Lag0009349.1 (mRNA) Sponge gourd (AG‐4) v1

BLAST of Lag0009349.1 vs. NCBI nr
Match: XP_038901121.1 (rop guanine nucleotide exchange factor 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 541/572 (94.58%), Postives = 556/572 (97.20%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGAASSSMASSPHPGSGN 60
           MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEG ASSSMASSPHP SGN
Sbjct: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEG-ASSSMASSPHPVSGN 60

Query: 61  FCFPPPLSLPILGGKDVFLWDDKAKKRDTDLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120
           FCFPPP+SLP+L GKDVFLWDDK+KKRD DLSEVEMMKERFAKLLLGEDMSGGGKGVCTA
Sbjct: 61  FCFPPPVSLPVLAGKDVFLWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120

Query: 121 LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180
           LAISNAITNLSA+VFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG
Sbjct: 121 LAISNAITNLSASVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180

Query: 181 TYEVMMSKPRSDLHMNLPALKKLDAMLLGMLDGFCHTEFWYVDRGIVVGDLNDCNDFLPG 240
           TYEVMMSK RSDLHMNLPALKKLDA+LLG+L GFCHTEFWYVDRGIV+GDLNDCNDFLPG
Sbjct: 181 TYEVMMSKLRSDLHMNLPALKKLDALLLGILGGFCHTEFWYVDRGIVLGDLNDCNDFLPG 240

Query: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPT 300
           GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPT
Sbjct: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPT 300

Query: 301 AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEVANRIEASVHIWRQ 360
           AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLE+ANRIEAS+HIWRQ
Sbjct: 301 AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEIANRIEASIHIWRQ 360

Query: 361 KDHKRSGHRSKGRRPTWSGKVKGIVADPGKSHNLARRAETLLDSLRLRFPGLPQTALDMA 420
           KDHKRSGHRSKGRRPTWSGKVKGIVADP KS+NLARRAETLLDSLRLRFPGLPQTALDMA
Sbjct: 361 KDHKRSGHRSKGRRPTWSGKVKGIVADPRKSNNLARRAETLLDSLRLRFPGLPQTALDMA 420

Query: 421 KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGGL 480
           KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGGL
Sbjct: 421 KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGGL 480

Query: 481 PIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRCNAKESQVVLRP 540
           PIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKR N    + VLR 
Sbjct: 481 PIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRSN-NMKETVLRQ 540

Query: 541 DNKHSQADKEKIWSYAGNLSGRRISGVTPERD 573
           D K SQ++KE+IWSYAGNLSGRR+SGVTPERD
Sbjct: 541 D-KQSQSEKERIWSYAGNLSGRRVSGVTPERD 569

BLAST of Lag0009349.1 vs. NCBI nr
Match: XP_038901120.1 (rop guanine nucleotide exchange factor 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 541/573 (94.42%), Postives = 556/573 (97.03%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGAASSSMASSPHPGSGN 60
           MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEG ASSSMASSPHP SGN
Sbjct: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEG-ASSSMASSPHPVSGN 60

Query: 61  FCFPPPLSLPILGGKDVFLWDDKAKKRDTDLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120
           FCFPPP+SLP+L GKDVFLWDDK+KKRD DLSEVEMMKERFAKLLLGEDMSGGGKGVCTA
Sbjct: 61  FCFPPPVSLPVLAGKDVFLWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120

Query: 121 LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180
           LAISNAITNLSA+VFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG
Sbjct: 121 LAISNAITNLSASVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180

Query: 181 TYEVMMSKPRSDLHMNLPALKKLDAMLLGMLDGFCHTEFWYVDRGIVVGDLNDCNDFLPG 240
           TYEVMMSK RSDLHMNLPALKKLDA+LLG+L GFCHTEFWYVDRGIV+GDLNDCNDFLPG
Sbjct: 181 TYEVMMSKLRSDLHMNLPALKKLDALLLGILGGFCHTEFWYVDRGIVLGDLNDCNDFLPG 240

Query: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPT 300
           GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPT
Sbjct: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPT 300

Query: 301 AYMETLPK-SGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEVANRIEASVHIWR 360
           AYMETLPK SGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLE+ANRIEAS+HIWR
Sbjct: 301 AYMETLPKQSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEIANRIEASIHIWR 360

Query: 361 QKDHKRSGHRSKGRRPTWSGKVKGIVADPGKSHNLARRAETLLDSLRLRFPGLPQTALDM 420
           QKDHKRSGHRSKGRRPTWSGKVKGIVADP KS+NLARRAETLLDSLRLRFPGLPQTALDM
Sbjct: 361 QKDHKRSGHRSKGRRPTWSGKVKGIVADPRKSNNLARRAETLLDSLRLRFPGLPQTALDM 420

Query: 421 AKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGG 480
           AKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGG
Sbjct: 421 AKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGG 480

Query: 481 LPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRCNAKESQVVLR 540
           LPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKR N    + VLR
Sbjct: 481 LPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRSN-NMKETVLR 540

Query: 541 PDNKHSQADKEKIWSYAGNLSGRRISGVTPERD 573
            D K SQ++KE+IWSYAGNLSGRR+SGVTPERD
Sbjct: 541 QD-KQSQSEKERIWSYAGNLSGRRVSGVTPERD 570

BLAST of Lag0009349.1 vs. NCBI nr
Match: XP_004139953.1 (rop guanine nucleotide exchange factor 1 [Cucumis sativus] >KGN46713.1 hypothetical protein Csa_020632 [Cucumis sativus])

HSP 1 Score: 1089.7 bits (2817), Expect = 0.0e+00
Identity = 532/572 (93.01%), Postives = 553/572 (96.68%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGAASSSMASSPHPGSGN 60
           MGSVSSDDGSDQLS+RCGSYSLSADVSESESCSSFSCRRFDGEG ASSSMASSPHP S N
Sbjct: 1   MGSVSSDDGSDQLSDRCGSYSLSADVSESESCSSFSCRRFDGEG-ASSSMASSPHPVSAN 60

Query: 61  FCFPPPLSLPILGGKDVFLWDDKAKKRDTDLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120
           FCFPPP+SLP+LGGKDVF+WDDK+KKR+ DLSEVEMMKERFAKLLLGEDMSGGGKGVCTA
Sbjct: 61  FCFPPPVSLPVLGGKDVFVWDDKSKKREADLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120

Query: 121 LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180
           LAISNAITNLSA+VFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG
Sbjct: 121 LAISNAITNLSASVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180

Query: 181 TYEVMMSKPRSDLHMNLPALKKLDAMLLGMLDGFCHTEFWYVDRGIVVGDLNDCNDFLPG 240
           TYEVMMSKPRSDLHMNLPALKKLDA+LLG+L GFCHTEFWYVDRGIV+GDLNDCNDFLPG
Sbjct: 181 TYEVMMSKPRSDLHMNLPALKKLDALLLGILGGFCHTEFWYVDRGIVLGDLNDCNDFLPG 240

Query: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPT 300
           GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVP 
Sbjct: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPA 300

Query: 301 AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEVANRIEASVHIWRQ 360
           AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLE+ANRIEAS+HIWRQ
Sbjct: 301 AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEIANRIEASIHIWRQ 360

Query: 361 KDHKRSGHRSKGRRPTWSGKVKGIVADPGKSHNLARRAETLLDSLRLRFPGLPQTALDMA 420
           KDHKRSGHR+KGRRPTWSGKVKGIV DP KS+NLARRAETLLDSLRLRFPGLPQTALDMA
Sbjct: 361 KDHKRSGHRNKGRRPTWSGKVKGIVGDPRKSNNLARRAETLLDSLRLRFPGLPQTALDMA 420

Query: 421 KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGGL 480
           KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCV+AESISLFNRGLGGL
Sbjct: 421 KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVKAESISLFNRGLGGL 480

