Lag0005423.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0005423.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Eukaryotic translation initiation factor 3 subunit I
Location	chr6: 17405264 .. 17405783 (-)
Sequence length	327
RNA-Seq Expression	Lag0005423.1
Synteny	Lag0005423.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCTTAAGTTCTAAGGATGAAGGTGTTGTGGGGGTAGACTCTTTAATGCTAGATTGTTTAGTTGATGATGTTAAAATATCTTTTGACTCATATCGGTCTCTTGGGTTTGAAGTAGTACTTGGTGGTGGACAAGATGCTTCAGCAGTAATAACTGATCATCGGGTCGGAAAGTTTGAAGCTAAATTCTATCACAAGGTGTAGTGTTCTTCCTAATGACCTTATAATTGAAGTTGAGTTAATTTAGTTTTTTGACATTTTTGTTTTGTGTAATAGATCCTTCAAGTAGATATTGGAGGTGTAAACGACCATTTTTTACCCATTAATGCTCTGGCTTTCAATCCTGATGGCAAGAGGTATGTTTATGGTTTATTGCATTGTTTGCATATGAAAAAAAGCAAACAACACAATCAAAGAAACCATAGTATTGGATGGTGATTGAGATTCTTCATAGATATGGTTTTGTTGTAGTTTTTCAATTAGAGGTGAGTTGGATTATGAAGGTAATTACGATGATTAA

mRNA sequence

ATGCCCTTAAGTTCTAAGGATGAAGGTGTTGTGGGGGTAGACTCTTTAATGCTAGATTGTTTAGTTGATGATGTTAAAATATCTTTTGACTCATATCGGTCTCTTGGGTTTGAAGTAGTACTTGGTGGTGGACAAGATGCTTCAGCAGTAATAACTGATCATCGGGTCGGAAAGTTTGAAGCTAAATTCTATCACAAGATCCTTCAAGTAGATATTGGAGGTGTAAACGACCATTTTTTACCCATTAATGCTCTGGCTTTCAATCCTGATGGCAAGAGTTTTTCAATTAGAGGTGAGTTGGATTATGAAGGTAATTACGATGATTAA

Coding sequence (CDS)

ATGCCCTTAAGTTCTAAGGATGAAGGTGTTGTGGGGGTAGACTCTTTAATGCTAGATTGTTTAGTTGATGATGTTAAAATATCTTTTGACTCATATCGGTCTCTTGGGTTTGAAGTAGTACTTGGTGGTGGACAAGATGCTTCAGCAGTAATAACTGATCATCGGGTCGGAAAGTTTGAAGCTAAATTCTATCACAAGATCCTTCAAGTAGATATTGGAGGTGTAAACGACCATTTTTTACCCATTAATGCTCTGGCTTTCAATCCTGATGGCAAGAGTTTTTCAATTAGAGGTGAGTTGGATTATGAAGGTAATTACGATGATTAA

Protein sequence

MPLSSKDEGVVGVDSLMLDCLVDDVKISFDSYRSLGFEVVLGGGQDASAVITDHRVGKFEAKFYHKILQVDIGGVNDHFLPINALAFNPDGKSFSIRGELDYEGNYDD

Homology

[Close]

BLAST of Lag0005423.1 vs. NCBI nr
Match: KZV44849.1 (eukaryotic translation initiation factor 3 subunit I [Dorcoceras hygrometricum])

HSP 1 Score: 105.1 bits (261), Expect = 3.7e-19
Identity = 53/65 (81.54%), Postives = 54/65 (83.08%), Query Frame = 0


Query: 39  VVLGGGQDASAV-ITDHRVGKFEAKFYHKILQVDIGGVNDHFLPINALAFNPDGKSFSIR 98
           VVLGGGQDASAV  TDHR GKFEAKFYHKILQ +IGGV  HF PINALAFNPDGKSFS  
Sbjct: 247 VVLGGGQDASAVTTTDHRAGKFEAKFYHKILQEEIGGVKGHFGPINALAFNPDGKSFSSG 306

Query: 99  GELDY 103
           GE  Y
Sbjct: 307 GEDGY 311

[Close]

BLAST of Lag0005423.1 vs. NCBI nr
Match: KZV57115.1 (eukaryotic translation initiation factor 3 subunit I [Dorcoceras hygrometricum])

HSP 1 Score: 105.1 bits (261), Expect = 3.7e-19
Identity = 53/65 (81.54%), Postives = 54/65 (83.08%), Query Frame = 0


Query: 39  VVLGGGQDASAV-ITDHRVGKFEAKFYHKILQVDIGGVNDHFLPINALAFNPDGKSFSIR 98
           VVLGGGQDASAV  TDHR GKFEAKFYHKILQ +IGGV  HF PINALAFNPDGKSFS  
Sbjct: 247 VVLGGGQDASAVTTTDHRAGKFEAKFYHKILQEEIGGVKGHFGPINALAFNPDGKSFSSG 306

Query: 99  GELDY 103
           GE  Y
Sbjct: 307 GEDGY 311

[Close]

BLAST of Lag0005423.1 vs. NCBI nr
Match: CAF2143618.1 (unnamed protein product [Brassica napus])

