Lag0004664.1 (mRNA) Sponge gourd (AG‐4) v1

Overview


Name	Lag0004664.1
Type	mRNA
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Alpha-galactosidase
Location	chr6: 5883139 .. 5884292 (+)
Sequence length	708
RNA-Seq Expression	Lag0004664.1
Synteny	Lag0004664.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGACGTCTTTTTTCGCTCTCTCTGCTTTTGCCTCTTCTTCTGTTTTGGATTTCTCTTTAATTGGTCTGTTCATCTCTCCTTTCTCTCGCTTTTACTTCTAGTAGCTTGGATTGTTGTTTAAAGAATTCGATTTCCATATTTAGAATAGAATTGTCTGTGAAAACTGGGGTTGTTATTGATTGAACGAAGGCCACACTTTGTATGTTTTAATCTTAATGGATTCAATTTCTTCCCCTTTGTTCCTACTGGCTTGACTAATCATATGGTGCATACGAACTCTAATCTGTTGCAGGGCGTCCTCTCAAATTGGAGCAGAACGGGCAGCCCTCCCGCCGAGAGGCTGGAACTCCTATGACTCATTTTGCTGGACTGTTTCTGAAGAGGAGTTCCTAAACAATGCTGAGATTGTTGCCAAGCGGCTTAGTTCCAAGGGTTATGAGGTAGGAATTTGTTAACTGGCTGACGAGTTTGTTTGTAGCATTGGTCGTTTATTATCTAATGTAATGAACTGGAATGCTGGTAAGAAGATTGATGGTCTCTGGTTTATTTGATTGATGCAGTATGTGGTTGTGGATTACCTCTGGTATAGGAAGAAGGTTCCTGGTGCTTACACCGATTCTCTTGGATTTGATGTGATTGATGAATGGGGGAGGATGGTCCCGGACCCGGTTCGGTGGCCTTCCTCTCAAGGTGGGAAGGGGTTCTCTGAAGTAGCAAAGAAAGTTCATGATATGGGGTTGAAGTTTGGAATTCATATCATGAGAGGAATAAGTACACAAGCAGTAAATGCCAATACTCCTATATTGGACATTTCAAAGGTACCTTGTGCAAGTTCCCTTACATAAGTGAGCATTGATAAATTGTCAGCCCTAAAACATAGAAGAATTGTTAATTTTGTCCTTAATTGCTCCTTAGGGAGGTGCCTATGTAGAATCCGGGAGAAAATGGTTTGCAAGTGATATAGGGATAAAGTCGAGGGCTTGTGGATGGATGCGTAACGGCTTCATGAGTGTAAACGTTAATTCAGGAGCTGGAAAGGCCTTCTTAAGGTCCCTTTTTCAACAATATGCTGATTGGGGTGTTGATTTTGGTAAGTTGACTGTCATTAGAAAGCAAGGTCACTTCTTACATGCCTACATACCTTTGTAG

mRNA sequence

ATGAAAGACGTCTTTTTTCGCTCTCTCTGCTTTTGCCTCTTCTTCTGTTTTGGATTTCTCTTTAATTGGGCGTCCTCTCAAATTGGAGCAGAACGGGCAGCCCTCCCGCCGAGAGGCTGGAACTCCTATGACTCATTTTGCTGGACTGTTTCTGAAGAGGAGTTCCTAAACAATGCTGAGATTGTTGCCAAGCGGCTTAGTTCCAAGGGTTATGAGTATGTGGTTGTGGATTACCTCTGGTATAGGAAGAAGGTTCCTGGTGCTTACACCGATTCTCTTGGATTTGATGTGATTGATGAATGGGGGAGGATGGTCCCGGACCCGGTTCGGTGGCCTTCCTCTCAAGGTGGGAAGGGGTTCTCTGAAGTAGCAAAGAAAGTTCATGATATGGGGTTGAAGTTTGGAATTCATATCATGAGAGGAATAAGTACACAAGCAGTAAATGCCAATACTCCTATATTGGACATTTCAAAGGGAGGTGCCTATGTAGAATCCGGGAGAAAATGGTTTGCAAGTGATATAGGGATAAAGTCGAGGGCTTGTGGATGGATGCGTAACGGCTTCATGAGTGTAAACGTTAATTCAGGAGCTGGAAAGGCCTTCTTAAGGTCCCTTTTTCAACAATATGCTGATTGGGGTGTTGATTTTGGTAAGTTGACTGTCATTAGAAAGCAAGGTCACTTCTTACATGCCTACATACCTTTGTAG

Coding sequence (CDS)