Query: 481 PIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRCNAKESQVVLRP 540
           PIQKRMSPSPFSIH SPFASPFATPTFCSSTP+GGSPGRLSP PTVKR N K+   VL  
Sbjct: 481 PIQKRMSPSPFSIHQSPFASPFATPTFCSSTPVGGSPGRLSPPPTVKRSNTKKETAVLSK 540

Query: 541 DNKHSQADKEKIWSYAGNLSGRRISGVTPERD 573
           D K SQ++K+KIWSYAGNLSGRR+SGVTPERD
Sbjct: 541 D-KQSQSEKDKIWSYAGNLSGRRVSGVTPERD 570

BLAST of Lag0009349.1 vs. NCBI nr
Match: XP_008448331.1 (PREDICTED: rop guanine nucleotide exchange factor 1 [Cucumis melo] >KAA0061984.1 rop guanine nucleotide exchange factor 1 [Cucumis melo var. makuwa] >TYK30532.1 rop guanine nucleotide exchange factor 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 531/572 (92.83%), Postives = 551/572 (96.33%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGAASSSMASSPHPGSGN 60
           M S+SSDDGSDQLS+RCGSYSLSADVSESESCSSFSCRRFDGEG ASSSMASSPHP S N
Sbjct: 1   MSSMSSDDGSDQLSDRCGSYSLSADVSESESCSSFSCRRFDGEG-ASSSMASSPHPVSAN 60

Query: 61  FCFPPPLSLPILGGKDVFLWDDKAKKRDTDLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120
           FCFPPP+SLP+LGGKDVF+WDDK+KKR+ DLSEVEMMKERFAKLLLGEDMSGGGKGVCTA
Sbjct: 61  FCFPPPVSLPLLGGKDVFVWDDKSKKREADLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120

Query: 121 LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180
           LAISNAITNLSA+VFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG
Sbjct: 121 LAISNAITNLSASVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180

Query: 181 TYEVMMSKPRSDLHMNLPALKKLDAMLLGMLDGFCHTEFWYVDRGIVVGDLNDCNDFLPG 240
           TYEVMMSKPRSDLHMNLPALKKLDA+LLG+L GFCHTEFWYVDRGIV+GDLNDCNDFLPG
Sbjct: 181 TYEVMMSKPRSDLHMNLPALKKLDALLLGILGGFCHTEFWYVDRGIVLGDLNDCNDFLPG 240

Query: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPT 300
           GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVL EMEVP 
Sbjct: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLVEMEVPA 300

Query: 301 AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEVANRIEASVHIWRQ 360
           AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLE+ANRIEAS+HIWRQ
Sbjct: 301 AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEIANRIEASIHIWRQ 360

Query: 361 KDHKRSGHRSKGRRPTWSGKVKGIVADPGKSHNLARRAETLLDSLRLRFPGLPQTALDMA 420
           KDHKRS HR+KGRRPTWSGKVKGIV DP KS+NLARRAETLLDSLRLRFPGLPQTALDMA
Sbjct: 361 KDHKRSAHRNKGRRPTWSGKVKGIVGDPRKSNNLARRAETLLDSLRLRFPGLPQTALDMA 420

Query: 421 KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGGL 480
           KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGGL
Sbjct: 421 KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGGL 480

Query: 481 PIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRCNAKESQVVLRP 540
           PIQKRMSPSPFSIH SPFASPFATPTFCSSTPIGGSPGRLSPSPTVKR N K+   VL  
Sbjct: 481 PIQKRMSPSPFSIHQSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRSNTKKETAVLSK 540

Query: 541 DNKHSQADKEKIWSYAGNLSGRRISGVTPERD 573
           D K SQ++K+KIWSYAGNLSGRR+SGVTPERD
Sbjct: 541 D-KQSQSEKDKIWSYAGNLSGRRVSGVTPERD 570

BLAST of Lag0009349.1 vs. NCBI nr
Match: XP_022947474.1 (rop guanine nucleotide exchange factor 1-like [Cucurbita moschata] >XP_022947475.1 rop guanine nucleotide exchange factor 1-like [Cucurbita moschata] >KAG6604956.1 Rop guanine nucleotide exchange factor 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034990.1 Rop guanine nucleotide exchange factor 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 535/574 (93.21%), Postives = 550/574 (95.82%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGAASSSMASSPHPGSGN 60
           M SVSSDDGSDQLS+RCGSYSLSADVSESESCSSFSCRRFDGEG ASSSMASSPHP SGN
Sbjct: 1   MASVSSDDGSDQLSDRCGSYSLSADVSESESCSSFSCRRFDGEG-ASSSMASSPHPVSGN 60

Query: 61  FCFPPPLSLPILGGKDVFLWDDKAKKRDTDLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120
           FCFPPP+SLP++GGKDV +WD K KKRD DLSEVEMMKERFAKLLLGEDMSGGGKGVCTA
Sbjct: 61  FCFPPPVSLPVIGGKDVLVWDGKPKKRDDDLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120

Query: 121 LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180
           LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG
Sbjct: 121 LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180

Query: 181 TYEVMMSKPRSDLHMNLPALKKLDAMLLGMLDGFCHTEFWYVDRGIVVGDLNDC-NDFLP 240
           TYEVMMSKPRSDLHMNLPALKKLDAMLL MLDGFCHTEFWYVDRGIV+GDLNDC NDFLP
Sbjct: 181 TYEVMMSKPRSDLHMNLPALKKLDAMLLAMLDGFCHTEFWYVDRGIVLGDLNDCNNDFLP 240

Query: 241 GGRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVP 300
           GGRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVP
Sbjct: 241 GGRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVP 300

Query: 301 TAYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEVANRIEASVHIWR 360
           TAYMETLP+SGKACLGDI+YRY+TADHFSPECLLDCLDLSSEHHTLE+ANRIEASVHIWR
Sbjct: 301 TAYMETLPRSGKACLGDIVYRYITADHFSPECLLDCLDLSSEHHTLEIANRIEASVHIWR 360

Query: 361 QKDHKRSGHRSKGRRPTWSGKVKGIVADPGKSHNLARRAETLLDSLRLRFPGLPQTALDM 420
            KD KRSGHRS GRRPTWSGKVKG+VA PGKSHN+ARRAETLL+SLRLRFPGLPQTALDM
Sbjct: 361 LKDQKRSGHRSTGRRPTWSGKVKGMVAVPGKSHNMARRAETLLESLRLRFPGLPQTALDM 420

Query: 421 AKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGG 480
           AKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIK+CVEAESISLFNRGLGG
Sbjct: 421 AKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKQCVEAESISLFNRGLGG 480

Query: 481 LPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRCNAKESQVVLR 540
           LPIQKRMSPSPFSIHHSPFASPF TPTFCSSTPI GSP RLSPSPTVKR N KE+  VLR
Sbjct: 481 LPIQKRMSPSPFSIHHSPFASPFGTPTFCSSTPIEGSPERLSPSPTVKRSNVKETPDVLR 540

Query: 541 PDNKHSQ-ADKEKIWSYAGNLSGRRISGVTPERD 573
           PD KHSQ A  EKIWSYAGNLSGRRISGVTPERD
Sbjct: 541 PD-KHSQAAGNEKIWSYAGNLSGRRISGVTPERD 572

BLAST of Lag0009349.1 vs. ExPASy Swiss-Prot
Match: Q93ZY2 (Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF1 PE=1 SV=2)

HSP 1 Score: 741.9 bits (1914), Expect = 5.5e-213
Identity = 388/574 (67.60%), Postives = 454/574 (79.09%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGAASSSMASSPHPGSG- 60
           MGS+SS++  +  SERCGSYS SAD+SESES SSFSC RFDGEG ASSS+ SSP   +G 
Sbjct: 1   MGSLSSEEDDEVSSERCGSYSPSADISESESSSSFSCHRFDGEG-ASSSIPSSPRVVAGR 60

