Lag0005044.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0005044.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	lachrymatory-factor synthase
Location	chr6: 9949749 .. 9953423 (-)
Sequence length	705
RNA-Seq Expression	Lag0005044.1
Synteny	Lag0005044.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGAAAACTCCGCAGCAAAGTGGGAAGGCCGAGTTTCAGAGGCGTTAACAGTCGCCGACGCCGACCAGATATGGCCGATGCTCAAGGACTTCTTCAACTTCCACAAATGGTTTCCAACCCTAGCCAATTGCTACGGACTTTCAGGCGCTAACGGCGAGGTCGGCTGCGTCCGATTCTGCTCCGGCTTCTCCATTCCATCCACCGCCGCCTCCGATCGCGTCGTTAGCTGGTCCAAAGAACGGCTGCTGGCTCTGGACGACGACCGCCGCCGCATATGCTACGAGATCGTTGATAGCAACATCGGATTCAAATCCTACGTGTCGACCATCGAGATCGCTCCGGGCGGCGGCGTGATTGAATGGAGCTTCGCCGTTGAGCCGGTGGAAGGGTTGAAGCTGGAGGACCTGGTGAAGAAATTCCAAGGGGGATTGAGTTCCATGGCCAAGCGAATGGAGGCTGCGGTAGAAAAAAATTAGGGCAATCACAATTAATAATTCACAGTGGCTTTGATTGTAGTAAATTTTTATTTTTACTTTTTGGAAGGATTCAAATTTCTAACATTTGCTCATGGTAATAATGATGTTGAAATTTGTCACCTACTATGAATTCATCTGTTGAACCGATATAAAGTACGTGTTTATGATCAAGAGATTTATCCGAGTTCGAATTTCCATGGTGATGAAATATGTGTCTAGAAAAAATAATTATAATATGAAGTAAATAAATATTTTTCAAAATTATAACTTTACCATGAAAATATAAATAACGGATGAGGGAGAGTTATAATATTATACCATCTTTAGATGATATTATTTAAATTAAATTATAAGTTTAGTTTATGAGGTTTCAATATCTGTGTCTAATATCTATTTGTTAAAACAAAACAAATAATAAGAAGAGAAAACGAGAAGAAACGACACAAATTTAATTACGTGATTCACCAATGATATGTTAGTTATATACCTGAGACGGAGAGAGAATAGTTCTATTATGTGAAGATATCAAATTAATTACAAATGGCATAAAAGAATAAGGATGTTTATATATGACACCCCTCTTAACTCTAACACCAAAAAATATAATAAAATCCAAAACGAGAAATAAACAAATAATCTCTATACAATTAAAGTTTAATGATAGATTCTGGGACACATATCAACAATATTCTTGACATATTTAAAATTATATACTTATATTTAAACTTGGTTGGTATTTCACAATAATTTTTTTTTATTAATCTAGAGATGTCAATTTACTCTCTTGATATGACATCGTTATAATTTTAGAAACTTTTGATGTTTGTTTTTGCTCATAATAGAGATTTTTTTGACAATATGTAGAGGTGGAATGATTCAAACCATAAACTTCTTGATCATTAATACATTTTTTTTTATCAATTAAACTATGCTCACGTTGGCGCTCATAACCAGGATTGTCGGCTTTCTATAAAAGATAGTTTGTCTCATCTTACTTTTTGTACCAAGAACCATGTTTTATGGCATGCTAGTTCCTTTTCCTTGTTTTATGAAGTATTTGGTTCATTTCGATAAATAAATAAATAATATTTGGTTAGGGAAAGAATAAGGAAATGGTTAGGAAGCTAGTAAAAAAAATTGAGTTGTTAGTTCTTACCAAGTTTAAGTTGTGTTTGGTTTAACTTTGTTTAATTTTGAAAAAAAATTATAGTGTTTGGCAACCGCTAAAAATGACTTATTAGAAAATGAGATTGATAATAAAATAAACCATTATGAAATAAACACTTGAAATCAATTTTTTAAAAAAGACTTTTTTAGTATACAATAACTTGGGTATACAAACACACTTGTGATGAAATGCAACCAAACATATGAATGTTTAAATTCTAAACAATTTTTATACAAAATGTTTTTTTTTTAATGCATTCTTGAAAAACATTTAAGTTAAAATGTATTCTATGGAGATCCTTAATGTTTTTAATTTGGATGTATCTAATTGAGAGCTTTTATCATTATCTCCTATTAAAGGTAATCATAATAATTTTTGTCATTCTCATTTAAGCCTTTATTTGGAATAATAACTTGGATGAGATCTATTATTCTTTCATTTCTTTTGAATAAATTCAAATTTATCAAATGAAAAAAATGAACAATAGTTTAAGTGTTGTGCTATTTCGTACATAAACACCACCGTTAAAATATTGACAACTAAGTGAGATAAAGCCTGAAAGTAGAAAATGAGATTTTTTTTCTTTTTATTAATTGTCAATATCTTAACGATGGTATGTAGAGAAAGATGTATAGTCAAATTTCATCCTTAAAAATATATATATTTACTAAACAAGTAAAAACGATAATTTTGGTTTCACAATGTAACTAAAAAAAACAAAAAAAAAAAAAAAAAAAGGAAAAAGTGGTTTCACACAATAGATATGAAAATGCACCCTCTTTTTAAGTTCTAAGAAATAATAATTAAAAAAAAAATTGTTTTTTGACAAAGAATTCAAAACCCTTTTCCAGTAATTAGTTTTATTTATTTATTTTTTATTTTCTTAATCCAATGGTGTAAAGATAGAAATTCAAACATTTTTTTCATAGTCAATCAATAGGTAAGATTTGAACTTGTGACTTTTTGTTTGAAGAGATAATGCATGCTTCAAACAGTTAAGGTCTCATTTGATGATTAATAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATTAAGCTTATAAACACCACTTACTTCTACACATTTCTTTGTTTTGTCATTTTACCACATTCTCATGGTTTTAAAAATCAAGTCAAAATTAGAAAGCTATAAACAATAGGGCCCGTTTGGTAACGTTCTCGTTTCTTGTTTCCGTTTCTTCATTTTTAATAAAGAAAAATAGAATTTCATGACCCTAACACCTATACATTCAATAATAATAAACCTATATTCTTATTATGTTTAGAAAAAATGGAAATAAGAACAAAAAAGAAGAAACTAGTTTTAGTTGTTCCTAAGGGCCCGTTTGGTACAAGATTTTTGGAGATGGGTGTTGAGTGAGAAATTTCGGCCCATCACAAAGTGCCACGTCATTAATTTGAATTAATGATAAAATAAATTTGGGACCAATTACAAATTTACACGTGTCCCTAAATTAAATTAATAGACATTAAAATTTGGCAAATCAAACAATGCCACTTGTCTATTATTGATCCATCAGATGACATAAGCCGAGTCCAATCAAATTATGCCATGTGGCAATTGGACTCGAGACTAATGTGACCCGAGATCATCGAAGAAATTGGGCTAAATTAAAATAATCCAATTTTATTAAAAATGAATTGGATTAAAGCCCAAAAGAGCCAAAGCCCAATGGTTGGGCCCAAGCCCAAGCCCATATGAGGTCCATCAAACTCTATAAATAGAGGAGGCCACCATTCATTTTGGGCATTCAGACAGAGTCAGAGAAGCCAGAGCCCAGAGCATTCTCCCAAGGTCCAGAGTCTTCAGAAGTCAGAGAGTTCAAAGAATTCAGAAGATCCAAGATTCAGAATTCGGCCATCTCCAGACTCAGAAGATCCAACGACTGAAAGACTCCAAAGAATCAACCGTTTCTTCATCAAGATACAAGTTGAAGATTCAACCTTCCTCCGTCTGAGATCAAGCTTGTCGGCCCTCAGATCAGACTCCATATTTTGA

