

Query: 234 VSGRSREMEDAVSV-STWLLGPENCRRLPVHFFAVYDGHGGPHVAALCREKMHVLVREEF 293
           V GR R+MEDAV++  +++       R   H+F VYDGHG  HVAA C+E++H LV+EE 
Sbjct: 128 VCGRRRDMEDAVALHPSFVRKQTEFSRTRWHYFGVYDGHGCSHVAARCKERLHELVQEE- 187

Query: 294 SRVVGTRQETESGGGGSSTADEVKVEEEATWRRVMRRSFERMD-EVALSTCACGSVGGQC 353
                              A   K EE   W+++M RSF RMD EV        S   +C
Sbjct: 188 -------------------ALSDKKEE---WKKMMERSFTRMDKEVVRWGETVMSANCRC 247

Query: 354 GCHPMEVALGGSTAVVAVLTPDHIIVANCGDSRAVLCRGGRAIPLSNDHKPDRIDELARI 410
                +    GSTAVV+V+TP+ IIVANCGDSRAVLCR G+A+PLS DHKPDR DEL RI
Sbjct: 248 ELQTPDCDAVGSTAVVSVITPEKIIVANCGDSRAVLCRNGKAVPLSTDHKPDRPDELDRI 307

BLAST of Lag0019953.1 vs. TAIR 10
Match: AT5G59220.1 (highly ABA-induced PP2C gene 1 )

HSP 1 Score: 184.9 bits (468), Expect = 1.4e-46
Identity = 127/291 (43.64%), Postives = 155/291 (53.26%), Query Frame = 0

Query: 123 RRRRIEMRRLATVPSANPSSSTSH--HRKENQTETSGFEKKRVRKTDGDGTPEISSSSSS 182
           RRRR+E+RR   V              R+  +  T       V          +  S SS
Sbjct: 39  RRRRMEIRRFKFVSGEQEPVFVDGDLQRRRRRESTVAASTSTVFYETAKEVVVLCESLSS 98

Query: 183 GEDVKTVVASPS-VPQPVFGMMSVSGRSREMEDAVSVSTWLLGPE-NCRRLPVHFFAVYD 242
                TVVA P     P +G+ SV GR REMEDAV+V  +    +        H+  VYD
Sbjct: 99  -----TVVALPDPEAYPKYGVASVCGRRREMEDAVAVHPFFSRHQTEYSSTGFHYCGVYD 158

Query: 243 GHGGPHVAALCREKMHVLVREEFSRVVGTRQETESGGGGSSTADEVKVEEEATWRRVMRR 302
           GHG  HVA  CRE++H LVREEF                         E +A W + M R
Sbjct: 159 GHGCSHVAMKCRERLHELVREEF-------------------------EADADWEKSMAR 218

Query: 303 SFERMDEVALSTCACGSVGGQCGCHPMEVALGGSTAVVAVLTPDHIIVANCGDSRAVLCR 362
           SF RMD   ++  A G+   +C     +    GSTAVV+VLTP+ IIVANCGDSRAVLCR
Sbjct: 219 SFTRMDMEVVALNADGAAKCRCELQRPDCDAVGSTAVVSVLTPEKIIVANCGDSRAVLCR 278

Query: 363 GGRAIPLSNDHKPDRIDELARIEAAGGRVIFVNGARVEGILAMSRAIGQKY 410
            G+AI LS+DHKPDR DEL RI+AAGGRVI+ +G RV G+LAMSRAIG  Y
Sbjct: 279 NGKAIALSSDHKPDRPDELDRIQAAGGRVIYWDGPRVLGVLAMSRAIGDNY 299

BLAST of Lag0019953.1 vs. TAIR 10
Match: AT4G26080.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 167.9 bits (424), Expect = 1.8e-41
Identity = 103/222 (46.40%), Postives = 126/222 (56.76%), Query Frame = 0

Query: 196 PVFGMMSVSGRSREMEDAVSVSTWLLGPENCRRL--------PVHFFAVYDGHGGPHVAA 255
           P++G  S+ GR  EMEDAVS     L   +   L          HFF VYDGHGG  VA 
Sbjct: 127 PLYGFTSICGRRPEMEDAVSTIPRFLQSSSGSMLDGRFDPQSAAHFFGVYDGHGGSQVAN 186

Query: 256 LCREKMHVLVREEFSRVVGTRQETESGGGGSSTADEVKVEEEATWRRVMRRSFERMDEVA 315
            CRE+MH+ + EE ++      E      G +  ++        W++ +  SF R+D   
Sbjct: 187 YCRERMHLALAEEIAK------EKPMLCDGDTWLEK--------WKKALFNSFLRVDSEI 246

Query: 316 LSTCACGSVGGQCGCHPMEVALGGSTAVVAVLTPDHIIVANCGDSRAVLCRGGRAIPLSN 375
            S              P  V   GST+VVAV+ P HI VANCGDSRAVLCRG  A+PLS 
Sbjct: 247 ESVA------------PETV---GSTSVVAVVFPSHIFVANCGDSRAVLCRGKTALPLSV 306