Query: 61  NFCFPPPLSLPILGGKDVFLWDDKAKKRDTDLSEVEMMKERFAKLLLGEDMSGGGKGVCT 120
            F FP P+ LP++GGKDV +WDD  K+ D DLSE+EMMKERFAKLLLGEDMSGGGKGVCT
Sbjct: 61  GFYFPAPVMLPVIGGKDV-VWDD--KQPDNDLSEIEMMKERFAKLLLGEDMSGGGKGVCT 120

Query: 121 ALAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGG 180
           ALAISNAITNLSATVFGELWRLEPLAPQK+AMW RE+EWLLCV DSIVEL+P++Q FPGG
Sbjct: 121 ALAISNAITNLSATVFGELWRLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFPGG 180

Query: 181 GTYEVMMSKPRSDLHMNLPALKKLDAMLLGMLDGFCHTEFWYVDRGIVVGDLNDCNDFLP 240
           GTYE+M ++PRSDL+ NLPALKKLDAML+ MLD F  TEFWY DRGIV+G   DC+    
Sbjct: 181 GTYEIMETRPRSDLYANLPALKKLDAMLIDMLDAFSDTEFWYTDRGIVLG---DCDKDSY 240

Query: 241 GGRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVP 300
               S+RQEDKWWLPCPKVP +GLSE+ARK+LQQCRD  NQILKAA+AINS VLAEME+P
Sbjct: 241 NSPASVRQEDKWWLPCPKVPPNGLSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIP 300

Query: 301 TAYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEVANRIEASVHIWR 360
             Y+ETLPKSGK CLG+IIY+Y+TA+ FSPECLLDCLDLSSEH TLE+ANRIEA+VH+WR
Sbjct: 301 DPYLETLPKSGKECLGEIIYQYLTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWR 360

Query: 361 QKDHKRSGHRSKGRRPTWSGKVKGIVADPGKSHNLARRAETLLDSLRLRFPGLPQTALDM 420
           QK+ +R   ++K +  +W GKVKG+V D  ++  L +RAETLL SLR+RFPGLPQT LDM
Sbjct: 361 QKNGRRHKKQAKLKLSSWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDM 420

Query: 421 AKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVE-AESISLFNRGLG 480
            KIQYNKDVGQSILESYSRVMES+AFNI ARIDDVLYVDDA++R +   ES+SLF+  + 
Sbjct: 421 NKIQYNKDVGQSILESYSRVMESMAFNITARIDDVLYVDDAMRRSISVTESLSLFS--IN 480

Query: 481 GLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRCNAKESQVVL 540
           GL  QK      FS+  SP  SPFATP    ++    SP R  P  +VKR   +E  +V 
Sbjct: 481 GLNPQK-----AFSVQSSPHGSPFATPALSVAS---RSPRRAPPLYSVKRNGTREKGIV- 540

Query: 541 RPDNKHSQADKEKIWSYAGNLSGRRISGVTPERD 573
                    + EK WSYAGNLS RR++GVTPERD
Sbjct: 541 --------GETEKAWSYAGNLSSRRVTGVTPERD 548

BLAST of Lag0009349.1 vs. ExPASy Swiss-Prot
Match: Q9LZN0 (Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF7 PE=1 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 6.4e-137
Identity = 269/507 (53.06%), Postives = 347/507 (68.44%), Query Frame = 0

Query: 28  ESESCSSFSCRRFDGEGAASSSMASSPHPGSGNFCFPPPLSLPILGGKDVFLWDDKAKKR 87
           ES  CSS + R+ + E + S +   +  P S  +      S+  + G      + K  + 
Sbjct: 17  ESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRW------SVKNIDG------EKKKIRS 76

Query: 88  DTDLSEVEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQ 147
           D+ +SEVEMMKERF+KLLLGEDMSG G GVCTALAISNAITNL AT+FG+LWRLEPL  +
Sbjct: 77  DSRVSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEPLPTE 136

Query: 148 KRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGTYEVMMSKPRSDLHMNLPALKKLDAML 207
           K+ MW REMEWLLCV D IVE+ PT Q FP G   E+M  +PRSDL++NLPAL+KLD ML
Sbjct: 137 KKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDNML 196

Query: 208 LGMLDGFCHTEFWYVDRGIVVGDLNDCNDFLPGGRPSI-RQEDKWWLPCPKVPADGLSED 267
           L +LD F  TEFWYVD+GI+  +     D     R S  RQEDKWWLP P+V   GL E+
Sbjct: 197 LEILDSFEETEFWYVDQGIMAHE--SAADGSSSFRKSFQRQEDKWWLPVPRVSPGGLQEN 256

Query: 268 ARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPTAYMETLPKSGKACLGDIIYRYMTADH 327
           +RK+LQ  RDCTNQILKAAMAINS  LA+ME+P +Y+E+LP+ G++CLGD+IYRY+++D 
Sbjct: 257 SRKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYISSDQ 316

Query: 328 FSPECLLDCLDLSSEHHTLEVANRIEASVHIWRQKDHKRSGHRSKGRRPTWSGKVKGIVA 387
           FSPECLLDCLDLSSEH  +E+ANR+E+S+++W    HKR+  +      T    VK ++ 
Sbjct: 317 FSPECLLDCLDLSSEHQAIEIANRVESSIYLW----HKRTNSKPATNTKTSWEMVKELMV 376

Query: 388 DPGKSHNLARRAETLLDSLRLRFPGLPQTALDMAKIQYNKDVGQSILESYSRVMESLAFN 447
           D  K   +A RAE+LL SL+ RFPGLPQTALDM+KIQYNKD+G+SILESYSRV+ESLAFN
Sbjct: 377 DADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLESLAFN 436

Query: 448 IMARIDDVLYVDDAIKRCVEAESISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPT 507
           I+ARIDD+L+VDD  +   +    +L N G          +P   ++  S + +P  +P+
Sbjct: 437 IVARIDDLLFVDDLTRHSSDQIPTTLGNNG--------NDAPKSIAVPVSNYTTPSYSPS 496

Query: 508 ---FCSSTPIGGSPGR--LSPSPTVKR 529
                SS  +  SP R  +  S +VKR
Sbjct: 497 KQELRSSITVPPSPSRFKIPHSSSVKR 497

BLAST of Lag0009349.1 vs. ExPASy Swiss-Prot
Match: F4K295 (Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF5 PE=2 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 1.4e-128
Identity = 265/530 (50.00%), Postives = 352/530 (66.42%), Query Frame = 0

Query: 21  SLSADVSESESCSSFSCRRFDGEGAASSSMASSPHPGSGNFCFPPP---LSLPI------ 80
           S+   ++ES+  SS S   +  E ++++++ASS  P       PPP   L  PI      
Sbjct: 21  SMEDILTESKERSSTSGASY--ESSSTTTVASSSPP-------PPPSQILGWPIRKASFR 80

Query: 81  --------LGGKDVFLWDDKA-KKRDTDLSEVEMMKERFAKLLLGEDMSGGGKGVCTALA 140
                      K + L DD   K ++ + ++VEMMKERFAKLLLGEDMSG GKGVCTALA
Sbjct: 81  KNSKESVNFDHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGEDMSGSGKGVCTALA 140

Query: 141 ISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGTY 200
           ISNAITNL AT+FG+LWRLEPL+ +K+ MW REMEW+L V D IVEL P+ Q +P G  +
Sbjct: 141 ISNAITNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELTPSTQTYPDGNKF 200

Query: 201 EVMMSKPRSDLHMNLPALKKLDAMLLGMLDGFCHTEFWYVDRGIVVGDLNDCNDFLPGGR 260
           EVM  +PR DL +NLPAL+KLD MLL +L  F  TEFWYVD+GIV  + +    F    R
Sbjct: 201 EVMTCRPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASENDGSASF---RR 260