mRNA sequence

ATGGCGGAAAACTCCGCAGCAAAGTGGGAAGGCCGAGTTTCAGAGGCGTTAACAGTCGCCGACGCCGACCAGATATGGCCGATGCTCAAGGACTTCTTCAACTTCCACAAATGGTTTCCAACCCTAGCCAATTGCTACGGACTTTCAGGCGCTAACGGCGAGGTCGGCTGCGTCCGATTCTGCTCCGGCTTCTCCATTCCATCCACCGCCGCCTCCGATCGCGTCGTTAGCTGGTCCAAAGAACGGCTGCTGGCTCTGGACGACGACCGCCGCCGCATATGCTACGAGATCGTTGATAGCAACATCGGATTCAAATCCTACGTGTCGACCATCGAGATCGCTCCGGGCGGCGGCGTGATTGAATGGAGCTTCGCCGTTGAGCCGGTGGAAGGGTTGAAGCTGGAGGACCTGGTGAAGAAATTCCAAGGGGGATTGAGTTCCATGGCCAAGCGAATGGAGGCTGCGAGTCAGAGAAGCCAGAGCCCAGAGCATTCTCCCAAGGTCCAGAGTCTTCAGAAGTCAGAGAGTTCAAAGAATTCAGAAGATCCAAGATTCAGAATTCGGCCATCTCCAGACTCAGAAGATCCAACGACTGAAAGACTCCAAAGAATCAACCGTTTCTTCATCAAGATACAAGTTGAAGATTCAACCTTCCTCCGTCTGAGATCAAGCTTGTCGGCCCTCAGATCAGACTCCATATTTTGA