HSP 1 Score: 104.4 bits (259), Expect = 6.4e-19
Identity = 57/87 (65.52%), Postives = 61/87 (70.11%), Query Frame = 0


Query: 17  MLDCLVDDVKISFDSYRSLGFEVVLGGGQDASAV-ITDHRVGKFEAKFYHKILQVDIGGV 76
           +L   V  V ++  S   L   VVLGGGQDASAV  TDHR GKFEAKFY KILQ +IGGV
Sbjct: 513 LLKTYVSGVPVNAVSMSPLLDHVVLGGGQDASAVTTTDHRAGKFEAKFYDKILQEEIGGV 572

Query: 77  NDHFLPINALAFNPDGKSFSIRGELDY 103
             HF PINALAFNPDGKSFS  GE  Y
Sbjct: 573 KGHFGPINALAFNPDGKSFSSGGEDGY 599

[Close]

BLAST of Lag0005423.1 vs. NCBI nr
Match: KAF2597520.1 (hypothetical protein F2Q68_00011701 [Brassica cretica])

HSP 1 Score: 104.4 bits (259), Expect = 6.4e-19
Identity = 57/87 (65.52%), Postives = 61/87 (70.11%), Query Frame = 0


Query: 17  MLDCLVDDVKISFDSYRSLGFEVVLGGGQDASAV-ITDHRVGKFEAKFYHKILQVDIGGV 76
           +L   V  V ++  S   L   VVLGGGQDASAV  TDHR GKFEAKFY KILQ +IGGV
Sbjct: 229 LLKTYVSGVPVNAVSMSPLLDHVVLGGGQDASAVTTTDHRAGKFEAKFYDKILQEEIGGV 288

Query: 77  NDHFLPINALAFNPDGKSFSIRGELDY 103
             HF PINALAFNPDGKSFS  GE  Y
Sbjct: 289 KGHFGPINALAFNPDGKSFSSGGEDGY 315

[Close]

BLAST of Lag0005423.1 vs. NCBI nr
Match: CAG7895406.1 (unnamed protein product [Brassica rapa])

HSP 1 Score: 104.4 bits (259), Expect = 6.4e-19
Identity = 57/87 (65.52%), Postives = 61/87 (70.11%), Query Frame = 0


Query: 17  MLDCLVDDVKISFDSYRSLGFEVVLGGGQDASAV-ITDHRVGKFEAKFYHKILQVDIGGV 76
           +L   V  V ++  S   L   VVLGGGQDASAV  TDHR GKFEAKFY KILQ +IGGV
Sbjct: 512 LLKTYVSGVPVNAVSMSPLLDHVVLGGGQDASAVTTTDHRAGKFEAKFYDKILQEEIGGV 571

Query: 77  NDHFLPINALAFNPDGKSFSIRGELDY 103
             HF PINALAFNPDGKSFS  GE  Y
Sbjct: 572 KGHFGPINALAFNPDGKSFSSGGEDGY 598

[Close]

BLAST of Lag0005423.1 vs. ExPASy Swiss-Prot
Match: Q38884 (Eukaryotic translation initiation factor 3 subunit I OS=Arabidopsis thaliana OX=3702 GN=TIF3I1 PE=2 SV=2)

HSP 1 Score: 101.7 bits (252), Expect = 5.4e-21
Identity = 52/65 (80.00%), Postives = 53/65 (81.54%), Query Frame = 0


Query: 39  VVLGGGQDASAV-ITDHRVGKFEAKFYHKILQVDIGGVNDHFLPINALAFNPDGKSFSIR 98
           VVLGGGQDASAV  TDHR GKFEAKFY KILQ +IGGV  HF PINALAFNPDGKSFS  
Sbjct: 249 VVLGGGQDASAVTTTDHRAGKFEAKFYDKILQEEIGGVKGHFGPINALAFNPDGKSFSSG 308

Query: 99  GELDY 103
           GE  Y
Sbjct: 309 GEDGY 313

[Close]

BLAST of Lag0005423.1 vs. ExPASy Swiss-Prot
Match: Q759L2 (Eukaryotic translation initiation factor 3 subunit I OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=TIF34 PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 8.4e-14
Identity = 37/62 (59.68%), Postives = 46/62 (74.19%), Query Frame = 0


Query: 39  VVLGGGQDASAV-ITDHRVGKFEAKFYHKILQVDIGGVNDHFLPINALAFNPDGKSFSIR 98
           V+LGGGQDA  V  T  R GKFEAKFYHK+ QV+IG V+DHF P+N +A +P G S++  
Sbjct: 250 VILGGGQDARDVTTTSAREGKFEAKFYHKLFQVEIGRVDDHFGPVNYIAVSPQGTSYASG 309

Query: 99  GE 100
           GE
Sbjct: 310 GE 311

[Close]

BLAST of Lag0005423.1 vs. ExPASy Swiss-Prot
Match: A7RM20 (Eukaryotic translation initiation factor 3 subunit I OS=Nematostella vectensis OX=45351 GN=v1g239264 PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 3.2e-13
Identity = 39/77 (50.65%), Postives = 53/77 (68.83%), Query Frame = 0