ATGAAAGACGTCTTTTTTCGCTCTCTCTGCTTTTGCCTCTTCTTCTGTTTTGGATTTCTCTTTAATTGGGCGTCCTCTCAAATTGGAGCAGAACGGGCAGCCCTCCCGCCGAGAGGCTGGAACTCCTATGACTCATTTTGCTGGACTGTTTCTGAAGAGGAGTTCCTAAACAATGCTGAGATTGTTGCCAAGCGGCTTAGTTCCAAGGGTTATGAGTATGTGGTTGTGGATTACCTCTGGTATAGGAAGAAGGTTCCTGGTGCTTACACCGATTCTCTTGGATTTGATGTGATTGATGAATGGGGGAGGATGGTCCCGGACCCGGTTCGGTGGCCTTCCTCTCAAGGTGGGAAGGGGTTCTCTGAAGTAGCAAAGAAAGTTCATGATATGGGGTTGAAGTTTGGAATTCATATCATGAGAGGAATAAGTACACAAGCAGTAAATGCCAATACTCCTATATTGGACATTTCAAAGGGAGGTGCCTATGTAGAATCCGGGAGAAAATGGTTTGCAAGTGATATAGGGATAAAGTCGAGGGCTTGTGGATGGATGCGTAACGGCTTCATGAGTGTAAACGTTAATTCAGGAGCTGGAAAGGCCTTCTTAAGGTCCCTTTTTCAACAATATGCTGATTGGGGTGTTGATTTTGGTAAGTTGACTGTCATTAGAAAGCAAGGTCACTTCTTACATGCCTACATACCTTTGTAG

Protein sequence

MKDVFFRSLCFCLFFCFGFLFNWASSQIGAERAALPPRGWNSYDSFCWTVSEEEFLNNAEIVAKRLSSKGYEYVVVDYLWYRKKVPGAYTDSLGFDVIDEWGRMVPDPVRWPSSQGGKGFSEVAKKVHDMGLKFGIHIMRGISTQAVNANTPILDISKGGAYVESGRKWFASDIGIKSRACGWMRNGFMSVNVNSGAGKAFLRSLFQQYADWGVDFGKLTVIRKQGHFLHAYIPL

Homology

[Close]

BLAST of Lag0004664.1 vs. NCBI nr
Match: XP_023538510.1 (uncharacterized protein LOC111799267 [Cucurbita pepo subsp. pepo] >XP_023538511.1 uncharacterized protein LOC111799267 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 424.1 bits (1089), Expect = 7.9e-115
Identity = 196/218 (89.91%), Postives = 206/218 (94.50%), Query Frame = 0


Query: 1   MKDVFFRSLCFCLFFCFGFLFNWASSQIGAERAALPPRGWNSYDSFCWTVSEEEFLNNAE 60
           MKD  F SLCFCLFFCFG LFN  SSQ G ERAALPPRGWNSYDSFCWT+SE+EFL+NAE
Sbjct: 1   MKDFSFSSLCFCLFFCFGLLFNRVSSQTGPERAALPPRGWNSYDSFCWTISEKEFLDNAE 60

Query: 61  IVAKRLSSKGYEYVVVDYLWYRKKVPGAYTDSLGFDVIDEWGRMVPDPVRWPSSQGGKGF 120
           IVAKRL+SKGYEYVVVDYLWYRKKVPGAY DSLGFDVIDEWGRMVPDPVRWPSSQGGKGF
Sbjct: 61  IVAKRLNSKGYEYVVVDYLWYRKKVPGAYVDSLGFDVIDEWGRMVPDPVRWPSSQGGKGF 120

Query: 121 SEVAKKVHDMGLKFGIHIMRGISTQAVNANTPILDISKGGAYVESGRKWFASDIGIKSRA 180
           +EVAKKVHDMGLKFGIH+MRGISTQAVNANTPILD+SKGGAYVESGRKWFASDIGIKSR+
Sbjct: 121 TEVAKKVHDMGLKFGIHVMRGISTQAVNANTPILDVSKGGAYVESGRKWFASDIGIKSRS 180

Query: 181 CGWMRNGFMSVNVNSGAGKAFLRSLFQQYADWGVDFGK 219
           C WM NGFMSVNVNSGAGKAFLRSL+QQ+ADWGVDF K
Sbjct: 181 CAWMHNGFMSVNVNSGAGKAFLRSLYQQFADWGVDFVK 218

[Close]

BLAST of Lag0004664.1 vs. NCBI nr
Match: KAG7020103.1 (mel1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 422.5 bits (1085), Expect = 2.3e-114
Identity = 195/218 (89.45%), Postives = 206/218 (94.50%), Query Frame = 0


Query: 1   MKDVFFRSLCFCLFFCFGFLFNWASSQIGAERAALPPRGWNSYDSFCWTVSEEEFLNNAE 60
           MKD  F SLCFCLFFCFG LFN  SSQ G ERAALPPRGWNSYDSFCWT+SE+EFL+NAE
Sbjct: 1   MKDFSFSSLCFCLFFCFGLLFNRVSSQTGPERAALPPRGWNSYDSFCWTISEKEFLDNAE 60