Coding sequence (CDS)

ATGGCGGAAAACTCCGCAGCAAAGTGGGAAGGCCGAGTTTCAGAGGCGTTAACAGTCGCCGACGCCGACCAGATATGGCCGATGCTCAAGGACTTCTTCAACTTCCACAAATGGTTTCCAACCCTAGCCAATTGCTACGGACTTTCAGGCGCTAACGGCGAGGTCGGCTGCGTCCGATTCTGCTCCGGCTTCTCCATTCCATCCACCGCCGCCTCCGATCGCGTCGTTAGCTGGTCCAAAGAACGGCTGCTGGCTCTGGACGACGACCGCCGCCGCATATGCTACGAGATCGTTGATAGCAACATCGGATTCAAATCCTACGTGTCGACCATCGAGATCGCTCCGGGCGGCGGCGTGATTGAATGGAGCTTCGCCGTTGAGCCGGTGGAAGGGTTGAAGCTGGAGGACCTGGTGAAGAAATTCCAAGGGGGATTGAGTTCCATGGCCAAGCGAATGGAGGCTGCGAGTCAGAGAAGCCAGAGCCCAGAGCATTCTCCCAAGGTCCAGAGTCTTCAGAAGTCAGAGAGTTCAAAGAATTCAGAAGATCCAAGATTCAGAATTCGGCCATCTCCAGACTCAGAAGATCCAACGACTGAAAGACTCCAAAGAATCAACCGTTTCTTCATCAAGATACAAGTTGAAGATTCAACCTTCCTCCGTCTGAGATCAAGCTTGTCGGCCCTCAGATCAGACTCCATATTTTGA

Protein sequence

MAENSAAKWEGRVSEALTVADADQIWPMLKDFFNFHKWFPTLANCYGLSGANGEVGCVRFCSGFSIPSTAASDRVVSWSKERLLALDDDRRRICYEIVDSNIGFKSYVSTIEIAPGGGVIEWSFAVEPVEGLKLEDLVKKFQGGLSSMAKRMEAASQRSQSPEHSPKVQSLQKSESSKNSEDPRFRIRPSPDSEDPTTERLQRINRFFIKIQVEDSTFLRLRSSLSALRSDSIF

Homology

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BLAST of Lag0005044.1 vs. NCBI nr
Match: XP_022131917.1 (lachrymatory-factor synthase [Momordica charantia])

HSP 1 Score: 276.2 bits (705), Expect = 2.7e-70
Identity = 135/157 (85.99%), Postives = 141/157 (89.81%), Query Frame = 0


Query: 1   MAENSAAKWEGRVSEALTVADADQIWPMLKDFFNFHKWFPTLANCYGLSGANGEVGCVRF 60
           MAE S AKWEGRVSEALTVA ADQIWPMLKDFFNFHKWFPTLA CYG++G NGEVGCVRF
Sbjct: 1   MAETSPAKWEGRVSEALTVASADQIWPMLKDFFNFHKWFPTLATCYGIAGTNGEVGCVRF 60

Query: 61  CSGFSIPSTAASDRVVSWSKERLLALDDDRRRICYEIVDSNIGFKSYVSTIEIAPGG--G 120
           CSGFSIPS   +DRVVSWSKERLLA+DDD RRI YEIVDSNIGFKSYV+TIEIAPGG  G
Sbjct: 61  CSGFSIPSLDGADRVVSWSKERLLAVDDDSRRIRYEIVDSNIGFKSYVATIEIAPGGGDG 120

Query: 121 VIEWSFAVEPVEGLKLEDLVKKFQGGLSSMAKRMEAA 156
           VIEW FAVEPVEGLKLEDLVKK+Q GL SM KRMEAA
Sbjct: 121 VIEWRFAVEPVEGLKLEDLVKKYQEGLRSMVKRMEAA 157

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BLAST of Lag0005044.1 vs. NCBI nr
Match: XP_023537781.1 (lachrymatory-factor synthase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 271.9 bits (694), Expect = 5.0e-69
Identity = 134/159 (84.28%), Postives = 141/159 (88.68%), Query Frame = 0


Query: 1   MAENSAAKWEGRVSEALTVADADQIWPMLKDFFNFHKWFPTLANCYGLSGANGEVGCVRF 60
           MAEN+AAKWEGRVSEAL  A ADQIWPMLKDFFNFHKWFPTLANCYGLSG NGEVGCVRF
Sbjct: 1   MAENTAAKWEGRVSEALQGATADQIWPMLKDFFNFHKWFPTLANCYGLSGTNGEVGCVRF 60

