Lag0017270.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0017270.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Reverse transcriptase domain-containing protein
Location	chr5: 1600183 .. 1601490 (-)
Sequence length	1308
RNA-Seq Expression	Lag0017270.1
Synteny	Lag0017270.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCCACTCGAAGAAGGAACTCCATTATGGAACTTTTATCTCGATCGGGTCAGAGTTTGGTTGATGATTCTAGCATTGAAACAGAGTTTGTGGATTTCTATAGGAAGTTATTTTCTAAAAAGGATGGCAATTGGTTTTTACCTGACATAGAAGATTGGGGTACAATATCAGATAGCCTTAGTGCTAGCCTGGAAGTTCCCTTCACCGAAAAAGAAGTCCATAGAGCTGTCAACGATTTGGGATCCAATAAATCTCCCGGCCTGGATGGTTTCACGGCTGAATTCTTTAAAAAATCATGGAACATTCTTAAGAAAGACATTATGGGAGTGTTCAATGATTTTTTTAAGAGTGCTACTATTAACGCCAACTTAAATGAGACTTATATTTGTCTTATCCCAAAGAAAATTGGAGCTAAATCGGTTGGTGACTATAGACCCATTAGCCTTACATCATGCCTCTACAAAATTGTGGCTCGTGTCTTATCAGAAAGATTGAAGAAAGTCTTGCCCCACACTATCATCGAATACCAATCTGTCTTTGTCGCAGATAGACAAATCTTAGATGCCTCTCTCGTTGCCAATGAGCTTATTGACGAGTGGCAAAGGAAAAAGGAAAAAGGAGTTTGCATCAAACTTGATATTGAAAAGGCCTTTGATATGGTTGACTGGGAATTCCTTGACGAGATTCTTCGTGTTAAGGGTTTTGGTTACACATGGAGGAGATGGATTAGGGGATGTATATCATTGGTAAACTTTTCTATTATCATAAATGGGAAACCAAGAGGAAAATTCGATGCATCTCGTGGCCTTCGACAAGGTGACCCTTTATCTCCATTCTTATTTATTATGGTTGTTGATTGCCTTAGTAGGATGCTTATCAAGGCCGAGCAACAAGATCTTATTAAGGGCCTACACGTTGGTGGGTCTCATGCTCTCTCCATCACCCATCTACAATTTGCGGATGACACAATCCTTTTCTCCTCCCCAAACGAAGCTCACCTTGACAATCTCTTCAAATCGATAAAGCTTTTTGAGGAAGCTCCTGGGTTGAATATTAATTGTTTTAAAACAGAGTTCATGGGCATTGGCTTGGATCCACAAATCCTTTATTCATTGGCTGATCGTTATGGATGCAAAATTGGTGGCTGGCCAAACACGTATTTGGGTCTTCCTTTGAATGGGAAGCCAAAGTCCTTAACTTTTTGGGCGTCTGTTTTAGAGAAAATTGAGAAAAGACTTCATTCTTGGGGATCCCAACACCTCCCGAAAGGATGTAGACTTACCCTTATACAAGCTACTCTTTAG

mRNA sequence

ATGGCTGCCACTCGAAGAAGGAACTCCATTATGGAACTTTTATCTCGATCGGGTCAGAGTTTGGTTGATGATTCTAGCATTGAAACAGAGTTTGTGGATTTCTATAGGAAGTTATTTTCTAAAAAGGATGGCAATTGGTTTTTACCTGACATAGAAGATTGGGGTACAATATCAGATAGCCTTAGTGCTAGCCTGGAAGTTCCCTTCACCGAAAAAGAAGTCCATAGAGCTGTCAACGATTTGGGATCCAATAAATCTCCCGGCCTGGATGGTTTCACGGCTGAATTCTTTAAAAAATCATGGAACATTCTTAAGAAAGACATTATGGGAGTGTTCAATGATTTTTTTAAGAGTGCTACTATTAACGCCAACTTAAATGAGACTTATATTTGTCTTATCCCAAAGAAAATTGGAGCTAAATCGGTTGGTGACTATAGACCCATTAGCCTTACATCATGCCTCTACAAAATTGTGGCTCGTGTCTTATCAGAAAGATTGAAGAAAGTCTTGCCCCACACTATCATCGAATACCAATCTGTCTTTGTCGCAGATAGACAAATCTTAGATGCCTCTCTCGTTGCCAATGAGCTTATTGACGAGTGGCAAAGGAAAAAGGAAAAAGGAGTTTGCATCAAACTTGATATTGAAAAGGCCTTTGATATGGTTGACTGGGAATTCCTTGACGAGATTCTTCGTGTTAAGGGTTTTGGTTACACATGGAGGAGATGGATTAGGGGATGTATATCATTGGTAAACTTTTCTATTATCATAAATGGGAAACCAAGAGGAAAATTCGATGCATCTCGTGGCCTTCGACAAGGTGACCCTTTATCTCCATTCTTATTTATTATGGTTGTTGATTGCCTTAGTAGGATGCTTATCAAGGCCGAGCAACAAGATCTTATTAAGGGCCTACACGTTGGTGGGTCTCATGCTCTCTCCATCACCCATCTACAATTTGCGGATGACACAATCCTTTTCTCCTCCCCAAACGAAGCTCACCTTGACAATCTCTTCAAATCGATAAAGCTTTTTGAGGAAGCTCCTGGGTTGAATATTAATTGTTTTAAAACAGAGTTCATGGGCATTGGCTTGGATCCACAAATCCTTTATTCATTGGCTGATCGTTATGGATGCAAAATTGGTGGCTGGCCAAACACGTATTTGGGTCTTCCTTTGAATGGGAAGCCAAAGTCCTTAACTTTTTGGGCGTCTGTTTTAGAGAAAATTGAGAAAAGACTTCATTCTTGGGGATCCCAACACCTCCCGAAAGGATGTAGACTTACCCTTATACAAGCTACTCTTTAG

Coding sequence (CDS)

ATGGCTGCCACTCGAAGAAGGAACTCCATTATGGAACTTTTATCTCGATCGGGTCAGAGTTTGGTTGATGATTCTAGCATTGAAACAGAGTTTGTGGATTTCTATAGGAAGTTATTTTCTAAAAAGGATGGCAATTGGTTTTTACCTGACATAGAAGATTGGGGTACAATATCAGATAGCCTTAGTGCTAGCCTGGAAGTTCCCTTCACCGAAAAAGAAGTCCATAGAGCTGTCAACGATTTGGGATCCAATAAATCTCCCGGCCTGGATGGTTTCACGGCTGAATTCTTTAAAAAATCATGGAACATTCTTAAGAAAGACATTATGGGAGTGTTCAATGATTTTTTTAAGAGTGCTACTATTAACGCCAACTTAAATGAGACTTATATTTGTCTTATCCCAAAGAAAATTGGAGCTAAATCGGTTGGTGACTATAGACCCATTAGCCTTACATCATGCCTCTACAAAATTGTGGCTCGTGTCTTATCAGAAAGATTGAAGAAAGTCTTGCCCCACACTATCATCGAATACCAATCTGTCTTTGTCGCAGATAGACAAATCTTAGATGCCTCTCTCGTTGCCAATGAGCTTATTGACGAGTGGCAAAGGAAAAAGGAAAAAGGAGTTTGCATCAAACTTGATATTGAAAAGGCCTTTGATATGGTTGACTGGGAATTCCTTGACGAGATTCTTCGTGTTAAGGGTTTTGGTTACACATGGAGGAGATGGATTAGGGGATGTATATCATTGGTAAACTTTTCTATTATCATAAATGGGAAACCAAGAGGAAAATTCGATGCATCTCGTGGCCTTCGACAAGGTGACCCTTTATCTCCATTCTTATTTATTATGGTTGTTGATTGCCTTAGTAGGATGCTTATCAAGGCCGAGCAACAAGATCTTATTAAGGGCCTACACGTTGGTGGGTCTCATGCTCTCTCCATCACCCATCTACAATTTGCGGATGACACAATCCTTTTCTCCTCCCCAAACGAAGCTCACCTTGACAATCTCTTCAAATCGATAAAGCTTTTTGAGGAAGCTCCTGGGTTGAATATTAATTGTTTTAAAACAGAGTTCATGGGCATTGGCTTGGATCCACAAATCCTTTATTCATTGGCTGATCGTTATGGATGCAAAATTGGTGGCTGGCCAAACACGTATTTGGGTCTTCCTTTGAATGGGAAGCCAAAGTCCTTAACTTTTTGGGCGTCTGTTTTAGAGAAAATTGAGAAAAGACTTCATTCTTGGGGATCCCAACACCTCCCGAAAGGATGTAGACTTACCCTTATACAAGCTACTCTTTAG