Query: 61  IVAKRLSSKGYEYVVVDYLWYRKKVPGAYTDSLGFDVIDEWGRMVPDPVRWPSSQGGKGF 120
           IVAKRL+SKGYEYVVVDYLWYRKKVPGAY DSLGFDVIDEWGR+VPDPVRWPSSQGGKGF
Sbjct: 61  IVAKRLNSKGYEYVVVDYLWYRKKVPGAYVDSLGFDVIDEWGRIVPDPVRWPSSQGGKGF 120

Query: 121 SEVAKKVHDMGLKFGIHIMRGISTQAVNANTPILDISKGGAYVESGRKWFASDIGIKSRA 180
           +EVAKKVHDMGLKFGIH+MRGISTQAVNANTPILD+SKGGAYVESGRKWFASDIGIKSR+
Sbjct: 121 TEVAKKVHDMGLKFGIHVMRGISTQAVNANTPILDVSKGGAYVESGRKWFASDIGIKSRS 180

Query: 181 CGWMRNGFMSVNVNSGAGKAFLRSLFQQYADWGVDFGK 219
           C WM NGFMSVNVNSGAGKAFLRSL+QQ+ADWGVDF K
Sbjct: 181 CAWMHNGFMSVNVNSGAGKAFLRSLYQQFADWGVDFVK 218

[Close]

BLAST of Lag0004664.1 vs. NCBI nr
Match: XP_022951774.1 (uncharacterized protein LOC111454511 [Cucurbita moschata] >XP_022951775.1 uncharacterized protein LOC111454511 [Cucurbita moschata])

HSP 1 Score: 422.5 bits (1085), Expect = 2.3e-114
Identity = 195/218 (89.45%), Postives = 206/218 (94.50%), Query Frame = 0


Query: 1   MKDVFFRSLCFCLFFCFGFLFNWASSQIGAERAALPPRGWNSYDSFCWTVSEEEFLNNAE 60
           MKD  F SLCFCLFFCFG LFN  SSQ G ERAALPPRGWNSYDSFCWT+SE+EFL+NAE
Sbjct: 1   MKDFSFSSLCFCLFFCFGLLFNRVSSQTGPERAALPPRGWNSYDSFCWTISEKEFLDNAE 60

Query: 61  IVAKRLSSKGYEYVVVDYLWYRKKVPGAYTDSLGFDVIDEWGRMVPDPVRWPSSQGGKGF 120
           IVAKRL+SKGYEYVVVDYLWYRKKVPGAY DSLGFDVIDEWGR+VPDPVRWPSSQGGKGF
Sbjct: 61  IVAKRLNSKGYEYVVVDYLWYRKKVPGAYVDSLGFDVIDEWGRIVPDPVRWPSSQGGKGF 120

Query: 121 SEVAKKVHDMGLKFGIHIMRGISTQAVNANTPILDISKGGAYVESGRKWFASDIGIKSRA 180
           +EVAKKVHDMGLKFGIH+MRGISTQAVNANTPILD+SKGGAYVESGRKWFASDIGIKSR+
Sbjct: 121 TEVAKKVHDMGLKFGIHVMRGISTQAVNANTPILDVSKGGAYVESGRKWFASDIGIKSRS 180

Query: 181 CGWMRNGFMSVNVNSGAGKAFLRSLFQQYADWGVDFGK 219
           C WM NGFMSVNVNSGAGKAFLRSL+QQ+ADWGVDF K
Sbjct: 181 CAWMHNGFMSVNVNSGAGKAFLRSLYQQFADWGVDFVK 218

[Close]

BLAST of Lag0004664.1 vs. NCBI nr
Match: XP_023002641.1 (uncharacterized protein LOC111496438 [Cucurbita maxima] >XP_023002642.1 uncharacterized protein LOC111496438 [Cucurbita maxima])

HSP 1 Score: 420.2 bits (1079), Expect = 1.1e-113
Identity = 196/218 (89.91%), Postives = 206/218 (94.50%), Query Frame = 0


Query: 1   MKDVFFRSLCFCLFFCFGFLFNWASSQIGAERAALPPRGWNSYDSFCWTVSEEEFLNNAE 60
           MKD FF SLC CLFFCFG LFN  SSQ G ERAALPPRGWNSYDSFCWT+SE+EFL+NAE
Sbjct: 1   MKD-FFSSLCVCLFFCFGLLFNRVSSQTGPERAALPPRGWNSYDSFCWTISEKEFLDNAE 60

Query: 61  IVAKRLSSKGYEYVVVDYLWYRKKVPGAYTDSLGFDVIDEWGRMVPDPVRWPSSQGGKGF 120
           IVAKRL+SKGYEYVVVDYLWYRKKVPGAY DSLGFDVIDEWGRMVPDPVRWPSSQGGKGF
Sbjct: 61  IVAKRLNSKGYEYVVVDYLWYRKKVPGAYVDSLGFDVIDEWGRMVPDPVRWPSSQGGKGF 120