Query: 376 DHKPDRIDELARIEAAGGRVIFVNGARVEGILAMSRAIGQKY 410
           DHKPDR DE ARIEAAGG+VI  NGARV G+LAMSR+IG +Y
Sbjct: 307 DHKPDREDEAARIEAAGGKVIQWNGARVFGVLAMSRSIGDRY 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7013190.15.0e-15573.62Subtilisin-like protease SBT1.3, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_008439132.15.7e-15186.02PREDICTED: probable protein phosphatase 2C 75 isoform X1 [Cucumis melo][more]
XP_008439133.11.8e-14989.00PREDICTED: probable protein phosphatase 2C 75 isoform X2 [Cucumis melo][more]
XP_038883683.13.1e-14985.80probable protein phosphatase 2C 75 isoform X1 [Benincasa hispida][more]
XP_004148120.21.2e-14885.41probable protein phosphatase 2C 75 [Cucumis sativus] >KGN57314.1 hypothetical pr... [more]
Match NameE-valueIdentityDescription
Q9FLI32.2e-7352.94Probable protein phosphatase 2C 75 OS=Arabidopsis thaliana OX=3702 GN=AHG1 PE=1 ... [more]
Q84JI02.5e-4843.44Probable protein phosphatase 2C 30 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Q9ZW213.2e-4845.36Probable protein phosphatase 2C 24 OS=Arabidopsis thaliana OX=3702 GN=At2g29380 ... [more]
Q0J2L74.7e-4749.57Probable protein phosphatase 2C 68 OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
Q9LNW31.8e-4641.34Protein phosphatase 2C 3 OS=Arabidopsis thaliana OX=3702 GN=AIP1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3AYN82.8e-15186.02Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103484021 PE=... [more]
A0A1S3AYP68.9e-15089.00Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103484021 PE=... [more]
A0A0A0L8I05.8e-14985.41Protein-serine/threonine phosphatase OS=Cucumis sativus OX=3659 GN=Csa_3G178550 ... [more]
A0A5A7SWG27.5e-14989.54Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A6J1CJV41.3e-14871.06Protein-serine/threonine phosphatase OS=Momordica charantia OX=3673 GN=LOC111011... [more]
Match NameE-valueIdentityDescription
AT5G51760.11.6e-7452.94Protein phosphatase 2C family protein [more]
AT2G29380.12.3e-4945.36highly ABA-induced PP2C gene 3 [more]
AT1G07430.11.3e-4741.34highly ABA-induced PP2C gene 2 [more]
AT5G59220.11.4e-4643.64highly ABA-induced PP2C gene 1 [more]
AT4G26080.11.8e-4146.40Protein phosphatase 2C family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001932PPM-type phosphatase domainSMARTSM00332PP2C_4coord: 187..434
e-value: 1.6E-41
score: 153.9
IPR001932PPM-type phosphatase domainPFAMPF00481PP2Ccoord: 198..409
e-value: 1.2E-37
score: 130.0
IPR001932PPM-type phosphatase domainPROSITEPS51746PPM_2coord: 197..435
score: 36.677128
IPR001932PPM-type phosphatase domainCDDcd00143PP2Cccoord: 197..414
e-value: 2.38671E-53
score: 177.133
IPR036457PPM-type phosphatase domain superfamilyGENE3D3.60.40.10coord: 193..419
e-value: 7.8E-63
score: 214.6
IPR036457PPM-type phosphatase domain superfamilySUPERFAMILY81606PP2C-likecoord: 192..411
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 147..169
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 170..186
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 266..285
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 128..188
NoneNo IPR availablePANTHERPTHR47992ALPHABET, ISOFORM E-RELATEDcoord: 135..414
NoneNo IPR availablePANTHERPTHR47992:SF141PROTEIN PHOSPHATASE 2C 75-RELATEDcoord: 135..414
IPR000222PPM-type phosphatase, divalent cation bindingPROSITEPS01032PPM_1coord: 233..241

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lag0019953Lag0019953gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lag0019953.1.exon1Lag0019953.1.exon1exon
Lag0019953.1.exon2Lag0019953.1.exon2exon
Lag0019953.1.exon3Lag0019953.1.exon3exon
Lag0019953.1.exon4Lag0019953.1.exon4exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Lag0019953.1cds.Lag0019953.1CDS
cds.Lag0019953.1cds.Lag0019953.1_2CDS
cds.Lag0019953.1cds.Lag0019953.1_3CDS
cds.Lag0019953.1cds.Lag0019953.1_4CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lag0019953.1Lag0019953.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006470 protein dephosphorylation
cellular_component GO:0005829 cytosol
cellular_component GO:0005634 nucleus
molecular_function GO:0043169 cation binding
molecular_function GO:0016791 phosphatase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0106306 protein serine phosphatase activity
molecular_function GO:0106307 protein threonine phosphatase activity