Protein sequence

MAATRRRNSIMELLSRSGQSLVDDSSIETEFVDFYRKLFSKKDGNWFLPDIEDWGTISDSLSASLEVPFTEKEVHRAVNDLGSNKSPGLDGFTAEFFKKSWNILKKDIMGVFNDFFKSATINANLNETYICLIPKKIGAKSVGDYRPISLTSCLYKIVARVLSERLKKVLPHTIIEYQSVFVADRQILDASLVANELIDEWQRKKEKGVCIKLDIEKAFDMVDWEFLDEILRVKGFGYTWRRWIRGCISLVNFSIIINGKPRGKFDASRGLRQGDPLSPFLFIMVVDCLSRMLIKAEQQDLIKGLHVGGSHALSITHLQFADDTILFSSPNEAHLDNLFKSIKLFEEAPGLNINCFKTEFMGIGLDPQILYSLADRYGCKIGGWPNTYLGLPLNGKPKSLTFWASVLEKIEKRLHSWGSQHLPKGCRLTLIQATL

Homology

[Close]

BLAST of Lag0017270.1 vs. NCBI nr
Match: BBN69746.1 (VIRB2-interacting protein 2 [Prunus dulcis])

HSP 1 Score: 431.8 bits (1109), Expect = 7.0e-117
Identity = 214/432 (49.54%), Postives = 289/432 (66.90%), Query Frame = 0


Query: 5   RRRNSIMELLSRSGQSLVDDSSIETEFVDFYRKLFSKK-DGNWFLPDIEDWGTISDSLSA 64
           R+RN I +L    G  +V +  IE E ++F++ L+S   +  W L  + +W  IS   + 
Sbjct: 127 RKRNFIQKLEVAGGGVVVSEGEIELEIINFFKNLYSSNVEAGWCLEGL-NWNAISVEEAE 186

Query: 65  SLEVPFTEKEVHRAVNDLGSNKSPGLDGFTAEFFKKSWNILKKDIMGVFNDFFKSATINA 124
            L+ PF E+EV RAV D G +KSPG DGF+   F+  W+I+K+D+M V  DFF    INA
Sbjct: 187 WLDRPFEEEEVKRAVFDCGIDKSPGPDGFSMLLFQSCWDIVKEDLMKVMADFFNCGIINA 246

Query: 125 NLNETYICLIPKKIGAKSVGDYRPISLTSCLYKIVARVLSERLKKVLPHTIIEYQSVFVA 184
             NET+ICLIPKK  +  V D+RPISL + LYK+V++VL+ RL++VL  TI  YQS FV 
Sbjct: 247 ITNETFICLIPKKKESVKVSDFRPISLVTSLYKMVSKVLASRLREVLGSTISSYQSAFVQ 306

Query: 185 DRQILDASLVANELIDEWQRKKEKGVCIKLDIEKAFDMVDWEFLDEILRVKGFGYTWRRW 244
            RQILDA+L+ANE+++E +R  + G+  K+D+EKA+D V+W F+DE+L  KGFG  WR W
Sbjct: 307 GRQILDAALIANEVVEESRRLNKSGMVFKIDLEKAYDHVEWRFVDEVLIRKGFGDRWRSW 366

Query: 245 IRGCISLVNFSIIINGKPRGKFDASRGLRQGDPLSPFLFIMVVDCLSRMLIKAEQQDLIK 304
           IRGC+   NFS++ING+PRGKF ASRGLRQGDPLSPFLF +V+D LSR++ KA+  D   
Sbjct: 367 IRGCLETANFSVMINGRPRGKFRASRGLRQGDPLSPFLFTLVMDVLSRIMEKAQDADEFH 426

Query: 305 GLHVGGSHALSITHLQFADDTILFSSPNEAHLDNLFKSIKLFEEAPGLNINCFKTEFMGI 364
           GL  G    + I+HLQFADDTI F    E + +NL + ++LF    G+ IN  K   +GI
Sbjct: 427 GLSPGNG-MVEISHLQFADDTIFFIEDKEEYWNNLLQILELFCFVSGMTINKSKCSLVGI 486

Query: 365 GLDPQILYSLADRYGCKIGGWPNTYLGLPLNGKPKSLTFWASVLEKIEKRLHSWGSQHLP 424
            LD  ++  +A  +GC +G WP  YLGLPL G P+++ FW  V+EK+E RL  W    L 
Sbjct: 487 NLDDGMVNEMAGAWGCDVGVWPMLYLGLPLGGNPRAIKFWDPVVEKVENRLQKWKRACLS 546

Query: 425 KGCRLTLIQATL 436
           KG RLT+IQA L
Sbjct: 547 KGGRLTMIQAVL 556

[Close]

BLAST of Lag0017270.1 vs. NCBI nr
Match: VVA21938.1 (Hypothetical predicted protein, partial [Prunus dulcis])

HSP 1 Score: 431.8 bits (1109), Expect = 7.0e-117
Identity = 214/432 (49.54%), Postives = 289/432 (66.90%), Query Frame = 0


Query: 5   RRRNSIMELLSRSGQSLVDDSSIETEFVDFYRKLFSKK-DGNWFLPDIEDWGTISDSLSA 64
           R+RN I +L    G  +V +  IE E ++F++ L+S   +  W L  + +W  IS   + 
Sbjct: 307 RKRNFIQKLEVAGGGVVVSEGEIELEIINFFKNLYSSNVEAGWCLEGL-NWNAISVEEAE 366

Query: 65  SLEVPFTEKEVHRAVNDLGSNKSPGLDGFTAEFFKKSWNILKKDIMGVFNDFFKSATINA 124
            L+ PF E+EV RAV D G +KSPG DGF+   F+  W+I+K+D+M V  DFF    INA
Sbjct: 367 WLDRPFEEEEVKRAVFDCGIDKSPGPDGFSMLLFQSCWDIVKEDLMKVMADFFNCGIINA 426

Query: 125 NLNETYICLIPKKIGAKSVGDYRPISLTSCLYKIVARVLSERLKKVLPHTIIEYQSVFVA 184
             NET+ICLIPKK  +  V D+RPISL + LYK+V++VL+ RL++VL  TI  YQS FV 
Sbjct: 427 ITNETFICLIPKKKESVKVSDFRPISLVTSLYKMVSKVLASRLREVLGSTISSYQSAFVQ 486

Query: 185 DRQILDASLVANELIDEWQRKKEKGVCIKLDIEKAFDMVDWEFLDEILRVKGFGYTWRRW 244
            RQILDA+L+ANE+++E +R  + G+  K+D+EKA+D V+W F+DE+L  KGFG  WR W
Sbjct: 487 GRQILDAALIANEVVEESRRLNKSGMVFKIDLEKAYDHVEWRFVDEVLIRKGFGDRWRSW 546

Query: 245 IRGCISLVNFSIIINGKPRGKFDASRGLRQGDPLSPFLFIMVVDCLSRMLIKAEQQDLIK 304
           IRGC+   NFS++ING+PRGKF ASRGLRQGDPLSPFLF +V+D LSR++ KA+  D   
Sbjct: 547 IRGCLETANFSVMINGRPRGKFRASRGLRQGDPLSPFLFTLVMDVLSRIMEKAQDADEFH 606