Query: 121 SEVAKKVHDMGLKFGIHIMRGISTQAVNANTPILDISKGGAYVESGRKWFASDIGIKSRA 180
           +EVAKKVHDMGLKFGIH+MRGISTQAVNANTPILD+SKGGAYVESGRKWFASDIGIKSR+
Sbjct: 121 TEVAKKVHDMGLKFGIHVMRGISTQAVNANTPILDVSKGGAYVESGRKWFASDIGIKSRS 180

Query: 181 CGWMRNGFMSVNVNSGAGKAFLRSLFQQYADWGVDFGK 219
           C WM NGFMSVNVNSGAGKAFLRSL+QQ+ADWGVDF K
Sbjct: 181 CAWMHNGFMSVNVNSGAGKAFLRSLYQQFADWGVDFVK 217

[Close]

BLAST of Lag0004664.1 vs. NCBI nr
Match: XP_038886461.1 (alpha-galactosidase mel1 [Benincasa hispida])

HSP 1 Score: 416.8 bits (1070), Expect = 1.3e-112
Identity = 194/218 (88.99%), Postives = 200/218 (91.74%), Query Frame = 0


Query: 1   MKDVFFRSLCFCLFFCFGFLFNWASSQIGAERAALPPRGWNSYDSFCWTVSEEEFLNNAE 60
           M++VFF SLCF LFFCFGF FNW SSQ G ERAALPPRGWNSYDSF W +SEEEFL NAE
Sbjct: 2   MENVFFSSLCFSLFFCFGFFFNWVSSQTGPERAALPPRGWNSYDSFSWIISEEEFLQNAE 61

Query: 61  IVAKRLSSKGYEYVVVDYLWYRKKVPGAYTDSLGFDVIDEWGRMVPDPVRWPSSQGGKGF 120
           IVA RL   GYEYVV DYLWYRKKVPGAYTDSLGFDVIDEWGRMVPDPVRWPSSQGGKGF
Sbjct: 62  IVANRLKPMGYEYVVADYLWYRKKVPGAYTDSLGFDVIDEWGRMVPDPVRWPSSQGGKGF 121

Query: 121 SEVAKKVHDMGLKFGIHIMRGISTQAVNANTPILDISKGGAYVESGRKWFASDIGIKSRA 180
           S+VAKKVHDMGLKFGIH+MRGISTQAVNANTPILDISKG AYVESGRKWFASDIGIKSRA
Sbjct: 122 SKVAKKVHDMGLKFGIHVMRGISTQAVNANTPILDISKGDAYVESGRKWFASDIGIKSRA 181

Query: 181 CGWMRNGFMSVNVNSGAGKAFLRSLFQQYADWGVDFGK 219
           CGWM NGFMSVNV SGAGKAFLRSL+QQYADWGVDF K
Sbjct: 182 CGWMHNGFMSVNVKSGAGKAFLRSLYQQYADWGVDFVK 219

[Close]

BLAST of Lag0004664.1 vs. ExPASy Swiss-Prot
Match: Q9URZ0 (Alpha-galactosidase mel1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mel1 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 8.5e-11
Identity = 58/208 (27.88%), Postives = 84/208 (40.38%), Query Frame = 0


Query: 10  CFCLFFCFGFLFNWASSQIGAERAALPPRGWNSYDSFCWTVSEEEFLNNAEIVAKR-LSS 69
           CF L F F F  +   S  G      P  GWNS++ +   + E   LNNA+ + +  L  
Sbjct: 9   CFFLVFLFLFFSDVHGSYNGL--GLKPQMGWNSWNKYACDIDESIILNNAKAIKEEGLLD 68

Query: 70  KGYEYVVVDYLWYRKKVPGAYTDSLGFDVIDEWGRMVPDPVRWPSSQGGKGFSEVAKKVH 129
            GYEY+V+D  W  K    A T           GR+  +P ++P+     G   +AKK+H
Sbjct: 69  LGYEYIVMDDCW-SKHERNATT-----------GRLEANPDKFPN-----GIGSMAKKLH 128

Query: 130 DMGLKFGIHIMRGISTQAVNANTPILDISKGGAYVESGRKWFASDIGIKSRACGWMRNGF 189
           DMG KFG++   G  T A    +   +      + + G  +   D        G     +
Sbjct: 129 DMGFKFGMYSSAGKYTCAGFPGSLNHEQIDADTFADWGVDYLKYDNCFNEGKSGVPLISY 188

Query: 190 MSVNVNSGAGKAFLRSLFQQYADWGVDF 217
                 S A     R +F     WG DF
Sbjct: 189 ERYKRMSDALNKTGRPIFYSLCQWGEDF 197

[Close]

BLAST of Lag0004664.1 vs. ExPASy Swiss-Prot
Match: A7XZT2 (Probable alpha-galactosidase B OS=Talaromyces emersonii OX=68825 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.1e-10
Identity = 42/113 (37.17%), Postives = 60/113 (53.10%), Query Frame = 0