Query: 261 PSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPTAY 320
              RQE+KWWLP P++  +GL+E+AR  L   R+C  QILKAAMAINS  L EM+VP +Y
Sbjct: 261 KIQRQEEKWWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLALTEMDVPKSY 320

Query: 321 METLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEVANRIEASVHIWRQKD 380
           +ETLPK+G++CLGD+IYRY+T+D FS E LLDCLDLSSEH  L++ANR+EAS+++WR++ 
Sbjct: 321 LETLPKNGRSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEASIYVWRRRV 380

Query: 381 HKRSG--HRSKGRRP--TWSGKVKGIVADPGKSHNLARRAETLLDSLRLRFPGLPQTALD 440
             + G  + +    P  TW   VK ++A   K   L  R+ETLL  L+ RFP L QT+LD
Sbjct: 381 QTKLGVNNNTSSTTPKLTWE-MVKELMAAGDKRGLLVERSETLLRCLKQRFPSLTQTSLD 440

Query: 441 MAKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLG 500
           ++KIQ+NKD+G+SILESYSR +ESLA NI+ARIDD+LYVDD  K+  +   +S  +  + 
Sbjct: 441 ISKIQWNKDIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNNLLS--SPAVS 500

Query: 501 GLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKR 529
            +   K++ P P+ I  S   +P+ T    S+TP G SP  +SP    +R
Sbjct: 501 SIIAHKKVVPLPYLI--SASGTPYRTS--FSTTP-GFSPSMISPKKGERR 530

BLAST of Lag0009349.1 vs. ExPASy Swiss-Prot
Match: Q9M056 (Rop guanine nucleotide exchange factor 6 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF6 PE=2 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 3.2e-120
Identity = 238/462 (51.52%), Postives = 310/462 (67.10%), Query Frame = 0

Query: 85  KKRDTDLSEVEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPL 144
           K+  T+ SE+E++KER AKLLLGEDMSG G+GVC ALAISNAITNL A + G+ WRLEP+
Sbjct: 99  KRVGTETSEIELLKERMAKLLLGEDMSGSGEGVCPALAISNAITNLYAAILGQQWRLEPI 158

Query: 145 APQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGTYEVMMSKPRSDLHMNLPALKKLD 204
             +K+ MW RE+E LL V D IVELVP+ Q FP G   EVM  +PRSDL   LPAL+KLD
Sbjct: 159 PSEKKLMWRREIEVLLSVSDHIVELVPSFQNFPNGNKIEVMNCRPRSDLFTCLPALRKLD 218

Query: 205 AMLLGMLDGFCHTEFWYVDRGIVVGDLNDCNDFLPGGRPSIRQEDKWWLPCPKVPADGLS 264
            ML+ +LD F  TEFWYVD+GIV  +    N F   G       DKWWLP P+VP+DGL+
Sbjct: 219 NMLIEILDSFGETEFWYVDQGIVAAESARSNSFREDG-------DKWWLPLPRVPSDGLT 278

Query: 265 EDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPTAYMETLPKSGKACLGDIIYRYMTA 324
           E  RK+L   R+ TNQILKA M+INS  LAEMEVP +Y+E LPK+G++CLGD +YR +T+
Sbjct: 279 EQTRKKLDHTREFTNQILKACMSINSIALAEMEVPQSYLEALPKNGRSCLGDFLYRNITS 338

Query: 325 DHFSPECLLDCLDLSSEHHTLEVANRIEASVHIWRQKDHKR---SGHRSKGRRPTWSGKV 384
           D+FS + LL+ +DLSSE   +E+ANR+EAS+++WR++ H R   S +RS   R  W   V
Sbjct: 339 DNFSADHLLESIDLSSELAVVEMANRVEASMYVWRRRAHSRHLISLYRSTSTR--WGMIV 398

Query: 385 KGIV---ADPGKSHNLARRAETLLDSLRLRFPGLPQTALDMAKIQYNKDVGQSILESYSR 444
           K ++    D  K    A RAE+LL  L+ RFPGL QTALD +KIQYNKDVG+SILESYSR
Sbjct: 399 KEMMMHQTDGDKREIFAERAESLLIRLKQRFPGLRQTALDTSKIQYNKDVGKSILESYSR 458

Query: 445 VMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNR----------GLGGLPIQKRMSP 504
           V+ESLA++I  RI++VL++DD  K   + +  S  ++          G  G   +K  +P
Sbjct: 459 VLESLAYSIGVRIEEVLFMDDISKDDGDGDDDSCSDKLRLLSKEAASGGSGSLREKLSAP 518

Query: 505 SPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRCN 531
           S FS+  S  ++P+ T +F +STP        SP P +   N
Sbjct: 519 SLFSVSFSGTSTPYRTLSFSASTP------SYSPMPLISPIN 545

BLAST of Lag0009349.1 vs. ExPASy Swiss-Prot
Match: A4IJ27 (Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF3 PE=2 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 1.6e-111
Identity = 211/376 (56.12%), Postives = 275/376 (73.14%), Query Frame = 0

Query: 93  EVEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQKRAMW 152
           E+E MKERFAKLLLGEDMSG GKGVCTA+ ISNAITNL ATVFG+  RLEPL  +KRA+W
Sbjct: 104 ELETMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKRALW 163

Query: 153 HREMEWLLCVCDSIVELVPTVQPFPGGGTYEVMMSKPRSDLHMNLPALKKLDAMLLGMLD 212
            REM  LL VCD IVE +P  Q    G T EVM S+PR+D+++NLPAL+KLD+ML+  LD
Sbjct: 164 KREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALD 223

Query: 213 GFCHTEFWYVDRGIVVGDLNDCNDFLPGGRPSI--RQEDKWWLPCPKVPADGLSEDARKR 272
            F +TEFWY + G +   +          R  I  R+E+KWWLP P VP++GLS+ ARK+
Sbjct: 224 SFQNTEFWYAEEGSL--SMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARKQ 283

Query: 273 LQQCRDCTNQILKAAMAINSNVLAEMEVPTAYMETLPKSGKACLGDIIYRYMT-ADHFSP 332
           L+  R+ TNQI KAAMAINS++L+EME+P +YM TLPK GK+ +GD IYRYM+ +  F P
Sbjct: 284 LKNKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFFP 343

Query: 333 ECLLDCLDLSSEHHTLEVANRIEASVHIWRQKDHKRSGHRSKGRRPTWSGKVKGIVAD-- 392
           E LLDCL++SSEH  +++A+R+EAS++ WR+K    +   S      W+  VK +++   
Sbjct: 344 EQLLDCLNISSEHEAVQLADRVEASMYTWRRKSCLSNSKNS------WN-MVKDLMSTTE 403

Query: 393 -PGKSHNLARRAETLLDSLRLRFPGLPQTALDMAKIQYNKDVGQSILESYSRVMESLAFN 452
              K++ +A RAETLL  L+ R+P L QT+LD+ KIQYNKDVG+++LESYSRV+E LAFN
Sbjct: 404 RTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGLAFN 463

Query: 453 IMARIDDVLYVDDAIK 463
           I+A IDDVLYVD  ++
Sbjct: 464 IVAWIDDVLYVDKTMR 470

BLAST of Lag0009349.1 vs. ExPASy TrEMBL
Match: A0A0A0KB11 (PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G126260 PE=4 SV=1)

HSP 1 Score: 1089.7 bits (2817), Expect = 0.0e+00
Identity = 532/572 (93.01%), Postives = 553/572 (96.68%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGAASSSMASSPHPGSGN 60
           MGSVSSDDGSDQLS+RCGSYSLSADVSESESCSSFSCRRFDGEG ASSSMASSPHP S N
Sbjct: 1   MGSVSSDDGSDQLSDRCGSYSLSADVSESESCSSFSCRRFDGEG-ASSSMASSPHPVSAN 60