Query: 305 GLHVGGSHALSITHLQFADDTILFSSPNEAHLDNLFKSIKLFEEAPGLNINCFKTEFMGI 364
           GL  G    + I+HLQFADDTI F    E + +NL + ++LF    G+ IN  K   +GI
Sbjct: 607 GLSPGNG-MVEISHLQFADDTIFFIEDKEEYWNNLLQILELFCFVSGMTINKSKCSLVGI 666

Query: 365 GLDPQILYSLADRYGCKIGGWPNTYLGLPLNGKPKSLTFWASVLEKIEKRLHSWGSQHLP 424
            LD  ++  +A  +GC +G WP  YLGLPL G P+++ FW  V+EK+E RL  W    L 
Sbjct: 667 NLDDGMVNEMAGAWGCDVGVWPMLYLGLPLGGNPRAIKFWDPVVEKVENRLQKWKRACLS 726

Query: 425 KGCRLTLIQATL 436
           KG RLT+IQA L
Sbjct: 727 KGGRLTMIQAVL 736

[Close]

BLAST of Lag0017270.1 vs. NCBI nr
Match: VVA13439.1 (Hypothetical predicted protein, partial [Prunus dulcis])

HSP 1 Score: 431.8 bits (1109), Expect = 7.0e-117
Identity = 214/432 (49.54%), Postives = 289/432 (66.90%), Query Frame = 0


Query: 5   RRRNSIMELLSRSGQSLVDDSSIETEFVDFYRKLFSKK-DGNWFLPDIEDWGTISDSLSA 64
           R+RN I +L    G  +V +  IE E ++F++ L+S   +  W L  + +W  IS   + 
Sbjct: 96  RKRNFIQKLEVAGGGVVVSEGEIELEIINFFKNLYSSNVEAGWCLEGL-NWNAISVEEAE 155

Query: 65  SLEVPFTEKEVHRAVNDLGSNKSPGLDGFTAEFFKKSWNILKKDIMGVFNDFFKSATINA 124
            L+ PF E+EV RAV D G +KSPG DGF+   F+  W+I+K+D+M V  DFF    INA
Sbjct: 156 WLDRPFEEEEVKRAVFDCGIDKSPGPDGFSMLLFQSCWDIVKEDLMKVMADFFNCGIINA 215

Query: 125 NLNETYICLIPKKIGAKSVGDYRPISLTSCLYKIVARVLSERLKKVLPHTIIEYQSVFVA 184
             NET+ICLIPKK  +  V D+RPISL + LYK+V++VL+ RL++VL  TI  YQS FV 
Sbjct: 216 ITNETFICLIPKKKESVKVSDFRPISLVTSLYKMVSKVLASRLREVLGSTISSYQSAFVQ 275

Query: 185 DRQILDASLVANELIDEWQRKKEKGVCIKLDIEKAFDMVDWEFLDEILRVKGFGYTWRRW 244
            RQILDA+L+ANE+++E +R  + G+  K+D+EKA+D V+W F+DE+L  KGFG  WR W
Sbjct: 276 GRQILDAALIANEVVEESRRLNKSGMVFKIDLEKAYDHVEWRFVDEVLIRKGFGDRWRSW 335

Query: 245 IRGCISLVNFSIIINGKPRGKFDASRGLRQGDPLSPFLFIMVVDCLSRMLIKAEQQDLIK 304
           IRGC+   NFS++ING+PRGKF ASRGLRQGDPLSPFLF +V+D LSR++ KA+  D   
Sbjct: 336 IRGCLETANFSVMINGRPRGKFRASRGLRQGDPLSPFLFTLVMDVLSRIMEKAQDADEFH 395

Query: 305 GLHVGGSHALSITHLQFADDTILFSSPNEAHLDNLFKSIKLFEEAPGLNINCFKTEFMGI 364
           GL  G    + I+HLQFADDTI F    E + +NL + ++LF    G+ IN  K   +GI
Sbjct: 396 GLSPGNG-MVEISHLQFADDTIFFIEDKEEYWNNLLQILELFCFVSGMTINKSKCSLVGI 455

Query: 365 GLDPQILYSLADRYGCKIGGWPNTYLGLPLNGKPKSLTFWASVLEKIEKRLHSWGSQHLP 424
            LD  ++  +A  +GC +G WP  YLGLPL G P+++ FW  V+EK+E RL  W    L 
Sbjct: 456 NLDDGMVNEMAGAWGCDVGVWPMLYLGLPLGGNPRAIKFWDPVVEKVENRLQKWKRACLS 515

Query: 425 KGCRLTLIQATL 436
           KG RLT+IQA L
Sbjct: 516 KGGRLTMIQAVL 525

[Close]

BLAST of Lag0017270.1 vs. NCBI nr
Match: VVA41200.1 (PREDICTED: RNA-directed DNA polymerase, partial [Prunus dulcis])

HSP 1 Score: 431.8 bits (1109), Expect = 7.0e-117
Identity = 214/432 (49.54%), Postives = 289/432 (66.90%), Query Frame = 0


Query: 5   RRRNSIMELLSRSGQSLVDDSSIETEFVDFYRKLFSKK-DGNWFLPDIEDWGTISDSLSA 64
           R+RN I +L    G  +V +  IE E ++F++ L+S   +  W L  + +W  IS   + 
Sbjct: 307 RKRNFIQKLEVAGGGVVVSEGEIELEIINFFKNLYSSNVEAGWCLEGL-NWNAISVEEAE 366

Query: 65  SLEVPFTEKEVHRAVNDLGSNKSPGLDGFTAEFFKKSWNILKKDIMGVFNDFFKSATINA 124
            L+ PF E+EV RAV D G +KSPG DGF+   F+  W+I+K+D+M V  DFF    INA
Sbjct: 367 WLDRPFEEEEVKRAVFDCGIDKSPGPDGFSMLLFQSCWDIVKEDLMKVMADFFNCGIINA 426

Query: 125 NLNETYICLIPKKIGAKSVGDYRPISLTSCLYKIVARVLSERLKKVLPHTIIEYQSVFVA 184
             NET+ICLIPKK  +  V D+RPISL + LYK+V++VL+ RL++VL  TI  YQS FV 
Sbjct: 427 ITNETFICLIPKKKESVKVSDFRPISLVTSLYKMVSKVLASRLREVLGSTISSYQSAFVQ 486

Query: 185 DRQILDASLVANELIDEWQRKKEKGVCIKLDIEKAFDMVDWEFLDEILRVKGFGYTWRRW 244
            RQILDA+L+ANE+++E +R  + G+  K+D+EKA+D V+W F+DE+L  KGFG  WR W
Sbjct: 487 GRQILDAALIANEVVEESRRLNKSGMVFKIDLEKAYDHVEWRFVDEVLIRKGFGDRWRSW 546

Query: 245 IRGCISLVNFSIIINGKPRGKFDASRGLRQGDPLSPFLFIMVVDCLSRMLIKAEQQDLIK 304
           IRGC+   NFS++ING+PRGKF ASRGLRQGDPLSPFLF +V+D LSR++ KA+  D   
Sbjct: 547 IRGCLETANFSVMINGRPRGKFRASRGLRQGDPLSPFLFTLVMDVLSRIMEKAQDADEFH 606

Query: 305 GLHVGGSHALSITHLQFADDTILFSSPNEAHLDNLFKSIKLFEEAPGLNINCFKTEFMGI 364
           GL  G    + I+HLQFADDTI F    E + +NL + ++LF    G+ IN  K   +GI
Sbjct: 607 GLSPGNG-MVEISHLQFADDTIFFIEDKEEYWNNLLQILELFCFVSGMTINKSKCSLVGI 666

Query: 365 GLDPQILYSLADRYGCKIGGWPNTYLGLPLNGKPKSLTFWASVLEKIEKRLHSWGSQHLP 424
            LD  ++  +A  +GC +G WP  YLGLPL G P+++ FW  V+EK+E RL  W    L 
Sbjct: 667 NLDDGMVNEMAGAWGCDVGVWPMLYLGLPLGGNPRAIKFWDPVVEKVENRLQKWKRACLS 726