Query: 61  CSGFSIPSTAASDRVVSWSKERLLALDDDRRRICYEIVDSNIGFKSYVSTIEIAPG--GG 120
           CSGFSIPST  SD VVSWSKERL+A+DD+RRRI YEIVDSNIGFKSYVSTIE+  G  GG
Sbjct: 61  CSGFSIPSTDGSDSVVSWSKERLVAVDDERRRIRYEIVDSNIGFKSYVSTIEVVEGGDGG 120

Query: 121 VIEWSFAVEPVEGLKLEDLVKKFQGGLSSMAKRMEAASQ 158
           VIEWSF VE VEGLKLEDLVKKF+ GL SMA+RME A Q
Sbjct: 121 VIEWSFVVEAVEGLKLEDLVKKFETGLKSMARRMEVAVQ 159

[Close]

BLAST of Lag0005044.1 vs. NCBI nr
Match: XP_038886400.1 (lachrymatory-factor synthase [Benincasa hispida])

HSP 1 Score: 271.6 bits (693), Expect = 6.5e-69
Identity = 131/161 (81.37%), Postives = 143/161 (88.82%), Query Frame = 0


Query: 1   MAENSAAKWEGRVSEALTVADADQIWPMLKDFFNFHKWFPTLANCYGLSGANGEVGCVRF 60
           MA+NSAAKWEGRV+EALTVA ADQ WPM+KDFFNFHKWFPTLANCYGLSG NGEVGCVRF
Sbjct: 24  MAQNSAAKWEGRVAEALTVAAADQTWPMIKDFFNFHKWFPTLANCYGLSGINGEVGCVRF 83

Query: 61  CSGFSIPSTAASDRVVSWSKERLLALDDDRRRICYEIVDSNIGFKSYVSTIEIAPGGG-- 120
           CSGFSIPST  SDRVVSWSKERL+ +DD+RRRICYEIVDSNIGFKSY +TIE+A GGG  
Sbjct: 84  CSGFSIPSTDGSDRVVSWSKERLVEIDDERRRICYEIVDSNIGFKSYAATIEVAEGGGSS 143

Query: 121 --VIEWSFAVEPVEGLKLEDLVKKFQGGLSSMAKRMEAASQ 158
             VIEW F VE VEGL ++DLVKK+Q GLSSMAKRM+AA Q
Sbjct: 144 SCVIEWRFEVEAVEGLTVDDLVKKYQVGLSSMAKRMDAAVQ 184

[Close]

BLAST of Lag0005044.1 vs. NCBI nr
Match: KAG6585381.1 (Lachrymatory-factor synthase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020297.1 Lachrymatory-factor synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 271.2 bits (692), Expect = 8.5e-69
Identity = 134/161 (83.23%), Postives = 142/161 (88.20%), Query Frame = 0


Query: 1   MAENSAAKWEGRVSEALTVADADQIWPMLKDFFNFHKWFPTLANCYGLSGANGEVGCVRF 60
           MAENSAAKWEGRVSEAL  A ADQIWPMLKDFFN HKWFPTLANCYGLSG NGEVGCVRF
Sbjct: 1   MAENSAAKWEGRVSEALQGATADQIWPMLKDFFNLHKWFPTLANCYGLSGTNGEVGCVRF 60

Query: 61  CSGFSIPSTAASDRVVSWSKERLLALDDDRRRICYEIVDSNIGFKSYVSTIEIAPG--GG 120
           CSGFSIPST  SD VVSWSKERL+A+DD+RRRI YEIVDSNIGFKSYVSTIE+  G  GG
Sbjct: 61  CSGFSIPSTDGSDSVVSWSKERLVAVDDERRRIRYEIVDSNIGFKSYVSTIEVVEGGDGG 120

Query: 121 VIEWSFAVEPVEGLKLEDLVKKFQGGLSSMAKRMEAASQRS 160
           VIEWSF VE VEGLKLEDLVKKF+ GL SMA+RME A Q++
Sbjct: 121 VIEWSFVVEAVEGLKLEDLVKKFETGLKSMARRMEVAVQKN 161

[Close]

BLAST of Lag0005044.1 vs. NCBI nr
Match: XP_023002531.1 (lachrymatory-factor synthase-like [Cucurbita maxima])

HSP 1 Score: 269.6 bits (688), Expect = 2.5e-68
Identity = 133/161 (82.61%), Postives = 141/161 (87.58%), Query Frame = 0