Query: 35  LPPRGWNSYDSFCWTVSEEEFLNNA-EIVAKRLSSKGYEYVVVDYLWYRKKVPGAYTDSL 94
           LP  GWNS+++F   + EE+ L  A +IV   L   GYEYV +D  W  K    A T   
Sbjct: 34  LPALGWNSWNAFGCDIDEEKILTAANQIVNLGLKDLGYEYVNIDDCWSVKSGRNATT--- 93

Query: 95  GFDVIDEWGRMVPDPVRWPSSQGGKGFSEVAKKVHDMGLKFGIHIMRGISTQA 147
                   GR++PD  ++P      G S +A+K+H++GLK GI+   G +T A
Sbjct: 94  --------GRIMPDLTKFPD-----GISGLAEKIHNLGLKIGIYSSAGWTTCA 130

[Close]

BLAST of Lag0004664.1 vs. ExPASy Swiss-Prot
Match: B0Y224 (Probable alpha-galactosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=aglB PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.4e-10
Identity = 39/122 (31.97%), Postives = 61/122 (50.00%), Query Frame = 0


Query: 26  SQIGAERAALPPRGWNSYDSFCWTVSEEEFLNNA-EIVAKRLSSKGYEYVVVDYLWYRKK 85
           S+    +  LP  GWN++++F   +   + +  A E+V   L   GYEY+ +D  W  K 
Sbjct: 2   SRSKTRQGKLPALGWNTWNAFGCDIDATKIMTAANEVVNLGLKDLGYEYINIDDCWSVKS 61

Query: 86  VPGAYTDSLGFDVIDEWGRMVPDPVRWPSSQGGKGFSEVAKKVHDMGLKFGIHIMRGIST 145
              A T            R++PDP ++P      G S VA ++HD+GLK GI+   G++T
Sbjct: 62  GRDASTQ-----------RIIPDPDKFPD-----GISGVADQIHDLGLKIGIYSSAGLTT 107

Query: 146 QA 147
            A
Sbjct: 122 CA 107

[Close]

BLAST of Lag0004664.1 vs. ExPASy Swiss-Prot
Match: A1D0A3 (Probable alpha-galactosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=aglB PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.4e-10
Identity = 40/113 (35.40%), Postives = 58/113 (51.33%), Query Frame = 0


Query: 35  LPPRGWNSYDSFCWTVSEEEFLNNA-EIVAKRLSSKGYEYVVVDYLWYRKKVPGAYTDSL 94
           LP  GWNS+++F   +   + +  A E+V   L   GYEY+ +D  W  K    A T   
Sbjct: 32  LPALGWNSWNAFGCDIDAAKIMTAANEVVNLGLKDLGYEYINIDDCWSVKSGRDASTQ-- 91

Query: 95  GFDVIDEWGRMVPDPVRWPSSQGGKGFSEVAKKVHDMGLKFGIHIMRGISTQA 147
                    RMVPDP ++P      G S +A ++HD+GLK GI+   G++T A
Sbjct: 92  ---------RMVPDPEKFPD-----GISGLADQIHDLGLKVGIYSSAGLTTCA 128

[Close]

BLAST of Lag0004664.1 vs. ExPASy Swiss-Prot
Match: A1C5D3 (Probable alpha-galactosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) OX=344612 GN=aglB PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.2e-10
Identity = 40/113 (35.40%), Postives = 60/113 (53.10%), Query Frame = 0


Query: 35  LPPRGWNSYDSFCWTVSEEEFLNNA-EIVAKRLSSKGYEYVVVDYLWYRKKVPGAYTDSL 94
           LP  GWNS+++F   + + + +  A EIV   L   GYEY+ +D  W  K          
Sbjct: 33  LPALGWNSWNAFGCDIDDAKIMTAAKEIVNLGLKDLGYEYINIDDCWSVKS--------- 92

Query: 95  GFDVIDEWGRMVPDPVRWPSSQGGKGFSEVAKKVHDMGLKFGIHIMRGISTQA 147
           G D   +  R+VPDP ++P      G + VA ++HD+GLK GI+   G++T A
Sbjct: 93  GRDKTTK--RIVPDPAKFPD-----GIAGVADRIHDLGLKVGIYSSAGLTTCA 129

[Close]

BLAST of Lag0004664.1 vs. ExPASy TrEMBL
Match: A0A6J1GJU2 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111454511 PE=3 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 1.1e-114
Identity = 195/218 (89.45%), Postives = 206/218 (94.50%), Query Frame = 0


Query: 1   MKDVFFRSLCFCLFFCFGFLFNWASSQIGAERAALPPRGWNSYDSFCWTVSEEEFLNNAE 60
           MKD  F SLCFCLFFCFG LFN  SSQ G ERAALPPRGWNSYDSFCWT+SE+EFL+NAE
Sbjct: 1   MKDFSFSSLCFCLFFCFGLLFNRVSSQTGPERAALPPRGWNSYDSFCWTISEKEFLDNAE 60