Query: 425 KGCRLTLIQATL 436
           KG RLT+IQA L
Sbjct: 727 KGGRLTMIQAVL 736

[Close]

BLAST of Lag0017270.1 vs. NCBI nr
Match: BBG98819.1 (VIRB2-interacting protein 2 [Prunus dulcis] >BBH09158.1 VIRB2-interacting protein 2 [Prunus dulcis])

HSP 1 Score: 431.8 bits (1109), Expect = 7.0e-117
Identity = 214/432 (49.54%), Postives = 289/432 (66.90%), Query Frame = 0


Query: 5   RRRNSIMELLSRSGQSLVDDSSIETEFVDFYRKLFSKK-DGNWFLPDIEDWGTISDSLSA 64
           R+RN I +L    G  +V +  IE E ++F++ L+S   +  W L  + +W  IS   + 
Sbjct: 60  RKRNFIQKLEVAGGGVVVSEGEIELEIINFFKNLYSSNVEAGWCLEGL-NWNAISVEEAE 119

Query: 65  SLEVPFTEKEVHRAVNDLGSNKSPGLDGFTAEFFKKSWNILKKDIMGVFNDFFKSATINA 124
            L+ PF E+EV RAV D G +KSPG DGF+   F+  W+I+K+D+M V  DFF    INA
Sbjct: 120 WLDRPFEEEEVKRAVFDCGIDKSPGPDGFSMLLFQSCWDIVKEDLMKVMADFFNCGIINA 179

Query: 125 NLNETYICLIPKKIGAKSVGDYRPISLTSCLYKIVARVLSERLKKVLPHTIIEYQSVFVA 184
             NET+ICLIPKK  +  V D+RPISL + LYK+V++VL+ RL++VL  TI  YQS FV 
Sbjct: 180 ITNETFICLIPKKKESVKVSDFRPISLVTSLYKMVSKVLASRLREVLGSTISSYQSAFVQ 239

Query: 185 DRQILDASLVANELIDEWQRKKEKGVCIKLDIEKAFDMVDWEFLDEILRVKGFGYTWRRW 244
            RQILDA+L+ANE+++E +R  + G+  K+D+EKA+D V+W F+DE+L  KGFG  WR W
Sbjct: 240 GRQILDAALIANEVVEESRRLNKSGMVFKIDLEKAYDHVEWRFVDEVLIRKGFGDRWRSW 299

Query: 245 IRGCISLVNFSIIINGKPRGKFDASRGLRQGDPLSPFLFIMVVDCLSRMLIKAEQQDLIK 304
           IRGC+   NFS++ING+PRGKF ASRGLRQGDPLSPFLF +V+D LSR++ KA+  D   
Sbjct: 300 IRGCLETANFSVMINGRPRGKFRASRGLRQGDPLSPFLFTLVMDVLSRIMEKAQDADEFH 359

Query: 305 GLHVGGSHALSITHLQFADDTILFSSPNEAHLDNLFKSIKLFEEAPGLNINCFKTEFMGI 364
           GL  G    + I+HLQFADDTI F    E + +NL + ++LF    G+ IN  K   +GI
Sbjct: 360 GLSPGNG-MVEISHLQFADDTIFFIEDKEEYWNNLLQILELFCFVSGMTINKSKCSLVGI 419

Query: 365 GLDPQILYSLADRYGCKIGGWPNTYLGLPLNGKPKSLTFWASVLEKIEKRLHSWGSQHLP 424
            LD  ++  +A  +GC +G WP  YLGLPL G P+++ FW  V+EK+E RL  W    L 
Sbjct: 420 NLDDGMVNEMAGAWGCDVGVWPMLYLGLPLGGNPRAIKFWDPVVEKVENRLQKWKRACLS 479

Query: 425 KGCRLTLIQATL 436
           KG RLT+IQA L
Sbjct: 480 KGGRLTMIQAVL 489

[Close]

BLAST of Lag0017270.1 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 4.8e-36
Identity = 121/427 (28.34%), Postives = 195/427 (45.67%), Query Frame = 0


Query: 5   RRRNSIMELLSRSGQSLVDDSSIETEFVDFYRKLFSKKDGNWFLPDIEDWGTISDSLS-- 64
           R +N I  + +  G    D + I+T   ++Y+ L++ K     L ++E+  T  D+ +  
Sbjct: 385 REKNQIDTIKNDKGDITTDPTEIQTTIREYYKHLYANK-----LENLEEMDTFLDTYTLP 444

Query: 65  -------ASLEVPFTEKEVHRAVNDLGSNKSPGLDGFTAEFFKKSWNILKKDIMGVFNDF 124
                   SL  P T  E+   +N L + KSPG DGFTAEF+++    L   ++ +F   
Sbjct: 445 RLNQEEVESLNRPITGSEIVAIINSLPTKKSPGPDGFTAEFYQRYKEELVPFLLKLFQSI 504

Query: 125 FKSATINANLNETYICLIPKK-IGAKSVGDYRPISLTSCLYKIVARVLSERLKKVLPHTI 184
            K   +  +  E  I LIPK         ++RPISL +   KI+ ++L+ R+++ +   I
Sbjct: 505 EKEGILPNSFYEASIILIPKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQHIKKLI 564

Query: 185 IEYQSVFVADRQILDASLVANELIDEWQRKKEKG-VCIKLDIEKAFDMVDWEFLDEILRV 244
              Q  F+   Q       +  +I    R K+K  V I +D EKAFD +   F+ + L  
Sbjct: 565 HHDQVGFIPGMQGWFNIRKSINVIQHINRAKDKNHVIISIDAEKAFDKIQQPFMLKTLNK 624

Query: 245 KGFGYTWRRWIRGCISLVNFSIIINGKPRGKFDASRGLRQGDPLSPFLFIMVVDCLSRML 304
            G    + + IR        +II+NG+    F    G RQG PLSP LF +V++ L+R +
Sbjct: 625 LGIDGMYLKIIRAIYDKPTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLEVLARAI 684

Query: 305 IKAEQQDLIKGLHVGGSHA-LSITHLQFADDTILFSSPNEAHLDNLFKSIKLFEEAPGLN 364
               Q+  IKG+ +G     LS+    FADD I++         NL K I  F +  G  
Sbjct: 685 ---RQEKEIKGIQLGKEEVKLSL----FADDMIVYLENPIVSAQNLLKLISNFSKVSGYK 744

Query: 365 INCFKTEFMGIGLDPQILYSLADRYGCKIGGWPNTYLGLPLNGKPKSL--TFWASVLEKI 418
           IN  K++      + Q    +       I      YLG+ L    K L    +  +L++I
Sbjct: 745 INVQKSQAFLYNNNRQTESQIMGELPFTIASKRIKYLGIQLTRDVKDLFKENYKPLLKEI 799

[Close]

BLAST of Lag0017270.1 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 7.2e-32
Identity = 97/293 (33.11%), Postives = 139/293 (47.44%), Query Frame = 0


Query: 7   RNSIMELLSRSGQSLVDDSSIETEFVDFYRKLFSKKDGNWFLPDI--EDWG---TISDSL 66
           R  I  L +  G  L D  +I      FY+ LFS        PD   E W     +S+  
Sbjct: 385 RKQITCLFAEDGTPLEDPEAIRDRARSFYQNLFSPDP---ISPDACEELWDGLPVVSERR 444

Query: 67  SASLEVPFTEKEVHRAVNDLGSNKSPGLDGFTAEFFKKSWNILKKDIMGVFNDFFKSATI 126
              LE P T  E+ +A+  +  NKSPGLDG T EFF+  W+ L  D   V  + FK   +
Sbjct: 445 KERLETPITLDELSQALRLMPHNKSPGLDGLTIEFFQFFWDTLGPDFHRVLTEAFKKGEL 504

Query: 127 NANLNETYICLIPKKIGAKSVGDYRPISLTSCLYKIVARVLSERLKKVLPHTIIEYQSVF 186
             +     + L+PKK   + + ++RP+SL S  YKIVA+ +S RLK VL   I   QS  
Sbjct: 505 PLSCRRAVLSLLPKKGDLRLIKNWRPVSLLSTDYKIVAKAISLRLKSVLAEVIHPDQSYT 564