Query: 1   MAENSAAKWEGRVSEALTVADADQIWPMLKDFFNFHKWFPTLANCYGLSGANGEVGCVRF 60
           MAENSAAKWEGRVSE L  A ADQIWPMLKDFFNFHKWFPTLANCYGLSG NGEVGCVRF
Sbjct: 1   MAENSAAKWEGRVSETLQGATADQIWPMLKDFFNFHKWFPTLANCYGLSGTNGEVGCVRF 60

Query: 61  CSGFSIPSTAASDRVVSWSKERLLALDDDRRRICYEIVDSNIGFKSYVSTIEIAPG--GG 120
           CSGFSIPST  SD VVSWSKERL+ +DD+RRRI YEIVDSNIGFKSYVSTI++  G  GG
Sbjct: 61  CSGFSIPSTDGSDSVVSWSKERLVTVDDERRRIRYEIVDSNIGFKSYVSTIKVVEGGDGG 120

Query: 121 VIEWSFAVEPVEGLKLEDLVKKFQGGLSSMAKRMEAASQRS 160
           VIEWSF VE VEGLKLEDLVKKF+ GL SMAKRME A Q++
Sbjct: 121 VIEWSFVVEAVEGLKLEDLVKKFERGLKSMAKRMEVAVQQN 161

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BLAST of Lag0005044.1 vs. ExPASy Swiss-Prot
Match: P59082 (Lachrymatory-factor synthase OS=Allium cepa OX=4679 GN=LFS PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.7e-17
Identity = 48/154 (31.17%), Postives = 75/154 (48.70%), Query Frame = 0


Query: 4   NSAAKWEGRVSEALTVADADQIWPMLKDFFNFHKWFPTLANCYGLSGANGEVGCVRFCSG 63
           N A KW G+V   L     +Q W +LKDF N HK  P+L+ C  + G    VGCVR+  G
Sbjct: 16  NGARKWSGKVHALLPNTKPEQAWTLLKDFINLHKVMPSLSVCELVEGEANVVGCVRYVKG 75

Query: 64  FSIPSTAASDRVVSWSKERLLALDDDRRRICYEIVDSNIGFKSYVSTIEIAPG----GGV 123
              P          W+KE+L+ALD+      Y   +   G++ Y +T++I  G    G  
Sbjct: 76  IMHPIEEE-----FWAKEKLVALDNKNMSYSYIFTECFTGYEDYTATMQIVEGPEHKGSR 135

Query: 124 IEWSFAVEPVEGLKLEDLVKKFQGGLSSMAKRME 154
            +WSF  + +EG+      +  Q   + + +++E
Sbjct: 136 FDWSFQCKYIEGMTESAFTEILQHWATEIGQKIE 164

[Close]

BLAST of Lag0005044.1 vs. ExPASy TrEMBL
Match: A0A6J1BSC0 (lachrymatory-factor synthase OS=Momordica charantia OX=3673 GN=LOC111004938 PE=3 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 1.3e-70
Identity = 135/157 (85.99%), Postives = 141/157 (89.81%), Query Frame = 0


Query: 1   MAENSAAKWEGRVSEALTVADADQIWPMLKDFFNFHKWFPTLANCYGLSGANGEVGCVRF 60
           MAE S AKWEGRVSEALTVA ADQIWPMLKDFFNFHKWFPTLA CYG++G NGEVGCVRF
Sbjct: 1   MAETSPAKWEGRVSEALTVASADQIWPMLKDFFNFHKWFPTLATCYGIAGTNGEVGCVRF 60

Query: 61  CSGFSIPSTAASDRVVSWSKERLLALDDDRRRICYEIVDSNIGFKSYVSTIEIAPGG--G 120
           CSGFSIPS   +DRVVSWSKERLLA+DDD RRI YEIVDSNIGFKSYV+TIEIAPGG  G
Sbjct: 61  CSGFSIPSLDGADRVVSWSKERLLAVDDDSRRIRYEIVDSNIGFKSYVATIEIAPGGGDG 120

Query: 121 VIEWSFAVEPVEGLKLEDLVKKFQGGLSSMAKRMEAA 156
           VIEW FAVEPVEGLKLEDLVKK+Q GL SM KRMEAA
Sbjct: 121 VIEWRFAVEPVEGLKLEDLVKKYQEGLRSMVKRMEAA 157

[Close]

BLAST of Lag0005044.1 vs. ExPASy TrEMBL
Match: A0A6J1KLK1 (lachrymatory-factor synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111496344 PE=3 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 1.2e-68
Identity = 133/161 (82.61%), Postives = 141/161 (87.58%), Query Frame = 0