Query: 61  IVAKRLSSKGYEYVVVDYLWYRKKVPGAYTDSLGFDVIDEWGRMVPDPVRWPSSQGGKGF 120
           IVAKRL+SKGYEYVVVDYLWYRKKVPGAY DSLGFDVIDEWGR+VPDPVRWPSSQGGKGF
Sbjct: 61  IVAKRLNSKGYEYVVVDYLWYRKKVPGAYVDSLGFDVIDEWGRIVPDPVRWPSSQGGKGF 120

Query: 121 SEVAKKVHDMGLKFGIHIMRGISTQAVNANTPILDISKGGAYVESGRKWFASDIGIKSRA 180
           +EVAKKVHDMGLKFGIH+MRGISTQAVNANTPILD+SKGGAYVESGRKWFASDIGIKSR+
Sbjct: 121 TEVAKKVHDMGLKFGIHVMRGISTQAVNANTPILDVSKGGAYVESGRKWFASDIGIKSRS 180

Query: 181 CGWMRNGFMSVNVNSGAGKAFLRSLFQQYADWGVDFGK 219
           C WM NGFMSVNVNSGAGKAFLRSL+QQ+ADWGVDF K
Sbjct: 181 CAWMHNGFMSVNVNSGAGKAFLRSLYQQFADWGVDFVK 218

[Close]

BLAST of Lag0004664.1 vs. ExPASy TrEMBL
Match: A0A6J1KK35 (Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111496438 PE=3 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 5.5e-114
Identity = 196/218 (89.91%), Postives = 206/218 (94.50%), Query Frame = 0


Query: 1   MKDVFFRSLCFCLFFCFGFLFNWASSQIGAERAALPPRGWNSYDSFCWTVSEEEFLNNAE 60
           MKD FF SLC CLFFCFG LFN  SSQ G ERAALPPRGWNSYDSFCWT+SE+EFL+NAE
Sbjct: 1   MKD-FFSSLCVCLFFCFGLLFNRVSSQTGPERAALPPRGWNSYDSFCWTISEKEFLDNAE 60

Query: 61  IVAKRLSSKGYEYVVVDYLWYRKKVPGAYTDSLGFDVIDEWGRMVPDPVRWPSSQGGKGF 120
           IVAKRL+SKGYEYVVVDYLWYRKKVPGAY DSLGFDVIDEWGRMVPDPVRWPSSQGGKGF
Sbjct: 61  IVAKRLNSKGYEYVVVDYLWYRKKVPGAYVDSLGFDVIDEWGRMVPDPVRWPSSQGGKGF 120

Query: 121 SEVAKKVHDMGLKFGIHIMRGISTQAVNANTPILDISKGGAYVESGRKWFASDIGIKSRA 180
           +EVAKKVHDMGLKFGIH+MRGISTQAVNANTPILD+SKGGAYVESGRKWFASDIGIKSR+
Sbjct: 121 TEVAKKVHDMGLKFGIHVMRGISTQAVNANTPILDVSKGGAYVESGRKWFASDIGIKSRS 180

Query: 181 CGWMRNGFMSVNVNSGAGKAFLRSLFQQYADWGVDFGK 219
           C WM NGFMSVNVNSGAGKAFLRSL+QQ+ADWGVDF K
Sbjct: 181 CAWMHNGFMSVNVNSGAGKAFLRSLYQQFADWGVDFVK 217

[Close]

BLAST of Lag0004664.1 vs. ExPASy TrEMBL
Match: A0A6J1BQ77 (Alpha-galactosidase OS=Momordica charantia OX=3673 GN=LOC111004803 PE=3 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 6.1e-113
Identity = 195/218 (89.45%), Postives = 203/218 (93.12%), Query Frame = 0


Query: 1   MKDVFFRSLCFCLFFCFGFLFNWASSQIGAERAALPPRGWNSYDSFCWTVSEEEFLNNAE 60
           MK VFF SL F LFFCFGFL +W SS+ G ERAA PPRGWNSYDSFCWT+SEEEFL NAE
Sbjct: 1   MKGVFFSSLYFYLFFCFGFLCSWVSSKNGPERAAFPPRGWNSYDSFCWTISEEEFLQNAE 60

Query: 61  IVAKRLSSKGYEYVVVDYLWYRKKVPGAYTDSLGFDVIDEWGRMVPDPVRWPSSQGGKGF 120
           IVAKRL SKGYEYVVVDYLWYRKKVPGAYTDSLGFDVIDEWGRMVPDP RWPSS+GGKG 
Sbjct: 61  IVAKRLKSKGYEYVVVDYLWYRKKVPGAYTDSLGFDVIDEWGRMVPDPGRWPSSRGGKGL 120