Query: 187 VADRQILDASLVANELIDEWQRKKEKGVCIKLDIEKAFDMVDWEFLDEILRVKGFGYTWR 246
           V  R I D   +  +L+   +R       + LD EKAFD VD ++L   L+   FG  + 
Sbjct: 565 VPGRTIFDNVFLIRDLLHFARRTGLSLAFLSLDQEKAFDRVDHQYLIGTLQAYSFGPQFV 624

Query: 247 RWIRGCISLVNFSIIINGKPRGKFDASRGLRQGDPLSPFLFIMVVD---CLSR 292
            +++   +     + IN          RG+RQG PLS  L+ + ++   CL R
Sbjct: 625 GYLKTMYASAECLVKINWSLTAPLAFGRGVRQGCPLSGQLYSLAIEPFLCLLR 674

[Close]

BLAST of Lag0017270.1 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 136.3 bits (342), Expect = 8.0e-31
Identity = 114/425 (26.82%), Postives = 193/425 (45.41%), Query Frame = 0


Query: 10  IMELLSRSGQSLVDDSSIETEFVDFYRKLFSKKDGNWFLPDIEDWGTISDSLSA------ 69
           I ++ +  G    D   I+     FY++L+S K     L ++++     D          
Sbjct: 397 INKIRNEKGDITTDPEEIQNTIRSFYKRLYSTK-----LENLDEMDKFLDRYQVPKLNQD 456

Query: 70  ---SLEVPFTEKEVHRAVNDLGSNKSPGLDGFTAEFFKKSWNILKKDIMGVFNDFFKSAT 129
               L  P + KE+   +N L + KSPG DGF+AEF++      K+D++ + +  F    
Sbjct: 457 QVDHLNSPISPKEIEAVINSLPTKKSPGPDGFSAEFYQ----TFKEDLIPILHKLFHKIE 516

Query: 130 INANLNETY----ICLIPK-KIGAKSVGDYRPISLTSCLYKIVARVLSERLKKVLPHTII 189
           +   L  ++    I LIPK +     + ++RPISL +   KI+ ++L+ R+++ +   I 
Sbjct: 517 VEGTLPNSFYEATITLIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQEHIKAIIH 576

Query: 190 EYQSVFVADRQILDASLVANELIDEWQRKKEKG-VCIKLDIEKAFDMVDWEFLDEILRVK 249
             Q  F+   Q       +  +I    + K+K  + I LD EKAFD +   F+ ++L   
Sbjct: 577 PDQVGFIPGMQGWFNIRKSINVIHYINKLKDKNHMIISLDAEKAFDKIQHPFMIKVLERS 636

Query: 250 GFGYTWRRWIRGCISLVNFSIIINGKPRGKFDASRGLRQGDPLSPFLFIMVVDCLSRMLI 309
           G    +   I+   S    +I +NG+         G RQG PLSP+LF +V++ L+R + 
Sbjct: 637 GIQGPYLNMIKAIYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLARAI- 696

Query: 310 KAEQQDLIKGLHVGGSHALSITHLQFADDTILFSSPNEAHLDNLFKSIKLFEEAPGLNIN 369
              QQ  IKG+ + G   + I+ L  ADD I++ S  +     L   I  F E  G  IN
Sbjct: 697 --RQQKEIKGIQI-GKEEVKISLL--ADDMIVYISDPKNSTRELLNLINSFGEVVGYKIN 756

Query: 370 CFKTEFMGIGLDPQILYSLADRYGCKIGGWPNTYLGLPLNGKPKSL--TFWASVLEKIEK 418
             K+       + Q    + +     I      YLG+ L  + K L    + S+ ++I++
Sbjct: 757 SNKSMAFLYTKNKQAEKEIRETTPFSIVTNNIKYLGVTLTKEVKDLYDKNFKSLKKEIKE 806

[Close]

BLAST of Lag0017270.1 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 4.0e-30
Identity = 106/370 (28.65%), Postives = 170/370 (45.95%), Query Frame = 0


Query: 1   MAATRRRNSIMELLS--RSGQSLV--DDSSIETEFVDFYRKLFSKKDGNWFLPDIEDW-- 60
           +A   R+  +  L+S  R+G   +  D S I+    ++Y+KL+S K  N  L +I+ +  
Sbjct: 376 LANLTRKKRVKSLISSIRNGNDEITTDPSEIQKILNEYYKKLYSHKYEN--LKEIDQYLE 435

Query: 61  ----GTISDSLSASLEVPFTEKEVHRAVNDLGSNKSPGLDGFTAEFFKKSWNILKKDIMG 120
                 +S      L  P +  E+   + +L   KSPG DGFT+EF++     L   ++ 
Sbjct: 436 ACHLPRLSQKEVEMLNRPISSSEIASTIQNLPKKKSPGPDGFTSEFYQTFKEELVPILLN 495

Query: 121 VFNDFFKSATINANLNETYICLIPKK-IGAKSVGDYRPISLTSCLYKIVARVLSERLKKV 180
           +F +  K   +     E  I LIPK         +YRPISL +   KI+ ++L+ R+++ 
Sbjct: 496 LFQNIEKEGILPNTFYEANITLIPKPGKDPTRKENYRPISLMNIDAKILNKILTNRIQQH 555

Query: 181 LPHTIIEYQSVFVADRQILDASLVANELIDEWQRKKEKG-VCIKLDIEKAFDMVDWEFLD 240
           +   I   Q  F+   Q       +  +I    + K K  + + +D EKAFD +   F+ 
Sbjct: 556 IKKIIHHDQVGFIPGSQGWFNIRKSINVIQHINKLKNKDHMILSIDAEKAFDNIQHPFMI 615

Query: 241 EILRVKGFGYTWRRWIRGCISLVNFSIIINGKPRGKFDASRGLRQGDPLSPFLFIMVVDC 300
             L+  G   T+ + I    S    +II+NG     F    G RQG PLSP LF +V++ 
Sbjct: 616 RTLKKIGIEGTFLKLIEAIYSKPTANIILNGVKLKSFPLRSGTRQGCPLSPLLFNIVMEV 675

Query: 301 LSRMLIKAEQQDLIKGLHVGGSHALSITHLQFADDTILFSSPNEAHLDNLFKSIKLFEEA 359
           L+   I   ++  IKG+H+G      I    FADD I++          L + IK +   
Sbjct: 676 LA---IAIREEKAIKGIHIGSE---EIKLSLFADDMIVYLENTRDSTTKLLEVIKEYSNV 735

[Close]

BLAST of Lag0017270.1 vs. ExPASy Swiss-Prot
Match: Q03274 (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica OX=7064 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.5e-13
Identity = 64/228 (28.07%), Postives = 108/228 (47.37%), Query Frame = 0


Query: 132 LIPKKIGAKSVGDYRPISLTSCLYKIVARVLSERLKKVLPHTIIEYQSVFVADRQILDAS 191
           LIPK    ++  ++RPI++ S L +++ R+L++RL+  +   +   Q  +      L  S
Sbjct: 63  LIPKDGDLENPSNWRPITIASALQRLLHRILAKRLEAAV--ELHPAQKGYARIDGTLVNS 122

Query: 192 LVANELIDEWQRKKEKGVCIKLDIEKAFDMVDWEFLDEILRVKGFGYTWRRWIRGCISLV 251
           L+ +  I   + +++    + LD+ KAFD V    +   L+  G       +I G +S  
Sbjct: 123 LLLDTYISSRREQRKTYNVVSLDVRKAFDTVSHSSICRALQRLGIDEGTSNYITGSLSDS 182

Query: 252 NFSIIIN-GKPRGKFDASRGLRQGDPLSPFLFIMVVDCLSRMLIKAEQQDLIKGLHVGGS 311
             +I +  G    K    RG++QGDPLSPFLF  V+D    +L   +    I G  +G  
Sbjct: 183 TTTIRVGPGSQTRKICIRRGVKQGDPLSPFLFNAVLD---ELLCSLQSTPGIGGT-IGEE 242