Query: 1   MAENSAAKWEGRVSEALTVADADQIWPMLKDFFNFHKWFPTLANCYGLSGANGEVGCVRF 60
           MAENSAAKWEGRVSE L  A ADQIWPMLKDFFNFHKWFPTLANCYGLSG NGEVGCVRF
Sbjct: 1   MAENSAAKWEGRVSETLQGATADQIWPMLKDFFNFHKWFPTLANCYGLSGTNGEVGCVRF 60

Query: 61  CSGFSIPSTAASDRVVSWSKERLLALDDDRRRICYEIVDSNIGFKSYVSTIEIAPG--GG 120
           CSGFSIPST  SD VVSWSKERL+ +DD+RRRI YEIVDSNIGFKSYVSTI++  G  GG
Sbjct: 61  CSGFSIPSTDGSDSVVSWSKERLVTVDDERRRIRYEIVDSNIGFKSYVSTIKVVEGGDGG 120

Query: 121 VIEWSFAVEPVEGLKLEDLVKKFQGGLSSMAKRMEAASQRS 160
           VIEWSF VE VEGLKLEDLVKKF+ GL SMAKRME A Q++
Sbjct: 121 VIEWSFVVEAVEGLKLEDLVKKFERGLKSMAKRMEVAVQQN 161

[Close]

BLAST of Lag0005044.1 vs. ExPASy TrEMBL
Match: A0A6J1GHW7 (lachrymatory-factor synthase OS=Cucurbita moschata OX=3662 GN=LOC111454336 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 2.7e-68
Identity = 133/161 (82.61%), Postives = 141/161 (87.58%), Query Frame = 0


Query: 1   MAENSAAKWEGRVSEALTVADADQIWPMLKDFFNFHKWFPTLANCYGLSGANGEVGCVRF 60
           MAENSAAKWEGRVSEAL  A ADQIWPMLKDFFN HKWFPTLANCYGLSG NGEVGCVRF
Sbjct: 1   MAENSAAKWEGRVSEALQGATADQIWPMLKDFFNLHKWFPTLANCYGLSGTNGEVGCVRF 60

Query: 61  CSGFSIPSTAASDRVVSWSKERLLALDDDRRRICYEIVDSNIGFKSYVSTIEIAPG--GG 120
           CSGFSIPST  S  VVSWSKERL+A+DD+RRRI YEIVDSNIGFKSYVSTIE+  G  GG
Sbjct: 61  CSGFSIPSTDGSGSVVSWSKERLVAVDDERRRIRYEIVDSNIGFKSYVSTIEVVEGGDGG 120

Query: 121 VIEWSFAVEPVEGLKLEDLVKKFQGGLSSMAKRMEAASQRS 160
           VIEWSF VE VEGLKLEDLVKKF+ GL SMA+RME A Q++
Sbjct: 121 VIEWSFVVEAVEGLKLEDLVKKFETGLKSMARRMEVAVQKN 161

[Close]

BLAST of Lag0005044.1 vs. ExPASy TrEMBL
Match: A0A0A0LPR8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G373500 PE=3 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 7.1e-61
Identity = 117/153 (76.47%), Postives = 131/153 (85.62%), Query Frame = 0


Query: 6   AAKWEGRVSEALTVADADQIWPMLKDFFNFHKWFPTLANCYGLSGANGEVGCVRFCSGFS 65
           AAKWEGRV+E LTVA  DQIWPM+KDFFNFHKWFPTLANCYGLSG N EVG VRFCSGFS
Sbjct: 18  AAKWEGRVTETLTVATPDQIWPMIKDFFNFHKWFPTLANCYGLSGTNAEVGSVRFCSGFS 77

Query: 66  IPSTAASDRVVSWSKERLLALDDDRRRICYEIVDSNIGFKSYVSTIE---IAPGGGVIEW 125
           IPS+  SD VVSWSKERL+ +D++ RRICYEIVDSNIGFKSYV+T+E   ++ GG +IEW
Sbjct: 78  IPSSDGSDGVVSWSKERLVGVDEEHRRICYEIVDSNIGFKSYVATMEVGSVSGGGCMIEW 137

Query: 126 SFAVEPVEGLKLEDLVKKFQGGLSSMAKRMEAA 156
            F VE VEGLKLEDLVKK++ GL SMA RMEAA
Sbjct: 138 RFEVEAVEGLKLEDLVKKYEVGLRSMANRMEAA 170

[Close]

BLAST of Lag0005044.1 vs. ExPASy TrEMBL
Match: A0A5A7VFE2 (Lachrymatory-factor synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003740 PE=3 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 1.2e-60
Identity = 119/160 (74.38%), Postives = 136/160 (85.00%), Query Frame = 0