Query: 61  FCFPPPLSLPILGGKDVFLWDDKAKKRDTDLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120
           FCFPPP+SLP+LGGKDVF+WDDK+KKR+ DLSEVEMMKERFAKLLLGEDMSGGGKGVCTA
Sbjct: 61  FCFPPPVSLPVLGGKDVFVWDDKSKKREADLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120

Query: 121 LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180
           LAISNAITNLSA+VFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG
Sbjct: 121 LAISNAITNLSASVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180

Query: 181 TYEVMMSKPRSDLHMNLPALKKLDAMLLGMLDGFCHTEFWYVDRGIVVGDLNDCNDFLPG 240
           TYEVMMSKPRSDLHMNLPALKKLDA+LLG+L GFCHTEFWYVDRGIV+GDLNDCNDFLPG
Sbjct: 181 TYEVMMSKPRSDLHMNLPALKKLDALLLGILGGFCHTEFWYVDRGIVLGDLNDCNDFLPG 240

Query: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPT 300
           GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVP 
Sbjct: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPA 300

Query: 301 AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEVANRIEASVHIWRQ 360
           AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLE+ANRIEAS+HIWRQ
Sbjct: 301 AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEIANRIEASIHIWRQ 360

Query: 361 KDHKRSGHRSKGRRPTWSGKVKGIVADPGKSHNLARRAETLLDSLRLRFPGLPQTALDMA 420
           KDHKRSGHR+KGRRPTWSGKVKGIV DP KS+NLARRAETLLDSLRLRFPGLPQTALDMA
Sbjct: 361 KDHKRSGHRNKGRRPTWSGKVKGIVGDPRKSNNLARRAETLLDSLRLRFPGLPQTALDMA 420

Query: 421 KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGGL 480
           KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCV+AESISLFNRGLGGL
Sbjct: 421 KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVKAESISLFNRGLGGL 480

Query: 481 PIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRCNAKESQVVLRP 540
           PIQKRMSPSPFSIH SPFASPFATPTFCSSTP+GGSPGRLSP PTVKR N K+   VL  
Sbjct: 481 PIQKRMSPSPFSIHQSPFASPFATPTFCSSTPVGGSPGRLSPPPTVKRSNTKKETAVLSK 540

Query: 541 DNKHSQADKEKIWSYAGNLSGRRISGVTPERD 573
           D K SQ++K+KIWSYAGNLSGRR+SGVTPERD
Sbjct: 541 D-KQSQSEKDKIWSYAGNLSGRRVSGVTPERD 570

BLAST of Lag0009349.1 vs. ExPASy TrEMBL
Match: A0A5D3E3R0 (Rop guanine nucleotide exchange factor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1985G00080 PE=4 SV=1)

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 531/572 (92.83%), Postives = 551/572 (96.33%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGAASSSMASSPHPGSGN 60
           M S+SSDDGSDQLS+RCGSYSLSADVSESESCSSFSCRRFDGEG ASSSMASSPHP S N
Sbjct: 1   MSSMSSDDGSDQLSDRCGSYSLSADVSESESCSSFSCRRFDGEG-ASSSMASSPHPVSAN 60

Query: 61  FCFPPPLSLPILGGKDVFLWDDKAKKRDTDLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120
           FCFPPP+SLP+LGGKDVF+WDDK+KKR+ DLSEVEMMKERFAKLLLGEDMSGGGKGVCTA
Sbjct: 61  FCFPPPVSLPLLGGKDVFVWDDKSKKREADLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120

Query: 121 LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180
           LAISNAITNLSA+VFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG
Sbjct: 121 LAISNAITNLSASVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180

Query: 181 TYEVMMSKPRSDLHMNLPALKKLDAMLLGMLDGFCHTEFWYVDRGIVVGDLNDCNDFLPG 240
           TYEVMMSKPRSDLHMNLPALKKLDA+LLG+L GFCHTEFWYVDRGIV+GDLNDCNDFLPG
Sbjct: 181 TYEVMMSKPRSDLHMNLPALKKLDALLLGILGGFCHTEFWYVDRGIVLGDLNDCNDFLPG 240

Query: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPT 300
           GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVL EMEVP 
Sbjct: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLVEMEVPA 300

Query: 301 AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEVANRIEASVHIWRQ 360
           AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLE+ANRIEAS+HIWRQ
Sbjct: 301 AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEIANRIEASIHIWRQ 360

Query: 361 KDHKRSGHRSKGRRPTWSGKVKGIVADPGKSHNLARRAETLLDSLRLRFPGLPQTALDMA 420
           KDHKRS HR+KGRRPTWSGKVKGIV DP KS+NLARRAETLLDSLRLRFPGLPQTALDMA
Sbjct: 361 KDHKRSAHRNKGRRPTWSGKVKGIVGDPRKSNNLARRAETLLDSLRLRFPGLPQTALDMA 420

Query: 421 KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGGL 480
           KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGGL
Sbjct: 421 KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGGL 480

Query: 481 PIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRCNAKESQVVLRP 540
           PIQKRMSPSPFSIH SPFASPFATPTFCSSTPIGGSPGRLSPSPTVKR N K+   VL  
Sbjct: 481 PIQKRMSPSPFSIHQSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRSNTKKETAVLSK 540

Query: 541 DNKHSQADKEKIWSYAGNLSGRRISGVTPERD 573
           D K SQ++K+KIWSYAGNLSGRR+SGVTPERD
Sbjct: 541 D-KQSQSEKDKIWSYAGNLSGRRVSGVTPERD 570

BLAST of Lag0009349.1 vs. ExPASy TrEMBL
Match: A0A1S3BKB9 (rop guanine nucleotide exchange factor 1 OS=Cucumis melo OX=3656 GN=LOC103490557 PE=4 SV=1)

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 531/572 (92.83%), Postives = 551/572 (96.33%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGAASSSMASSPHPGSGN 60
           M S+SSDDGSDQLS+RCGSYSLSADVSESESCSSFSCRRFDGEG ASSSMASSPHP S N
Sbjct: 1   MSSMSSDDGSDQLSDRCGSYSLSADVSESESCSSFSCRRFDGEG-ASSSMASSPHPVSAN 60

Query: 61  FCFPPPLSLPILGGKDVFLWDDKAKKRDTDLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120
           FCFPPP+SLP+LGGKDVF+WDDK+KKR+ DLSEVEMMKERFAKLLLGEDMSGGGKGVCTA
Sbjct: 61  FCFPPPVSLPLLGGKDVFVWDDKSKKREADLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120

Query: 121 LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180
           LAISNAITNLSA+VFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG
Sbjct: 121 LAISNAITNLSASVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180

Query: 181 TYEVMMSKPRSDLHMNLPALKKLDAMLLGMLDGFCHTEFWYVDRGIVVGDLNDCNDFLPG 240
           TYEVMMSKPRSDLHMNLPALKKLDA+LLG+L GFCHTEFWYVDRGIV+GDLNDCNDFLPG
Sbjct: 181 TYEVMMSKPRSDLHMNLPALKKLDALLLGILGGFCHTEFWYVDRGIVLGDLNDCNDFLPG 240

Query: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPT 300
           GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVL EMEVP 
Sbjct: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLVEMEVPA 300

Query: 301 AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEVANRIEASVHIWRQ 360
           AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLE+ANRIEAS+HIWRQ
Sbjct: 301 AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEIANRIEASIHIWRQ 360

Query: 361 KDHKRSGHRSKGRRPTWSGKVKGIVADPGKSHNLARRAETLLDSLRLRFPGLPQTALDMA 420
           KDHKRS HR+KGRRPTWSGKVKGIV DP KS+NLARRAETLLDSLRLRFPGLPQTALDMA
Sbjct: 361 KDHKRSAHRNKGRRPTWSGKVKGIVGDPRKSNNLARRAETLLDSLRLRFPGLPQTALDMA 420

Query: 421 KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGGL 480
           KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGGL
Sbjct: 421 KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGGL 480