Query: 312 HALSITHLQFADDTILFSSPNEAHLDNLFKSIKLFEEAPGLNINCFKT 359
               I  L FADD +L    N+  L     ++  F    G+++N  K+
Sbjct: 243 ---KIPVLAFADDLLLLED-NDVLLPTTLATVANFFRLRGMSLNAKKS 280

[Close]

BLAST of Lag0017270.1 vs. ExPASy TrEMBL
Match: A0A4Y1R3V4 (VIRB2-interacting protein 2 OS=Prunus dulcis OX=3755 GN=Prudu_008314 PE=4 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 3.4e-117
Identity = 214/432 (49.54%), Postives = 289/432 (66.90%), Query Frame = 0


Query: 5   RRRNSIMELLSRSGQSLVDDSSIETEFVDFYRKLFSKK-DGNWFLPDIEDWGTISDSLSA 64
           R+RN I +L    G  +V +  IE E ++F++ L+S   +  W L  + +W  IS   + 
Sbjct: 60  RKRNFIQKLEVAGGGVVVSEGEIELEIINFFKNLYSSNVEAGWCLEGL-NWNAISVEEAE 119

Query: 65  SLEVPFTEKEVHRAVNDLGSNKSPGLDGFTAEFFKKSWNILKKDIMGVFNDFFKSATINA 124
            L+ PF E+EV RAV D G +KSPG DGF+   F+  W+I+K+D+M V  DFF    INA
Sbjct: 120 WLDRPFEEEEVKRAVFDCGIDKSPGPDGFSMLLFQSCWDIVKEDLMKVMADFFNCGIINA 179

Query: 125 NLNETYICLIPKKIGAKSVGDYRPISLTSCLYKIVARVLSERLKKVLPHTIIEYQSVFVA 184
             NET+ICLIPKK  +  V D+RPISL + LYK+V++VL+ RL++VL  TI  YQS FV 
Sbjct: 180 ITNETFICLIPKKKESVKVSDFRPISLVTSLYKMVSKVLASRLREVLGSTISSYQSAFVQ 239

Query: 185 DRQILDASLVANELIDEWQRKKEKGVCIKLDIEKAFDMVDWEFLDEILRVKGFGYTWRRW 244
            RQILDA+L+ANE+++E +R  + G+  K+D+EKA+D V+W F+DE+L  KGFG  WR W
Sbjct: 240 GRQILDAALIANEVVEESRRLNKSGMVFKIDLEKAYDHVEWRFVDEVLIRKGFGDRWRSW 299

Query: 245 IRGCISLVNFSIIINGKPRGKFDASRGLRQGDPLSPFLFIMVVDCLSRMLIKAEQQDLIK 304
           IRGC+   NFS++ING+PRGKF ASRGLRQGDPLSPFLF +V+D LSR++ KA+  D   
Sbjct: 300 IRGCLETANFSVMINGRPRGKFRASRGLRQGDPLSPFLFTLVMDVLSRIMEKAQDADEFH 359

Query: 305 GLHVGGSHALSITHLQFADDTILFSSPNEAHLDNLFKSIKLFEEAPGLNINCFKTEFMGI 364
           GL  G    + I+HLQFADDTI F    E + +NL + ++LF    G+ IN  K   +GI
Sbjct: 360 GLSPGNG-MVEISHLQFADDTIFFIEDKEEYWNNLLQILELFCFVSGMTINKSKCSLVGI 419

Query: 365 GLDPQILYSLADRYGCKIGGWPNTYLGLPLNGKPKSLTFWASVLEKIEKRLHSWGSQHLP 424
            LD  ++  +A  +GC +G WP  YLGLPL G P+++ FW  V+EK+E RL  W    L 
Sbjct: 420 NLDDGMVNEMAGAWGCDVGVWPMLYLGLPLGGNPRAIKFWDPVVEKVENRLQKWKRACLS 479

Query: 425 KGCRLTLIQATL 436
           KG RLT+IQA L
Sbjct: 480 KGGRLTMIQAVL 489

[Close]

BLAST of Lag0017270.1 vs. ExPASy TrEMBL
Match: A0A5E4GN72 (PREDICTED: RNA-directed DNA polymerase (Fragment) OS=Prunus dulcis OX=3755 GN=ALMOND_2B014918 PE=4 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 3.4e-117
Identity = 214/432 (49.54%), Postives = 289/432 (66.90%), Query Frame = 0


Query: 5   RRRNSIMELLSRSGQSLVDDSSIETEFVDFYRKLFSKK-DGNWFLPDIEDWGTISDSLSA 64
           R+RN I +L    G  +V +  IE E ++F++ L+S   +  W L  + +W  IS   + 
Sbjct: 307 RKRNFIQKLEVAGGGVVVSEGEIELEIINFFKNLYSSNVEAGWCLEGL-NWNAISVEEAE 366

Query: 65  SLEVPFTEKEVHRAVNDLGSNKSPGLDGFTAEFFKKSWNILKKDIMGVFNDFFKSATINA 124
            L+ PF E+EV RAV D G +KSPG DGF+   F+  W+I+K+D+M V  DFF    INA
Sbjct: 367 WLDRPFEEEEVKRAVFDCGIDKSPGPDGFSMLLFQSCWDIVKEDLMKVMADFFNCGIINA 426

Query: 125 NLNETYICLIPKKIGAKSVGDYRPISLTSCLYKIVARVLSERLKKVLPHTIIEYQSVFVA 184
             NET+ICLIPKK  +  V D+RPISL + LYK+V++VL+ RL++VL  TI  YQS FV 
Sbjct: 427 ITNETFICLIPKKKESVKVSDFRPISLVTSLYKMVSKVLASRLREVLGSTISSYQSAFVQ 486

Query: 185 DRQILDASLVANELIDEWQRKKEKGVCIKLDIEKAFDMVDWEFLDEILRVKGFGYTWRRW 244
            RQILDA+L+ANE+++E +R  + G+  K+D+EKA+D V+W F+DE+L  KGFG  WR W
Sbjct: 487 GRQILDAALIANEVVEESRRLNKSGMVFKIDLEKAYDHVEWRFVDEVLIRKGFGDRWRSW 546

Query: 245 IRGCISLVNFSIIINGKPRGKFDASRGLRQGDPLSPFLFIMVVDCLSRMLIKAEQQDLIK 304
           IRGC+   NFS++ING+PRGKF ASRGLRQGDPLSPFLF +V+D LSR++ KA+  D   
Sbjct: 547 IRGCLETANFSVMINGRPRGKFRASRGLRQGDPLSPFLFTLVMDVLSRIMEKAQDADEFH 606

Query: 305 GLHVGGSHALSITHLQFADDTILFSSPNEAHLDNLFKSIKLFEEAPGLNINCFKTEFMGI 364
           GL  G    + I+HLQFADDTI F    E + +NL + ++LF    G+ IN  K   +GI
Sbjct: 607 GLSPGNG-MVEISHLQFADDTIFFIEDKEEYWNNLLQILELFCFVSGMTINKSKCSLVGI 666

Query: 365 GLDPQILYSLADRYGCKIGGWPNTYLGLPLNGKPKSLTFWASVLEKIEKRLHSWGSQHLP 424
            LD  ++  +A  +GC +G WP  YLGLPL G P+++ FW  V+EK+E RL  W    L 
Sbjct: 667 NLDDGMVNEMAGAWGCDVGVWPMLYLGLPLGGNPRAIKFWDPVVEKVENRLQKWKRACLS 726

Query: 425 KGCRLTLIQATL 436
           KG RLT+IQA L
Sbjct: 727 KGGRLTMIQAVL 736

[Close]

BLAST of Lag0017270.1 vs. ExPASy TrEMBL
Match: A0A5H2Y6K0 (VIRB2-interacting protein 2 OS=Prunus dulcis OX=3755 GN=Prudu_1151S000200 PE=4 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 3.4e-117
Identity = 214/432 (49.54%), Postives = 289/432 (66.90%), Query Frame = 0