Query: 121 SEVAKKVHDMGLKFGIHIMRGISTQAVNANTPILDISKGGAYVESGRKWFASDIGIKSRA 180
           SEVAKKVHDMGLKFGIH+MRGISTQAVNANTPILD+SKGGAYVESGRKWFASDIGIKSRA
Sbjct: 121 SEVAKKVHDMGLKFGIHVMRGISTQAVNANTPILDVSKGGAYVESGRKWFASDIGIKSRA 180

Query: 181 CGWMRNGFMSVNVNSGAGKAFLRSLFQQYADWGVDFGK 219
           CGWMRNGFMSVNVNSGAGKAFLRSL+ QYA+WGVDF K
Sbjct: 181 CGWMRNGFMSVNVNSGAGKAFLRSLYLQYAEWGVDFVK 218

[Close]

BLAST of Lag0004664.1 vs. ExPASy TrEMBL
Match: A0A1S3B9Q1 (Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103487727 PE=3 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.7e-108
Identity = 185/212 (87.26%), Postives = 194/212 (91.51%), Query Frame = 0


Query: 7   RSLCFCLFFCFGFLFNWASSQIGAERAALPPRGWNSYDSFCWTVSEEEFLNNAEIVAKRL 66
           +++CF LFF FG LFNW SSQ G ERAALPPRGWNSYDSF W +SEEEFLNN EIVA +L
Sbjct: 2   KNVCFSLFFSFGLLFNWVSSQTGPERAALPPRGWNSYDSFSWIISEEEFLNNVEIVANKL 61

Query: 67  SSKGYEYVVVDYLWYRKKVPGAYTDSLGFDVIDEWGRMVPDPVRWPSSQGGKGFSEVAKK 126
            SKGYEYV+VDYLWYRKKVPGAYTDSLGFDVIDEWGRM PDPVRWPSSQGGKGFSEVAKK
Sbjct: 62  KSKGYEYVIVDYLWYRKKVPGAYTDSLGFDVIDEWGRMAPDPVRWPSSQGGKGFSEVAKK 121

Query: 127 VHDMGLKFGIHIMRGISTQAVNANTPILDISKGGAYVESGRKWFASDIGIKSRACGWMRN 186
           VH MGLKFGIH+MRGISTQAVNANTPILDISKG AYVESG+KW ASDIGIKSRACGWM N
Sbjct: 122 VHAMGLKFGIHVMRGISTQAVNANTPILDISKGDAYVESGKKWLASDIGIKSRACGWMHN 181

Query: 187 GFMSVNVNSGAGKAFLRSLFQQYADWGVDFGK 219
           GFMSVNV SGAGKAFLRSL+QQYADWGVDF K
Sbjct: 182 GFMSVNVKSGAGKAFLRSLYQQYADWGVDFVK 213

[Close]

BLAST of Lag0004664.1 vs. ExPASy TrEMBL
Match: A0A5A7UYS7 (Alpha-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001900 PE=3 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.7e-108
Identity = 185/212 (87.26%), Postives = 194/212 (91.51%), Query Frame = 0


Query: 7   RSLCFCLFFCFGFLFNWASSQIGAERAALPPRGWNSYDSFCWTVSEEEFLNNAEIVAKRL 66
           +++CF LFF FG LFNW SSQ G ERAALPPRGWNSYDSF W +SEEEFLNN EIVA +L
Sbjct: 2   KNVCFSLFFSFGLLFNWVSSQTGPERAALPPRGWNSYDSFSWIISEEEFLNNVEIVANKL 61

Query: 67  SSKGYEYVVVDYLWYRKKVPGAYTDSLGFDVIDEWGRMVPDPVRWPSSQGGKGFSEVAKK 126
            SKGYEYV+VDYLWYRKKVPGAYTDSLGFDVIDEWGRM PDPVRWPSSQGGKGFSEVAKK
Sbjct: 62  KSKGYEYVIVDYLWYRKKVPGAYTDSLGFDVIDEWGRMAPDPVRWPSSQGGKGFSEVAKK 121

Query: 127 VHDMGLKFGIHIMRGISTQAVNANTPILDISKGGAYVESGRKWFASDIGIKSRACGWMRN 186
           VH MGLKFGIH+MRGISTQAVNANTPILDISKG AYVESG+KW ASDIGIKSRACGWM N
Sbjct: 122 VHAMGLKFGIHVMRGISTQAVNANTPILDISKGDAYVESGKKWLASDIGIKSRACGWMHN 181

Query: 187 GFMSVNVNSGAGKAFLRSLFQQYADWGVDFGK 219
           GFMSVNV SGAGKAFLRSL+QQYADWGVDF K
Sbjct: 182 GFMSVNVKSGAGKAFLRSLYQQYADWGVDFVK 213

[Close]

BLAST of Lag0004664.1 vs. TAIR 10
Match: AT3G26380.1 (Melibiase family protein )

HSP 1 Score: 312.8 bits (800), Expect = 2.4e-85
Identity = 146/212 (68.87%), Postives = 168/212 (79.25%), Query Frame = 0