Query: 481 PIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRCNAKESQVVLRP 540
           PIQKRMSPSPFSIH SPFASPFATPTFCSSTPIGGSPGRLSPSPTVKR N K+   VL  
Sbjct: 481 PIQKRMSPSPFSIHQSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRSNTKKETAVLSK 540

Query: 541 DNKHSQADKEKIWSYAGNLSGRRISGVTPERD 573
           D K SQ++K+KIWSYAGNLSGRR+SGVTPERD
Sbjct: 541 D-KQSQSEKDKIWSYAGNLSGRRVSGVTPERD 570

BLAST of Lag0009349.1 vs. ExPASy TrEMBL
Match: A0A6J1G702 (rop guanine nucleotide exchange factor 1-like OS=Cucurbita moschata OX=3662 GN=LOC111451330 PE=4 SV=1)

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 535/574 (93.21%), Postives = 550/574 (95.82%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGAASSSMASSPHPGSGN 60
           M SVSSDDGSDQLS+RCGSYSLSADVSESESCSSFSCRRFDGEG ASSSMASSPHP SGN
Sbjct: 1   MASVSSDDGSDQLSDRCGSYSLSADVSESESCSSFSCRRFDGEG-ASSSMASSPHPVSGN 60

Query: 61  FCFPPPLSLPILGGKDVFLWDDKAKKRDTDLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120
           FCFPPP+SLP++GGKDV +WD K KKRD DLSEVEMMKERFAKLLLGEDMSGGGKGVCTA
Sbjct: 61  FCFPPPVSLPVIGGKDVLVWDGKPKKRDDDLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120

Query: 121 LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180
           LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG
Sbjct: 121 LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180

Query: 181 TYEVMMSKPRSDLHMNLPALKKLDAMLLGMLDGFCHTEFWYVDRGIVVGDLNDC-NDFLP 240
           TYEVMMSKPRSDLHMNLPALKKLDAMLL MLDGFCHTEFWYVDRGIV+GDLNDC NDFLP
Sbjct: 181 TYEVMMSKPRSDLHMNLPALKKLDAMLLAMLDGFCHTEFWYVDRGIVLGDLNDCNNDFLP 240

Query: 241 GGRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVP 300
           GGRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVP
Sbjct: 241 GGRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVP 300

Query: 301 TAYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEVANRIEASVHIWR 360
           TAYMETLP+SGKACLGDI+YRY+TADHFSPECLLDCLDLSSEHHTLE+ANRIEASVHIWR
Sbjct: 301 TAYMETLPRSGKACLGDIVYRYITADHFSPECLLDCLDLSSEHHTLEIANRIEASVHIWR 360

Query: 361 QKDHKRSGHRSKGRRPTWSGKVKGIVADPGKSHNLARRAETLLDSLRLRFPGLPQTALDM 420
            KD KRSGHRS GRRPTWSGKVKG+VA PGKSHN+ARRAETLL+SLRLRFPGLPQTALDM
Sbjct: 361 LKDQKRSGHRSTGRRPTWSGKVKGMVAVPGKSHNMARRAETLLESLRLRFPGLPQTALDM 420

Query: 421 AKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGG 480
           AKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIK+CVEAESISLFNRGLGG
Sbjct: 421 AKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKQCVEAESISLFNRGLGG 480

Query: 481 LPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRCNAKESQVVLR 540
           LPIQKRMSPSPFSIHHSPFASPF TPTFCSSTPI GSP RLSPSPTVKR N KE+  VLR
Sbjct: 481 LPIQKRMSPSPFSIHHSPFASPFGTPTFCSSTPIEGSPERLSPSPTVKRSNVKETPDVLR 540

Query: 541 PDNKHSQ-ADKEKIWSYAGNLSGRRISGVTPERD 573
           PD KHSQ A  EKIWSYAGNLSGRRISGVTPERD
Sbjct: 541 PD-KHSQAAGNEKIWSYAGNLSGRRISGVTPERD 572

BLAST of Lag0009349.1 vs. ExPASy TrEMBL
Match: A0A6J1CFP8 (rop guanine nucleotide exchange factor 1 OS=Momordica charantia OX=3673 GN=LOC111011261 PE=4 SV=1)

HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 533/572 (93.18%), Postives = 546/572 (95.45%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGAASSSMASSPHPGSGN 60
           MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEG ASSSMASSPHP SGN
Sbjct: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEG-ASSSMASSPHPVSGN 60

Query: 61  FCFPPPLSLPILGGKDVFLWDDKAKKRDTDLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120
           FCFPPP+SLP++GGKDVF+WDDK+KKRD DLSEVEMMKERFAKLLLGEDMSGGGKGVCTA
Sbjct: 61  FCFPPPVSLPVIGGKDVFVWDDKSKKRDADLSEVEMMKERFAKLLLGEDMSGGGKGVCTA 120

Query: 121 LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180
           LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG
Sbjct: 121 LAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGG 180

Query: 181 TYEVMMSKPRSDLHMNLPALKKLDAMLLGMLDGFCHTEFWYVDRGIVVGDLNDCNDFLPG 240
           TYEVMMSKPRSDL+MNLPALKKLDAMLL MLDGFCHTEFWYVDRGIVVGDLNDCN FLPG
Sbjct: 181 TYEVMMSKPRSDLYMNLPALKKLDAMLLSMLDGFCHTEFWYVDRGIVVGDLNDCNGFLPG 240

Query: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPT 300
           GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEME+PT
Sbjct: 241 GRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEIPT 300

Query: 301 AYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEVANRIEASVHIWRQ 360
           AY ETLPKSGKACLGDIIYRY+TADHF PECLLDCLDLSSEHHT+EVANRIEASVHIWR 
Sbjct: 301 AYKETLPKSGKACLGDIIYRYITADHFLPECLLDCLDLSSEHHTVEVANRIEASVHIWRA 360

Query: 361 KDHKRSGHRSKGRRPTWSGKVKGIVADPGKSHNLARRAETLLDSLRLRFPGLPQTALDMA 420
           KDHKRSGHRSKGRRPTWSGKVKGIVAD GKSHNLARRAETLL+SLRLRFPGLPQT LDMA
Sbjct: 361 KDHKRSGHRSKGRRPTWSGKVKGIVADSGKSHNLARRAETLLESLRLRFPGLPQTGLDMA 420

Query: 421 KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLGGL 480
           KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAE+ISLFNRGLGGL
Sbjct: 421 KIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAETISLFNRGLGGL 480

Query: 481 PIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRCNAKESQVVLRP 540
           PIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGR   SPTVKR N KE+      
Sbjct: 481 PIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGR--ASPTVKRSNVKEA------ 540

Query: 541 DNKHSQADKEKIWSYAGNLSGRRISGVTPERD 573
                QADKE+IWSYAGNLSGRRISGVTPERD
Sbjct: 541 ----GQADKERIWSYAGNLSGRRISGVTPERD 559

BLAST of Lag0009349.1 vs. TAIR 10
Match: AT4G38430.1 (rho guanyl-nucleotide exchange factor 1 )

HSP 1 Score: 741.9 bits (1914), Expect = 3.9e-214
Identity = 388/574 (67.60%), Postives = 454/574 (79.09%), Query Frame = 0

Query: 1   MGSVSSDDGSDQLSERCGSYSLSADVSESESCSSFSCRRFDGEGAASSSMASSPHPGSG- 60
           MGS+SS++  +  SERCGSYS SAD+SESES SSFSC RFDGEG ASSS+ SSP   +G 
Sbjct: 1   MGSLSSEEDDEVSSERCGSYSPSADISESESSSSFSCHRFDGEG-ASSSIPSSPRVVAGR 60