Query: 5   RRRNSIMELLSRSGQSLVDDSSIETEFVDFYRKLFSKK-DGNWFLPDIEDWGTISDSLSA 64
           R+RN I +L    G  +V +  IE E ++F++ L+S   +  W L  + +W  IS   + 
Sbjct: 127 RKRNFIQKLEVAGGGVVVSEGEIELEIINFFKNLYSSNVEAGWCLEGL-NWNAISVEEAE 186

Query: 65  SLEVPFTEKEVHRAVNDLGSNKSPGLDGFTAEFFKKSWNILKKDIMGVFNDFFKSATINA 124
            L+ PF E+EV RAV D G +KSPG DGF+   F+  W+I+K+D+M V  DFF    INA
Sbjct: 187 WLDRPFEEEEVKRAVFDCGIDKSPGPDGFSMLLFQSCWDIVKEDLMKVMADFFNCGIINA 246

Query: 125 NLNETYICLIPKKIGAKSVGDYRPISLTSCLYKIVARVLSERLKKVLPHTIIEYQSVFVA 184
             NET+ICLIPKK  +  V D+RPISL + LYK+V++VL+ RL++VL  TI  YQS FV 
Sbjct: 247 ITNETFICLIPKKKESVKVSDFRPISLVTSLYKMVSKVLASRLREVLGSTISSYQSAFVQ 306

Query: 185 DRQILDASLVANELIDEWQRKKEKGVCIKLDIEKAFDMVDWEFLDEILRVKGFGYTWRRW 244
            RQILDA+L+ANE+++E +R  + G+  K+D+EKA+D V+W F+DE+L  KGFG  WR W
Sbjct: 307 GRQILDAALIANEVVEESRRLNKSGMVFKIDLEKAYDHVEWRFVDEVLIRKGFGDRWRSW 366

Query: 245 IRGCISLVNFSIIINGKPRGKFDASRGLRQGDPLSPFLFIMVVDCLSRMLIKAEQQDLIK 304
           IRGC+   NFS++ING+PRGKF ASRGLRQGDPLSPFLF +V+D LSR++ KA+  D   
Sbjct: 367 IRGCLETANFSVMINGRPRGKFRASRGLRQGDPLSPFLFTLVMDVLSRIMEKAQDADEFH 426

Query: 305 GLHVGGSHALSITHLQFADDTILFSSPNEAHLDNLFKSIKLFEEAPGLNINCFKTEFMGI 364
           GL  G    + I+HLQFADDTI F    E + +NL + ++LF    G+ IN  K   +GI
Sbjct: 427 GLSPGNG-MVEISHLQFADDTIFFIEDKEEYWNNLLQILELFCFVSGMTINKSKCSLVGI 486

Query: 365 GLDPQILYSLADRYGCKIGGWPNTYLGLPLNGKPKSLTFWASVLEKIEKRLHSWGSQHLP 424
            LD  ++  +A  +GC +G WP  YLGLPL G P+++ FW  V+EK+E RL  W    L 
Sbjct: 487 NLDDGMVNEMAGAWGCDVGVWPMLYLGLPLGGNPRAIKFWDPVVEKVENRLQKWKRACLS 546

Query: 425 KGCRLTLIQATL 436
           KG RLT+IQA L
Sbjct: 547 KGGRLTMIQAVL 556

[Close]

BLAST of Lag0017270.1 vs. ExPASy TrEMBL
Match: A0A5E4F859 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus dulcis OX=3755 GN=ALMOND_2B035883 PE=4 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 3.4e-117
Identity = 214/432 (49.54%), Postives = 289/432 (66.90%), Query Frame = 0


Query: 5   RRRNSIMELLSRSGQSLVDDSSIETEFVDFYRKLFSKK-DGNWFLPDIEDWGTISDSLSA 64
           R+RN I +L    G  +V +  IE E ++F++ L+S   +  W L  + +W  IS   + 
Sbjct: 307 RKRNFIQKLEVAGGGVVVSEGEIELEIINFFKNLYSSNVEAGWCLEGL-NWNAISVEEAE 366

Query: 65  SLEVPFTEKEVHRAVNDLGSNKSPGLDGFTAEFFKKSWNILKKDIMGVFNDFFKSATINA 124
            L+ PF E+EV RAV D G +KSPG DGF+   F+  W+I+K+D+M V  DFF    INA
Sbjct: 367 WLDRPFEEEEVKRAVFDCGIDKSPGPDGFSMLLFQSCWDIVKEDLMKVMADFFNCGIINA 426

Query: 125 NLNETYICLIPKKIGAKSVGDYRPISLTSCLYKIVARVLSERLKKVLPHTIIEYQSVFVA 184
             NET+ICLIPKK  +  V D+RPISL + LYK+V++VL+ RL++VL  TI  YQS FV 
Sbjct: 427 ITNETFICLIPKKKESVKVSDFRPISLVTSLYKMVSKVLASRLREVLGSTISSYQSAFVQ 486

Query: 185 DRQILDASLVANELIDEWQRKKEKGVCIKLDIEKAFDMVDWEFLDEILRVKGFGYTWRRW 244
            RQILDA+L+ANE+++E +R  + G+  K+D+EKA+D V+W F+DE+L  KGFG  WR W
Sbjct: 487 GRQILDAALIANEVVEESRRLNKSGMVFKIDLEKAYDHVEWRFVDEVLIRKGFGDRWRSW 546

Query: 245 IRGCISLVNFSIIINGKPRGKFDASRGLRQGDPLSPFLFIMVVDCLSRMLIKAEQQDLIK 304
           IRGC+   NFS++ING+PRGKF ASRGLRQGDPLSPFLF +V+D LSR++ KA+  D   
Sbjct: 547 IRGCLETANFSVMINGRPRGKFRASRGLRQGDPLSPFLFTLVMDVLSRIMEKAQDADEFH 606

Query: 305 GLHVGGSHALSITHLQFADDTILFSSPNEAHLDNLFKSIKLFEEAPGLNINCFKTEFMGI 364
           GL  G    + I+HLQFADDTI F    E + +NL + ++LF    G+ IN  K   +GI
Sbjct: 607 GLSPGNG-MVEISHLQFADDTIFFIEDKEEYWNNLLQILELFCFVSGMTINKSKCSLVGI 666

Query: 365 GLDPQILYSLADRYGCKIGGWPNTYLGLPLNGKPKSLTFWASVLEKIEKRLHSWGSQHLP 424
            LD  ++  +A  +GC +G WP  YLGLPL G P+++ FW  V+EK+E RL  W    L 
Sbjct: 667 NLDDGMVNEMAGAWGCDVGVWPMLYLGLPLGGNPRAIKFWDPVVEKVENRLQKWKRACLS 726

Query: 425 KGCRLTLIQATL 436
           KG RLT+IQA L
Sbjct: 727 KGGRLTMIQAVL 736

[Close]

BLAST of Lag0017270.1 vs. ExPASy TrEMBL
Match: A0A5E4EEP2 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus dulcis OX=3755 GN=ALMOND_2B035545 PE=4 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 3.4e-117
Identity = 214/432 (49.54%), Postives = 289/432 (66.90%), Query Frame = 0


Query: 5   RRRNSIMELLSRSGQSLVDDSSIETEFVDFYRKLFSKK-DGNWFLPDIEDWGTISDSLSA 64
           R+RN I +L    G  +V +  IE E ++F++ L+S   +  W L  + +W  IS   + 
Sbjct: 96  RKRNFIQKLEVAGGGVVVSEGEIELEIINFFKNLYSSNVEAGWCLEGL-NWNAISVEEAE 155

Query: 65  SLEVPFTEKEVHRAVNDLGSNKSPGLDGFTAEFFKKSWNILKKDIMGVFNDFFKSATINA 124
            L+ PF E+EV RAV D G +KSPG DGF+   F+  W+I+K+D+M V  DFF    INA
Sbjct: 156 WLDRPFEEEEVKRAVFDCGIDKSPGPDGFSMLLFQSCWDIVKEDLMKVMADFFNCGIINA 215