Query: 7   RSLCFCLFFCFGFLFNWASSQIGAERAALPPRGWNSYDSFCWTVSEEEFLNNAEIVAKRL 66
           RS  F + F          ++   + A+ PPRGWNSYDSFCWT+SE EFL +AEIV+KRL
Sbjct: 13  RSTVFFIIFNLSIFSLSIEARSRQQHASFPPRGWNSYDSFCWTISEAEFLQSAEIVSKRL 72

Query: 67  SSKGYEYVVVDYLWYRKKVPGAYTDSLGFDVIDEWGRMVPDPVRWPSSQGGKGFSEVAKK 126
              GY+YVVVDYLWYRKKV GAY DSLGFDVIDEWGR+ PDP RWPSS+GGKGF+EVA+K
Sbjct: 73  LPHGYQYVVVDYLWYRKKVEGAYVDSLGFDVIDEWGRLHPDPGRWPSSRGGKGFTEVAEK 132

Query: 127 VHDMGLKFGIHIMRGISTQAVNANTPILDISKGGAYVESGRKWFASDIGIKSRACGWMRN 186
           VH MGLKFGIH+M GISTQA NAN+ ++D  KGGAY ESGR+W A DIGIK RAC WM +
Sbjct: 133 VHRMGLKFGIHVMGGISTQAYNANSLVMDSVKGGAYEESGRQWRAKDIGIKERACVWMSH 192

Query: 187 GFMSVNVNSGAGKAFLRSLFQQYADWGVDFGK 219
           GFMSVN   GAGKAFLRSL++QYA+WGVDF K
Sbjct: 193 GFMSVNTKLGAGKAFLRSLYRQYAEWGVDFIK 224

The following BLAST results are available for this feature:

BLAST of Lag0004664.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023538510.1	7.9e-115	89.91	uncharacterized protein LOC111799267 [Cucurbita pepo subsp. pepo] >XP_023538511....	[more]
KAG7020103.1	2.3e-114	89.45	mel1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022951774.1	2.3e-114	89.45	uncharacterized protein LOC111454511 [Cucurbita moschata] >XP_022951775.1 unchar...	[more]
XP_023002641.1	1.1e-113	89.91	uncharacterized protein LOC111496438 [Cucurbita maxima] >XP_023002642.1 uncharac...	[more]
XP_038886461.1	1.3e-112	88.99	alpha-galactosidase mel1 [Benincasa hispida]	[more]

BLAST of Lag0004664.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9URZ0	8.5e-11	27.88	Alpha-galactosidase mel1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ...	[more]
A7XZT2	1.1e-10	37.17	Probable alpha-galactosidase B OS=Talaromyces emersonii OX=68825 PE=3 SV=1	[more]
B0Y224	1.4e-10	31.97	Probable alpha-galactosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.8...	[more]
A1D0A3	1.4e-10	35.40	Probable alpha-galactosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3...	[more]
A1C5D3	3.2e-10	35.40	Probable alpha-galactosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 5...	[more]

BLAST of Lag0004664.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GJU2	1.1e-114	89.45	Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111454511 PE=3 SV=1	[more]
A0A6J1KK35	5.5e-114	89.91	Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111496438 PE=3 SV=1	[more]
A0A6J1BQ77	6.1e-113	89.45	Alpha-galactosidase OS=Momordica charantia OX=3673 GN=LOC111004803 PE=3 SV=1	[more]
A0A1S3B9Q1	2.7e-108	87.26	Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103487727 PE=3 SV=1	[more]
A0A5A7UYS7	2.7e-108	87.26	Alpha-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G...	[more]

BLAST of Lag0004664.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26380.1	2.4e-85	68.87	Melibiase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002241	Glycoside hydrolase, family 27	PFAM	PF16499	Melibiase_2	coord: 50..144 e-value: 3.5E-5 score: 23.1
IPR002241	Glycoside hydrolase, family 27	PANTHER	PTHR11452	ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE	coord: 7..218
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 26..231 e-value: 2.2E-60 score: 206.6
None	No IPR available	PANTHER	PTHR11452:SF42	ALPHA-GALACTOSIDASE	coord: 7..218
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 32..218

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lag0004664	Lag0004664	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lag0004664.1.exon1	Lag0004664.1.exon1	exon
Lag0004664.1.exon2	Lag0004664.1.exon2	exon
Lag0004664.1.exon3	Lag0004664.1.exon3	exon
Lag0004664.1.exon4	Lag0004664.1.exon4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Lag0004664.1	cds.Lag0004664.1	CDS
cds.Lag0004664.1	cds.Lag0004664.1_2	CDS
cds.Lag0004664.1	cds.Lag0004664.1_3	CDS
cds.Lag0004664.1	cds.Lag0004664.1_4	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lag0004664.1	Lag0004664.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0052692	raffinose alpha-galactosidase activity