Query: 61  NFCFPPPLSLPILGGKDVFLWDDKAKKRDTDLSEVEMMKERFAKLLLGEDMSGGGKGVCT 120
            F FP P+ LP++GGKDV +WDD  K+ D DLSE+EMMKERFAKLLLGEDMSGGGKGVCT
Sbjct: 61  GFYFPAPVMLPVIGGKDV-VWDD--KQPDNDLSEIEMMKERFAKLLLGEDMSGGGKGVCT 120

Query: 121 ALAISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGG 180
           ALAISNAITNLSATVFGELWRLEPLAPQK+AMW RE+EWLLCV DSIVEL+P++Q FPGG
Sbjct: 121 ALAISNAITNLSATVFGELWRLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFPGG 180

Query: 181 GTYEVMMSKPRSDLHMNLPALKKLDAMLLGMLDGFCHTEFWYVDRGIVVGDLNDCNDFLP 240
           GTYE+M ++PRSDL+ NLPALKKLDAML+ MLD F  TEFWY DRGIV+G   DC+    
Sbjct: 181 GTYEIMETRPRSDLYANLPALKKLDAMLIDMLDAFSDTEFWYTDRGIVLG---DCDKDSY 240

Query: 241 GGRPSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVP 300
               S+RQEDKWWLPCPKVP +GLSE+ARK+LQQCRD  NQILKAA+AINS VLAEME+P
Sbjct: 241 NSPASVRQEDKWWLPCPKVPPNGLSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIP 300

Query: 301 TAYMETLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEVANRIEASVHIWR 360
             Y+ETLPKSGK CLG+IIY+Y+TA+ FSPECLLDCLDLSSEH TLE+ANRIEA+VH+WR
Sbjct: 301 DPYLETLPKSGKECLGEIIYQYLTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWR 360

Query: 361 QKDHKRSGHRSKGRRPTWSGKVKGIVADPGKSHNLARRAETLLDSLRLRFPGLPQTALDM 420
           QK+ +R   ++K +  +W GKVKG+V D  ++  L +RAETLL SLR+RFPGLPQT LDM
Sbjct: 361 QKNGRRHKKQAKLKLSSWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDM 420

Query: 421 AKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVE-AESISLFNRGLG 480
            KIQYNKDVGQSILESYSRVMES+AFNI ARIDDVLYVDDA++R +   ES+SLF+  + 
Sbjct: 421 NKIQYNKDVGQSILESYSRVMESMAFNITARIDDVLYVDDAMRRSISVTESLSLFS--IN 480

Query: 481 GLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRCNAKESQVVL 540
           GL  QK      FS+  SP  SPFATP    ++    SP R  P  +VKR   +E  +V 
Sbjct: 481 GLNPQK-----AFSVQSSPHGSPFATPALSVAS---RSPRRAPPLYSVKRNGTREKGIV- 540

Query: 541 RPDNKHSQADKEKIWSYAGNLSGRRISGVTPERD 573
                    + EK WSYAGNLS RR++GVTPERD
Sbjct: 541 --------GETEKAWSYAGNLSSRRVTGVTPERD 548

BLAST of Lag0009349.1 vs. TAIR 10
Match: AT5G02010.1 (RHO guanyl-nucleotide exchange factor 7 )

HSP 1 Score: 489.2 bits (1258), Expect = 4.5e-138
Identity = 269/507 (53.06%), Postives = 347/507 (68.44%), Query Frame = 0

Query: 28  ESESCSSFSCRRFDGEGAASSSMASSPHPGSGNFCFPPPLSLPILGGKDVFLWDDKAKKR 87
           ES  CSS + R+ + E + S +   +  P S  +      S+  + G      + K  + 
Sbjct: 17  ESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRW------SVKNIDG------EKKKIRS 76

Query: 88  DTDLSEVEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQ 147
           D+ +SEVEMMKERF+KLLLGEDMSG G GVCTALAISNAITNL AT+FG+LWRLEPL  +
Sbjct: 77  DSRVSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEPLPTE 136

Query: 148 KRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGTYEVMMSKPRSDLHMNLPALKKLDAML 207
           K+ MW REMEWLLCV D IVE+ PT Q FP G   E+M  +PRSDL++NLPAL+KLD ML
Sbjct: 137 KKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDNML 196

Query: 208 LGMLDGFCHTEFWYVDRGIVVGDLNDCNDFLPGGRPSI-RQEDKWWLPCPKVPADGLSED 267
           L +LD F  TEFWYVD+GI+  +     D     R S  RQEDKWWLP P+V   GL E+
Sbjct: 197 LEILDSFEETEFWYVDQGIMAHE--SAADGSSSFRKSFQRQEDKWWLPVPRVSPGGLQEN 256

Query: 268 ARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPTAYMETLPKSGKACLGDIIYRYMTADH 327
           +RK+LQ  RDCTNQILKAAMAINS  LA+ME+P +Y+E+LP+ G++CLGD+IYRY+++D 
Sbjct: 257 SRKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYISSDQ 316

Query: 328 FSPECLLDCLDLSSEHHTLEVANRIEASVHIWRQKDHKRSGHRSKGRRPTWSGKVKGIVA 387
           FSPECLLDCLDLSSEH  +E+ANR+E+S+++W    HKR+  +      T    VK ++ 
Sbjct: 317 FSPECLLDCLDLSSEHQAIEIANRVESSIYLW----HKRTNSKPATNTKTSWEMVKELMV 376

Query: 388 DPGKSHNLARRAETLLDSLRLRFPGLPQTALDMAKIQYNKDVGQSILESYSRVMESLAFN 447
           D  K   +A RAE+LL SL+ RFPGLPQTALDM+KIQYNKD+G+SILESYSRV+ESLAFN
Sbjct: 377 DADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLESLAFN 436

Query: 448 IMARIDDVLYVDDAIKRCVEAESISLFNRGLGGLPIQKRMSPSPFSIHHSPFASPFATPT 507
           I+ARIDD+L+VDD  +   +    +L N G          +P   ++  S + +P  +P+
Sbjct: 437 IVARIDDLLFVDDLTRHSSDQIPTTLGNNG--------NDAPKSIAVPVSNYTTPSYSPS 496

Query: 508 ---FCSSTPIGGSPGR--LSPSPTVKR 529
                SS  +  SP R  +  S +VKR
Sbjct: 497 KQELRSSITVPPSPSRFKIPHSSSVKR 497

BLAST of Lag0009349.1 vs. TAIR 10
Match: AT5G05940.1 (ROP guanine nucleotide exchange factor 5 )

HSP 1 Score: 461.5 bits (1186), Expect = 1.0e-129
Identity = 265/530 (50.00%), Postives = 352/530 (66.42%), Query Frame = 0

Query: 21  SLSADVSESESCSSFSCRRFDGEGAASSSMASSPHPGSGNFCFPPP---LSLPI------ 80
           S+   ++ES+  SS S   +  E ++++++ASS  P       PPP   L  PI      
Sbjct: 21  SMEDILTESKERSSTSGASY--ESSSTTTVASSSPP-------PPPSQILGWPIRKASFR 80

Query: 81  --------LGGKDVFLWDDKA-KKRDTDLSEVEMMKERFAKLLLGEDMSGGGKGVCTALA 140
                      K + L DD   K ++ + ++VEMMKERFAKLLLGEDMSG GKGVCTALA
Sbjct: 81  KNSKESVNFDHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGEDMSGSGKGVCTALA 140

Query: 141 ISNAITNLSATVFGELWRLEPLAPQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGTY 200
           ISNAITNL AT+FG+LWRLEPL+ +K+ MW REMEW+L V D IVEL P+ Q +P G  +
Sbjct: 141 ISNAITNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELTPSTQTYPDGNKF 200

Query: 201 EVMMSKPRSDLHMNLPALKKLDAMLLGMLDGFCHTEFWYVDRGIVVGDLNDCNDFLPGGR 260
           EVM  +PR DL +NLPAL+KLD MLL +L  F  TEFWYVD+GIV  + +    F    R
Sbjct: 201 EVMTCRPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASENDGSASF---RR 260