Query: 125 NLNETYICLIPKKIGAKSVGDYRPISLTSCLYKIVARVLSERLKKVLPHTIIEYQSVFVA 184
             NET+ICLIPKK  +  V D+RPISL + LYK+V++VL+ RL++VL  TI  YQS FV 
Sbjct: 216 ITNETFICLIPKKKESVKVSDFRPISLVTSLYKMVSKVLASRLREVLGSTISSYQSAFVQ 275

Query: 185 DRQILDASLVANELIDEWQRKKEKGVCIKLDIEKAFDMVDWEFLDEILRVKGFGYTWRRW 244
            RQILDA+L+ANE+++E +R  + G+  K+D+EKA+D V+W F+DE+L  KGFG  WR W
Sbjct: 276 GRQILDAALIANEVVEESRRLNKSGMVFKIDLEKAYDHVEWRFVDEVLIRKGFGDRWRSW 335

Query: 245 IRGCISLVNFSIIINGKPRGKFDASRGLRQGDPLSPFLFIMVVDCLSRMLIKAEQQDLIK 304
           IRGC+   NFS++ING+PRGKF ASRGLRQGDPLSPFLF +V+D LSR++ KA+  D   
Sbjct: 336 IRGCLETANFSVMINGRPRGKFRASRGLRQGDPLSPFLFTLVMDVLSRIMEKAQDADEFH 395

Query: 305 GLHVGGSHALSITHLQFADDTILFSSPNEAHLDNLFKSIKLFEEAPGLNINCFKTEFMGI 364
           GL  G    + I+HLQFADDTI F    E + +NL + ++LF    G+ IN  K   +GI
Sbjct: 396 GLSPGNG-MVEISHLQFADDTIFFIEDKEEYWNNLLQILELFCFVSGMTINKSKCSLVGI 455

Query: 365 GLDPQILYSLADRYGCKIGGWPNTYLGLPLNGKPKSLTFWASVLEKIEKRLHSWGSQHLP 424
            LD  ++  +A  +GC +G WP  YLGLPL G P+++ FW  V+EK+E RL  W    L 
Sbjct: 456 NLDDGMVNEMAGAWGCDVGVWPMLYLGLPLGGNPRAIKFWDPVVEKVENRLQKWKRACLS 515

Query: 425 KGCRLTLIQATL 436
           KG RLT+IQA L
Sbjct: 516 KGGRLTMIQAVL 525

[Close]

BLAST of Lag0017270.1 vs. TAIR 10
Match: AT1G43760.1 (DNAse I-like superfamily protein )

HSP 1 Score: 78.2 bits (191), Expect = 1.8e-14
Identity = 37/101 (36.63%), Postives = 60/101 (59.41%), Query Frame = 0


Query: 58  SDSLSASLEVPFTEKEVHRAVNDLGSNKSPGLDGFTAEFFKKSWNILKKDIMGVFNDFFK 117
           +D+L++ L    ++KE+  AV  +  NK+PG D FTAEFF +SW ++K   +    +FF+
Sbjct: 520 NDTLASRLSALPSDKEITAAVFAMPRNKAPGPDSFTAEFFWESWFVVKDSTIAAVKEFFR 579

Query: 118 SATINANLNETYICLIPKKIGAKSVGDYRPISLTSCLYKIV 159
           +  +    N T I LIPK  G   +  +RP+S  + +YKI+
Sbjct: 580 TGHLLKRFNATAITLIPKVTGVDQLSMFRPVSCCTVVYKII 620

[Close]

BLAST of Lag0017270.1 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 72.0 bits (175), Expect = 1.3e-12
Identity = 35/69 (50.72%), Postives = 48/69 (69.57%), Query Frame = 0


Query: 256 IINGKPRGKFDASRGLRQGDPLSPFLFIMVVDCLSRMLIKAEQQDLIKGLHVGGSHALSI 315
           IING P+G    SRGLRQGDPLSP+LFI+  + LS +  +A++Q  + G+ V  +++  I
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRV-SNNSPRI 72

Query: 316 THLQFADDT 325
            HL FADDT
Sbjct: 73  NHLLFADDT 80

[Close]

BLAST of Lag0017270.1 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 50.1 bits (118), Expect = 5.4e-06
Identity = 28/79 (35.44%), Postives = 45/79 (56.96%), Query Frame = 0


Query: 164 ERLKKVLPHTIIEYQSVFVADRQILDASLVANELIDEWQRKK-EKG-VCIKLDIEKAFDM 223
           ERLK ++ + I   Q+ F+  R   D  +   E +   +RKK  KG + +KLD+EKA+D 
Sbjct: 3   ERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSMRRKKGVKGWMLLKLDLEKAYDR 62

Query: 224 VDWEFLDEILRVKGFGYTW 241
           + W++L++ L   GF   W
Sbjct: 63  IRWDYLEDTLISAGFPEVW 81

The following BLAST results are available for this feature:

BLAST of Lag0017270.1 vs. NCBI nr


Match Name	E-value	Identity	Description
BBN69746.1	7.0e-117	49.54	VIRB2-interacting protein 2 [Prunus dulcis]	[more]
VVA21938.1	7.0e-117	49.54	Hypothetical predicted protein, partial [Prunus dulcis]	[more]
VVA13439.1	7.0e-117	49.54	Hypothetical predicted protein, partial [Prunus dulcis]	[more]
VVA41200.1	7.0e-117	49.54	PREDICTED: RNA-directed DNA polymerase, partial [Prunus dulcis]	[more]
BBG98819.1	7.0e-117	49.54	VIRB2-interacting protein 2 [Prunus dulcis] >BBH09158.1 VIRB2-interacting protei...	[more]

BLAST of Lag0017270.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O00370	4.8e-36	28.34	LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1	[more]
P14381	7.2e-32	33.11	Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV...	[more]
P11369	8.0e-31	26.82	LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE...	[more]
P08548	4.0e-30	28.65	LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1	[more]
Q03274	1.5e-13	28.07	Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fra...	[more]

BLAST of Lag0017270.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A4Y1R3V4	3.4e-117	49.54	VIRB2-interacting protein 2 OS=Prunus dulcis OX=3755 GN=Prudu_008314 PE=4 SV=1	[more]
A0A5E4GN72	3.4e-117	49.54	PREDICTED: RNA-directed DNA polymerase (Fragment) OS=Prunus dulcis OX=3755 GN=AL...	[more]
A0A5H2Y6K0	3.4e-117	49.54	VIRB2-interacting protein 2 OS=Prunus dulcis OX=3755 GN=Prudu_1151S000200 PE=4 S...	[more]
A0A5E4F859	3.4e-117	49.54	Reverse transcriptase domain-containing protein (Fragment) OS=Prunus dulcis OX=3...	[more]
A0A5E4EEP2	3.4e-117	49.54	Reverse transcriptase domain-containing protein (Fragment) OS=Prunus dulcis OX=3...	[more]

BLAST of Lag0017270.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G43760.1	1.8e-14	36.63	DNAse I-like superfamily protein	[more]
ATMG01250.1	1.3e-12	50.72	RNA-directed DNA polymerase (reverse transcriptase)	[more]
AT4G20520.1	5.4e-06	35.44	RNA binding;RNA-directed DNA polymerases	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 133..391 e-value: 4.7E-42 score: 144.0
IPR000477	Reverse transcriptase domain	PROSITE	PS50878	RT_POL	coord: 114..393 score: 18.817633
None	No IPR available	PANTHER	PTHR33116:SF38	OS01G0158850 PROTEIN	coord: 4..434
None	No IPR available	PANTHER	PTHR33116	REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED	coord: 4..434
None	No IPR available	CDD	cd01650	RT_nLTR_like	coord: 127..393 e-value: 1.04266E-53 score: 176.713
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 32..371

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0017270	Lag0017270	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0017270.1.exon1	Lag0017270.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0017270.1	cds.Lag0017270.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0017270.1	Lag0017270.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090304	nucleic acid metabolic process
molecular_function	GO:0016740	transferase activity