Query: 1   MAENSAAKWEGRVSEALTVADADQIWPMLKDFFNFHKWFPTLANCYGLSGA-NGEVGCVR 60
           MAEN A+KWEGRV++ LT+A  DQIWPM+KDFFNFHKWFPTLANCYGLSG  N EVG VR
Sbjct: 17  MAENPASKWEGRVTQTLTLATPDQIWPMIKDFFNFHKWFPTLANCYGLSGTNNAEVGSVR 76

Query: 61  FCSGFSIPSTA-ASDRVVSWSKERLLALDDDRRRICYEIVDSNIGFKSYVSTIEIAPGGG 120
           FCSGFSIPS++  SD VVSWSKERL+ +D++ RRICYE+VDSNIGFKSYV+T+E+  GGG
Sbjct: 77  FCSGFSIPSSSDGSDGVVSWSKERLVGVDEEHRRICYEMVDSNIGFKSYVATMEVGEGGG 136

Query: 121 ---VIEWSFAVEPVEGLKLEDLVKKFQGGLSSMAKRMEAA 156
              VIEW F VE VEGLKLEDLVKK++ GL SMA RMEAA
Sbjct: 137 GGCVIEWRFEVEAVEGLKLEDLVKKYEVGLRSMANRMEAA 176

[Close]

BLAST of Lag0005044.1 vs. TAIR 10
Match: AT2G25770.1 (Polyketide cyclase/dehydrase and lipid transport superfamily protein )

HSP 1 Score: 174.5 bits (441), Expect = 1.0e-43
Identity = 86/158 (54.43%), Postives = 108/158 (68.35%), Query Frame = 0


Query: 4   NSAAKWEGRVSEALTVADADQIWPMLKDFFNFHKWFPTLANCYGLSGANGEVGCVRFCSG 63
           +S  KW  +VS  LT A  D+IWP+  DFFN HKW PTLA C+G+ G NGE GC+RFCSG
Sbjct: 5   SSPEKWLAKVSVTLTKAKPDEIWPLFTDFFNLHKWLPTLATCHGVHGNNGEQGCIRFCSG 64

Query: 64  FSIPSTAASDRVVSWSKERLLALDDDRRRICYEIVDSNIGFKSYVSTIEIAP---GGGVI 123
           FSI S    D    WSKE+L+A++   R + YEIV+SN GF+SYVST++I P    G VI
Sbjct: 65  FSIGSNGV-DSAARWSKEKLVAVNPVERVMRYEIVESNTGFESYVSTVKILPRGEDGCVI 124

Query: 124 EWSFAVEPVEGLKLEDLVKKFQGGLSSMAKRMEAASQR 159
           EWSF V+PV GL LE+LVKK++  L  + K ME  + R
Sbjct: 125 EWSFTVDPVRGLSLENLVKKYEKALEIITKNMEEDALR 161

[Close]

BLAST of Lag0005044.1 vs. TAIR 10
Match: AT2G25770.2 (Polyketide cyclase/dehydrase and lipid transport superfamily protein )

HSP 1 Score: 174.5 bits (441), Expect = 1.0e-43
Identity = 86/158 (54.43%), Postives = 108/158 (68.35%), Query Frame = 0


Query: 4   NSAAKWEGRVSEALTVADADQIWPMLKDFFNFHKWFPTLANCYGLSGANGEVGCVRFCSG 63
           +S  KW  +VS  LT A  D+IWP+  DFFN HKW PTLA C+G+ G NGE GC+RFCSG
Sbjct: 5   SSPEKWLAKVSVTLTKAKPDEIWPLFTDFFNLHKWLPTLATCHGVHGNNGEQGCIRFCSG 64

Query: 64  FSIPSTAASDRVVSWSKERLLALDDDRRRICYEIVDSNIGFKSYVSTIEIAP---GGGVI 123
           FSI S    D    WSKE+L+A++   R + YEIV+SN GF+SYVST++I P    G VI
Sbjct: 65  FSIGSNGV-DSAARWSKEKLVAVNPVERVMRYEIVESNTGFESYVSTVKILPRGEDGCVI 124

Query: 124 EWSFAVEPVEGLKLEDLVKKFQGGLSSMAKRMEAASQR 159
           EWSF V+PV GL LE+LVKK++  L  + K ME  + R
Sbjct: 125 EWSFTVDPVRGLSLENLVKKYEKALEIITKNMEEDALR 161

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BLAST of Lag0005044.1 vs. TAIR 10
Match: AT4G32870.1 (Polyketide cyclase/dehydrase and lipid transport superfamily protein )

HSP 1 Score: 120.6 bits (301), Expect = 1.7e-27
Identity = 57/156 (36.54%), Postives = 90/156 (57.69%), Query Frame = 0