Query: 261 PSIRQEDKWWLPCPKVPADGLSEDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPTAY 320
              RQE+KWWLP P++  +GL+E+AR  L   R+C  QILKAAMAINS  L EM+VP +Y
Sbjct: 261 KIQRQEEKWWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLALTEMDVPKSY 320

Query: 321 METLPKSGKACLGDIIYRYMTADHFSPECLLDCLDLSSEHHTLEVANRIEASVHIWRQKD 380
           +ETLPK+G++CLGD+IYRY+T+D FS E LLDCLDLSSEH  L++ANR+EAS+++WR++ 
Sbjct: 321 LETLPKNGRSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEASIYVWRRRV 380

Query: 381 HKRSG--HRSKGRRP--TWSGKVKGIVADPGKSHNLARRAETLLDSLRLRFPGLPQTALD 440
             + G  + +    P  TW   VK ++A   K   L  R+ETLL  L+ RFP L QT+LD
Sbjct: 381 QTKLGVNNNTSSTTPKLTWE-MVKELMAAGDKRGLLVERSETLLRCLKQRFPSLTQTSLD 440

Query: 441 MAKIQYNKDVGQSILESYSRVMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNRGLG 500
           ++KIQ+NKD+G+SILESYSR +ESLA NI+ARIDD+LYVDD  K+  +   +S  +  + 
Sbjct: 441 ISKIQWNKDIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNNLLS--SPAVS 500

Query: 501 GLPIQKRMSPSPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKR 529
            +   K++ P P+ I  S   +P+ T    S+TP G SP  +SP    +R
Sbjct: 501 SIIAHKKVVPLPYLI--SASGTPYRTS--FSTTP-GFSPSMISPKKGERR 530

BLAST of Lag0009349.1 vs. TAIR 10
Match: AT3G55660.1 (ROP (rho of plants) guanine nucleotide exchange factor 6 )

HSP 1 Score: 433.7 bits (1114), Expect = 2.3e-121
Identity = 238/462 (51.52%), Postives = 310/462 (67.10%), Query Frame = 0

Query: 85  KKRDTDLSEVEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPL 144
           K+  T+ SE+E++KER AKLLLGEDMSG G+GVC ALAISNAITNL A + G+ WRLEP+
Sbjct: 99  KRVGTETSEIELLKERMAKLLLGEDMSGSGEGVCPALAISNAITNLYAAILGQQWRLEPI 158

Query: 145 APQKRAMWHREMEWLLCVCDSIVELVPTVQPFPGGGTYEVMMSKPRSDLHMNLPALKKLD 204
             +K+ MW RE+E LL V D IVELVP+ Q FP G   EVM  +PRSDL   LPAL+KLD
Sbjct: 159 PSEKKLMWRREIEVLLSVSDHIVELVPSFQNFPNGNKIEVMNCRPRSDLFTCLPALRKLD 218

Query: 205 AMLLGMLDGFCHTEFWYVDRGIVVGDLNDCNDFLPGGRPSIRQEDKWWLPCPKVPADGLS 264
            ML+ +LD F  TEFWYVD+GIV  +    N F   G       DKWWLP P+VP+DGL+
Sbjct: 219 NMLIEILDSFGETEFWYVDQGIVAAESARSNSFREDG-------DKWWLPLPRVPSDGLT 278

Query: 265 EDARKRLQQCRDCTNQILKAAMAINSNVLAEMEVPTAYMETLPKSGKACLGDIIYRYMTA 324
           E  RK+L   R+ TNQILKA M+INS  LAEMEVP +Y+E LPK+G++CLGD +YR +T+
Sbjct: 279 EQTRKKLDHTREFTNQILKACMSINSIALAEMEVPQSYLEALPKNGRSCLGDFLYRNITS 338

Query: 325 DHFSPECLLDCLDLSSEHHTLEVANRIEASVHIWRQKDHKR---SGHRSKGRRPTWSGKV 384
           D+FS + LL+ +DLSSE   +E+ANR+EAS+++WR++ H R   S +RS   R  W   V
Sbjct: 339 DNFSADHLLESIDLSSELAVVEMANRVEASMYVWRRRAHSRHLISLYRSTSTR--WGMIV 398

Query: 385 KGIV---ADPGKSHNLARRAETLLDSLRLRFPGLPQTALDMAKIQYNKDVGQSILESYSR 444
           K ++    D  K    A RAE+LL  L+ RFPGL QTALD +KIQYNKDVG+SILESYSR
Sbjct: 399 KEMMMHQTDGDKREIFAERAESLLIRLKQRFPGLRQTALDTSKIQYNKDVGKSILESYSR 458

Query: 445 VMESLAFNIMARIDDVLYVDDAIKRCVEAESISLFNR----------GLGGLPIQKRMSP 504
           V+ESLA++I  RI++VL++DD  K   + +  S  ++          G  G   +K  +P
Sbjct: 459 VLESLAYSIGVRIEEVLFMDDISKDDGDGDDDSCSDKLRLLSKEAASGGSGSLREKLSAP 518

Query: 505 SPFSIHHSPFASPFATPTFCSSTPIGGSPGRLSPSPTVKRCN 531
           S FS+  S  ++P+ T +F +STP        SP P +   N
Sbjct: 519 SLFSVSFSGTSTPYRTLSFSASTP------SYSPMPLISPIN 545

BLAST of Lag0009349.1 vs. TAIR 10
Match: AT4G00460.2 (RHO guanyl-nucleotide exchange factor 3 )

HSP 1 Score: 404.8 bits (1039), Expect = 1.1e-112
Identity = 211/376 (56.12%), Postives = 275/376 (73.14%), Query Frame = 0

Query: 93  EVEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGELWRLEPLAPQKRAMW 152
           E+E MKERFAKLLLGEDMSG GKGVCTA+ ISNAITNL ATVFG+  RLEPL  +KRA+W
Sbjct: 104 ELETMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKRALW 163

Query: 153 HREMEWLLCVCDSIVELVPTVQPFPGGGTYEVMMSKPRSDLHMNLPALKKLDAMLLGMLD 212
            REM  LL VCD IVE +P  Q    G T EVM S+PR+D+++NLPAL+KLD+ML+  LD
Sbjct: 164 KREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALD 223

Query: 213 GFCHTEFWYVDRGIVVGDLNDCNDFLPGGRPSI--RQEDKWWLPCPKVPADGLSEDARKR 272
            F +TEFWY + G +   +          R  I  R+E+KWWLP P VP++GLS+ ARK+
Sbjct: 224 SFQNTEFWYAEEGSL--SMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARKQ 283

Query: 273 LQQCRDCTNQILKAAMAINSNVLAEMEVPTAYMETLPKSGKACLGDIIYRYMT-ADHFSP 332
           L+  R+ TNQI KAAMAINS++L+EME+P +YM TLPK GK+ +GD IYRYM+ +  F P
Sbjct: 284 LKNKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFFP 343

Query: 333 ECLLDCLDLSSEHHTLEVANRIEASVHIWRQKDHKRSGHRSKGRRPTWSGKVKGIVAD-- 392
           E LLDCL++SSEH  +++A+R+EAS++ WR+K    +   S      W+  VK +++   
Sbjct: 344 EQLLDCLNISSEHEAVQLADRVEASMYTWRRKSCLSNSKNS------WN-MVKDLMSTTE 403

Query: 393 -PGKSHNLARRAETLLDSLRLRFPGLPQTALDMAKIQYNKDVGQSILESYSRVMESLAFN 452
              K++ +A RAETLL  L+ R+P L QT+LD+ KIQYNKDVG+++LESYSRV+E LAFN
Sbjct: 404 RTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGLAFN 463

Query: 453 IMARIDDVLYVDDAIK 463
           I+A IDDVLYVD  ++
Sbjct: 464 IVAWIDDVLYVDKTMR 470