Query: 39  VVLGGGQDASAV-ITDHRVGKFEAKFYHKILQVDIGGVNDHFLPINALAFNPDGKSFSIR 98
           VV+GGGQ+A  V  T  RVGKF+A+F+H + + +IG V  HF PIN+LAF+PDG+S+S  
Sbjct: 240 VVVGGGQEAMEVTTTSTRVGKFDARFFHVVFEEEIGRVKGHFGPINSLAFHPDGRSYSSG 299

Query: 99  GELDY------EGNYDD 109
           GE  Y      + +YDD
Sbjct: 300 GEDGYVRIHSFDPSYDD 316

[Close]

BLAST of Lag0005423.1 vs. ExPASy Swiss-Prot
Match: Q66J51 (Eukaryotic translation initiation factor 3 subunit I OS=Xenopus laevis OX=8355 GN=eif3i PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 4.2e-13
Identity = 37/65 (56.92%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 39  VVLGGGQDASAV-ITDHRVGKFEAKFYHKILQVDIGGVNDHFLPINALAFNPDGKSFSIR 98
           VVLGGGQ+A  V  T  R+GKFEA+F+H   + + G V  HF PIN+LAF+PDGKS+S  
Sbjct: 244 VVLGGGQEAMDVTTTSTRIGKFEARFFHVAFEEEFGRVKGHFGPINSLAFHPDGKSYSSG 303

Query: 99  GELDY 103
           GE  Y
Sbjct: 304 GEDGY 308

[Close]

BLAST of Lag0005423.1 vs. ExPASy Swiss-Prot
Match: Q5EBE8 (Eukaryotic translation initiation factor 3 subunit I OS=Xenopus tropicalis OX=8364 GN=eif3i PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 4.2e-13
Identity = 37/65 (56.92%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 39  VVLGGGQDASAV-ITDHRVGKFEAKFYHKILQVDIGGVNDHFLPINALAFNPDGKSFSIR 98
           VVLGGGQ+A  V  T  R+GKFEA+F+H   + + G V  HF PIN+LAF+PDGKS+S  
Sbjct: 244 VVLGGGQEAMDVTTTSTRIGKFEARFFHVAFEEEFGRVKGHFGPINSLAFHPDGKSYSSG 303

Query: 99  GELDY 103
           GE  Y
Sbjct: 304 GEDGY 308

The following BLAST results are available for this feature:

BLAST of Lag0005423.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KZV44849.1	3.7e-19	81.54	eukaryotic translation initiation factor 3 subunit I [Dorcoceras hygrometricum]	[more]
KZV57115.1	3.7e-19	81.54	eukaryotic translation initiation factor 3 subunit I [Dorcoceras hygrometricum]	[more]
CAF2143618.1	6.4e-19	65.52	unnamed protein product [Brassica napus]	[more]
KAF2597520.1	6.4e-19	65.52	hypothetical protein F2Q68_00011701 [Brassica cretica]	[more]
CAG7895406.1	6.4e-19	65.52	unnamed protein product [Brassica rapa]	[more]

BLAST of Lag0005423.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q38884	5.4e-21	80.00	Eukaryotic translation initiation factor 3 subunit I OS=Arabidopsis thaliana OX=...	[more]
Q759L2	8.4e-14	59.68	Eukaryotic translation initiation factor 3 subunit I OS=Ashbya gossypii (strain ...	[more]
A7RM20	3.2e-13	50.65	Eukaryotic translation initiation factor 3 subunit I OS=Nematostella vectensis O...	[more]
Q66J51	4.2e-13	56.92	Eukaryotic translation initiation factor 3 subunit I OS=Xenopus laevis OX=8355 G...	[more]
Q5EBE8	4.2e-13	56.92	Eukaryotic translation initiation factor 3 subunit I OS=Xenopus tropicalis OX=83...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015943	WD40/YVTN repeat-like-containing domain superfamily	GENE3D	2.130.10.10		coord: 3..102 e-value: 1.5E-12 score: 49.3
None	No IPR available	PANTHER	PTHR19877:SF9	EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I	coord: 34..102
None	No IPR available	PANTHER	PTHR19877	EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I	coord: 34..102

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0005423	Lag0005423	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0005423.1.exon3	Lag0005423.1.exon3	exon
Lag0005423.1.exon2	Lag0005423.1.exon2	exon
Lag0005423.1.exon1	Lag0005423.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0005423.1	cds.Lag0005423.1_3	CDS
cds.Lag0005423.1	cds.Lag0005423.1_2	CDS
cds.Lag0005423.1	cds.Lag0005423.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0005423.1	Lag0005423.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001732	formation of cytoplasmic translation initiation complex
cellular_component	GO:0016282	eukaryotic 43S preinitiation complex
cellular_component	GO:0033290	eukaryotic 48S preinitiation complex
cellular_component	GO:0071541	eukaryotic translation initiation factor 3 complex, eIF3m
molecular_function	GO:0005515	protein binding
molecular_function	GO:0003743	translation initiation factor activity