Query: 3   ENSAAKWEGRVSEALTVADADQIWPMLKDFFNFHKWFPTLANCYGLSGANGEVGCVRFCS 62
           E  + KWEG+    +    A+++W +  DF N  +WFP++  CY + G +G  G +R+CS
Sbjct: 4   ETVSFKWEGKKVAQVNGVTAEKVWSVFSDFCNVQEWFPSVDTCYRVQGTDGVPGLIRYCS 63

Query: 63  GFSIPSTAASDRVVSWSKERLLALDDDRRRICYEIVDSNIGFKSYVSTIEIAPGGGV--- 122
                +T   +    W+KE+L+ +D   R + YEI+++N+GF+SYV+T+++ P  G    
Sbjct: 64  -----TTKTKEEGSRWAKEKLVKIDPIGRCLSYEILENNVGFRSYVATVQVTPVDGEDQV 123

Query: 123 --IEWSFAVEPVEGLKLEDLVKKFQGGLSSMAKRME 154
             IEWSF  +PV+G K EDL       L  MA +ME
Sbjct: 124 SRIEWSFVADPVDGWKKEDLESYVDFCLQHMANKME 154

The following BLAST results are available for this feature:

BLAST of Lag0005044.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131917.1	2.7e-70	85.99	lachrymatory-factor synthase [Momordica charantia]	[more]
XP_023537781.1	5.0e-69	84.28	lachrymatory-factor synthase [Cucurbita pepo subsp. pepo]	[more]
XP_038886400.1	6.5e-69	81.37	lachrymatory-factor synthase [Benincasa hispida]	[more]
KAG6585381.1	8.5e-69	83.23	Lachrymatory-factor synthase, partial [Cucurbita argyrosperma subsp. sororia] >K...	[more]
XP_023002531.1	2.5e-68	82.61	lachrymatory-factor synthase-like [Cucurbita maxima]	[more]

BLAST of Lag0005044.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P59082	2.7e-17	31.17	Lachrymatory-factor synthase OS=Allium cepa OX=4679 GN=LFS PE=1 SV=1	[more]

BLAST of Lag0005044.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BSC0	1.3e-70	85.99	lachrymatory-factor synthase OS=Momordica charantia OX=3673 GN=LOC111004938 PE=3...	[more]
A0A6J1KLK1	1.2e-68	82.61	lachrymatory-factor synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111496344 PE...	[more]
A0A6J1GHW7	2.7e-68	82.61	lachrymatory-factor synthase OS=Cucurbita moschata OX=3662 GN=LOC111454336 PE=3 ...	[more]
A0A0A0LPR8	7.1e-61	76.47	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G373500 PE=3 SV=1	[more]
A0A5A7VFE2	1.2e-60	74.38	Lachrymatory-factor synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca...	[more]

BLAST of Lag0005044.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G25770.1	1.0e-43	54.43	Polyketide cyclase/dehydrase and lipid transport superfamily protein	[more]
AT2G25770.2	1.0e-43	54.43	Polyketide cyclase/dehydrase and lipid transport superfamily protein	[more]
AT4G32870.1	1.7e-27	36.54	Polyketide cyclase/dehydrase and lipid transport superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023393	START-like domain superfamily	GENE3D	3.30.530.20		coord: 2..161 e-value: 3.9E-47 score: 162.6
IPR019587	Polyketide cyclase/dehydrase	PFAM	PF10604	Polyketide_cyc2	coord: 20..153 e-value: 5.6E-21 score: 75.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 150..199
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 152..176
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 177..199
None	No IPR available	PANTHER	PTHR31213:SF18	POLYKETIDE CYCLASE/DEHYDRASE AND LIPID TRANSPORTER	coord: 5..157
None	No IPR available	PANTHER	PTHR31213	FAMILY NOT NAMED	coord: 5..157
None	No IPR available	CDD	cd07821	PYR_PYL_RCAR_like	coord: 12..153 e-value: 2.09774E-34 score: 118.195
None	No IPR available	SUPERFAMILY	55961	Bet v1-like	coord: 18..155

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0005044	Lag0005044	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0005044.1.exon2	Lag0005044.1.exon2	exon
Lag0005044.1.exon1	Lag0005044.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0005044.1	cds.Lag0005044.1_2	CDS
cds.Lag0005044.1	cds.Lag0005044.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0005044.1	Lag0005044.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009738	abscisic acid-activated signaling pathway
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0080163	regulation of protein serine/threonine phosphatase activity
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0010427	abscisic acid binding
molecular_function	GO:0004864	protein phosphatase inhibitor activity
molecular_function	GO:0038023	signaling receptor activity